FastQCFastQC Report
Tue 25 Aug 2015
000000000-AHV5L l01n01 kokuya5.34100000033d14.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AHV5L l01n01 kokuya5.34100000033d14.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2353186
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTCCCTTAGGTCACTAGTTGCCTCATCCGGCACACTGGAGTTTAACAA51037021.688468314871837No Hit
TCATTGTTTGGCATAGTCACGTTCAATGCTGGGTATGTGTAGTTTAAGTG36754715.619122330321531No Hit
GCCTCCCTTAGGTCACTAGTTGCCTCATCCGGCACCCTGGAGTTTAACAA24948410.601966865347661No Hit
TCATTGTTTGGCATAGTCACGTTCAGCACTGGATATGTGCTTCCTGATTT2166769.207771931330546No Hit
TCATTGTTTGGCATAGTCACGTTCAGTGCTGGATATTTGTATTTTAATTG2058838.74911715436009No Hit
GCCTCCCTTAGGTCACTAGTTGCCTCGTCAGGCACTCTGGAGTTTATCAC2007128.52937251878942No Hit
CTCCCTTAGGTCACTAGTTGCCTCATCCGGCACACTGGAGTTTAACAATG175480.7457124086238827No Hit
CTCCCTTAGGTCACTAGTTGCCTCATCCGGCACCCTGGAGTTTAACAATG81850.34782630867258263No Hit
CTCCCTTAGGTCACTAGTTGCCTCGTCAGGCACTCTGGAGTTTATCACTG79410.33745738755882454No Hit
TCCCTTAGGTCACTAGTTGCCTCATCCGGCACACTGGAGTTTAACAATGA70790.3008261990339905No Hit
TTGGCATAGTCACGTTCAATGCTGGGTATGTGTAGTTTAAGTGGGTCAAC64540.2742664625745691No Hit
CCTCCCTTAGGTCACTAGTTGCCTCATCCGGCACACTGGAGTTTAACAAT62180.2642375060874916No Hit
GCCTCCCTTAGGTCACTAGTTGCCTCGTCAGGCACTCTGGAGTTTAACAA57460.24417959311333653No Hit
ATTGTTTGGCATAGTCACGTTCAATGCTGGGTATGTGTAGTTTAAGTGGG57170.24294722134161942No Hit
CCCTTAGGTCACTAGTTGCCTCATCCGGCACACTGGAGTTTAACAATGAA55760.23695534479637395No Hit
TGGCATAGTCACGTTCAGCACTGGATATGTGCTTCCTGATTTGGTCAACC50430.21430520154377938No Hit
CATTGTTTGGCATAGTCACGTTCAATGCTGGGTATGTGTAGTTTAAGTGG48320.20533863451507872No Hit
TGGCATAGTCACGTTCAATGCTGGGTATGTGTAGTTTAAGTGGGTCAACC42070.1787788980556573No Hit
GCCTCCCTTAGGTCACTAGTTGCCTCGTCAGGCACTCTGGAGTTTAACAC41030.1743593579088096No Hit
GCCTCCCTTAGGTCACTAGTTGCCTCATCCGGCACACTGGAGTTTATCAC36260.15408896704297917No Hit
TGTTTGGCATAGTCACGTTCAATGCTGGGTATGTGTAGTTTAAGTGGGTC36020.15306907316293739No Hit
TTGGCATAGTCACGTTCAGCACTGGATATGTGCTTCCTGATTTGGTCAAC35970.15285659527126202No Hit
GCCTCCCTTAGGTCACTAGTTGCCTCATCCGGCACACTGGAGTTTAACAC34990.14869202859442476No Hit
TCCCTTAGGTCACTAGTTGCCTCATCCGGCACCCTGGAGTTTAACAATGA33680.14312510783253002No Hit
TTGGCATAGTCACGTTCAGTGCTGGATATTTGTATTTTAATTGGTGCAAC33000.14023540850574498No Hit
CCTCCCTTAGGTCACTAGTTGCCTCGTCAGGCACTCTGGAGTTTATCACT32650.13874806326401737No Hit
CCTTAGGTCACTAGTTGCCTCATCCGGCACACTGGAGTTTAACAATGAAA32270.13713323128728455No Hit
TTGTTTGGCATAGTCACGTTCAATGCTGGGTATGTGTAGTTTAAGTGGGT32060.136240824142248No Hit
TCCCTTAGGTCACTAGTTGCCTCGTCAGGCACTCTGGAGTTTATCACTGA31550.13407354964715923No Hit
CCTCCCTTAGGTCACTAGTTGCCTCATCCGGCACCCTGGAGTTTAACAAT30700.1304614254886779No Hit
CCCTTAGGTCACTAGTTGCCTCATCCGGCACCCTGGAGTTTAACAATGAA30070.12778420405356822No Hit
TCATTGTTTGGACTGGAGTCACTCAAAACGGAACAAGTTCTGCTTGCATA29560.12561692955847945No Hit
CCCTTAGGTCACTAGTTGCCTCGTCAGGCACTCTGGAGTTTATCACTGAG28010.11903011491654293No Hit
TCATTGTTTGGCATAGTCACGTTCAGTGCTGGGTATGTGTAGTTTAAGTG27880.11847767239818698No Hit
CATTGTTTGGCATAGTCACGTTCAGTGCTGGATATTTGTATTTTAATTGG26660.11329321184130792No Hit
CATTGTTTGGCATAGTCACGTTCAGCACTGGATATGTGCTTCCTGATTTG25510.10840622033277438No Hit
TCATTGTTTGGCATAGTCACGTTCAATGCTGGATATTTGTATTTTAATTG24920.105898981211005No Hit
CCTTAGGTCACTAGTTGCCTCGTCAGGCACTCTGGAGTTTATCACTGAGG24570.1044116359692774No Hit
GGTCACTAGTTGCCTCGTCAGGCACTCTGGAGTTTATCACTGAGGGTTTC24220.10292429072754979No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTCATA700.070.000019
TATCACA150.002225194470.0000145
GAAATCT301.02476406E-770.0000152
TACCCTG353.7161954E-970.0000133
CTATCAC455.456968E-1270.0000144
GGGTATA150.002225194470.0000131
GGTCCTA600.070.0000111
TTCACAT150.002225194470.0000158
ACACACT950.070.0000132
ATATGGT150.002225194470.0000147
TAAGTAG301.02476406E-770.0000145
TGAGTAG150.002225194470.0000136
TTTTTAA950.070.0000141
ATCGTAT150.002225194470.0000142
GTAATCT150.002225194470.0000162
TATCTTC150.002225194470.0000164
TATCTCT301.02476406E-770.0000145
TCGCTTA150.002225194470.000014
TGTTAGG150.002225194470.000015
ACAGAGG150.002225194470.0000149
Produced by FastQC (version 0.11.2)