FastQCFastQC Report
Tue 25 Aug 2015
000000000-AHV5L l01n01 kokuya3.34100000033cf9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AHV5L l01n01 kokuya3.34100000033cf9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1899566
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCATTGTTTGGCATAGTCACGTTCAGCACTGGATATGTGCTTCCTGATTT25027613.175430598357728No Hit
TCATTGTTTGGCATAGTCACGTTCAGTGCTGGATATTTGTATTTTAATTG25008913.165586244436886No Hit
TCATTGTTTGGCATAGTCACGTTCAATGCTGGGTATGTGTAGTTTAAGTG23905212.584558788691732No Hit
GCCTCCCTTAGGTCACTAGTTGCCTCATCCGGCACACTGGAGTTTAACAA23712612.483167207667435No Hit
GCCTCCCTTAGGTCACTAGTTGCCTCATCCGGCACCCTGGAGTTTAACAA22633511.915090078470556No Hit
GCCTCCCTTAGGTCACTAGTTGCCTCGTCAGGCACTCTGGAGTTTATCAC21891411.524421894264268No Hit
ATTGTTTGGCATAGTCACGTTCAATGCTGGGTATGTGTAGTTTAAGTGGG67140.353449156280961No Hit
CTCCCTTAGGTCACTAGTTGCCTCGTCAGGCACTCTGGAGTTTATCACTG57960.30512232794227734No Hit
TGGCATAGTCACGTTCAATGCTGGGTATGTGTAGTTTAAGTGGGTCAACC54360.28617063055455827No Hit
TGGCATAGTCACGTTCAGCACTGGATATGTGCTTCCTGATTTGGTCAACC52440.27606305861444136No Hit
CTCCCTTAGGTCACTAGTTGCCTCATCCGGCACCCTGGAGTTTAACAATG51900.27322030400628355No Hit
CTCCCTTAGGTCACTAGTTGCCTCATCCGGCACACTGGAGTTTAACAATG47180.24837252298682963No Hit
CATTGTTTGGCATAGTCACGTTCAATGCTGGGTATGTGTAGTTTAAGTGG44040.23184243137643018No Hit
GCCTCCCTTAGGTCACTAGTTGCCTCGTCAGGCACTCTGGAGTTTAACAC39550.2082054532456361No Hit
TCATTGTTTGGACTGGAGTCACTCAAAACGGAACAAGTTCTGCTTGCATA37710.19851903013635747No Hit
CATTGTTTGGCATAGTCACGTTCAGTGCTGGATATTTGTATTTTAATTGG36230.19072777676585073No Hit
CCTCCCTTAGGTCACTAGTTGCCTCGTCAGGCACTCTGGAGTTTATCACT36200.1905698459542864No Hit
TCATTGTTTGGCATAGTCACTCAAAACGGAACAAGTTCTGCTTGCATAAG36060.18983283550031954No Hit
GCCTCCCTTAGGTCACTAGTTGCCTCGTCAGGCACTCTGGAGTTTAACAA34180.1799358379756218No Hit
TTGTTTGGCATAGTCACGTTCAATGCTGGGTATGTGTAGTTTAAGTGGGT33510.17640871651735188No Hit
CATTGTTTGGCATAGTCACGTTCAGCACTGGATATGTGCTTCCTGATTTG31780.16730137305047574No Hit
TGTTTGGCATAGTCACGTTCAATGCTGGGTATGTGTAGTTTAAGTGGGTC30420.1601418429262263No Hit
TTGGCATAGTCACGTTCAATGCTGGGTATGTGTAGTTTAAGTGGGTCAAC29670.15619357263711817No Hit
CCTCCCTTAGGTCACTAGTTGCCTCATCCGGCACACTGGAGTTTAACAAT28820.15171886630946227No Hit
CCTCCCTTAGGTCACTAGTTGCCTCATCCGGCACCCTGGAGTTTAACAAT27990.14734944718951593No Hit
GCCTCCCTTAGGTCACTAGTTGAACGAAGCAAAGCCTACAGCAACTGTTA27890.14682301115096819No Hit
TTGGCATAGTCACGTTCAGCACTGGATATGTGCTTCCTGATTTGGTCAAC26980.14203244320018363No Hit
TTGGCATAGTCACGTTCAGTGCTGGATATTTGTATTTTAATTGGTGCAAC25970.13671543921085133No Hit
ATTGTTTGGCATAGTCACGTTCAGTGCTGGATATTTGTATTTTAATTGGT25350.13345153577185526No Hit
TCCCTTAGGTCACTAGTTGCCTCATCCGGCACACTGGAGTTTAACAATGA25080.13203015846777633No Hit
GGTCACTAGTTGCCTCGTCAGGCACTCTGGAGTTTATCACTGAGGGTTTC25080.13203015846777633No Hit
GTCACGTTCAATGCTGGGTATGTGTAGTTTAAGTGGGTCAACCAATTTAA24740.13024027593671397No Hit
TCCCTTAGGTCACTAGTTGCCTCATCCGGCACCCTGGAGTTTAACAATGA21670.11407868955329796No Hit
GCCTCCCTTAGGTCACTAGTTGCCTCGTCAGGCACCCTGGAGTTTATCAC21620.1138154715340241No Hit
CCCTTAGGTCACTAGTTGCCTCGTCAGGCACTCTGGAGTTTATCACTGAG21180.11149915296441398No Hit
CCTTAGGTCACTAGTTGCCTCGTCAGGCACTCTGGAGTTTATCACTGAGG20890.1099724884526255No Hit
TGTTTGGCATAGTCACGTTCAGTGCTGGATATTTGTATTTTAATTGGTGC20730.10913019079094909No Hit
TCCCTTAGGTCACTAGTTGCCTCGTCAGGCACTCTGGAGTTTATCACTGA20650.10870904196011089No Hit
CCCTTAGGTCACTAGTTGCCTCATCCGGCACCCTGGAGTTTAACAATGAA19760.1040237612170359No Hit
GTTTGGCATAGTCACGTTCAATGCTGGGTATGTGTAGTTTAAGTGGGTCA19520.10276031472452127No Hit
GCCTCCCTTAGGTCACTAGTTGCCTCATCCGGCACACTGGAGTTTATCAC19100.10054928336262073No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACTAT550.070.0000167
ACTCTTA252.841707E-670.0000169
GGACTCT252.841707E-670.0000165
GGGTAAA353.7161954E-970.0000150
TTACTAG353.7161954E-970.0000113
TAAGTGT252.841707E-670.0000145
ATGTATA252.841707E-670.0000135
GTGTGTC252.841707E-670.0000148
CATAGTA550.070.0000112
TGTCTCG252.841707E-670.0000121
TCTCGTA700.070.0000141
ATAGTAC252.841707E-670.0000113
GATTACG252.841707E-670.0000167
AGTGTGT252.841707E-670.0000147
TAGTACG252.841707E-670.0000114
CTAGATA207.929946E-570.029
ATCCTAA401.3460522E-1070.041
AGGTATG150.002225128770.031
GAGTGTA150.002225128770.040
TTCTAAA207.929946E-570.068
Produced by FastQC (version 0.11.2)