##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename 000000000-AHV5L l01n01 kokuya7.34100000033d3e.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2802850 Sequences flagged as poor quality 0 Sequence length 76 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 33.6434893055283 34.0 34.0 34.0 34.0 34.0 2 33.83263999143729 34.0 34.0 34.0 34.0 34.0 3 33.8261776406158 34.0 34.0 34.0 34.0 34.0 4 33.873490197477565 34.0 34.0 34.0 34.0 34.0 5 33.906366733860175 34.0 34.0 34.0 34.0 34.0 6 37.726630750842894 38.0 38.0 38.0 38.0 38.0 7 37.73711793353194 38.0 38.0 38.0 38.0 38.0 8 37.763897818292094 38.0 38.0 38.0 38.0 38.0 9 37.73547068162763 38.0 38.0 38.0 38.0 38.0 10-11 37.547208198797655 38.0 38.0 38.0 37.0 38.0 12-13 37.50930410118272 38.0 38.0 38.0 37.0 38.0 14-15 37.51085948231265 38.0 38.0 38.0 37.0 38.0 16-17 37.72223897104733 38.0 38.0 38.0 37.5 38.0 18-19 37.687636512835155 38.0 38.0 38.0 38.0 38.0 20-21 37.6124505414132 38.0 38.0 38.0 37.0 38.0 22-23 37.559905274987955 38.0 38.0 38.0 37.0 38.0 24-25 37.68806161585529 38.0 38.0 38.0 37.5 38.0 26-27 37.26745509035446 38.0 38.0 38.0 37.0 38.0 28-29 37.56126103787217 38.0 38.0 38.0 37.5 38.0 30-31 37.489982339404534 38.0 38.0 38.0 37.0 38.0 32-33 37.37990420464884 38.0 38.0 38.0 37.0 38.0 34-35 37.49811548959095 38.0 38.0 38.0 37.0 38.0 36-37 37.28619601477068 38.0 38.0 38.0 37.0 38.0 38-39 37.631169167097774 38.0 38.0 38.0 37.5 38.0 40-41 37.25276914569099 38.0 38.0 38.0 37.0 38.0 42-43 37.176728686872295 38.0 38.0 38.0 37.0 38.0 44-45 37.3430411902171 38.0 38.0 38.0 37.0 38.0 46-47 37.330743172128365 38.0 38.0 38.0 37.0 38.0 48-49 37.32292416647341 38.0 38.0 38.0 37.0 38.0 50-51 37.15333749576324 38.0 38.0 38.0 37.0 38.0 52-53 37.21449221328291 38.0 38.0 38.0 37.0 38.0 54-55 37.1764912499777 38.0 38.0 38.0 37.0 38.0 56-57 37.34770840394599 38.0 38.0 38.0 37.0 38.0 58-59 37.38766701750004 38.0 38.0 38.0 37.0 38.0 60-61 37.32852025616783 38.0 38.0 38.0 37.0 38.0 62-63 37.16049521023244 38.0 38.0 38.0 37.0 38.0 64-65 37.292080025688136 38.0 38.0 38.0 37.0 38.0 66-67 37.40743689458944 38.0 38.0 38.0 37.0 38.0 68-69 37.509014752840855 38.0 38.0 38.0 37.0 38.0 70-71 37.43968603385839 38.0 38.0 38.0 37.0 38.0 72-73 37.125729703694454 38.0 38.0 38.0 37.0 38.0 74-75 37.21533225110156 38.0 38.0 38.0 37.0 38.0 76 36.35286012451612 38.0 37.0 38.0 34.0 38.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.029174404071454774 1101 2 0.008749087878349826 1101 3 0.0330444378466197 1101 4 -6.0391483707178395E-5 1101 5 0.01797037718966976 1101 6 -0.02395115130617853 1101 7 -0.03385627861924689 1101 8 -0.08466728283038805 1101 9 -0.41977909982957584 1101 10-11 -0.49494786126834356 1101 12-13 -0.7951469191364424 1101 14-15 -0.37661491884457376 1101 16-17 -0.06995610830465182 1101 18-19 0.04085831786638039 1101 20-21 0.14142369214161477 1101 22-23 0.11203101517956071 1101 24-25 0.09147057023204752 1101 26-27 0.1297458609354507 1101 28-29 0.10102542692912664 1101 30-31 0.15129211330981462 1101 32-33 0.13700414146883588 1101 34-35 0.13379262737494457 1101 36-37 0.12209314173612995 1101 38-39 0.05998247177292626 1101 40-41 0.17810507421620514 1101 42-43 0.15104261471072533 1101 44-45 0.17717216106797906 1101 46-47 0.08987628738551479 1101 48-49 0.11389325731458655 1101 50-51 0.2117718099194903 1101 52-53 0.22813320863151176 1101 54-55 0.22439665377580553 1101 56-57 0.13214694441807495 1101 58-59 0.1720905016801666 1101 60-61 0.1455100850244193 1101 62-63 0.10886285658138206 1101 64-65 0.11831343462856836 1101 66-67 0.20499699895056978 1101 68-69 0.0913054394604913 1101 70-71 0.09557964626669246 1101 72-73 0.12022663904667752 1101 74-75 0.16959055526906752 1101 76 0.1372708216501266 1102 1 0.07492849448219374 1102 2 0.023845050470526985 1102 3 0.04414498467746597 1102 4 0.018674359368951343 1102 5 0.039638552804369453 1102 6 -0.031184921931462384 1102 7 0.014572085223100828 1102 8 -0.03459017861590041 1102 9 -0.2511165975011309 1102 10-11 -0.18088326379812258 1102 12-13 0.027913186800148537 1102 14-15 0.14449141998731108 1102 16-17 0.03916520320521499 1102 18-19 0.06345061468417867 1102 20-21 0.13684108411695206 1102 22-23 0.10079443050862835 1102 24-25 0.07073185063771348 1102 26-27 0.0751667748967435 1102 28-29 0.02812897406120385 1102 30-31 0.07517625927773963 1102 32-33 0.1407634060081122 1102 34-35 0.0767242250123985 1102 36-37 0.07214650903353004 1102 38-39 0.04039904208575251 1102 40-41 0.11773766112404616 1102 42-43 0.13231302755854557 1102 44-45 0.12218527354551156 1102 46-47 0.06864502323855959 1102 48-49 0.06720024913212086 1102 50-51 0.13641716385028957 1102 52-53 0.21071355062711206 1102 54-55 0.16563117558362705 1102 56-57 0.1325043872400542 1102 58-59 0.1450265912337514 1102 60-61 0.1325138662422347 1102 62-63 0.1629388693526579 1102 64-65 0.13197130160475723 1102 66-67 0.1703178985763074 1102 68-69 0.09157632738888566 1102 70-71 0.07094812058485189 1102 72-73 0.15423176075307765 1102 74-75 0.2279971009167241 1102 76 0.45770003729167996 1103 1 0.020096923718796234 1103 2 0.031215919277855164 1103 3 0.03656479508411081 1103 4 0.011832608684684942 1103 5 0.017184406967764687 1103 6 0.07164804032490224 1103 7 0.02679850198133238 1103 8 0.050456180731067946 1103 9 0.1005102884718383 1103 10-11 0.19562204011166529 1103 12-13 0.2203655311503212 1103 14-15 0.17562933333496034 1103 16-17 0.07093120071528602 1103 18-19 0.0665463028465112 1103 20-21 0.1514925951547923 1103 22-23 0.11162195468769909 1103 24-25 0.06400340845574704 1103 26-27 0.15786611655637728 1103 28-29 0.06138996778742012 1103 30-31 0.09785431197200722 1103 32-33 0.11184924433938193 1103 34-35 0.11075529632920933 1103 36-37 -0.021282212945223478 1103 38-39 0.05950761812340488 1103 40-41 0.10994379623893735 1103 42-43 0.1176572838193195 1103 44-45 0.060188409276854316 1103 46-47 0.09004075012250468 1103 48-49 -0.01214630582045828 1103 50-51 0.13914214045471596 1103 52-53 0.05635545775690076 1103 54-55 0.16264191397747396 1103 56-57 0.13091115581068635 1103 58-59 0.14528091872936244 1103 60-61 0.1542780513034927 1103 62-63 0.2049086918362022 1103 64-65 0.21107001514368307 1103 66-67 0.16914291285481653 1103 68-69 0.08528674630208144 1103 70-71 0.14894222086905984 1103 72-73 0.22245578137601996 1103 74-75 0.2060381202389081 1103 76 0.40227820261843306 2116 1 0.022648495281302417 2116 2 0.0416855460620269 2116 3 0.022067049194134825 2116 4 0.02900977371906066 2116 5 0.024661296004275357 2116 6 0.07108284924302666 2116 7 0.05861839178039929 2116 8 0.052255957542882925 2116 9 0.09723722047816352 2116 10-11 0.20724880785579103 2116 12-13 0.18949041091271113 2116 14-15 0.155691835653343 2116 16-17 0.06290419896619426 2116 18-19 0.06963680222794721 2116 20-21 0.1398847284651339 2116 22-23 0.14027913882984677 2116 24-25 0.07177259132706126 2116 26-27 0.1273273218235289 2116 28-29 0.05532577329373112 2116 30-31 0.14502603463535024 2116 32-33 0.0669137217117779 2116 34-35 0.16560141342966972 2116 36-37 0.06814334790639975 2116 38-39 0.10346998421261588 2116 40-41 0.11353407631150958 2116 42-43 0.14390143334124872 2116 44-45 0.1449544000644707 2116 46-47 0.12179976012065907 2116 48-49 0.12429984004923966 2116 50-51 0.1874813394860766 2116 52-53 0.1749536989252931 2116 54-55 0.18701535464047225 2116 56-57 0.12037576468345179 2116 58-59 0.1425695022942577 2116 60-61 0.16244712951817775 2116 62-63 0.15956116869550385 2116 64-65 0.1895010095883336 2116 66-67 0.20667884963497585 2116 68-69 0.021984000113370428 2116 70-71 0.16157853145119105 2116 72-73 0.24484185049186635 2116 74-75 0.16919486817019447 2116 76 0.2817877322894091 1117 1 -0.020169854121995456 1117 2 -0.06014105377443002 1117 3 -0.02950915653111963 1117 4 0.0059845936393330135 1117 5 0.03575056518103992 1117 6 0.06887393364259964 1117 7 0.09409236529803877 1117 8 0.04094086144988296 1117 9 0.11188020513930752 1117 10-11 0.17246574435646522 1117 12-13 0.21346262348251344 1117 14-15 0.17656962770608686 1117 16-17 0.0847605444355537 1117 18-19 0.027797115827290497 1117 20-21 0.15265299506124563 1117 22-23 0.07255484412551994 1117 24-25 -0.04504921262632422 1117 26-27 0.11780630840382855 1117 28-29 0.10431681473558285 1117 30-31 -0.015494692266706522 1117 32-33 0.13498908023741052 1117 34-35 0.15320188389245004 1117 36-37 0.1200550363122801 1117 38-39 0.043704713499266745 1117 40-41 0.1442220294995451 1117 42-43 -0.1729748547926988 1117 44-45 0.010560157754220256 1117 46-47 -0.08303653030861113 1117 48-49 -0.004825914500372619 1117 50-51 -0.14745392904352173 1117 52-53 0.01060061911956467 1117 54-55 -0.028496975979372507 1117 56-57 0.03922985148515323 1117 58-59 0.09010253152303704 1117 60-61 0.14806571674308344 1117 62-63 0.10317756340286621 1117 64-65 0.16035078746741505 1117 66-67 0.14501220156471817 1117 68-69 0.16938011654394103 1117 70-71 0.1353377884818272 1117 72-73 0.09008915427708786 1117 74-75 0.2620009738254794 1117 76 0.46874386679569824 2117 1 -0.005673642126538425 2117 2 0.011904725588529175 2117 3 0.00782814187542158 2117 4 0.009137522465636039 2117 5 3.2588497177954423E-4 2117 6 -0.02520823807289929 2117 7 0.020755170445049487 2117 8 -0.04483475461466924 2117 9 -0.20626445126053738 2117 10-11 -0.289139426522695 2117 12-13 -0.8033611817388078 2117 14-15 -1.2181199527518558 2117 16-17 -0.11357565243002199 2117 18-19 0.05266461076423212 2117 20-21 0.14695132492434482 2117 22-23 0.14511978445180063 2117 24-25 0.07494892380507423 2117 26-27 0.14833458563643376 2117 28-29 0.14183409671584002 2117 30-31 0.13458772568490218 2117 32-33 0.17715991829389566 2117 34-35 0.18478997436504585 2117 36-37 0.16884242230456437 2117 38-39 0.0931980916056716 2117 40-41 0.24696015127535986 2117 42-43 0.24688414638196576 2117 44-45 0.1993949807567006 2117 46-47 0.05312278721817876 2117 48-49 0.18956921026188667 2117 50-51 0.2334499669412864 2117 52-53 0.24669659902859564 2117 54-55 0.20196000937925618 2117 56-57 0.1078290862165332 2117 58-59 0.1793379328444118 2117 60-61 0.1969896491534442 2117 62-63 0.18047850740015292 2117 64-65 0.15357654763739959 2117 66-67 0.20259864506163439 2117 68-69 0.2112106507695799 2117 70-71 0.18246177646329187 2117 72-73 0.1010376395495598 2117 74-75 0.24253995224997738 2117 76 0.3555662234530885 1116 1 0.08384318253811074 1116 2 0.009166904881567461 1116 3 0.01720108471098314 1116 4 0.036918150876992684 1116 5 -0.014313995134251911 1116 6 -0.05209406237664638 1116 7 0.039146477714730565 1116 8 -0.10261374141886392 1116 9 -0.19416277131740145 1116 10-11 -0.2618388176412765 1116 12-13 -0.022905378647543273 1116 14-15 0.1333006655439064 1116 16-17 0.029933272047365733 1116 18-19 0.05020428706172453 1116 20-21 0.1520836778371688 1116 22-23 0.11584083917973231 1116 24-25 0.04023985449894241 1116 26-27 0.09918180818081623 1116 28-29 0.11254318573954691 1116 30-31 0.10702984422435691 1116 32-33 0.10223819178906268 1116 34-35 0.09313645655633707 1116 36-37 0.05337906124120906 1116 38-39 -4.2660702305852283E-4 1116 40-41 0.1622010257711537 1116 42-43 0.17598745817284822 1116 44-45 0.20929544816056733 1116 46-47 0.08125824248828195 1116 48-49 0.10872458936372453 1116 50-51 0.21089681084070833 1116 52-53 0.1831890909566667 1116 54-55 0.1164188595112634 1116 56-57 0.1516579998619747 1116 58-59 0.12867312368437211 1116 60-61 0.13733840729322822 1116 62-63 0.020348504197841066 1116 64-65 0.15307112583172255 1116 66-67 0.13285208563392104 1116 68-69 0.16114746608706554 1116 70-71 0.1501521091263669 1116 72-73 0.09833397675407696 1116 74-75 0.1600125498431595 1116 76 0.2905516627612954 2118 1 -0.12753170301863292 2118 2 0.019110198935720746 2118 3 0.01608447276708347 2118 4 -0.016893957013458305 2118 5 0.012498084077584792 2118 6 0.0774659015506245 2118 7 0.06716297987120612 2118 8 0.0199165100823393 2118 9 0.07091905405791721 2118 10-11 0.14506014081216279 2118 12-13 0.21535084675201688 2118 14-15 0.1767948723650079 2118 16-17 0.09059974649762381 2118 18-19 0.07703886143221439 2118 20-21 0.17544867230914463 2118 22-23 0.11415582260432444 2118 24-25 0.07350680163747114 2118 26-27 0.05859951631448723 2118 28-29 0.083177644677626 2118 30-31 0.09364756210954539 2118 32-33 0.1371291986526444 2118 34-35 0.16214485773378584 2118 36-37 0.10135869027246969 2118 38-39 0.03996729158773604 2118 40-41 0.11641272696965643 2118 42-43 0.15370771457308052 2118 44-45 0.21319949724573206 2118 46-47 0.09327069666019128 2118 48-49 0.11655962058021174 2118 50-51 0.20408595530238216 2118 52-53 0.2181572381670378 2118 54-55 0.15538991221644238 2118 56-57 0.10446775267400454 2118 58-59 0.10504000191138374 2118 60-61 0.1349355180351708 2118 62-63 0.13502440487893352 2118 64-65 0.13948822161580665 2118 66-67 0.16519126773480508 2118 68-69 0.18203060648932023 2118 70-71 0.1370914062020674 2118 72-73 0.1644285398921781 2118 74-75 0.22883982603862307 2118 76 0.24151734510977008 1119 1 -0.06327846835420559 1119 2 0.014759071069512686 1119 3 0.025667185541294657 1119 4 0.006373170418356722 1119 5 -0.0049860140948680964 1119 6 0.09307649294347442 1119 7 0.07570129164011519 1119 8 0.05066932586936446 1119 9 0.12169137867239499 1119 10-11 0.21091377806433087 1119 12-13 0.25283060101060073 1119 14-15 0.1801776177185772 1119 16-17 0.04078114368648755 1119 18-19 0.04726652906074946 1119 20-21 0.0930829263970736 1119 22-23 0.09108558694699553 1119 24-25 0.059014457785657726 1119 26-27 0.10224373037635814 1119 28-29 0.05374175231360567 1119 30-31 0.1123166373213138 1119 32-33 0.15161673317124524 1119 34-35 0.17948153432939762 1119 36-37 0.06968050447706986 1119 38-39 0.0021987834118704086 1119 40-41 0.0849508060288855 1119 42-43 0.16110598166173418 1119 44-45 0.17271371580665829 1119 46-47 0.05479742724444492 1119 48-49 0.06843485328989374 1119 50-51 0.12379373013251183 1119 52-53 0.17114837192855958 1119 54-55 0.09095683176094838 1119 56-57 0.0606905256424497 1119 58-59 0.10119569631704195 1119 60-61 0.1346395307256003 1119 62-63 0.11320721383981436 1119 64-65 0.14069332804169932 1119 66-67 0.14907899307532801 1119 68-69 0.08679818770499281 1119 70-71 0.08712929909356859 1119 72-73 0.10852002194251043 1119 74-75 0.15754326471186175 1119 76 0.014505414860614962 2119 1 -0.0024736746282769673 2119 2 0.010775862258583402 2119 3 -0.010323250983219623 2119 4 0.00956467582464171 2119 5 0.030139900487398563 2119 6 0.0663406622011351 2119 7 0.03488278884802298 2119 8 0.0477117640335436 2119 9 0.12885839725020531 2119 10-11 0.1831272508472921 2119 12-13 0.23870071099250367 2119 14-15 0.1699804314608926 2119 16-17 0.015218646586852458 2119 18-19 0.035698281500927465 2119 20-21 0.13582770767075658 2119 22-23 0.07271763290427202 2119 24-25 -0.04266201212013243 2119 26-27 0.053966886516633394 2119 28-29 0.03424190119909554 2119 30-31 0.10155462400282289 2119 32-33 0.08688818814982113 2119 34-35 0.14141354586898558 2119 36-37 0.07364617573792032 2119 38-39 0.027012367182194907 2119 40-41 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-0.08203533444562794 2104 42-43 -0.0023697641686695192 2104 44-45 0.032326999809477286 2104 46-47 0.023829903166458166 2104 48-49 -0.03186421295394126 2104 50-51 -0.0261397617631971 2104 52-53 -0.07094200862112388 2104 54-55 0.0022215134616700993 2104 56-57 0.03618999710122495 2104 58-59 0.09683831435791745 2104 60-61 -0.018655189734822386 2104 62-63 -0.05021890784212246 2104 64-65 -0.14161654393387124 2104 66-67 -0.12948924746965673 2104 68-69 0.007064660573213644 2104 70-71 -0.08967097104972765 2104 72-73 -0.06535051482047294 2104 74-75 -0.013809654010238148 2104 76 -0.30436585598739896 2111 1 0.04679991286054275 2111 2 -0.01948236930829239 2111 3 -0.0012662724148952975 2111 4 0.005932563045931261 2111 5 -0.025835417216995893 2111 6 -0.15358115496417213 2111 7 -0.011848721478081359 2111 8 -0.06021582433397299 2111 9 -0.062135690722861625 2111 10-11 -0.3046595690431104 2111 12-13 -0.45825361780089224 2111 14-15 -0.2643268424677032 2111 16-17 -0.0462054429591916 2111 18-19 -0.0374340481398292 2111 20-21 0.13539197467203223 2111 22-23 -0.06236753190385258 2111 24-25 0.03396411671258903 2111 26-27 -0.07471457016148975 2111 28-29 -0.01304125634440112 2111 30-31 -0.09176467908955033 2111 32-33 -0.0631027014345591 2111 34-35 5.386968638347867E-4 2111 36-37 0.008689916781932538 2111 38-39 0.01490555895955481 2111 40-41 -0.09028482954765593 2111 42-43 -0.010846825631979584 2111 44-45 -0.03666726936978648 2111 46-47 -0.0298693706041675 2111 48-49 -0.006312047536560783 2111 50-51 -0.10741060215136855 2111 52-53 -0.11317325341580187 2111 54-55 -0.05052011301145143 2111 56-57 -0.13436407884397283 2111 58-59 -0.22535561222290568 2111 60-61 -0.07795877374770299 2111 62-63 -0.05059329121068856 2111 64-65 -0.07624398338425209 2111 66-67 -0.07369058583061161 2111 68-69 -0.13910606586971852 2111 70-71 -0.08501077043286642 2111 72-73 -0.06008264433062038 2111 74-75 -0.0075812761513063265 2111 76 -0.14688478584776732 2110 1 0.023977306794236597 2110 2 -0.018601603022858626 2110 3 -0.02481214455593772 2110 4 -0.009756992542676812 2110 5 0.010204107114624605 2110 6 -0.10990067011869087 2110 7 -0.01903236169206224 2110 8 -0.019971791453919252 2110 9 -0.069731911898117 2110 10-11 -0.3516727678984779 2110 12-13 -0.39423935088122164 2110 14-15 -0.13642255708892748 2110 16-17 -0.05233250646737275 2110 18-19 -0.0881246568853058 2110 20-21 -0.019011883192774803 2110 22-23 -0.05057675467885758 2110 24-25 -0.0612027333034888 2110 26-27 -0.08119962513296741 2110 28-29 -0.09725514702039106 2110 30-31 -0.060092374293994055 2110 32-33 -0.17246670129038932 2110 34-35 -0.0406864289465787 2110 36-37 -0.010461598847157916 2110 38-39 -0.0018423216315426316 2110 40-41 -0.18559667732105822 2110 42-43 -0.06470197930274679 2110 44-45 -0.20115612123230875 2110 46-47 -0.10967323832425535 2110 48-49 -0.12497906665984004 2110 50-51 -0.02298234471688687 2110 52-53 -0.14657997939116996 2110 54-55 -0.09677159179327788 2110 56-57 -0.16388316438484907 2110 58-59 -0.13381467836331495 2110 60-61 -0.2332147343605797 2110 62-63 -0.2517650661700088 2110 64-65 -0.2240193321383117 2110 66-67 -0.26640147471530184 2110 68-69 -0.3085247817831558 2110 70-71 -0.2125985032439459 2110 72-73 -0.2852798108346235 2110 74-75 -0.21178743747972817 2110 76 -0.3430655916549341 2109 1 0.06489556718979372 2109 2 0.009332470867228437 2109 3 -0.021326926581231476 2109 4 -0.009384848654029554 2109 5 9.60380934749594E-4 2109 6 -0.1758801245973487 2109 7 -0.08369854219893114 2109 8 -0.07462217824853923 2109 9 -0.17341716778042837 2109 10-11 -0.35379282141551016 2109 12-13 -0.48941257194128696 2109 14-15 -0.09853246752922473 2109 16-17 -0.054391699833239215 2109 18-19 -0.04715010148396459 2109 20-21 0.04289915598817373 2109 22-23 -0.049535259029021006 2109 24-25 -0.04766250363610425 2109 26-27 -0.05276232004987946 2109 28-29 -0.13620668360606203 2109 30-31 -0.03207841035965231 2109 32-33 -0.07100915181647593 2109 34-35 -0.1420570800326857 2109 36-37 -0.06465250375873666 2109 38-39 -0.004231465107125132 2109 40-41 -0.28116662642307233 2109 42-43 -0.14583286388081973 2109 44-45 -0.2939209829362994 2109 46-47 -0.08038607224984418 2109 48-49 -0.17813997332476106 2109 50-51 -0.13473384897455531 2109 52-53 -0.5249195734550582 2109 54-55 -0.2557345784398919 2109 56-57 -0.08245479295312919 2109 58-59 -0.025199242998084515 2109 60-61 -0.13261709765180285 2109 62-63 0.014997598177338034 2109 64-65 -0.0717350601969926 2109 66-67 -0.0964212959002495 2109 68-69 0.026611527540275404 2109 70-71 -0.07908457895873511 2109 72-73 -0.27119127339054216 2109 74-75 -0.25782093482577295 2109 76 -0.4970308515748698 2108 1 -0.019859286945944632 2108 2 0.007079859599976146 2108 3 -0.007850128599073969 2108 4 0.01581342362682392 2108 5 -0.0229973448845584 2108 6 0.025876383104183276 2108 7 0.0024493260949611795 2108 8 0.015566134749001037 2108 9 -0.04358972318909338 2108 10-11 0.06671827858462365 2108 12-13 0.126943504236408 2108 14-15 0.07047447284633535 2108 16-17 -0.01656320248069676 2108 18-19 -0.00646713957146261 2108 20-21 0.07792980175838693 2108 22-23 -0.09486300537582082 2108 24-25 0.007979511549095264 2108 26-27 -0.01440089617005924 2108 28-29 -0.018640676290857527 2108 30-31 -0.16957700645253482 2108 32-33 -0.1332333940110857 2108 34-35 -0.019565320798363928 2108 36-37 -0.04053238140568993 2108 38-39 -0.011938255552166765 2108 40-41 -0.08417871761547246 2108 42-43 -0.03279374663055279 2108 44-45 0.030492505262081693 2108 46-47 0.03726418640735574 2108 48-49 -0.03515363959946427 2108 50-51 -0.004786091234123546 2108 52-53 -0.17354335543877397 2108 54-55 -0.10961967997451438 2108 56-57 -0.07965416738564102 2108 58-59 -0.03219421870203121 2108 60-61 -0.10342968247824302 2108 62-63 -0.1073562906115626 2108 64-65 -0.1168785733341835 2108 66-67 -0.12183296206398353 2108 68-69 -0.18969092953399525 2108 70-71 -0.0609119484495082 2108 72-73 -0.035862204326427616 2108 74-75 -0.05647952694035041 2108 76 -0.03140827458324935 >>END_MODULE >>Per sequence quality scores pass #Quality Count 13 1.0 14 3.0 15 20.0 16 26.0 17 34.0 18 41.0 19 42.0 20 77.0 21 233.0 22 411.0 23 663.0 24 950.0 25 1142.0 26 1599.0 27 2036.0 28 3024.0 29 4763.0 30 7568.0 31 14540.0 32 25493.0 33 36728.0 34 66058.0 35 117919.0 36 238300.0 37 2281179.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 53.266425245732016 1.7149686925807661 41.06634318639956 3.9522628752876536 2 2.265194355745045 1.9556522824981715 3.6661969067199456 92.11295645503684 3 2.3648786057049076 39.36375474962984 3.661166312860124 54.610200331805125 4 2.502452860481296 1.5055390049413988 92.42560251172914 3.5664056228481726 5 2.636280928340796 1.538790873575111 41.23427939418806 54.59064880389604 6 40.414506662861015 1.8520434557682357 4.226983249192786 53.50646663217796 7 2.663824321672583 1.9932925415202383 42.21363968817454 53.12924344863264 8 3.2413079544035535 2.716877464009847 92.07096348359705 1.9708510979895462 9 4.266050627040334 2.235331894321851 91.27645075548104 2.222166723156787 10-11 66.74060331448347 27.872825873664308 2.59430579588633 2.7922650159658917 12-13 27.70326631821182 21.8652086269333 28.320994701821363 22.110530353033518 14-15 2.6383680896230626 47.067342169577394 21.784986709955938 28.509303030843608 16-17 21.638100504843287 2.675687246909396 47.28933050288099 28.396881745366326 18-19 28.258754482045063 46.502934513084895 3.6027079579713503 21.635603046898694 20-21 22.128145326416085 1.794085232561583 54.133187339025405 21.944582101996932 22-23 27.658865083754037 2.333624703426869 40.70697682715807 29.300533385661026 24-25 2.726296448258023 21.98389139625738 27.87373566191555 47.41607649356905 26-27 3.8197905703123607 45.76586331769449 21.90679130171076 28.507554810282393 28-29 22.507412098399843 1.9859250405836915 27.873557272062367 47.6331055889541 30-31 48.857163244554656 2.389728312253599 21.02679415594841 27.72631428724334 32-33 65.48818167222649 3.5776620225841556 2.8174001462796796 28.116756158909684 34-35 2.9002622330841823 47.14893768842428 21.884242824268156 28.06655725422338 36-37 22.325472287136307 26.894268334017163 23.121768913784184 27.658490465062346 38-39 48.12719196532102 2.925308168471377 47.16574201259432 1.7817578536132865 40-41 28.936760797045864 46.7191430151453 22.457070481830993 1.8870257059778441 42-43 45.991847583709436 2.645806946500883 49.1481884510409 2.214157018748774 44-45 2.4494889130706246 4.352676739747043 90.88101396792551 2.316820379256828 46-47 3.3094350393349625 91.52107676115384 2.423907808123874 2.745580391387338 48-49 21.739747043188185 28.748595179906168 22.184098328487075 27.327559448418576 50-51 41.01833847690743 28.16916709777548 27.916870328415722 2.8956240969013685 52-53 46.11240344649196 29.625363469325865 21.121162388283356 3.141070695898817 54-55 2.920277574611556 72.42892056299837 2.6482865654601566 22.00251529692991 56-57 26.98836898157233 21.740942255204523 3.759316410082594 47.51137235314055 58-59 1.7228535240915495 22.697629198851168 53.965713470217814 21.613803806839467 60-61 2.1636370123267388 47.33715682251993 23.15630518936083 27.342900975792496 62-63 2.72356708350429 28.042474624043383 66.4092798401627 2.8246784522896338 64-65 28.370818987815973 40.8425709545641 28.099595055033273 2.687015002586653 66-67 27.767914801006118 28.23583138591077 21.737410136111457 22.258843676971654 68-69 3.3078116916709774 22.260609736518187 46.528265872237185 27.903312699573647 70-71 53.28449613785968 4.41566619690672 21.25695274452789 21.04288492070571 72-73 28.1938562534563 66.38478691332037 2.5807838450148957 2.8405729882084305 74-75 20.817953154824554 22.946821984765506 27.336193517312736 28.899031343097203 76 2.2583441853827355 90.85348841357903 2.8924844354853096 3.9956829655529194 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1.0 1 0.5 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 1.0 10 1.0 11 0.5 12 1.0 13 2.0 14 4.5 15 7.5 16 13.0 17 17.0 18 18.5 19 16.0 20 25.5 21 39.0 22 40.5 23 52.5 24 81.0 25 99.0 26 110.0 27 131.0 28 183.0 29 225.0 30 389.0 31 1112.5 32 2768.0 33 3864.0 34 7755.0 35 33704.5 36 527528.0 37 999293.0 38 537787.0 39 44735.5 40 10447.0 41 8071.0 42 8438.0 43 8129.0 44 10403.5 45 13553.0 46 14119.0 47 44271.0 48 682162.0 49 698440.0 50 106979.0 51 65103.0 52 15768.5 53 8190.5 54 8071.0 55 6158.5 56 3497.5 57 2510.0 58 2271.0 59 2552.5 60 3054.0 61 3574.0 62 3874.0 63 4171.0 64 4739.0 65 5209.5 66 5643.5 67 5878.0 68 5881.5 69 5808.5 70 5533.0 71 5334.0 72 5000.0 73 4241.0 74 3469.5 75 3123.0 76 2766.0 77 2107.0 78 1589.0 79 1373.0 80 1182.0 81 881.5 82 680.0 83 588.0 84 500.5 85 362.5 86 265.0 87 218.0 88 180.0 89 113.0 90 71.0 91 45.5 92 33.0 93 25.0 94 12.5 95 7.0 96 6.0 97 5.0 98 4.0 99 3.0 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 3.567797063703017E-5 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 2802850.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 4.307215674339774 #Duplication Level Percentage of deduplicated Percentage of total 1 87.46401445825272 3.7672637401526674 2 4.339976231312511 0.37386427299542613 3 2.5523794275424563 0.32980946031519737 4 1.703015914559547 0.2934102736336386 5 1.0502646340572561 0.226185814700807 6 0.7841417152523521 0.2026480492103164 7 0.38644321030350826 0.11651459768630165 8 0.29030701940309783 0.10003319554751072 9 0.15108289568480288 0.05856719547764016 >10 0.7935594540845229 0.6553206726739874 >50 0.15769007433861587 0.4854059182550572 >100 0.22174755835966953 2.298187365203477 >500 0.03982768132882964 1.1787382923322527 >1k 0.05393331846585946 4.447090592724361 >5k 0.0066379468881057795 2.1221363489217477 >10k+ 0.004978460166079335 83.34482421016959 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GCCTCCCTTAGGTCACTAGTTGCCTCATCCGGCACACTGGAGTTTAACAA 1312212 46.81706120555863 No Hit TCATTGTTTGGCATAGTCACGTTCAATGCTGGGTATGTGTAGTTTAAGTG 960569 34.27115257684143 No Hit CTCCCTTAGGTCACTAGTTGCCTCATCCGGCACACTGGAGTTTAACAATG 21909 0.7816686586866939 No Hit CATTGTTTGGCATAGTCACGTTCAATGCTGGGTATGTGTAGTTTAAGTGG 13425 0.47897675580212995 No Hit GCCTCCCTTAGGTCACTAGTTGCCTCATCCGGCACCCTGGAGTTTAACAA 12577 0.44872183670192844 No Hit ATTGTTTGGCATAGTCACGTTCAATGCTGGGTATGTGTAGTTTAAGTGGG 11360 0.40530174643666267 No Hit CCTCCCTTAGGTCACTAGTTGCCTCATCCGGCACACTGGAGTTTAACAAT 9830 0.3507144513620065 No Hit TGTTTGGCATAGTCACGTTCAATGCTGGGTATGTGTAGTTTAAGTGGGTC 8177 0.29173876589899567 No Hit TCCCTTAGGTCACTAGTTGCCTCATCCGGCACACTGGAGTTTAACAATGA 7823 0.27910876429348697 No Hit TTGTTTGGCATAGTCACGTTCAATGCTGGGTATGTGTAGTTTAAGTGGGT 7428 0.26501596589186005 No Hit GCCTCCCTTAGGTCACTAGTTGCCTCATCCGGCACACTGGAGTTTAACAC 7253 0.2587723210303798 No Hit CCCTTAGGTCACTAGTTGCCTCATCCGGCACACTGGAGTTTAACAATGAA 6682 0.23840019979663557 No Hit TTGGCATAGTCACGTTCAATGCTGGGTATGTGTAGTTTAAGTGGGTCAAC 6534 0.2331198601423551 No Hit TGGCATAGTCACGTTCAATGCTGGGTATGTGTAGTTTAAGTGGGTCAACC 5652 0.20165189004049452 No Hit GTTTGGCATAGTCACGTTCAATGCTGGGTATGTGTAGTTTAAGTGGGTCA 4551 0.1623704443691243 No Hit TCATTGTTTGGCATAGTCACGTTCAGTGCTGGATATTTGTATTTTAATTG 4355 0.15537756212426637 No Hit GCCTCCCTTAGGTCACTAGTTGAACGAAGCAAAGCCTACAGCAACTGTTA 4213 0.15031129029380808 No Hit TCATTGTTTGGCATAGTCACGTCCAATGCTGGGTATGTGTAGTTTAAGTG 3905 0.1393224753376028 No Hit GCCTCCCTTAGGTCACTAGTTGCCTCATCAGGCACACTGGAGTTTAACAA 3273 0.11677399789499973 No Hit TTTGGCATAGTCACGTTCAATGCTGGGTATGTGTAGTTTAAGTGGGTCAA 2864 0.10218170790445438 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0015341527373922972 0.0 0.0 2 0.0015341527373922972 0.0 0.0 3 0.001748220561214478 0.0 0.0 4 0.001748220561214478 0.0 0.0 5 0.001748220561214478 0.0 0.0 6 0.001748220561214478 0.0 0.0 7 0.001748220561214478 0.0 0.0 8 0.001748220561214478 0.0 0.0 9 0.001748220561214478 0.0 0.0 10 0.0017838985318515083 0.0 0.0 11 0.0017838985318515083 0.0 0.0 12 0.0017838985318515083 0.0 0.0 13 0.0018195765024885385 0.0 0.0 14 0.0018552544731255686 0.0 0.0 15 0.0018552544731255686 0.0 0.0 16 0.0018552544731255686 0.0 0.0 17 0.0018552544731255686 0.0 0.0 18 0.0018552544731255686 0.0 0.0 19 0.0018552544731255686 0.0 0.0 20 0.0018552544731255686 0.0 0.0 21 0.0018552544731255686 0.0 0.0 22 0.0018552544731255686 0.0 0.0 23 0.0018552544731255686 0.0 0.0 24 0.0018552544731255686 0.0 0.0 25 0.0018552544731255686 0.0 0.0 26 0.0018552544731255686 0.0 0.0 27 0.0018909324437625988 0.0 0.0 28 0.001962288385036659 0.0 0.0 29 0.00210500026758478 0.0 0.0 30 0.00214067823822181 0.0 0.0 31 0.0022120341794958703 0.0 0.0 32 0.0022477121501329003 0.0 0.0 33 0.0022833901207699307 0.0 0.0 34 0.0022833901207699307 0.0 0.0 35 0.0024617799739550814 0.0 0.0 36 0.0026758477977772625 0.0 0.0 37 0.0027115257684142925 0.0 0.0 38 0.0029255935922364736 0.0 0.0 39 0.0031396614160586547 0.0 0.0 40 0.0033537292398808354 0.0 0.0 41 0.003639153004977077 0.0 0.0 42 0.003853220828799258 0.0 0.0 43 0.004031610681984409 0.0 0.0 44 0.00431703444708065 0.0 0.0 45 0.0044954243002658005 0.0 0.0 46 0.004638136182813921 0.0 0.0 47 0.004780848065362042 0.0 0.0 48 0.005173305742369374 0.0 0.0 49 0.005494407478102646 0.0 0.0 50 0.0059582210963840376 0.0 0.0 51 0.006350678773391369 0.0 0.0 52 0.00656474659721355 0.0 0.0 53 0.006778814421035732 0.0 0.0 54 0.007385339921865244 0.0 0.0 55 0.007777797598872576 0.0 0.0 56 0.008348645129065059 0.0 0.0 57 0.009133560483079723 0.0 0.0 58 0.009704408013272206 0.0 0.0 59 0.010203899602190628 0.0 0.0 60 0.01081042510302014 0.0 0.0 61 0.011416950603849653 0.0 0.0 62 0.011987798134042136 0.0 0.0 63 0.0127727134880568 0.0 0.0 64 0.013379238988886313 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTCTATT 180 0.0 70.00001 58 TTAGGTT 35 3.7161954E-9 70.00001 8 TAATATA 85 0.0 70.00001 63 TCATTAT 25 2.84206E-6 70.00001 1 TAGACAC 25 2.84206E-6 70.00001 14 TTAGACT 95 0.0 70.00001 63 TTACTAG 35 3.7161954E-9 70.00001 13 TAGCCTA 90 0.0 70.00001 40 AATATAC 90 0.0 70.00001 64 TATCTTA 25 2.84206E-6 70.00001 40 GGTATAT 50 0.0 70.00001 32 ACTAATG 70 0.0 70.00001 69 GTATCTT 35 3.7161954E-9 70.00001 39 CTACCTT 25 2.84206E-6 70.00001 3 GCCTAAG 200 0.0 70.00001 42 GTATATG 50 0.0 70.00001 33 CAATGTA 70 0.0 70.00001 48 TACTAAT 70 0.0 70.00001 68 ACACTCG 25 2.84206E-6 70.00001 34 ATAGTAC 25 2.84206E-6 70.00001 13 >>END_MODULE