FastQCFastQC Report
Tue 25 Aug 2015
000000000-AHV5L l01n01 kokuya6.34100000033d21.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AHV5L l01n01 kokuya6.34100000033d21.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2099455
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCATTGTTTGGCATAGTCACGTTCAGCACTGGATATGTGCTTCCTGATTT66449531.65083319242375No Hit
GCCTCCCTTAGGTCACTAGTTGCCTCGTCAGGCACTCTGGAGTTTATCAC64673430.80485173533131No Hit
GCCTCCCTTAGGTCACTAGTTGCCTCATCCGGCACACTGGAGTTTAACAA172640.8223086467678516No Hit
GCCTCCCTTAGGTCACTAGTTGCCTCATCCGGCACCCTGGAGTTTAACAA163560.7790593273016092No Hit
TCATTGTTTGGCATAGTCACGTTCAGTGCTGGATATTTGTATTTTAATTG145310.6921320056871902No Hit
TCATTGTTTGGCATAGTCACGTTCAATGCTGGGTATGTGTAGTTTAAGTG123080.5862473832494624No Hit
GCCTCCCTTAGGTCACTAGTTGCCTCGTCAGGCACTCTGGAGTTTAACAC105470.5023684718176861No Hit
GATCTTTGGCGGCACGGAGCCGCGCATCACCTGTAATGCGTACCTGACCA101970.48569747863135904No Hit
CTCCCTTAGGTCACTAGTTGCCTCGTCAGGCACTCTGGAGTTTATCACTG100340.4779335589474411No Hit
CATTGTTTGGCATAGTCACGTTCAGCACTGGATATGTGCTTCCTGATTTG79700.37962233055721606No Hit
GATCTCCCGAGCATCACCACATTACTGCGGTTATAGGTCCACGTCTTATC78640.37457340119221416No Hit
GCCTCCCTTAGGTCACTAGTTGCCTCGTCCGGCACTCTGGAGTTTATCAC65640.3126525693572856No Hit
TGGCATAGTCACGTTCAGCACTGGATATGTGCTTCCTGATTTGGTCAACC64940.30931837072002016No Hit
CCTCCCTTAGGTCACTAGTTGCCTCGTCAGGCACTCTGGAGTTTATCACT60000.2857884546227473No Hit
GCCTCCCTTAGGTCACTAGTTGCCTCGTCAGGCACCCTGGAGTTTATCAC56380.2685458845271749No Hit
GATCTCGTCGAACTATAACCCGCAGACGCGGCAATACAGCGGTATCTGGG47600.22672550733404623No Hit
TCATTGTTTGGCATAGTCACGTTCAGCACTGGATATGTGCTTCTTGATTT44000.2095782000566814No Hit
ATTGTTTGGCATAGTCACGTTCAGCACTGGATATGTGCTTCCTGATTTGG42410.20200480600917858No Hit
TGTTTGGCATAGTCACGTTCAGCACTGGATATGTGCTTCCTGATTTGGTC40280.19185931587007105No Hit
GCCTCCCTTAGGTCACTAGTTGCCTCGTCAGGCACACTGGAGTTTATCAC36550.17409280027435692No Hit
TTGTTTGGCATAGTCACGTTCAGCACTGGATATGTGCTTCCTGATTTGGT36020.17156833559185597No Hit
TCATTGTTTGGCATAGTCACGTTCAGCGCTGGATATTTGTATTCTGATTC33680.16042258586156885No Hit
GCCTCCCTTAGGTCACTAGTTGCCTCATCAGGCACCCTGGAGTTTATCAA31320.1491815733130741No Hit
GGTCACTAGTTGCCTCGTCAGGCACTCTGGAGTTTATCACTGAGGGTTTC29360.13984581712873104No Hit
GATCTCCCGAGGTTGTTCGGGTCAATCCAGTTCACCTCAACGGCATTATG26880.12803322767099082No Hit
GCCTCCCTTAGGTCACTAGTTGCCTCGTCAGGCACACTGGAGTTTAACAC26260.12508008030655574No Hit
GATCTGAATTCGTTGTTCGGGTCAATCCAGTTCACCTCAACGGCATTATG25420.12107904194183729No Hit
TCATTGTTTGGCATAGTCACGTTCAGCACTGGATATTTGCTTCCTGATTT25380.12088851630542212No Hit
GATCTGCTAGCGGCGACATACGGAAATAGCCTCCGGCTCACCGGAGATAT25340.12069799066900695No Hit
TTGGCATAGTCACGTTCAGCACTGGATATGTGCTTCCTGATTTGGTCAAC25260.12031693939617662No Hit
GCCTCCCTTAGGTCACTAGTTGCCTCGTCCGGCACTCTGGAGTTTAACAC24890.11855457725933635No Hit
GATCTCCATGGCATCACCACATTACTGCGGTTATAGGTCCACGTCTTATC24810.11817352598650602No Hit
TCCCTTAGGTCACTAGTTGCCTCGTCAGGCACTCTGGAGTTTATCACTGA23650.11264828253046623No Hit
GATCTCATGGTGATGATTCTGCTGCTTGATAAATTTTCAGGTATTCGTCA23570.11226723125763591No Hit
TCATTGTTTGGCATAGTCACGTCCAGCACTGGATATGTGCTTCCTGATTT23530.11207670562122074No Hit
CCCTTAGGTCACTAGTTGCCTCGTCAGGCACTCTGGAGTTTATCACTGAG21010.10007359052706535No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGTAAT150.002225160670.0000133
ATTAGTA150.002225160670.0000115
ATCTAAG301.0247095E-770.000012
TTTAACG301.0247095E-770.0000143
GGTCCTA150.002225160670.0000111
TATATGT301.0247095E-770.0000132
TCTAAGC301.0247095E-770.000013
TAGCTTA150.002225160670.0000140
CTTAATT2100.070.043
CTAGATA550.070.029
TCTTAAT2000.070.042
CCTAAGT1050.070.043
TCTAGAG401.3460522E-1070.036
GGGGTTA353.7161954E-970.070
AGGTTAC252.8418126E-670.010
GTACTAG353.7161954E-970.012
GTATACT500.070.065
TACTAAG252.8418126E-670.068
ATAGTAC401.3460522E-1070.013
TTATTGT207.930142E-570.01
Produced by FastQC (version 0.11.2)