##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename 000000000-AHV5L l01n01 kokuya3.34100000033cf9.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1899566 Sequences flagged as poor quality 0 Sequence length 76 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 33.61636921275702 34.0 34.0 34.0 34.0 34.0 2 33.79390555526894 34.0 34.0 34.0 34.0 34.0 3 33.79676252365014 34.0 34.0 34.0 34.0 34.0 4 33.84784050672627 34.0 34.0 34.0 34.0 34.0 5 33.89352778476768 34.0 34.0 34.0 34.0 34.0 6 37.72785099333216 38.0 38.0 38.0 38.0 38.0 7 37.72830478119739 38.0 38.0 38.0 38.0 38.0 8 37.7406397040166 38.0 38.0 38.0 38.0 38.0 9 37.711972103101445 38.0 38.0 38.0 38.0 38.0 10-11 37.510734557262026 38.0 38.0 38.0 37.0 38.0 12-13 37.545264549902456 38.0 38.0 38.0 37.0 38.0 14-15 37.539047603505225 38.0 38.0 38.0 37.0 38.0 16-17 37.698931755990586 38.0 38.0 38.0 37.5 38.0 18-19 37.664659190573005 38.0 38.0 38.0 37.5 38.0 20-21 37.56969539357938 38.0 38.0 38.0 37.0 38.0 22-23 37.57981796894659 38.0 38.0 38.0 37.0 38.0 24-25 37.659086601886955 38.0 38.0 38.0 37.5 38.0 26-27 37.1548890641336 38.0 38.0 38.0 36.5 38.0 28-29 37.42037628595163 38.0 38.0 38.0 37.0 38.0 30-31 37.38235128445129 38.0 38.0 38.0 37.0 38.0 32-33 37.40435157293824 38.0 38.0 38.0 37.0 38.0 34-35 37.48034814268101 38.0 38.0 38.0 37.0 38.0 36-37 37.11565483905271 38.0 38.0 38.0 36.5 38.0 38-39 37.56236950966695 38.0 38.0 38.0 37.0 38.0 40-41 37.12144589869476 38.0 38.0 38.0 36.5 38.0 42-43 37.16648829258894 38.0 38.0 38.0 37.0 38.0 44-45 37.24347798391844 38.0 38.0 38.0 37.0 38.0 46-47 37.02572982460204 38.0 38.0 38.0 36.5 38.0 48-49 37.32533878791261 38.0 38.0 38.0 37.0 38.0 50-51 37.08973918252906 38.0 38.0 38.0 36.5 38.0 52-53 37.070894614875186 38.0 38.0 38.0 36.5 38.0 54-55 37.03784548681119 38.0 38.0 38.0 37.0 38.0 56-57 37.1638411089691 38.0 38.0 38.0 37.0 38.0 58-59 37.301941601397374 38.0 38.0 38.0 36.5 38.0 60-61 37.22012291228628 38.0 38.0 38.0 37.0 38.0 62-63 37.02843254722394 38.0 38.0 38.0 36.5 38.0 64-65 37.23512160146055 38.0 38.0 38.0 37.0 38.0 66-67 37.37631332630717 38.0 38.0 38.0 37.0 38.0 68-69 37.444311753316285 38.0 38.0 38.0 37.0 38.0 70-71 37.41052903663258 38.0 38.0 38.0 37.0 38.0 72-73 36.99752548740081 38.0 38.0 38.0 36.5 38.0 74-75 37.07975479662196 38.0 38.0 38.0 36.0 38.0 76 36.25426386869422 38.0 37.0 38.0 34.0 38.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.017881548362893795 1101 2 -0.00349608810041957 1101 3 0.07411974051773029 1101 4 0.01647285677751853 1101 5 0.019063188387278274 1101 6 -0.0447767854014387 1101 7 -0.08691037424613057 1101 8 -0.11826846368774824 1101 9 -0.40635234884811666 1101 10-11 -0.5491800109175102 1101 12-13 -0.7911683732746511 1101 14-15 -0.312857322525808 1101 16-17 -0.0590537925992507 1101 18-19 0.005712815689093986 1101 20-21 0.1515405871815716 1101 22-23 0.09368443687073835 1101 24-25 0.0870408251889856 1101 26-27 0.11705085479019317 1101 28-29 0.10228756181550835 1101 30-31 0.14626101960762838 1101 32-33 0.15597496513915843 1101 34-35 0.14558893366818637 1101 36-37 0.14460871219205984 1101 38-39 0.07262567855146074 1101 40-41 0.1709966627902375 1101 42-43 0.14150427894464457 1101 44-45 0.16842696415336889 1101 46-47 0.2000017574966293 1101 48-49 0.14452663240670205 1101 50-51 0.22055620660200503 1101 52-53 0.2292215632609853 1101 54-55 0.27895742700923876 1101 56-57 0.18117159578802244 1101 58-59 0.12861812371299663 1101 60-61 0.1751034274558947 1101 62-63 0.1268053980485604 1101 64-65 0.14993192786459986 1101 66-67 0.2093667461763431 1101 68-69 0.058575197856647776 1101 70-71 0.13682100920855333 1101 72-73 0.21088652252651485 1101 74-75 0.15507262816907286 1101 76 0.26827249901792527 1102 1 0.10107694957261515 1102 2 0.02029362070508256 1102 3 0.02972339440924543 1102 4 0.025293911666018687 1102 5 0.04596214586859304 1102 6 -0.013840300021406904 1102 7 0.030714706564168637 1102 8 -0.028932565815424027 1102 9 -0.2237604231351895 1102 10-11 -0.18057776989546426 1102 12-13 0.01934729358361409 1102 14-15 0.12297681233040691 1102 16-17 0.053780073954648344 1102 18-19 0.08117881653819836 1102 20-21 0.16967265575901536 1102 22-23 0.129038886289905 1102 24-25 0.09546408491925007 1102 26-27 0.0872750840017531 1102 28-29 0.11145274722109377 1102 30-31 0.1709054772596872 1102 32-33 0.1383311215492924 1102 34-35 0.1427362057371795 1102 36-37 0.13074002674891005 1102 38-39 0.06285954943362526 1102 40-41 0.1283942907504283 1102 42-43 0.11202818621850241 1102 44-45 0.14017101610993876 1102 46-47 0.22326131646644143 1102 48-49 0.09005234242813032 1102 50-51 0.18075649081362144 1102 52-53 0.22301965202267837 1102 54-55 0.3140463106117437 1102 56-57 0.25819600194041215 1102 58-59 0.16653226580192637 1102 60-61 0.16859594736946804 1102 62-63 0.19586767602129385 1102 64-65 0.12942263475304827 1102 66-67 0.18957696450891603 1102 68-69 0.09890911600714247 1102 70-71 0.1302486678100223 1102 72-73 0.25945247965012186 1102 74-75 0.2381983844143818 1102 76 0.47287837494727825 1103 1 0.017308979931726753 1103 2 0.02749184840816099 1103 3 0.01664269174914068 1103 4 -3.979660831845422E-4 1103 5 -0.006704583794409302 1103 6 0.07026915585669968 1103 7 0.016244883473760297 1103 8 0.06027741659426056 1103 9 0.052475182137960985 1103 10-11 0.19108889805161766 1103 12-13 0.18153961986160994 1103 14-15 0.13094271185023132 1103 16-17 0.06879464889966869 1103 18-19 0.029293459114661857 1103 20-21 0.1601218606095287 1103 22-23 0.09044300292121932 1103 24-25 0.05383054894770822 1103 26-27 0.08935402711785656 1103 28-29 -0.027690026292482628 1103 30-31 0.056753747254816744 1103 32-33 0.10108050445408168 1103 34-35 0.10559845707771132 1103 36-37 -0.0653740340077249 1103 38-39 0.03362912138376828 1103 40-41 0.05350387492595843 1103 42-43 2.3990717340893752E-4 1103 44-45 -0.019234426517435566 1103 46-47 0.05107952542938676 1103 48-49 -0.09903557817803943 1103 50-51 0.04725381001221507 1103 52-53 -0.07145748991036527 1103 54-55 0.1670299553800163 1103 56-57 0.17676280200053185 1103 58-59 0.12908126401933373 1103 60-61 0.05452392309488374 1103 62-63 0.08653580594432242 1103 64-65 0.14113681627827646 1103 66-67 0.14622351332121752 1103 68-69 0.0338250018137316 1103 70-71 0.14768783345172665 1103 72-73 0.2010991782707876 1103 74-75 0.15665045943942602 1103 76 0.3792399549670904 2116 1 -0.036488665026134015 2116 2 0.028567974588348477 2116 3 -0.040610380867590834 2116 4 0.019990968291971 2116 5 -0.008781917590511057 2116 6 0.026430965685356966 2116 7 0.06687492566513953 2116 8 0.042283455438258954 2116 9 0.051115435678688925 2116 10-11 0.2236917881244267 2116 12-13 0.16922054883141868 2116 14-15 0.1086662246339074 2116 16-17 0.0426181917272217 2116 18-19 0.05224994697314145 2116 20-21 0.13057062790609564 2116 22-23 0.09894052640397888 2116 24-25 0.07795126874692215 2116 26-27 0.12484918149213797 2116 28-29 0.08661977624104367 2116 30-31 0.15906950810762766 2116 32-33 0.03395608281138607 2116 34-35 0.1435061038932517 2116 36-37 0.14072598074664455 2116 38-39 0.08802369401794863 2116 40-41 0.10113252398879524 2116 42-43 0.11154073553318966 2116 44-45 0.06285873701067857 2116 46-47 0.2187401371980755 2116 48-49 0.11847066057621447 2116 50-51 0.19107948827367238 2116 52-53 0.15931768024268678 2116 54-55 0.2359561175287581 2116 56-57 0.1917511654146935 2116 58-59 0.18633325362960562 2116 60-61 0.13533480697849853 2116 62-63 0.17541324044727702 2116 64-65 0.19198301381712923 2116 66-67 0.17337773420509706 2116 68-69 -0.05098376855092823 2116 70-71 0.1073146905848219 2116 72-73 0.28701089515200096 2116 74-75 0.14380759552186362 2116 76 0.43778116820443813 1117 1 -0.07305876320808835 1117 2 -0.04412238398354873 1117 3 -0.06858297210722242 1117 4 -0.006759548206353827 1117 5 0.018534530648942393 1117 6 0.03851801387799725 1117 7 0.07541016288882219 1117 8 0.03822373869405027 1117 9 0.07615597192096857 1117 10-11 0.1514992091454488 1117 12-13 0.19296993380240224 1117 14-15 0.1645275404745803 1117 16-17 0.05896199704569227 1117 18-19 -0.0031566360537809146 1117 20-21 0.1513706257211922 1117 22-23 0.0476251731367725 1117 24-25 -0.10582438127205052 1117 26-27 0.08024969301141027 1117 28-29 0.048567641948537243 1117 30-31 -0.025068142424800044 1117 32-33 0.07953949167091423 1117 34-35 0.09274764863605611 1117 36-37 0.1559334687433349 1117 38-39 0.019968038671180466 1117 40-41 0.12155310509719897 1117 42-43 -0.2671314236419917 1117 44-45 -0.09755379357270044 1117 46-47 -0.2214568989818062 1117 48-49 -0.0396091715560587 1117 50-51 -0.2131677099041127 1117 52-53 0.009440076794909658 1117 54-55 -0.09180916008620699 1117 56-57 0.11898046966193476 1117 58-59 0.02284106108424311 1117 60-61 0.09697693340955738 1117 62-63 0.04225520351486267 1117 64-65 0.11596120428935563 1117 66-67 0.14584271169010066 1117 68-69 0.18704065529660596 1117 70-71 0.0948658099481392 1117 72-73 0.08042908673348137 1117 74-75 0.18649993731287395 1117 76 0.43753750956854986 2117 1 -0.013829100195941635 2117 2 -5.973466063551314E-4 2117 3 -0.006116928037549485 2117 4 0.017461042426695883 2117 5 0.020691405475261604 2117 6 -0.028902861102558575 2117 7 0.033076920509806484 2117 8 -0.0663791786343424 2117 9 -0.22815367011719445 2117 10-11 -0.3501536027052694 2117 12-13 -0.8222232519166823 2117 14-15 -1.1375669237604669 2117 16-17 -0.07452614724725493 2117 18-19 0.05080260132148595 2117 20-21 0.15887710254901322 2117 22-23 0.1403399626026669 2117 24-25 0.06982054563546569 2117 26-27 0.1758006854823151 2117 28-29 0.13252070113632186 2117 30-31 0.13793679551459803 2117 32-33 0.1516764529499426 2117 34-35 0.19349018378026273 2117 36-37 0.2174316037606232 2117 38-39 0.15031939060026644 2117 40-41 0.24620560300881777 2117 42-43 0.23188463503486645 2117 44-45 0.25427845396723825 2117 46-47 0.052483095477100505 2117 48-49 0.23902570049803984 2117 50-51 0.2425390449840492 2117 52-53 0.2739550869822054 2117 54-55 0.25159679838581184 2117 56-57 0.06819316990290503 2117 58-59 0.18432184352611358 2117 60-61 0.2476282417895561 2117 62-63 0.27186456518738567 2117 64-65 0.17909580452835172 2117 66-67 0.20886693026884018 2117 68-69 0.2441347150573705 2117 70-71 0.21758944755092102 2117 72-73 0.14392216016380388 2117 74-75 0.15242951771167412 2117 76 0.30993081952200896 1116 1 0.06356654323941768 1116 2 0.0077472621486762705 1116 3 0.03750717112376378 1116 4 -0.03353053216225277 1116 5 -0.02053223230367962 1116 6 -0.009235154363153697 1116 7 0.03999790768828149 1116 8 -0.1083357817208821 1116 9 -0.21193496769389952 1116 10-11 -0.2476755971156095 1116 12-13 -0.034746165468874324 1116 14-15 0.1265059891795275 1116 16-17 0.041195845385182395 1116 18-19 0.03244750210532743 1116 20-21 0.17107673982343385 1116 22-23 0.11731732521234761 1116 24-25 0.056481394704491095 1116 26-27 0.07295215083427564 1116 28-29 0.1582524797781204 1116 30-31 0.059714092140616515 1116 32-33 0.07224131677354251 1116 34-35 0.11661162053315621 1116 36-37 0.07685929874574526 1116 38-39 0.042598712273580475 1116 40-41 0.23731015908426656 1116 42-43 0.1978059623629136 1116 44-45 0.2197842595724282 1116 46-47 0.08392898443906205 1116 48-49 0.1495001101967901 1116 50-51 0.15757142544570968 1116 52-53 0.2015481572229305 1116 54-55 0.09264178216078989 1116 56-57 0.1727136034493597 1116 58-59 0.16779764803940367 1116 60-61 0.15082910296508345 1116 62-63 0.021845968498944046 1116 64-65 0.17814355763746192 1116 66-67 0.10795268184257623 1116 68-69 0.17743954744565116 1116 70-71 0.10041615845045015 1116 72-73 0.09248101534914355 1116 74-75 0.2646728184171465 1116 76 0.1717999011835758 2118 1 -0.13780592340940245 2118 2 0.0330527067913593 2118 3 0.04279453786104881 2118 4 -0.028426642922362078 2118 5 0.010745267458538876 2118 6 0.03863281712862232 2118 7 0.04625671406679999 2118 8 -0.0097734274389083 2118 9 0.06854211877339367 2118 10-11 0.1645770809645768 2118 12-13 0.18657262880662273 2118 14-15 0.13656295881754232 2118 16-17 0.0730096816039989 2118 18-19 0.06361616881274301 2118 20-21 0.16474485060152944 2118 22-23 0.06531542580798799 2118 24-25 0.05213379837631038 2118 26-27 0.012445053345018664 2118 28-29 0.05854282896169849 2118 30-31 0.06804407879332075 2118 32-33 0.06359923931189115 2118 34-35 0.12744272695660186 2118 36-37 0.10305314759393269 2118 38-39 0.054585360475343236 2118 40-41 0.09085815831338095 2118 42-43 0.159347667222832 2118 44-45 0.21361552623677227 2118 46-47 0.08110094907745946 2118 48-49 0.09410348148545467 2118 50-51 0.1526582595235908 2118 52-53 0.1934584694578234 2118 54-55 0.2079929104516154 2118 56-57 0.11552492511864898 2118 58-59 0.06583052857281757 2118 60-61 0.0885541918762982 2118 62-63 0.09644999690856082 2118 64-65 0.08906754381809634 2118 66-67 0.1762264450934765 2118 68-69 0.16114425961084322 2118 70-71 0.1175494628893432 2118 72-73 0.15192374763307015 2118 74-75 0.23491146492455783 2118 76 0.3773786498420577 1119 1 -0.021838036827226404 1119 2 0.007296889039203336 1119 3 0.004700618265459866 1119 4 0.005570140273384538 1119 5 0.0015494742449675414 1119 6 0.0462523042994718 1119 7 0.10506360498518319 1119 8 0.04747682395314001 1119 9 0.1146340957587455 1119 10-11 0.21756417539583595 1119 12-13 0.22493176016644867 1119 14-15 0.1545259501134879 1119 16-17 0.03745484828977652 1119 18-19 0.07265587694038089 1119 20-21 0.15582968344881465 1119 22-23 0.09330862667214035 1119 24-25 0.062166992675678046 1119 26-27 0.09135803387549402 1119 28-29 0.021719157802863265 1119 30-31 0.1273771205770231 1119 32-33 0.10136496113805293 1119 34-35 0.19511314508964261 1119 36-37 0.0696193542058623 1119 38-39 -0.017811154601602652 1119 40-41 0.10577357552932654 1119 42-43 0.12599713965727943 1119 44-45 0.18799032113872016 1119 46-47 0.02537385098111855 1119 48-49 0.0611985579301475 1119 50-51 0.12046341578162867 1119 52-53 0.15183338800124346 1119 54-55 0.1048596132566999 1119 56-57 0.12418268987227776 1119 58-59 0.060650404952617976 1119 60-61 0.1497796963306186 1119 62-63 0.11466239724818195 1119 64-65 0.10830436113954534 1119 66-67 0.17735567520374218 1119 68-69 0.07075999632386498 1119 70-71 0.08955313419046718 1119 72-73 0.11493358571591017 1119 74-75 0.15886666795291404 1119 76 -0.04332779595886649 2119 1 -0.011172090576287985 2119 2 -0.010606201526414338 2119 3 0.01375945936357681 2119 4 -0.014694553291306534 2119 5 0.034041388833038866 2119 6 0.10986625123859994 2119 7 0.07941643742842075 2119 8 0.07708657542512753 2119 9 0.137482081578419 2119 10-11 0.20140314625801636 2119 12-13 0.20822863468862352 2119 14-15 0.1840990170022394 2119 16-17 0.035939626965948435 2119 18-19 0.07777466401381616 2119 20-21 0.1725626189528171 2119 22-23 0.11218139480359213 2119 24-25 -0.04919511299748791 2119 26-27 0.12127290211882524 2119 28-29 0.09196031969791107 2119 30-31 0.08541330337843078 2119 32-33 0.07007177462308078 2119 34-35 0.1384453195497528 2119 36-37 0.07586159396656456 2119 38-39 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-0.02082281863793156 2104 48-49 0.0017323753296309974 2104 50-51 -0.06988124035123633 2104 52-53 -0.10696181627080392 2104 54-55 -0.11701639335363012 2104 56-57 -0.0796953756044374 2104 58-59 0.11961353888425919 2104 60-61 -0.005884395103457507 2104 62-63 -0.04443357404131376 2104 64-65 -0.15409949210945229 2104 66-67 -0.14633172102713843 2104 68-69 0.014545303375875562 2104 70-71 -0.14777640038712292 2104 72-73 -0.24743214961667803 2104 74-75 -0.05028628708685545 2104 76 -0.30958727605044345 2111 1 0.019459517566140505 2111 2 -0.04625846961966573 2111 3 -0.060417793795657815 2111 4 -0.010211970020037597 2111 5 -0.005183406488875164 2111 6 -0.2033887667185894 2111 7 -0.06234347469921886 2111 8 -0.08741872288270258 2111 9 -0.11186552944092654 2111 10-11 -0.39314160851650115 2111 12-13 -0.46852733336486807 2111 14-15 -0.3437536552676974 2111 16-17 -0.06547803820112108 2111 18-19 -0.0995037931249243 2111 20-21 0.0929938862124402 2111 22-23 -0.10154805212772544 2111 24-25 -0.009598259686583788 2111 26-27 -0.04296764296342559 2111 28-29 0.016337076373595494 2111 30-31 -0.09773479704813326 2111 32-33 -0.10670280812374955 2111 34-35 -0.06909054518531121 2111 36-37 -0.01218973855652905 2111 38-39 -0.06612181261374417 2111 40-41 -0.08536849883373776 2111 42-43 -0.008648803173535669 2111 44-45 0.04224068696829164 2111 46-47 0.026585357055409986 2111 48-49 -0.0333604887954877 2111 50-51 -0.08391425597688595 2111 52-53 -0.2534751923434513 2111 54-55 -0.0323038600165475 2111 56-57 -0.16453461762211674 2111 58-59 -0.25525502864660865 2111 60-61 -0.02470718605226807 2111 62-63 -0.05512717829488878 2111 64-65 -0.058076483310628646 2111 66-67 -0.08510323181462809 2111 68-69 -0.17240633497439006 2111 70-71 -0.09063552385086382 2111 72-73 0.004441483336464103 2111 74-75 -0.015129425133210361 2111 76 -0.2502810616506963 2110 1 0.03129911715299727 2110 2 -0.036389811953533524 2110 3 0.005767837625057837 2110 4 -0.008527366836652561 2110 5 0.02763304161971547 2110 6 -0.13907551859523437 2110 7 -0.019682691578914557 2110 8 -0.02226363426063216 2110 9 -0.07606304322760593 2110 10-11 -0.4155325715552891 2110 12-13 -0.4198651946312211 2110 14-15 -0.14705041240080874 2110 16-17 -0.05533964114474088 2110 18-19 -0.11095595933971936 2110 20-21 -0.08050842616883358 2110 22-23 -0.06361543164413774 2110 24-25 -0.1110115672909302 2110 26-27 -0.023000415146611886 2110 28-29 -0.042153893120492114 2110 30-31 -0.09925663441141808 2110 32-33 -0.0920408584382102 2110 34-35 -0.01771026336528081 2110 36-37 0.017903494442968793 2110 38-39 0.047757547020324864 2110 40-41 -0.13632463275357765 2110 42-43 0.002452644197305176 2110 44-45 -0.1605213752239294 2110 46-47 0.029542634485835606 2110 48-49 -0.07698224731186798 2110 50-51 0.016267414655182222 2110 52-53 -0.07582972903723117 2110 54-55 -0.15369661440154658 2110 56-57 -0.11843021925623276 2110 58-59 -0.1199860846417451 2110 60-61 -0.19068175216377625 2110 62-63 -0.35261802559934097 2110 64-65 -0.2733278827278056 2110 66-67 -0.31024916774114786 2110 68-69 -0.34008925055748307 2110 70-71 -0.21450555787953363 2110 72-73 -0.3389690945974664 2110 74-75 -0.1691346516220804 2110 76 -0.27029192039025673 2109 1 0.01707715996668213 2109 2 0.025676780322136494 2109 3 0.02813394309001893 2109 4 -0.01075894242061537 2109 5 -0.004576839675451083 2109 6 -0.1661276860502241 2109 7 -0.1028750768033575 2109 8 -0.04975213943345835 2109 9 -0.20794698587622662 2109 10-11 -0.4317967727712002 2109 12-13 -0.41069724493581106 2109 14-15 -0.09878435942899699 2109 16-17 -0.07778547327523455 2109 18-19 -0.06555123779396865 2109 20-21 0.0486936086037133 2109 22-23 -0.030699499017948995 2109 24-25 -0.04490146831266628 2109 26-27 -0.07671664157404479 2109 28-29 -0.20943944381122748 2109 30-31 -0.08422283770732264 2109 32-33 -0.06911944209785048 2109 34-35 -0.11877823057896109 2109 36-37 -0.10586159039730347 2109 38-39 -0.024546982317843913 2109 40-41 -0.318542741162112 2109 42-43 -0.2501506935896103 2109 44-45 -0.35988946814975264 2109 46-47 -0.13328639607492931 2109 48-49 -0.2370540768738394 2109 50-51 -0.13478886342649332 2109 52-53 -0.46550371482768327 2109 54-55 -0.24293103177078734 2109 56-57 -0.04398737294332733 2109 58-59 0.030991739008428 2109 60-61 -0.14976084595070915 2109 62-63 0.013252808566733165 2109 64-65 -0.02109673122185285 2109 66-67 -0.12011494936471934 2109 68-69 0.010825277522343413 2109 70-71 -0.07101826263981081 2109 72-73 -0.27112875734597 2109 74-75 -0.24292272149039462 2109 76 -0.41365565471941323 2108 1 -0.021649506048575518 2108 2 0.009562311352510733 2108 3 -0.01294697278697754 2108 4 0.030756623183535226 2108 5 -0.03312563786047207 2108 6 0.01240692586487313 2108 7 -0.034089713794870136 2108 8 0.07105534351352105 2108 9 0.01586430765940605 2108 10-11 0.09313491218363623 2108 12-13 0.1279284321125047 2108 14-15 0.06611922907647738 2108 16-17 -0.009606815270920777 2108 18-19 0.009459348888306351 2108 20-21 0.08707223093220762 2108 22-23 -0.08209008123187544 2108 24-25 -0.0014092335891646712 2108 26-27 0.03364093186198147 2108 28-29 0.018467725464553553 2108 30-31 -0.08239290914304576 2108 32-33 -0.11517538528120497 2108 34-35 -0.09005654807424435 2108 36-37 -0.0642381528439202 2108 38-39 0.03097178902083897 2108 40-41 -0.0664416385199118 2108 42-43 0.08809785386976188 2108 44-45 0.032242110872701346 2108 46-47 0.1305963478296306 2108 48-49 -9.542113946920949E-4 2108 50-51 0.06602335933102665 2108 52-53 -0.08614901388960305 2108 54-55 -0.04392824287120334 2108 56-57 -0.05777579878253647 2108 58-59 -0.061173128969805646 2108 60-61 -0.1155808042548756 2108 62-63 -0.11393529535712332 2108 64-65 -0.09287524680049586 2108 66-67 -0.1407839474793633 2108 68-69 -0.29682135718518055 2108 70-71 -0.07174947828707445 2108 72-73 0.0024236705036102535 2108 74-75 -0.026237628225409537 2108 76 0.061531714711179575 >>END_MODULE >>Per sequence quality scores pass #Quality Count 14 3.0 15 16.0 16 68.0 17 33.0 18 49.0 19 78.0 20 111.0 21 151.0 22 252.0 23 362.0 24 496.0 25 670.0 26 986.0 27 1379.0 28 2156.0 29 3421.0 30 5716.0 31 10731.0 32 19960.0 33 31023.0 34 53488.0 35 89903.0 36 181793.0 37 1496721.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.81921975861855 2.19007920756636 48.836523711205615 5.15417732260948 2 3.4553682262158834 2.3774377936855053 4.933547978854117 89.2336460012445 3 3.5597078490560476 45.98997876357021 5.154335253421045 45.2959781339527 4 3.49832540696138 2.0013518877469907 89.47643830222272 5.023884403068911 5 3.480953017689304 2.5192596624702697 48.55024779344335 45.44953952639708 6 47.5322257820997 2.4351351835103388 5.862233794456207 44.17040523993375 7 3.538966269137266 3.009529545169791 50.069173695465174 43.382330490227766 8 4.655063314462357 3.527805825120054 89.28686868474168 2.530262175675918 9 5.688773119754723 2.9466730821671896 88.27742758082636 3.0871262172517304 10-11 68.86291394981802 23.610077249224297 3.479636927592934 4.047371873364758 12-13 23.435379449832226 25.796866231549732 24.0841855455404 26.68356877307764 14-15 3.724166467498365 45.53850721691165 26.378709663154638 24.358616652435348 16-17 25.643489091718845 3.643305891977431 46.20139547665098 24.51180953965274 18-19 24.450427097558077 44.8751188429357 4.760192591360343 25.91426146814588 20-21 26.258708568167677 2.477329031999941 45.002753260481605 26.26120913935078 22-23 23.645374785608922 2.813326833603044 47.70976633609993 25.831532044688103 24-25 4.507450649253567 25.685182825971825 23.574411207612687 46.232955317161924 26-27 25.848430641525482 23.854948972554784 18.666290089420425 31.630330296499302 28-29 19.531935189406422 10.29153501378736 24.010695074559134 46.165834722247084 30-31 48.3097454892328 9.766309778128267 25.14708622917024 16.776858503468688 32-33 52.19394851244969 19.200096232507846 4.391897938792335 24.21405731625013 34-35 3.9239489441272375 44.977958123066 26.93030934434497 24.167783588461784 36-37 18.982388608766424 9.45805515575663 41.70244676941996 29.857109466056986 38-39 47.036691539014704 3.809317496733464 46.34284884020876 2.8111421240430707 40-41 24.495384735250052 37.86346460191433 27.638681677814823 10.002468985020789 42-43 30.52881552944199 3.1427705065262277 55.77131829059901 10.557095673432773 44-45 3.8452204345624215 5.106192677695852 80.7715025432125 10.277084344529223 46-47 11.239119883173315 74.13035398612105 10.558648659746488 4.071877470959156 48-49 11.226643349059733 24.819669334995467 40.56005424396941 23.39363307197539 50-51 40.90744938580708 17.68809296439292 30.819960980560822 10.584496669239183 52-53 45.61797273693043 25.256348028970827 24.98699702984787 4.138682204250866 54-55 17.130176050740012 52.93598643058467 4.21138302117431 25.72245449750101 56-57 22.533752446611487 25.89494126553118 11.399077473486049 40.17222881437128 58-59 2.724622361107748 26.37447185304433 44.94708264940518 25.953823136442743 60-61 10.604685491317491 38.746060942341565 27.298472387903345 23.3507811784376 62-63 3.748119307252288 17.638739585779067 53.69250133977972 24.920639767188927 64-65 32.077090240612854 40.49188077697748 23.853001159212155 3.578027823197509 66-67 23.668616936710805 24.797164194347552 25.444943739780562 26.08927512916108 68-69 4.502870655718201 26.562699058627075 45.27671057494185 23.65771971071287 70-71 44.40885444359396 5.680823935572652 24.97459946114007 24.93572215969332 72-73 37.7014012674474 54.68020063530301 3.776783749551213 3.8416143476983686 74-75 17.653321864046838 34.12492643056361 23.29750585133657 24.92424585405298 76 16.239709491536487 75.16085253157827 3.5866613742296924 5.012776602655554 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.5 11 0.5 12 0.0 13 0.5 14 0.5 15 0.5 16 5.0 17 9.0 18 7.0 19 6.5 20 10.5 21 13.0 22 16.0 23 19.0 24 26.0 25 33.0 26 39.0 27 101.0 28 344.5 29 532.0 30 1641.0 31 2834.0 32 12342.0 33 21766.0 34 136953.5 35 149685.0 36 156892.5 37 266556.0 38 150432.0 39 23571.5 40 15903.0 41 134767.0 42 250563.0 43 139595.5 44 23850.0 45 14651.0 46 10230.0 47 16776.0 48 128702.5 49 149619.0 50 65155.0 51 146709.5 52 138971.5 53 137751.5 54 225824.0 55 128253.0 56 18639.5 57 5086.5 58 3576.0 59 3434.5 60 3440.5 61 3852.0 62 4116.0 63 4465.0 64 4966.5 65 5417.5 66 5728.0 67 5740.0 68 5759.5 69 5636.5 70 5194.5 71 4895.0 72 4534.5 73 3776.0 74 3018.5 75 2659.0 76 2232.5 77 1509.5 78 1011.0 79 809.0 80 633.5 81 356.5 82 199.0 83 143.0 84 105.0 85 54.5 86 26.5 87 11.0 88 7.0 89 2.5 90 1.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 1899566.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 6.192469379258906 #Duplication Level Percentage of deduplicated Percentage of total 1 88.48655997210064 5.4795031310318985 2 5.135156566816106 0.6359859959541805 3 1.9336673218538467 0.35922527040760566 4 1.0400719371068905 0.2576245449104365 5 0.5356855838809025 0.16586082875464583 6 0.36471719353269055 0.13551000318242581 7 0.2779549511317795 0.1204859266587865 8 0.22467873727815996 0.11130529606124494 9 0.1507379275719085 0.0840096000703791 >10 1.1860994089774919 1.5496963885424777 >50 0.2588494599455463 1.1540942145240323 >100 0.2669953882258531 3.6394346982935275 >500 0.08176228804614445 3.317124656530673 >1k 0.04769466802680058 6.507091549364616 >5k 0.004258452502392909 1.4967803124597203 >10k+ 0.0051101430028714905 74.98626758325337 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source TCATTGTTTGGCATAGTCACGTTCAGCACTGGATATGTGCTTCCTGATTT 250276 13.175430598357728 No Hit TCATTGTTTGGCATAGTCACGTTCAGTGCTGGATATTTGTATTTTAATTG 250089 13.165586244436886 No Hit TCATTGTTTGGCATAGTCACGTTCAATGCTGGGTATGTGTAGTTTAAGTG 239052 12.584558788691732 No Hit GCCTCCCTTAGGTCACTAGTTGCCTCATCCGGCACACTGGAGTTTAACAA 237126 12.483167207667435 No Hit GCCTCCCTTAGGTCACTAGTTGCCTCATCCGGCACCCTGGAGTTTAACAA 226335 11.915090078470556 No Hit GCCTCCCTTAGGTCACTAGTTGCCTCGTCAGGCACTCTGGAGTTTATCAC 218914 11.524421894264268 No Hit ATTGTTTGGCATAGTCACGTTCAATGCTGGGTATGTGTAGTTTAAGTGGG 6714 0.353449156280961 No Hit CTCCCTTAGGTCACTAGTTGCCTCGTCAGGCACTCTGGAGTTTATCACTG 5796 0.30512232794227734 No Hit TGGCATAGTCACGTTCAATGCTGGGTATGTGTAGTTTAAGTGGGTCAACC 5436 0.28617063055455827 No Hit TGGCATAGTCACGTTCAGCACTGGATATGTGCTTCCTGATTTGGTCAACC 5244 0.27606305861444136 No Hit CTCCCTTAGGTCACTAGTTGCCTCATCCGGCACCCTGGAGTTTAACAATG 5190 0.27322030400628355 No Hit CTCCCTTAGGTCACTAGTTGCCTCATCCGGCACACTGGAGTTTAACAATG 4718 0.24837252298682963 No Hit CATTGTTTGGCATAGTCACGTTCAATGCTGGGTATGTGTAGTTTAAGTGG 4404 0.23184243137643018 No Hit GCCTCCCTTAGGTCACTAGTTGCCTCGTCAGGCACTCTGGAGTTTAACAC 3955 0.2082054532456361 No Hit TCATTGTTTGGACTGGAGTCACTCAAAACGGAACAAGTTCTGCTTGCATA 3771 0.19851903013635747 No Hit CATTGTTTGGCATAGTCACGTTCAGTGCTGGATATTTGTATTTTAATTGG 3623 0.19072777676585073 No Hit CCTCCCTTAGGTCACTAGTTGCCTCGTCAGGCACTCTGGAGTTTATCACT 3620 0.1905698459542864 No Hit TCATTGTTTGGCATAGTCACTCAAAACGGAACAAGTTCTGCTTGCATAAG 3606 0.18983283550031954 No Hit GCCTCCCTTAGGTCACTAGTTGCCTCGTCAGGCACTCTGGAGTTTAACAA 3418 0.1799358379756218 No Hit TTGTTTGGCATAGTCACGTTCAATGCTGGGTATGTGTAGTTTAAGTGGGT 3351 0.17640871651735188 No Hit CATTGTTTGGCATAGTCACGTTCAGCACTGGATATGTGCTTCCTGATTTG 3178 0.16730137305047574 No Hit TGTTTGGCATAGTCACGTTCAATGCTGGGTATGTGTAGTTTAAGTGGGTC 3042 0.1601418429262263 No Hit TTGGCATAGTCACGTTCAATGCTGGGTATGTGTAGTTTAAGTGGGTCAAC 2967 0.15619357263711817 No Hit CCTCCCTTAGGTCACTAGTTGCCTCATCCGGCACACTGGAGTTTAACAAT 2882 0.15171886630946227 No Hit CCTCCCTTAGGTCACTAGTTGCCTCATCCGGCACCCTGGAGTTTAACAAT 2799 0.14734944718951593 No Hit GCCTCCCTTAGGTCACTAGTTGAACGAAGCAAAGCCTACAGCAACTGTTA 2789 0.14682301115096819 No Hit TTGGCATAGTCACGTTCAGCACTGGATATGTGCTTCCTGATTTGGTCAAC 2698 0.14203244320018363 No Hit TTGGCATAGTCACGTTCAGTGCTGGATATTTGTATTTTAATTGGTGCAAC 2597 0.13671543921085133 No Hit ATTGTTTGGCATAGTCACGTTCAGTGCTGGATATTTGTATTTTAATTGGT 2535 0.13345153577185526 No Hit TCCCTTAGGTCACTAGTTGCCTCATCCGGCACACTGGAGTTTAACAATGA 2508 0.13203015846777633 No Hit GGTCACTAGTTGCCTCGTCAGGCACTCTGGAGTTTATCACTGAGGGTTTC 2508 0.13203015846777633 No Hit GTCACGTTCAATGCTGGGTATGTGTAGTTTAAGTGGGTCAACCAATTTAA 2474 0.13024027593671397 No Hit TCCCTTAGGTCACTAGTTGCCTCATCCGGCACCCTGGAGTTTAACAATGA 2167 0.11407868955329796 No Hit GCCTCCCTTAGGTCACTAGTTGCCTCGTCAGGCACCCTGGAGTTTATCAC 2162 0.1138154715340241 No Hit CCCTTAGGTCACTAGTTGCCTCGTCAGGCACTCTGGAGTTTATCACTGAG 2118 0.11149915296441398 No Hit CCTTAGGTCACTAGTTGCCTCGTCAGGCACTCTGGAGTTTATCACTGAGG 2089 0.1099724884526255 No Hit TGTTTGGCATAGTCACGTTCAGTGCTGGATATTTGTATTTTAATTGGTGC 2073 0.10913019079094909 No Hit TCCCTTAGGTCACTAGTTGCCTCGTCAGGCACTCTGGAGTTTATCACTGA 2065 0.10870904196011089 No Hit CCCTTAGGTCACTAGTTGCCTCATCCGGCACCCTGGAGTTTAACAATGAA 1976 0.1040237612170359 No Hit GTTTGGCATAGTCACGTTCAATGCTGGGTATGTGTAGTTTAAGTGGGTCA 1952 0.10276031472452127 No Hit GCCTCCCTTAGGTCACTAGTTGCCTCATCCGGCACACTGGAGTTTATCAC 1910 0.10054928336262073 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0015793081156432575 0.0 0.0 2 0.0015793081156432575 0.0 0.0 3 0.0016319517194980327 0.0 0.0 4 0.0016319517194980327 0.0 0.0 5 0.0016319517194980327 0.0 0.0 6 0.0016319517194980327 0.0 0.0 7 0.0016319517194980327 0.0 0.0 8 0.0016319517194980327 0.0 0.0 9 0.0016319517194980327 0.0 0.0 10 0.0016319517194980327 0.0 0.0 11 0.0016319517194980327 0.0 0.0 12 0.0016319517194980327 0.0 0.0 13 0.001684595323352808 0.0 0.0 14 0.001684595323352808 0.0 0.0 15 0.0017898825310623584 0.0 0.0 16 0.0017898825310623584 0.0 0.0 17 0.0017898825310623584 0.0 0.0 18 0.0017898825310623584 0.0 0.0 19 0.0017898825310623584 0.0 0.0 20 0.0017898825310623584 0.0 0.0 21 0.0017898825310623584 0.0 0.0 22 0.0017898825310623584 0.0 0.0 23 0.0017898825310623584 0.0 0.0 24 0.0017898825310623584 0.0 0.0 25 0.0017898825310623584 0.0 0.0 26 0.0018425261349171336 0.0 0.0 27 0.0018425261349171336 0.0 0.0 28 0.0020004569464814595 0.0 0.0 29 0.0020004569464814595 0.0 0.0 30 0.002053100550336235 0.0 0.0 31 0.0021583877580457853 0.0 0.0 32 0.0023163185696101108 0.0 0.0 33 0.0024216057773196616 0.0 0.0 34 0.0026321801927387625 0.0 0.0 35 0.0027901110043030883 0.0 0.0 36 0.0030533290235769646 0.0 0.0 37 0.0032639034389960655 0.0 0.0 38 0.0034218342505603913 0.0 0.0 39 0.003948270289108144 0.0 0.0 40 0.0044747063276558965 0.0 0.0 41 0.004895855158494098 0.0 0.0 42 0.005369647593187075 0.0 0.0 43 0.006106658047153929 0.0 0.0 44 0.00642251967028258 0.0 0.0 45 0.0071595301242494335 0.0 0.0 46 0.00821240220134494 0.0 0.0 47 0.009265274278440443 0.0 0.0 48 0.009896997524697746 0.0 0.0 49 0.010791938790228927 0.0 0.0 50 0.012055385282743532 0.0 0.0 51 0.013002970152129487 0.0 0.0 52 0.01384526781380589 0.0 0.0 53 0.01563515034486825 0.0 0.0 54 0.01695124044123763 0.0 0.0 55 0.018267330537607013 0.0 0.0 56 0.019320202614702516 0.0 0.0 57 0.020636292711071896 0.0 0.0 58 0.02226824443056993 0.0 0.0 59 0.02416341416934184 0.0 0.0 60 0.026216514719678074 0.0 0.0 61 0.02779582283532133 0.0 0.0 62 0.029164556535545488 0.0 0.0 63 0.030743864651188745 0.0 0.0 64 0.03242845997454155 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTACTAT 55 0.0 70.00001 67 ACTCTTA 25 2.841707E-6 70.00001 69 GGACTCT 25 2.841707E-6 70.00001 65 GGGTAAA 35 3.7161954E-9 70.00001 50 TTACTAG 35 3.7161954E-9 70.00001 13 TAAGTGT 25 2.841707E-6 70.00001 45 ATGTATA 25 2.841707E-6 70.00001 35 GTGTGTC 25 2.841707E-6 70.00001 48 CATAGTA 55 0.0 70.00001 12 TGTCTCG 25 2.841707E-6 70.00001 21 TCTCGTA 70 0.0 70.00001 41 ATAGTAC 25 2.841707E-6 70.00001 13 GATTACG 25 2.841707E-6 70.00001 67 AGTGTGT 25 2.841707E-6 70.00001 47 TAGTACG 25 2.841707E-6 70.00001 14 CTAGATA 20 7.929946E-5 70.0 29 ATCCTAA 40 1.3460522E-10 70.0 41 AGGTATG 15 0.0022251287 70.0 31 GAGTGTA 15 0.0022251287 70.0 40 TTCTAAA 20 7.929946E-5 70.0 68 >>END_MODULE