FastQCFastQC Report
Tue 25 Aug 2015
000000000-AHV5L l01n01 kokuya1.34100000033cdf.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AHV5L l01n01 kokuya1.34100000033cdf.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2044449
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTCCCTTAGGTCACTAGTTGCCTCATCCGGCACACTGGAGTTTAACAA56848227.806122823313274No Hit
TCATTGTTTGGCATAGTCACGTTCAATGCTGGGTATGTGTAGTTTAAGTG44998422.01003791241552No Hit
GCCTCCCTTAGGTCACTAGTTGCCTCATCCGGCACCCTGGAGTTTAACAA24233011.85307141435174No Hit
TCATTGTTTGGCATAGTCACGTTCAGTGCTGGATATTTGTATTTTAATTG22565911.037643883510912No Hit
TCATTGTTTGGCATAGTCACGTTCAGCACTGGATATGTGCTTCCTGATTT437592.1403811002377657No Hit
GCCTCCCTTAGGTCACTAGTTGCCTCGTCAGGCACTCTGGAGTTTATCAC378971.853653478272141No Hit
ATTGTTTGGCATAGTCACGTTCAATGCTGGGTATGTGTAGTTTAAGTGGG106750.5221455756538804No Hit
TGGCATAGTCACGTTCAATGCTGGGTATGTGTAGTTTAAGTGGGTCAACC79680.38973826199626405No Hit
CTCCCTTAGGTCACTAGTTGCCTCATCCGGCACACTGGAGTTTAACAATG74090.36239593161776107No Hit
TCATTGTTTGGCATAGTCACTCAAAACGGAACAAGTTCTGCTTGCATAAG66400.3247818849968867No Hit
CATTGTTTGGCATAGTCACGTTCAATGCTGGGTATGTGTAGTTTAAGTGG59270.28990696270731137No Hit
CCTCCCTTAGGTCACTAGTTGCCTCATCCGGCACACTGGAGTTTAACAAT53390.2611461572286714No Hit
TGTTTGGCATAGTCACGTTCAATGCTGGGTATGTGTAGTTTAAGTGGGTC50800.24847770719641332No Hit
CTCCCTTAGGTCACTAGTTGCCTCATCCGGCACCCTGGAGTTTAACAATG45950.22475493396998408No Hit
TTGGCATAGTCACGTTCAATGCTGGGTATGTGTAGTTTAAGTGGGTCAAC41280.20191259356432956No Hit
TTGTTTGGCATAGTCACGTTCAATGCTGGGTATGTGTAGTTTAAGTGGGT37910.18542893464204782No Hit
GCCTCCCTTAGGTCACTAGTTGCCTCATCCGGCACACTGGAGTTTAACAC34860.1705104896233655No Hit
TCCCTTAGGTCACTAGTTGCCTCATCCGGCACACTGGAGTTTAACAATGA33540.16405398227101775No Hit
TCATTGTTTGGACTGGAGTCACTCAAAACGGAACAAGTTCTGCTTGCATA30840.15084748995939737No Hit
CATTGTTTGGCATAGTCACGTTCAGTGCTGGATATTTGTATTTTAATTGG29700.14527141542782432No Hit
CCCTTAGGTCACTAGTTGCCTCATCCGGCACACTGGAGTTTAACAATGAA28310.13847251753406417No Hit
CCTCCCTTAGGTCACTAGTTGCCTCATCCGGCACCCTGGAGTTTAACAAT27880.13636926135110242No Hit
ATTGTTTGGCATAGTCACGTTCAGTGCTGGATATTTGTATTTTAATTGGT24380.11924973428048341No Hit
TTTGGCATAGTCACGTTCAATGCTGGGTATGTGTAGTTTAAGTGGGTCAA22340.10927149564503688No Hit
TTGGCATAGTCACGTTCAGTGCTGGATATTTGTATTTTAATTGGTGCAAC21970.107461717069No Hit
TCATTGTTTGGCATAGTCACGTTCAGTGCTGGGTATGTGTAGTTTAAGTG21720.10623889370681293No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCCTAA207.930092E-570.041
TTAGGTT150.00222515370.08
TTAGGTA401.3460522E-1070.08
TAGAGTT500.070.038
TTAGGCA150.00222515370.08
TTAGGAC252.8417853E-670.08
ACAGTTG500.070.015
CCCCTAG301.0246913E-770.05
ATTAGTA207.930092E-570.035
TTAATTA252.8417853E-670.044
ATAGGAC150.00222515370.062
TAGGTAT301.0246913E-770.030
TTAGACT700.070.063
GGACTCT252.8417853E-670.065
TTGTCTG207.930092E-570.04
GAATCTA252.8417853E-670.063
CATCTTA207.930092E-570.040
CTCTTTG301.0246913E-770.016
ATTGTCA150.00222515370.013
CTAAGTT600.070.044
Produced by FastQC (version 0.11.2)