FastQCFastQC Report
Tue 25 Aug 2015
000000000-AHV5L l01n01 kokuya7.34100000033d3e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AHV5L l01n01 kokuya7.34100000033d3e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2802850
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTCCCTTAGGTCACTAGTTGCCTCATCCGGCACACTGGAGTTTAACAA131221246.81706120555863No Hit
TCATTGTTTGGCATAGTCACGTTCAATGCTGGGTATGTGTAGTTTAAGTG96056934.27115257684143No Hit
CTCCCTTAGGTCACTAGTTGCCTCATCCGGCACACTGGAGTTTAACAATG219090.7816686586866939No Hit
CATTGTTTGGCATAGTCACGTTCAATGCTGGGTATGTGTAGTTTAAGTGG134250.47897675580212995No Hit
GCCTCCCTTAGGTCACTAGTTGCCTCATCCGGCACCCTGGAGTTTAACAA125770.44872183670192844No Hit
ATTGTTTGGCATAGTCACGTTCAATGCTGGGTATGTGTAGTTTAAGTGGG113600.40530174643666267No Hit
CCTCCCTTAGGTCACTAGTTGCCTCATCCGGCACACTGGAGTTTAACAAT98300.3507144513620065No Hit
TGTTTGGCATAGTCACGTTCAATGCTGGGTATGTGTAGTTTAAGTGGGTC81770.29173876589899567No Hit
TCCCTTAGGTCACTAGTTGCCTCATCCGGCACACTGGAGTTTAACAATGA78230.27910876429348697No Hit
TTGTTTGGCATAGTCACGTTCAATGCTGGGTATGTGTAGTTTAAGTGGGT74280.26501596589186005No Hit
GCCTCCCTTAGGTCACTAGTTGCCTCATCCGGCACACTGGAGTTTAACAC72530.2587723210303798No Hit
CCCTTAGGTCACTAGTTGCCTCATCCGGCACACTGGAGTTTAACAATGAA66820.23840019979663557No Hit
TTGGCATAGTCACGTTCAATGCTGGGTATGTGTAGTTTAAGTGGGTCAAC65340.2331198601423551No Hit
TGGCATAGTCACGTTCAATGCTGGGTATGTGTAGTTTAAGTGGGTCAACC56520.20165189004049452No Hit
GTTTGGCATAGTCACGTTCAATGCTGGGTATGTGTAGTTTAAGTGGGTCA45510.1623704443691243No Hit
TCATTGTTTGGCATAGTCACGTTCAGTGCTGGATATTTGTATTTTAATTG43550.15537756212426637No Hit
GCCTCCCTTAGGTCACTAGTTGAACGAAGCAAAGCCTACAGCAACTGTTA42130.15031129029380808No Hit
TCATTGTTTGGCATAGTCACGTCCAATGCTGGGTATGTGTAGTTTAAGTG39050.1393224753376028No Hit
GCCTCCCTTAGGTCACTAGTTGCCTCATCAGGCACACTGGAGTTTAACAA32730.11677399789499973No Hit
TTTGGCATAGTCACGTTCAATGCTGGGTATGTGTAGTTTAAGTGGGTCAA28640.10218170790445438No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCTATT1800.070.0000158
TTAGGTT353.7161954E-970.000018
TAATATA850.070.0000163
TCATTAT252.84206E-670.000011
TAGACAC252.84206E-670.0000114
TTAGACT950.070.0000163
TTACTAG353.7161954E-970.0000113
TAGCCTA900.070.0000140
AATATAC900.070.0000164
TATCTTA252.84206E-670.0000140
GGTATAT500.070.0000132
ACTAATG700.070.0000169
GTATCTT353.7161954E-970.0000139
CTACCTT252.84206E-670.000013
GCCTAAG2000.070.0000142
GTATATG500.070.0000133
CAATGTA700.070.0000148
TACTAAT700.070.0000168
ACACTCG252.84206E-670.0000134
ATAGTAC252.84206E-670.0000113
Produced by FastQC (version 0.11.2)