##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename 000000000-AHV5L l01n01 kokuya5.34100000033d14.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2353186 Sequences flagged as poor quality 0 Sequence length 76 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 33.585204484473394 34.0 34.0 34.0 34.0 34.0 2 33.7881200211118 34.0 34.0 34.0 34.0 34.0 3 33.79311410147774 34.0 34.0 34.0 34.0 34.0 4 33.83933441725389 34.0 34.0 34.0 34.0 34.0 5 33.892110100943995 34.0 34.0 34.0 34.0 34.0 6 37.71233595644373 38.0 38.0 38.0 37.0 38.0 7 37.72064129227354 38.0 38.0 38.0 38.0 38.0 8 37.71867714664289 38.0 38.0 38.0 38.0 38.0 9 37.70130877882156 38.0 38.0 38.0 38.0 38.0 10-11 37.50337457387559 38.0 38.0 38.0 37.0 38.0 12-13 37.500407957552014 38.0 38.0 38.0 37.0 38.0 14-15 37.48921313487332 38.0 38.0 38.0 37.0 38.0 16-17 37.68241311991487 38.0 38.0 38.0 37.0 38.0 18-19 37.64814489802336 38.0 38.0 38.0 37.0 38.0 20-21 37.56089637623205 38.0 38.0 38.0 37.0 38.0 22-23 37.55543399459286 38.0 38.0 38.0 37.0 38.0 24-25 37.65893452536264 38.0 38.0 38.0 37.5 38.0 26-27 37.16454989108384 38.0 38.0 38.0 36.5 38.0 28-29 37.45747786192847 38.0 38.0 38.0 37.0 38.0 30-31 37.35604006653108 38.0 38.0 38.0 37.0 38.0 32-33 37.36589032911125 38.0 38.0 38.0 37.0 38.0 34-35 37.48216184356018 38.0 38.0 38.0 37.0 38.0 36-37 37.092199469145235 38.0 38.0 38.0 36.5 38.0 38-39 37.564594978892444 38.0 38.0 38.0 37.0 38.0 40-41 37.13335112481546 38.0 38.0 38.0 36.5 38.0 42-43 37.17196005755601 38.0 38.0 38.0 37.0 38.0 44-45 37.266119635251954 38.0 38.0 38.0 37.0 38.0 46-47 37.07902520242769 38.0 38.0 38.0 36.5 38.0 48-49 37.32306902216824 38.0 38.0 38.0 37.0 38.0 50-51 37.06013889254823 38.0 38.0 38.0 36.5 38.0 52-53 37.07421640278329 38.0 38.0 38.0 36.5 38.0 54-55 36.995555387461934 38.0 38.0 38.0 37.0 38.0 56-57 37.15192317989313 38.0 38.0 38.0 37.0 38.0 58-59 37.28981007026219 38.0 38.0 38.0 36.5 38.0 60-61 37.24095630349662 38.0 38.0 38.0 37.0 38.0 62-63 37.045108843924794 38.0 38.0 38.0 36.5 38.0 64-65 37.230679385309955 38.0 38.0 38.0 37.0 38.0 66-67 37.342680519091985 38.0 38.0 38.0 37.0 38.0 68-69 37.444196081397735 38.0 38.0 38.0 37.0 38.0 70-71 37.385565781880395 38.0 38.0 38.0 37.0 38.0 72-73 36.981581353960124 38.0 38.0 38.0 36.5 38.0 74-75 37.09408563538963 38.0 38.0 38.0 36.5 38.0 76 36.056401831389444 38.0 37.0 38.0 33.0 38.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.02502926195725763 1101 2 -0.016470330888182616 1101 3 0.03585452351344287 1101 4 1.087168933366911E-5 1101 5 0.010632148355185222 1101 6 -0.07156853584238831 1101 7 -0.048444373145599684 1101 8 -0.11694287102764633 1101 9 -0.40633578146002236 1101 10-11 -0.5623697153587415 1101 12-13 -0.8190839600220485 1101 14-15 -0.36033571161379285 1101 16-17 -0.06339317068149342 1101 18-19 0.041960632017108423 1101 20-21 0.1449445681523045 1101 22-23 0.09705603084822201 1101 24-25 0.1050600864428759 1101 26-27 0.13873809942216297 1101 28-29 0.1176149705131806 1101 30-31 0.12407196396934239 1101 32-33 0.169544634355681 1101 34-35 0.11416952257221169 1101 36-37 0.09322086127569662 1101 38-39 0.07789307270695645 1101 40-41 0.17114765844622326 1101 42-43 0.15722693496931583 1101 44-45 0.18849223056405862 1101 46-47 0.2072984325250644 1101 48-49 0.1411714247102296 1101 50-51 0.2242874280742413 1101 52-53 0.32137455499083245 1101 54-55 0.28344924254588477 1101 56-57 0.15462775151129193 1101 58-59 0.14903213761938616 1101 60-61 0.1664062163782063 1101 62-63 0.1174536097061818 1101 64-65 0.17317805871485348 1101 66-67 0.17577244349048726 1101 68-69 0.07845358506378375 1101 70-71 0.10829312167753358 1101 72-73 0.14907406440681115 1101 74-75 0.1788165241939481 1101 76 0.11959987752017298 1102 1 0.02453211292136359 1102 2 0.005347655876960289 1102 3 0.019030593648174943 1102 4 0.002716096639481691 1102 5 0.02120798158937731 1102 6 -0.04797581079967017 1102 7 0.011783301729096252 1102 8 -0.04879283539135315 1102 9 -0.24799893339005763 1102 10-11 -0.22553682802596597 1102 12-13 -0.011082034829883014 1102 14-15 0.15324428146167435 1102 16-17 0.03881132474457871 1102 18-19 0.0668712153158566 1102 20-21 0.12623031471080282 1102 22-23 0.10994668664103813 1102 24-25 0.04448808603720522 1102 26-27 0.10670746172670675 1102 28-29 0.12357852859150853 1102 30-31 0.154955526584736 1102 32-33 0.1499215387860957 1102 34-35 0.09755224521057926 1102 36-37 0.1546912675363643 1102 38-39 0.0789509676889395 1102 40-41 0.1778490896252478 1102 42-43 0.20005000860271593 1102 44-45 0.1666687111445455 1102 46-47 0.2560978165047416 1102 48-49 0.07933210334362428 1102 50-51 0.175575222715608 1102 52-53 0.22700055253055496 1102 54-55 0.27112175950132666 1102 56-57 0.2094966431856662 1102 58-59 0.13738438476771364 1102 60-61 0.16155626582469296 1102 62-63 0.19182432173821695 1102 64-65 0.1231303535097723 1102 66-67 0.19877659132289693 1102 68-69 0.08527351797941662 1102 70-71 0.10733255566440647 1102 72-73 0.23648115405590886 1102 74-75 0.22609402792662792 1102 76 0.5930400914426102 1103 1 0.06455770988543463 1103 2 0.06915463365439933 1103 3 0.006068447500744867 1103 4 -0.00828245347279477 1103 5 0.02371084499342402 1103 6 0.0701845390802589 1103 7 0.049561080208867736 1103 8 0.06682743250374301 1103 9 0.07699173252875369 1103 10-11 0.19891740786637513 1103 12-13 0.19547508648750522 1103 14-15 0.17406952767507988 1103 16-17 0.06974970079738796 1103 18-19 0.06171336904598945 1103 20-21 0.1384238655750707 1103 22-23 0.14019389124848658 1103 24-25 0.04821950722383406 1103 26-27 0.08362362776373544 1103 28-29 -0.01727190219489927 1103 30-31 0.08430610226800894 1103 32-33 0.06751267986786758 1103 34-35 0.10370663621192477 1103 36-37 0.005897828735641042 1103 38-39 0.11061226298113525 1103 40-41 0.07102064887443049 1103 42-43 0.03932017463348103 1103 44-45 -0.023822504337054795 1103 46-47 0.0753363019416966 1103 48-49 -0.12540774035614533 1103 50-51 0.10362016139497854 1103 52-53 0.046729843642658864 1103 54-55 0.22318705617003332 1103 56-57 0.20072150122306454 1103 58-59 0.1416917319022275 1103 60-61 0.07305503251407686 1103 62-63 0.1152029664919425 1103 64-65 0.1264425918148291 1103 66-67 0.17137620609335613 1103 68-69 0.05643639789001753 1103 70-71 0.14632446111129127 1103 72-73 0.24555876878254423 1103 74-75 0.17437995863970457 1103 76 0.4117810368238537 2116 1 -0.0032697656001872133 2116 2 0.05186480135668603 2116 3 1.8425942163702302E-4 2116 4 -0.0010963300791928532 2116 5 0.018002398911818318 2116 6 0.043641273975509876 2116 7 0.06003368743846238 2116 8 0.054823620306962084 2116 9 0.07801172697786285 2116 10-11 0.18959515990583498 2116 12-13 0.15755987817584582 2116 14-15 0.1442159268111851 2116 16-17 0.03191966569066551 2116 18-19 0.049523137868760614 2116 20-21 0.16417680555654357 2116 22-23 0.13584400560354482 2116 24-25 0.05199931620404641 2116 26-27 0.09990582693217931 2116 28-29 0.0826606044278293 2116 30-31 0.1797503935107585 2116 32-33 0.024392099789096733 2116 34-35 0.12243863200668414 2116 36-37 0.11155739478994064 2116 38-39 0.13658595101259152 2116 40-41 0.14869227409565156 2116 42-43 0.06644716238652393 2116 44-45 0.10041305651372312 2116 46-47 0.16197306715615412 2116 48-49 0.07391124798430582 2116 50-51 0.2086990737198775 2116 52-53 0.17491490327761028 2116 54-55 0.3036577923582868 2116 56-57 0.20066885855843708 2116 58-59 0.18851665410441854 2116 60-61 0.13744841630176552 2116 62-63 0.1315071619581616 2116 64-65 0.1674095045298145 2116 66-67 0.2218699566040101 2116 68-69 0.004865014351153718 2116 70-71 0.1434937331426056 2116 72-73 0.32250712832691875 2116 74-75 0.19381859085309827 2116 76 0.5404956348460104 1117 1 0.02362500568971626 1117 2 -0.036369834920023436 1117 3 -0.008643954253948039 1117 4 0.0248991273344501 1117 5 0.03726536949535131 1117 6 0.04721821696335127 1117 7 0.057308301088397684 1117 8 0.04639292715771859 1117 9 0.06532892787419087 1117 10-11 0.17760302918040338 1117 12-13 0.18572539426584456 1117 14-15 0.13304665273433613 1117 16-17 0.09530895525409733 1117 18-19 0.024283380270119892 1117 20-21 0.16064399664752926 1117 22-23 0.07898437725071261 1117 24-25 -0.024721931413587583 1117 26-27 0.1276561774229492 1117 28-29 0.12246520714152354 1117 30-31 -0.004230915778187239 1117 32-33 0.15452616696727262 1117 34-35 0.17459458603626388 1117 36-37 0.16080132695320515 1117 38-39 0.068649239452796 1117 40-41 0.1912632305396258 1117 42-43 -0.14076373557565347 1117 44-45 0.037255492260705125 1117 46-47 -0.11196061270339897 1117 48-49 0.007832047653870688 1117 50-51 -0.19427734624346726 1117 52-53 0.03213850512168648 1117 54-55 -0.09929416414507841 1117 56-57 0.10734927459302668 1117 58-59 0.08664813931060422 1117 60-61 0.18369204417668072 1117 62-63 0.1490932229839146 1117 64-65 0.2069290045178036 1117 66-67 0.17288567946230415 1117 68-69 0.18230057217709117 1117 70-71 0.0990635965335116 1117 72-73 0.12670924038365428 1117 74-75 0.28411618862976695 1117 76 0.6063356638449804 2117 1 -0.05696030019542775 2117 2 -0.01842017079161451 2117 3 -0.03229576331425932 2117 4 0.020865618594022806 2117 5 -0.005889912231566541 2117 6 -0.016668958516270038 2117 7 0.01911292897946737 2117 8 -0.04701092432981113 2117 9 -0.19615310192340019 2117 10-11 -0.3238878914855974 2117 12-13 -0.8589449109013003 2117 14-15 -1.2396773607691003 2117 16-17 -0.10888134599323251 2117 18-19 0.06755050509318039 2117 20-21 0.17535995676908556 2117 22-23 0.15241973095101002 2117 24-25 0.0622293924114814 2117 26-27 0.18364952460462547 2117 28-29 0.17719052773954047 2117 30-31 0.14281904893331898 2117 32-33 0.1232381350809959 2117 34-35 0.16137632194974572 2117 36-37 0.2188477948387515 2117 38-39 0.10343132491308182 2117 40-41 0.30250493134905554 2117 42-43 0.2547313597361054 2117 44-45 0.25348218711290116 2117 46-47 0.09407534716469712 2117 48-49 0.2088352662229127 2117 50-51 0.25946440859917885 2117 52-53 0.2924626823429364 2117 54-55 0.24520692936270905 2117 56-57 0.1152040348965997 2117 58-59 0.19226692032808046 2117 60-61 0.21979246934735386 2117 62-63 0.22729508417072708 2117 64-65 0.20123558694017873 2117 66-67 0.19811214652632003 2117 68-69 0.22527758673052745 2117 70-71 0.18460745413386803 2117 72-73 0.17330158620256952 2117 74-75 0.2610320395080521 2117 76 0.2591626703132377 1116 1 0.04902376971977418 1116 2 0.011217451978573934 1116 3 0.0447092016825863 1116 4 0.0037908829043189485 1116 5 -0.014990573969356547 1116 6 -0.014947466409850563 1116 7 0.02858741838271328 1116 8 -0.09473756520551291 1116 9 -0.1943322627588273 1116 10-11 -0.23984420705269116 1116 12-13 0.004974358030011672 1116 14-15 0.14407028513756615 1116 16-17 0.03137706630727166 1116 18-19 0.05708745355994438 1116 20-21 0.17798264056899882 1116 22-23 0.10082523030151691 1116 24-25 0.06865632245387587 1116 26-27 0.09089824722921236 1116 28-29 0.10802926822303505 1116 30-31 0.06696167989473167 1116 32-33 0.061264419249710045 1116 34-35 0.0849256192950989 1116 36-37 0.007935168416672411 1116 38-39 -0.019503002362043276 1116 40-41 0.1632244789233397 1116 42-43 0.16439739655027807 1116 44-45 0.16593622437315503 1116 46-47 0.026261163074984495 1116 48-49 0.12010381427941041 1116 50-51 0.12523612712265475 1116 52-53 0.11077477975174332 1116 54-55 0.09310422727979528 1116 56-57 0.14945246065803275 1116 58-59 0.08992335071366853 1116 60-61 0.11265884009330307 1116 62-63 -0.0109589914147179 1116 64-65 0.15169615676034454 1116 66-67 0.08058771814494747 1116 68-69 0.1537027434475675 1116 70-71 0.11151629201674496 1116 72-73 0.10610311826771834 1116 74-75 0.18616755203677826 1116 76 0.19602702584533915 2118 1 -0.12257761589552985 2118 2 0.03001752894356713 2118 3 -0.002470404625526612 2118 4 -0.0385736416128708 2118 5 -0.013933504785718753 2118 6 0.07089393761992113 2118 7 0.03621755775260738 2118 8 -0.012484439088531474 2118 9 0.12287789244702907 2118 10-11 0.19727309142034244 2118 12-13 0.2193121921639829 2118 14-15 0.17075324856642027 2118 16-17 0.09122868554835861 2118 18-19 0.07210530596779563 2118 20-21 0.16346576352894004 2118 22-23 0.09140406215080787 2118 24-25 0.06443723677632107 2118 26-27 0.013863256554436987 2118 28-29 0.06770461442742004 2118 30-31 0.13657561031259746 2118 32-33 0.11615956226004442 2118 34-35 0.13681956838853182 2118 36-37 0.18045863043763433 2118 38-39 0.05596418087829136 2118 40-41 0.11050521754178533 2118 42-43 0.12113429628151096 2118 44-45 0.14637455885209505 2118 46-47 0.1048071969930291 2118 48-49 0.09160512197574633 2118 50-51 0.20503540838478784 2118 52-53 0.2547616973769493 2118 54-55 0.266766418027224 2118 56-57 0.16600114706771052 2118 58-59 0.04476786899154206 2118 60-61 0.07218676676433944 2118 62-63 0.06281004990308503 2118 64-65 0.06975506633111905 2118 66-67 0.2040613820428021 2118 68-69 0.16298029958930726 2118 70-71 0.09335961693558659 2118 72-73 0.21945548978077767 2118 74-75 0.2404584562710923 2118 76 0.36514398088804256 1119 1 -0.0293410822706619 1119 2 0.01916540503106745 1119 3 0.02437676993624649 1119 4 0.01702808173460113 1119 5 0.015077074677080304 1119 6 0.10624168722699068 1119 7 0.10058931373863089 1119 8 0.08285379690773453 1119 9 0.12222632487684848 1119 10-11 0.2386638864949049 1119 12-13 0.2513744840293697 1119 14-15 0.1517473373247995 1119 16-17 0.032093134367045195 1119 18-19 0.05096348436092768 1119 20-21 0.14675660707968774 1119 22-23 0.08908740376593016 1119 24-25 0.06526318711645729 1119 26-27 0.09313741306693402 1119 28-29 0.07577756169289529 1119 30-31 0.12553517529388358 1119 32-33 0.18229814162880587 1119 34-35 0.20202485172960394 1119 36-37 0.06067652644432542 1119 38-39 0.0049058251050126955 1119 40-41 0.1599030509946502 1119 42-43 0.17939651292273595 1119 44-45 0.1835607652752742 1119 46-47 0.0452513025517689 1119 48-49 0.07532605862181896 1119 50-51 0.14896650078252804 1119 52-53 0.20800530325383448 1119 54-55 0.07979789200009435 1119 56-57 0.12641132765638474 1119 58-59 0.09316882356171163 1119 60-61 0.16606249959679076 1119 62-63 0.1388479574859005 1119 64-65 0.15785785199279445 1119 66-67 0.14066449816818505 1119 68-69 0.09267965477976503 1119 70-71 0.16448123966922168 1119 72-73 0.20504620807121654 1119 74-75 0.19138288273424564 1119 76 0.14456972226970066 2119 1 -0.032949303171200484 2119 2 0.006356601762632863 2119 3 -0.010655456613960723 2119 4 0.011466084132351284 2119 5 0.026005913039519157 2119 6 0.08378915478127169 2119 7 0.0729580308752702 2119 8 0.0907089840165014 2119 9 0.1310572409888877 2119 10-11 0.21010744426541805 2119 12-13 0.26886202766776535 2119 14-15 0.20418796823953755 2119 16-17 0.04619019362118593 2119 18-19 0.08561732194494454 2119 20-21 0.15860870016579298 2119 22-23 0.09327742392532912 2119 24-25 -0.021603856032697877 2119 26-27 0.09463399277075268 2119 28-29 0.08073777509235214 2119 30-31 0.0972398899077831 2119 32-33 0.09500495543471033 2119 34-35 0.14572668497832808 2119 36-37 0.08471811036486088 2119 38-39 0.005018475044536785 2119 40-41 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-0.014722311391402343 2104 48-49 -0.06935122480155798 2104 50-51 -0.09501999413222251 2104 52-53 -0.08717299654402666 2104 54-55 -0.06906234600734962 2104 56-57 -0.028180487864311488 2104 58-59 0.06955655834569541 2104 60-61 -0.07701850811989175 2104 62-63 -0.06249254664681558 2104 64-65 -0.23410407791778454 2104 66-67 -0.17566346142757538 2104 68-69 -0.03007824800413772 2104 70-71 -0.1598621323892715 2104 72-73 -0.18814118073809283 2104 74-75 -0.07558791182767521 2104 76 -0.40149923754155736 2111 1 0.06100241494773684 2111 2 -0.031300210515695426 2111 3 -0.009774464306666175 2111 4 0.017101263886289075 2111 5 -0.031101072577143896 2111 6 -0.18256840032233157 2111 7 -0.054641292807822595 2111 8 -0.11243987306139758 2111 9 -0.060593586158375956 2111 10-11 -0.31165787923902144 2111 12-13 -0.46083659969824 2111 14-15 -0.3720410789830595 2111 16-17 -0.08200970536236696 2111 18-19 -0.0647225152805504 2111 20-21 0.10531851022214056 2111 22-23 -0.05543558533049975 2111 24-25 -0.01476566037623428 2111 26-27 0.0020274938941824416 2111 28-29 0.05175210226666849 2111 30-31 -0.1076938276362327 2111 32-33 -0.005544438062472068 2111 34-35 -0.002358753917818035 2111 36-37 0.053637341147648954 2111 38-39 0.00692566820708862 2111 40-41 0.0031267399680956487 2111 42-43 0.08901883434859315 2111 44-45 0.09663943729319868 2111 46-47 0.06471345931201711 2111 48-49 0.09078705850688351 2111 50-51 0.008114903604329982 2111 52-53 -0.150577417774862 2111 54-55 -0.07279200182239975 2111 56-57 -0.1683134702597684 2111 58-59 -0.24350875599388644 2111 60-61 -0.05036792544535729 2111 62-63 -0.00880787727938781 2111 64-65 -0.028894093620451144 2111 66-67 -0.049707004188569215 2111 68-69 -0.15222551592428601 2111 70-71 -0.040112928368102985 2111 72-73 -0.012481582549675352 2111 74-75 0.03838587717816466 2111 76 -0.22152878478297566 2110 1 -0.016715405637356184 2110 2 0.012103309495365977 2110 3 -0.006238986721342599 2110 4 -0.03624389196458111 2110 5 0.014246161880869579 2110 6 -0.10499433346564047 2110 7 -0.0481000953912627 2110 8 -0.0665081035753019 2110 9 -0.09795461628748825 2110 10-11 -0.39321871372245454 2110 12-13 -0.44313665642347644 2110 14-15 -0.18445802513831921 2110 16-17 -0.08637475107575909 2110 18-19 -0.10144137707323608 2110 20-21 -0.05777111989798556 2110 22-23 -0.09421350141845153 2110 24-25 -0.07345079489325457 2110 26-27 -0.0267429877166947 2110 28-29 -0.09570396203892528 2110 30-31 -0.06622530750579614 2110 32-33 -0.12739355479357783 2110 34-35 -0.053757192824093636 2110 36-37 -0.025765063691849832 2110 38-39 -0.026450644781469634 2110 40-41 -0.1829537102717964 2110 42-43 -0.03302405332767222 2110 44-45 -0.1535655014785604 2110 46-47 0.051032761900650314 2110 48-49 -0.10547500768895901 2110 50-51 0.010935127666819255 2110 52-53 -0.16381942098855262 2110 54-55 -0.09750017735983363 2110 56-57 -0.10896998321174323 2110 58-59 -0.0663764078019966 2110 60-61 -0.1894864565825003 2110 62-63 -0.27403200494030955 2110 64-65 -0.2758038644654377 2110 66-67 -0.43000460003744934 2110 68-69 -0.3385316661086364 2110 70-71 -0.3341975759532403 2110 72-73 -0.39154917960980384 2110 74-75 -0.17633942577750616 2110 76 -0.2985320109300673 2109 1 0.03972102733199989 2109 2 -0.01018007621242134 2109 3 0.023300273359076584 2109 4 -0.010294136993593384 2109 5 0.0062137245781457295 2109 6 -0.22004345058042674 2109 7 -0.10593292208250915 2109 8 -0.11156520191691754 2109 9 -0.1755064535042834 2109 10-11 -0.5004167498178944 2109 12-13 -0.5224476847144501 2109 14-15 -0.1347005130117438 2109 16-17 -0.08262273629866002 2109 18-19 -0.05378227087907561 2109 20-21 0.03215940706768805 2109 22-23 -0.007835900655138062 2109 24-25 -0.08089396053749454 2109 26-27 -0.15815384997092252 2109 28-29 -0.21044869904276453 2109 30-31 -0.08647686815940148 2109 32-33 -0.09471756864501657 2109 34-35 -0.13703356843869585 2109 36-37 -0.2052827724976396 2109 38-39 -0.04615915401149806 2109 40-41 -0.35773297291903816 2109 42-43 -0.25055446547917626 2109 44-45 -0.3100882558237501 2109 46-47 -0.1867938444384336 2109 48-49 -0.27450682772867907 2109 50-51 -0.253615640598035 2109 52-53 -0.5342318476814611 2109 54-55 -0.3228095664797337 2109 56-57 -0.05072794513774426 2109 58-59 -0.031898595561727916 2109 60-61 -0.1419843974443893 2109 62-63 -0.002844127219184145 2109 64-65 -0.10797253508693672 2109 66-67 -0.09993580092708498 2109 68-69 0.012966207433535715 2109 70-71 -0.0703408863942343 2109 72-73 -0.36360032680548215 2109 74-75 -0.31430278151287894 2109 76 -0.49660785867470736 2108 1 0.0018590957562096833 2108 2 0.02741894246604204 2108 3 0.017317318983707253 2108 4 0.030991387237691015 2108 5 -0.0031914639804710987 2108 6 0.034925610456653544 2108 7 0.017858128583704058 2108 8 0.06629661030738276 2108 9 0.04898297804252394 2108 10-11 0.10187623490041631 2108 12-13 0.15454372280301243 2108 14-15 0.09184678566861493 2108 16-17 0.008942999518566808 2108 18-19 0.02608256743919668 2108 20-21 0.10999269865225614 2108 22-23 -0.07119118932588009 2108 24-25 0.011871742928683204 2108 26-27 -0.023032412982850303 2108 28-29 -0.027603719502565127 2108 30-31 -0.10760311297483582 2108 32-33 -0.1226803129316778 2108 34-35 -0.02220685782738485 2108 36-37 -0.09060982396418638 2108 38-39 0.013832302653554507 2108 40-41 -0.1457594130585207 2108 42-43 -0.024093914955386708 2108 44-45 -0.013861507107300497 2108 46-47 0.01800362214667217 2108 48-49 -0.025501217286809208 2108 50-51 -0.04346111146852394 2108 52-53 -0.15724138866873005 2108 54-55 -0.14356850465706827 2108 56-57 -0.13416464365428737 2108 58-59 -0.03651205222881515 2108 60-61 -0.12455667823536487 2108 62-63 -0.12577508301260565 2108 64-65 -0.11838913049445665 2108 66-67 -0.11916774109375439 2108 68-69 -0.17171655460641944 2108 70-71 -0.0335373987690204 2108 72-73 -0.04779510620632976 2108 74-75 -0.04844675405156096 2108 76 -0.19300888725167198 >>END_MODULE >>Per sequence quality scores pass #Quality Count 15 3.0 16 11.0 17 12.0 18 12.0 19 24.0 20 49.0 21 135.0 22 253.0 23 476.0 24 732.0 25 1012.0 26 1291.0 27 1885.0 28 2972.0 29 4586.0 30 7544.0 31 13685.0 32 24643.0 33 38427.0 34 67269.0 35 113827.0 36 227364.0 37 1846974.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 48.9966793955089 2.3135442757181117 42.924358720475134 5.765417608297856 2 2.9563323936144448 2.2963335664924065 5.4828220123696125 89.26451202752354 3 3.263107973615345 40.16792552734888 4.938411158318977 51.630555340716796 4 3.4618172979101525 1.945830036384714 88.96232596998283 5.63002669572231 5 3.1167956974076847 2.463213702614243 42.74094780438095 51.67904279559712 6 41.37280265988324 2.7908971071560007 6.117238501333936 49.71906173162682 7 3.4205115957684598 3.1646882141913135 44.698974071747834 48.71582611829239 8 4.398589826728529 4.406706482190528 88.7427513167255 2.4519523743554483 9 6.36825988255922 3.0606165428487166 87.70560423187968 2.865519342712391 10-11 65.84097049701978 26.143343535105174 4.065700713840725 3.9499852540343174 12-13 25.733983629003404 23.362432888857914 26.739705233670435 24.163878248468247 14-15 3.570053535929586 45.559891993238104 23.681213469738474 27.188841001093838 16-17 22.93499111417457 3.8335473693962148 46.399392143247496 26.83206937318172 18-19 27.065115124771268 44.613685446029336 5.517073448507682 22.80412598069171 20-21 23.610399605131 2.3543830267524593 50.29990198392774 23.735315384188794 22-23 25.792606279316637 2.7512062369910413 42.427797887629794 29.02838959606253 24-25 4.1459323657373455 22.855078179115463 26.513458774614502 46.48553068053269 26-27 19.408920501821786 29.7087225574179 18.348124627632494 32.534232313127816 28-29 18.771104366590656 8.20940206171548 26.430061202131917 46.58943236956195 30-31 48.16000520145879 8.142386534681066 22.14440337482885 21.553204889031296 32-33 53.57336819103973 14.772674153254354 4.170112349810002 27.483845305895922 34-35 3.6376002576931867 45.34078479134246 23.768180670801204 27.253434280163148 36-37 18.819103122320122 14.536866189073027 34.90444444255576 31.7395862460511 38-39 47.48683274505288 4.040352101363853 46.131096309429005 2.341718844154266 40-41 27.297247221426613 40.05898811228691 25.135667133834726 7.508097532451749 42-43 34.43960655893754 3.473206112903952 54.01634209960453 8.070845228553969 44-45 3.448856146517955 6.00090685564167 82.4646670513933 8.085569946447071 46-47 8.642835712944068 78.34778041344798 8.608308055546821 4.4010758180611305 48-49 12.681721716855362 28.307260879505485 33.31028656468295 25.700730838956204 50-51 37.43858326541123 22.365635355641245 31.44558058733989 8.750200791607634 52-53 45.35125995140206 28.921916924544 22.062556890955495 3.6642662330984463 54-55 14.158485559577528 58.39612763291979 4.426339439381333 23.01904736812135 56-57 25.009242788287878 22.832895487224555 10.604325369945258 41.55353635454231 58-59 2.376777696280702 23.88706205119357 50.45691245825872 23.279247794267008 60-61 8.229120010062953 41.22855141922483 24.660417833524424 25.881910737187795 62-63 4.17021858875584 21.820587067915582 55.54373942391294 18.465454919415635 64-65 33.00002634725857 37.28659782949585 26.49295465806783 3.2204211651777634 66-67 26.338292000717324 27.497018935179796 22.73749291386231 23.42719615024057 68-69 5.5253600862830226 23.42562381384217 45.00791692624382 26.041099173630982 70-71 50.15045559509533 5.831285754717222 22.065850298276466 21.952408351910986 72-73 36.31684873188944 55.118231198043844 4.111192230448422 4.4537278396182876 74-75 16.715189534528932 29.287123924755633 25.984813780126174 28.012872760589257 76 14.695438439630356 75.79719580177682 4.253892382497601 5.253473376095218 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 1.0 16 1.0 17 0.0 18 1.5 19 3.5 20 6.0 21 8.0 22 8.0 23 10.5 24 16.5 25 20.0 26 24.0 27 70.5 28 317.5 29 522.0 30 1732.5 31 3205.0 32 12985.5 33 22504.0 34 115983.5 35 130193.0 36 222422.5 37 393922.0 38 220592.0 39 31175.5 40 17902.0 41 120349.5 42 219984.0 43 124916.0 44 28387.0 45 23151.5 46 19377.0 47 37338.0 48 277161.0 49 307579.0 50 116135.0 51 184807.5 52 155863.5 53 135299.0 54 212351.0 55 121332.0 56 18336.0 57 4678.0 58 2997.0 59 2824.5 60 2845.5 61 3170.5 62 3302.0 63 3624.5 64 4213.0 65 4681.0 66 4966.5 67 5050.0 68 5079.0 69 5016.0 70 4705.0 71 4486.0 72 4185.0 73 3499.0 74 2778.5 75 2443.0 76 2072.5 77 1438.0 78 953.0 79 732.0 80 594.0 81 346.5 82 179.0 83 121.0 84 88.0 85 40.0 86 19.0 87 13.0 88 7.5 89 4.0 90 3.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 1.5 100 3.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 2.1247789167537117E-5 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 2353186.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 4.813392955739245 #Duplication Level Percentage of deduplicated Percentage of total 1 82.99815494095292 3.9950273433213725 2 5.239716151155771 0.5044162562409268 3 3.0213958374763763 0.43629496321826 4 1.994520935026696 0.38401652074927806 5 1.5242637798419232 0.3668440270289794 6 1.0551587044761133 0.30473360851873627 7 0.7682147780588412 0.258840372084225 8 0.5791343264296623 0.2230080869810664 9 0.3603773849091516 0.1561174169336497 >10 1.6608906912573342 1.547678847796646 >50 0.27803005075395826 0.9793121244983365 >100 0.3562535207549032 3.7119346805146503 >500 0.0850450502454731 2.938531047030213 >1k 0.06466967362071865 6.322832899984151 >5k 0.007972973460088602 2.471439655045734 >10k+ 0.006201201580068913 75.3989721500538 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GCCTCCCTTAGGTCACTAGTTGCCTCATCCGGCACACTGGAGTTTAACAA 510370 21.688468314871837 No Hit TCATTGTTTGGCATAGTCACGTTCAATGCTGGGTATGTGTAGTTTAAGTG 367547 15.619122330321531 No Hit GCCTCCCTTAGGTCACTAGTTGCCTCATCCGGCACCCTGGAGTTTAACAA 249484 10.601966865347661 No Hit TCATTGTTTGGCATAGTCACGTTCAGCACTGGATATGTGCTTCCTGATTT 216676 9.207771931330546 No Hit TCATTGTTTGGCATAGTCACGTTCAGTGCTGGATATTTGTATTTTAATTG 205883 8.74911715436009 No Hit GCCTCCCTTAGGTCACTAGTTGCCTCGTCAGGCACTCTGGAGTTTATCAC 200712 8.52937251878942 No Hit CTCCCTTAGGTCACTAGTTGCCTCATCCGGCACACTGGAGTTTAACAATG 17548 0.7457124086238827 No Hit CTCCCTTAGGTCACTAGTTGCCTCATCCGGCACCCTGGAGTTTAACAATG 8185 0.34782630867258263 No Hit CTCCCTTAGGTCACTAGTTGCCTCGTCAGGCACTCTGGAGTTTATCACTG 7941 0.33745738755882454 No Hit TCCCTTAGGTCACTAGTTGCCTCATCCGGCACACTGGAGTTTAACAATGA 7079 0.3008261990339905 No Hit TTGGCATAGTCACGTTCAATGCTGGGTATGTGTAGTTTAAGTGGGTCAAC 6454 0.2742664625745691 No Hit CCTCCCTTAGGTCACTAGTTGCCTCATCCGGCACACTGGAGTTTAACAAT 6218 0.2642375060874916 No Hit GCCTCCCTTAGGTCACTAGTTGCCTCGTCAGGCACTCTGGAGTTTAACAA 5746 0.24417959311333653 No Hit ATTGTTTGGCATAGTCACGTTCAATGCTGGGTATGTGTAGTTTAAGTGGG 5717 0.24294722134161942 No Hit CCCTTAGGTCACTAGTTGCCTCATCCGGCACACTGGAGTTTAACAATGAA 5576 0.23695534479637395 No Hit TGGCATAGTCACGTTCAGCACTGGATATGTGCTTCCTGATTTGGTCAACC 5043 0.21430520154377938 No Hit CATTGTTTGGCATAGTCACGTTCAATGCTGGGTATGTGTAGTTTAAGTGG 4832 0.20533863451507872 No Hit TGGCATAGTCACGTTCAATGCTGGGTATGTGTAGTTTAAGTGGGTCAACC 4207 0.1787788980556573 No Hit GCCTCCCTTAGGTCACTAGTTGCCTCGTCAGGCACTCTGGAGTTTAACAC 4103 0.1743593579088096 No Hit GCCTCCCTTAGGTCACTAGTTGCCTCATCCGGCACACTGGAGTTTATCAC 3626 0.15408896704297917 No Hit TGTTTGGCATAGTCACGTTCAATGCTGGGTATGTGTAGTTTAAGTGGGTC 3602 0.15306907316293739 No Hit TTGGCATAGTCACGTTCAGCACTGGATATGTGCTTCCTGATTTGGTCAAC 3597 0.15285659527126202 No Hit GCCTCCCTTAGGTCACTAGTTGCCTCATCCGGCACACTGGAGTTTAACAC 3499 0.14869202859442476 No Hit TCCCTTAGGTCACTAGTTGCCTCATCCGGCACCCTGGAGTTTAACAATGA 3368 0.14312510783253002 No Hit TTGGCATAGTCACGTTCAGTGCTGGATATTTGTATTTTAATTGGTGCAAC 3300 0.14023540850574498 No Hit CCTCCCTTAGGTCACTAGTTGCCTCGTCAGGCACTCTGGAGTTTATCACT 3265 0.13874806326401737 No Hit CCTTAGGTCACTAGTTGCCTCATCCGGCACACTGGAGTTTAACAATGAAA 3227 0.13713323128728455 No Hit TTGTTTGGCATAGTCACGTTCAATGCTGGGTATGTGTAGTTTAAGTGGGT 3206 0.136240824142248 No Hit TCCCTTAGGTCACTAGTTGCCTCGTCAGGCACTCTGGAGTTTATCACTGA 3155 0.13407354964715923 No Hit CCTCCCTTAGGTCACTAGTTGCCTCATCCGGCACCCTGGAGTTTAACAAT 3070 0.1304614254886779 No Hit CCCTTAGGTCACTAGTTGCCTCATCCGGCACCCTGGAGTTTAACAATGAA 3007 0.12778420405356822 No Hit TCATTGTTTGGACTGGAGTCACTCAAAACGGAACAAGTTCTGCTTGCATA 2956 0.12561692955847945 No Hit CCCTTAGGTCACTAGTTGCCTCGTCAGGCACTCTGGAGTTTATCACTGAG 2801 0.11903011491654293 No Hit TCATTGTTTGGCATAGTCACGTTCAGTGCTGGGTATGTGTAGTTTAAGTG 2788 0.11847767239818698 No Hit CATTGTTTGGCATAGTCACGTTCAGTGCTGGATATTTGTATTTTAATTGG 2666 0.11329321184130792 No Hit CATTGTTTGGCATAGTCACGTTCAGCACTGGATATGTGCTTCCTGATTTG 2551 0.10840622033277438 No Hit TCATTGTTTGGCATAGTCACGTTCAATGCTGGATATTTGTATTTTAATTG 2492 0.105898981211005 No Hit CCTTAGGTCACTAGTTGCCTCGTCAGGCACTCTGGAGTTTATCACTGAGG 2457 0.1044116359692774 No Hit GGTCACTAGTTGCCTCGTCAGGCACTCTGGAGTTTATCACTGAGGGTTTC 2422 0.10292429072754979 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0015723363983977468 0.0 0.0 2 0.0015723363983977468 0.0 0.0 3 0.0016998231334029695 0.0 0.0 4 0.0016998231334029695 0.0 0.0 5 0.0016998231334029695 0.0 0.0 6 0.0016998231334029695 0.0 0.0 7 0.0017423187117380436 0.0 0.0 8 0.0017423187117380436 0.0 0.0 9 0.0017423187117380436 0.0 0.0 10 0.0017423187117380436 0.0 0.0 11 0.0017423187117380436 0.0 0.0 12 0.0017423187117380436 0.0 0.0 13 0.0017423187117380436 0.0 0.0 14 0.0017423187117380436 0.0 0.0 15 0.0017423187117380436 0.0 0.0 16 0.0017423187117380436 0.0 0.0 17 0.0017423187117380436 0.0 0.0 18 0.0017848142900731179 0.0 0.0 19 0.0017848142900731179 0.0 0.0 20 0.0018273098684081922 0.0 0.0 21 0.0018273098684081922 0.0 0.0 22 0.0019123010250783406 0.0 0.0 23 0.0019123010250783406 0.0 0.0 24 0.0019547966034134147 0.0 0.0 25 0.0019547966034134147 0.0 0.0 26 0.0019547966034134147 0.0 0.0 27 0.001997292181748489 0.0 0.0 28 0.0020397877600835633 0.0 0.0 29 0.0020822833384186376 0.0 0.0 30 0.002124778916753712 0.0 0.0 31 0.002124778916753712 0.0 0.0 32 0.0022522656517589344 0.0 0.0 33 0.002337256808429083 0.0 0.0 34 0.0024647435434343055 0.0 0.0 35 0.002549734700104454 0.0 0.0 36 0.0028047081701148995 0.0 0.0 37 0.0031021772184604193 0.0 0.0 38 0.0033571506884708647 0.0 0.0 39 0.0036546197368163844 0.0 0.0 40 0.003952088785161904 0.0 0.0 41 0.0042070622551723495 0.0 0.0 42 0.004802000351863389 0.0 0.0 43 0.00501447824353876 0.0 0.0 44 0.005354442870219354 0.0 0.0 45 0.005736903075235022 4.249557833507423E-5 0.0 46 0.006374336750261136 4.249557833507423E-5 0.0 47 0.006756796955276804 4.249557833507423E-5 0.0 48 0.0071392571602924716 4.249557833507423E-5 0.0 49 0.007649204100313362 4.249557833507423E-5 0.0 50 0.008201646618669328 8.499115667014847E-5 0.0 51 0.00862660240202007 8.499115667014847E-5 0.0 52 0.00913654934204096 8.499115667014847E-5 0.0 53 0.01024143437875289 8.499115667014847E-5 0.0 54 0.011091345945454375 8.499115667014847E-5 0.0 55 0.01194125751215586 8.499115667014847E-5 0.0 56 0.012748673500522271 8.499115667014847E-5 0.0 57 0.013513593910553607 8.499115667014847E-5 0.0 58 0.014321009898920017 8.499115667014847E-5 0.0 59 0.015085930308951353 8.499115667014847E-5 0.0 60 0.01576585956231254 8.499115667014847E-5 0.0 61 0.017295700382375215 8.499115667014847E-5 0.0 62 0.018485576575757293 8.499115667014847E-5 0.0 63 0.020440373179170707 8.499115667014847E-5 0.0 64 0.021842727264228156 8.499115667014847E-5 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TGTCATA 70 0.0 70.00001 9 TATCACA 15 0.0022251944 70.00001 45 GAAATCT 30 1.02476406E-7 70.00001 52 TACCCTG 35 3.7161954E-9 70.00001 33 CTATCAC 45 5.456968E-12 70.00001 44 GGGTATA 15 0.0022251944 70.00001 31 GGTCCTA 60 0.0 70.00001 11 TTCACAT 15 0.0022251944 70.00001 58 ACACACT 95 0.0 70.00001 32 ATATGGT 15 0.0022251944 70.00001 47 TAAGTAG 30 1.02476406E-7 70.00001 45 TGAGTAG 15 0.0022251944 70.00001 36 TTTTTAA 95 0.0 70.00001 41 ATCGTAT 15 0.0022251944 70.00001 42 GTAATCT 15 0.0022251944 70.00001 62 TATCTTC 15 0.0022251944 70.00001 64 TATCTCT 30 1.02476406E-7 70.00001 45 TCGCTTA 15 0.0022251944 70.00001 4 TGTTAGG 15 0.0022251944 70.00001 5 ACAGAGG 15 0.0022251944 70.00001 49 >>END_MODULE