FastQCFastQC Report
Thu 20 Aug 2015
000000000-AHJKW l01n03.33300000033adc.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AHJKW l01n03.33300000033adc.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20316831
Sequences flagged as poor quality0
Sequence length251
%GC58

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCGTTTGCTCCCCTGGCTTTCGCGCCTCAGCGTCAATACCGGTCCAGGA3103821.5277087258342603No Hit
CCTGTTCGCTCCCCACGCTTTCGCGTCTCAGCGTCAGTACCGTCCCAGAG2919841.4371532646995981No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTGTTGGCCCAGAA2128691.0477470625217093No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAGTACCGGACCAGTG1998730.9837803937041166No Hit
CCTGTTTGCTCCCCACGCTTTCGCGTCTCAGCGTCAGTAACGTCCCAGCG1790390.8812348736867478No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAGTACCGGGCCAGTG1780730.8764801951642951No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTGCAGGCCCAGGG1250100.6153026522689489No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTGTTAGCCCAGGG1102470.5426387609366835No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTATCGGCCCAGAG1063810.523610202791961No Hit
CCCGTTTGCTCCCCTGGCTTTCGCGCCTCAGCGTCAATGCCGGTCCAGGA1031960.507933545344744No Hit
CCTGTTCGCTCCCCACGCTTTCGCGTCTCAGCGTCAGTACCGTTCCAGAG1009510.4968835937061248No Hit
CCTGTTTGCTCCCCACGCTTTCGCACATGAGTGTCAGTATTGGGCCAGGA1002160.4932659035259977No Hit
CCCGTTTGCTCCCCTAGCTTTCGCGCCTCAGCGTCAGTGTCGGTCCAGGA975340.4800650258891261No Hit
CCCGTTTGCTCCCCTAGCTTTCGCGCCTCAGCGTCAAGAACAGGCCAGGT911670.44872647707705987No Hit
CCCGTTCGCTCCCCTAGCTTTCGCGCCTCAGCGTCAGTGTCAGCCCAGCA904250.44507433270474117No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTGTCAGCCCAGAG847880.4173288639355222No Hit
CCTGTTTGCTCCCCACGCTTTCGCACATGAGCGTCAGTATTGGTCCAGGA774580.38125040268337124No Hit
CCTGTTTGCTCCCCACGCTTTCGCGTCTCAGCGTCAGTATCCAACCAGGG763480.37578695220726105No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTGAACGTCGGTACTGGTCCAGGA681050.33521467988782305No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAATACCAGTCCAGTG665730.3276741338252998No Hit
CCTGTTTGCTCCCCACGCTGTCGCACCTGAGTGTCAGTATTGGGCCAGGT659810.32476029357137437No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAGTATCGGGCCAGTG659000.32436160934744207No Hit
CCTGTTTGCTCCCCATGCTTTCGTACCTCAGCGTCAGTATTAGGCCAGAT642720.3163485486491471No Hit
CCCGTTTGCTCCCCTGGCTTTCGCTCCTCAGCGTCAGTGTTGGTCCAGGA620050.30519031240649686No Hit
CCCGTTTGCTCCCCTGGCCTTCGTGCCTCAGCGTCAGGAACTGTCCAGAG589330.29006984406180275No Hit
CCTGTTTGCTCCCCACGCTGTCGCGCCTCAGCGTCAGCAACAGCCCAGCA581300.28611745601467076No Hit
CCTGTTTGCTCCCCACGCTGTCGCGCCTCAGTGTCAGCAACGGCCCAGCA567440.279295525960717No Hit
CCCGTTTGCTCCCCTAGCTTTCGCGCCTCAGCGTCAATGCCGGTCCAGGA555710.2735219877548817No Hit
CCCGTTTGCTCCCCTAGCTTTCGCGCCTCAGCGTCAAGAACAGGCCAGGA538180.2648936736245923No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTTACTGCCCAGAG537260.26444084709864446No Hit
CCGGTTTGCTCCCCCAGCTTTCATCCCTCACCGTCGAGCGCGTTCTGGCA537000.2643128743847896No Hit
CCTGTTCGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTACCGTCCCAGCG532140.26192076904119543No Hit
CCTGTTTGCTCCCCACGCTTTCGCGTCTCAGCGTCAGTACCGTCCCAGCG520810.2563441119335983No Hit
CCCGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGGAGATGCCCAGAG518480.25519727953636073No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAATTACGGGCCAGAT518030.2549757883008428No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTACCGTCCCAGCG515920.2539372405076362No Hit
CCTGTTTGCTCCCCACGCTTTCGCGTCTCAGCGTCAGTACCGTCCCAGAG515240.2536025426406313No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAATATGTGTCCAGTG507790.24993563218594475No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTACAGGTCCAGGG503700.2479225229564591No Hit
CCTGTTTGCTCCCCACGCTTTCGCACTTGAGTGTCAGTACTGGACCAGAG503430.2477896282151483No Hit
CCTGTTTGCTCCCCACGCTGTCGCGCCTCAGCGTCAGCAACGGCCCAGCA502240.24720390694788966No Hit
CCTGTTTGCTCCCCACGCTGTCGCGCCTCAGCGTCAGCCACAGCCCAGCG500010.24610629482521165No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAGTACCGATCCAGTG496810.24453124603930604No Hit
CCCGTTTGCTCCCCTGGCTTTCGCTCCTCAGTGTCAGTGTCGGTCCAGGA493550.24292666508866465No Hit
CCCGTTCGCTCCCCTAGCTTTCGCGCCTCAGCGTCAGTGTCGGCCCAGCA486240.23932866301836145No Hit
CCTGTTTGATCCCCACACTTTCGTGCCTCAGCGTCAGTTACAGCCTAGTA479020.2357749591951619No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTGAGCGTCAGTATTGGTCCAGGA475360.2339734971462823No Hit
CCCGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGGAAATGCCCAGAG472830.23272822419992564No Hit
CCCGTTTGCTCCCCTGGCTTTCGCTCCTCAGTGTCAGTGTTGGTCCAGGA465500.22912037807471058No Hit
CCCGTTTGCTCCCCTGGCTTTCGCGCCTCAGCGTCAGTGTCAGCCCAGCA456350.22461672295251162No Hit
CCTGTTTGCTCCCCACGCTTTCGCGTATCAGCGTCAGTATCCAACCAGGG447990.22050190799933317No Hit
CCCGTTTGCTCCCCTGGCTTTCGCTCCTCAGTGTCAATGCCGGTCCAGGA446990.22000970525373767No Hit
CCCGTTTGCTCCCCTGGCTTTCGCTCCTCAGCGTCAGTATTGGTCCAGGA443770.2184248124129201No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGTGTCAGTACCGGGCCAGTG435660.21443304814614048No Hit
CCCGTTTGCTCCCCTGGCTTTCGCTCCTCAGCGTCAATGCCGGTCCAGGA434860.2140392859496641No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTGTTGGCCCAGGA433450.21334528007837442No Hit
CCCGTTTGCTCCCCTGGCCTTCGTGCCTCAGTGTCAGGAACTGTCCAGAG429390.21134693693125664No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTTGTTGCCCAGAG426460.20990478288666178No Hit
CCTGTTTGCTCCCCACGCTTTCGCGTCTCAGCGTCAGTCACCGTCCAGAG425150.20925999728993167No Hit
CCTGTTCGCTCCCCACGCTTTCGCGTCTCAGCGTCAGTACCGTACTAGAG424790.2090828043015173No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTATCGGGCCAGTG420840.20713860345641502No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGTGTCAGTGTTGGCCCAGGA411860.20271862280096734No Hit
CCTGTTTGCTCCCCTAGCTTTCGTGCCTCAGTGTCAGGAGTTTTCCAGGG407570.20060707302236258No Hit
CCCGTTTGCTCCCCTGGCTTTCGTTCCTCAGTGTCAGTTGCAGTCCAGAA402780.1982494218709601No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTACAGGCCCAGGG393690.19377529891349687No Hit
CCTGTTTGCTCCCCCGGCTTTCGTGCCTCAGTGTCAGGTGTTGTCCAGGG392570.19322403183842993No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGAAATGTTCCAGAG385720.18985244303110066No Hit
CCTGTTCGCTCCCCACGCTTTCGCGCCTTAGCGTCAGTACCGTCCCAGCG381180.18761784256609704No Hit
CCTGTTTGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTACCGGCCCAGAG379310.18669742343183346No Hit
CCCGTTCGCTCCCCTGGCTTTCGCGCATCAGCGTCAGTATCGGCCCAGTA373190.1836851426287889No Hit
CCCGTTTGCTCCCCTAGCTTTCGCTCCTCAGCGTCAATGTCGGTCCAGGA368970.18160804704237585No Hit
CCTGTTTGCTCCCCACGCTTTCGCGTCTCAGCGTCAGTCACTGTCCAGAA368380.1813176474224745No Hit
CCGGTTTGCTCCCCCAGCTTTCATCCCTCACCGTCGAGCGCATTCTGGCA355480.1749682320042924No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAATACCGGTCCAGTG352990.17374264716775958No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAATATGTGTCCAGTA351750.17313231576322113No Hit
CCCGTTTGCTACCCTAGCTTTCGCGCCTCAGCGTCAAGAACAGGCCAGGA344180.16940634097906312No Hit
CCCGTTTGCTCCCCTAGCTTTCGCGCCTCAGCGTCAGTAATGGTCCAGGA333830.16431204256214957No Hit
CCCGTTTGCTCCCCTAGCTTTCGCGCCTCAGCGTCAGTATTGGGCCAGAT330050.16245151618379855No Hit
CCCGTTTGCTCCCCTGGCTTTCGCGCCTCAGCGTCAGTGTTGGCCCAGCA325630.1602759800482664No Hit
CCTGTTTGCTCCCCACGCTTTCGCGTCTCAGCGTCAGTTACGTCCCAGCG325130.16002987867546864No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAATACGTGTCCAGTG325040.15998558042836503No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGAACCGGACCAGTA323510.1592325102276039No Hit
CCTGTTTGCTCCCCACGCTTTCGCGTCTCAGCGTCAGTCATCGTCCAGAA317260.15615624306763196No Hit
CCTGTTTGCTCCCCTAGCTTTCGCGCCTCAGCGTCAGGAACCGTCCAGAA313060.15408899153613081No Hit
CCTGTTCGCTCCCCTAGCTTTCGCGCCTGAGCGTCAGGTAAGGCCCAGAG312880.15400039504192362No Hit
CCCGTTCGCTCCCCTAGCTTTCGCGCCTCAGCGTCAAGAATGGGCCAGGT308900.15204142811445348No Hit
CCCGTTTGCTCCCCTAGCTTTCGCGCCTCAGCGTCAAGAGTGGGCCAGGT306840.15102749045852673No Hit
CCCGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGGAATTGCCCAGAG305090.15016613565373457No Hit
CCTGTTCGCTCCCCATGCTTTCGCTCCTCAGCGTCAGTTAATGCCCAGAG303310.14929001476657455No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAGATCTGGACCAGTT298300.14682407901114106No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGTGTCAGTATCAGTCCAGGT295930.1456575585040797No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTGAGCGTCAGTGTCAGCCCAGGG293920.14466823098543272No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAGTATCGAGCCAGTG289050.14227120361438259No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAACAGTCGTCCAGTA289000.1422465934771028No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTGTTGGTCCAGGA288300.14190205155518595No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTGCTGGTCCAGGG287750.1416313400451084No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTATCGGGCCAGTG282330.13896360116398074No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTGCTGGTCCAGGT281220.13841725611636974No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAGTATTGGCCCAGGT276740.13621218781610184No Hit
CCTGTTTGCTCCCCACGCTTTCGCGTCTCAGCGTCAGCAAAGATCCAGCA274660.13518840610526317No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTGTGGTCCAGTT270790.13328358147980854No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTAATGGCCCAGAG265670.1307635034223595No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAATACATGTCCAGTG264550.13021223634729256No Hit
CCCGTTTGCTCCCCTGGCTTTCGTGCCTCAGCGTCAGGAAATGTCCAGAG264320.1300990297158056No Hit
CCTGTTTGCTCCCCTAGCTTTCGTGCCTCAGCGTCGGTATCTGTCCAGAA263300.12959698291529817No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTGAACGTCGGTGCTGGTCCAGGA259810.12787919533316983No Hit
CCTGTTCGCTCCCCTAGCTTTCGCGCCTCAGCGTCAGGTAAGGCCCAGAG259650.12780044289387454No Hit
CCCGTTCGCTCCCCTGGCTTTCGCGCCTCAGCGTCAGTGTCAGCCCAGCA257530.12675697307321204No Hit
CCTGTTCGCTCCCCACGCTTTCGCGTCTCAGCGTCAGTACCGTCCCAGAA250720.12340507237570662No Hit
CCTGTTCGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTACCGTCCCAGAG250670.12338046223842684No Hit
CCCGTTTGCTCCCCTAGCTTTCGCTCATCAGCGTCAATGCCGGTCCAGGA242710.11946252838348657No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTATTGGCCCAGGA241170.11870453615526949No Hit
CCCGTTCGCTCCCCTAGCTTTCGCGCTTCAGCGTCAGTGTCAGCCCAGCA241000.11862086168851825No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTCCGGTCCAGTG240500.11837476031572049No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTGAGCGTCAGTGTTAGCCCAGGG237530.11691291816130184No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAACAATCGTCCAGTA236560.11643548149807417No Hit
CCCGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTCACCGTCCAGAA230660.11353148529906067No Hit
CCTGTTCGCTCCCCTAGCTTTCGCGCCTCAGCGTCAGGTCAGGCCCAGAG226630.11154790823431075No Hit
CCTGTTTGCTCCCCACGCTGTCGCGCCTCAGCGTCAGTACTGGCCCAGTC224100.11030263528795411No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTGTCAGCCCAGGG222930.10972675807560735No Hit
CCCGTTCGCTCCCCTGGCTTTCGCGCCTCAGCGTCAGTGTCGGCCCAGCA222400.10946589062044174No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTGAGCGTCAGTGTTGACCCAGGG220580.10857008162345791No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTGTTATCCCAGGG218040.10731988664964531No Hit
CCCGTTTGCTCCCCTAGCTTTCGCTCCTCAGCGTCAATGCCGGTCCAGGA217430.10701964297483205No Hit
CCCGTTTGCTCCCCTGGCCTTCGTGCCTCAGTGTCAGGAACTGCCCAGAG214890.10576944800101945No Hit
CCTGTTTGCTCCCCTAGCTTTCGTGCCTCAGTGTCAGGAGTTGTCCAGGG213690.10517880470630485No Hit
CCTGTTTGCTCCCCTAGCTTTCGTGCCTCAGTGTCAGGTGTTGTCCAGGG213460.10506559807481788No Hit
CCTGTTTGCTCCCCACGCTGTCGCGCCTCAGCGTCAGCAACGGCCCAGCG212680.10468167993325336No Hit
CCTGTTTGCTCCCCACGCTTTCGCGTCTCAGCGTCAGTCACCGTCCAGAA211860.10427807368186505No Hit
CCCGTTTGCTCCCCTGGCTTTCGCTCCTCAGCGTCAATATTGGTCCAGGA206510.10164478899292906No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTGTTGACCCAGGG205790.1012904030161003No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTTTGC10274100.0243.188343
TTTGCTC14397100.0242.56645
CTGTTTG10919250.0242.546522
GTTTGCT14837750.0242.510964
CCTGTTT10910600.0240.060871
CTGTTCG2121750.0238.985292
TGTTTGA638750.0238.49283
TGTTCGC2110450.0237.84863
TTTGATC658850.0237.12375
TTGCTCC14819100.0236.77446
GCTCCCC18251200.0235.099248
TGCTCCC14919150.0235.081077
GTTCGCT3418650.0235.03634
CCTGTTC2126500.0234.825711
GTTTGAT674550.0233.901614
CCCGTTT4539500.0232.509921
CTCCCCA12130750.0231.667749
TTTGCTA583800.0231.32985
CCGTTTG4624900.0231.128722
CGTTTGC4741400.0224.51573
Produced by FastQC (version 0.11.2)