FastQCFastQC Report
Thu 20 Aug 2015
000000000-AHJKW l01n02.33200000033adf.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AHJKW l01n02.33200000033adf.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20316831
Sequences flagged as poor quality0
Sequence length12
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGCAAGACTCT5898612.9033120371971397No Hit
CGACTGTCTTAA5843332.87610306942062No Hit
TCGTCGATAATC5734722.8226449292214912No Hit
CCATGCGATAAC5713182.812042882081364No Hit
ACTCACGGTATG5599562.756118806126802No Hit
GGTCACTGACAG5592742.7527619834018404No Hit
CCTCTCGTGATC5364362.640352720362738No Hit
AATCTTGCTGCA5345012.6308285972354644No Hit
CAACACGCACGA5316392.616741754656521No Hit
TGCAATGTTGCT5289352.603432592415618No Hit
AATGGAGCATGA5164672.54206475409477No Hit
AGCTTGACAGCT5126312.523183856773726No Hit
TCGGAGTGTTGT5059342.4902210389011947No Hit
ATCGCTCGAGGA5043882.4826115844542884No Hit
CCATAGGGTTCA5011232.4665411648105944No Hit
GTACCTAATTGC4979282.4508152870888185No Hit
GTCTACACACAT4928342.4257424792281825No Hit
GCCTGAATTTAC4897592.410607244801121No Hit
TATCAGGTGTGC4895702.409676981611945No Hit
ATGTGGGACCCA4872972.3984892132045594No Hit
TAATCCACAGCG4854052.3891767372578925No Hit
GCATGGCTCTAA4842942.3837083647543262No Hit
TGTTATCGCACA4665172.2962094826698123No Hit
TGTAATTGTCGC4592092.260239306021692No Hit
TCTTCCGCTACT4459022.1947418866652977No Hit
TCAAGCTCAAGC4386032.158816008264281No Hit
GCTCAGTGCAGA4381902.1567832109249716No Hit
TACTAATCTGCG4330012.1312428104560204No Hit
TAACGCTTGGGT4226072.0800832570788232No Hit
ATTGGGCTAGGC4113742.024794122666079No Hit
GATTGGTTGCAC4113642.02474490239152No Hit
GAAGAAGCGGTA3657821.8003890468941737No Hit
AATCAGTCTCGT2547781.2540243111733322No Hit
AATCCGTACAGC2497491.229271435097334No Hit
ACACCTGGTGAT2003670.9862118752673584No Hit
GGAGACAAGGGA1934220.9520283945857501No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGCAT1950.06.00000054
GGGCTTT953.4301793E-66.00000054
GTCGAGA600.00339670486.00000053
GTTAGCG953.4301793E-66.00000052
CGATTGA600.00339670486.00000051
TAACGAT1202.5698682E-86.00000051
TGTGCAG953.4301793E-66.00000055
TCAGGAG2450.06.00000053
AGTGCGG600.00339670486.00000055
CGTAGGG1900.06.00000052
CAGGCAC1451.9645086E-106.00000054
TATTAGT1202.5698682E-86.00000051
AGTTCAA1950.06.00000054
CTATTTG2350.06.00000055
ACGTCGA1900.06.00000051
GTATAGC953.4301793E-66.00000056
GGTTGCG1950.06.00000055
GACGATA1202.5698682E-86.00000053
TAGGAGG600.00339670486.00000051
GAACACG1202.5698682E-86.00000051
Produced by FastQC (version 0.11.2)