##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename 000000000-AHJKW l01n03.33300000033adc.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 20316831 Sequences flagged as poor quality 0 Sequence length 251 %GC 58 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.14611461797364 32.0 32.0 33.0 18.0 33.0 2 31.441360761429774 32.0 32.0 33.0 29.0 33.0 3 29.92518980937529 32.0 32.0 33.0 16.0 33.0 4 30.794839756259233 32.0 32.0 33.0 29.0 33.0 5 30.674778315574905 32.0 32.0 33.0 29.0 33.0 6 34.896398951194705 37.0 35.0 37.0 32.0 37.0 7 34.15260367131075 37.0 34.0 37.0 20.0 37.0 8 33.09782844578468 33.0 32.0 37.0 29.0 37.0 9 34.686300584968194 37.0 35.0 37.0 32.0 37.0 10-14 35.21399273341399 37.4 34.4 37.4 32.2 37.4 15-19 34.4695381282642 37.2 34.4 38.0 25.4 38.0 20-24 34.42673291912504 37.2 34.0 38.0 23.2 38.0 25-29 34.500388156007205 37.2 34.4 38.4 22.6 38.4 30-34 34.623741556938676 37.0 34.6 38.0 29.0 38.6 35-39 33.95187673707578 36.4 33.8 38.2 20.0 38.4 40-44 33.136819073801426 37.0 32.8 38.2 16.6 39.0 45-49 34.65818372953932 37.4 34.6 38.4 25.6 39.0 50-54 32.99365579208687 37.0 32.6 38.0 16.0 39.0 55-59 34.66180501279948 36.8 35.2 38.0 25.4 38.4 60-64 34.893596525954266 37.2 34.6 38.2 28.0 38.4 65-69 32.20593576823078 35.2 28.6 37.4 17.8 38.4 70-74 34.10572558289233 36.8 33.8 38.0 22.8 38.4 75-79 33.81574609741057 37.2 33.4 38.4 19.6 39.0 80-84 32.377965490779545 35.8 27.6 37.6 21.6 38.4 85-89 34.31402285129999 37.0 34.8 38.2 22.4 38.6 90-94 32.37935485115765 36.4 30.6 37.4 16.2 38.4 95-99 33.73274423555524 36.6 33.6 38.2 19.6 38.6 100-104 31.72827441444977 35.2 28.4 37.2 19.6 37.8 105-109 28.90007347110383 30.8 23.0 36.4 14.2 38.2 110-114 30.301736506052542 33.8 26.8 36.6 15.8 38.2 115-119 29.818810699365468 34.0 19.0 37.4 14.8 38.6 120-124 33.50343535367303 36.8 32.4 38.2 20.6 39.0 125-129 30.615986134845535 35.0 23.4 38.0 15.0 38.6 130-134 30.744896268517465 34.2 24.4 37.8 17.0 38.6 135-139 27.657400477466194 30.4 16.0 37.2 14.4 38.2 140-144 27.010013352968286 29.4 15.6 35.8 13.2 38.0 145-149 26.654219735351443 28.6 21.8 33.0 14.6 35.6 150-154 25.96950870930609 28.0 15.4 34.6 12.8 38.0 155-159 25.461868162411747 27.0 14.6 34.6 12.8 37.4 160-164 24.87631712839468 26.0 14.6 34.0 12.8 36.8 165-169 22.631210389061167 21.2 14.2 28.4 12.6 35.2 170-174 24.680780009441435 25.8 13.8 33.4 12.8 36.8 175-179 21.582377802916213 18.8 12.6 28.8 12.2 34.4 180-184 23.39642571225798 21.0 15.0 32.0 13.8 35.8 185-189 22.451862054667878 22.2 13.2 31.0 12.2 36.0 190-194 22.495232529128188 22.4 13.8 30.8 13.0 35.4 195-199 21.277835199790758 16.6 13.6 28.2 12.4 35.4 200-204 20.004291909501042 19.8 12.2 25.8 10.0 32.4 205-209 19.367320129797804 15.6 12.6 25.6 12.0 31.4 210-214 18.449621124475563 15.2 12.2 24.6 12.0 30.0 215-219 18.472597089575633 14.0 13.4 24.6 12.6 27.2 220-224 17.065645326281448 13.4 12.2 24.2 12.0 25.8 225-229 17.80382269262367 14.0 14.0 24.0 13.0 26.0 230-234 17.349473350445255 13.4 12.6 24.0 12.4 26.0 235-239 17.07332343316731 13.4 12.4 24.0 12.0 26.0 240-244 16.87182119101153 13.2 12.0 24.0 12.0 26.0 245-249 17.333543188895945 13.6 12.2 24.0 12.0 26.0 250-251 16.097528866583573 13.5 12.5 19.0 12.0 25.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -5.652984993415632 1101 2 -1.365688658462556 1101 3 -0.32422291685839255 1101 4 0.09002585815005304 1101 5 0.320717068802562 1101 6 0.429275592087734 1101 7 0.397952183578937 1101 8 0.5399062093219626 1101 9 0.35848717021384857 1101 10-14 0.34628688190773005 1101 15-19 0.6868277447589648 1101 20-24 0.6141190376666756 1101 25-29 0.3888127918686237 1101 30-34 0.40679793885894355 1101 35-39 0.3989765310029654 1101 40-44 0.6743712624127554 1101 45-49 0.30888925101925935 1101 50-54 0.5099846872479787 1101 55-59 1.1293065335649999 1101 60-64 0.675140813895915 1101 65-69 0.3527723778566312 1101 70-74 0.6044705722824943 1101 75-79 0.5159725557867105 1101 80-84 0.6634933409489179 1101 85-89 0.21732147002747837 1101 90-94 0.4080698589000562 1101 95-99 0.03262142129424461 1101 100-104 0.12012395787149899 1101 105-109 0.7273237013729066 1101 110-114 0.8483944208176695 1101 115-119 0.6253307667201291 1101 120-124 0.2740861717363785 1101 125-129 0.6668985636524383 1101 130-134 0.8339603974610554 1101 135-139 0.6996065958830151 1101 140-144 0.8219714454323004 1101 145-149 0.5910988907267622 1101 150-154 1.0704702302968059 1101 155-159 0.32839208033556844 1101 160-164 0.5290373723763686 1101 165-169 0.38123454169021187 1101 170-174 0.792599403598345 1101 175-179 0.19670037114104488 1101 180-184 0.10383515154945755 1101 185-189 0.6633517423036004 1101 190-194 0.5465611041086404 1101 195-199 0.6208849419499529 1101 200-204 0.9300296680576459 1101 205-209 0.07825684547234957 1101 210-214 0.04225343109591151 1101 215-219 -0.22787772423404817 1101 220-224 -0.11850826387616209 1101 225-229 0.1283896744320785 1101 230-234 0.008217539226475168 1101 235-239 0.2706600866737823 1101 240-244 0.17298857966623515 1101 245-249 -0.010013275471155936 1101 250-251 -0.016730666892282642 1102 1 -1.1926207544602718 1102 2 -0.77966116704253 1102 3 -2.11759397141336 1102 4 -4.516131916361967 1102 5 -3.675211666030812 1102 6 -4.7247723184162425 1102 7 -2.647407598600793 1102 8 -3.4711193683068515 1102 9 -1.922105576137433 1102 10-14 -0.6013788453148479 1102 15-19 -2.0745658187125002 1102 20-24 -6.1220349535594 1102 25-29 -2.7558251167732983 1102 30-34 -0.6534282860280953 1102 35-39 -0.5015301568198041 1102 40-44 -1.9244149634305785 1102 45-49 -1.4800358545624164 1102 50-54 -1.2260440809485473 1102 55-59 -2.1046519820573337 1102 60-64 -3.3061634993728433 1102 65-69 -2.008371641204725 1102 70-74 -3.880108164727936 1102 75-79 -1.4475695419503651 1102 80-84 -0.07718548842559159 1102 85-89 -0.5108273676900268 1102 90-94 -2.425616121330915 1102 95-99 -1.0392346916639426 1102 100-104 -0.34210592110532545 1102 105-109 -0.5599296978919099 1102 110-114 -1.3976232494501133 1102 115-119 -0.7398670169631352 1102 120-124 0.3294401329156216 1102 125-129 0.19460528629734597 1102 130-134 0.48935748845872595 1102 135-139 0.12325881051869914 1102 140-144 0.06841096820296855 1102 145-149 -0.5221546689134513 1102 150-154 -0.40998802460581274 1102 155-159 0.20186438844820032 1102 160-164 0.08901658008023716 1102 165-169 -0.7278292382482725 1102 170-174 0.16858229391572266 1102 175-179 -0.6301995464606307 1102 180-184 -1.2855439485746238 1102 185-189 -0.05131055496711312 1102 190-194 0.3390465579981843 1102 195-199 -0.9295307195375564 1102 200-204 0.5365338227930714 1102 205-209 0.521568745259767 1102 210-214 -0.01930557157559676 1102 215-219 -0.5782186259248654 1102 220-224 -0.09558191690175732 1102 225-229 0.1472099502686106 1102 230-234 0.04384673170166309 1102 235-239 0.20506407669038396 1102 240-244 0.08649269192892106 1102 245-249 0.1859652462820165 1102 250-251 0.04841067984732561 1103 1 0.8367611898813685 1103 2 0.32794125617790826 1103 3 0.5713202181330885 1103 4 0.596056796358166 1103 5 0.6484583027731823 1103 6 0.5015743925784761 1103 7 0.48937239698436485 1103 8 0.49823167128259627 1103 9 0.2831100137090701 1103 10-14 0.4470185926809407 1103 15-19 0.49282902430567077 1103 20-24 0.5339923323170481 1103 25-29 0.4425979903201096 1103 30-34 0.2010776514837076 1103 35-39 0.3171353952243763 1103 40-44 0.4519997714582473 1103 45-49 0.11748311487735918 1103 50-54 0.475300374043627 1103 55-59 0.7832094702841914 1103 60-64 0.8228619204013299 1103 65-69 0.1795277174505827 1103 70-74 0.6210556820198363 1103 75-79 0.5079520586801465 1103 80-84 0.5759501092961656 1103 85-89 0.15051097903848643 1103 90-94 0.24047721426501312 1103 95-99 0.1368138210340888 1103 100-104 0.1411366666290519 1103 105-109 0.24037973275655844 1103 110-114 -0.18938333486650194 1103 115-119 0.4684788187099507 1103 120-124 0.6622556585715742 1103 125-129 0.8398762390071752 1103 130-134 0.747481364805946 1103 135-139 0.8456468362591671 1103 140-144 0.7120365331729168 1103 145-149 0.567916745497616 1103 150-154 1.1060668846203185 1103 155-159 1.2059445376242017 1103 160-164 0.5700111957572283 1103 165-169 -0.14955602092322096 1103 170-174 0.6904197192554626 1103 175-179 0.3952184286982394 1103 180-184 0.997473736732136 1103 185-189 0.6373839678829079 1103 190-194 0.3289282225348167 1103 195-199 0.4872744705280141 1103 200-204 1.280904640372622 1103 205-209 0.02915533224959077 1103 210-214 0.35087010966100607 1103 215-219 0.18602715354508348 1103 220-224 0.018392596920573823 1103 225-229 -0.29589035153399124 1103 230-234 0.3200109637981505 1103 235-239 0.83103025908812 1103 240-244 0.4446077837967053 1103 245-249 0.41101297343263 1103 250-251 0.08531539979972891 2112 1 0.7510122945097883 2112 2 0.25294892559136883 2112 3 0.5114891893747853 2112 4 0.5381841559715177 2112 5 0.5021758592089647 2112 6 0.2409782476444704 2112 7 0.3055236426924637 2112 8 0.5095833571244768 2112 9 0.36509358258555125 2112 10-14 0.3815083780014277 2112 15-19 0.41013086555842193 2112 20-24 0.5670304929156416 2112 25-29 0.30927017255967115 2112 30-34 0.07552619652710746 2112 35-39 0.2748921205614536 2112 40-44 0.39648539838848507 2112 45-49 0.06657518187287081 2112 50-54 0.29735641783055655 2112 55-59 0.421502957895477 2112 60-64 0.6184101483180058 2112 65-69 5.524765769919782E-4 2112 70-74 0.42642742812802936 2112 75-79 0.3509429938059938 2112 80-84 0.6457977603838359 2112 85-89 0.25398185920970917 2112 90-94 0.24296398647130957 2112 95-99 -0.03587148575724086 2112 100-104 -0.046742361303682145 2112 105-109 -0.11282199423233408 2112 110-114 -0.4455512911368338 2112 115-119 0.3570963534423406 2112 120-124 0.3695230683311408 2112 125-129 0.49226459405241485 2112 130-134 0.5771533062373173 2112 135-139 0.4657777904892697 2112 140-144 0.45087630997695527 2112 145-149 0.17285368442582794 2112 150-154 0.7671276300729346 2112 155-159 0.10396988974148869 2112 160-164 0.20587936251139638 2112 165-169 -0.191869603305463 2112 170-174 0.5636752848880953 2112 175-179 0.16417307813875937 2112 180-184 0.04938780619648142 2112 185-189 0.39004065299593904 2112 190-194 -0.021105426063581234 2112 195-199 0.8509133996675686 2112 200-204 1.236416745808814 2112 205-209 0.042186269932141585 2112 210-214 0.1251298705998778 2112 215-219 -0.10565326786242224 2112 220-224 -0.007594373416942801 2112 225-229 -0.07796303514710701 2112 230-234 -0.00129047901917545 2112 235-239 0.4659294250433419 2112 240-244 0.22756808133565087 2112 245-249 0.2109929543976854 2112 250-251 0.023411084918858904 1113 1 0.7397422279819033 1113 2 0.24959597041200965 1113 3 0.4399752895857212 1113 4 0.4645447279740864 1113 5 0.40615121294897705 1113 6 0.2784023686745485 1113 7 0.2968019050551476 1113 8 0.3804484721452468 1113 9 0.2742446010136419 1113 10-14 0.3528460098663544 1113 15-19 0.38510666769615653 1113 20-24 0.2796930602957559 1113 25-29 0.19760828713183543 1113 30-34 -0.013075866225371158 1113 35-39 0.11884989744098107 1113 40-44 0.4651882050073226 1113 45-49 -0.11095828234390837 1113 50-54 0.32675058247948385 1113 55-59 0.35730886383523597 1113 60-64 0.5005724772725628 1113 65-69 0.027171100177170615 1113 70-74 0.2922744890395137 1113 75-79 0.17711292900082043 1113 80-84 0.49759364849763443 1113 85-89 0.07565631544024853 1113 90-94 0.1503732445124868 1113 95-99 -0.043425720057207684 1113 100-104 -0.10378727219208272 1113 105-109 -0.3910520313949952 1113 110-114 -0.6566022621591081 1113 115-119 0.1974406109341622 1113 120-124 0.31795960080979313 1113 125-129 0.4618120436218156 1113 130-134 0.3924375337273496 1113 135-139 0.4033294555069915 1113 140-144 0.40959541064804483 1113 145-149 -0.20854032560183455 1113 150-154 0.5019165239120582 1113 155-159 0.16709528710223864 1113 160-164 0.18329393825422002 1113 165-169 -0.2353000431774035 1113 170-174 0.5648835016877776 1113 175-179 -0.34016163392464094 1113 180-184 0.21176925249948653 1113 185-189 0.31425235507194316 1113 190-194 -0.504207069909981 1113 195-199 0.7086279041674928 1113 200-204 1.2339936856217228 1113 205-209 0.29942370720931066 1113 210-214 0.36784415425022843 1113 215-219 -0.054094446082899594 1113 220-224 0.019747014587785827 1113 225-229 0.16114904273247177 1113 230-234 0.2804110186741191 1113 235-239 0.4052702170995488 1113 240-244 0.2984056940611488 1113 245-249 0.3748232820987738 1113 250-251 0.15409832376930055 2113 1 0.5977366250383831 2113 2 0.1844958450471914 2113 3 0.4754522000581076 2113 4 0.441460479730889 2113 5 0.2715382901697154 2113 6 0.12968498164288889 2113 7 0.23751117659401189 2113 8 0.30231695070303033 2113 9 0.23172411767004064 2113 10-14 0.3039308544860688 2113 15-19 0.5632496337318003 2113 20-24 0.45885766150563967 2113 25-29 0.22247772204130456 2113 30-34 0.003101912419793962 2113 35-39 -0.32610323928001606 2113 40-44 0.3366178060512297 2113 45-49 -0.02938531937720512 2113 50-54 0.22801786290289527 2113 55-59 -8.537917177475304 2113 60-64 -0.6540194883594026 2113 65-69 -0.32398035314044193 2113 70-74 0.27339538027102606 2113 75-79 0.14382592560817642 2113 80-84 0.49340581351596313 2113 85-89 0.1565862769657187 2113 90-94 0.005556185270179981 2113 95-99 -0.1991336284183376 2113 100-104 -0.23072865258761155 2113 105-109 0.3221755918863245 2113 110-114 -0.10207003809394166 2113 115-119 0.1046083766152286 2113 120-124 0.1947513675864414 2113 125-129 0.0940270015464435 2113 130-134 0.05118774616962085 2113 135-139 0.40473861763866026 2113 140-144 0.15042968129340295 2113 145-149 -0.6282772489039701 2113 150-154 0.5231676627492341 2113 155-159 -0.08213150155835436 2113 160-164 0.37965514962834135 2113 165-169 0.2696048264755717 2113 170-174 0.31360710716882423 2113 175-179 0.20470638367418914 2113 180-184 0.22429678165431355 2113 185-189 0.2927036674510042 2113 190-194 0.4409191736241702 2113 195-199 0.617193347135423 2113 200-204 0.8824615416026163 2113 205-209 0.13832253567845143 2113 210-214 0.058476193654261266 2113 215-219 -0.17765319920478717 2113 220-224 -0.02226029757125403 2113 225-229 0.12352472104954515 2113 230-234 -0.006403309412078784 2113 235-239 0.32937061672625845 2113 240-244 0.23437728794825574 2113 245-249 0.3815511505233786 2113 250-251 0.15745545044148201 1112 1 0.7029191819453224 1112 2 0.24967691241741008 1112 3 0.24565567191093507 1112 4 0.36171431867639825 1112 5 0.2824272838181052 1112 6 0.3842558815451653 1112 7 0.2538844596787655 1112 8 0.45644254122297667 1112 9 0.28806912827050724 1112 10-14 0.2707155748612706 1112 15-19 0.6989040049736346 1112 20-24 0.4634944376119918 1112 25-29 0.1626248435839699 1112 30-34 -0.021792833504179043 1112 35-39 -0.3027683896064062 1112 40-44 0.31777950275532874 1112 45-49 0.028200595764310776 1112 50-54 0.35797388884277836 1112 55-59 -2.878263133997482 1112 60-64 0.34164321440055545 1112 65-69 -0.32192317537185033 1112 70-74 0.28874081445302124 1112 75-79 0.3457242112561332 1112 80-84 0.5493482343442153 1112 85-89 0.1269537804117391 1112 90-94 0.08639098457821603 1112 95-99 0.051299941389352455 1112 100-104 -0.1384207073246877 1112 105-109 0.19513412515901507 1112 110-114 -0.18996582309878818 1112 115-119 0.15689088401218854 1112 120-124 0.3384484329154489 1112 125-129 0.25582085703767987 1112 130-134 0.6498391141877029 1112 135-139 0.4320050485194429 1112 140-144 0.042192461287463345 1112 145-149 -0.8243745050184188 1112 150-154 0.4910937849763428 1112 155-159 0.11408978955157068 1112 160-164 0.10198880424183798 1112 165-169 -0.24925042086782412 1112 170-174 0.24468169128813244 1112 175-179 -0.1543638608074147 1112 180-184 -0.11085828909962814 1112 185-189 0.20638977692309268 1112 190-194 0.1588817793571451 1112 195-199 0.35043782917101396 1112 200-204 1.0761288338561528 1112 205-209 0.09616104618178767 1112 210-214 0.24383947851778132 1112 215-219 -0.024819191960556708 1112 220-224 0.008852828490379494 1112 225-229 -0.3883734931103362 1112 230-234 0.16330807834870953 1112 235-239 0.6685320453770522 1112 240-244 0.5396271948600635 1112 245-249 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2110 5 0.08541348967085938 2110 6 0.2261850310379998 2110 7 0.20526235026435558 2110 8 -0.09853795120559283 2110 9 0.015350571930568435 2110 10-14 0.36913780562331766 2110 15-19 0.15422892637286623 2110 20-24 0.7530727242165725 2110 25-29 0.37309365431698893 2110 30-34 0.3105671035145079 2110 35-39 0.29596236999411474 2110 40-44 -0.1899167172265379 2110 45-49 0.25779162250245946 2110 50-54 0.19307096685486158 2110 55-59 0.6541196685439488 2110 60-64 0.48508430004731906 2110 65-69 0.23997711567038493 2110 70-74 -0.21359012034961466 2110 75-79 -0.05075066852103305 2110 80-84 -0.9100264313899693 2110 85-89 0.13285208425559603 2110 90-94 0.3303616779123999 2110 95-99 0.40071074916983207 2110 100-104 0.6339152963900219 2110 105-109 -1.936783209074779 2110 110-114 0.19481599285473905 2110 115-119 0.3727050800106859 2110 120-124 0.11375002751999119 2110 125-129 -0.07357200919294016 2110 130-134 -0.22574554314816098 2110 135-139 0.06359092006710299 2110 140-144 -0.24742717123033486 2110 145-149 0.033950959853982 2110 150-154 -0.25016803261824805 2110 155-159 0.04976291212073036 2110 160-164 -0.44530964826920183 2110 165-169 0.7677005217005934 2110 170-174 -0.5020708170362056 2110 175-179 -0.2748672744723173 2110 180-184 -0.3816566080286101 2110 185-189 -0.3736914906212796 2110 190-194 0.13324135007213656 2110 195-199 -0.6746976029909781 2110 200-204 0.5602328253489368 2110 205-209 -0.40564476065933164 2110 210-214 -0.2423337501208458 2110 215-219 -0.08485312903385278 2110 220-224 -0.14001445842551163 2110 225-229 0.04916356936814026 2110 230-234 -0.3290836954270162 2110 235-239 -0.30755813016245526 2110 240-244 -0.37370813572463746 2110 245-249 -0.25491249359032864 2110 250-251 -0.09181403166160607 2109 1 0.6869124849735506 2109 2 0.19995351091059987 2109 3 -0.11097274129539869 2109 4 0.17087859732856003 2109 5 -0.07663631122442638 2109 6 0.17202904927361828 2109 7 0.053626447179773606 2109 8 0.0022626922033595065 2109 9 0.011873937195403528 2109 10-14 0.3214620923702185 2109 15-19 0.09078722089581248 2109 20-24 0.27949828871262383 2109 25-29 -0.06838842556145863 2109 30-34 0.30161035173138373 2109 35-39 0.05107165734087005 2109 40-44 0.003758933057838476 2109 45-49 0.31120480804906947 2109 50-54 0.08811335149433575 2109 55-59 0.5315947238601098 2109 60-64 -0.18020060327348375 2109 65-69 0.2809006614791443 2109 70-74 -0.8029827188042233 2109 75-79 -0.29091128979133174 2109 80-84 0.42988689553528303 2109 85-89 0.3508644496979727 2109 90-94 0.9782891552211552 2109 95-99 0.29995793517042557 2109 100-104 0.38615724919375083 2109 105-109 0.6751111348022718 2109 110-114 0.9236543834800273 2109 115-119 0.4060482624039672 2109 120-124 -0.16364355297034194 2109 125-129 -0.06523483152219 2109 130-134 0.13870260957169833 2109 135-139 0.5095831837009932 2109 140-144 0.26262198450087126 2109 145-149 0.5425718059986373 2109 150-154 0.12906745568772848 2109 155-159 -0.46160317573856346 2109 160-164 -0.4489756701104284 2109 165-169 -0.6752237081032959 2109 170-174 0.22625605656072523 2109 175-179 0.5514174674407855 2109 180-184 0.2913864878108896 2109 185-189 0.3790281031975091 2109 190-194 0.07204952985200563 2109 195-199 -0.22767357847295955 2109 200-204 0.697346478926594 2109 205-209 -0.07785908390555818 2109 210-214 -0.15239871743405686 2109 215-219 0.5584802329929062 2109 220-224 0.04810930506236488 2109 225-229 0.10362113699277842 2109 230-234 -0.22756782108697848 2109 235-239 -0.312644847296049 2109 240-244 -0.23917234396941467 2109 245-249 -0.1849237103003638 2109 250-251 0.07963247713043842 2108 1 0.9642276970247856 2108 2 0.4193184622808417 2108 3 0.19159811237955182 2108 4 0.2913469121915959 2108 5 0.27630236491312843 2108 6 0.43917270947871856 2108 7 0.3362277831445013 2108 8 0.09021032423431308 2108 9 0.18722274246241 2108 10-14 0.5245138350020468 2108 15-19 0.25090541116360754 2108 20-24 0.6183142789458316 2108 25-29 0.2778305296098793 2108 30-34 0.3496360248976771 2108 35-39 0.40444098530455364 2108 40-44 0.17970460349181394 2108 45-49 0.5929627148088983 2108 50-54 0.13753761285023103 2108 55-59 1.2588872259933552 2108 60-64 0.38691601562997846 2108 65-69 0.42662641376654165 2108 70-74 -0.6600136395143466 2108 75-79 -0.17009315008242254 2108 80-84 0.695650902456542 2108 85-89 0.3499309169387672 2108 90-94 0.804720173531301 2108 95-99 0.3247476405407852 2108 100-104 0.6484118792307925 2108 105-109 0.4855225729369117 2108 110-114 0.22069687924872383 2108 115-119 0.15979916017621676 2108 120-124 0.11587915179720909 2108 125-129 0.04063378736839951 2108 130-134 0.15230306331865862 2108 135-139 -2.7809951187083897 2108 140-144 0.04484095075311245 2108 145-149 1.0731320084286295 2108 150-154 -0.5452834937960773 2108 155-159 -1.5163826873509585 2108 160-164 -0.5898583811575016 2108 165-169 0.6823718625281003 2108 170-174 0.323276059201703 2108 175-179 1.16203661356451E-4 2108 180-184 -0.19001933161947804 2108 185-189 -0.2526569078749148 2108 190-194 -0.3342716508326191 2108 195-199 -0.517701215959363 2108 200-204 0.44321340592077973 2108 205-209 0.256602933734289 2108 210-214 0.06637208693234697 2108 215-219 0.5187977526645895 2108 220-224 0.3593300081577162 2108 225-229 0.17086112721599278 2108 230-234 -0.08257397507350106 2108 235-239 -0.3206663401441645 2108 240-244 -0.39002831266110505 2108 245-249 -0.25690332340107247 2108 250-251 0.21914173178131335 >>END_MODULE >>Per sequence quality scores pass #Quality Count 12 62.0 13 1190.0 14 1538.0 15 5021.0 16 28638.0 17 62580.0 18 99686.0 19 161096.0 20 249493.0 21 370246.0 22 558931.0 23 862899.0 24 1326313.0 25 1875513.0 26 2332809.0 27 2595870.0 28 2653893.0 29 2476839.0 30 2036920.0 31 1419186.0 32 774040.0 33 313216.0 34 94365.0 35 15541.0 36 945.0 37 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 1.37836456876567 2.059878334372127 1.4729807025514954 95.0887763943107 2 0.9229342902935995 1.341006380374971 1.0810790324534372 96.654980296878 3 2.448083561850763 1.1301762563265896 66.77174703082385 29.649993150998792 4 95.61221432614171 1.4624229536584716 1.894306252781253 1.0310564674185654 5 1.4755155466913121 1.699886168271026 95.75595721596542 1.0686410690722388 6 0.9641562702372234 1.2016293289046898 96.68206129194066 1.152153108917429 7 1.0662440417011885 1.1034102710211056 79.4443434608478 18.3860022264299 8 96.33832166049912 1.164679668792835 1.55514902890121 0.941849641806835 9 0.91386791571973 4.4998848491676675 1.1064766941261657 93.47977054098644 10-14 1.0080184356312107 2.0123394442011104 20.317897218497286 76.66174490167039 15-19 22.847898867692507 16.899385539014425 26.44945759503537 33.8032579982577 20-24 21.075875465027003 1.6297364485632626 42.62384817789743 34.6705399085123 25-29 17.888917814003573 7.252556267264319 25.61406746947888 49.24445844925323 30-34 38.934354476837456 20.2877121929104 22.740335832886537 18.037597497365606 35-39 24.07664856787951 34.60644428257537 18.74445675115376 22.572450398391364 40-44 32.331112071562735 13.56399627481274 26.218529848478827 27.886361805145693 45-49 27.005216512358643 24.824944402008363 6.374806189016387 41.795032896616604 50-54 29.142944586190634 20.496808778888795 14.806643811724376 35.553602823196194 55-59 20.11789672032028 1.312736862909825 40.949726709055184 37.61963970771471 60-64 20.42986329905486 19.0711986529789 3.093326907134287 57.40561114083196 65-69 38.4755703288569 4.45916393161906 31.757253874878423 25.308011864645625 70-74 18.691645365362344 2.3011128064214343 43.95003531800801 35.05720651020821 75-79 19.63472059200571 9.892818422321866 27.744454831563054 42.72800615410937 80-84 1.9241081635625965 34.11522529389771 38.9801543260379 24.9805122165018 85-89 23.17694920039449 21.115027240222652 20.16739618496605 35.54062737441681 90-94 1.3692135353195585 19.14754225203724 56.772282055208315 22.71096215743489 95-99 15.700287116627587 20.681097362083683 6.826889488818409 56.79172603247032 100-104 1.0330321692393858 36.61748330731304 21.23520543139823 41.114279092049344 105-109 41.26481241095129 24.365148285182862 10.638810747601337 23.731228556264508 110-114 3.3105546824699186 16.919443785302935 40.1605063309332 39.609495201293946 115-119 14.079529430549478 22.545029783434238 26.460848150973938 36.91459263504234 120-124 1.2566920500544598 1.7456167253643053 35.28365225856336 61.71403896601788 125-129 22.387022858043164 15.066623333136944 15.737120616891485 46.809233191928406 130-134 4.670251969906134 21.63209803733663 30.07384665452993 43.623803338227304 135-139 23.113175475053172 28.34578089466807 13.978174056770959 34.5628695735078 140-144 34.88521413600379 23.197996774201645 12.075493466476145 29.84129562331842 145-149 9.993604809726477 16.816301715557906 44.59619022277638 28.59390325193924 150-154 34.954174693878194 23.334302480539414 11.071935382048508 30.639587443533884 155-159 13.877564862354763 17.104188148240247 36.18889678218025 32.82935020722474 160-164 43.452019657987016 13.958037058043157 6.853928154444952 35.73601512952487 165-169 32.5205323605832 22.07877695099201 37.785888950890026 7.614801737534756 170-174 30.199889933622032 6.94253744592353 10.244603599842907 52.61296902061153 175-179 32.935774284877404 13.60468569138563 39.72394218369981 13.735597840037158 180-184 6.502262089988345 26.026501869312195 21.957006976137176 45.51422906456229 185-189 23.94001505451318 22.91956358745121 18.103668825123368 35.036752532912246 190-194 11.626962886091832 17.569732208728812 36.498409619098574 34.304895286080786 195-199 27.386929585622877 25.708829295277397 10.864581193789524 36.039659925310204 200-204 18.047247465346274 5.484332445760102 20.07930395702485 56.389116131868775 205-209 32.67949022167876 22.433159974604308 7.480581002027334 37.406768801689594 210-214 13.505720587090778 3.009761739020832 35.930730889386545 47.553786784501845 215-219 21.147545106813165 18.677340969169848 22.464558572151336 37.71055535186566 220-224 19.771108003999245 18.83068771896562 21.099485446327726 40.29871883070741 225-229 9.813330632124666 26.38398281700527 33.39141128850262 30.411275262367443 230-234 24.721350490142875 19.749352642643924 11.525612434340768 44.00368443287243 235-239 20.099491894183693 27.76450717141861 6.370878411106536 45.765122523291154 240-244 23.674537628432308 12.096105933056194 28.040544315203487 36.18881212330801 245-249 7.421586784521271 11.072897473220035 20.498436606528937 61.00707913572976 250-251 33.77144382830777 2.896677215474136 12.25328429057869 51.07859466563941 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 15.5 1 1.9999999999999998 2 0.6666666666666666 3 0.6666666666666666 4 0.3333333333333333 5 0.6666666666666666 6 0.3333333333333333 7 0.6666666666666666 8 0.3333333333333333 9 0.6666666666666666 10 0.3333333333333333 11 0.3333333333333333 12 0.3333333333333333 13 0.0 14 0.0 15 0.3333333333333333 16 0.6666666666666666 17 0.3333333333333333 18 0.6666666666666666 19 3.0 20 3.3333333333333335 21 1.3333333333333333 22 2.333333333333333 23 5.999999999999998 24 4.666666666666666 25 7.33333333333333 26 13.33333333333334 27 19.666666666666664 28 44.000000000000036 29 107.66666666666622 30 221.0000000000019 31 309.3333333333332 32 344.3333333333312 33 380.9999999999958 34 341.3333333333314 35 469.6666666666574 36 978.0000000000413 37 1891.6666666665158 38 2972.333333333567 39 7649.6666666641795 40 17728.00000000703 41 25806.999999977637 42 31917.666666622074 43 41564.666666682984 44 57390.66666679813 45 69705.3333334634 46 85653.99999989798 47 91833.99999980805 48 83788.33333325846 49 89639.66666650664 50 126681.33333263428 51 162553.3333334866 52 227479.3333353762 53 340537.66666515527 54 505347.66665556206 55 712498.0000097034 56 901621.666698387 57 1052439.333381262 58 1116570.6666996635 59 1069724.3333772374 60 945003.0000367706 61 760187.0000152552 62 557734.6666583533 63 380938.3333294703 64 246838.66666927296 65 155309.33333327578 66 97244.66666639598 67 62687.33333350334 68 43137.66666669443 69 29257.66666663175 70 18761.00000000327 71 11578.000000003178 72 7047.666666664727 73 4330.333333333865 74 2719.0000000001187 75 1712.9999999998897 76 1092.6666666666974 77 773.3333333333513 78 545.6666666666588 79 432.3333333333262 80 357.9999999999971 81 306.6666666666667 82 262.3333333333359 83 227.66666666666876 84 194.66666666666782 85 183.33333333333417 86 159.33333333333348 87 146.9999999999998 88 116.99999999999942 89 115.6666666666661 90 113.66666666666613 91 105.99999999999957 92 97.99999999999969 93 103.33333333333294 94 95.99999999999972 95 110.33333333333285 96 114.33333333333279 97 124.33333333333265 98 149.66666666666654 99 325.6666666666656 100 869.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 9.844054911910228E-7 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.714391924606746 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 9.844054911910228E-7 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-154 0.0 155-159 0.0 160-164 0.0 165-169 0.0 170-174 0.0 175-179 0.0 180-184 0.0 185-189 0.0 190-194 0.0 195-199 0.0 200-204 2.1616796438381556 205-209 0.0 210-214 9.844054911910228E-7 215-219 0.0 220-224 0.0 225-229 0.0 230-234 0.0 235-239 0.0 240-244 0.0 245-249 2.9532164735730685E-6 250-251 2.461013727977557E-6 >>END_MODULE >>Sequence Length Distribution pass #Length Count 251 2.0316831E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 26.014396872928224 #Duplication Level Percentage of deduplicated Percentage of total 1 88.10425974159499 22.91979179113405 2 5.452370285474059 2.836802490089663 3 1.8691412720992582 1.458737485918801 4 1.1162360565630234 1.161528311172114 5 0.658774495428161 0.8568810586915613 6 0.4141829681392443 0.6464832066709013 7 0.29526763975412945 0.5376846695007707 8 0.247958622360943 0.5160395208129686 9 0.21115721427445946 0.49438148172459606 >10 1.2525283459807535 6.7294865889770445 >50 0.19941760966875016 3.637842557553509 >100 0.14161134933310696 7.368653552242457 >500 0.015526911435086888 2.815065249403937 >1k 0.015230051449319196 8.280306550443212 >5k 0.0025013231408663665 4.512436891676542 >10k+ 0.0038361133038727524 35.22787859398787 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CCCGTTTGCTCCCCTGGCTTTCGCGCCTCAGCGTCAATACCGGTCCAGGA 310382 1.5277087258342603 No Hit CCTGTTCGCTCCCCACGCTTTCGCGTCTCAGCGTCAGTACCGTCCCAGAG 291984 1.4371532646995981 No Hit CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTGTTGGCCCAGAA 212869 1.0477470625217093 No Hit CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAGTACCGGACCAGTG 199873 0.9837803937041166 No Hit CCTGTTTGCTCCCCACGCTTTCGCGTCTCAGCGTCAGTAACGTCCCAGCG 179039 0.8812348736867478 No Hit CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAGTACCGGGCCAGTG 178073 0.8764801951642951 No Hit CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTGCAGGCCCAGGG 125010 0.6153026522689489 No Hit CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTGTTAGCCCAGGG 110247 0.5426387609366835 No Hit CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTATCGGCCCAGAG 106381 0.523610202791961 No Hit CCCGTTTGCTCCCCTGGCTTTCGCGCCTCAGCGTCAATGCCGGTCCAGGA 103196 0.507933545344744 No Hit CCTGTTCGCTCCCCACGCTTTCGCGTCTCAGCGTCAGTACCGTTCCAGAG 100951 0.4968835937061248 No Hit CCTGTTTGCTCCCCACGCTTTCGCACATGAGTGTCAGTATTGGGCCAGGA 100216 0.4932659035259977 No Hit CCCGTTTGCTCCCCTAGCTTTCGCGCCTCAGCGTCAGTGTCGGTCCAGGA 97534 0.4800650258891261 No Hit CCCGTTTGCTCCCCTAGCTTTCGCGCCTCAGCGTCAAGAACAGGCCAGGT 91167 0.44872647707705987 No Hit CCCGTTCGCTCCCCTAGCTTTCGCGCCTCAGCGTCAGTGTCAGCCCAGCA 90425 0.44507433270474117 No Hit CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTGTCAGCCCAGAG 84788 0.4173288639355222 No Hit CCTGTTTGCTCCCCACGCTTTCGCACATGAGCGTCAGTATTGGTCCAGGA 77458 0.38125040268337124 No Hit CCTGTTTGCTCCCCACGCTTTCGCGTCTCAGCGTCAGTATCCAACCAGGG 76348 0.37578695220726105 No Hit CCTGTTTGCTCCCCACGCTTTCGTGCCTGAACGTCGGTACTGGTCCAGGA 68105 0.33521467988782305 No Hit CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAATACCAGTCCAGTG 66573 0.3276741338252998 No Hit CCTGTTTGCTCCCCACGCTGTCGCACCTGAGTGTCAGTATTGGGCCAGGT 65981 0.32476029357137437 No Hit CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAGTATCGGGCCAGTG 65900 0.32436160934744207 No Hit CCTGTTTGCTCCCCATGCTTTCGTACCTCAGCGTCAGTATTAGGCCAGAT 64272 0.3163485486491471 No Hit CCCGTTTGCTCCCCTGGCTTTCGCTCCTCAGCGTCAGTGTTGGTCCAGGA 62005 0.30519031240649686 No Hit CCCGTTTGCTCCCCTGGCCTTCGTGCCTCAGCGTCAGGAACTGTCCAGAG 58933 0.29006984406180275 No Hit CCTGTTTGCTCCCCACGCTGTCGCGCCTCAGCGTCAGCAACAGCCCAGCA 58130 0.28611745601467076 No Hit CCTGTTTGCTCCCCACGCTGTCGCGCCTCAGTGTCAGCAACGGCCCAGCA 56744 0.279295525960717 No Hit CCCGTTTGCTCCCCTAGCTTTCGCGCCTCAGCGTCAATGCCGGTCCAGGA 55571 0.2735219877548817 No Hit CCCGTTTGCTCCCCTAGCTTTCGCGCCTCAGCGTCAAGAACAGGCCAGGA 53818 0.2648936736245923 No Hit CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTTACTGCCCAGAG 53726 0.26444084709864446 No Hit CCGGTTTGCTCCCCCAGCTTTCATCCCTCACCGTCGAGCGCGTTCTGGCA 53700 0.2643128743847896 No Hit CCTGTTCGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTACCGTCCCAGCG 53214 0.26192076904119543 No Hit CCTGTTTGCTCCCCACGCTTTCGCGTCTCAGCGTCAGTACCGTCCCAGCG 52081 0.2563441119335983 No Hit CCCGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGGAGATGCCCAGAG 51848 0.25519727953636073 No Hit CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAATTACGGGCCAGAT 51803 0.2549757883008428 No Hit CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTACCGTCCCAGCG 51592 0.2539372405076362 No Hit CCTGTTTGCTCCCCACGCTTTCGCGTCTCAGCGTCAGTACCGTCCCAGAG 51524 0.2536025426406313 No Hit CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAATATGTGTCCAGTG 50779 0.24993563218594475 No Hit CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTACAGGTCCAGGG 50370 0.2479225229564591 No Hit CCTGTTTGCTCCCCACGCTTTCGCACTTGAGTGTCAGTACTGGACCAGAG 50343 0.2477896282151483 No Hit CCTGTTTGCTCCCCACGCTGTCGCGCCTCAGCGTCAGCAACGGCCCAGCA 50224 0.24720390694788966 No Hit CCTGTTTGCTCCCCACGCTGTCGCGCCTCAGCGTCAGCCACAGCCCAGCG 50001 0.24610629482521165 No Hit CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAGTACCGATCCAGTG 49681 0.24453124603930604 No Hit CCCGTTTGCTCCCCTGGCTTTCGCTCCTCAGTGTCAGTGTCGGTCCAGGA 49355 0.24292666508866465 No Hit CCCGTTCGCTCCCCTAGCTTTCGCGCCTCAGCGTCAGTGTCGGCCCAGCA 48624 0.23932866301836145 No Hit CCTGTTTGATCCCCACACTTTCGTGCCTCAGCGTCAGTTACAGCCTAGTA 47902 0.2357749591951619 No Hit CCTGTTTGCTCCCCACGCTTTCGCACCTGAGCGTCAGTATTGGTCCAGGA 47536 0.2339734971462823 No Hit CCCGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGGAAATGCCCAGAG 47283 0.23272822419992564 No Hit CCCGTTTGCTCCCCTGGCTTTCGCTCCTCAGTGTCAGTGTTGGTCCAGGA 46550 0.22912037807471058 No Hit CCCGTTTGCTCCCCTGGCTTTCGCGCCTCAGCGTCAGTGTCAGCCCAGCA 45635 0.22461672295251162 No Hit CCTGTTTGCTCCCCACGCTTTCGCGTATCAGCGTCAGTATCCAACCAGGG 44799 0.22050190799933317 No Hit CCCGTTTGCTCCCCTGGCTTTCGCTCCTCAGTGTCAATGCCGGTCCAGGA 44699 0.22000970525373767 No Hit CCCGTTTGCTCCCCTGGCTTTCGCTCCTCAGCGTCAGTATTGGTCCAGGA 44377 0.2184248124129201 No Hit CCTGTTTGCTCCCCACGCTTTCGCACCTCAGTGTCAGTACCGGGCCAGTG 43566 0.21443304814614048 No Hit CCCGTTTGCTCCCCTGGCTTTCGCTCCTCAGCGTCAATGCCGGTCCAGGA 43486 0.2140392859496641 No Hit CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTGTTGGCCCAGGA 43345 0.21334528007837442 No Hit CCCGTTTGCTCCCCTGGCCTTCGTGCCTCAGTGTCAGGAACTGTCCAGAG 42939 0.21134693693125664 No Hit CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTTGTTGCCCAGAG 42646 0.20990478288666178 No Hit CCTGTTTGCTCCCCACGCTTTCGCGTCTCAGCGTCAGTCACCGTCCAGAG 42515 0.20925999728993167 No Hit CCTGTTCGCTCCCCACGCTTTCGCGTCTCAGCGTCAGTACCGTACTAGAG 42479 0.2090828043015173 No Hit CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTATCGGGCCAGTG 42084 0.20713860345641502 No Hit CCTGTTTGCTCCCCACGCTTTCGTGCATGAGTGTCAGTGTTGGCCCAGGA 41186 0.20271862280096734 No Hit CCTGTTTGCTCCCCTAGCTTTCGTGCCTCAGTGTCAGGAGTTTTCCAGGG 40757 0.20060707302236258 No Hit CCCGTTTGCTCCCCTGGCTTTCGTTCCTCAGTGTCAGTTGCAGTCCAGAA 40278 0.1982494218709601 No Hit CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTACAGGCCCAGGG 39369 0.19377529891349687 No Hit CCTGTTTGCTCCCCCGGCTTTCGTGCCTCAGTGTCAGGTGTTGTCCAGGG 39257 0.19322403183842993 No Hit CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGAAATGTTCCAGAG 38572 0.18985244303110066 No Hit CCTGTTCGCTCCCCACGCTTTCGCGCCTTAGCGTCAGTACCGTCCCAGCG 38118 0.18761784256609704 No Hit CCTGTTTGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTACCGGCCCAGAG 37931 0.18669742343183346 No Hit CCCGTTCGCTCCCCTGGCTTTCGCGCATCAGCGTCAGTATCGGCCCAGTA 37319 0.1836851426287889 No Hit CCCGTTTGCTCCCCTAGCTTTCGCTCCTCAGCGTCAATGTCGGTCCAGGA 36897 0.18160804704237585 No Hit CCTGTTTGCTCCCCACGCTTTCGCGTCTCAGCGTCAGTCACTGTCCAGAA 36838 0.1813176474224745 No Hit CCGGTTTGCTCCCCCAGCTTTCATCCCTCACCGTCGAGCGCATTCTGGCA 35548 0.1749682320042924 No Hit CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAATACCGGTCCAGTG 35299 0.17374264716775958 No Hit CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAATATGTGTCCAGTA 35175 0.17313231576322113 No Hit CCCGTTTGCTACCCTAGCTTTCGCGCCTCAGCGTCAAGAACAGGCCAGGA 34418 0.16940634097906312 No Hit CCCGTTTGCTCCCCTAGCTTTCGCGCCTCAGCGTCAGTAATGGTCCAGGA 33383 0.16431204256214957 No Hit CCCGTTTGCTCCCCTAGCTTTCGCGCCTCAGCGTCAGTATTGGGCCAGAT 33005 0.16245151618379855 No Hit CCCGTTTGCTCCCCTGGCTTTCGCGCCTCAGCGTCAGTGTTGGCCCAGCA 32563 0.1602759800482664 No Hit CCTGTTTGCTCCCCACGCTTTCGCGTCTCAGCGTCAGTTACGTCCCAGCG 32513 0.16002987867546864 No Hit CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAATACGTGTCCAGTG 32504 0.15998558042836503 No Hit CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGAACCGGACCAGTA 32351 0.1592325102276039 No Hit CCTGTTTGCTCCCCACGCTTTCGCGTCTCAGCGTCAGTCATCGTCCAGAA 31726 0.15615624306763196 No Hit CCTGTTTGCTCCCCTAGCTTTCGCGCCTCAGCGTCAGGAACCGTCCAGAA 31306 0.15408899153613081 No Hit CCTGTTCGCTCCCCTAGCTTTCGCGCCTGAGCGTCAGGTAAGGCCCAGAG 31288 0.15400039504192362 No Hit CCCGTTCGCTCCCCTAGCTTTCGCGCCTCAGCGTCAAGAATGGGCCAGGT 30890 0.15204142811445348 No Hit CCCGTTTGCTCCCCTAGCTTTCGCGCCTCAGCGTCAAGAGTGGGCCAGGT 30684 0.15102749045852673 No Hit CCCGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGGAATTGCCCAGAG 30509 0.15016613565373457 No Hit CCTGTTCGCTCCCCATGCTTTCGCTCCTCAGCGTCAGTTAATGCCCAGAG 30331 0.14929001476657455 No Hit CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAGATCTGGACCAGTT 29830 0.14682407901114106 No Hit CCTGTTTGCTCCCCACGCTTTCGCACCTCAGTGTCAGTATCAGTCCAGGT 29593 0.1456575585040797 No Hit CCTGTTTGCTCCCCACGCTTTCGTGCCTGAGCGTCAGTGTCAGCCCAGGG 29392 0.14466823098543272 No Hit CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAGTATCGAGCCAGTG 28905 0.14227120361438259 No Hit CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAACAGTCGTCCAGTA 28900 0.1422465934771028 No Hit CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTGTTGGTCCAGGA 28830 0.14190205155518595 No Hit CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTGCTGGTCCAGGG 28775 0.1416313400451084 No Hit CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTATCGGGCCAGTG 28233 0.13896360116398074 No Hit CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTGCTGGTCCAGGT 28122 0.13841725611636974 No Hit CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAGTATTGGCCCAGGT 27674 0.13621218781610184 No Hit CCTGTTTGCTCCCCACGCTTTCGCGTCTCAGCGTCAGCAAAGATCCAGCA 27466 0.13518840610526317 No Hit CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTGTGGTCCAGTT 27079 0.13328358147980854 No Hit CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTAATGGCCCAGAG 26567 0.1307635034223595 No Hit CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAATACATGTCCAGTG 26455 0.13021223634729256 No Hit CCCGTTTGCTCCCCTGGCTTTCGTGCCTCAGCGTCAGGAAATGTCCAGAG 26432 0.1300990297158056 No Hit CCTGTTTGCTCCCCTAGCTTTCGTGCCTCAGCGTCGGTATCTGTCCAGAA 26330 0.12959698291529817 No Hit CCTGTTTGCTCCCCACGCTTTCGTGCCTGAACGTCGGTGCTGGTCCAGGA 25981 0.12787919533316983 No Hit CCTGTTCGCTCCCCTAGCTTTCGCGCCTCAGCGTCAGGTAAGGCCCAGAG 25965 0.12780044289387454 No Hit CCCGTTCGCTCCCCTGGCTTTCGCGCCTCAGCGTCAGTGTCAGCCCAGCA 25753 0.12675697307321204 No Hit CCTGTTCGCTCCCCACGCTTTCGCGTCTCAGCGTCAGTACCGTCCCAGAA 25072 0.12340507237570662 No Hit CCTGTTCGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTACCGTCCCAGAG 25067 0.12338046223842684 No Hit CCCGTTTGCTCCCCTAGCTTTCGCTCATCAGCGTCAATGCCGGTCCAGGA 24271 0.11946252838348657 No Hit CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTATTGGCCCAGGA 24117 0.11870453615526949 No Hit CCCGTTCGCTCCCCTAGCTTTCGCGCTTCAGCGTCAGTGTCAGCCCAGCA 24100 0.11862086168851825 No Hit CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTCCGGTCCAGTG 24050 0.11837476031572049 No Hit CCTGTTTGCTCCCCACGCTTTCGTGCCTGAGCGTCAGTGTTAGCCCAGGG 23753 0.11691291816130184 No Hit CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAACAATCGTCCAGTA 23656 0.11643548149807417 No Hit CCCGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTCACCGTCCAGAA 23066 0.11353148529906067 No Hit CCTGTTCGCTCCCCTAGCTTTCGCGCCTCAGCGTCAGGTCAGGCCCAGAG 22663 0.11154790823431075 No Hit CCTGTTTGCTCCCCACGCTGTCGCGCCTCAGCGTCAGTACTGGCCCAGTC 22410 0.11030263528795411 No Hit CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTGTCAGCCCAGGG 22293 0.10972675807560735 No Hit CCCGTTCGCTCCCCTGGCTTTCGCGCCTCAGCGTCAGTGTCGGCCCAGCA 22240 0.10946589062044174 No Hit CCTGTTTGCTCCCCACGCTTTCGTGCCTGAGCGTCAGTGTTGACCCAGGG 22058 0.10857008162345791 No Hit CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTGTTATCCCAGGG 21804 0.10731988664964531 No Hit CCCGTTTGCTCCCCTAGCTTTCGCTCCTCAGCGTCAATGCCGGTCCAGGA 21743 0.10701964297483205 No Hit CCCGTTTGCTCCCCTGGCCTTCGTGCCTCAGTGTCAGGAACTGCCCAGAG 21489 0.10576944800101945 No Hit CCTGTTTGCTCCCCTAGCTTTCGTGCCTCAGTGTCAGGAGTTGTCCAGGG 21369 0.10517880470630485 No Hit CCTGTTTGCTCCCCTAGCTTTCGTGCCTCAGTGTCAGGTGTTGTCCAGGG 21346 0.10506559807481788 No Hit CCTGTTTGCTCCCCACGCTGTCGCGCCTCAGCGTCAGCAACGGCCCAGCG 21268 0.10468167993325336 No Hit CCTGTTTGCTCCCCACGCTTTCGCGTCTCAGCGTCAGTCACCGTCCAGAA 21186 0.10427807368186505 No Hit CCCGTTTGCTCCCCTGGCTTTCGCTCCTCAGCGTCAATATTGGTCCAGGA 20651 0.10164478899292906 No Hit CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTGTTGACCCAGGG 20579 0.1012904030161003 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 3.150097571811273E-4 0.0 0.0 2 3.2485381209303757E-4 0.0 0.0 3 3.3469786700494777E-4 0.0 0.0 4 3.3469786700494777E-4 0.0 0.0 5 3.3469786700494777E-4 0.0 0.0 6 3.3469786700494777E-4 0.0 0.0 7 3.44541921916858E-4 0.0 0.0 8 3.6423003174067845E-4 0.0 0.0 9 3.6423003174067845E-4 0.0 0.0 10-14 3.8194933058211683E-4 0.0 0.0 15-19 4.1541911728261167E-4 0.0 0.0 20-24 4.410136600535782E-4 0.0 0.0 25-29 4.685770138069268E-4 0.0 0.0 30-34 5.985185386441419E-4 0.0 0.0 35-39 7.018811152191993E-4 0.0 0.0 40-44 8.229629906356952E-4 0.0 4.922027455955114E-6 45-49 0.0010060624119972252 0.0 4.922027455955114E-6 50-54 0.0011940838608147106 0.0 4.922027455955114E-6 55-59 0.0014854678862072535 0.0 4.922027455955114E-6 60-64 0.0018369006465624488 0.0 4.922027455955114E-6 65-69 0.002189317812408835 0.0 4.922027455955114E-6 70-74 0.002670692097601245 0.0 6.890838438337159E-6 75-79 0.0032140839287386896 0.0 9.844054911910228E-6 80-84 0.003949434830658383 0.0 9.844054911910228E-6 85-89 0.004965341297567519 0.0 9.844054911910228E-6 90-94 0.0062942887106754 0.0 9.844054911910228E-6 95-99 0.00793135504252607 0.0 9.844054911910228E-6 100-104 0.010126579287882052 0.0 9.844054911910228E-6 105-109 0.012699815241855383 0.0 9.844054911910228E-6 110-114 0.01579183288968639 0.0 1.0828460403101252E-5 115-119 0.01969992268971475 0.0 1.4766082367865342E-5 120-124 0.02419668697347534 0.0 1.4766082367865342E-5 125-129 0.028861784596229602 0.0 1.4766082367865342E-5 130-134 0.03384287638165618 0.0 1.4766082367865342E-5 135-139 0.039313217696204696 0.0 1.4766082367865342E-5 140-144 0.045209806588438914 0.0 1.4766082367865342E-5 145-149 0.05138990426213617 0.0 1.4766082367865342E-5 150-154 0.05743021635608427 4.922027455955114E-6 1.4766082367865342E-5 155-159 0.0640828286655532 4.922027455955114E-6 1.4766082367865342E-5 160-164 0.07045685422101508 4.922027455955114E-6 1.4766082367865342E-5 165-169 0.07687714683456293 4.922027455955114E-6 1.4766082367865342E-5 170-174 0.0831989988989917 7.875243929528183E-6 1.4766082367865342E-5 175-179 0.08946277103944016 9.844054911910228E-6 1.4766082367865342E-5 180-184 0.0958279369454813 9.844054911910228E-6 1.5750487859056365E-5 185-189 0.10247759603847668 9.844054911910228E-6 2.3625731788584548E-5 190-194 0.1082649159211887 9.844054911910228E-6 2.461013727977557E-5 195-199 0.11317906813321428 9.844054911910228E-6 2.461013727977557E-5 200-204 0.11881872719224765 9.844054911910228E-6 2.461013727977557E-5 205-209 0.12465526734951922 9.844054911910228E-6 2.9532164735730683E-5 210-214 0.12992675875484713 9.844054911910228E-6 2.9532164735730683E-5 215-219 0.13502401038823425 9.844054911910228E-6 2.9532164735730683E-5 220-224 0.13983086240172005 9.844054911910228E-6 2.9532164735730683E-5 225-229 0.14440342590830232 9.844054911910228E-6 3.3469786700494775E-5 230-234 0.14903899136632087 9.844054911910228E-6 3.4454192191685797E-5 235-239 0.15357808508620266 9.844054911910228E-6 3.4454192191685797E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TGTTTGC 1027410 0.0 243.18834 3 TTTGCTC 1439710 0.0 242.5664 5 CTGTTTG 1091925 0.0 242.54652 2 GTTTGCT 1483775 0.0 242.51096 4 CCTGTTT 1091060 0.0 240.06087 1 CTGTTCG 212175 0.0 238.98529 2 TGTTTGA 63875 0.0 238.4928 3 TGTTCGC 211045 0.0 237.8486 3 TTTGATC 65885 0.0 237.1237 5 TTGCTCC 1481910 0.0 236.7744 6 GCTCCCC 1825120 0.0 235.09924 8 TGCTCCC 1491915 0.0 235.08107 7 GTTCGCT 341865 0.0 235.0363 4 CCTGTTC 212650 0.0 234.82571 1 GTTTGAT 67455 0.0 233.90161 4 CCCGTTT 453950 0.0 232.50992 1 CTCCCCA 1213075 0.0 231.66774 9 TTTGCTA 58380 0.0 231.3298 5 CCGTTTG 462490 0.0 231.12872 2 CGTTTGC 474140 0.0 224.5157 3 >>END_MODULE