##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename 000000000-AHV5L l01n01 kokuya2.34100000033cec.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2383239 Sequences flagged as poor quality 0 Sequence length 76 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 33.63158373960815 34.0 34.0 34.0 34.0 34.0 2 33.82202540324323 34.0 34.0 34.0 34.0 34.0 3 33.822527241288014 34.0 34.0 34.0 34.0 34.0 4 33.86818653101934 34.0 34.0 34.0 34.0 34.0 5 33.90805076620515 34.0 34.0 34.0 34.0 34.0 6 37.735920736443134 38.0 38.0 38.0 38.0 38.0 7 37.73342035775682 38.0 38.0 38.0 38.0 38.0 8 37.753623954626455 38.0 38.0 38.0 38.0 38.0 9 37.734812580693756 38.0 38.0 38.0 38.0 38.0 10-11 37.54740649175345 38.0 38.0 38.0 37.0 38.0 12-13 37.56440940249803 38.0 38.0 38.0 37.0 38.0 14-15 37.553777862816105 38.0 38.0 38.0 37.0 38.0 16-17 37.7274322885787 38.0 38.0 38.0 38.0 38.0 18-19 37.68499130804758 38.0 38.0 38.0 38.0 38.0 20-21 37.59717699315931 38.0 38.0 38.0 37.0 38.0 22-23 37.57753187993315 38.0 38.0 38.0 37.0 38.0 24-25 37.68061596004429 38.0 38.0 38.0 37.5 38.0 26-27 37.26810277945267 38.0 38.0 38.0 37.0 38.0 28-29 37.52991747785262 38.0 38.0 38.0 37.0 38.0 30-31 37.45779902896856 38.0 38.0 38.0 37.0 38.0 32-33 37.42627785127719 38.0 38.0 38.0 37.0 38.0 34-35 37.49176519853863 38.0 38.0 38.0 37.0 38.0 36-37 37.16659953114228 38.0 38.0 38.0 36.5 38.0 38-39 37.591399981285974 38.0 38.0 38.0 37.0 38.0 40-41 37.2373211415221 38.0 38.0 38.0 37.0 38.0 42-43 37.23542917852552 38.0 38.0 38.0 37.0 38.0 44-45 37.32252535310138 38.0 38.0 38.0 37.0 38.0 46-47 37.24654241559491 38.0 38.0 38.0 37.0 38.0 48-49 37.3686803967206 38.0 38.0 38.0 37.0 38.0 50-51 37.15487640979356 38.0 38.0 38.0 37.0 38.0 52-53 37.15750917134203 38.0 38.0 38.0 37.0 38.0 54-55 37.140322057502416 38.0 38.0 38.0 37.0 38.0 56-57 37.28971454394629 38.0 38.0 38.0 37.0 38.0 58-59 37.357165815094504 38.0 38.0 38.0 37.0 38.0 60-61 37.316136778560605 38.0 38.0 38.0 37.0 38.0 62-63 37.14037094055611 38.0 38.0 38.0 37.0 38.0 64-65 37.31412124423946 38.0 38.0 38.0 37.0 38.0 66-67 37.40970565688124 38.0 38.0 38.0 37.0 38.0 68-69 37.50039106442954 38.0 38.0 38.0 37.0 38.0 70-71 37.429228037976884 38.0 38.0 38.0 37.0 38.0 72-73 37.08302020905163 38.0 38.0 38.0 36.5 38.0 74-75 37.16270168455618 38.0 38.0 38.0 36.5 38.0 76 36.200377301647045 38.0 37.0 38.0 34.0 38.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.008339508523867778 1101 2 0.008551110784779326 1101 3 0.045300170989357014 1101 4 0.004241248029693168 1101 5 0.023827489827674242 1101 6 -0.033488734740885207 1101 7 -0.07224969655771218 1101 8 -0.0941489476674775 1101 9 -0.3790168672973664 1101 10-11 -0.5243621662443019 1101 12-13 -0.7373375730991967 1101 14-15 -0.2889315351696169 1101 16-17 -0.06981931852709522 1101 18-19 -0.0016359373327290427 1101 20-21 0.1132298598496817 1101 22-23 0.07329320214187618 1101 24-25 0.06802611051507768 1101 26-27 0.11999751921960922 1101 28-29 0.08945495457130193 1101 30-31 0.14005637576727992 1101 32-33 0.14299588574944977 1101 34-35 0.10914073543423086 1101 36-37 0.09614315029376996 1101 38-39 0.05833859792431184 1101 40-41 0.16191288502615464 1101 42-43 0.139354760160316 1101 44-45 0.16912808114505395 1101 46-47 0.14943392905612995 1101 48-49 0.12172285839097441 1101 50-51 0.19902518966018334 1101 52-53 0.2195102161828757 1101 54-55 0.19477149933999982 1101 56-57 0.13053972332559027 1101 58-59 0.1691233340366196 1101 60-61 0.16703327486111874 1101 62-63 0.1341200652656127 1101 64-65 0.14167625012208873 1101 66-67 0.16052369040580317 1101 68-69 0.07682447074582655 1101 70-71 0.09242178670519507 1101 72-73 0.12108475307020683 1101 74-75 0.15145397331934873 1101 76 0.15364129503095825 1102 1 0.04236092878045383 1102 2 -0.002021484851319144 1102 3 3.073112166447345E-4 1102 4 0.008939195833050917 1102 5 0.016815497764831377 1102 6 -0.03340182858522667 1102 7 0.019719571715484108 1102 8 -0.03729036922415929 1102 9 -0.20879815703514737 1102 10-11 -0.18648077607249292 1102 12-13 0.03529399846282644 1102 14-15 0.13204530865642994 1102 16-17 0.05154607337526329 1102 18-19 0.07536527354326239 1102 20-21 0.14934965868344108 1102 22-23 0.12948012837556888 1102 24-25 0.05920693084451045 1102 26-27 0.044058325683522526 1102 28-29 0.0544460115414509 1102 30-31 0.17367543695387155 1102 32-33 0.1564749181251841 1102 34-35 0.12107168168303417 1102 36-37 0.09951366206770729 1102 38-39 0.0771835167261159 1102 40-41 0.10511316213921873 1102 42-43 0.10762392773637686 1102 44-45 0.1581136782928496 1102 46-47 0.1146547213936131 1102 48-49 0.02838903192253639 1102 50-51 0.12951083035697764 1102 52-53 0.13279579671681319 1102 54-55 0.18312362159598905 1102 56-57 0.18057098871265254 1102 58-59 0.12128474302247128 1102 60-61 0.15576338819212054 1102 62-63 0.10893275111967426 1102 64-65 0.12997087344571412 1102 66-67 0.1991753196125714 1102 68-69 0.09602272843499549 1102 70-71 0.12391375738216226 1102 72-73 0.1922253071549136 1102 74-75 0.23651651807080754 1102 76 0.46579839461160333 1103 1 0.04800599379267112 1103 2 0.013975975567021237 1103 3 0.04422107486725224 1103 4 5.07244157759601E-4 1103 5 0.013378943043072411 1103 6 0.06714647724965062 1103 7 0.05390111744398496 1103 8 0.06668907469631336 1103 9 0.08614381000041504 1103 10-11 0.1758721855088936 1103 12-13 0.19062266079553325 1103 14-15 0.1644896735109711 1103 16-17 0.07169549484022042 1103 18-19 0.05418611671544937 1103 20-21 0.14041833692463968 1103 22-23 0.09721711140552003 1103 24-25 0.05675264752729703 1103 26-27 0.10011741866725288 1103 28-29 0.018121091940194844 1103 30-31 0.041925856907319314 1103 32-33 0.12460307529911319 1103 34-35 0.10877966546551932 1103 36-37 -0.006346530941812034 1103 38-39 0.06077857754180371 1103 40-41 0.10476138171279814 1103 42-43 0.052354377865405866 1103 44-45 -0.0013359225338902547 1103 46-47 0.09989529061969904 1103 48-49 -0.05706650116037082 1103 50-51 0.12008580136156155 1103 52-53 0.04991650333505504 1103 54-55 0.2141513719531858 1103 56-57 0.18891512522589693 1103 58-59 0.19235899766324138 1103 60-61 0.13211498390577248 1103 62-63 0.18646202201966133 1103 64-65 0.20706930291667902 1103 66-67 0.1691406739322474 1103 68-69 0.06783504625956738 1103 70-71 0.19139325322574052 1103 72-73 0.2682101801862231 1103 74-75 0.26137521225879823 1103 76 0.5538774945702087 2116 1 0.023230578555171633 2116 2 0.049916919762402756 2116 3 -0.0014187290067582126 2116 4 -0.010552272087267056 2116 5 0.0061128596960600134 2116 6 0.07149859686997218 2116 7 0.06197361641802246 2116 8 0.058683427124940124 2116 9 0.0796219013193209 2116 10-11 0.16572150255704798 2116 12-13 0.16113680132168184 2116 14-15 0.1323210447446641 2116 16-17 0.046123584657991046 2116 18-19 0.08400617831643586 2116 20-21 0.153060140248364 2116 22-23 0.14134968509176105 2116 24-25 0.06852436722145683 2116 26-27 0.1270584790567284 2116 28-29 0.07831636170399037 2116 30-31 0.12657591039667437 2116 32-33 0.04810548999044073 2116 34-35 0.16281866475161877 2116 36-37 0.13019955840755415 2116 38-39 0.11055175204982248 2116 40-41 0.1507495209127896 2116 42-43 0.13041047080269408 2116 44-45 0.10042500268951926 2116 46-47 0.20012591972597704 2116 48-49 0.16753806154117257 2116 50-51 0.23466350714326722 2116 52-53 0.1886426317447487 2116 54-55 0.25533248091037564 2116 56-57 0.15970173461550274 2116 58-59 0.18162089505863577 2116 60-61 0.21376390064686035 2116 62-63 0.2087688751172152 2116 64-65 0.20109643470664196 2116 66-67 0.21473817426060293 2116 68-69 0.03371292126094971 2116 70-71 0.14130247966883758 2116 72-73 0.3143932752008922 2116 74-75 0.21462338634556488 2116 76 0.3150737460836268 1117 1 0.02914406861138019 1117 2 -0.04609345458908365 1117 3 0.010093340369017767 1117 4 0.010605950597202707 1117 5 0.02647658866138869 1117 6 0.08063959645544116 1117 7 0.10424789638349807 1117 8 0.08001668333049139 1117 9 0.11279258202434761 1117 10-11 0.17426649333343391 1117 12-13 0.20241142600733752 1117 14-15 0.14603516214561552 1117 16-17 0.06648199446598824 1117 18-19 -2.04144156647601E-4 1117 20-21 0.15442385306229767 1117 22-23 0.07612149587393446 1117 24-25 -0.026938355556005433 1117 26-27 0.06942359471530324 1117 28-29 0.0898997133288546 1117 30-31 0.04901508867218496 1117 32-33 0.13358365577765596 1117 34-35 0.17691783927657667 1117 36-37 0.19901635036669063 1117 38-39 0.0951449513464766 1117 40-41 0.1226929606505962 1117 42-43 -0.1886317412265086 1117 44-45 -0.018640081716227996 1117 46-47 -0.12079636508592273 1117 48-49 -0.03843334084053396 1117 50-51 -0.14584638838709196 1117 52-53 0.03335858777337819 1117 54-55 -5.153023155628489E-4 1117 56-57 0.09289036193575129 1117 58-59 0.12099683822211205 1117 60-61 0.1518694101773903 1117 62-63 0.11061744297426657 1117 64-65 0.162331745832887 1117 66-67 0.16569003053985654 1117 68-69 0.177100531331142 1117 70-71 0.15267964468708328 1117 72-73 0.10834993952740746 1117 74-75 0.2409637842124397 1117 76 0.5307635259572265 2117 1 -0.04112610429157826 2117 2 -0.0012267265851590992 2117 3 0.007352410548634225 2117 4 -0.007112982310005123 2117 5 0.0014071647682527555 2117 6 -0.0495184892738223 2117 7 -0.0036573661238890054 2117 8 -0.11566067963028104 2117 9 -0.22937669399807703 2117 10-11 -0.304861525679911 2117 12-13 -0.7655134275736728 2117 14-15 -1.149847981662397 2117 16-17 -0.11927230162319802 2117 18-19 0.019774811121713753 2117 20-21 0.11890028178416401 2117 22-23 0.12378613988068565 2117 24-25 0.03436146293814346 2117 26-27 0.09945536769923535 2117 28-29 0.117109539331409 2117 30-31 0.09527887605201357 2117 32-33 0.10303963774211411 2117 34-35 0.15596667806624254 2117 36-37 0.17645571676698069 2117 38-39 0.11043859235749665 2117 40-41 0.20902937121037013 2117 42-43 0.20974410185232273 2117 44-45 0.2111436553786703 2117 46-47 0.040074421617305234 2117 48-49 0.17103019559720423 2117 50-51 0.22807419845460686 2117 52-53 0.23669814530836675 2117 54-55 0.22222515099779372 2117 56-57 0.11413188261936824 2117 58-59 0.16490192953790483 2117 60-61 0.17975459852404896 2117 62-63 0.16230681665376778 2117 64-65 0.17297863455182494 2117 66-67 0.20837758883177315 2117 68-69 0.2008348582117847 2117 70-71 0.19844637601942594 2117 72-73 0.1633136265665911 2117 74-75 0.24516662826297875 2117 76 0.3726357664729676 1116 1 0.05798028116577569 1116 2 0.0033474092665031208 1116 3 0.006833614008812106 1116 4 0.01583094136974239 1116 5 -0.007796909621127668 1116 6 -0.037180825101451376 1116 7 0.008722082143485466 1116 8 -0.1027348323259929 1116 9 -0.193161783129149 1116 10-11 -0.2591986006367719 1116 12-13 -0.033267674283052884 1116 14-15 0.10441864172413062 1116 16-17 0.0187550989627141 1116 18-19 0.045921901351235306 1116 20-21 0.16156686883103077 1116 22-23 0.0819908245678036 1116 24-25 0.039977593055006366 1116 26-27 0.06479882970005235 1116 28-29 0.080762158210689 1116 30-31 0.058449402105622994 1116 32-33 0.08794044697343395 1116 34-35 0.1023710235686579 1116 36-37 0.13217552468837823 1116 38-39 0.00671696614876538 1116 40-41 0.16717099940559876 1116 42-43 0.146966025711599 1116 44-45 0.19201455437026738 1116 46-47 0.03127370773414384 1116 48-49 0.1211593024738562 1116 50-51 0.16231168381930416 1116 52-53 0.16366867562188503 1116 54-55 0.1373514389422681 1116 56-57 0.14784643106968787 1116 58-59 0.12909176473361583 1116 60-61 0.1160725406035823 1116 62-63 0.04728031064957605 1116 64-65 0.13668925670992849 1116 66-67 0.13595637929034154 1116 68-69 0.1610521072512867 1116 70-71 0.16995527412991862 1116 72-73 0.10466307461445723 1116 74-75 0.19616749589422966 1116 76 0.3190001481237843 2118 1 -0.19390502781131147 2118 2 0.01846872628778584 2118 3 0.01997876607842386 2118 4 -0.023756556761291847 2118 5 0.0096954035888146 2118 6 0.06360051999261174 2118 7 0.04617779019520185 2118 8 0.03298322738780257 2118 9 0.09204128309576731 2118 10-11 0.17251233056123283 2118 12-13 0.19719257705600768 2118 14-15 0.14626609739309515 2118 16-17 0.06249918328052928 2118 18-19 0.06083931498050532 2118 20-21 0.1749016457618353 2118 22-23 0.1031669308634875 2118 24-25 0.08921911532663529 2118 26-27 0.06694732783206092 2118 28-29 0.06917882224424687 2118 30-31 0.09950624520823936 2118 32-33 0.09701259383794536 2118 34-35 0.129800556304545 2118 36-37 0.1019933029468092 2118 38-39 0.07658840019428226 2118 40-41 0.08420945078329822 2118 42-43 0.14842306020081963 2118 44-45 0.20568856856258577 2118 46-47 0.050349376998198636 2118 48-49 0.08860915198465591 2118 50-51 0.15580254680828176 2118 52-53 0.16120869649785874 2118 54-55 0.12294679015666787 2118 56-57 0.11478791330713989 2118 58-59 0.11009176353920935 2118 60-61 0.034524430804175665 2118 62-63 0.054033458522702915 2118 64-65 0.0647184985981255 2118 66-67 0.1380444515486161 2118 68-69 0.15293948580097805 2118 70-71 0.09688918168821203 2118 72-73 0.15251063495644956 2118 74-75 0.2333256214477899 2118 76 0.34899529668076923 1119 1 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0.09164848051327112 1119 60-61 0.1333125993114166 1119 62-63 0.14642536363628977 1119 64-65 0.12520614407935682 1119 66-67 0.1321736225324983 1119 68-69 0.04117399363203589 1119 70-71 0.10175145444574696 1119 72-73 0.13576974285076204 1119 74-75 0.1652247536605813 1119 76 0.02385762971402272 2119 1 0.02043549359797936 2119 2 -0.009562954160678316 2119 3 -0.04631826172041542 2119 4 0.029669212999742456 2119 5 0.038791088824922326 2119 6 0.10160280702552171 2119 7 0.085008299029667 2119 8 0.05883655256329945 2119 9 0.11607571480440271 2119 10-11 0.208898455945274 2119 12-13 0.2240395417567882 2119 14-15 0.17920850690634182 2119 16-17 0.04244887427638844 2119 18-19 0.08826610509180455 2119 20-21 0.16659816707647224 2119 22-23 0.11731335692736167 2119 24-25 -0.02123289703487785 2119 26-27 0.047783990767044315 2119 28-29 0.028990638875932007 2119 30-31 0.10827358692005618 2119 32-33 0.06514158178177354 2119 34-35 0.13427469967425765 2119 36-37 0.06141978342937193 2119 38-39 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0.05538868604625691 2104 44-45 0.03330777866084844 2104 46-47 0.032086107024028365 2104 48-49 -0.03224154792680878 2104 50-51 -0.07246748281709614 2104 52-53 -0.03480047867768832 2104 54-55 -0.014290583467392537 2104 56-57 -0.006054662643514064 2104 58-59 0.1025851205893531 2104 60-61 0.020347474431908097 2104 62-63 -0.02723039250809478 2104 64-65 -0.18382139353114724 2104 66-67 -0.09838625145253133 2104 68-69 0.02354223727045479 2104 70-71 -0.06577493843911952 2104 72-73 -0.09507724502247328 2104 74-75 -0.02768888330395214 2104 76 -0.3177902119268907 2111 1 0.03837054231335202 2111 2 0.017102872256508306 2111 3 0.022398562747646622 2111 4 0.004952969067680613 2111 5 -0.02833585632079405 2111 6 -0.1570412594764008 2111 7 -0.05759387721647613 2111 8 -0.06370005876148355 2111 9 -0.05435618615819493 2111 10-11 -0.3537273873278224 2111 12-13 -0.4134249417889606 2111 14-15 -0.25366891469901276 2111 16-17 -0.10330223873589262 2111 18-19 -0.05651524544372677 2111 20-21 0.09201505176241653 2111 22-23 -0.06555829751113151 2111 24-25 -0.00906430225028032 2111 26-27 -0.057770351094816874 2111 28-29 -0.015373349559176575 2111 30-31 -0.08134516897601429 2111 32-33 -0.058314618941096796 2111 34-35 -0.04618933446533191 2111 36-37 0.038006155346700154 2111 38-39 -0.01312643240741096 2111 40-41 -0.08441617531273238 2111 42-43 -0.04466254732723485 2111 44-45 1.984848538754136E-4 2111 46-47 0.006402001539257185 2111 48-49 0.03568270943267038 2111 50-51 -0.030628314233744902 2111 52-53 -0.11780024745819162 2111 54-55 -0.02002653079802741 2111 56-57 -0.11062721080410398 2111 58-59 -0.29176935107409463 2111 60-61 -0.10151359915673908 2111 62-63 -0.07215999225497427 2111 64-65 -0.08678684447002638 2111 66-67 -0.08324575518246746 2111 68-69 -0.14823182476938968 2111 70-71 -0.05751456094286311 2111 72-73 -0.09057394101751726 2111 74-75 -0.015036308857354186 2111 76 -0.10680704227262794 2110 1 0.024784394160427325 2110 2 -0.0012677176208768515 2110 3 -0.0012238756412656926 2110 4 -0.009043609486006687 2110 5 0.038261951949372985 2110 6 -0.10347200381992394 2110 7 -0.008268503398412008 2110 8 -0.06897209237468616 2110 9 -0.0884708690302034 2110 10-11 -0.3252444194020967 2110 12-13 -0.3777804858919751 2110 14-15 -0.14190085726452395 2110 16-17 -0.07138680142780629 2110 18-19 -0.081573044981333 2110 20-21 -0.02901581999060454 2110 22-23 -0.04372066589639445 2110 24-25 -0.030962990218675657 2110 26-27 0.029877082462554938 2110 28-29 1.1112867025531159E-4 2110 30-31 -0.07797411383918984 2110 32-33 -0.13811555948093712 2110 34-35 -0.0031328662855045764 2110 36-37 -0.015191823279991468 2110 38-39 0.05303059623110329 2110 40-41 -0.039293311032487566 2110 42-43 0.06083209503141518 2110 44-45 -0.056795008671855385 2110 46-47 0.02365890348404065 2110 48-49 -0.05009652854395341 2110 50-51 0.03770183386191661 2110 52-53 -0.10860128104992128 2110 54-55 -0.09008236193032104 2110 56-57 -0.13863217834330754 2110 58-59 -0.05890120559820389 2110 60-61 -0.12169719768795062 2110 62-63 -0.2664075961688823 2110 64-65 -0.1818674775091651 2110 66-67 -0.25305693142990293 2110 68-69 -0.2746123210938407 2110 70-71 -0.2500705531265055 2110 72-73 -0.18850997858184115 2110 74-75 -0.14822219073872134 2110 76 -0.3653207888994956 2109 1 0.030484898560025897 2109 2 0.02047227190048062 2109 3 -0.011688988132249278 2109 4 -0.012580823031981936 2109 5 0.012509258317280114 2109 6 -0.17761439631790665 2109 7 -0.05325200711941136 2109 8 -0.08806083602436843 2109 9 -0.19425087681198505 2109 10-11 -0.4150156074500444 2109 12-13 -0.48124394670125525 2109 14-15 -0.1259155854325016 2109 16-17 -0.07473775638197822 2109 18-19 -0.09411196629904595 2109 20-21 0.026115873332287265 2109 22-23 -0.04268073389565785 2109 24-25 -0.033826981645397325 2109 26-27 -0.07633857920798448 2109 28-29 -0.10745306645112862 2109 30-31 -0.0331800202336936 2109 32-33 -0.08338623429086311 2109 34-35 -0.1509172318101335 2109 36-37 -0.1113471839084994 2109 38-39 -0.058901929433993416 2109 40-41 -0.2839407136033145 2109 42-43 -0.1392553119472879 2109 44-45 -0.22280739777186653 2109 46-47 -0.09649325632951644 2109 48-49 -0.23872719078743643 2109 50-51 -0.11417051823848823 2109 52-53 -0.5251017668094988 2109 54-55 -0.2574443113821445 2109 56-57 -0.04661197091497371 2109 58-59 -0.012551144543628823 2109 60-61 -0.10516657061405255 2109 62-63 -0.01645341719887483 2109 64-65 -0.05906672760232823 2109 66-67 -0.15647841677630225 2109 68-69 0.022614253063927947 2109 70-71 -0.14291170060974423 2109 72-73 -0.3119789931549164 2109 74-75 -0.270934096005476 2109 76 -0.4832347294981574 2108 1 -0.011019310934322846 2108 2 0.012204551424567 2108 3 -0.0340325325319597 2108 4 0.006057324286516064 2108 5 -0.004125632084956976 2108 6 -0.004671376167998176 2108 7 4.4167185876631265E-5 2108 8 0.033652301077665925 2108 9 -0.021418592666805125 2108 10-11 0.0916721728815304 2108 12-13 0.11610222945318327 2108 14-15 0.07771660521174084 2108 16-17 0.027161586151379424 2108 18-19 0.0035111563441390103 2108 20-21 0.08252827238594307 2108 22-23 -0.09546911973311722 2108 24-25 -0.015034478274159824 2108 26-27 -0.029110170084734932 2108 28-29 -0.0385271794326556 2108 30-31 -0.12483433025291646 2108 32-33 -0.1283295775627522 2108 34-35 -0.028748621904036042 2108 36-37 -0.11608902740317717 2108 38-39 -0.022740306259954934 2108 40-41 -0.07082011549260159 2108 42-43 0.01086292358237273 2108 44-45 -7.104397395991668E-4 2108 46-47 0.010489507682336807 2108 48-49 -0.04619805923243092 2108 50-51 -0.004964054577897059 2108 52-53 -0.12351207337857772 2108 54-55 -0.03744661696254781 2108 56-57 -0.09718088068849084 2108 58-59 -0.06267547765200732 2108 60-61 -0.1206905162457943 2108 62-63 -0.07610232443681042 2108 64-65 -0.10056011194237158 2108 66-67 -0.05061486724299158 2108 68-69 -0.1853811491203743 2108 70-71 -0.01255579580039523 2108 72-73 0.01016286974695646 2108 74-75 -0.038808679529850565 2108 76 -0.08279275087006965 >>END_MODULE >>Per sequence quality scores pass #Quality Count 14 4.0 15 17.0 16 63.0 17 47.0 18 60.0 19 90.0 20 94.0 21 194.0 22 336.0 23 499.0 24 713.0 25 918.0 26 1173.0 27 1729.0 28 2617.0 29 4098.0 30 6371.0 31 11878.0 32 21296.0 33 33028.0 34 58519.0 35 102757.0 36 212541.0 37 1924197.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 50.457801336752205 1.7876511755640117 42.782154874102005 4.972392613581769 2 2.31550423604179 1.7382226457354888 4.521367768822179 91.42490534940055 3 2.6338944604380843 40.351764971956236 4.160430405846833 52.853910161758854 4 2.8751627511970055 1.2492242699955816 90.90741633549972 4.9681966433077 5 2.598186753405764 1.6544710790650876 42.67679406052016 53.07054810700899 6 41.370462635094505 1.98813463525899 5.339959609590142 51.301443120056355 7 2.740430145696676 2.3268333557817744 44.58654797105955 50.346188527462 8 3.801087511575633 3.338481788859615 90.8451061769298 2.015324522634952 9 5.526008931542325 2.5973895190536913 89.6943613292666 2.1822402201373845 10-11 67.07975994014868 26.51817547463767 3.0639184739759626 3.3381461112376893 12-13 26.310663764733626 22.931208326147733 27.196034472413384 23.562093436705254 14-15 2.992901677087359 46.33876417765906 23.06625562941862 27.60207851583496 16-17 22.471350963961232 3.2081549521470567 46.95599140497449 27.364502678917223 18-19 27.49455677756196 45.54488660180536 4.541844103759631 22.418712516873047 20-21 23.150258954305464 1.5688942653254667 52.00682768282996 23.274019097539107 22-23 26.298390551681976 2.2049823790228342 41.956094206246206 29.540532863048984 24-25 3.320627935343455 22.655239361222268 26.93191912351216 47.09221357992212 26-27 13.681779292802778 35.44474557524445 20.049185163552625 30.824289968400148 28-29 20.849503553777023 4.905152189939826 26.73177134143911 47.51357291484404 30-31 48.97603639416777 5.192743992524459 21.480199845672214 24.35101976763556 32-33 57.54844562379182 11.372841750239905 3.167076403163929 27.911636222804344 34-35 3.15788722826372 46.283167571527656 22.856352216458358 27.702592983750268 36-37 18.950218589071426 17.71301577391105 31.642525151694816 31.694240485322705 38-39 48.200495208411745 3.3030468198951093 46.68604365739231 1.8104143143008318 40-41 28.196815342481386 43.03594813612903 24.00168426246801 4.765552258921577 42-43 38.092213999519146 2.632027253666124 54.26363029473754 5.012128452077194 44-45 2.765731846449307 5.281467783969631 86.64168805562514 5.311112313955923 46-47 5.88447067205597 85.05865337047607 5.476223744240507 3.5806522132274607 48-49 15.272681422215733 28.728402816503085 29.808739282967423 26.19017647831376 50-51 39.91897161803747 24.818639674829086 29.555743255292484 5.706645451840961 52-53 45.98105771179475 29.563589719704993 21.390699799726338 3.0646527687739247 54-55 8.945619805651049 65.06926078332891 3.305942039384216 22.679177371635827 56-57 25.648203977863737 22.211431585334076 7.466917921366678 44.67344651543551 58-59 1.8747595184536674 23.553428758089307 51.82780661108685 22.744005112370182 60-61 5.077249910730732 44.27247959604555 24.297919763817223 26.352350729406492 62-63 3.423618025720459 24.427847983353747 59.28364717092999 12.864886819995814 64-65 31.1399528121183 39.280009264702365 26.930828171240904 2.649209751938433 66-67 26.82485894196931 27.588630431106576 22.59190118993521 22.994609436988906 68-69 4.709515075911396 22.695038139271805 45.80621163047432 26.78923515434247 70-71 51.63137226270634 5.069550305277818 21.773015631248064 21.526061800767778 72-73 32.534147855082935 60.47007874577414 3.404358522162486 3.5914148769804455 74-75 18.8229128509562 26.529609493634503 26.3598405363457 28.28763711906359 76 12.914357309527077 79.07129750729995 3.5134537492882583 4.500891433884726 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.5 12 1.0 13 0.5 14 0.0 15 0.0 16 0.0 17 0.0 18 1.5 19 2.5 20 1.5 21 1.0 22 4.5 23 7.0 24 10.0 25 14.0 26 14.5 27 59.5 28 221.0 29 338.0 30 961.0 31 1950.0 32 10053.0 33 17790.0 34 106882.5 35 125123.5 36 295998.5 37 537725.0 38 295771.0 39 32857.5 40 11671.0 41 67775.0 42 124106.0 43 70220.5 44 15006.0 45 12676.5 46 11676.0 47 34281.5 48 367683.5 49 397374.5 50 116269.0 51 175707.0 52 136430.0 53 78669.0 54 119623.0 55 68175.5 56 10352.5 57 3061.5 58 2146.0 59 2080.0 60 2218.0 61 2751.0 62 3080.0 63 3457.5 64 4271.5 65 5107.0 66 5749.5 67 5993.0 68 6122.0 69 6169.0 70 5808.5 71 5530.0 72 5251.0 73 4435.0 74 3481.0 75 3064.0 76 2674.0 77 1912.5 78 1220.5 79 900.0 80 691.0 81 387.5 82 228.5 83 164.0 84 121.0 85 56.0 86 22.0 87 10.0 88 9.5 89 5.0 90 2.0 91 1.5 92 0.0 93 1.0 94 1.5 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 2383239.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 4.434645886264148 #Duplication Level Percentage of deduplicated Percentage of total 1 86.85168406301017 3.8515646344514156 2 5.774167942048642 0.5121278022160867 3 2.443955402950562 0.3251423032172325 4 1.1732113372023787 0.2081110732097196 5 0.7115973806830429 0.15778411984611998 6 0.3830647632958458 0.10192539460336036 7 0.3859656476321782 0.11981346800579219 8 0.19381422030248624 0.06875979478111313 9 0.14120998099532767 0.05635946351883317 >10 1.1875324551418391 1.1332828657251615 >50 0.2631375124948621 0.8301817976002425 >100 0.34932466863394496 3.474068133314872 >500 0.06260459926295632 1.9752057662617346 >1k 0.063553153797224 5.057647374151554 >5k 0.007588436274295402 2.2436648172937406 >10k+ 0.007588436274295402 79.88436119180302 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GCCTCCCTTAGGTCACTAGTTGCCTCATCCGGCACACTGGAGTTTAACAA 698544 29.3106985912869 No Hit TCATTGTTTGGCATAGTCACGTTCAATGCTGGGTATGTGTAGTTTAAGTG 508797 21.34897087535073 No Hit GCCTCCCTTAGGTCACTAGTTGCCTCATCCGGCACCCTGGAGTTTAACAA 234939 9.857970602193065 No Hit TCATTGTTTGGCATAGTCACGTTCAGTGCTGGATATTTGTATTTTAATTG 192361 8.071410378900312 No Hit TCATTGTTTGGCATAGTCACGTTCAGCACTGGATATGTGCTTCCTGATTT 121737 5.108048332542393 No Hit GCCTCCCTTAGGTCACTAGTTGCCTCGTCAGGCACTCTGGAGTTTATCAC 112440 4.7179489761622735 No Hit CTCCCTTAGGTCACTAGTTGCCTCATCCGGCACACTGGAGTTTAACAATG 20191 0.8472083580371083 No Hit ATTGTTTGGCATAGTCACGTTCAATGCTGGGTATGTGTAGTTTAAGTGGG 10059 0.42207264986851933 No Hit CCTCCCTTAGGTCACTAGTTGCCTCATCCGGCACACTGGAGTTTAACAAT 8409 0.35283914034639413 No Hit TCCCTTAGGTCACTAGTTGCCTCATCCGGCACACTGGAGTTTAACAATGA 7766 0.3258590514841357 No Hit CTCCCTTAGGTCACTAGTTGCCTCATCCGGCACCCTGGAGTTTAACAATG 7417 0.3112151152276377 No Hit CATTGTTTGGCATAGTCACGTTCAATGCTGGGTATGTGTAGTTTAAGTGG 6499 0.2726961081116917 No Hit TTGGCATAGTCACGTTCAATGCTGGGTATGTGTAGTTTAAGTGGGTCAAC 6380 0.2677029034855506 No Hit TGGCATAGTCACGTTCAATGCTGGGTATGTGTAGTTTAAGTGGGTCAACC 5875 0.2465132536015062 No Hit CCCTTAGGTCACTAGTTGCCTCATCCGGCACACTGGAGTTTAACAATGAA 5586 0.2343868995094491 No Hit TCATTGTTTGGCATAGTCACTCAAAACGGAACAAGTTCTGCTTGCATAAG 5406 0.22683415301612636 No Hit TGTTTGGCATAGTCACGTTCAATGCTGGGTATGTGTAGTTTAAGTGGGTC 4405 0.1848324905727038 No Hit CTCCCTTAGGTCACTAGTTGCCTCGTCAGGCACTCTGGAGTTTATCACTG 4128 0.1732096529135349 No Hit TTGTTTGGCATAGTCACGTTCAATGCTGGGTATGTGTAGTTTAAGTGGGT 3782 0.15869159576525896 No Hit GCCTCCCTTAGGTCACTAGTTGCCTCATCCGGCACACTGGAGTTTAACAC 3559 0.1493345820540869 No Hit CCTCCCTTAGGTCACTAGTTGCCTCATCCGGCACCCTGGAGTTTAACAAT 3261 0.1368305906373637 No Hit TCCCTTAGGTCACTAGTTGCCTCATCCGGCACCCTGGAGTTTAACAATGA 3041 0.12759945603441367 No Hit TCATTGTTTGGCATAGTCACGTTCAGTGCTGGGTATGTGTAGTTTAAGTG 2842 0.11924947518901798 No Hit GCTCCCTTAGGTCACTAGTTGCCTCATCCGGCACACTGGAGTTTAACAAT 2761 0.11585073926702273 No Hit CCTTAGGTCACTAGTTGCCTCATCCGGCACACTGGAGTTTAACAATGAAA 2660 0.11161280929021386 No Hit GCCTCCCTTAGGTCACTAGTTGCCTCATCCGGCACACTGGAGTTTATCAC 2616 0.10976658236962386 No Hit CATTGTTTGGCATAGTCACGTTCAGTGCTGGATATTTGTATTTTAATTGG 2543 0.10670352406955408 No Hit TCATTGTTTGGACTGGAGTCACTCAAAACGGAACAAGTTCTGCTTGCATA 2459 0.10317890903933681 No Hit TTTGGCATAGTCACGTTCAATGCTGGGTATGTGTAGTTTAAGTGGGTCAA 2440 0.10238167468726385 No Hit GCCTCCCTTAGGTCACTAGTTGCCTCGTCAGGCACTCTGGAGTTTAACAA 2388 0.10019977014474837 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0010070328657763657 0.0 0.0 2 0.0010070328657763657 0.0 0.0 3 0.001300750784961139 0.0 0.0 4 0.001300750784961139 0.0 0.0 5 0.001300750784961139 0.0 0.0 6 0.001300750784961139 0.0 0.0 7 0.001300750784961139 0.0 0.0 8 0.001300750784961139 0.0 0.0 9 0.001300750784961139 0.0 0.0 10 0.001300750784961139 0.0 0.0 11 0.001300750784961139 0.0 0.0 12 0.001300750784961139 0.0 0.0 13 0.001300750784961139 0.0 0.0 14 0.001300750784961139 0.0 0.0 15 0.001300750784961139 0.0 0.0 16 0.001300750784961139 0.0 0.0 17 0.001300750784961139 0.0 0.0 18 0.001300750784961139 0.0 0.0 19 0.001300750784961139 0.0 0.0 20 0.001300750784961139 0.0 0.0 21 0.001300750784961139 0.0 0.0 22 0.001300750784961139 0.0 0.0 23 0.001300750784961139 0.0 0.0 24 0.001300750784961139 0.0 0.0 25 0.001342710487701821 0.0 0.0 26 0.001342710487701821 0.0 0.0 27 0.001342710487701821 0.0 0.0 28 0.0013846701904425029 0.0 0.0 29 0.0014685895959238666 0.0 0.0 30 0.0015105492986645485 0.0 0.0 31 0.0015944687041459123 0.0 0.0 32 0.0015944687041459123 0.0 0.0 33 0.0015944687041459123 0.0 0.0 34 0.0015944687041459123 0.0 0.0 35 0.0015944687041459123 0.0 0.0 36 0.0016364284068865942 0.0 0.0 37 0.001678388109627276 0.0 0.0 38 0.00176230751510864 0.0 0.0 39 0.0019301463260713676 0.0 0.0 40 0.0020140657315527314 0.0 0.0 41 0.002139944839774777 0.0 0.0 42 0.0022658239479968227 0.0 0.0 43 0.0023917030562188683 0.0 0.0 44 0.0023917030562188683 0.0 0.0 45 0.0025175821644409144 0.0 0.0 46 0.00264346127266296 0.0 0.0 47 0.0028113000836256876 0.0 0.0 48 0.0028113000836256876 0.0 0.0 49 0.003063058300069779 0.0 0.0 50 0.003105018002810461 0.0 0.0 51 0.003356776219254552 0.0 0.0 52 0.0034826553274765982 0.0 0.0 53 0.0038183329494020533 0.0 0.0 54 0.003986171760364781 0.0 0.0 55 0.004363809085030918 0.0 0.0 56 0.004909285220659782 0.0 0.0 57 0.005119083734363193 0.0 0.0 58 0.00528692254532592 0.0 0.0 59 0.005664559869992057 0.0 0.0 60 0.006084156897398876 0.0 0.0 61 0.006503753924805695 0.0 0.0 62 0.0069233509522125146 0.0 0.0 63 0.007342947979619333 0.0 0.0 64 0.007804504709766834 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GATACGT 35 3.7161954E-9 70.00001 32 GGTACAC 65 0.0 70.00001 31 CATATTC 35 3.7161954E-9 70.00001 12 ACTCTCC 35 3.7161954E-9 70.00001 67 GGTCCTA 45 5.456968E-12 70.00001 11 ATTGTAC 35 3.7161954E-9 70.00001 62 AAGGTTT 140 0.0 70.00001 53 TGTATAG 45 5.456968E-12 70.00001 36 ATGTATA 45 5.456968E-12 70.00001 35 TATCTAC 115 0.0 70.00001 64 TGACATA 45 5.456968E-12 70.00001 9 GGAGTAT 45 5.456968E-12 70.00001 39 TTAACAT 45 5.456968E-12 70.00001 44 CCTATTT 115 0.0 70.00001 57 GTAACGT 45 5.456968E-12 70.00001 16 AATGTGT 35 3.7161954E-9 70.00001 34 AGTAACG 45 5.456968E-12 70.00001 15 CTCAGGT 35 3.7161954E-9 70.00001 7 GTAAACC 90 0.0 70.00001 52 TAGTAAC 45 5.456968E-12 70.00001 14 >>END_MODULE