FastQCFastQC Report
Tue 25 Aug 2015
000000000-AHV5L l01n01 kokuya2.34100000033cec.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AHV5L l01n01 kokuya2.34100000033cec.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2383239
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTCCCTTAGGTCACTAGTTGCCTCATCCGGCACACTGGAGTTTAACAA69854429.3106985912869No Hit
TCATTGTTTGGCATAGTCACGTTCAATGCTGGGTATGTGTAGTTTAAGTG50879721.34897087535073No Hit
GCCTCCCTTAGGTCACTAGTTGCCTCATCCGGCACCCTGGAGTTTAACAA2349399.857970602193065No Hit
TCATTGTTTGGCATAGTCACGTTCAGTGCTGGATATTTGTATTTTAATTG1923618.071410378900312No Hit
TCATTGTTTGGCATAGTCACGTTCAGCACTGGATATGTGCTTCCTGATTT1217375.108048332542393No Hit
GCCTCCCTTAGGTCACTAGTTGCCTCGTCAGGCACTCTGGAGTTTATCAC1124404.7179489761622735No Hit
CTCCCTTAGGTCACTAGTTGCCTCATCCGGCACACTGGAGTTTAACAATG201910.8472083580371083No Hit
ATTGTTTGGCATAGTCACGTTCAATGCTGGGTATGTGTAGTTTAAGTGGG100590.42207264986851933No Hit
CCTCCCTTAGGTCACTAGTTGCCTCATCCGGCACACTGGAGTTTAACAAT84090.35283914034639413No Hit
TCCCTTAGGTCACTAGTTGCCTCATCCGGCACACTGGAGTTTAACAATGA77660.3258590514841357No Hit
CTCCCTTAGGTCACTAGTTGCCTCATCCGGCACCCTGGAGTTTAACAATG74170.3112151152276377No Hit
CATTGTTTGGCATAGTCACGTTCAATGCTGGGTATGTGTAGTTTAAGTGG64990.2726961081116917No Hit
TTGGCATAGTCACGTTCAATGCTGGGTATGTGTAGTTTAAGTGGGTCAAC63800.2677029034855506No Hit
TGGCATAGTCACGTTCAATGCTGGGTATGTGTAGTTTAAGTGGGTCAACC58750.2465132536015062No Hit
CCCTTAGGTCACTAGTTGCCTCATCCGGCACACTGGAGTTTAACAATGAA55860.2343868995094491No Hit
TCATTGTTTGGCATAGTCACTCAAAACGGAACAAGTTCTGCTTGCATAAG54060.22683415301612636No Hit
TGTTTGGCATAGTCACGTTCAATGCTGGGTATGTGTAGTTTAAGTGGGTC44050.1848324905727038No Hit
CTCCCTTAGGTCACTAGTTGCCTCGTCAGGCACTCTGGAGTTTATCACTG41280.1732096529135349No Hit
TTGTTTGGCATAGTCACGTTCAATGCTGGGTATGTGTAGTTTAAGTGGGT37820.15869159576525896No Hit
GCCTCCCTTAGGTCACTAGTTGCCTCATCCGGCACACTGGAGTTTAACAC35590.1493345820540869No Hit
CCTCCCTTAGGTCACTAGTTGCCTCATCCGGCACCCTGGAGTTTAACAAT32610.1368305906373637No Hit
TCCCTTAGGTCACTAGTTGCCTCATCCGGCACCCTGGAGTTTAACAATGA30410.12759945603441367No Hit
TCATTGTTTGGCATAGTCACGTTCAGTGCTGGGTATGTGTAGTTTAAGTG28420.11924947518901798No Hit
GCTCCCTTAGGTCACTAGTTGCCTCATCCGGCACACTGGAGTTTAACAAT27610.11585073926702273No Hit
CCTTAGGTCACTAGTTGCCTCATCCGGCACACTGGAGTTTAACAATGAAA26600.11161280929021386No Hit
GCCTCCCTTAGGTCACTAGTTGCCTCATCCGGCACACTGGAGTTTATCAC26160.10976658236962386No Hit
CATTGTTTGGCATAGTCACGTTCAGTGCTGGATATTTGTATTTTAATTGG25430.10670352406955408No Hit
TCATTGTTTGGACTGGAGTCACTCAAAACGGAACAAGTTCTGCTTGCATA24590.10317890903933681No Hit
TTTGGCATAGTCACGTTCAATGCTGGGTATGTGTAGTTTAAGTGGGTCAA24400.10238167468726385No Hit
GCCTCCCTTAGGTCACTAGTTGCCTCGTCAGGCACTCTGGAGTTTAACAA23880.10019977014474837No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATACGT353.7161954E-970.0000132
GGTACAC650.070.0000131
CATATTC353.7161954E-970.0000112
ACTCTCC353.7161954E-970.0000167
GGTCCTA455.456968E-1270.0000111
ATTGTAC353.7161954E-970.0000162
AAGGTTT1400.070.0000153
TGTATAG455.456968E-1270.0000136
ATGTATA455.456968E-1270.0000135
TATCTAC1150.070.0000164
TGACATA455.456968E-1270.000019
GGAGTAT455.456968E-1270.0000139
TTAACAT455.456968E-1270.0000144
CCTATTT1150.070.0000157
GTAACGT455.456968E-1270.0000116
AATGTGT353.7161954E-970.0000134
AGTAACG455.456968E-1270.0000115
CTCAGGT353.7161954E-970.000017
GTAAACC900.070.0000152
TAGTAAC455.456968E-1270.0000114
Produced by FastQC (version 0.11.2)