##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename 000000000-AHJKW l01n01.33100000033ad2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 20316831 Sequences flagged as poor quality 0 Sequence length 251 %GC 56 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.20564496500463 33.0 32.0 33.0 32.0 34.0 2 32.37859221253551 33.0 32.0 33.0 32.0 34.0 3 32.58290670429852 33.0 32.0 33.0 32.0 34.0 4 32.18055330577884 33.0 32.0 33.0 32.0 34.0 5 31.318964655462263 33.0 32.0 33.0 29.0 34.0 6 33.67821438294191 35.0 33.0 37.0 30.0 37.0 7 34.37074310457177 37.0 33.0 37.0 32.0 37.0 8 32.34200200808876 33.0 32.0 34.0 31.0 35.0 9 32.57416375614878 33.0 32.0 34.0 31.0 37.0 10-14 33.772340036691745 34.8 33.2 36.4 28.6 37.0 15-19 37.02489142130482 38.0 37.2 38.0 35.2 38.0 20-24 36.2965291191328 38.2 36.8 38.2 33.2 38.2 25-29 35.79430931920435 38.0 36.0 38.2 29.8 38.2 30-34 37.68064664218549 39.0 37.6 39.0 36.0 39.0 35-39 37.266035298516776 38.4 37.4 38.4 35.6 38.4 40-44 37.033508139138426 38.4 37.6 38.6 34.8 38.8 45-49 35.770206603579076 38.0 36.0 38.0 32.2 38.2 50-54 36.832682902171115 38.0 37.0 38.2 34.6 38.8 55-59 34.55519819995549 37.6 35.2 38.0 20.8 38.2 60-64 35.57176646298825 38.2 36.2 39.0 26.2 39.0 65-69 35.25380919888539 37.8 35.8 38.6 25.4 39.0 70-74 35.856181940972974 38.0 36.0 38.8 29.4 39.0 75-79 36.58293859903644 38.6 37.0 39.0 32.2 39.0 80-84 35.05638502382582 38.0 35.4 38.4 25.0 38.8 85-89 35.366987548402605 37.4 35.2 38.6 28.0 38.8 90-94 35.291743018387066 38.4 35.2 39.0 24.8 39.0 95-99 34.05749786470143 37.4 33.6 38.2 20.2 39.0 100-104 34.4139431489094 37.6 34.4 38.8 21.0 39.0 105-109 34.204407586990314 37.2 33.6 38.6 22.2 39.0 110-114 35.23047660336398 38.2 35.6 39.0 24.8 39.0 115-119 34.0642373311074 37.2 33.4 38.4 21.0 39.0 120-124 34.20180389353044 37.6 34.2 39.0 18.2 39.0 125-129 33.16324230880298 37.0 32.4 38.4 16.8 39.0 130-134 33.81296219868148 37.0 32.8 38.0 22.2 39.0 135-139 32.106419529699295 37.0 29.8 38.0 13.4 38.8 140-144 33.745611714740356 37.0 33.0 38.0 21.0 38.8 145-149 33.788761495333596 37.0 34.0 38.2 18.8 38.8 150-154 33.38847307436873 36.8 32.2 37.8 20.6 38.6 155-159 33.27995929089532 36.6 32.6 37.8 18.8 38.4 160-164 32.622284036324366 36.6 31.8 37.2 16.4 38.0 165-169 32.774816564650266 36.2 32.0 37.4 17.8 38.0 170-174 31.524574132648933 36.4 28.0 37.0 15.8 38.0 175-179 31.45486127241005 36.6 28.8 37.0 14.0 38.0 180-184 30.35552593807568 34.0 24.8 36.6 15.4 37.2 185-189 31.868110021685965 35.8 30.0 37.0 15.8 37.4 190-194 31.309327040225913 35.0 28.8 37.0 15.4 37.0 195-199 28.89215039491149 32.4 24.2 35.0 12.6 37.0 200-204 26.711911016043793 28.4 18.4 35.8 10.0 37.0 205-209 29.24095321755642 31.2 24.4 37.0 13.8 37.0 210-214 28.715839040055016 32.8 20.0 37.0 12.6 37.0 215-219 29.528305856361165 33.8 24.4 37.0 13.2 37.0 220-224 29.797678397777684 33.8 24.4 37.0 13.4 37.0 225-229 28.238670647011826 32.6 19.8 36.6 13.2 37.0 230-234 26.424494922461086 29.0 19.6 34.6 12.2 35.8 235-239 26.03934263173228 29.0 17.4 34.2 12.4 36.0 240-244 27.39527333765783 30.4 22.2 35.0 12.2 37.0 245-249 24.714791002592875 26.0 15.8 32.4 12.0 36.2 250-251 21.11098817527202 19.0 13.0 28.0 12.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.1529228272624863 1101 2 0.1199594952141041 1101 3 0.13571712854596996 1101 4 0.19476142438023913 1101 5 0.36928372008165056 1101 6 0.214554005440597 1101 7 0.30450531172524364 1101 8 0.3842117535112948 1101 9 0.20931289900529748 1101 10-14 0.3058092832857753 1101 15-19 0.26072816575376834 1101 20-24 0.41951864284108353 1101 25-29 0.4286664919580545 1101 30-34 0.2880661170080998 1101 35-39 0.17168613371702435 1101 40-44 0.28068712388341766 1101 45-49 0.27244671223787265 1101 50-54 0.22865661442710206 1101 55-59 0.3943536007232993 1101 60-64 0.3260226444179182 1101 65-69 0.43288989409119694 1101 70-74 0.3454413723616341 1101 75-79 0.2906097084456647 1101 80-84 0.41106680576388754 1101 85-89 0.7227414094724054 1101 90-94 0.22624309280328703 1101 95-99 0.4075582613709372 1101 100-104 0.4944422357057974 1101 105-109 0.4505008589702868 1101 110-114 0.29274814322354814 1101 115-119 0.46556304979061736 1101 120-124 0.4428048516287575 1101 125-129 0.6781096756201279 1101 130-134 0.5713255959017616 1101 135-139 0.7228835750268985 1101 140-144 0.3722325549195915 1101 145-149 0.6422360748222076 1101 150-154 0.32385762121721484 1101 155-159 0.44919277839031224 1101 160-164 0.4190700941244003 1101 165-169 0.5201556321612983 1101 170-174 1.2298823546060937 1101 175-179 0.7185436989045293 1101 180-184 0.19932338556171203 1101 185-189 0.5537570262195359 1101 190-194 1.2287955044340535 1101 195-199 1.1821162766634181 1101 200-204 3.2855397993118487 1101 205-209 2.631699917294249 1101 210-214 1.0427341514198787 1101 215-219 0.7991164552082317 1101 220-224 0.4403860074394075 1101 225-229 0.4628492517912157 1101 230-234 0.4639267776499416 1101 235-239 0.27037640935762397 1101 240-244 0.55767049728831 1101 245-249 -0.20207760023630783 1101 250-251 -0.4221827569128358 1102 1 0.21164689239645895 1102 2 0.10652277778054042 1102 3 0.10902852581057942 1102 4 0.15259752530137405 1102 5 0.30247646797587535 1102 6 0.07762456894482028 1102 7 0.2650402943559129 1102 8 0.35715960637109134 1102 9 0.1325494310163009 1102 10-14 0.30203110078338824 1102 15-19 0.26642102784210664 1102 20-24 0.3504161342698069 1102 25-29 0.3390371867538491 1102 30-34 0.24010298922524242 1102 35-39 0.1652978557584106 1102 40-44 0.30782845373236967 1102 45-49 0.3480152166233168 1102 50-54 0.3152827636613935 1102 55-59 0.5939556096409291 1102 60-64 0.3372589097037917 1102 65-69 0.4767387753451615 1102 70-74 0.3718919774943288 1102 75-79 0.31813723647800884 1102 80-84 0.45781678518758184 1102 85-89 0.7485948484109954 1102 90-94 0.30189050860749944 1102 95-99 0.41283727042052476 1102 100-104 0.6390361166956708 1102 105-109 0.5449904379192816 1102 110-114 0.4552144229549029 1102 115-119 0.728579701155347 1102 120-124 0.5957961613226317 1102 125-129 0.8103081573564452 1102 130-134 0.7024030988526206 1102 135-139 0.8805159964829699 1102 140-144 0.4640603291930745 1102 145-149 0.6924994920820069 1102 150-154 0.6610134303694437 1102 155-159 0.6897960852368712 1102 160-164 0.5949001924790736 1102 165-169 0.7321393056755952 1102 170-174 1.460737869451112 1102 175-179 0.5503836557792177 1102 180-184 0.16932462868661702 1102 185-189 0.620829831316577 1102 190-194 1.381607969499541 1102 195-199 1.5134227914788454 1102 200-204 3.4615420261696954 1102 205-209 2.851047716134463 1102 210-214 1.3692850077353818 1102 215-219 1.0953327077550554 1102 220-224 0.9932579068629934 1102 225-229 1.1429393353858934 1102 230-234 1.1648806768537945 1102 235-239 0.7733872872995704 1102 240-244 0.9156728008275294 1102 245-249 0.23273976212017544 1102 250-251 0.24330139166884734 1103 1 0.18872834362048252 1103 2 0.11142681024294632 1103 3 0.09859128697073061 1103 4 0.025237096244971724 1103 5 0.3256609859044062 1103 6 0.2271690256963126 1103 7 0.2796401770333574 1103 8 0.3458342318487695 1103 9 0.19719590303505186 1103 10-14 0.22561181527031948 1103 15-19 0.2697593271089005 1103 20-24 0.3722350639441103 1103 25-29 0.3408329384195312 1103 30-34 0.2297503733917594 1103 35-39 0.1459755187095837 1103 40-44 0.31047676149445635 1103 45-49 0.3439303603541788 1103 50-54 0.28528516325977193 1103 55-59 0.44822170976146936 1103 60-64 0.3488226691079035 1103 65-69 0.5679536065090929 1103 70-74 0.3258427594602722 1103 75-79 0.2979608641576874 1103 80-84 0.39833165344122534 1103 85-89 0.5393106729148727 1103 90-94 0.4573271590415615 1103 95-99 0.47605705997798253 1103 100-104 0.47880131205513266 1103 105-109 0.4030555369024782 1103 110-114 0.1699686761426591 1103 115-119 -0.5578301192156019 1103 120-124 0.279772547178041 1103 125-129 0.5843272001419706 1103 130-134 0.7055738154124143 1103 135-139 0.7413046235421348 1103 140-144 0.5541970130480252 1103 145-149 0.7140841052956475 1103 150-154 0.39141339421149013 1103 155-159 0.36056202139454996 1103 160-164 0.2161493009052151 1103 165-169 0.8077548025081711 1103 170-174 1.4589155484935894 1103 175-179 0.16528328780299617 1103 180-184 0.3290489392296543 1103 185-189 0.5958680977693476 1103 190-194 1.3906175102095446 1103 195-199 1.4894904392805692 1103 200-204 3.294104293740215 1103 205-209 2.97849980952736 1103 210-214 1.2452861477515 1103 215-219 1.0839422129783394 1103 220-224 0.7498104706188613 1103 225-229 1.0305034183633772 1103 230-234 0.7983433272842326 1103 235-239 0.36489724408504287 1103 240-244 1.105368798469673 1103 245-249 0.057760327128889344 1103 250-251 -0.14489481943610727 2112 1 0.20870110827091537 2112 2 0.10987065883759328 2112 3 0.11529704904389604 2112 4 0.1479845492918841 2112 5 0.33532812081447005 2112 6 0.23022891904010123 2112 7 0.27715649442908585 2112 8 0.34818012738663384 2112 9 0.42439043741331517 2112 10-14 0.3076678927978307 2112 15-19 0.25120176950026263 2112 20-24 0.26036667885714593 2112 25-29 0.1199834191789364 2112 30-34 0.15291032509821179 2112 35-39 0.15357163348792824 2112 40-44 0.33829957485298223 2112 45-49 0.37244388783706484 2112 50-54 0.3274642826655736 2112 55-59 0.571825631021369 2112 60-64 0.4280481902087132 2112 65-69 0.516839358428058 2112 70-74 0.3286036330868001 2112 75-79 0.32505725719811096 2112 80-84 0.36235004617666533 2112 85-89 0.5092787730203696 2112 90-94 0.4469758567207762 2112 95-99 0.67915467554635 2112 100-104 0.6137258062219431 2112 105-109 0.49033608897347847 2112 110-114 0.3700183597732831 2112 115-119 0.2717416559076682 2112 120-124 0.23211684003786104 2112 125-129 0.7489998123815127 2112 130-134 0.6945819673132618 2112 135-139 0.6306334574444676 2112 140-144 0.38121261028690867 2112 145-149 0.6064622607193257 2112 150-154 0.7799371819053533 2112 155-159 0.7668679915787067 2112 160-164 0.5922802815226618 2112 165-169 0.9491930562527884 2112 170-174 1.5100888849363976 2112 175-179 0.8212267303950433 2112 180-184 0.03453278470561827 2112 185-189 0.5546599663885274 2112 190-194 1.2966177731477657 2112 195-199 1.61806893015968 2112 200-204 3.121022325813801 2112 205-209 2.785155803648994 2112 210-214 0.805878546303326 2112 215-219 0.453024753849931 2112 220-224 0.8094226565783131 2112 225-229 1.0691949061130295 2112 230-234 0.8658555663319376 2112 235-239 0.8214712041804866 2112 240-244 1.1642250751711956 2112 245-249 0.3619149838975986 2112 250-251 -0.01955020736401991 1113 1 0.1509345687226471 1113 2 0.037559748537105975 1113 3 0.05268731661731607 1113 4 0.1361339301088833 1113 5 0.22736205195972303 1113 6 0.23064833476370694 1113 7 0.2603356192947004 1113 8 0.35532081366728363 1113 9 0.3954250410299238 1113 10-14 0.2441659981044495 1113 15-19 0.2296422016485451 1113 20-24 0.31625371616001985 1113 25-29 0.2792032015230035 1113 30-34 0.18016870405168106 1113 35-39 0.14372642266578595 1113 40-44 0.275835639196103 1113 45-49 0.2578691680849232 1113 50-54 0.2654780817361768 1113 55-59 0.48864711328526766 1113 60-64 0.34459062953999364 1113 65-69 0.4521974494866967 1113 70-74 0.33194771428602365 1113 75-79 0.30672570471729443 1113 80-84 0.4690949152078474 1113 85-89 0.3928076079009841 1113 90-94 0.3119687495524204 1113 95-99 0.4547394891509242 1113 100-104 0.3934306505296945 1113 105-109 0.3662113021606004 1113 110-114 0.4138418482869568 1113 115-119 0.7668856576019536 1113 120-124 0.5789891630365176 1113 125-129 0.8722460962980705 1113 130-134 0.47238812557815635 1113 135-139 0.8037443449525057 1113 140-144 0.5894222770799047 1113 145-149 0.5588707959048733 1113 150-154 0.5057916773243605 1113 155-159 0.4005533272498383 1113 160-164 0.06808200319821367 1113 165-169 0.38125223709444356 1113 170-174 1.2486366079087468 1113 175-179 0.5482195834815009 1113 180-184 0.15387435153027695 1113 185-189 0.6395037108253447 1113 190-194 1.0885183746825362 1113 195-199 1.4609753836397203 1113 200-204 -3.5841843257795105 1113 205-209 -2.994702756182395 1113 210-214 0.7053207021666381 1113 215-219 0.5477180817771661 1113 220-224 0.7312435348851487 1113 225-229 0.7549590577915879 1113 230-234 0.9864981687465786 1113 235-239 0.7592770497617245 1113 240-244 0.8423884142672158 1113 245-249 0.21059019944569002 1113 250-251 -0.17741083119137002 2113 1 0.17834821886160057 2113 2 0.07046353870763511 2113 3 0.08014745870637086 2113 4 0.11842240626378953 2113 5 0.28709531712582503 2113 6 0.1809711897963453 2113 7 0.27670632110242366 2113 8 0.3645783694724969 2113 9 0.3491861745296916 2113 10-14 0.16270507045965843 2113 15-19 0.1551450812237931 2113 20-24 0.3405933483191603 2113 25-29 0.4232766242906578 2113 30-34 0.24818564427144452 2113 35-39 0.1399807003573983 2113 40-44 0.2723052088673299 2113 45-49 0.3249837643454683 2113 50-54 0.24741201096070853 2113 55-59 0.41052293543680207 2113 60-64 0.2384397886560734 2113 65-69 0.3329893869011471 2113 70-74 0.3498314220796459 2113 75-79 0.2877604390230246 2113 80-84 0.3515011335722349 2113 85-89 0.06304952496535066 2113 90-94 0.2578874760558634 2113 95-99 0.34704726121535145 2113 100-104 0.5222847804360171 2113 105-109 0.34870752531189453 2113 110-114 0.38498105921181747 2113 115-119 0.4190485513956048 2113 120-124 0.4531624445239828 2113 125-129 0.6523536946378172 2113 130-134 0.6664400031097912 2113 135-139 0.5958985944590083 2113 140-144 0.39958346147886914 2113 145-149 0.6611113987506201 2113 150-154 0.536125880714593 2113 155-159 0.5711557530890801 2113 160-164 0.2215545392521392 2113 165-169 0.3633088109188094 2113 170-174 -11.310863100869888 2113 175-179 -0.7395592613896333 2113 180-184 -0.06964061004480016 2113 185-189 0.4968649835033503 2113 190-194 1.0518706336430341 2113 195-199 1.2969562345668386 2113 200-204 -3.224208088320843 2113 205-209 -3.198822824325145 2113 210-214 0.6963114180650081 2113 215-219 0.833297986894447 2113 220-224 0.5441092053805505 2113 225-229 0.3877168998798375 2113 230-234 0.7795474627529941 2113 235-239 0.5440915985531376 2113 240-244 1.0969887986498321 2113 245-249 0.5468073265031848 2113 250-251 0.18124664223058673 1112 1 0.17519400761370463 1112 2 0.09907306090045864 1112 3 0.12667379826346092 1112 4 0.18141830703871165 1112 5 0.36880344495324024 1112 6 0.25885301603081246 1112 7 0.3266634107250752 1112 8 0.4130109462191953 1112 9 0.4481262248187079 1112 10-14 0.27908122791368584 1112 15-19 0.2744198785499421 1112 20-24 0.554038159891121 1112 25-29 0.3883185307442787 1112 30-34 0.2976712860050199 1112 35-39 0.16238559142647802 1112 40-44 0.2568859522739686 1112 45-49 0.23562535727347012 1112 50-54 0.23929044895731266 1112 55-59 0.41211848977688703 1112 60-64 0.22603299651350994 1112 65-69 0.4400843899408713 1112 70-74 0.40709646589441206 1112 75-79 0.2863015380317009 1112 80-84 0.5497630462975565 1112 85-89 0.38142528000164333 1112 90-94 0.2346034627960023 1112 95-99 0.3853095127964181 1112 100-104 0.39432562182182807 1112 105-109 0.3818161619440801 1112 110-114 0.27665945018854643 1112 115-119 -0.17612111961727095 1112 120-124 0.27677858201573 1112 125-129 0.9067272588289939 1112 130-134 0.5194714496855042 1112 135-139 0.33045648168985764 1112 140-144 0.09671156155192051 1112 145-149 0.3489385842473425 1112 150-154 0.6352321145964055 1112 155-159 0.5156021749383584 1112 160-164 -0.36553024463256634 1112 165-169 0.472589737789761 1112 170-174 -7.244714072316878 1112 175-179 -0.27503852173067145 1112 180-184 0.15555161624777725 1112 185-189 0.5752471170762732 1112 190-194 1.3054632724185922 1112 195-199 1.3662407355070343 1112 200-204 -2.8314667905670134 1112 205-209 -3.2799514915326107 1112 210-214 0.698747147373151 1112 215-219 0.8852639521022176 1112 220-224 0.6951694597715594 1112 225-229 0.6704700646837587 1112 230-234 0.9178929794833728 1112 235-239 0.556129325942635 1112 240-244 0.7315734171328465 1112 245-249 0.5766092150241704 1112 250-251 0.28191614643170126 2114 1 0.18872373304397172 2114 2 0.12850631110447353 2114 3 0.11166577200270211 2114 4 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10-14 -0.06550798032694871 2110 15-19 -0.10533264829881261 2110 20-24 -0.3248521140042371 2110 25-29 -0.2926029116172941 2110 30-34 -0.17918464122791278 2110 35-39 -0.043996719390406724 2110 40-44 -0.1644358456591064 2110 45-49 -0.2789368202362539 2110 50-54 -0.1676245711120643 2110 55-59 -0.19879978945783705 2110 60-64 -0.25728284724380046 2110 65-69 -0.43968800065295 2110 70-74 -0.2673055506637354 2110 75-79 -0.2324429084674975 2110 80-84 -0.4807814522789968 2110 85-89 -0.012989731018514306 2110 90-94 -0.43093721502541626 2110 95-99 -0.5626031505449447 2110 100-104 -0.27795367035424334 2110 105-109 -0.2501191385370376 2110 110-114 -0.1798025148552611 2110 115-119 -0.13012976852933633 2110 120-124 -0.36911642973705483 2110 125-129 -0.6505394622492418 2110 130-134 -0.5042415385221304 2110 135-139 -0.7806946558235346 2110 140-144 -0.3904278990723924 2110 145-149 -0.12713191530936285 2110 150-154 -0.4431140324350906 2110 155-159 -0.283675852522272 2110 160-164 -0.17821860343224216 2110 165-169 0.010848480626698631 2110 170-174 0.20983664589275364 2110 175-179 -0.6575320386619943 2110 180-184 -1.4860534956813218 2110 185-189 -0.1829465350220083 2110 190-194 0.048265092089902595 2110 195-199 -0.11828784124211467 2110 200-204 1.0581329246370217 2110 205-209 1.5305454813293444 2110 210-214 -0.556648742503981 2110 215-219 -0.5690079782022543 2110 220-224 -0.2557764800039486 2110 225-229 -0.10790317585918885 2110 230-234 -0.5528556193423739 2110 235-239 -0.05826125471539356 2110 240-244 0.10088467317824268 2110 245-249 0.04864293758046756 2110 250-251 0.04807789549401065 2109 1 -0.10224399785710148 2109 2 0.021216482905749956 2109 3 -0.01563094188106362 2109 4 -0.0334341735515693 2109 5 -0.12955875800922456 2109 6 0.030798451055517262 2109 7 -0.12843739790001507 2109 8 -0.1375729975530433 2109 9 -0.09903547297906812 2109 10-14 0.04076024572943737 2109 15-19 -0.21925646213998817 2109 20-24 -0.24708422125173257 2109 25-29 -0.2722129711208794 2109 30-34 -0.30130971276009433 2109 35-39 -0.18578561853249198 2109 40-44 -0.2885298058433179 2109 45-49 -0.2211251597485102 2109 50-54 -0.21231008441043997 2109 55-59 -0.5266786898894011 2109 60-64 -0.5288147617902652 2109 65-69 -0.25160949866872784 2109 70-74 -0.35472058118446625 2109 75-79 -0.24153645861043316 2109 80-84 -0.6118021216792968 2109 85-89 -0.24627593706230044 2109 90-94 -0.3794818478452555 2109 95-99 -0.6770583864357604 2109 100-104 -0.37660385418741527 2109 105-109 -0.5269301699015543 2109 110-114 -0.3684676532923703 2109 115-119 -0.9966669331349607 2109 120-124 -0.6886072783930643 2109 125-129 -0.5179930914652289 2109 130-134 -0.2697263722695169 2109 135-139 -0.8592140896615383 2109 140-144 -0.18951643350848002 2109 145-149 -0.2482161731454724 2109 150-154 -0.20056603175634535 2109 155-159 -0.3476185152782989 2109 160-164 -0.051843939230906244 2109 165-169 -0.5119611217643651 2109 170-174 0.49689853728935063 2109 175-179 -0.6294743970188712 2109 180-184 0.7911562095452496 2109 185-189 0.16597402386853588 2109 190-194 0.18553108356049108 2109 195-199 -0.18253146118986763 2109 200-204 1.8688055733514801 2109 205-209 1.7361951513266618 2109 210-214 -0.8019233223956448 2109 215-219 -1.3258376228374011 2109 220-224 -0.4628405000793556 2109 225-229 -0.6185735232156482 2109 230-234 -0.9969571889309954 2109 235-239 0.002843550905652137 2109 240-244 -0.35083897795203356 2109 245-249 -0.23175513961882643 2109 250-251 0.08468070581253073 2108 1 0.0034029725987281267 2108 2 0.03594718765851468 2108 3 0.10656205866680324 2108 4 -0.04563973945150934 2108 5 -0.27454984727392784 2108 6 0.07678034254114152 2108 7 -0.15666565416113087 2108 8 -0.04148106730743706 2108 9 -0.06009887740588482 2108 10-14 -0.03491706675097106 2108 15-19 -0.2410535392683073 2108 20-24 -0.2592335545354416 2108 25-29 -0.10939046444461553 2108 30-34 -0.17559622488462168 2108 35-39 -0.12855937729835176 2108 40-44 -0.2341377144688579 2108 45-49 -0.257428182972248 2108 50-54 -0.18330542360805424 2108 55-59 -0.23962325016799468 2108 60-64 -0.2849796973045855 2108 65-69 -0.4092146144227229 2108 70-74 -0.22614214281892941 2108 75-79 -0.20012425682296708 2108 80-84 -1.0329003840422288 2108 85-89 -0.40420293185081846 2108 90-94 -0.49003007468316184 2108 95-99 -0.23342263207961622 2108 100-104 -0.3957076061967939 2108 105-109 -0.47567177365773716 2108 110-114 -0.3075819262159243 2108 115-119 -0.4750652975122662 2108 120-124 -0.5516902467801899 2108 125-129 -0.72695281827815 2108 130-134 -0.37125508319174116 2108 135-139 -0.40564918505023684 2108 140-144 0.05737092857734183 2108 145-149 -0.18942467274433739 2108 150-154 -0.27145065726869433 2108 155-159 -0.2895622713060817 2108 160-164 0.05378214687759453 2108 165-169 -0.5665403223473007 2108 170-174 0.2468348054469196 2108 175-179 -0.384571020163083 2108 180-184 -0.7429638572631454 2108 185-189 0.031243209414441253 2108 190-194 0.11206029118027061 2108 195-199 0.36600225948722453 2108 200-204 1.9348489970314304 2108 205-209 1.6285243341835276 2108 210-214 -1.186015850544834 2108 215-219 -0.943769191425119 2108 220-224 -0.16999664367440204 2108 225-229 -0.44206663463597806 2108 230-234 -0.44093893874092416 2108 235-239 -0.1536020449320823 2108 240-244 -0.22532393208500423 2108 245-249 -0.16823966378797905 2108 250-251 0.218657104950708 >>END_MODULE >>Per sequence quality scores pass #Quality Count 12 7.0 13 166.0 14 844.0 15 1135.0 16 776.0 17 1010.0 18 3237.0 19 11622.0 20 35047.0 21 89173.0 22 159201.0 23 229931.0 24 300634.0 25 380587.0 26 493787.0 27 666298.0 28 853927.0 29 1073345.0 30 1295850.0 31 1484067.0 32 1681899.0 33 1953155.0 34 2419319.0 35 3201440.0 36 3307145.0 37 672662.0 38 567.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 8.522598824590313 1.9139992846325296 86.93455687060646 2.6288450201707145 2 0.7200778507238653 97.05853732799176 1.1926663169073957 1.0287185043769866 3 0.7076595754524906 1.2061625161916245 1.1139827859964972 96.97219512235938 4 72.01535515061379 25.38608506415198 1.0469250839365647 1.5516347012976581 5 50.90427734522181 15.617908127502758 32.65701722872036 0.8207972985550748 6 13.294455222864235 84.6110842778581 1.0413878030486152 1.0530726962290526 7 93.82375627380077 1.837830909751624 1.2924850337141653 3.0459277827334392 8 93.73406708949835 2.540317434347906 1.4907196895027575 2.234895786650979 9 79.43910150160721 3.2301149721627356 14.259271044780556 3.071512481449494 10-14 32.185149347356386 32.70213647000361 11.892553518804187 23.220160663835813 15-19 38.14375086350819 2.3674450016343593 39.50226095792203 19.986543176935417 20-24 34.696051761222016 9.131853289521382 28.788826367655467 27.38326858160114 25-29 20.388044970084607 33.84027197785198 38.584150634359766 7.187532417703651 30-34 39.236565380069024 20.293094137497075 25.04391482159708 15.426425660836825 35-39 37.597867257897995 20.452432182015635 22.328991609547604 19.62070895053877 40-44 47.7896301839593 41.80116869604319 1.5164077507953873 8.892793369202117 45-49 34.17066076889649 4.141086766927382 26.750700441422186 34.93755202275394 50-54 57.87749280387281 20.46399362183994 4.467745978691264 17.190767595595986 55-59 34.16707753290855 9.812009559955486 28.750763344933077 27.27014956220288 60-64 30.705220710848064 37.30833415900344 20.03952486487681 11.946920265271684 65-69 31.736993825464214 17.60599770702429 26.928798098482975 23.728210369028517 70-74 43.21767897759252 20.29732589693737 30.827949496651325 5.6570456288187865 75-79 7.442600669366202 41.75762647235683 17.90594310697372 32.893829751303244 80-84 51.14980185640172 10.007349079194487 8.15906181431543 30.68378725008836 85-89 1.541091718487002 38.35874403837882 27.007311327243897 33.09285291589028 90-94 31.62450974760778 6.0998164526741405 17.342235115309077 44.933438684409 95-99 31.40161967188682 35.78899878627725 19.296327266786832 13.513054275049097 100-104 28.81286681779129 10.741945047846734 25.461163952795236 34.98402418156674 105-109 25.805390614313815 44.394295547371534 19.55728922487961 10.243024613435038 110-114 26.909514579316035 11.75321978117552 25.22490441545731 36.112361224051135 115-119 33.00066531045122 12.871991699886662 30.172981209520323 23.954361780141795 120-124 42.32472574093863 30.075617600008584 22.74484441003619 4.854812249016591 125-129 45.88218605549261 14.710945816303733 16.636292343033222 22.770575785170433 130-134 61.38204427649174 35.04438758190192 2.0361689281167914 1.5373992134895447 135-139 37.68698413337182 25.82766894923395 22.326260648880336 14.159086268513887 140-144 38.58845801296472 39.79239281952977 18.66322754764264 2.9559216198628615 145-149 19.0680359458425 10.032418048192831 29.07465961669573 41.82488638926894 150-154 41.67238089444166 19.724799600882637 37.44852826703141 1.154291237644296 155-159 57.178538326178916 7.712021623844782 19.466789874857945 15.642650175118353 160-164 22.111425743512854 56.23482914239922 20.210609617218356 1.4431354968695658 165-169 35.10802349047447 18.640955373404445 23.18769497073633 23.063326165384748 170-174 23.863998949729613 39.20600236905869 35.00764010618298 1.9223585750287109 175-179 42.26110361404295 25.850727409210624 12.05094239352584 19.837226583220584 180-184 35.5340909219553 44.50110944959871 2.253045270692068 17.71175435775392 185-189 23.97483150792562 31.09353717614721 4.450993366042175 40.480637949885 190-194 57.16712076964288 3.0166259985784794 19.47163334155443 20.344619890224216 195-199 39.01951834909686 40.06010583048114 1.3643564786260218 19.55601934179597 200-204 38.574586662443856 13.651231069446423 18.889559277701423 28.884622990408293 205-209 39.71349961024925 6.15423438822718 26.269586039279453 27.862679962244112 210-214 29.263699927057523 24.630936041461744 13.265381921672548 32.83998210980819 215-219 21.67627717137579 19.192219495254946 34.96227339785422 24.16922993551504 220-224 19.607153300630397 22.448020559899327 19.058482102843698 38.88634403662658 225-229 50.080419529994614 24.153878131879917 8.485936610881883 17.279765727243586 230-234 35.046790515705915 39.8110305686945 1.9107527153225816 23.231426200277003 235-239 34.92417099891219 25.799633811001332 15.407861590225366 23.86833359986112 240-244 76.78743599333971 18.135968153694837 3.1254815280985504 1.9511143248669045 245-249 24.589147785892397 50.09007064143025 2.1109640573374855 23.209817515339868 250-251 18.203638648173033 32.29928673423528 1.2395461674116401 48.25752845018005 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.3333333333333333 17 0.3333333333333333 18 1.3333333333333333 19 6.333333333333331 20 6.999999999999997 21 9.666666666666666 22 18.333333333333336 23 31.66666666666662 24 38.666666666666664 25 45.666666666666714 26 55.666666666666785 27 69.33333333333343 28 101.99999999999963 29 116.33333333333276 30 171.33333333333383 31 442.99999999999227 32 759.3333333333497 33 620.0000000000006 34 731.6666666666799 35 966.3333333333733 36 1547.666666666594 37 2490.333333333348 38 3539.333333333825 39 8061.333333330472 40 18221.333333338567 41 24991.333333313938 42 31917.999999955406 43 47489.666666726094 44 59219.333333478105 45 73515.00000007462 46 100209.66666635283 47 123229.99999935117 48 107005.3333329206 49 103752.33333296794 50 153793.666666565 51 304559.33333391615 52 602596.9999969093 53 794985.0000193062 54 1051601.0000481238 55 1336963.9999816825 56 1336937.333315022 57 1149189.3333587355 58 957624.0000382399 59 677446.3333389562 60 394475.33332868235 61 244593.66666920763 62 167761.33333363818 63 93680.33333311451 64 54457.00000011012 65 35380.66666663799 66 25408.33333331242 67 19346.666666667807 68 14503.333333341832 69 11849.333333337005 70 10253.666666667435 71 8695.333333331268 72 6710.6666666650335 73 4217.666666667301 74 1940.3333333331714 75 715.0000000000114 76 270.0000000000021 77 153.66666666666666 78 90.66666666666646 79 65.00000000000016 80 42.00000000000002 81 23.999999999999982 82 22.999999999999986 83 19.0 84 14.666666666666675 85 15.333333333333343 86 13.666666666666673 87 12.666666666666671 88 13.000000000000005 89 18.666666666666668 90 15.666666666666677 91 10.666666666666668 92 13.000000000000005 93 7.9999999999999964 94 6.333333333333331 95 5.333333333333332 96 1.9999999999999998 97 3.0 98 11.333333333333336 99 14.000000000000007 100 48.5 >>END_MODULE >>Per base N content warn #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 9.844054911910228E-7 30-34 9.844054911910228E-7 35-39 1.9688109823820457E-6 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 9.844054911910228E-7 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 9.844054911910228E-7 140-144 0.0 145-149 9.844054911910228E-7 150-154 0.0 155-159 0.0 160-164 0.0 165-169 0.0 170-174 0.7374860774300874 175-179 0.0 180-184 0.0 185-189 0.0 190-194 9.844054911910228E-7 195-199 0.0 200-204 7.194870105480525 205-209 0.0 210-214 9.844054911910228E-7 215-219 0.0 220-224 0.0 225-229 0.0 230-234 0.0 235-239 0.0 240-244 0.0 245-249 0.0 250-251 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 251 2.0316831E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 4.434372991615954 #Duplication Level Percentage of deduplicated Percentage of total 1 71.78234904469812 3.183097098785587 2 10.518248845489026 0.9328367719906446 3 4.013463749611026 0.5339158576231449 4 2.4100221875586785 0.42747749190821616 5 1.6125723366292124 0.35753736082878046 6 1.2644307225568985 0.33641744675254537 7 0.8973195288091065 0.2785334638380459 8 0.49219670734242904 0.17460670284812554 9 0.6145415997050739 0.24525960047609785 >10 4.468489969930078 4.26764295150866 >50 0.9737597216671464 3.042980552447837 >100 0.7124493990458711 6.4811322708417025 >500 0.09865341384479302 3.0594615458316805 >1k 0.10025571702042516 9.52845103867766 >5k 0.016632456444835825 5.128549570143057 >10k+ 0.024614599647373635 62.02210027549832 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source TACGGGGGGGGCAAGCGTTGTTCGGAATTACTGGGCGTAAAGGGCTCGTA 572138 2.816078944595247 No Hit TACAGAGGGGGCAAGCGTTGTTCGGAATTACTGGGCGTAAAGGGCGCGTA 498891 2.455555199528903 No Hit TACGTAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGTGCGCA 448916 2.209576877417546 No Hit TACGTAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGTGTGCGCA 365410 1.7985580526805582 No Hit TACGAAGGGGGCTAGCGTTGCTCGGAATCACTGGGCGTAAAGCGCACGTA 338884 1.667996352383893 No Hit TACGGGGGGAGCAAGCGTTGTTCGGATTTACTGGGCGTAAAGGGCGCGTA 338581 1.6665049780647385 No Hit TACGGAGGGTGCGAGCGTTGTCCGGAATCACTGGGCGTAAAGGGCGCGTA 302070 1.4867968336203614 No Hit TACGTAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGTGCGCA 263900 1.2989230456265548 No Hit TACAGAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGTA 259514 1.2773350332047355 No Hit TACAGAGGTGGCAAGCGTTGTTCGGAATTACTGGGCGTAAAGGGCGCGTA 254105 1.2507117866954742 No Hit TACGTAGGGGGCAAGCGTTGTCCGGATTTATTGGGCGTAAAGAGCGTGTA 200616 0.9874374601038911 No Hit TACGGAGGGGGCTAGCGTTGTTCGGAATTACTGGGCGTAAAGCGCACGTA 197770 0.9734293699642429 No Hit TACGTAGGTGGCAAGCGTTGTCCGGATTTACTGGGCGTAAAGAGCGCGCA 185535 0.9132083640406321 No Hit TACGGAGGGTGCAAGCGTTATCCGGATTCACTGGGTTTAAAGGGTGCGTA 180549 0.8886671351452399 No Hit TACAGAGGTCTCAAGCGTTGTTCGGATTCATTGGGCGTAAAGGGTGCGTA 167853 0.8261770745644338 No Hit TACGGGGGGGGCAAGCGTTGTTCGGAATTACTGGGCGTAAAGGGCGCGTA 167344 0.8236717625893526 No Hit TACAGAGGTCCCAAGCGTTGTTCGGATTCACTGGGCGTAAAGGGTGCGTA 156401 0.7698100161388358 No Hit TACGTAGGGTGCAGGCGTTAATCGGAATTACTGGGCGTAAAGCGTGCGCA 152633 0.751263816684797 No Hit TACGGAGGGGGCTAGCGTTGTTCGGAATTACTGGGCGTAAAGCGCGCGTA 147162 0.7243354044732665 No Hit GACAGAGGGGGCAAGCGTTGCTCGGAATCACTGGGCGTAAAGGGCGCGTA 146866 0.7228784843463039 No Hit TACAGAGGGTGCAAGCGTTGTTCGGAATCATTGGGCGTAAAGGGCGTGTA 136945 0.6740470499557731 No Hit TACAGAGGTCCCGAGCGTTGTTCGGATTCACTGGGCGTAAAGGGTGCGTA 135572 0.6672891062587467 No Hit TACAGAGGGTGCGAGCGTTGTCCGGAATCACTGGGCGTAAAGGGCGCGTA 134736 0.6631742913055683 No Hit TACGAAGGGTGCAAGCGTTACTCGGAATTACTGGGCGTAAAGCGTGCGTA 133951 0.6593104997526436 No Hit TACGTAGGGACCAAGCGTTGTTCGGATTTACTGGGCGTAAAGGGCGCGTA 123638 0.6085496305993784 No Hit TACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCA 118776 0.5846187331085246 No Hit TACAGAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGTGCGTA 117541 0.57854002920042 No Hit TACGAAGGGGGCTAGCGTTGTTCGGAATTACTGGGCGTAAAGCGCACGTA 115466 0.5683268222293132 No Hit TACGTAGGTGGCAAGCGTTGTCCGGATTTATTGGGTTTAAAGGGTGCGTA 112451 0.5534869094496085 No Hit TACCAGCACCCCGAGTGGTCGGGACGATTATTGGGCCTAAAGCATCCGTA 109997 0.5414082540726948 No Hit TACGTAGGGGGCTAGCGTTGTCCGGAATCATTGGGCGTAAAGCGCGCGTA 107545 0.5293394427506928 No Hit TACGTAGGGGGCAAGCGTTGTCCGGAATCATTGGGCGTAAAGAGCGTGTA 106706 0.5252098617151464 No Hit TACAGAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGCGCGTA 106394 0.5236741891488884 No Hit TACGAAGGGAGCTAGCGTTGTTCGGAATCACTGGGCGTAAAGCGCACGTA 99355 0.48902803788642035 No Hit TACGTAGGGGGCTAGCGTTGTCCGGAATCATTGGGCGTAAAGCGCGTGTA 98868 0.4866310105153702 No Hit TACGGAGGGAGCTAGCGTTGTTCGGAATTACTGGGCGTAAAGCGCACGTA 98573 0.4851790124158635 No Hit GACAGAGGGTGCAAACGTTGTTCGGAATTACTGGGCGTAAAGCGCGTGTA 82880 0.4079376355495599 No Hit TACGAAGGGGGCTAGCGTTGTTCGGAATTACTGGGCGTAAAGGGCGCGTA 81887 0.40305006228579643 No Hit TACGTAGGGGGCAAGCGTTGTCCGGATTCATTGGGCGTAAAGAGCGTGTA 78926 0.38847593898871335 No Hit TACGAAGGGGGCAAGCGTTGTTCGGAATTACTGGGCGTAAAGGGCGCGTA 78909 0.38839226452196207 No Hit TACGGGGGGGGCAAGCGTTGTTCGGAATTACTGGGCGTAAAGGGTTCGTA 77371 0.38082218629470316 No Hit TACGGAGGGGACTAGCGTTGTTCGGAATTACTGGGCGTAAAGCGCACGTA 77019 0.37908963263020695 No Hit TACGTAGGGAGCAAGCGTTGTTCGGATTTACTGGGCGTAAAGGGCGCGTA 75339 0.37082062650420233 No Hit TACAGAGGGTGCGAGCGTTAATCGGATTTACTGGGCGTAAAGCGTGCGTA 73602 0.3622710648132083 No Hit TACAGAGGGGGCAAGCGTTATTCGGAATTATTGGGCGTAAAGGGCGCGTA 72655 0.35760990481241883 No Hit TACGTAGGGTGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGAGCTCGTA 72006 0.354415508993504 No Hit TACGAAGGGGGCTAGCGTTGTTCGGATTTACTGGGCGTAAAGCGCACGTA 70459 0.3468011325191414 No Hit TACGGAGGGAGCTAGCGTTGTTCGGAATTACTGGGCGTAAAGCGCGCGTA 70347 0.34624986544407443 No Hit TACGTAGGTGGCAAGCGTTGTTCGGAATTATTGGGCGTAAAGGGCGCGTA 70261 0.3458265710828623 No Hit TACGTAGGGGGCAAGCGTTGTCCGGAATCATTGGGCGTAAAGCGCGCGTA 69491 0.34203660994177687 No Hit TACAGAGGGGGCTAGCGTTGTTCGGAATTACTGGGCGTAAAGGGCGCGTA 68942 0.3393344168684575 No Hit TACGAAGGTGGCAAGCGTTGTTCGGATTTACTGGGCGTACAGGGAGCGTA 65429 0.32204333441568717 No Hit TACGTAGGGGGCAAGCGTTGTCCGGAATCATTGGGCGTAAAGCGCGTGTA 64861 0.3192476228207047 No Hit TACGTAGGGGGCTAGCGTTGTCCGGATTTATTGGGCGTAAAGAGCGTGTA 62980 0.30998928917605306 No Hit TACGAAGGGGGCTAGCGTTGTTCGGAATCACTGGGCGTAAAGCGCACGTA 62133 0.3058203319208591 No Hit TACGAAGGGGGCTAGCGTTGCTCGGAATCACTGGGCGTAAAGGGTGCGTA 59304 0.2918959162479621 No Hit TACGTAGGTGGCGAGCGTTGTCCGGATTTACTGGGCGTAAAGAGCGCGCA 58903 0.2899221832381241 No Hit TACGTAGGTGACAAGCGTTGTCCGGATTTACTGGGCGTAAAGAGCGCGCA 58077 0.2858565885595052 No Hit GACAGAGGGGGCAAGCGTTGCTCGGAATTACTGGGCGTAAAGGGCGCGTA 56801 0.27957608152570645 No Hit TACGAAGGGGGCTAGCGTTGTTCGGAATCACTGGGCGTAAAGGGCGCGTA 54615 0.2688165295069886 No Hit TACGTAGGGGGCAAGCGTTGTCCGGATTTACTGGGCGTAAAGAGCGTGTA 54549 0.2684916756948955 No Hit GACGAACCGTGCGAACGTTGTTCGGAATCACTGGGCTTAAAGGGCGCGTA 52133 0.256600057361308 No Hit TACGAAGGGGGCTAGCGTTGCTCGGAATTACTGGGCGTAAAGCGCACGTA 51029 0.2511661390499335 No Hit CACGGGGGGAGCAAGCGTTGTTCGGAATCACTGGGCGTAAAGGGCGTGTA 50396 0.24805049567031395 No Hit TACGTAGGGCGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGAGCTCGTA 50034 0.2462687217312582 No Hit TACGGAGGGTGCAAACGTTGCTCGGAATCATTGGGCGTAAAGCGCACGTA 49290 0.2426067333040276 No Hit TACGAAGGGGGCTAGCGTTGCTCGGAATTACTGGGCGTAAAGGGCGCGTA 48638 0.23939757140274484 No Hit TACGTAGGGGGCTAGCGTTGTCCGGAATCATTGGGCGTAAAGAGCGTGTA 47937 0.23594723015612032 No Hit TACGGGGGGGGCTAGCGTTGTTCGGAATTACTGGGCGTAAAGGGCTCGTA 47471 0.23365356536164525 No Hit TACGTAGGGTGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGAGCTCGTA 47031 0.231487873281025 No Hit TACGAAGGGGGCTAGCGTTGTTCGGATTTACTGGGCGTAAAGCGCGTGTA 47024 0.2314534190888333 No Hit TACGTAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGGGTGCGCA 46124 0.2270235943784737 No Hit TACGTAGGGTGCGAGCGTTGTCCGGAATTACTGGGCGTAAAGAGCTCGTA 45834 0.22559620641624673 No Hit TACAGAGGGTGCAAGCGTTGTTCGGAATTATTGGGCGTAAAGCGCGTGTA 44449 0.21877919838974888 No Hit CACGTAGGGGACGAGCGTTGTCCGGATTCATTGGGCGTAAAGCGCGCGTA 44345 0.21826730753432955 No Hit GACGGAGGGTGCAAACGTTGTTCGGAATCACTGGGCGTAAAGGGCGCGTA 44281 0.21795229777714842 No Hit TACGGGGGGGGTAAGCGTTGTTCGGAATTACTGGGCGTAAAGGGCTCGTA 43857 0.21586535813582344 No Hit TACAGAGGGCGCGAGCGTTGTCCGGAATCACTGGGCGTAAAGGGCGCGTA 43831 0.2157373854219686 No Hit GACAGAGGGTGCAAACGTTGCTCGGAATTACTGGGCGTAAAGCGCGTGTA 43667 0.21493017291919198 No Hit TACGAAGGGGGCTAGCGTTGTTCGGAATTACTGGGCGTAAAGCGCGCGCA 43625 0.21472344776604185 No Hit TACGAAGGGTGCAAACGTTGCTCGGAATTATTGGGCGTAAAGCGCACGTA 43490 0.21405897405948793 No Hit TACAGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCGCGTA 43472 0.21397037756528073 No Hit TACAGAGACTGCAAGCGTTATTCGGATTCACTGGGCGTAAAGGGTGCGCA 42987 0.21158319424914251 No Hit TACGTAGGGGGCAAGCGTTGTCCGGATTTATTGGGCGTAAAGCGCGTGTA 40886 0.2012420145641808 No Hit GACGTAGGGCGCGAGCGTTGTCCGGATTTATTGGGCGTAAAGAGCTCGTA 40093 0.1973388467916084 No Hit TACGTAGGGAGCAAGCGTTGTCCGGAATCATTGGGCGTAAAGCGCGCGTA 39790 0.195847472472454 No Hit TACGGAGGGTGCGAGCGTTATCCGGAATTACTGGGTTTAAAGGGTGCGTA 38663 0.19030034752959257 No Hit TACGTAGGGCGCAAGCGTTATCCGGAATTATTGGGCGTAAAGAGCTCGTA 38309 0.18855794981018448 No Hit TACGTAGGGTGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGGGCTCGTA 37539 0.18476798866909902 No Hit TACGGGGGGGGCAAGCGTTGTTCGGAATTACTGGGCGTAAAGGGTGCGTA 36486 0.1795850937579783 No Hit TACGTAGGGGGCAAGCGTTGTCCGGATTTACTGGGCGTAAAGCGCGTGTA 36198 0.17816754985066321 No Hit TACGAAGGGGGCTAGCGTTGCTCGGAATCACTGGGCGTAAAGGGCGCGTA 36139 0.1778771502307619 No Hit TACGTAGGGGGCGAGCGTTGTCCGGATTTATTGGGCGTAAAGAGCTCGTA 36032 0.1773504932929747 No Hit TACAGGGGGGGCAAGCGTTGTTCGGAATTACTGGGCGTAAAGGGCTCGTA 35525 0.17485502537280545 No Hit TACGTAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGTGCGTA 35267 0.173585142289169 No Hit TACGTAGGGTGCGGGCGTTAATCGGAATTACTGGGCGTAAAGCGTGCGCA 33853 0.16662539546644847 No Hit TACAGAGGGGGCGAGCGTTGTTCGGAATTACTGGGCGTAAAGGGCGCGTA 33522 0.16499620437852733 No Hit TACGAAGGGGGCTAGCGTTGTTCGGAATTACTGGGCGTAAAGCGCGCGTA 32434 0.15964103850644817 No Hit TACGTAGGTGGCGAGCGTTGTCCGGATTTACTGGGCGTAAAGCGCGCGCA 32417 0.15955736403969695 No Hit TACGTAGGGGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGAGCGTGTA 32194 0.15845975191701894 No Hit GACGTAGGGAGCGAGCGTTGTCCGGATTCATTGGGCGTAAAGCGCGCGTA 32165 0.15831701312079624 No Hit TACGTAGGGGGCTAGCGTTGTCCGGAATCATTGGGCGTAAAGAGCGCGTA 31850 0.1567665744721704 No Hit TACGTAGGGGGCAAGCGTTGTCCGGATTTATTGGGCGTAAAGAGCTCGTA 30789 0.151544303341402 No Hit TACGAAGGGGACTAGCGTTGTTCGGAATTACTGGGCGTAAAGCGCACGTA 29978 0.1475525390746224 No Hit TACGTAGGGTCCGAGCGTTGTCCGGAATTACTGGGCGTAAAGAGCTCGTA 29690 0.14613499516730735 No Hit TACGGAGGGGGCGAGCGTTATTCGGAATTATTGGGCGTAAAGGGCGCGTA 29178 0.14361491710985833 No Hit TACGGAGGTGGCAAGCGTTGCTCGGAATCACTGGGCGTAAAGGGCGCGTA 29121 0.1433343615448689 No Hit TACAGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGTGCGTA 29036 0.14291598921111268 No Hit TACGGAGGGTGCGAGCGTTGTTCGGAATCACTGGGCGTAAAGGGCGTGTA 29002 0.14274864027761022 No Hit TACAGGGGGGGCAAGCGTTGTTCGGAATTACTGGGCGTAAAGGGTTCGTA 28859 0.14204479035140866 No Hit TACGGAGGGGGCTAGCGTTGTTCGGAATTACTGGGCGTAAAGCGTACGTA 27924 0.1374426946800906 No Hit TACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCGCGTA 27704 0.13635984863978048 No Hit TACAGAGGGGGCCAGCGTTATTCGGAATTATTGGGCGTAAAGGGCGCGTA 26765 0.13173806485863865 No Hit TACGTAGGTGGCAAGCGTTGTCCGGATTTACTGGGCGTAAAGGGCGTGCA 26421 0.13004488741379008 No Hit TACAGAGGGTGCTAGCGTTGTTCGGAATCATTGGGCGTAAAGGGCGTGTA 26176 0.12883899068708107 No Hit TACGGAGGGTGCAAGCGTTATCCGGATTCACTGGGCTTAAAGGGTGCGTA 25479 0.12540833755028036 No Hit TACGAAGGGTGCAAACGTTGCTCGGAATCATTGGGCGTAAAGCGCACGTA 25362 0.12483246033793362 No Hit TACGTAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGCGCGCA 25097 0.1235281230621055 No Hit TACGGAGGGTGCAAGCGTTGTTCGGAATCACTGGGCGTAAAGGGCGTGTA 24789 0.12201213860567132 No Hit TACGTAGGGGGCAAGCGTTGTCCGGAATCATTGGGCGTAAAGAGCGCGTA 24689 0.12151993586007581 No Hit TACGTAGGGGGCAAGCGTTGTTCGGAATTACTGGGCGTAAAGGGCGCGTA 24586 0.12101296703211245 No Hit CACGTAGGGTGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGAGCTCGTA 24325 0.11972831786610816 No Hit CACGTAGGGGGCGAGCGTTGTCCGGATTTATTGGGCGTAAAGAGCTCGTA 22866 0.11254707980786964 No Hit TACGTAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGTGTGCGCA 22858 0.11250770358822201 No Hit TACGGAGGGGGCCAGCGTTGTTCGGAATCATTGGGCGTAAAGGGCGCGTA 22462 0.11055858071566378 No Hit TACAGAGGTCCCAAGCGTTGTTCGGATTTACTGGGCGTAAAGGGTGCGTA 22349 0.11000239161314085 No Hit TACGGAGGGAGCTAGCGTTGTTCGGAAATACTGGGCGTAAAGCGCACGTA 22308 0.10980058848744668 No Hit TACGGAGGGAGCTAGCGTTGTTCGGATATACTGGGCGTAAAGCGCACGTA 21219 0.10444050058791157 No Hit GACAGAGGTGCCAAGCGTTAGGCGGAATCACTGGGCTTAAAGCGTGTGTA 20893 0.1028359196372702 No Hit TACAGAGGGGGCAAGCGTTGTTCGGAATTACTGGGCGTAAAGGGCTCGTA 20814 0.10244707946824975 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 2.018031256941597E-4 0.0 0.0 2 2.1164718060606992E-4 0.0 0.0 3 2.1656920806202503E-4 0.0 0.0 4 2.1656920806202503E-4 0.0 0.0 5 2.1656920806202503E-4 0.0 0.0 6 2.1656920806202503E-4 0.0 0.0 7 2.3133529042989036E-4 0.0 0.0 8 2.461013727977557E-4 0.0 0.0 9 2.461013727977557E-4 0.0 0.0 10-14 2.57914238692048E-4 0.0 0.0 15-19 2.825243759718236E-4 0.0 0.0 20-24 3.03196891286835E-4 0.0 0.0 25-29 3.317446505313747E-4 0.0 0.0 30-34 4.7251463577169093E-4 0.0 0.0 35-39 5.847368617674677E-4 0.0 0.0 40-44 7.038499262015813E-4 0.0 0.0 45-49 8.908869695278756E-4 0.0 0.0 50-54 0.001073986390889406 0.0 0.0 55-59 0.0013978557974912524 0.0 0.0 60-64 0.0017916179939676616 0.0 0.0 65-69 0.0021814425684793066 0.0 0.0 70-74 0.0027494445368965266 0.0 0.0 75-79 0.0033292593712080398 0.0 0.0 80-84 0.004136471873984678 0.0 0.0 85-89 0.00517698847817359 0.0 0.0 90-94 0.006604376440400574 0.0 0.0 95-99 0.008368431080614887 0.0 1.9688109823820457E-6 100-104 0.010696550067281655 0.0 4.922027455955114E-6 105-109 0.013387914680197913 0.0 4.922027455955114E-6 110-114 0.016634483990145905 0.0 4.922027455955114E-6 115-119 0.02066168685460838 0.0 8.859649420719205E-6 120-124 0.029021258285802543 0.0 9.844054911910228E-6 125-129 0.03643186282348856 0.0 9.844054911910228E-6 130-134 0.04186381232388063 0.0 9.844054911910228E-6 135-139 0.04765802304503099 0.005106111282807836 9.844054911910228E-6 140-144 0.05381646379792203 0.016538012252009186 9.844054911910228E-6 145-149 0.0602899143079942 0.016538012252009186 9.844054911910228E-6 150-154 0.06679388138829327 0.016538012252009186 1.4766082367865342E-5 155-159 0.07381663016245003 0.016538012252009186 1.4766082367865342E-5 160-164 0.08031370640431078 0.016538012252009186 1.4766082367865342E-5 165-169 0.08690430116783468 0.016538012252009186 1.4766082367865342E-5 170-174 0.09369669905705275 0.016538012252009186 1.4766082367865342E-5 175-179 0.10075094880692761 0.016538012252009186 1.4766082367865342E-5 180-184 0.10804834671312666 0.016538012252009186 1.4766082367865342E-5 185-189 0.11559676801957944 0.016538012252009186 1.4766082367865342E-5 190-194 0.12253387351600258 0.016538012252009186 1.4766082367865342E-5 195-199 0.12735844482832975 0.016538012252009186 1.4766082367865342E-5 200-204 0.13236020912907134 0.016538012252009186 1.4766082367865342E-5 205-209 0.1392333282685671 0.016538012252009186 1.4766082367865342E-5 210-214 0.147375346086208 0.016538012252009186 1.4766082367865342E-5 215-219 0.1550418960516037 0.016538012252009186 1.771929884143841E-5 220-224 0.16329219847327567 0.016538012252009186 1.9688109823820457E-5 225-229 0.17119205254008363 0.016538012252009186 1.9688109823820457E-5 230-234 0.17943054209586132 0.016538012252009186 1.9688109823820457E-5 235-239 0.18792891470131343 0.016538012252009186 1.9688109823820457E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCAAACA 625980 0.0 240.32376 245 TACGAAG 225455 0.0 239.37657 1 TACAGAG 400775 0.0 239.34074 1 CGTAGGG 499680 0.0 239.26181 3 GTGCGAG 299320 0.0 238.5942 9 GGTCCCA 29215 0.0 238.53415 7 GCAACCA 82410 0.0 237.903 245 AGGTCCC 52540 0.0 237.72867 6 GTAGGGT 236880 0.0 236.74277 4 GCGAACA 140940 0.0 236.73265 245 TACGGAG 299890 0.0 236.69896 1 ACGGAGG 313245 0.0 236.50533 2 TACGTAG 578275 0.0 236.09138 1 TCAAACA 48375 0.0 235.68927 245 ACAGAGG 472470 0.0 235.33844 2 AGGGGGC 558825 0.0 235.01103 6 TACGGGG 199615 0.0 234.36049 1 CGAAGGG 218765 0.0 232.05374 3 GAACCGT 17490 0.0 231.90596 4 GACGTAG 27015 0.0 231.46416 1 >>END_MODULE