##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename 000000000-AHV5L l01n01 kokuya6.34100000033d21.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2099455 Sequences flagged as poor quality 0 Sequence length 76 %GC 51 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 33.40345375347412 34.0 34.0 34.0 34.0 34.0 2 33.6667320804685 34.0 34.0 34.0 34.0 34.0 3 33.699275288110485 34.0 34.0 34.0 34.0 34.0 4 33.744169796447174 34.0 34.0 34.0 34.0 34.0 5 33.85296088746841 34.0 34.0 34.0 34.0 34.0 6 37.66773091111741 38.0 38.0 38.0 37.0 38.0 7 37.68228468816907 38.0 38.0 38.0 37.0 38.0 8 37.5797985667709 38.0 38.0 38.0 37.0 38.0 9 37.530781559976276 38.0 38.0 38.0 37.0 38.0 10-11 37.37747367769254 38.0 38.0 38.0 36.5 38.0 12-13 37.46461748406134 38.0 38.0 38.0 37.0 38.0 14-15 37.43500860937719 38.0 38.0 38.0 37.0 38.0 16-17 37.61248300154088 38.0 38.0 38.0 37.0 38.0 18-19 37.57522095019898 38.0 38.0 38.0 37.0 38.0 20-21 37.44625938636456 38.0 38.0 38.0 37.0 38.0 22-23 37.510827571917474 38.0 38.0 38.0 37.0 38.0 24-25 37.57345739727691 38.0 38.0 38.0 37.0 38.0 26-27 36.85549535474683 38.0 38.0 38.0 35.5 38.0 28-29 37.21437158691184 38.0 38.0 38.0 36.5 38.0 30-31 37.00239538356383 38.0 38.0 38.0 36.0 38.0 32-33 37.32305241122101 38.0 38.0 38.0 37.0 38.0 34-35 37.417553841354064 38.0 38.0 38.0 37.0 38.0 36-37 36.83005637177267 38.0 38.0 38.0 35.5 38.0 38-39 37.365065695621006 38.0 38.0 38.0 37.0 38.0 40-41 36.77133851404293 38.0 37.5 38.0 35.5 38.0 42-43 36.89427946776664 38.0 38.0 38.0 36.0 38.0 44-45 36.92343298617975 38.0 38.0 38.0 36.0 38.0 46-47 36.34932542016857 38.0 37.5 38.0 34.5 38.0 48-49 37.11728615283491 38.0 37.5 38.0 36.5 38.0 50-51 36.85830965655373 38.0 38.0 38.0 35.0 38.0 52-53 37.03905061075375 38.0 38.0 38.0 36.5 38.0 54-55 36.54754019495536 38.0 38.0 38.0 34.0 38.0 56-57 36.63341486242858 38.0 37.5 38.0 34.5 38.0 58-59 37.05831751573623 38.0 38.0 38.0 36.0 38.0 60-61 36.972050365451985 38.0 37.5 38.0 36.0 38.0 62-63 36.707175909938535 38.0 37.5 38.0 35.0 38.0 64-65 37.03554589167189 38.0 38.0 38.0 36.0 38.0 66-67 37.18440690560169 38.0 38.0 38.0 37.0 38.0 68-69 37.26411568716644 38.0 38.0 38.0 37.0 38.0 70-71 37.223273897273344 38.0 38.0 38.0 37.0 38.0 72-73 36.58541573884651 38.0 37.5 38.0 35.0 38.0 74-75 36.755317451433825 38.0 38.0 38.0 35.0 38.0 76 36.057275340505036 38.0 37.0 38.0 33.0 38.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.041123041275326955 1101 2 0.005157788636253713 1101 3 0.08117547522325452 1101 4 -2.1033447647056391E-4 1101 5 0.019097652513067942 1101 6 -0.04328292855122129 1101 7 -0.1036451215345977 1101 8 -0.21851734090493835 1101 9 -0.5173935303620496 1101 10-11 -0.7048865768813641 1101 12-13 -0.9637269480895156 1101 14-15 -0.3613726687121499 1101 16-17 -0.10517092150206508 1101 18-19 0.029049410812341137 1101 20-21 0.16372782909446215 1101 22-23 0.10512627527644725 1101 24-25 0.08635620066495164 1101 26-27 0.09418489894954973 1101 28-29 0.12052010448451966 1101 30-31 0.16622975589525169 1101 32-33 0.10300347186875314 1101 34-35 0.09284328125947638 1101 36-37 0.060423806594201324 1101 38-39 0.03872417365838032 1101 40-41 0.1745805840906982 1101 42-43 0.09703759759189268 1101 44-45 0.09981519000356798 1101 46-47 0.37677209941407597 1101 48-49 0.0984997331104438 1101 50-51 0.1892388944989989 1101 52-53 0.21150648560839613 1101 54-55 0.3636207713727089 1101 56-57 0.2722153111532819 1101 58-59 0.17932685621166655 1101 60-61 0.17320465470816515 1101 62-63 0.11723091871385094 1101 64-65 0.1570672294085682 1101 66-67 0.16262513105765208 1101 68-69 0.0412307338751603 1101 70-71 0.028015896786747874 1101 72-73 0.23938479499346244 1101 74-75 0.16646683330913703 1101 76 0.09401646587487278 1102 1 0.047135767104009574 1102 2 0.029607691611886366 1102 3 0.08536562051634888 1102 4 0.03836522042155366 1102 5 0.030754469194384626 1102 6 0.020883668029995306 1102 7 -0.00500635693624929 1102 8 -0.10451409733887118 1102 9 -0.29743960629745203 1102 10-11 -0.30987012921612234 1102 12-13 -0.028943821987795104 1102 14-15 0.14402404161860005 1102 16-17 0.041546219174925625 1102 18-19 0.07738395914933704 1102 20-21 0.13447739045032847 1102 22-23 0.12791632775030592 1102 24-25 0.05457413929461552 1102 26-27 0.050777465714880066 1102 28-29 0.08767615487931124 1102 30-31 0.12919852116437625 1102 32-33 0.12781697362962774 1102 34-35 0.1482936537792625 1102 36-37 0.09536219892813591 1102 38-39 0.05106136969322961 1102 40-41 0.1745566472132296 1102 42-43 0.11430139798489591 1102 44-45 0.1512786763910441 1102 46-47 0.39494698230895153 1102 48-49 0.09324723991269934 1102 50-51 0.16690047608305747 1102 52-53 0.22644500392590317 1102 54-55 0.3090831497858062 1102 56-57 0.3033433475700278 1102 58-59 0.19630460581149833 1102 60-61 0.18092162479660345 1102 62-63 0.14394532520697112 1102 64-65 0.14460903807630388 1102 66-67 0.22879786269801627 1102 68-69 0.08268315754359179 1102 70-71 0.1178472524618499 1102 72-73 0.35999752798219475 1102 74-75 0.29740863537124795 1102 76 0.5418988013105448 1103 1 0.017487522550311496 1103 2 0.0631132248798778 1103 3 0.025080538532748164 1103 4 0.008822073713631085 1103 5 -5.7485048877481404E-5 1103 6 0.053249051013274595 1103 7 0.03965183079660761 1103 8 0.06091591453937184 1103 9 0.0996411856677284 1103 10-11 0.22356595758823516 1103 12-13 0.21165771033810188 1103 14-15 0.18754553143903507 1103 16-17 0.09443998027673217 1103 18-19 0.057646833531350694 1103 20-21 0.17592870599847288 1103 22-23 0.09789893959103324 1103 24-25 0.04495782519438052 1103 26-27 0.10505056231101406 1103 28-29 -0.017230509281752404 1103 30-31 0.01740765317830295 1103 32-33 0.05891315699910393 1103 34-35 0.11264012039940496 1103 36-37 -0.045751575232308994 1103 38-39 0.08733279096932733 1103 40-41 0.06079192440680714 1103 42-43 -0.003990165576119864 1103 44-45 -0.08966562966165981 1103 46-47 0.1265933731989648 1103 48-49 -0.141063254723079 1103 50-51 0.0883835027983153 1103 52-53 -0.010669025189642412 1103 54-55 0.21203739853189063 1103 56-57 0.2766622868819013 1103 58-59 0.20447437592374484 1103 60-61 0.09369679708385803 1103 62-63 0.13323280483711386 1103 64-65 0.19888933434327782 1103 66-67 0.16728557513254572 1103 68-69 0.08295037809799766 1103 70-71 0.1949121475538007 1103 72-73 0.3210106857883588 1103 74-75 0.25146720606414164 1103 76 0.546323597573739 2116 1 -0.022829874726667754 2116 2 0.06393321815032493 2116 3 -0.03441743244253814 2116 4 -0.00565120556475307 2116 5 -0.025590289435214686 2116 6 0.019964246928502405 2116 7 0.051636331850772876 2116 8 -0.005413822234480392 2116 9 0.02722961868828122 2116 10-11 0.23208895518042283 2116 12-13 0.14587943548002613 2116 14-15 0.13593597187510653 2116 16-17 0.032176859770018496 2116 18-19 0.08296224797530272 2116 20-21 0.17040657571617857 2116 22-23 0.13324535698020412 2116 24-25 0.05556034856531511 2116 26-27 0.17052929213900825 2116 28-29 0.0821069853068579 2116 30-31 0.2014591405869055 2116 32-33 0.036231257033499276 2116 34-35 0.1337338328924531 2116 36-37 0.08597615543441606 2116 38-39 0.11773185216875959 2116 40-41 0.13997951218009774 2116 42-43 0.09905711494509006 2116 44-45 0.036379104588355915 2116 46-47 0.281371291226705 2116 48-49 0.11453406468465488 2116 50-51 0.17859740404834668 2116 52-53 0.24438133205432422 2116 54-55 0.3784577845407 2116 56-57 0.3037513478082303 2116 58-59 0.21712766127403427 2116 60-61 0.1849081202875098 2116 62-63 0.1512249074954184 2116 64-65 0.20893278083349287 2116 66-67 0.22203199835755072 2116 68-69 -0.02463969629363305 2116 70-71 0.19349422377468528 2116 72-73 0.365787960351561 2116 74-75 0.236943044974538 2116 76 0.4069528477409463 1117 1 -0.03414665781169646 1117 2 -0.07107097524495032 1117 3 -0.03388905491135574 1117 4 0.01765895895862002 1117 5 0.027125330003471504 1117 6 0.072841113392343 1117 7 0.07142695526331266 1117 8 0.048215125087139654 1117 9 0.09139627794448302 1117 10-11 0.17370587796192183 1117 12-13 0.18638484798896116 1117 14-15 0.17236802122544503 1117 16-17 0.12042954156996188 1117 18-19 -0.013278570462077255 1117 20-21 0.14602138470966963 1117 22-23 0.05093964054042743 1117 24-25 -0.06389634205381611 1117 26-27 0.1404021682079204 1117 28-29 0.1812826941268284 1117 30-31 -0.00843302398871515 1117 32-33 0.12015442841859425 1117 34-35 0.20474103766797924 1117 36-37 0.18206444571851677 1117 38-39 0.07357955438823183 1117 40-41 0.25947590666604725 1117 42-43 -0.2945161316376641 1117 44-45 -0.02214486404204763 1117 46-47 -0.2998154049002508 1117 48-49 -0.021081752363258488 1117 50-51 -0.2284723435920668 1117 52-53 -0.05016557283646961 1117 54-55 -0.10764063422414694 1117 56-57 0.21490574798318818 1117 58-59 0.12186146334688885 1117 60-61 0.18815824478327414 1117 62-63 0.13244496112308468 1117 64-65 0.19966920523732767 1117 66-67 0.1902633644262295 1117 68-69 0.2491739222924778 1117 70-71 0.10921345706417895 1117 72-73 0.27792429307871913 1117 74-75 0.3562934244087259 1117 76 0.580138891654606 2117 1 -0.0131089707211558 2117 2 -0.01681802770634988 2117 3 -0.0023830288637327612 2117 4 0.061114662790842544 2117 5 0.02498304824525377 2117 6 -0.01662668196708239 2117 7 0.02946913759667069 2117 8 -0.035107305780194054 2117 9 -0.22920881948061123 2117 10-11 -0.46831114044717026 2117 12-13 -0.943150354888445 2117 14-15 -1.4164918354957265 2117 16-17 -0.1508426424251752 2117 18-19 0.06832448635937283 2117 20-21 0.20142173171087308 2117 22-23 0.14365203442622487 2117 24-25 0.014998918929393312 2117 26-27 0.16262952088732874 2117 28-29 0.16823286481085375 2117 30-31 0.13359378199480432 2117 32-33 0.13396015422005547 2117 34-35 0.22867706143418332 2117 36-37 0.2767819016814741 2117 38-39 0.12561698939592247 2117 40-41 0.3087379129920578 2117 42-43 0.24676592090823135 2117 44-45 0.2958459476083988 2117 46-47 0.08575098583661855 2117 48-49 0.276376910977973 2117 50-51 0.30428856920134706 2117 52-53 0.3263182656953063 2117 54-55 0.4782625652564718 2117 56-57 0.20556961662408213 2117 58-59 0.25173185544134213 2117 60-61 0.2636477291259709 2117 62-63 0.338153423079703 2117 64-65 0.25134312691420035 2117 66-67 0.2686330889940294 2117 68-69 0.30358546022319643 2117 70-71 0.21528405927462302 2117 72-73 0.26213385490520835 2117 74-75 0.24770113110258052 2117 76 0.3951628184012961 1116 1 0.1387551325261711 1116 2 0.021778545167450147 1116 3 0.02615596802399267 1116 4 0.07382466362887641 1116 5 -0.005076028855903303 1116 6 0.0043194019333583356 1116 7 0.05951922200377879 1116 8 -0.1503423466348437 1116 9 -0.3066815435373442 1116 10-11 -0.32404588116627053 1116 12-13 -0.04434676809818683 1116 14-15 0.16183192283454417 1116 16-17 0.0530851353943973 1116 18-19 0.050240581024795006 1116 20-21 0.2061308941638842 1116 22-23 0.1387190693617839 1116 24-25 0.09550972144685232 1116 26-27 0.05380044488313729 1116 28-29 0.07922844526914474 1116 30-31 0.1489307692078583 1116 32-33 0.06107806051092979 1116 34-35 0.10929133241974398 1116 36-37 0.07336411808663001 1116 38-39 0.036139718605085136 1116 40-41 0.1547815212598067 1116 42-43 0.11550505947462142 1116 44-45 0.19419806266729722 1116 46-47 0.08394289447644354 1116 48-49 0.12390511855734587 1116 50-51 0.15153637618244176 1116 52-53 0.14326120110310114 1116 54-55 0.3256160101493748 1116 56-57 0.3210411443375918 1116 58-59 0.15543282142114379 1116 60-61 0.15433296016560405 1116 62-63 -0.04186538809663887 1116 64-65 0.04333060152602286 1116 66-67 0.14115472263412698 1116 68-69 0.20132316783152504 1116 70-71 0.1653577416306291 1116 72-73 0.1707953588790474 1116 74-75 0.16896005228372246 1116 76 0.27728368055922203 2118 1 -0.14863639946270268 2118 2 0.056530808993706216 2118 3 0.03256423147725229 2118 4 -0.05499326107941016 2118 5 0.017344595886996217 2118 6 0.07744033449567667 2118 7 0.06711677136360805 2118 8 -0.05711717401316463 2118 9 0.12487322300135162 2118 10-11 0.2224780627167462 2118 12-13 0.2303982552221555 2118 14-15 0.21714222097853764 2118 16-17 0.12571059801425122 2118 18-19 0.11186589628519528 2118 20-21 0.22521294616462484 2118 22-23 0.12997109246200722 2118 24-25 0.09834619324321636 2118 26-27 0.11226079600128713 2118 28-29 0.14245107646431876 2118 30-31 0.11955578524852939 2118 32-33 0.1539361744591261 2118 34-35 0.1454668912904964 2118 36-37 0.11047621169331023 2118 38-39 0.09262088701947135 2118 40-41 0.1843846392194095 2118 42-43 0.25602265085646536 2118 44-45 0.3280952328471187 2118 46-47 0.1948736877255044 2118 48-49 0.22210672174154666 2118 50-51 0.27368674115733427 2118 52-53 0.2818429346267095 2118 54-55 0.2822336157517924 2118 56-57 0.25791450808169003 2118 58-59 0.18023614088078688 2118 60-61 0.1648354902369462 2118 62-63 0.24964370491575494 2118 64-65 0.20068441341325638 2118 66-67 0.275473803284342 2118 68-69 0.28803238332164227 2118 70-71 0.2570548459195763 2118 72-73 0.35123698438740547 2118 74-75 0.31464779916189656 2118 76 0.4103891845290448 1119 1 -0.1512786102476582 1119 2 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60-61 0.1940208509983563 1119 62-63 0.19131395542883212 1119 64-65 0.18219914259610448 1119 66-67 0.15057679556836945 1119 68-69 -0.00476845346873489 1119 70-71 0.15960719421179448 1119 72-73 0.3214617455621678 1119 74-75 0.23438973087362314 1119 76 0.1696435323179486 2119 1 0.025783378238088517 2119 2 0.030039200525393994 2119 3 0.012716660251115286 2119 4 0.01899202689477164 2119 5 0.036966589313102816 2119 6 0.11077542482487956 2119 7 0.08382808790108953 2119 8 0.09098246436979451 2119 9 0.16673707833815854 2119 10-11 0.24035186230901928 2119 12-13 0.25248509209907866 2119 14-15 0.2310275546516678 2119 16-17 0.055171017758851804 2119 18-19 0.0713545524886996 2119 20-21 0.1602401770419064 2119 22-23 0.09621672833160488 2119 24-25 -0.05499584319004924 2119 26-27 0.036184478241601425 2119 28-29 0.06909463948817773 2119 30-31 0.060507367389199374 2119 32-33 0.07915211863373628 2119 34-35 0.160399855074715 2119 36-37 0.02748710455596637 2119 38-39 0.022555218019419954 2119 40-41 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2104 54-55 -0.2022353843419893 2104 56-57 -0.06703536239958652 2104 58-59 0.07097918070936515 2104 60-61 -0.023600023242266843 2104 62-63 -0.17328937104635145 2104 64-65 -0.20618010340734827 2104 66-67 -0.17653979297601552 2104 68-69 0.012110512130554696 2104 70-71 -0.16018922157531534 2104 72-73 -0.25586720474864677 2104 74-75 -0.09067996340413487 2104 76 -0.4891577624819874 2111 1 0.07385992559742505 2111 2 0.03884153073519059 2111 3 0.02028708578355065 2111 4 0.03822485087977867 2111 5 -0.03205201329063101 2111 6 -0.17052428487171056 2111 7 -0.05925006865197702 2111 8 -0.011060036179443955 2111 9 -0.08484029631700452 2111 10-11 -0.40721217508912844 2111 12-13 -0.5237490216729412 2111 14-15 -0.38967770185867323 2111 16-17 -0.07710373362679235 2111 18-19 -0.08532789805798302 2111 20-21 0.15046600731695747 2111 22-23 -0.023578219043301374 2111 24-25 0.025923902224960216 2111 26-27 -0.08245557876520593 2111 28-29 -0.026511167228598254 2111 30-31 -0.09560712289362527 2111 32-33 -0.1200319350265957 2111 34-35 -0.0036038999808667427 2111 36-37 0.011015608362505702 2111 38-39 0.03200819754090389 2111 40-41 -0.15887639772429907 2111 42-43 -0.0026409377760430175 2111 44-45 0.048591543958451666 2111 46-47 0.028962629461226186 2111 48-49 0.010661329734176661 2111 50-51 -0.03916903624714507 2111 52-53 -0.15799501929912196 2111 54-55 -0.029196156848783517 2111 56-57 -0.4073269431315012 2111 58-59 -0.35867898670121434 2111 60-61 -0.07553065266327508 2111 62-63 -0.0508132316660479 2111 64-65 -0.07548479974655464 2111 66-67 -0.12669231486488286 2111 68-69 -0.14589895324704827 2111 70-71 -0.09529808065931888 2111 72-73 -0.08493430162826598 2111 74-75 0.012425431684782495 2111 76 -0.3108490402953663 2110 1 0.030852487057941858 2110 2 -0.0675950286895386 2110 3 -0.018028234239579888 2110 4 -0.005517185226914023 2110 5 0.033525083388092014 2110 6 -0.11931881921071863 2110 7 -0.043481714702814145 2110 8 -0.02974399545879436 2110 9 -0.13346341733350897 2110 10-11 -0.4010466356990463 2110 12-13 -0.44392672561981783 2110 14-15 -0.17588738087584943 2110 16-17 -0.09777346532176523 2110 18-19 -0.11076739042440664 2110 20-21 -0.07307990495359462 2110 22-23 -0.09706428989207438 2110 24-25 -0.10192760498634357 2110 26-27 0.0028730568256136735 2110 28-29 -0.07077199409897617 2110 30-31 -0.10955390458295255 2110 32-33 -0.08306281481004163 2110 34-35 -0.008688811026729581 2110 36-37 -0.05017945857445483 2110 38-39 0.027427852150950116 2110 40-41 -0.17799941596091884 2110 42-43 0.09344938655022617 2110 44-45 -0.09203897039915176 2110 46-47 0.06051726192406903 2110 48-49 -0.08531827403078296 2110 50-51 0.02881843451321231 2110 52-53 -0.08454075758685065 2110 54-55 -0.09378105613068755 2110 56-57 -0.20608931231932814 2110 58-59 -0.10707105419911755 2110 60-61 -0.17978244295267842 2110 62-63 -0.34612597352384 2110 64-65 -0.2708586213380073 2110 66-67 -0.4204225715297838 2110 68-69 -0.4192652483788706 2110 70-71 -0.2702732060693549 2110 72-73 -0.4283686481896609 2110 74-75 -0.22907075555293233 2110 76 -0.35801776400445817 2109 1 0.043000220997058136 2109 2 -0.031446021612389075 2109 3 -0.019779111082200984 2109 4 -0.05646323912309015 2109 5 -0.018940061337744396 2109 6 -0.18107693546569692 2109 7 -0.09159547333081974 2109 8 -0.07469138490173322 2109 9 -0.2588269481696628 2109 10-11 -0.4626166208457647 2109 12-13 -0.5486944403370444 2109 14-15 -0.15557349618221394 2109 16-17 -0.08852152753078002 2109 18-19 -0.12445583981717334 2109 20-21 0.03931665258100736 2109 22-23 -0.018465365818535417 2109 24-25 -0.12300506045771442 2109 26-27 -0.13082971537004795 2109 28-29 -0.29709318685457475 2109 30-31 -0.09607096712898056 2109 32-33 -0.10088612322148549 2109 34-35 -0.19362215850604514 2109 36-37 -0.18430127416913678 2109 38-39 -0.010620408517823421 2109 40-41 -0.3886182921045531 2109 42-43 -0.19721504796429912 2109 44-45 -0.3438481057886449 2109 46-47 -0.2231496876621648 2109 48-49 -0.37428160266266275 2109 50-51 -0.16121888590566869 2109 52-53 -0.5706693882145615 2109 54-55 -0.3418829571005233 2109 56-57 -0.03350321948011015 2109 58-59 -0.03150385248162735 2109 60-61 -0.12350084853451193 2109 62-63 -0.023149701321941052 2109 64-65 -0.002793104656156231 2109 66-67 -0.14061082745548958 2109 68-69 0.01557074148594495 2109 70-71 -0.1955724247120898 2109 72-73 -0.37492031637494705 2109 74-75 -0.23541594446414393 2109 76 -0.42073241882179246 2108 1 0.014737538643238679 2108 2 0.011158453620296882 2108 3 -0.026001040279552967 2108 4 0.04733727284501299 2108 5 -0.01805293885231407 2108 6 0.06689484747716534 2108 7 0.047878629564245045 2108 8 0.08215007522882445 2108 9 -0.015272205654952131 2108 10-11 0.1498932919586906 2108 12-13 0.17277912397005224 2108 14-15 0.10645403990162805 2108 16-17 -0.004659046992685489 2108 18-19 -0.006228072821471642 2108 20-21 0.12912155373154377 2108 22-23 -0.05124296303520737 2108 24-25 0.0026944074545127705 2108 26-27 -0.033309727256131794 2108 28-29 -0.021955831445318097 2108 30-31 -0.14062178785656698 2108 32-33 -0.1569659766265019 2108 34-35 -0.06125664299378286 2108 36-37 -0.05795983415824679 2108 38-39 -0.008568976461930333 2108 40-41 -0.11349938488097422 2108 42-43 -0.028440923711436028 2108 44-45 -0.06295859989667463 2108 46-47 0.07732256731900122 2108 48-49 0.013091604752517583 2108 50-51 0.013974556940553384 2108 52-53 -0.1342336643554276 2108 54-55 -0.12244107662371562 2108 56-57 -0.19272740517534004 2108 58-59 -0.030875592708888178 2108 60-61 -0.13196364417751738 2108 62-63 -0.11324522330344422 2108 64-65 -0.12547425129137224 2108 66-67 -0.1757412802717795 2108 68-69 -0.19591056115406502 2108 70-71 -0.09145776738094469 2108 72-73 -0.01673569832357913 2108 74-75 -0.019619623577149525 2108 76 -0.1486348043950585 >>END_MODULE >>Per sequence quality scores pass #Quality Count 14 1.0 15 11.0 16 9.0 17 15.0 18 20.0 19 40.0 20 64.0 21 162.0 22 327.0 23 519.0 24 742.0 25 1047.0 26 1530.0 27 2232.0 28 3571.0 29 5853.0 30 9562.0 31 17315.0 32 32035.0 33 48672.0 34 86282.0 35 131566.0 36 253911.0 37 1503969.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.36858375149741 4.042239533593242 41.66500353663213 8.92417317827722 2 6.44953094969885 6.523740685082558 5.479803091754764 81.54692527346383 3 6.843585597214514 40.67693758618308 7.588636098416017 44.89084071818639 4 7.0353972816754835 3.674239266857351 79.45047643316956 9.839887018297606 5 6.923892152963507 4.13078632311719 43.89543953073536 45.049881993183945 6 44.43919969706423 4.062101831189523 6.071432824232955 45.42726564751328 7 7.249833885460751 4.745231500556097 43.356775925180585 44.64815868880257 8 7.900288408182124 4.97995908461958 80.14908631049487 6.97066619670343 9 9.35838110366738 4.293876267888571 79.30353353608437 7.044209092359684 10-11 64.21078327470701 23.350226606428812 4.818107556484897 7.620882562379284 12-13 26.19703685003965 23.719108054233125 23.371613109116414 26.712241986610813 14-15 7.329783205641465 42.01990516586447 23.922065488424376 26.728246140069682 16-17 25.96835845493235 5.10220509608446 42.37756941682485 26.551867032158345 18-19 26.245573255916415 42.042553900893324 5.161696726055095 26.55017611713516 20-21 26.276271699083807 4.336625457559224 42.397312635898366 26.989790207458604 22-23 26.067455601572785 4.082083207308564 42.37537837200607 27.47508281911258 24-25 8.121583934878338 23.788792805751967 22.79412990514205 45.295493354227645 26-27 42.66423905251601 6.7260789109554615 6.708169501132437 43.901512535396094 28-29 9.203626655489163 22.454089275550082 23.282804346842394 45.05947972211836 30-31 46.25467085505524 21.43525343481999 23.281875534364872 9.028200175759899 32-33 44.65466037614524 23.155819010171687 5.729534569685942 26.45998604399713 34-35 7.165454844233385 41.87624883600744 24.812344155983336 26.14595216377584 36-37 25.926323736398256 5.032663238792924 41.99839958465411 27.042613440154707 38-39 45.37370412797607 4.557420854459848 42.15539270906021 7.913482308503873 40-41 26.22940239252568 24.086203324196042 24.806914175345508 24.877480107932772 42-43 26.73993965100466 4.428339735788573 43.543991178663035 25.287729434543728 44-45 7.535169841697012 4.903224884553372 62.96793691696179 24.59366835678783 46-47 24.660423776646798 44.85268795949425 23.049720046392995 7.4371682174659615 48-49 7.951849408536978 23.50864867310802 42.1358400156231 26.4036619027319 50-51 27.738484511456544 6.170172735305115 40.66814959120343 25.423193162034906 52-53 44.99448666439624 23.68755224570186 23.06541459569269 8.252546494209213 54-55 43.210237895072765 25.89736383966315 4.850473098970923 26.041925166293154 56-57 25.862902515176557 23.138504992962456 22.32993800772105 28.668654484139932 58-59 7.633576332905445 23.185541009452454 42.96403114141527 26.21685151622683 60-61 25.060527613118644 24.33079060994401 24.387900669459455 26.22078110747789 62-63 7.915220854936162 6.109085453129503 42.63677954516768 43.33891414676666 64-65 44.49419015887457 24.21106906316163 23.992298001147915 7.302442776815887 66-67 26.2761764362656 23.816633364373136 23.114427315660492 26.79276288370077 68-69 8.471460450450236 23.517603378019533 41.497150450950365 26.513785720579865 70-71 45.41940646501116 5.943304333743757 23.342200713994824 25.29508848725026 72-73 60.79532545351055 26.57615905080128 4.585618648649293 8.042896847038875 74-75 8.101292954600122 41.55116446887406 23.265347435405857 27.082195141119957 76 8.293199901879298 77.96366199799472 4.507026823628037 9.236111276497947 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1.0 1 0.5 2 0.0 3 1.0 4 2.0 5 1.0 6 0.5 7 1.0 8 1.0 9 2.0 10 2.5 11 6.0 12 10.0 13 8.0 14 9.5 15 19.0 16 27.0 17 29.0 18 35.0 19 49.0 20 63.0 21 69.0 22 86.5 23 113.5 24 132.0 25 141.0 26 176.0 27 240.5 28 303.0 29 336.0 30 455.0 31 794.0 32 1814.0 33 2614.0 34 9415.5 35 11206.0 36 12009.5 37 17824.0 38 13288.0 39 11708.5 40 26399.0 41 353128.0 42 668123.0 43 359801.5 44 37411.5 45 17589.5 46 11836.0 47 11794.0 48 18759.0 49 20014.5 50 14263.0 51 21342.5 52 43023.0 53 357158.0 54 656692.0 55 367795.0 56 50258.0 57 18784.0 58 15950.0 59 15150.0 60 19490.5 61 21518.5 62 18406.0 63 19712.0 64 22674.0 65 25717.0 66 27921.0 67 28738.0 68 28980.5 69 28696.5 70 26755.5 71 25341.0 72 23787.5 73 20239.0 74 16224.5 75 14205.0 76 12404.5 77 8921.5 78 6005.5 79 4772.0 80 3879.5 81 2405.5 82 1474.5 83 1125.0 84 907.0 85 543.0 86 320.5 87 244.0 88 198.5 89 130.5 90 80.0 91 42.0 92 32.0 93 25.5 94 12.0 95 5.5 96 6.0 97 5.0 98 2.0 99 0.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 2099455.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 19.24126286371251 #Duplication Level Percentage of deduplicated Percentage of total 1 95.50119684412898 18.375636322770376 2 2.3856877913322148 0.9180729180754573 3 0.8193862288277364 0.4729807744734169 4 0.41563873729405443 0.31989656802465793 5 0.2651792802730357 0.25511921188717873 6 0.13061890638599233 0.1507963627646121 7 0.06197744245000046 0.08347669832607513 8 0.03515057913809214 0.0541073226406208 9 0.03675300273087948 0.06364567679180365 >10 0.21944726011402663 0.9104117517422384 >50 0.038988058884932696 0.5304527032239161 >100 0.05899103532363932 2.355006586351523 >500 0.014263098702905888 2.038694404972176 >1k 0.013033116534136321 5.19522505710427 >5k 0.0014754471517394003 1.917708461273433 >10k+ 0.0022131707276091006 66.35876917957825 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source TCATTGTTTGGCATAGTCACGTTCAGCACTGGATATGTGCTTCCTGATTT 664495 31.65083319242375 No Hit GCCTCCCTTAGGTCACTAGTTGCCTCGTCAGGCACTCTGGAGTTTATCAC 646734 30.80485173533131 No Hit GCCTCCCTTAGGTCACTAGTTGCCTCATCCGGCACACTGGAGTTTAACAA 17264 0.8223086467678516 No Hit GCCTCCCTTAGGTCACTAGTTGCCTCATCCGGCACCCTGGAGTTTAACAA 16356 0.7790593273016092 No Hit TCATTGTTTGGCATAGTCACGTTCAGTGCTGGATATTTGTATTTTAATTG 14531 0.6921320056871902 No Hit TCATTGTTTGGCATAGTCACGTTCAATGCTGGGTATGTGTAGTTTAAGTG 12308 0.5862473832494624 No Hit GCCTCCCTTAGGTCACTAGTTGCCTCGTCAGGCACTCTGGAGTTTAACAC 10547 0.5023684718176861 No Hit GATCTTTGGCGGCACGGAGCCGCGCATCACCTGTAATGCGTACCTGACCA 10197 0.48569747863135904 No Hit CTCCCTTAGGTCACTAGTTGCCTCGTCAGGCACTCTGGAGTTTATCACTG 10034 0.4779335589474411 No Hit CATTGTTTGGCATAGTCACGTTCAGCACTGGATATGTGCTTCCTGATTTG 7970 0.37962233055721606 No Hit GATCTCCCGAGCATCACCACATTACTGCGGTTATAGGTCCACGTCTTATC 7864 0.37457340119221416 No Hit GCCTCCCTTAGGTCACTAGTTGCCTCGTCCGGCACTCTGGAGTTTATCAC 6564 0.3126525693572856 No Hit TGGCATAGTCACGTTCAGCACTGGATATGTGCTTCCTGATTTGGTCAACC 6494 0.30931837072002016 No Hit CCTCCCTTAGGTCACTAGTTGCCTCGTCAGGCACTCTGGAGTTTATCACT 6000 0.2857884546227473 No Hit GCCTCCCTTAGGTCACTAGTTGCCTCGTCAGGCACCCTGGAGTTTATCAC 5638 0.2685458845271749 No Hit GATCTCGTCGAACTATAACCCGCAGACGCGGCAATACAGCGGTATCTGGG 4760 0.22672550733404623 No Hit TCATTGTTTGGCATAGTCACGTTCAGCACTGGATATGTGCTTCTTGATTT 4400 0.2095782000566814 No Hit ATTGTTTGGCATAGTCACGTTCAGCACTGGATATGTGCTTCCTGATTTGG 4241 0.20200480600917858 No Hit TGTTTGGCATAGTCACGTTCAGCACTGGATATGTGCTTCCTGATTTGGTC 4028 0.19185931587007105 No Hit GCCTCCCTTAGGTCACTAGTTGCCTCGTCAGGCACACTGGAGTTTATCAC 3655 0.17409280027435692 No Hit TTGTTTGGCATAGTCACGTTCAGCACTGGATATGTGCTTCCTGATTTGGT 3602 0.17156833559185597 No Hit TCATTGTTTGGCATAGTCACGTTCAGCGCTGGATATTTGTATTCTGATTC 3368 0.16042258586156885 No Hit GCCTCCCTTAGGTCACTAGTTGCCTCATCAGGCACCCTGGAGTTTATCAA 3132 0.1491815733130741 No Hit GGTCACTAGTTGCCTCGTCAGGCACTCTGGAGTTTATCACTGAGGGTTTC 2936 0.13984581712873104 No Hit GATCTCCCGAGGTTGTTCGGGTCAATCCAGTTCACCTCAACGGCATTATG 2688 0.12803322767099082 No Hit GCCTCCCTTAGGTCACTAGTTGCCTCGTCAGGCACACTGGAGTTTAACAC 2626 0.12508008030655574 No Hit GATCTGAATTCGTTGTTCGGGTCAATCCAGTTCACCTCAACGGCATTATG 2542 0.12107904194183729 No Hit TCATTGTTTGGCATAGTCACGTTCAGCACTGGATATTTGCTTCCTGATTT 2538 0.12088851630542212 No Hit GATCTGCTAGCGGCGACATACGGAAATAGCCTCCGGCTCACCGGAGATAT 2534 0.12069799066900695 No Hit TTGGCATAGTCACGTTCAGCACTGGATATGTGCTTCCTGATTTGGTCAAC 2526 0.12031693939617662 No Hit GCCTCCCTTAGGTCACTAGTTGCCTCGTCCGGCACTCTGGAGTTTAACAC 2489 0.11855457725933635 No Hit GATCTCCATGGCATCACCACATTACTGCGGTTATAGGTCCACGTCTTATC 2481 0.11817352598650602 No Hit TCCCTTAGGTCACTAGTTGCCTCGTCAGGCACTCTGGAGTTTATCACTGA 2365 0.11264828253046623 No Hit GATCTCATGGTGATGATTCTGCTGCTTGATAAATTTTCAGGTATTCGTCA 2357 0.11226723125763591 No Hit TCATTGTTTGGCATAGTCACGTCCAGCACTGGATATGTGCTTCCTGATTT 2353 0.11207670562122074 No Hit CCCTTAGGTCACTAGTTGCCTCGTCAGGCACTCTGGAGTTTATCACTGAG 2101 0.10007359052706535 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 8.57365363868242E-4 4.763140910379122E-5 0.0 2 8.57365363868242E-4 4.763140910379122E-5 0.0 3 9.049967729720332E-4 4.763140910379122E-5 0.0 4 9.049967729720332E-4 4.763140910379122E-5 0.0 5 9.049967729720332E-4 4.763140910379122E-5 0.0 6 9.049967729720332E-4 4.763140910379122E-5 0.0 7 9.049967729720332E-4 4.763140910379122E-5 0.0 8 9.049967729720332E-4 4.763140910379122E-5 0.0 9 9.049967729720332E-4 4.763140910379122E-5 0.0 10 9.049967729720332E-4 4.763140910379122E-5 0.0 11 9.526281820758244E-4 4.763140910379122E-5 0.0 12 9.526281820758244E-4 4.763140910379122E-5 0.0 13 9.526281820758244E-4 4.763140910379122E-5 0.0 14 9.526281820758244E-4 4.763140910379122E-5 0.0 15 0.0010002595911796158 4.763140910379122E-5 0.0 16 0.0010002595911796158 4.763140910379122E-5 0.0 17 0.0010002595911796158 4.763140910379122E-5 0.0 18 0.0010002595911796158 4.763140910379122E-5 0.0 19 0.0010002595911796158 4.763140910379122E-5 0.0 20 0.001047891000283407 4.763140910379122E-5 0.0 21 0.001047891000283407 4.763140910379122E-5 0.0 22 0.001047891000283407 4.763140910379122E-5 0.0 23 0.001047891000283407 4.763140910379122E-5 0.0 24 0.001095522409387198 4.763140910379122E-5 0.0 25 0.0011431538184909892 4.763140910379122E-5 0.0 26 0.0011907852275947805 4.763140910379122E-5 0.0 27 0.001286048045802363 4.763140910379122E-5 0.0 28 0.0014289422731137366 4.763140910379122E-5 0.0 29 0.001524205091321319 4.763140910379122E-5 0.0 30 0.001524205091321319 4.763140910379122E-5 0.0 31 0.0015718365004251103 4.763140910379122E-5 0.0 32 0.0018099935459440664 4.763140910379122E-5 0.0 33 0.0018576249550478576 4.763140910379122E-5 0.0 34 0.0020005191823592315 4.763140910379122E-5 0.0 35 0.002095782000566814 4.763140910379122E-5 0.0 36 0.002381570455189561 4.763140910379122E-5 0.0 37 0.002524464682500935 9.526281820758244E-5 0.0 38 0.0027626217280198907 9.526281820758244E-5 0.0 39 0.0029055159553312646 9.526281820758244E-5 0.0 40 0.003429461455472968 9.526281820758244E-5 0.0 41 0.0038105127283032977 9.526281820758244E-5 0.0 42 0.004143932592029836 1.4289422731137365E-4 0.0 43 0.004429721046652584 1.4289422731137365E-4 0.0 44 0.004810772319482914 1.4289422731137365E-4 0.0 45 0.005096560774105661 1.4289422731137365E-4 0.0 46 0.005572874865143573 1.4289422731137365E-4 0.0 47 0.006430240229011815 1.4289422731137365E-4 0.0 48 0.006906554320049727 1.4289422731137365E-4 0.0 49 0.0076210254566065954 1.4289422731137365E-4 0.0 50 0.008383128002267256 1.4289422731137365E-4 0.0 51 0.00895470491151275 1.4289422731137365E-4 0.0 52 0.00971680745717341 1.4289422731137365E-4 0.0 53 0.010812329866560607 1.4289422731137365E-4 0.0 54 0.011955483685051596 1.4289422731137365E-4 0.0 55 0.012812849048919838 1.4289422731137365E-4 0.0 56 0.013813108640099454 1.4289422731137365E-4 0.0 57 0.014908631049486653 1.4289422731137365E-4 0.0 58 0.015765996413354893 1.4289422731137365E-4 0.0 59 0.016766256004534512 1.4289422731137365E-4 0.0 60 0.018052304050336872 1.4289422731137365E-4 0.0 61 0.01957650914165819 1.4289422731137365E-4 0.0 62 0.021291239869394678 1.4289422731137365E-4 0.0 63 0.02252965650609325 1.4289422731137365E-4 0.0 64 0.024720701324867644 1.4289422731137365E-4 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGGTAAT 15 0.0022251606 70.00001 33 ATTAGTA 15 0.0022251606 70.00001 15 ATCTAAG 30 1.0247095E-7 70.00001 2 TTTAACG 30 1.0247095E-7 70.00001 43 GGTCCTA 15 0.0022251606 70.00001 11 TATATGT 30 1.0247095E-7 70.00001 32 TCTAAGC 30 1.0247095E-7 70.00001 3 TAGCTTA 15 0.0022251606 70.00001 40 CTTAATT 210 0.0 70.0 43 CTAGATA 55 0.0 70.0 29 TCTTAAT 200 0.0 70.0 42 CCTAAGT 105 0.0 70.0 43 TCTAGAG 40 1.3460522E-10 70.0 36 GGGGTTA 35 3.7161954E-9 70.0 70 AGGTTAC 25 2.8418126E-6 70.0 10 GTACTAG 35 3.7161954E-9 70.0 12 GTATACT 50 0.0 70.0 65 TACTAAG 25 2.8418126E-6 70.0 68 ATAGTAC 40 1.3460522E-10 70.0 13 TTATTGT 20 7.930142E-5 70.0 1 >>END_MODULE