FastQCFastQC Report
Tue 25 Aug 2015
000000000-AHV5L l01n01 kokuya4.34100000033d07.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AHV5L l01n01 kokuya4.34100000033d07.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2459974
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTCCCTTAGGTCACTAGTTGCCTCATCCGGCACACTGGAGTTTAACAA60591724.63103268571131No Hit
TCATTGTTTGGCATAGTCACGTTCAATGCTGGGTATGTGTAGTTTAAGTG41530416.882454855213915No Hit
TCATTGTTTGGCATAGTCACGTTCAGCACTGGATATGTGCTTCCTGATTT27236611.071905638027069No Hit
GCCTCCCTTAGGTCACTAGTTGCCTCGTCAGGCACTCTGGAGTTTATCAC26600510.813325669295692No Hit
GCCTCCCTTAGGTCACTAGTTGCCTCATCCGGCACCCTGGAGTTTAACAA2357889.584979353440321No Hit
TCATTGTTTGGCATAGTCACGTTCAGTGCTGGATATTTGTATTTTAATTG1864267.578372779549703No Hit
ATTGTTTGGCATAGTCACGTTCAATGCTGGGTATGTGTAGTTTAAGTGGG88050.35793061227476386No Hit
CATTGTTTGGCATAGTCACGTTCAATGCTGGGTATGTGTAGTTTAAGTGG72090.29305187778407416No Hit
TGGCATAGTCACGTTCAATGCTGGGTATGTGTAGTTTAAGTGGGTCAACC67570.2746776998456081No Hit
TCATTGTTTGGCATAGTCACTCAAAACGGAACAAGTTCTGCTTGCATAAG58180.23650656470352938No Hit
GCCTCCCTTAGGTCACTAGTTGCCTCGTCAGGCACTCTGGAGTTTAACAC52870.2149209707094465No Hit
CTCCCTTAGGTCACTAGTTGCCTCATCCGGCACACTGGAGTTTAACAATG45940.18674994125954175No Hit
GCCTCCCTTAGGTCACTAGTTGCCTCATCCGGCACACTGGAGTTTAACAC42620.17325386365872159No Hit
TTGTTTGGCATAGTCACGTTCAATGCTGGGTATGTGTAGTTTAAGTGGGT42500.172766053624957No Hit
CCTCCCTTAGGTCACTAGTTGCCTCATCCGGCACACTGGAGTTTAACAAT40610.1650830455931648No Hit
TCATTGTTTGGACTGGAGTCACTCAAAACGGAACAAGTTCTGCTTGCATA40000.16260334458819484No Hit
TGTTTGGCATAGTCACGTTCAATGCTGGGTATGTGTAGTTTAAGTGGGTC38860.15796914926743127No Hit
CATTGTTTGGCATAGTCACGTTCAGCACTGGATATGTGCTTCCTGATTTG36060.14658691514625766No Hit
TGGCATAGTCACGTTCAGCACTGGATATGTGCTTCCTGATTTGGTCAACC31590.12841599138852686No Hit
CATTGTTTGGCATAGTCACGTTCAGTGCTGGATATTTGTATTTTAATTGG28810.11711505893964734No Hit
GTCACGTTCAATGCTGGGTATGTGTAGTTTAAGTGGGTCAACCAATTTAA27820.11309062616108952No Hit
TTGGCATAGTCACGTTCAATGCTGGGTATGTGTAGTTTAAGTGGGTCAAC26360.1071556040836204No Hit
GCCTCCCTTAGGTCACTAGTTGCCTCGTCCGGCACTCTGGAGTTTATCAC24620.10008235859403393No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTTAGA150.002225206570.000016
GGTACAA150.002225206570.0000150
TAGGTAT150.002225206570.0000130
GGGTAAG150.002225206570.0000131
ATGCTAG150.002225206570.0000126
CCTAATC150.002225206570.0000123
AGTTGTC1000.070.0000118
TCAGACA252.8419563E-670.0000128
GGAGTAT252.8419563E-670.0000139
TACTCAC150.002225206570.0000114
ATAGTAC252.8419563E-670.0000113
CTAGGTA150.002225206570.0000129
GACTCTA150.002225206570.0000166
AGTACGT252.8419563E-670.0000115
CCCATAG252.8419563E-670.000015
TAGTCAT2000.070.0000114
AAGCTTA550.070.0000154
TAGTACG252.8419563E-670.0000114
GCTTAAC1250.070.042
TTGGTTC4350.070.048
Produced by FastQC (version 0.11.2)