##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename 000000000-AHV5L l01n01 kokuya1.34100000033cdf.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2044449 Sequences flagged as poor quality 0 Sequence length 76 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 33.594285306212086 34.0 34.0 34.0 34.0 34.0 2 33.78899693756117 34.0 34.0 34.0 34.0 34.0 3 33.794168991253876 34.0 34.0 34.0 34.0 34.0 4 33.843342142552835 34.0 34.0 34.0 34.0 34.0 5 33.89274665203192 34.0 34.0 34.0 34.0 34.0 6 37.71465857059775 38.0 38.0 38.0 37.0 38.0 7 37.72290578048168 38.0 38.0 38.0 38.0 38.0 8 37.72399947369682 38.0 38.0 38.0 38.0 38.0 9 37.68355825946257 38.0 38.0 38.0 38.0 38.0 10-11 37.49913081715415 38.0 38.0 38.0 37.0 38.0 12-13 37.503566486618155 38.0 38.0 38.0 37.0 38.0 14-15 37.501501627088764 38.0 38.0 38.0 37.0 38.0 16-17 37.685353119593586 38.0 38.0 38.0 37.0 38.0 18-19 37.66316009839326 38.0 38.0 38.0 37.0 38.0 20-21 37.56938104105312 38.0 38.0 38.0 37.0 38.0 22-23 37.54458022675058 38.0 38.0 38.0 37.0 38.0 24-25 37.65254110031603 38.0 38.0 38.0 37.0 38.0 26-27 37.217588210808884 38.0 38.0 38.0 37.0 38.0 28-29 37.490067494958296 38.0 38.0 38.0 37.0 38.0 30-31 37.4377749212624 38.0 38.0 38.0 37.0 38.0 32-33 37.35977444289391 38.0 38.0 38.0 37.0 38.0 34-35 37.46760985478239 38.0 38.0 38.0 37.0 38.0 36-37 37.1626858874934 38.0 38.0 38.0 37.0 38.0 38-39 37.578106374871666 38.0 38.0 38.0 37.0 38.0 40-41 37.208634698150945 38.0 38.0 38.0 37.0 38.0 42-43 37.193241553103064 38.0 38.0 38.0 37.0 38.0 44-45 37.35705243808968 38.0 38.0 38.0 37.0 38.0 46-47 37.26696704099736 38.0 38.0 38.0 37.0 38.0 48-49 37.336499712147386 38.0 38.0 38.0 37.0 38.0 50-51 37.09346087870131 38.0 38.0 38.0 36.5 38.0 52-53 37.05408156427478 38.0 38.0 38.0 36.5 38.0 54-55 37.10892470293952 38.0 38.0 38.0 37.0 38.0 56-57 37.29747990778934 38.0 38.0 38.0 37.0 38.0 58-59 37.31034841172365 38.0 38.0 38.0 37.0 38.0 60-61 37.2647510405004 38.0 38.0 38.0 37.0 38.0 62-63 37.09822035179161 38.0 38.0 38.0 37.0 38.0 64-65 37.25902675977733 38.0 38.0 38.0 37.0 38.0 66-67 37.366837959763245 38.0 38.0 38.0 37.0 38.0 68-69 37.4493729117234 38.0 38.0 38.0 37.0 38.0 70-71 37.41119220875649 38.0 38.0 38.0 37.0 38.0 72-73 37.088224259935075 38.0 38.0 38.0 36.5 38.0 74-75 37.189925256144804 38.0 38.0 38.0 37.0 38.0 76 36.15197542222868 38.0 37.0 38.0 34.0 38.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.02472120074392592 1101 2 0.007765361162498152 1101 3 0.05508273293603594 1101 4 0.012491898856303862 1101 5 0.02749675720087197 1101 6 6.358982110725719E-4 1101 7 -0.05747610493448008 1101 8 -0.11282760853702456 1101 9 -0.48489139370577305 1101 10-11 -0.6016428944288847 1101 12-13 -0.881092290223549 1101 14-15 -0.34960215565532593 1101 16-17 -0.06477986368149402 1101 18-19 0.02349838918149061 1101 20-21 0.14679774924245237 1101 22-23 0.0844447649250526 1101 24-25 0.06691441281279253 1101 26-27 0.0989272898535205 1101 28-29 0.07220074929340825 1101 30-31 0.14386015099051264 1101 32-33 0.16583412304940026 1101 34-35 0.12629109408411665 1101 36-37 0.11412042631401675 1101 38-39 0.00775584210693836 1101 40-41 0.14662130788747874 1101 42-43 0.15485333681640867 1101 44-45 0.12801946961277366 1101 46-47 0.10673537561980595 1101 48-49 0.12985441917012253 1101 50-51 0.19536948013043087 1101 52-53 0.23574049651514173 1101 54-55 0.17039635917996065 1101 56-57 0.13123694772539096 1101 58-59 0.1461469851291426 1101 60-61 0.09635345918904648 1101 62-63 0.07492096740833887 1101 64-65 0.1292175027402962 1101 66-67 0.17353231898006527 1101 68-69 0.09001723906850856 1101 70-71 0.052833544881963235 1101 72-73 0.07189382378140152 1101 74-75 0.15407986622900438 1101 76 0.18389266276694372 1102 1 0.03593719675080109 1102 2 -0.014502941904282807 1102 3 0.0292925484006048 1102 4 0.030957920554264717 1102 5 0.01665218095705967 1102 6 -0.048125791235030135 1102 7 -0.020871439432475825 1102 8 -0.04236649016716143 1102 9 -0.30144379618973716 1102 10-11 -0.24895487234922342 1102 12-13 -0.0010729852042459243 1102 14-15 0.12162786034655682 1102 16-17 0.037852372030585 1102 18-19 0.06823575283520711 1102 20-21 0.11223699348398242 1102 22-23 0.10037324092957789 1102 24-25 0.07452961900214206 1102 26-27 0.07310701004085729 1102 28-29 0.1324353456496823 1102 30-31 0.11737961015636245 1102 32-33 0.12917991044461274 1102 34-35 0.1113531931195979 1102 36-37 0.10114715972858335 1102 38-39 0.07410455094613866 1102 40-41 0.1720477799869542 1102 42-43 0.19357054397467977 1102 44-45 0.16761200892014472 1102 46-47 0.11017724769286019 1102 48-49 0.06707934099581081 1102 50-51 0.19020226783393923 1102 52-53 0.1548010834464506 1102 54-55 0.18721448094105142 1102 56-57 0.15529995695950305 1102 58-59 0.13045486715565602 1102 60-61 0.10492914514115625 1102 62-63 0.13394918356583219 1102 64-65 0.15768176276349521 1102 66-67 0.17874724330144431 1102 68-69 0.06869179901683253 1102 70-71 0.03935489784817747 1102 72-73 0.1546492299208424 1102 74-75 0.1720394064318853 1102 76 0.4770235992438927 1103 1 0.006148294548104616 1103 2 0.028996222284533246 1103 3 0.0157538865860829 1103 4 0.004891706557017983 1103 5 0.012345904236987337 1103 6 0.040809695976321336 1103 7 0.03597333637782185 1103 8 0.034968233719290254 1103 9 0.07202992383570717 1103 10-11 0.14767932342289924 1103 12-13 0.20386939560020778 1103 14-15 0.13942767987051496 1103 16-17 0.054342260934276965 1103 18-19 0.044977272680171154 1103 20-21 0.14320342789868334 1103 22-23 0.10288437275105622 1103 24-25 0.03702425649677821 1103 26-27 0.05881722764762287 1103 28-29 0.004960826795851858 1103 30-31 0.015251989925296527 1103 32-33 0.13648845051177005 1103 34-35 0.11978933862470598 1103 36-37 -0.041933359214006316 1103 38-39 0.03871238538155808 1103 40-41 0.0661815689483305 1103 42-43 0.0809601503298083 1103 44-45 -0.008130975443535249 1103 46-47 0.07367300946448552 1103 48-49 -0.08721214260601329 1103 50-51 0.05032482881856737 1103 52-53 -0.004993809969128904 1103 54-55 0.09052259200778678 1103 56-57 0.10332951360975073 1103 58-59 0.20196258176681425 1103 60-61 0.1353785600733346 1103 62-63 0.10147902868188652 1103 64-65 0.19027544018475595 1103 66-67 0.16534393851883067 1103 68-69 0.07737683873635604 1103 70-71 0.13048494415063772 1103 72-73 0.17090464790039306 1103 74-75 0.23224369939480027 1103 76 0.4239710392947771 2116 1 0.009510604366759878 2116 2 0.047727926709562496 2116 3 0.009402404042376133 2116 4 0.02220867669863935 2116 5 0.008744964539204148 2116 6 0.057423374952435324 2116 7 0.040456910193746864 2116 8 0.03496971589245135 2116 9 0.1018186400514196 2116 10-11 0.22890148635819685 2116 12-13 0.16373362853853735 2116 14-15 0.11404009158857775 2116 16-17 0.061688096398228254 2116 18-19 0.05408726475054948 2116 20-21 0.1416062010446808 2116 22-23 0.13547439624350233 2116 24-25 0.0892916108917916 2116 26-27 0.14961052377840645 2116 28-29 0.08714603174334457 2116 30-31 0.15725418354157483 2116 32-33 0.061377658067890195 2116 34-35 0.17090170958301343 2116 36-37 0.15138315145393477 2116 38-39 0.13098303761186258 2116 40-41 0.2016232936050244 2116 42-43 0.20274976326028593 2116 44-45 0.1308314662885337 2116 46-47 0.1626700969942192 2116 48-49 0.15597690116996432 2116 50-51 0.20150111849449814 2116 52-53 0.1827812257882968 2116 54-55 0.22184850706444337 2116 56-57 0.16682247699266384 2116 58-59 0.2020085291348508 2116 60-61 0.19540564678787575 2116 62-63 0.1969808184059758 2116 64-65 0.21923210144148442 2116 66-67 0.23116335795160126 2116 68-69 -0.022572026289545022 2116 70-71 0.15387623045461396 2116 72-73 0.29740293969582154 2116 74-75 0.18271317774395612 2116 76 0.5120784523838466 1117 1 0.019898900469868863 1117 2 -0.055722596815449776 1117 3 -0.05863703753591665 1117 4 0.017594482840742387 1117 5 0.02284212926139162 1117 6 0.09192890817842425 1117 7 0.10830479052365405 1117 8 0.10227501937902872 1117 9 0.12624215913919556 1117 10-11 0.18320908165181748 1117 12-13 0.2038056493104392 1117 14-15 0.20141746054343912 1117 16-17 0.08302858934718671 1117 18-19 0.02104568705762233 1117 20-21 0.1510603241184043 1117 22-23 0.08026639231319876 1117 24-25 -0.07192047679804148 1117 26-27 0.1128855357812455 1117 28-29 0.11794265910326374 1117 30-31 0.016215156522243035 1117 32-33 0.14652035685785592 1117 34-35 0.18854834444820057 1117 36-37 0.18423283267561175 1117 38-39 0.04242145661140029 1117 40-41 0.1546056411410035 1117 42-43 -0.14258264932755793 1117 44-45 -0.015220260090636373 1117 46-47 -0.12938648043238743 1117 48-49 0.03322020332384312 1117 50-51 -0.15002295367377627 1117 52-53 0.06144376846868482 1117 54-55 -0.03962458887895082 1117 56-57 0.07029266692621405 1117 58-59 0.10748892027289259 1117 60-61 0.1841911194204684 1117 62-63 0.1642619858457124 1117 64-65 0.1694322294966284 1117 66-67 0.14319193681893694 1117 68-69 0.22318518817226618 1117 70-71 0.15471251840510547 1117 72-73 0.08093506019280738 1117 74-75 0.27616256896059355 1117 76 0.4850914467398155 2117 1 -0.10237942634383757 2117 2 -0.0184981867029137 2117 3 -0.00949013340533611 2117 4 0.004787876096699506 2117 5 0.01665693022184911 2117 6 -0.014180977691907515 2117 7 0.010407552417341037 2117 8 -0.06152943966837654 2117 9 -0.18994920255983772 2117 10-11 -0.30311400388679743 2117 12-13 -0.8099554460951595 2117 14-15 -1.1801064932376946 2117 16-17 -0.0759532398194196 2117 18-19 0.06075731190010458 2117 20-21 0.15678259273359885 2117 22-23 0.15757334897534747 2117 24-25 0.0865736617544286 2117 26-27 0.1718090508147725 2117 28-29 0.13942847108889111 2117 30-31 0.12535605602921862 2117 32-33 0.13803760466171866 2117 34-35 0.17477979137706967 2117 36-37 0.2411132989547653 2117 38-39 0.10445394842027156 2117 40-41 0.24585473127804391 2117 42-43 0.23919804800599564 2117 44-45 0.2128181270120919 2117 46-47 0.06185080697741796 2117 48-49 0.1787589704344228 2117 50-51 0.24503512710654007 2117 52-53 0.2975145424918324 2117 54-55 0.24525153559544322 2117 56-57 0.12223936005113245 2117 58-59 0.1814457196264243 2117 60-61 0.22540654643251656 2117 62-63 0.2046854568919585 2117 64-65 0.1647555514969028 2117 66-67 0.24560674372331448 2117 68-69 0.2390969780900889 2117 70-71 0.2270306299213729 2117 72-73 0.12738911788271423 2117 74-75 0.19972116413479313 2117 76 0.41103527353040903 1116 1 -0.0019354322257143508 1116 2 -0.02987464200909784 1116 3 0.02175679515028861 1116 4 0.02135353495026493 1116 5 0.0046256746891160105 1116 6 -0.00784249090772704 1116 7 0.03877700756053315 1116 8 -0.1248406571926921 1116 9 -0.22782105622875548 1116 10-11 -0.2429919223265884 1116 12-13 -0.0030312609524543177 1116 14-15 0.1424859420473794 1116 16-17 0.022167607077605567 1116 18-19 0.0574158010405057 1116 20-21 0.18529182904598684 1116 22-23 0.13090261284980187 1116 24-25 0.07866169458533534 1116 26-27 0.1119995142863246 1116 28-29 0.11332974752647829 1116 30-31 0.07393934639373612 1116 32-33 0.10587341159287433 1116 34-35 0.11736631698713751 1116 36-37 0.06818669236023567 1116 38-39 0.033480799752275914 1116 40-41 0.17599738675844634 1116 42-43 0.1478280818700668 1116 44-45 0.1944687920472532 1116 46-47 0.0783666976309263 1116 48-49 0.1403963842956344 1116 50-51 0.18078945509225974 1116 52-53 0.16759245923284993 1116 54-55 0.13548840465021783 1116 56-57 0.1217270252040592 1116 58-59 0.11757320799505777 1116 60-61 0.14439948883477172 1116 62-63 0.014921883971283023 1116 64-65 0.13954292986954187 1116 66-67 0.13076385514683864 1116 68-69 0.17940916865901357 1116 70-71 0.15844732926125005 1116 72-73 0.06847697168047517 1116 74-75 0.16547503506935612 1116 76 0.26192930491676947 2118 1 -0.16590124303185405 2118 2 0.031014560968969818 2118 3 0.00691827445965032 2118 4 -0.05298143940725453 2118 5 -0.0011633200070875205 2118 6 0.06438440715594851 2118 7 0.06564654588184737 2118 8 0.014020173804524916 2118 9 0.09693589525453206 2118 10-11 0.17431722883651446 2118 12-13 0.21840140484714254 2118 14-15 0.1920587598852208 2118 16-17 0.10184821595574078 2118 18-19 0.07994715718130863 2118 20-21 0.1540922596896479 2118 22-23 0.11050811787759329 2118 24-25 0.05981328549520981 2118 26-27 0.08212017463291943 2118 28-29 0.08738128864491301 2118 30-31 0.12769326323845576 2118 32-33 0.12855004453962238 2118 34-35 0.1486715953692226 2118 36-37 0.16673974341440356 2118 38-39 0.0561577433929088 2118 40-41 0.11299367429548113 2118 42-43 0.1465958699174692 2118 44-45 0.19534207945685722 2118 46-47 0.08352547719317727 2118 48-49 0.1480609831543731 2118 50-51 0.19101592607977835 2118 52-53 0.26912098908274373 2118 54-55 0.16810620021641398 2118 56-57 0.15029695461221593 2118 58-59 0.14403241107328313 2118 60-61 0.12359116788228164 2118 62-63 0.17458403617050067 2118 64-65 0.12276346791971093 2118 66-67 0.19581772690804655 2118 68-69 0.2141070597931929 2118 70-71 0.16015991051409628 2118 72-73 0.188715322871154 2118 74-75 0.24122352040010497 2118 76 0.3807948277571924 1119 1 -0.0951042842906844 1119 2 0.01096905562653916 1119 3 -0.029584266358561706 1119 4 -0.018948466116825102 1119 5 -0.00938178772396725 1119 6 0.07047414354137516 1119 7 0.07572776157849148 1119 8 0.007658114269212035 1119 9 0.08148451026798398 1119 10-11 0.20104221588315596 1119 12-13 0.22807060570404758 1119 14-15 0.1386408089771507 1119 16-17 0.016234608501065395 1119 18-19 0.0654701559519566 1119 20-21 0.13866965594605318 1119 22-23 0.07175995569864568 1119 24-25 0.03828116951839888 1119 26-27 0.11816974407417291 1119 28-29 0.051026472329041894 1119 30-31 0.11670480801097227 1119 32-33 0.11899265466912112 1119 34-35 0.167398386664793 1119 36-37 0.055100976182394845 1119 38-39 0.00833041384846922 1119 40-41 0.10541372511760017 1119 42-43 0.13124750665164697 1119 44-45 0.13635767766580642 1119 46-47 0.027746707909386714 1119 48-49 0.04995174151526527 1119 50-51 0.16283674782135904 1119 52-53 0.1910471307219055 1119 54-55 0.11643428736674366 1119 56-57 0.13075513903233826 1119 58-59 0.11924405226837109 1119 60-61 0.1479782462784982 1119 62-63 0.1461226288275128 1119 64-65 0.14141684723387016 1119 66-67 0.14780978515810972 1119 68-69 -7.008644934813901E-4 1119 70-71 0.08455972614124363 1119 72-73 0.15776860656962555 1119 74-75 0.1749288990272646 1119 76 0.1756856420235806 2119 1 0.04368341345704607 2119 2 0.014562686384678614 2119 3 0.04087376807994758 2119 4 0.029293315186308178 2119 5 0.04614925576574791 2119 6 0.07874994792523182 2119 7 0.04941291008089621 2119 8 0.07079906490974253 2119 9 0.13627522746412524 2119 10-11 0.18390703185195179 2119 12-13 0.2558891456544643 2119 14-15 0.17626221691783428 2119 16-17 0.0409620745672683 2119 18-19 0.07566368722373795 2119 20-21 0.14127579807212953 2119 22-23 0.09325400765956005 2119 24-25 -0.04262571929830017 2119 26-27 0.07861385913550123 2119 28-29 -0.01246085558769039 2119 30-31 0.12761823687065288 2119 32-33 0.07829388722007735 2119 34-35 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-0.07478512179191199 2104 42-43 0.018925347526042913 2104 44-45 0.021502064023785294 2104 46-47 0.0010041277485441924 2104 48-49 -0.05050643885412143 2104 50-51 -0.0983725839393017 2104 52-53 -0.10700321620819153 2104 54-55 -0.1387272355117446 2104 56-57 -0.07258845797782243 2104 58-59 0.04799198661620352 2104 60-61 -0.027421473475257585 2104 62-63 -0.06915067066518077 2104 64-65 -0.17170191591951323 2104 66-67 -0.17520176301520962 2104 68-69 -0.0351975664955404 2104 70-71 -0.09833526136607418 2104 72-73 -0.14252486040218315 2104 74-75 -0.06660627874246217 2104 76 -0.3993122097810584 2111 1 0.11468941502397456 2111 2 -0.004882519709013877 2111 3 0.02342078893602917 2111 4 0.014596435031101862 2111 5 -0.020702523463512534 2111 6 -0.11987541238585209 2111 7 -0.034469059916318656 2111 8 -0.04744958477120775 2111 9 -0.03089234030893806 2111 10-11 -0.3613346757469955 2111 12-13 -0.47506343883542 2111 14-15 -0.29600558108927544 2111 16-17 -0.0367867141354381 2111 18-19 -0.061207484242764565 2111 20-21 0.12414692231007507 2111 22-23 -0.05989810826392983 2111 24-25 0.028480737474218643 2111 26-27 -0.03831864689378506 2111 28-29 0.023593832466843878 2111 30-31 -0.07062004865167637 2111 32-33 -0.029730261489511633 2111 34-35 -0.02449492461474989 2111 36-37 0.07564642374880748 2111 38-39 0.07724467294853099 2111 40-41 -0.06407432469802643 2111 42-43 -0.002394300642855285 2111 44-45 0.028180675027734026 2111 46-47 -0.03429631833759572 2111 48-49 0.02984058579298221 2111 50-51 0.012741114324875014 2111 52-53 -0.13560135141706553 2111 54-55 -0.007889309372316688 2111 56-57 -0.07475162818190029 2111 58-59 -0.20346157514401852 2111 60-61 0.005841448298973262 2111 62-63 -0.05368728445985482 2111 64-65 -0.02513892439851162 2111 66-67 -0.03296795389279339 2111 68-69 -0.09788329929479289 2111 70-71 -0.0013713015606526824 2111 72-73 -0.04294904492294194 2111 74-75 0.038139848580236446 2111 76 -0.11331061474302118 2110 1 0.016148356444993794 2110 2 -0.026957330287586956 2110 3 -0.01798208603073448 2110 4 -0.011900920285185634 2110 5 0.03378017902205244 2110 6 -0.14551356673266014 2110 7 -0.013393767899870568 2110 8 -0.06013386520432107 2110 9 -0.07153744164687481 2110 10-11 -0.3124855389493604 2110 12-13 -0.4318686319934244 2110 14-15 -0.1495452340494765 2110 16-17 -0.05884843101108572 2110 18-19 -0.09618276792419067 2110 20-21 -0.0476799335977276 2110 22-23 -0.05096282858097112 2110 24-25 -0.05986140137433438 2110 26-27 -0.03579727850802783 2110 28-29 -0.09776588604589875 2110 30-31 -0.06032194900722487 2110 32-33 -0.12472927799607447 2110 34-35 -0.007719497156649879 2110 36-37 0.013127949657679494 2110 38-39 0.043846887328214734 2110 40-41 -0.18734933926477026 2110 42-43 -0.041522193581705835 2110 44-45 -0.15072826188377775 2110 46-47 -0.014310637376610202 2110 48-49 -0.13455862283924347 2110 50-51 0.04032275527267615 2110 52-53 -0.17410787975168773 2110 54-55 -0.062303465345877385 2110 56-57 -0.10277630531708581 2110 58-59 -0.08015509660454967 2110 60-61 -0.2004295657506674 2110 62-63 -0.25508436302021664 2110 64-65 -0.17059257088241253 2110 66-67 -0.30648167936689674 2110 68-69 -0.30547648419830153 2110 70-71 -0.22592850233021977 2110 72-73 -0.2612876162260207 2110 74-75 -0.16075916643130483 2110 76 -0.2635496187929789 2109 1 0.034208775174079165 2109 2 0.0033606685004130554 2109 3 -0.008741756447861349 2109 4 -9.87546869879452E-5 2109 5 0.007337936244582011 2109 6 -0.20557640601813887 2109 7 -0.1045471053458158 2109 8 -0.10120091606858495 2109 9 -0.20519033666194275 2109 10-11 -0.4742339531276727 2109 12-13 -0.5250556277709535 2109 14-15 -0.14266383336991595 2109 16-17 -0.0711661959323493 2109 18-19 -0.09285828774682159 2109 20-21 0.053424410809199685 2109 22-23 -0.021549304697138894 2109 24-25 -0.060616864848512364 2109 26-27 -0.11849982164183359 2109 28-29 -0.18323137308277637 2109 30-31 -0.08521387641309985 2109 32-33 -0.11646865034301612 2109 34-35 -0.15574273159326424 2109 36-37 -0.15451597134541828 2109 38-39 -0.060400669463000156 2109 40-41 -0.3182760366959059 2109 42-43 -0.20714271669432804 2109 44-45 -0.227150863940075 2109 46-47 -0.10488554853301935 2109 48-49 -0.21021590621094077 2109 50-51 -0.16027047328355337 2109 52-53 -0.472978741710115 2109 54-55 -0.29800631869188976 2109 56-57 -0.10516599933451687 2109 58-59 -0.017502119992229836 2109 60-61 -0.17633461365103642 2109 62-63 0.0015244389729502927 2109 64-65 -0.0916381685096681 2109 66-67 -0.1628709370967556 2109 68-69 -0.024757185456174113 2109 70-71 -0.16343322529392879 2109 72-73 -0.24720366143900918 2109 74-75 -0.2514746440101021 2109 76 -0.5353547986591636 2108 1 0.04182303428542866 2108 2 0.016900755142025048 2108 3 -0.008553536446029852 2108 4 0.02324991520038111 2108 5 -0.02402080303532017 2108 6 0.033546091518473986 2108 7 -0.0072137260928855085 2108 8 0.008681546728908529 2108 9 0.014476194489390082 2108 10-11 0.13628277135051547 2108 12-13 0.18196442673699664 2108 14-15 0.07739832749223297 2108 16-17 0.012928665635328684 2108 18-19 0.013244048975110445 2108 20-21 0.09478097109703754 2108 22-23 -0.03992532886363165 2108 24-25 -0.022808299200164583 2108 26-27 -0.006250130359731543 2108 28-29 -0.00771577815442015 2108 30-31 -0.10065612430820181 2108 32-33 -0.06910978275963942 2108 34-35 -0.04330834963956676 2108 36-37 6.53707853565777E-4 2108 38-39 0.004456666637281614 2108 40-41 -0.07279197887752531 2108 42-43 0.005240114056356049 2108 44-45 3.585120129443453E-4 2108 46-47 0.07921818724825869 2108 48-49 -0.021926729504535558 2108 50-51 0.011039686653539604 2108 52-53 -0.08311029314425156 2108 54-55 -0.018958773623403147 2108 56-57 -0.056523867280240836 2108 58-59 -0.06410631827991864 2108 60-61 -0.10454345689188216 2108 62-63 -0.0519608370060638 2108 64-65 -0.10050939447783946 2108 66-67 -0.1037961377194847 2108 68-69 -0.2138621627409094 2108 70-71 -0.04115170010979341 2108 72-73 0.033139065745707796 2108 74-75 -0.07186844043905438 2108 76 -0.05144363626752124 >>END_MODULE >>Per sequence quality scores pass #Quality Count 14 2.0 15 9.0 16 19.0 17 18.0 18 21.0 19 29.0 20 47.0 21 140.0 22 256.0 23 417.0 24 649.0 25 870.0 26 1088.0 27 1538.0 28 2321.0 29 3657.0 30 6123.0 31 11353.0 32 20276.0 33 30304.0 34 53421.0 35 93842.0 36 188315.0 37 1629734.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 48.32138145779132 2.6373365146305927 44.06253225196618 4.978749775611913 2 3.70539935209927 2.41585874727127 4.491576948116583 89.38716495251288 3 3.81129585526467 41.81067857403144 4.742206824430446 49.63581874627344 4 3.995453053610044 2.0244574455024313 89.17385564521297 4.80623385567456 5 3.5457964468666128 2.53730956360369 43.99332044966639 49.923573539863305 6 43.34082190360336 2.4688803682557015 5.178167809517381 49.01212991862355 7 3.737535150057546 2.9066511319186734 45.07791585899184 48.277897859031945 8 4.774538274126671 3.118884354659862 88.99297561347825 3.1136017577352137 9 5.478395401401551 3.05187363441201 88.19158609483533 3.2781448693511064 10-11 66.74157682583424 25.793062091546425 3.2022564514937764 4.26310463112555 12-13 26.033664816290354 23.42088259477248 26.22934101070753 24.316111578229634 14-15 3.9722683226629765 45.52133606658811 23.758479668605087 26.747915942143823 16-17 23.476741165957186 3.541736673304152 45.98781872279524 26.993703437943427 18-19 26.76797024528369 45.151334173657546 4.25728399192154 23.82341158913722 20-21 24.006058357547282 2.3417562242335768 49.53220880839014 24.11997660982901 22-23 26.07585222228581 2.979066731427392 42.79588290047832 28.14919814580848 24-25 4.369465807168583 23.489972114736048 25.8017685938852 46.33879348421017 26-27 13.100277874380822 36.560633207284695 22.20331737304281 28.135771545291664 28-29 23.509170441522386 3.8328420029064065 26.073822335504577 46.58416522006663 30-31 48.287435881257004 4.317569183677363 22.631452288611747 24.763542646453885 32-33 57.78640112812792 11.670601712246183 3.983420471726123 26.55957668789977 34-35 4.26787364223808 45.399420577378066 23.880052767273725 26.452653013110133 36-37 17.93307145348209 17.575444532976856 32.43773750286752 32.053746510673534 38-39 46.99955831620158 3.8000947932670366 46.25977463854564 2.940572251985743 40-41 27.257319698363712 44.00276064602247 24.437904785103466 4.302014870510343 42-43 38.20762953734722 3.172321735587437 54.26202854656682 4.35802018049851 44-45 3.9581080281288505 5.001054073738205 86.4436090115234 4.597228886609546 46-47 5.539022983698786 85.8672189915229 4.547337693432314 4.046420331346001 48-49 16.575688608519947 27.184341600108393 30.422915905459124 25.81705388591254 50-51 42.6163968873765 25.08688159988339 27.062230459160393 5.234491053579718 52-53 45.430627029581075 27.80969346753086 22.552017683004074 4.207661819883988 54-55 6.199812272157438 66.3822134961547 3.7007526233229586 23.717221608364895 56-57 25.216451963340734 23.490950373425797 5.458928053475533 45.83366960975793 58-59 2.9103440584724782 24.06714474168835 49.38817255896332 23.634338640875853 60-61 4.537555106534817 44.96744599645186 24.759018200013795 25.735980696999533 62-63 3.79508121748207 25.26847086916817 58.611904723473174 12.324543189876588 64-65 28.186029585477556 41.98250971288597 26.124129288624953 3.7073314130115254 66-67 26.159273232054208 26.638619990031543 23.30349155200252 23.898615225911723 68-69 4.398128786778247 23.82282463392337 45.34263266043809 26.43641391886029 70-71 48.79556790118022 5.486270383854036 22.77435142671693 22.943810288248816 72-73 28.785922270499288 63.66590704879408 3.6657798751644086 3.8823908055422267 74-75 21.866380623825783 25.48063072250763 25.60958967428388 27.04339897938271 76 16.06188268819618 75.56515227330199 3.4552096921957944 4.917755346306022 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.5 7 0.5 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 1.5 17 3.0 18 2.0 19 3.5 20 4.5 21 3.0 22 3.5 23 6.5 24 14.5 25 20.0 26 16.5 27 76.5 28 293.5 29 447.0 30 1281.5 31 2600.5 32 11498.5 33 19912.0 34 125375.0 35 141936.0 36 269683.5 37 486333.0 38 265538.5 39 28561.0 40 10298.0 41 27981.5 42 47745.0 43 28124.5 44 9465.5 45 9976.5 46 9526.0 47 22084.5 48 294449.5 49 317831.0 50 81406.0 51 162128.5 52 135618.0 53 36555.0 54 44725.0 55 26761.0 56 6353.0 57 3676.5 58 3444.0 59 3811.0 60 4654.5 61 5636.5 62 6142.0 63 6781.0 64 7870.0 65 8808.0 66 9559.5 67 9823.0 68 9852.0 69 9722.5 70 9028.5 71 8493.0 72 7980.0 73 6660.5 74 5162.5 75 4471.0 76 3845.0 77 2668.0 78 1752.0 79 1387.0 80 1105.0 81 646.5 82 360.0 83 250.0 84 177.0 85 83.0 86 40.5 87 19.0 88 18.5 89 12.0 90 5.0 91 3.5 92 3.0 93 2.0 94 1.5 95 1.5 96 1.0 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 2.4456467243741467E-5 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 2044449.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 7.83487740858931 #Duplication Level Percentage of deduplicated Percentage of total 1 90.92612893381147 7.1239507343399815 2 3.666699313289741 0.5745627922756745 3 1.7959677296354053 0.4221356097442761 4 0.9506222275703987 0.29792034459576655 5 0.682138040260663 0.26722339605888273 6 0.33471514542695185 0.15734712787309862 7 0.2238109785508135 0.12274721057494246 8 0.165294242157376 0.1036048098918973 9 0.0857599785794321 0.06047270268597862 >10 0.7264384416304264 1.2114105845346834 >50 0.17345911534205416 0.9699704654955084 >100 0.19351826970127795 3.323862010499204 >500 0.03865909266236389 2.2015163287023487 >1k 0.028682552602711926 4.160525841767422 >5k 0.003741202513397208 1.874151670514814 >10k+ 0.004364736265630076 77.12859837044552 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GCCTCCCTTAGGTCACTAGTTGCCTCATCCGGCACACTGGAGTTTAACAA 568482 27.806122823313274 No Hit TCATTGTTTGGCATAGTCACGTTCAATGCTGGGTATGTGTAGTTTAAGTG 449984 22.01003791241552 No Hit GCCTCCCTTAGGTCACTAGTTGCCTCATCCGGCACCCTGGAGTTTAACAA 242330 11.85307141435174 No Hit TCATTGTTTGGCATAGTCACGTTCAGTGCTGGATATTTGTATTTTAATTG 225659 11.037643883510912 No Hit TCATTGTTTGGCATAGTCACGTTCAGCACTGGATATGTGCTTCCTGATTT 43759 2.1403811002377657 No Hit GCCTCCCTTAGGTCACTAGTTGCCTCGTCAGGCACTCTGGAGTTTATCAC 37897 1.853653478272141 No Hit ATTGTTTGGCATAGTCACGTTCAATGCTGGGTATGTGTAGTTTAAGTGGG 10675 0.5221455756538804 No Hit TGGCATAGTCACGTTCAATGCTGGGTATGTGTAGTTTAAGTGGGTCAACC 7968 0.38973826199626405 No Hit CTCCCTTAGGTCACTAGTTGCCTCATCCGGCACACTGGAGTTTAACAATG 7409 0.36239593161776107 No Hit TCATTGTTTGGCATAGTCACTCAAAACGGAACAAGTTCTGCTTGCATAAG 6640 0.3247818849968867 No Hit CATTGTTTGGCATAGTCACGTTCAATGCTGGGTATGTGTAGTTTAAGTGG 5927 0.28990696270731137 No Hit CCTCCCTTAGGTCACTAGTTGCCTCATCCGGCACACTGGAGTTTAACAAT 5339 0.2611461572286714 No Hit TGTTTGGCATAGTCACGTTCAATGCTGGGTATGTGTAGTTTAAGTGGGTC 5080 0.24847770719641332 No Hit CTCCCTTAGGTCACTAGTTGCCTCATCCGGCACCCTGGAGTTTAACAATG 4595 0.22475493396998408 No Hit TTGGCATAGTCACGTTCAATGCTGGGTATGTGTAGTTTAAGTGGGTCAAC 4128 0.20191259356432956 No Hit TTGTTTGGCATAGTCACGTTCAATGCTGGGTATGTGTAGTTTAAGTGGGT 3791 0.18542893464204782 No Hit GCCTCCCTTAGGTCACTAGTTGCCTCATCCGGCACACTGGAGTTTAACAC 3486 0.1705104896233655 No Hit TCCCTTAGGTCACTAGTTGCCTCATCCGGCACACTGGAGTTTAACAATGA 3354 0.16405398227101775 No Hit TCATTGTTTGGACTGGAGTCACTCAAAACGGAACAAGTTCTGCTTGCATA 3084 0.15084748995939737 No Hit CATTGTTTGGCATAGTCACGTTCAGTGCTGGATATTTGTATTTTAATTGG 2970 0.14527141542782432 No Hit CCCTTAGGTCACTAGTTGCCTCATCCGGCACACTGGAGTTTAACAATGAA 2831 0.13847251753406417 No Hit CCTCCCTTAGGTCACTAGTTGCCTCATCCGGCACCCTGGAGTTTAACAAT 2788 0.13636926135110242 No Hit ATTGTTTGGCATAGTCACGTTCAGTGCTGGATATTTGTATTTTAATTGGT 2438 0.11924973428048341 No Hit TTTGGCATAGTCACGTTCAATGCTGGGTATGTGTAGTTTAAGTGGGTCAA 2234 0.10927149564503688 No Hit TTGGCATAGTCACGTTCAGTGCTGGATATTTGTATTTTAATTGGTGCAAC 2197 0.107461717069 No Hit TCATTGTTTGGCATAGTCACGTTCAGTGCTGGGTATGTGTAGTTTAAGTG 2172 0.10623889370681293 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 9.782586897496587E-4 0.0 0.0 2 9.782586897496587E-4 0.0 0.0 3 9.782586897496587E-4 0.0 0.0 4 9.782586897496587E-4 0.0 0.0 5 9.782586897496587E-4 0.0 0.0 6 9.782586897496587E-4 0.0 0.0 7 9.782586897496587E-4 0.0 0.0 8 9.782586897496587E-4 0.0 0.0 9 9.782586897496587E-4 0.0 0.0 10 9.782586897496587E-4 0.0 0.0 11 9.782586897496587E-4 0.0 0.0 12 9.782586897496587E-4 0.0 0.0 13 9.782586897496587E-4 0.0 0.0 14 9.782586897496587E-4 0.0 0.0 15 9.782586897496587E-4 0.0 0.0 16 9.782586897496587E-4 0.0 0.0 17 9.782586897496587E-4 0.0 0.0 18 9.782586897496587E-4 0.0 0.0 19 0.0010271716242371417 0.0 0.0 20 0.0010271716242371417 0.0 0.0 21 0.0010271716242371417 0.0 0.0 22 0.0010760845587246247 0.0 0.0 23 0.0010760845587246247 0.0 0.0 24 0.0010760845587246247 0.0 0.0 25 0.0010760845587246247 0.0 0.0 26 0.0010760845587246247 0.0 0.0 27 0.0010760845587246247 0.0 0.0 28 0.0010760845587246247 0.0 0.0 29 0.0011249974932121074 0.0 0.0 30 0.0011249974932121074 0.0 0.0 31 0.0012717362966745563 0.0 0.0 32 0.0013695621656495223 0.0 0.0 33 0.0014184751001370052 0.0 0.0 34 0.001516300969111971 0.0 0.0 35 0.0016141268380869369 0.0 0.0 36 0.0017608656415493856 0.0 0.0 37 0.0018586915105243515 0.0 0.0 38 0.0021521691174492493 0.0 0.0 39 0.0023478208553991808 0.0 0.0 40 0.0026412984623240786 0.0 0.0 41 0.0026902113968115616 0.0 0.0 42 0.002983689003736459 0.0 0.0 43 0.0034728183486112886 0.0 0.0 44 0.003521731283098771 0.0 0.0 45 0.00366847008656122 0.0 0.0 46 0.004108686496948567 0.0 0.0 47 0.004548902907335913 0.0 0.0 48 0.004891293448748293 0.0 0.0 49 0.005233683990160674 0.0 0.0 50 0.005722813335035504 0.0 0.0 51 0.00616302974542285 0.0 0.0 52 0.0066032461558101965 0.0 0.0 53 0.00699454963171006 0.0 0.0 54 0.007972808321459718 0.0 0.0 55 0.008364111797359582 0.0 0.0 56 0.009048892880184343 0.0 0.0 57 0.009635848094034139 0.0 0.0 58 0.01100541025968366 0.0 0.0 59 0.01183693014597087 0.0 0.0 60 0.01281518883572053 0.0 0.0 61 0.01384236045995767 0.0 0.0 62 0.014967357953169778 0.0 0.0 63 0.015945616642919436 0.0 0.0 64 0.01682604946369413 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATCCTAA 20 7.930092E-5 70.0 41 TTAGGTT 15 0.002225153 70.0 8 TTAGGTA 40 1.3460522E-10 70.0 8 TAGAGTT 50 0.0 70.0 38 TTAGGCA 15 0.002225153 70.0 8 TTAGGAC 25 2.8417853E-6 70.0 8 ACAGTTG 50 0.0 70.0 15 CCCCTAG 30 1.0246913E-7 70.0 5 ATTAGTA 20 7.930092E-5 70.0 35 TTAATTA 25 2.8417853E-6 70.0 44 ATAGGAC 15 0.002225153 70.0 62 TAGGTAT 30 1.0246913E-7 70.0 30 TTAGACT 70 0.0 70.0 63 GGACTCT 25 2.8417853E-6 70.0 65 TTGTCTG 20 7.930092E-5 70.0 4 GAATCTA 25 2.8417853E-6 70.0 63 CATCTTA 20 7.930092E-5 70.0 40 CTCTTTG 30 1.0246913E-7 70.0 16 ATTGTCA 15 0.002225153 70.0 13 CTAAGTT 60 0.0 70.0 44 >>END_MODULE