##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename 000000000-AHV5L l01n01 kokuya4.34100000033d07.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2459974 Sequences flagged as poor quality 0 Sequence length 76 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 33.64178849044746 34.0 34.0 34.0 34.0 34.0 2 33.8271721571041 34.0 34.0 34.0 34.0 34.0 3 33.82354406997798 34.0 34.0 34.0 34.0 34.0 4 33.87166531028377 34.0 34.0 34.0 34.0 34.0 5 33.898418845077224 34.0 34.0 34.0 34.0 34.0 6 37.72441741254176 38.0 38.0 38.0 38.0 38.0 7 37.737161449673856 38.0 38.0 38.0 38.0 38.0 8 37.77360045268771 38.0 38.0 38.0 38.0 38.0 9 37.737072830851055 38.0 38.0 38.0 38.0 38.0 10-11 37.534174548186286 38.0 38.0 38.0 37.0 38.0 12-13 37.51304099149016 38.0 38.0 38.0 37.0 38.0 14-15 37.512334479957914 38.0 38.0 38.0 37.0 38.0 16-17 37.716599443733955 38.0 38.0 38.0 37.5 38.0 18-19 37.680698861044874 38.0 38.0 38.0 38.0 38.0 20-21 37.59742277763911 38.0 38.0 38.0 37.0 38.0 22-23 37.583378320258674 38.0 38.0 38.0 37.0 38.0 24-25 37.691787392874886 38.0 38.0 38.0 37.5 38.0 26-27 37.08705213957546 38.0 38.0 38.0 36.5 38.0 28-29 37.433670640421404 38.0 38.0 38.0 37.0 38.0 30-31 37.34096376628371 38.0 38.0 38.0 37.0 38.0 32-33 37.391094580674434 38.0 38.0 38.0 37.0 38.0 34-35 37.50554213174611 38.0 38.0 38.0 37.0 38.0 36-37 37.05127228986973 38.0 38.0 38.0 36.5 38.0 38-39 37.6112525986047 38.0 38.0 38.0 37.0 38.0 40-41 37.07889107771058 38.0 38.0 38.0 36.5 38.0 42-43 37.132338187314176 38.0 38.0 38.0 37.0 38.0 44-45 37.25480757113693 38.0 38.0 38.0 37.0 38.0 46-47 36.99602597425826 38.0 38.0 38.0 36.5 38.0 48-49 37.29532263349125 38.0 38.0 38.0 37.0 38.0 50-51 37.03239485457976 38.0 38.0 38.0 36.5 38.0 52-53 37.0898054207077 38.0 38.0 38.0 36.5 38.0 54-55 36.927569356424094 38.0 38.0 38.0 36.0 38.0 56-57 37.09473189554036 38.0 38.0 38.0 36.5 38.0 58-59 37.283193846764235 38.0 38.0 38.0 36.5 38.0 60-61 37.21316932617987 38.0 38.0 38.0 37.0 38.0 62-63 36.990364532308064 38.0 38.0 38.0 36.5 38.0 64-65 37.218859833477914 38.0 38.0 38.0 37.0 38.0 66-67 37.36297599080315 38.0 38.0 38.0 37.0 38.0 68-69 37.45301799937723 38.0 38.0 38.0 37.0 38.0 70-71 37.406483157952074 38.0 38.0 38.0 37.0 38.0 72-73 36.92846834966548 38.0 38.0 38.0 36.0 38.0 74-75 37.07580649226374 38.0 38.0 38.0 36.0 38.0 76 36.10531086913927 38.0 37.0 38.0 34.0 38.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.013909520263275965 1101 2 -0.005528033016950928 1101 3 0.048971514963952245 1101 4 -0.010098555186594638 1101 5 0.01753253441105329 1101 6 -0.07237037568783222 1101 7 -0.09895013587136958 1101 8 -0.07510428094214916 1101 9 -0.4372602100674641 1101 10-11 -0.5508229317619495 1101 12-13 -0.8010494307044098 1101 14-15 -0.3637946197290276 1101 16-17 -0.07020704358743046 1101 18-19 0.012375438103198633 1101 20-21 0.12842427298505044 1101 22-23 0.06703222016107446 1101 24-25 0.05987196232543113 1101 26-27 0.0985826799070324 1101 28-29 0.10555509611042169 1101 30-31 0.11101115075378942 1101 32-33 0.08862251290836554 1101 34-35 0.07945874511262474 1101 36-37 0.11966001205784238 1101 38-39 0.03502640592235906 1101 40-41 0.16285349036133567 1101 42-43 0.10839350106476786 1101 44-45 0.1298936129500774 1101 46-47 0.198263851744521 1101 48-49 0.0954962794429477 1101 50-51 0.18874270093011347 1101 52-53 0.21398530160712426 1101 54-55 0.25273714394935354 1101 56-57 0.15235754503571997 1101 58-59 0.14142817753518244 1101 60-61 0.14702720178448914 1101 62-63 0.11662956830798521 1101 64-65 0.14709892110860068 1101 66-67 0.16784013126450503 1101 68-69 0.08938179464012563 1101 70-71 0.0907227167142679 1101 72-73 0.153978468995966 1101 74-75 0.17190063164948555 1101 76 0.2526159331912794 1102 1 0.024709515444051533 1102 2 0.0075761937520439915 1102 3 0.04302563831517148 1102 4 0.00997049639544656 1102 5 0.03726828226705692 1102 6 -0.020457896588752078 1102 7 0.03459455668730271 1102 8 -0.04048880746074701 1102 9 -0.21008705595231447 1102 10-11 -0.17591839703189294 1102 12-13 0.03862052428432605 1102 14-15 0.16939313194709626 1102 16-17 0.05921209936954597 1102 18-19 0.0738206489007851 1102 20-21 0.1485046163684629 1102 22-23 0.10091247480605858 1102 24-25 0.07154836297690537 1102 26-27 0.08728280737139471 1102 28-29 0.06519772859076056 1102 30-31 0.11343521104250698 1102 32-33 0.15042147263356043 1102 34-35 0.12159521026139686 1102 36-37 0.14734334957844908 1102 38-39 0.07711663466503893 1102 40-41 0.10394594893489995 1102 42-43 0.10011434186533563 1102 44-45 0.12809538706158463 1102 46-47 0.18971797915348532 1102 48-49 0.06644098805528387 1102 50-51 0.15171972673012846 1102 52-53 0.23653488552380963 1102 54-55 0.2637672258819279 1102 56-57 0.22155717224459437 1102 58-59 0.17090581619576994 1102 60-61 0.11364215652105258 1102 62-63 0.19701438976947827 1102 64-65 0.14345910151246954 1102 66-67 0.19475514384743775 1102 68-69 0.05922883845435223 1102 70-71 0.07463832799736281 1102 72-73 0.21788599660103358 1102 74-75 0.20227853720860622 1102 76 0.5492974527057797 1103 1 -0.003183371548587388 1103 2 0.020253356766261277 1103 3 0.01894924790192931 1103 4 -0.004747479221059336 1103 5 0.009842537834373388 1103 6 0.06455892096052196 1103 7 0.05213069056448205 1103 8 0.04123155144572621 1103 9 0.07454247046069895 1103 10-11 0.17016385175939064 1103 12-13 0.20611755364451767 1103 14-15 0.1396940073147448 1103 16-17 0.04686705101811128 1103 18-19 0.05427423001423648 1103 20-21 0.15069691986956713 1103 22-23 0.09994036258340344 1103 24-25 0.04106004401752017 1103 26-27 0.02729894189510418 1103 28-29 -0.009570785703502338 1103 30-31 0.0424581012625751 1103 32-33 0.07343517172795089 1103 34-35 0.12299726361352015 1103 36-37 -0.015607140974680078 1103 38-39 0.053869351255841025 1103 40-41 -0.0016157561680287813 1103 42-43 0.01313820552717715 1103 44-45 0.005170012036224136 1103 46-47 0.034131403577752906 1103 48-49 -0.08613991006598809 1103 50-51 0.05265935613402206 1103 52-53 0.013163705969937212 1103 54-55 0.08145524170310381 1103 56-57 0.133466843938713 1103 58-59 0.1919338921961753 1103 60-61 0.08517350765510656 1103 62-63 0.09601464368813595 1103 64-65 0.1530881195667675 1103 66-67 0.15585012506944196 1103 68-69 0.11211493429586739 1103 70-71 0.13010984504927592 1103 72-73 0.1663287765255106 1103 74-75 0.18411555792409473 1103 76 0.4446206306829481 2116 1 0.012015337276451987 2116 2 0.011600025572384709 2116 3 -0.0385227290586414 2116 4 1.8580521044953002E-4 2116 5 0.010262753183013729 2116 6 0.015914592148526197 2116 7 0.03283485604160319 2116 8 0.053378515329917775 2116 9 0.08437111262227148 2116 10-11 0.17708224475087064 2116 12-13 0.14138271755247445 2116 14-15 0.11091649743192278 2116 16-17 0.025690562655562132 2116 18-19 0.04582631775412693 2116 20-21 0.11484279804984254 2116 22-23 0.12020155669100774 2116 24-25 0.012518273590764295 2116 26-27 0.061779627409812576 2116 28-29 -0.009009282972570531 2116 30-31 0.1227090218186433 2116 32-33 -0.02354517515549759 2116 34-35 0.09021369379400568 2116 36-37 0.12756975166149687 2116 38-39 0.06570175274540446 2116 40-41 0.08054150210654853 2116 42-43 0.03697784643217261 2116 44-45 0.01371706511068993 2116 46-47 0.14346606087249114 2116 48-49 0.08624049058169447 2116 50-51 0.14654945830688604 2116 52-53 0.13776000540750033 2116 54-55 0.21572951843882038 2116 56-57 0.14733546126037567 2116 58-59 0.1442072541065187 2116 60-61 0.12374293123176017 2116 62-63 0.08895152490462976 2116 64-65 0.09163639171377014 2116 66-67 0.1539532690262817 2116 68-69 -0.07291615726376932 2116 70-71 0.054717762144008475 2116 72-73 0.23979587094423493 2116 74-75 0.08911266132437135 2116 76 0.3786379644358391 1117 1 0.010526846185193506 1117 2 -0.016556249698751913 1117 3 7.989890892332596E-4 1117 4 0.02571390840818566 1117 5 0.01942274648835962 1117 6 0.049584517678347595 1117 7 0.08510712709781387 1117 8 0.07149051935088124 1117 9 0.0967512124059482 1117 10-11 0.18046320013602468 1117 12-13 0.2169122806430437 1117 14-15 0.18591593693735575 1117 16-17 0.08819178140538497 1117 18-19 0.037891813239141925 1117 20-21 0.15170604363144946 1117 22-23 0.07330205623505037 1117 24-25 -0.014260749209114465 1117 26-27 0.13890082562728168 1117 28-29 0.1485357038857842 1117 30-31 0.044052172757481856 1117 32-33 0.09818774110036088 1117 34-35 0.1490139538634807 1117 36-37 0.19826701733435925 1117 38-39 0.047809241004991065 1117 40-41 0.1979698330997124 1117 42-43 -0.21666974795120808 1117 44-45 0.032173773292193175 1117 46-47 -0.08627263395202789 1117 48-49 -0.038588089323205566 1117 50-51 -0.1014169761499275 1117 52-53 0.006007101654581959 1117 54-55 -0.007832421946453394 1117 56-57 0.11957399575738492 1117 58-59 0.10301815053806962 1117 60-61 0.1660288053608454 1117 62-63 0.1488640408265809 1117 64-65 0.17254071972249818 1117 66-67 0.14903349535138233 1117 68-69 0.1861074273049539 1117 70-71 0.1468800928420677 1117 72-73 0.1461380234206544 1117 74-75 0.23960533605139034 1117 76 0.3898799995473041 2117 1 -0.03664269152898214 2117 2 0.0026542758727643445 2117 3 -0.016028488633892835 2117 4 0.026921874681185898 2117 5 -0.0019042783309686229 2117 6 -0.03433833620946558 2117 7 -0.002520796047122076 2117 8 -0.029054816408567774 2117 9 -0.2262896990889658 2117 10-11 -0.34808150158382745 2117 12-13 -0.8220508923523226 2117 14-15 -1.2083159996833714 2117 16-17 -0.1032819287058544 2117 18-19 0.048123235120506536 2117 20-21 0.16353409600116464 2117 22-23 0.12947595894930686 2117 24-25 0.030597012260876966 2117 26-27 0.14113668938539803 2117 28-29 0.12454523710562881 2117 30-31 0.13202435677705893 2117 32-33 0.13283707940830425 2117 34-35 0.15651079073609964 2117 36-37 0.21981440048119083 2117 38-39 0.08720959535754247 2117 40-41 0.2596936520338389 2117 42-43 0.20870370162786145 2117 44-45 0.17965137043124457 2117 46-47 0.07928389777423916 2117 48-49 0.19152664458972168 2117 50-51 0.25573405717263853 2117 52-53 0.26972092045244267 2117 54-55 0.27990738373300417 2117 56-57 0.15794987222199808 2117 58-59 0.20221611358945069 2117 60-61 0.20925162656964602 2117 62-63 0.1902958158777679 2117 64-65 0.19392798601680994 2117 66-67 0.20258657251317658 2117 68-69 0.21745826846157712 2117 70-71 0.1860820591361545 2117 72-73 0.18208301624336087 2117 74-75 0.22304106446544125 2117 76 0.39201899614493385 1116 1 0.07950250654559454 1116 2 0.0033810990692373366 1116 3 0.01428449508584606 1116 4 0.01974513023372282 1116 5 -0.03651471381565585 1116 6 -0.03820773511186104 1116 7 0.02220826961917055 1116 8 -0.054202140029453005 1116 9 -0.19883134129400304 1116 10-11 -0.2441373485592706 1116 12-13 -0.02729293222036233 1116 14-15 0.08283484456590173 1116 16-17 0.02934463791532238 1116 18-19 0.04651751124296055 1116 20-21 0.15149087204670053 1116 22-23 0.09492383137222049 1116 24-25 0.042530671708227885 1116 26-27 -0.007433679694948125 1116 28-29 0.05539769974036801 1116 30-31 0.03249706621475923 1116 32-33 0.08176166005291918 1116 34-35 0.09954332398442745 1116 36-37 0.03420881773237738 1116 38-39 0.017844416163029564 1116 40-41 0.14287740019947393 1116 42-43 0.12830435348667635 1116 44-45 0.13366999552565773 1116 46-47 0.017771747575537233 1116 48-49 0.1036092943307807 1116 50-51 0.12960136068267758 1116 52-53 0.15360785070161853 1116 54-55 0.07821628005303438 1116 56-57 0.13919946965640406 1116 58-59 0.09072516346901693 1116 60-61 0.11411483939792078 1116 62-63 -0.05507675677993973 1116 64-65 0.10798943793601978 1116 66-67 0.10621547309168733 1116 68-69 0.18658989807580184 1116 70-71 0.14422328919607708 1116 72-73 0.062391881999168675 1116 74-75 0.1782931043704039 1116 76 0.22836762266293675 2118 1 -0.1197266143780027 2118 2 0.025945544130479448 2118 3 -0.024751377390337836 2118 4 -0.005157064276943402 2118 5 0.011392215670220196 2118 6 0.04045133841242432 2118 7 0.03333385054578031 2118 8 -0.013265297263345133 2118 9 0.08053054397289117 2118 10-11 0.15694125847247875 2118 12-13 0.2090730527816902 2118 14-15 0.16030459838354005 2118 16-17 0.060873897782187214 2118 18-19 0.07091622225912175 2118 20-21 0.1622827247833527 2118 22-23 0.10931203903707143 2118 24-25 0.07057179049068196 2118 26-27 0.09196827360623416 2118 28-29 0.1262409044892152 2118 30-31 0.12474988431802814 2118 32-33 0.08210148043445287 2118 34-35 0.1540550152098561 2118 36-37 0.16910687368306299 2118 38-39 0.07432770579779202 2118 40-41 0.11434942237753631 2118 42-43 0.13638383862007686 2118 44-45 0.24166658555048315 2118 46-47 0.11145623070161292 2118 48-49 0.08564703535440543 2118 50-51 0.1923524828850205 2118 52-53 0.1855281466623495 2118 54-55 0.21941562399627657 2118 56-57 0.16152745507810096 2118 58-59 0.11494079896514364 2118 60-61 0.10419096907610736 2118 62-63 0.13106406495138856 2118 64-65 0.11230921480072453 2118 66-67 0.20517569087410692 2118 68-69 0.15044998501034001 2118 70-71 0.13166738559395696 2118 72-73 0.19728301969839634 2118 74-75 0.25966175754586374 2118 76 0.3994516445171783 1119 1 -0.054153975109713315 1119 2 -0.0035876254026945276 1119 3 0.023515418485153816 1119 4 0.010166669959829733 1119 5 0.012947528747275783 1119 6 0.08454061967562154 1119 7 0.08344774761619789 1119 8 0.035500453241539276 1119 9 0.09342405860553527 1119 10-11 0.22593943225326996 1119 12-13 0.22961576799832528 1119 14-15 0.15105287771877585 1119 16-17 0.06591887924021478 1119 18-19 0.07738112731099278 1119 20-21 0.12434027358002453 1119 22-23 0.07404290061575836 1119 24-25 0.05413408796602681 1119 26-27 0.13659224540412396 1119 28-29 0.06058439394139725 1119 30-31 0.14735134376731196 1119 32-33 0.1911297817453601 1119 34-35 0.19840148053457796 1119 36-37 0.12830024689817776 1119 38-39 0.05275240096161582 1119 40-41 0.11903038086283857 1119 42-43 0.17035323291555926 1119 44-45 0.1825777748312376 1119 46-47 0.06314847206776619 1119 48-49 0.07983228214834526 1119 50-51 0.13752457479624525 1119 52-53 0.19467267232862184 1119 54-55 0.15637688932711313 1119 56-57 0.18283049301157206 1119 58-59 0.1446355558338155 1119 60-61 0.19057600883071046 1119 62-63 0.16081479111515762 1119 64-65 0.1805416991201625 1119 66-67 0.18552165513311536 1119 68-69 0.08806272479699118 1119 70-71 0.13054164098995358 1119 72-73 0.226675992569632 1119 74-75 0.21427391487541314 1119 76 0.27441880817999476 2119 1 0.029081779040737388 2119 2 0.027516581792220052 2119 3 0.002141903854592897 2119 4 -0.0015962819314339072 2119 5 0.028503608899470123 2119 6 0.10207947314020771 2119 7 0.07366249419604287 2119 8 0.06373240126102075 2119 9 0.14052305462014658 2119 10-11 0.2070549757851765 2119 12-13 0.2570358676520428 2119 14-15 0.21196132480471164 2119 16-17 0.04140571684403227 2119 18-19 0.08231007322100226 2119 20-21 0.13238291234601007 2119 22-23 0.10570200762042248 2119 24-25 -0.052907165948987256 2119 26-27 0.04085883615857 2119 28-29 0.012162303485510506 2119 30-31 0.10473531358083932 2119 32-33 0.08555068807162058 2119 34-35 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-0.0019167177589949347 2104 44-45 0.02386742301991518 2104 46-47 0.015208054598062404 2104 48-49 -0.007853994923486596 2104 50-51 -0.062014836564941334 2104 52-53 -0.0812597746710253 2104 54-55 -0.028869901252058128 2104 56-57 0.0053575240114795974 2104 58-59 0.0786635831424718 2104 60-61 -0.046361240050480035 2104 62-63 -0.041015150412953005 2104 64-65 -0.2117681039046957 2104 66-67 -0.15687928564367581 2104 68-69 -0.010516310253471772 2104 70-71 -0.11290395401870512 2104 72-73 -0.15993100445777486 2104 74-75 -0.11244349276186938 2104 76 -0.375434522104527 2111 1 0.06581790038553947 2111 2 -0.0077816300814390615 2111 3 0.03759049128007774 2111 4 0.019539439099986566 2111 5 -0.04018805785798918 2111 6 -0.13548341270185915 2111 7 -0.05295993233687568 2111 8 -0.09532144933744746 2111 9 -0.04914638866634391 2111 10-11 -0.2932966172591094 2111 12-13 -0.45218292299403373 2111 14-15 -0.3050083196897049 2111 16-17 -0.042932604924672546 2111 18-19 -0.03755825953222569 2111 20-21 0.11187074573582123 2111 22-23 -0.045482069639447786 2111 24-25 0.0018437266997821666 2111 26-27 -0.014025062638445718 2111 28-29 0.008706580603906389 2111 30-31 -0.022246255833415773 2111 32-33 -0.028284440880554484 2111 34-35 0.01978389810660275 2111 36-37 0.06171952673108194 2111 38-39 0.03200829610855038 2111 40-41 -0.07453433506547213 2111 42-43 0.0080509500762318 2111 44-45 0.044327206545936804 2111 46-47 0.061879676075683676 2111 48-49 0.04072907302171558 2111 50-51 -0.048861769325640125 2111 52-53 -0.11997724973173263 2111 54-55 0.03235868054346014 2111 56-57 -0.1417085040151278 2111 58-59 -0.28924028003640245 2111 60-61 -0.05122006840404936 2111 62-63 -0.004987076597956275 2111 64-65 -0.05429159991499688 2111 66-67 -0.04791935082057108 2111 68-69 -0.13365381614911342 2111 70-71 -0.07935663534927073 2111 72-73 -0.03859982390437011 2111 74-75 0.03240393098372607 2111 76 -0.19700586711088874 2110 1 0.05799950918825658 2110 2 0.005973144834939603 2110 3 -0.023336788752203574 2110 4 0.0018679291848897606 2110 5 0.01792639303340593 2110 6 -0.11338144483627133 2110 7 0.0063713932730919964 2110 8 -0.021519050284794616 2110 9 -0.08419673764618096 2110 10-11 -0.3197397472644852 2110 12-13 -0.3826647241924661 2110 14-15 -0.11235361852842374 2110 16-17 -0.038609097639280776 2110 18-19 -0.07365379809944983 2110 20-21 -0.017774834949882745 2110 22-23 -0.00730300286927843 2110 24-25 -0.05945106299959235 2110 26-27 -0.06534320515766723 2110 28-29 -0.04555586975486392 2110 30-31 -0.09895495482209782 2110 32-33 -0.11450729648640845 2110 34-35 0.0027313185302872967 2110 36-37 -0.061408570141352925 2110 38-39 0.03205173371255654 2110 40-41 -0.15173996823872216 2110 42-43 0.004620312068439603 2110 44-45 -0.11511410547579004 2110 46-47 -0.02153624644441976 2110 48-49 -0.04516827814608604 2110 50-51 -0.03342829534862801 2110 52-53 -0.16227702613910822 2110 54-55 -0.12924379662580776 2110 56-57 -0.1913847339722068 2110 58-59 -0.09328030143719701 2110 60-61 -0.15858793214535183 2110 62-63 -0.25970168887469924 2110 64-65 -0.2087560084835829 2110 66-67 -0.22093812306069793 2110 68-69 -0.2465862346101133 2110 70-71 -0.20393811536703765 2110 72-73 -0.27996722191283396 2110 74-75 -0.157661435948107 2110 76 -0.38351852215492954 2109 1 0.06666877965710682 2109 2 0.04245982892656741 2109 3 -4.217351950543957E-4 2109 4 0.005558636985725229 2109 5 0.013801325986584345 2109 6 -0.1730604152301254 2109 7 -0.053126888263356875 2109 8 -0.08160491257085312 2109 9 -0.1970355391817762 2109 10-11 -0.39629895090624956 2109 12-13 -0.46513179160745466 2109 14-15 -0.10802598802077057 2109 16-17 -0.06779600704705047 2109 18-19 -0.05076756871716981 2109 20-21 0.0444684500873862 2109 22-23 -0.009275048560127175 2109 24-25 -0.02939433199327368 2109 26-27 -0.08797155028424442 2109 28-29 -0.1742696876384997 2109 30-31 -0.03843768961568372 2109 32-33 -0.10358049634443489 2109 34-35 -0.1326482414696244 2109 36-37 -0.1224968584676418 2109 38-39 -0.02720671039178768 2109 40-41 -0.32438966068378505 2109 42-43 -0.1829150976527032 2109 44-45 -0.2969945275755492 2109 46-47 -0.11609049387246273 2109 48-49 -0.238950834130506 2109 50-51 -0.21335200493896167 2109 52-53 -0.48936381256373096 2109 54-55 -0.21415125569129145 2109 56-57 -0.0326217965176383 2109 58-59 0.02291064242844243 2109 60-61 -0.08939435865012513 2109 62-63 0.022421491683374484 2109 64-65 -0.020637750340064542 2109 66-67 -0.03866236962566205 2109 68-69 0.07058464957128763 2109 70-71 -0.04907748361702602 2109 72-73 -0.2720288478001507 2109 74-75 -0.29271075893188936 2109 76 -0.5134883040945866 2108 1 0.021137098294218504 2108 2 0.028038620691638982 2108 3 0.01203757163265351 2108 4 0.026257443201387787 2108 5 -0.012030379560883375 2108 6 0.018108217665051995 2108 7 -0.007926594969987377 2108 8 0.03888659531408223 2108 9 0.030123695693703212 2108 10-11 0.0952870319246415 2108 12-13 0.10670665121315892 2108 14-15 0.09363194527471563 2108 16-17 0.025503180230622036 2108 18-19 0.028450002931201368 2108 20-21 0.11432959970256462 2108 22-23 -0.06030084999071761 2108 24-25 0.04167553819017655 2108 26-27 -0.009108122682924602 2108 28-29 0.02820489554895289 2108 30-31 -0.07591667406129687 2108 32-33 -0.07590113368517848 2108 34-35 -0.03829000559261431 2108 36-37 -0.04786944834645368 2108 38-39 0.007624928988640534 2108 40-41 -0.04171821374329454 2108 42-43 0.07927335996703988 2108 44-45 0.06504156451057241 2108 46-47 0.08882141152424339 2108 48-49 -0.008274042609222931 2108 50-51 0.05315889422335118 2108 52-53 -0.06774311724720405 2108 54-55 -0.03753486034782583 2108 56-57 -0.1041207241339066 2108 58-59 -0.06522193172950352 2108 60-61 -0.1071149403680991 2108 62-63 -0.08446327956531974 2108 64-65 -0.10984456586369618 2108 66-67 -0.13160584271593478 2108 68-69 -0.21386439526148848 2108 70-71 -0.05211815989370905 2108 72-73 -0.005974428239902352 2108 74-75 -0.04429850253141865 2108 76 -0.10265732567690122 >>END_MODULE >>Per sequence quality scores pass #Quality Count 14 4.0 15 15.0 16 19.0 17 25.0 18 28.0 19 30.0 20 52.0 21 121.0 22 278.0 23 486.0 24 715.0 25 960.0 26 1307.0 27 1943.0 28 3025.0 29 4814.0 30 7830.0 31 14944.0 32 27941.0 33 41183.0 34 73899.0 35 118209.0 36 228733.0 37 1933413.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 51.49188568659668 1.7779049697273224 43.20277368785199 3.5274356558240045 2 2.6262879201162286 2.112217446200651 3.363612786151399 91.89788184753172 3 2.6236456157666708 41.43291758368178 3.8965045972030596 52.0469322033485 4 2.6009624491966177 1.718473447280337 92.55000256100267 3.130561542520368 5 2.7864928653717476 2.0720137692512197 42.929234211418496 52.21225915395854 6 42.53044950881595 1.8348161403331904 4.010611494267826 51.624122856583035 7 2.6077511388331747 2.199250886391482 43.98761938134306 51.20537859343229 8 3.2668231452852754 2.290227457688577 92.45951379973934 1.9834355972868005 9 3.81138174631114 2.32461806506898 91.5442195730524 2.3197806155674816 10-11 67.61380404833547 27.15729922348773 2.3241505804532894 2.904746147723513 12-13 27.05205420870302 22.611621098434373 27.47327817285874 22.86304652000387 14-15 2.777773261018206 46.8911866548183 22.868270152448765 27.462769931714725 16-17 22.383366653468695 2.7052318439137975 47.150396711509956 27.761004791107545 18-19 27.436448515309515 46.633033519866466 3.1924321151361763 22.73808584968784 20-21 22.762517002212217 1.964654910986864 52.57494591406251 22.697882172738414 22-23 27.069107234466706 2.239657817521649 42.188860532672294 28.50237441533935 24-25 3.1246468458609726 22.743858268420723 27.045468773247194 47.08602611247111 26-27 19.80220116147569 30.11104995418651 16.47173913220221 33.61500975213559 28-29 17.069590979416855 8.15386666688347 27.493867821367218 47.28267453233246 30-31 48.91220395012305 8.471207419265408 22.068607229182096 20.547981401429446 32-33 56.03079544743156 13.58264355639531 3.16373262481636 27.222828371356773 34-35 2.9508645213323392 46.73777039919934 23.141484422193077 27.169880657275243 36-37 18.588936305830874 15.195871988890937 34.1798734458169 32.03531825946128 38-39 47.63647908473829 2.646958870297003 47.52712833550273 2.1894337094619702 40-41 27.804765416219844 40.47624080579714 23.45780890367134 8.26118487431168 42-43 36.3500183335271 2.1455308064231575 52.97616966683387 8.528281193215863 44-45 2.8976932276519993 3.4689391026084015 85.44641122223243 8.186956447507168 46-47 9.151173955497091 79.28532984494959 8.84643496232074 2.7170612372325884 48-49 12.389297610462549 27.83750966473629 32.845164217182784 26.92802850761837 50-51 36.696424433754174 21.113800389760218 33.21360307060156 8.976172105884046 52-53 46.64520844529251 28.187025553928617 22.127307036578436 3.0404589642004343 54-55 15.052110306856903 59.81012400944075 2.5649051575341852 22.572860526168164 56-57 26.42570612534929 22.608267404452242 9.144507218368975 41.82151925182949 58-59 2.0769325204250126 22.958535334113286 52.443704689561756 22.52082745589994 60-61 8.396104999483734 41.103584834636465 23.647180823862364 26.853129342017436 62-63 2.419578418308486 21.443620948839296 56.96212642897852 19.1746742038737 64-65 33.81533707266825 36.196378498309336 27.462790257132795 2.5254941718896218 66-67 26.884145929997633 27.739175292096586 22.512798915760897 22.863879862144884 68-69 2.8103142553539184 23.074024359607055 46.7266727209312 27.38898866410783 70-71 51.80136050218417 4.040347580909391 22.180762886111804 21.977529030794635 72-73 39.434481827856715 55.40357337110067 2.649987357589958 2.5119574434526544 74-75 16.029437709504247 29.56553605851119 26.838169834315323 27.56685639766924 76 12.665215160810641 81.25366365660776 2.2328284770489444 3.8482927055326597 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1.0 1 1.0 2 0.5 3 1.5 4 3.0 5 4.0 6 4.0 7 10.5 8 18.0 9 16.0 10 18.5 11 18.5 12 14.0 13 25.5 14 41.5 15 59.0 16 87.5 17 103.0 18 118.5 19 168.0 20 228.0 21 254.0 22 285.0 23 351.5 24 452.0 25 517.0 26 536.5 27 625.0 28 857.5 29 1021.0 30 1454.0 31 2597.0 32 9330.0 33 15353.0 34 103975.0 35 118447.5 36 249274.5 37 454251.0 38 247603.0 39 28642.5 40 18308.5 41 148677.5 42 277068.0 43 151178.0 44 20438.0 45 13011.0 46 10434.0 47 19507.0 48 309887.0 49 330603.5 50 70013.0 51 155220.0 52 139095.5 53 153835.5 54 269907.0 55 149622.0 56 18092.0 57 5026.5 58 3206.0 59 2874.0 60 2542.5 61 2640.5 62 2738.0 63 2850.5 64 3157.0 65 3506.0 66 3764.5 67 3868.0 68 3908.0 69 3909.0 70 3892.5 71 3915.0 72 3853.0 73 3745.5 74 3521.5 75 3343.0 76 3185.5 77 2870.0 78 2530.5 79 2349.0 80 2150.0 81 1789.0 82 1476.0 83 1325.0 84 1173.0 85 916.0 86 696.0 87 581.0 88 483.0 89 315.0 90 213.5 91 135.5 92 89.0 93 68.5 94 34.5 95 18.0 96 15.0 97 11.5 98 6.0 99 2.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 2459974.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 5.286133532402035 #Duplication Level Percentage of deduplicated Percentage of total 1 87.93063601055293 4.648130835408217 2 4.479034698471185 0.4735355102476154 3 2.254223990514762 0.35748387077415633 4 1.3747809756466318 0.2906910326029618 5 1.0095083379518146 0.26681979382432663 6 0.6215754629914043 0.19714385387023095 7 0.38445064780996563 0.1422580222659361 8 0.26276193937900605 0.11111957590322838 9 0.16288910028020348 0.07749481815485831 >10 0.9078090953446647 1.062203219104474 >50 0.24538438990396114 0.9186977666915082 >100 0.26228703404666504 3.015163094956532 >500 0.05232915855432349 1.9622858016316271 >1k 0.04386414761021339 4.480534952601052 >5k 0.0038477322465099465 1.378050371374606 >10k+ 0.0046172786958119365 80.6183874805887 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GCCTCCCTTAGGTCACTAGTTGCCTCATCCGGCACACTGGAGTTTAACAA 605917 24.63103268571131 No Hit TCATTGTTTGGCATAGTCACGTTCAATGCTGGGTATGTGTAGTTTAAGTG 415304 16.882454855213915 No Hit TCATTGTTTGGCATAGTCACGTTCAGCACTGGATATGTGCTTCCTGATTT 272366 11.071905638027069 No Hit GCCTCCCTTAGGTCACTAGTTGCCTCGTCAGGCACTCTGGAGTTTATCAC 266005 10.813325669295692 No Hit GCCTCCCTTAGGTCACTAGTTGCCTCATCCGGCACCCTGGAGTTTAACAA 235788 9.584979353440321 No Hit TCATTGTTTGGCATAGTCACGTTCAGTGCTGGATATTTGTATTTTAATTG 186426 7.578372779549703 No Hit ATTGTTTGGCATAGTCACGTTCAATGCTGGGTATGTGTAGTTTAAGTGGG 8805 0.35793061227476386 No Hit CATTGTTTGGCATAGTCACGTTCAATGCTGGGTATGTGTAGTTTAAGTGG 7209 0.29305187778407416 No Hit TGGCATAGTCACGTTCAATGCTGGGTATGTGTAGTTTAAGTGGGTCAACC 6757 0.2746776998456081 No Hit TCATTGTTTGGCATAGTCACTCAAAACGGAACAAGTTCTGCTTGCATAAG 5818 0.23650656470352938 No Hit GCCTCCCTTAGGTCACTAGTTGCCTCGTCAGGCACTCTGGAGTTTAACAC 5287 0.2149209707094465 No Hit CTCCCTTAGGTCACTAGTTGCCTCATCCGGCACACTGGAGTTTAACAATG 4594 0.18674994125954175 No Hit GCCTCCCTTAGGTCACTAGTTGCCTCATCCGGCACACTGGAGTTTAACAC 4262 0.17325386365872159 No Hit TTGTTTGGCATAGTCACGTTCAATGCTGGGTATGTGTAGTTTAAGTGGGT 4250 0.172766053624957 No Hit CCTCCCTTAGGTCACTAGTTGCCTCATCCGGCACACTGGAGTTTAACAAT 4061 0.1650830455931648 No Hit TCATTGTTTGGACTGGAGTCACTCAAAACGGAACAAGTTCTGCTTGCATA 4000 0.16260334458819484 No Hit TGTTTGGCATAGTCACGTTCAATGCTGGGTATGTGTAGTTTAAGTGGGTC 3886 0.15796914926743127 No Hit CATTGTTTGGCATAGTCACGTTCAGCACTGGATATGTGCTTCCTGATTTG 3606 0.14658691514625766 No Hit TGGCATAGTCACGTTCAGCACTGGATATGTGCTTCCTGATTTGGTCAACC 3159 0.12841599138852686 No Hit CATTGTTTGGCATAGTCACGTTCAGTGCTGGATATTTGTATTTTAATTGG 2881 0.11711505893964734 No Hit GTCACGTTCAATGCTGGGTATGTGTAGTTTAAGTGGGTCAACCAATTTAA 2782 0.11309062616108952 No Hit TTGGCATAGTCACGTTCAATGCTGGGTATGTGTAGTTTAAGTGGGTCAAC 2636 0.1071556040836204 No Hit GCCTCCCTTAGGTCACTAGTTGCCTCGTCCGGCACTCTGGAGTTTATCAC 2462 0.10008235859403393 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0015040809374408022 0.0 0.0 2 0.0015040809374408022 0.0 0.0 3 0.0015040809374408022 0.0 0.0 4 0.0015040809374408022 0.0 0.0 5 0.0015040809374408022 0.0 0.0 6 0.0015040809374408022 0.0 0.0 7 0.0015040809374408022 0.0 0.0 8 0.0015040809374408022 0.0 0.0 9 0.0015040809374408022 0.0 0.0 10 0.0015040809374408022 0.0 0.0 11 0.0015447317735878508 0.0 0.0 12 0.0015447317735878508 0.0 0.0 13 0.0015447317735878508 0.0 0.0 14 0.0015853826097348997 0.0 0.0 15 0.0015853826097348997 0.0 0.0 16 0.0015853826097348997 0.0 0.0 17 0.0015853826097348997 0.0 0.0 18 0.0015853826097348997 0.0 0.0 19 0.0015853826097348997 0.0 0.0 20 0.0016260334458819484 0.0 0.0 21 0.0016260334458819484 0.0 0.0 22 0.0016260334458819484 0.0 4.0650836147048707E-5 23 0.0016260334458819484 0.0 4.0650836147048707E-5 24 0.0016260334458819484 0.0 4.0650836147048707E-5 25 0.0016260334458819484 0.0 4.0650836147048707E-5 26 0.0016260334458819484 0.0 4.0650836147048707E-5 27 0.0016260334458819484 0.0 4.0650836147048707E-5 28 0.0016260334458819484 0.0 4.0650836147048707E-5 29 0.0016260334458819484 0.0 4.0650836147048707E-5 30 0.0017073351181760457 0.0 4.0650836147048707E-5 31 0.0017073351181760457 0.0 4.0650836147048707E-5 32 0.0017073351181760457 0.0 4.0650836147048707E-5 33 0.0017479859543230944 0.0 4.0650836147048707E-5 34 0.001829287626617192 0.0 4.0650836147048707E-5 35 0.0019918909712053866 0.0 4.0650836147048707E-5 36 0.0020731926434994843 0.0 4.0650836147048707E-5 37 0.002235795988087679 0.0 4.0650836147048707E-5 38 0.0024390501688229226 0.0 4.0650836147048707E-5 39 0.0026016535134111172 0.0 4.0650836147048707E-5 40 0.0027236060218522634 0.0 4.0650836147048707E-5 41 0.002926860202587507 0.0 4.0650836147048707E-5 42 0.0030081618748816043 0.0 4.0650836147048707E-5 43 0.0031301143833227505 0.0 4.0650836147048707E-5 44 0.003333368564057994 0.0 4.0650836147048707E-5 45 0.0035772735809402865 0.0 4.0650836147048707E-5 46 0.003617924417087335 0.0 4.0650836147048707E-5 47 0.003983781942410773 0.0 4.0650836147048707E-5 48 0.0042683377954401145 0.0 4.0650836147048707E-5 49 0.0046748461569106016 0.0 4.0650836147048707E-5 50 0.004918751173792894 0.0 4.0650836147048707E-5 51 0.005243957862969283 0.0 4.0650836147048707E-5 52 0.005528513715998625 0.0 4.0650836147048707E-5 53 0.005731767896733868 0.0 4.0650836147048707E-5 54 0.006219577930498452 0.0 4.0650836147048707E-5 55 0.0065041337835277935 0.0 4.0650836147048707E-5 56 0.0069919438172923775 0.0 4.0650836147048707E-5 57 0.007195197998027621 0.0 4.0650836147048707E-5 58 0.007601706359498108 0.0 4.0650836147048707E-5 59 0.008048865557115644 0.0 4.0650836147048707E-5 60 0.008414723082439082 0.0 4.0650836147048707E-5 61 0.00882123144390957 0.0 4.0650836147048707E-5 62 0.009593597330703495 0.0 4.0650836147048707E-5 63 0.009959454856026933 0.0 4.0650836147048707E-5 64 0.010162709036762177 0.0 4.0650836147048707E-5 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCTTAGA 15 0.0022252065 70.00001 6 GGTACAA 15 0.0022252065 70.00001 50 TAGGTAT 15 0.0022252065 70.00001 30 GGGTAAG 15 0.0022252065 70.00001 31 ATGCTAG 15 0.0022252065 70.00001 26 CCTAATC 15 0.0022252065 70.00001 23 AGTTGTC 100 0.0 70.00001 18 TCAGACA 25 2.8419563E-6 70.00001 28 GGAGTAT 25 2.8419563E-6 70.00001 39 TACTCAC 15 0.0022252065 70.00001 14 ATAGTAC 25 2.8419563E-6 70.00001 13 CTAGGTA 15 0.0022252065 70.00001 29 GACTCTA 15 0.0022252065 70.00001 66 AGTACGT 25 2.8419563E-6 70.00001 15 CCCATAG 25 2.8419563E-6 70.00001 5 TAGTCAT 200 0.0 70.00001 14 AAGCTTA 55 0.0 70.00001 54 TAGTACG 25 2.8419563E-6 70.00001 14 GCTTAAC 125 0.0 70.0 42 TTGGTTC 435 0.0 70.0 48 >>END_MODULE