##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename 000000000-AHJKW l01n02.33200000033adf.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 20316831 Sequences flagged as poor quality 0 Sequence length 12 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.65154797025186 32.0 29.0 33.0 16.0 34.0 2 30.035049609853033 32.0 29.0 34.0 16.0 34.0 3 30.143732553566057 32.0 30.0 34.0 16.0 34.0 4 30.25177223751086 33.0 31.0 34.0 16.0 34.0 5 30.267311570392057 33.0 32.0 34.0 16.0 34.0 6 32.550691050193805 37.0 33.0 37.0 16.0 37.0 7 32.21727866909953 35.0 32.0 37.0 16.0 37.0 8 32.073116963959585 35.0 32.0 37.0 16.0 37.0 9 32.1303486257281 34.0 32.0 37.0 16.0 37.0 10 32.05799285331457 35.0 32.0 37.0 16.0 37.0 11 31.842339191579633 34.0 32.0 37.0 16.0 37.0 12 32.1406409789007 35.0 32.0 37.0 16.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3345125664676374 1101 2 0.4153962997070799 1101 3 0.39177562806386135 1101 4 0.49684560529310673 1101 5 0.4097880481663374 1101 6 0.3981725802935898 1101 7 0.435697066423522 1101 8 0.4881022807514128 1101 9 0.4459846494359425 1101 10 0.5267941325700605 1101 11 0.4238346975579681 1101 12 0.35863774548236904 1102 1 0.2765778974774378 1102 2 0.35731420774739675 1102 3 0.3222387486356517 1102 4 0.2934422774879444 1102 5 0.3348258968595914 1102 6 0.30296139736291394 1102 7 0.2742540974551275 1102 8 0.2837104610886598 1102 9 0.336012029927808 1102 10 0.39477305870701684 1102 11 0.43098968243696234 1102 12 0.22174675650267517 1103 1 0.474102081040062 1103 2 0.31154081839850534 1103 3 0.34285309294685007 1103 4 0.45121419092598103 1103 5 0.40184151712941585 1103 6 0.5068470943221115 1103 7 0.403589287327911 1103 8 0.3778685286325256 1103 9 0.40167092657291903 1103 10 0.38165475562551165 1103 11 0.5324527658310778 1103 12 0.2765174750915165 2112 1 0.40326340165178465 2112 2 0.3108528301104272 2112 3 0.18455367527398892 2112 4 0.32945062067750897 2112 5 0.3065310022160972 2112 6 0.33858892745498537 2112 7 0.20235679559228004 2112 8 0.21000019967597439 2112 9 0.2095362274642909 2112 10 0.2621935772523827 2112 11 0.2666037335827305 2112 12 0.07838692140256143 1113 1 0.3152516098388567 1113 2 0.20389265634778297 1113 3 0.24768041667580576 1113 4 0.05834629882946274 1113 5 0.16217407568643338 1113 6 0.25650552006240446 1113 7 -0.04586976786666952 1113 8 0.2773981047937113 1113 9 0.1422352775031328 1113 10 0.1543588383400376 1113 11 0.3117133757842261 1113 12 0.14097349437503226 2113 1 0.3231317017840709 2113 2 0.12021475602933762 2113 3 0.19180145324205355 2113 4 0.3414664299683672 2113 5 0.22492849913339796 2113 6 0.27825412344765255 2113 7 0.38691333109859016 2113 8 0.41116347678049436 2113 9 0.29869928897635134 2113 10 0.21146602489418598 2113 11 0.3627062615405414 2113 12 0.20227967139521752 1112 1 0.28469402541170297 1112 2 0.174368910792559 1112 3 0.2721944394850446 1112 4 0.09927410615767229 1112 5 0.13222471773047317 1112 6 0.13042859485702252 1112 7 0.3026344687934497 1112 8 0.32293018997202694 1112 9 0.09902230165279491 1112 10 0.1663606728633127 1112 11 0.279675542450601 1112 12 -0.071129917947367 2114 1 0.37861203269659427 2114 2 0.2376078322905606 2114 3 0.23696226614397276 2114 4 0.27511569246512835 2114 5 0.11081163967002894 2114 6 0.1973485844460754 2114 7 0.3545545288280394 2114 8 0.39973392430565013 2114 9 0.2239512802538357 2114 10 0.258164263475507 2114 11 0.3003433230316297 2114 12 -0.04408357284354736 1114 1 0.350565006205823 1114 2 0.3075718724932024 1114 3 0.07857031020281369 1114 4 0.24995100250119506 1114 5 0.2230807809387727 1114 6 0.20653273373716985 1114 7 0.3388752513843656 1114 8 0.2810110333433187 1114 9 0.23950372111892193 1114 10 0.27648008666014334 1114 11 0.2642744278621372 1114 12 0.2679176368434 1109 1 0.4999661175936474 1109 2 0.25395019322449386 1109 3 0.23171007383983167 1109 4 0.2432859356588395 1109 5 0.19150929331721045 1109 6 0.29616498745922115 1109 7 0.26284685668984764 1109 8 0.25476744951579633 1109 9 0.28381758077636476 1109 10 0.35338487864447643 1109 11 0.3941546226313015 1109 12 0.29994938652227887 1108 1 0.6063089540917659 1108 2 0.41450418072706086 1108 3 0.41518856902789025 1108 4 0.4138040664853513 1108 5 0.3904859183843534 1108 6 0.22729489401198322 1108 7 0.4586963335297085 1108 8 0.3981581069494453 1108 9 0.44477432298477737 1108 10 0.4500829191717628 1108 11 0.5367462871872313 1108 12 0.4664945565924157 1111 1 0.5600497335717094 1111 2 0.2767590815164489 1111 3 0.34750781627575833 1111 4 0.43096301584806795 1111 5 0.3390284286436156 1111 6 0.2618764470477899 1111 7 0.41206013192559254 1111 8 0.4747294552548098 1111 9 0.35156748825785655 1111 10 0.4682857540867005 1111 11 0.5304767795547853 1111 12 0.4626811763628069 1110 1 0.6360478671803449 1110 2 0.585544693814473 1110 3 0.3475211902456934 1110 4 0.4945110122269831 1110 5 0.3810007866058598 1110 6 0.04372324212351231 1110 7 0.25204686518222275 1110 8 0.3492813256083238 1110 9 0.4152053991501532 1110 10 0.40063412483866045 1110 11 0.4564097929523534 1110 12 0.5155962349408298 1105 1 0.6200871413665645 1105 2 0.4427273861144734 1105 3 0.37447117597740487 1105 4 0.30947315432881695 1105 5 0.3247335604903796 1105 6 -0.07128906937876422 1105 7 0.24889100503214223 1105 8 0.4437118920929777 1105 9 0.4312285150975441 1105 10 0.3709950323991009 1105 11 0.45875428546670705 1105 12 0.5243043147770194 1104 1 -1.1525414224821589 1104 2 -0.6332400093123169 1104 3 -0.2983213031713987 1104 4 -0.29452417844154155 1104 5 -0.24929055468020778 1104 6 0.004076113395584002 1104 7 0.004284979497221286 1104 8 -0.38001628079978644 1104 9 -0.23358857159519886 1104 10 0.04706749666507193 1104 11 -0.09402992162597101 1104 12 -0.05521277058352325 1107 1 -0.3590445863859024 1107 2 -0.3926905154876863 1107 3 -0.2302342133432589 1107 4 -0.2248817820188158 1107 5 -0.12011855131782667 1107 6 -0.021212961845506584 1107 7 -0.0498035127869656 1107 8 -0.3672320183693536 1107 9 -0.055492092759202194 1107 10 0.13715632403057754 1107 11 -0.3499214028752071 1107 12 -0.07737247946330683 1106 1 -0.16144509006892704 1106 2 -0.3372216085448123 1106 3 -0.2607901843130058 1106 4 -0.42810555612745205 1106 5 -0.3750795435607941 1106 6 -0.2008662082375352 1106 7 -0.28073600835478985 1106 8 -0.7991800386093715 1106 9 -0.4683362381560876 1106 10 -0.6996939442871195 1106 11 -0.7743877737622817 1106 12 -0.5075989643121908 2103 1 -0.5657664327106389 2103 2 -0.1722288892722652 2103 3 -0.2882317719901337 2103 4 -0.3296805114243142 2103 5 -0.40381836245054714 2103 6 -0.16689303928059473 2103 7 -0.253727235711974 2103 8 -0.6840756152825129 2103 9 -0.3847868360251532 2103 10 -0.7035421241788491 2103 11 -0.6098014896809971 2103 12 -0.4655146518827742 2102 1 -0.1369199183486387 2102 2 -0.15740092730318622 2102 3 -0.2814477877580899 2102 4 -0.2195437396571016 2102 5 -0.4036884342218663 2102 6 -0.12371146574118086 2102 7 -0.10648878085029168 2102 8 -0.3466660135167068 2102 9 -0.18350862512401633 2102 10 -0.43822708677795674 2102 11 -0.2405090455115655 2102 12 -0.2807776332914145 2101 1 -0.3875982003956757 2101 2 -0.1483351176760337 2101 3 -0.16470475413122188 2101 4 -0.25305084632168473 2101 5 -0.3581055342354702 2101 6 -6.053671175578756E-4 2101 7 -0.30710345954338436 2101 8 -0.09073281621420293 2101 9 -0.005950607961629828 2101 10 -0.2947077066061361 2101 11 -0.2609440473262943 2101 12 -0.024512498305583108 2107 1 -0.415482491952222 2107 2 -0.42125907064079016 2107 3 -0.42570543442291964 2107 4 -0.5485347937245315 2107 5 -0.33530067119096074 2107 6 -0.43862834457088695 2107 7 -0.34493644861077755 2107 8 -0.3991050764780866 2107 9 -0.31369125828764055 2107 10 -0.49710678096063177 2107 11 -0.44561313231950805 2107 12 -0.31129888474086087 2106 1 -0.5931797829678409 2106 2 -0.2749200281802011 2106 3 -0.3008292523579321 2106 4 -0.16967659716040018 2106 5 -0.1981179122792227 2106 6 -0.7419205508258386 2106 7 -0.3881002587134823 2106 8 -0.6308329693655494 2106 9 -0.48548193534737294 2106 10 -0.4350794914031262 2106 11 -0.5347382433453731 2106 12 -0.46737728940517087 2105 1 -0.23777435405242997 2105 2 -0.19886928396817183 2105 3 -0.4734287308541596 2105 4 -0.2616328037882738 2105 5 -0.18589939842591363 2105 6 -0.7663613481746694 2105 7 -0.3107028427937095 2105 8 -0.2999622161527533 2105 9 -0.281883778728794 2105 10 -0.5124092324299951 2105 11 -0.4811148436198387 2105 12 -0.5364461892508281 2104 1 -0.3790908906249584 2104 2 -0.38363108601462415 2104 3 -0.3328871034597647 2104 4 -0.4154716718820204 2104 5 -0.17029124627969594 2104 6 -0.7265316020243304 2104 7 -0.39803988595883055 2104 8 -0.20250875067515395 2104 9 -0.321027002350732 2104 10 -0.40153070331174234 2104 11 -0.29214205875584653 2104 12 -0.4342725229987252 2111 1 -0.3379650030919521 2111 2 -0.36550984829719724 2111 3 -0.3079662550257254 2111 4 -0.3286050222242416 2111 5 -0.26493138858685583 2111 6 -0.027774781635713452 2111 7 -0.3277812598468728 2111 8 -0.22859455225532344 2111 9 -0.24836703620525213 2111 10 -0.08243831988873751 2111 11 -0.2711295860580307 2111 12 -0.3847566714756354 2110 1 -0.5322591544790498 2110 2 -0.23419044155415847 2110 3 -0.0991790007521054 2110 4 -0.2731123824343591 2110 5 -0.16830245377235542 2110 6 0.08758891011211034 2110 7 -0.27243847474615634 2110 8 -0.1933806324282017 2110 9 -0.2757574860237746 2110 10 -0.03857738308771275 2110 11 -0.1984895379217413 2110 12 -0.253594520313154 2109 1 -0.6126166387913976 2109 2 -0.393388915449556 2109 3 -0.37671466336653125 2109 4 -0.4121224804239212 2109 5 -0.5559827538682072 2109 6 -0.24388391383457275 2109 7 -0.9039454189907659 2109 8 -0.4245077926866436 2109 9 -0.8241430655958233 2109 10 -0.6213120800371321 2109 11 -0.6893212340310875 2109 12 -0.11031946278195903 2108 1 -0.19148617002607082 2108 2 -0.29935997761295496 2108 3 -0.14458840109021054 2108 4 -0.3282010432257785 2108 5 -0.1440373601023559 2108 6 -0.006685497467103119 2108 7 -0.3480276439852936 2108 8 0.07422834406860801 2108 9 -0.24119447501220037 2108 10 -0.135227087255398 2108 11 -0.3069932610365278 2108 12 0.20878265930802087 >>END_MODULE >>Per sequence quality scores pass #Quality Count 14 33.0 15 2311.0 16 62391.0 17 174015.0 18 301042.0 19 436605.0 20 455401.0 21 527971.0 22 531918.0 23 504516.0 24 489663.0 25 469266.0 26 453932.0 27 453451.0 28 461463.0 29 510441.0 30 565549.0 31 765431.0 32 1107021.0 33 2271163.0 34 4067691.0 35 5086626.0 36 618931.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 22.753778796136476 24.848032080661568 36.22646945964097 16.171719663560992 2 22.88742879671681 27.603356429127967 15.37360405141944 34.135610722735784 3 13.127372078844383 30.223168170272224 38.11822326031062 18.531236490572766 4 14.962968388131003 9.200622872730497 37.86746564953954 37.96894308959896 5 25.03277824345629 42.82553922043941 13.244438231751705 18.897244304352597 6 34.54513819331067 14.514828346745038 20.087907414690186 30.852126045254106 7 39.312572910607955 19.288766048208995 29.0978794872094 12.300781553973648 8 24.343289561250963 15.605735953604183 26.885265718851524 33.16570876629333 9 14.632478854600897 29.39212813258131 38.69217103789464 17.283221974923155 10 27.083846249636384 24.87740459510662 27.871124579966462 20.167624575290535 11 34.395216458708546 22.17003232443091 12.952689324432537 30.482061892428007 12 11.722512236283306 33.25944877919199 23.822027165555497 31.19601181896921 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 838.0 1 838.0 2 838.0 3 838.0 4 3459.0 5 6080.0 6 6080.0 7 6080.0 8 6080.0 9 6080.0 10 6080.0 11 6080.0 12 6080.0 13 16136.0 14 26192.0 15 26192.0 16 26192.0 17 26192.0 18 26192.0 19 26192.0 20 26192.0 21 61774.5 22 97357.0 23 97357.0 24 97357.0 25 97357.0 26 97357.0 27 97357.0 28 97357.0 29 264092.5 30 430828.0 31 430828.0 32 430828.0 33 430828.0 34 430828.0 35 430828.0 36 430828.0 37 430828.0 38 3568656.5 39 6706485.0 40 6706485.0 41 6706485.0 42 6706485.0 43 6706485.0 44 6706485.0 45 6706485.0 46 7514408.0 47 8322331.0 48 8322331.0 49 8322331.0 50 8322331.0 51 8322331.0 52 8322331.0 53 8322331.0 54 6354132.0 55 4385933.0 56 4385933.0 57 4385933.0 58 4385933.0 59 4385933.0 60 4385933.0 61 4385933.0 62 4385933.0 63 2332258.5 64 278584.0 65 278584.0 66 278584.0 67 278584.0 68 278584.0 69 278584.0 70 278584.0 71 164405.0 72 50226.0 73 50226.0 74 50226.0 75 50226.0 76 50226.0 77 50226.0 78 50226.0 79 29588.0 80 8950.0 81 8950.0 82 8950.0 83 8950.0 84 8950.0 85 8950.0 86 8950.0 87 8950.0 88 5238.5 89 1527.0 90 1527.0 91 1527.0 92 1527.0 93 1527.0 94 1527.0 95 1527.0 96 1513.5 97 1500.0 98 1500.0 99 1500.0 100 1500.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 2.1164718060606992E-4 2 4.922027455955114E-6 3 0.0 4 0.0 5 4.922027455955114E-6 6 4.922027455955114E-6 7 0.0 8 0.0 9 0.0 10 4.922027455955114E-6 11 0.0 12 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 12 2.0316831E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 3.3408066480245404 #Duplication Level Percentage of deduplicated Percentage of total 1 65.96864028111368 2.203884720122841 2 13.246988556098113 0.8851125486903517 3 5.764108775539728 0.5777031875177916 4 3.2935592647461625 0.44012578749307196 5 2.288414071287991 0.38225744713959126 6 1.278506357938827 0.2562745524086205 7 1.1108362575990312 0.25977624079774814 8 0.8518409863131226 0.22766688241077296 9 0.6345791451334415 0.19080056040835783 >10 4.619626110315937 2.9596840346352424 >50 0.4756917429761701 1.0931679354355484 >100 0.31532178678537426 2.223118417532971 >500 0.07605964367495924 1.8007704073147668 >1k 0.06835410865099047 4.126019518553916 >5k 0.0020513875902483485 0.42678858971794753 >10k+ 0.005421524236305707 81.94684916982054 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source TGGCAAGACTCT 589861 2.9033120371971397 No Hit CGACTGTCTTAA 584333 2.87610306942062 No Hit TCGTCGATAATC 573472 2.8226449292214912 No Hit CCATGCGATAAC 571318 2.812042882081364 No Hit ACTCACGGTATG 559956 2.756118806126802 No Hit GGTCACTGACAG 559274 2.7527619834018404 No Hit CCTCTCGTGATC 536436 2.640352720362738 No Hit AATCTTGCTGCA 534501 2.6308285972354644 No Hit CAACACGCACGA 531639 2.616741754656521 No Hit TGCAATGTTGCT 528935 2.603432592415618 No Hit AATGGAGCATGA 516467 2.54206475409477 No Hit AGCTTGACAGCT 512631 2.523183856773726 No Hit TCGGAGTGTTGT 505934 2.4902210389011947 No Hit ATCGCTCGAGGA 504388 2.4826115844542884 No Hit CCATAGGGTTCA 501123 2.4665411648105944 No Hit GTACCTAATTGC 497928 2.4508152870888185 No Hit GTCTACACACAT 492834 2.4257424792281825 No Hit GCCTGAATTTAC 489759 2.410607244801121 No Hit TATCAGGTGTGC 489570 2.409676981611945 No Hit ATGTGGGACCCA 487297 2.3984892132045594 No Hit TAATCCACAGCG 485405 2.3891767372578925 No Hit GCATGGCTCTAA 484294 2.3837083647543262 No Hit TGTTATCGCACA 466517 2.2962094826698123 No Hit TGTAATTGTCGC 459209 2.260239306021692 No Hit TCTTCCGCTACT 445902 2.1947418866652977 No Hit TCAAGCTCAAGC 438603 2.158816008264281 No Hit GCTCAGTGCAGA 438190 2.1567832109249716 No Hit TACTAATCTGCG 433001 2.1312428104560204 No Hit TAACGCTTGGGT 422607 2.0800832570788232 No Hit ATTGGGCTAGGC 411374 2.024794122666079 No Hit GATTGGTTGCAC 411364 2.02474490239152 No Hit GAAGAAGCGGTA 365782 1.8003890468941737 No Hit AATCAGTCTCGT 254778 1.2540243111733322 No Hit AATCCGTACAGC 249749 1.229271435097334 No Hit ACACCTGGTGAT 200367 0.9862118752673584 No Hit GGAGACAAGGGA 193422 0.9520283945857501 No Hit >>END_MODULE >>Adapter Content warn >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGGGCAT 195 0.0 6.0000005 4 GGGCTTT 95 3.4301793E-6 6.0000005 4 GTCGAGA 60 0.0033967048 6.0000005 3 GTTAGCG 95 3.4301793E-6 6.0000005 2 CGATTGA 60 0.0033967048 6.0000005 1 TAACGAT 120 2.5698682E-8 6.0000005 1 TGTGCAG 95 3.4301793E-6 6.0000005 5 TCAGGAG 245 0.0 6.0000005 3 AGTGCGG 60 0.0033967048 6.0000005 5 CGTAGGG 190 0.0 6.0000005 2 CAGGCAC 145 1.9645086E-10 6.0000005 4 TATTAGT 120 2.5698682E-8 6.0000005 1 AGTTCAA 195 0.0 6.0000005 4 CTATTTG 235 0.0 6.0000005 5 ACGTCGA 190 0.0 6.0000005 1 GTATAGC 95 3.4301793E-6 6.0000005 6 GGTTGCG 195 0.0 6.0000005 5 GACGATA 120 2.5698682E-8 6.0000005 3 TAGGAGG 60 0.0033967048 6.0000005 1 GAACACG 120 2.5698682E-8 6.0000005 1 >>END_MODULE