FastQCFastQC Report
Thu 3 Sep 2015
000000000-AHF14 l01n03.3330000003439d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AHF14 l01n03.3330000003439d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6667600
Sequences flagged as poor quality0
Sequence length251
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTTTGATCCCCACGCTTTCGCACATCAGCGTCAGTTACAGACCAGAA148558722.280685704001442No Hit
CCTGTTCGCTACCCATGCTTTCGCTCCTCAGCGTCAGTAACTGCCCAGTA3246684.86933829263903No Hit
CCCAGTTTGGGTCTTAGCTATTGTGTGTTCAGATATGTTAAAGCCACTTT2771844.157177995080689No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTGTTATCCCAGGA2335343.502519647249385No Hit
CCTGTTTGCTCCCCACGCTTTCGTACCTCAGCGTCAGTTTGTGTCCAGAA1707212.5604565360849483No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAAGCCAGAG1693672.5401493790869276No Hit
CCCGTTCGCTCCCCTGGCTTTCGCGCCTCAGCGTCAGTTTTCGTCCAGAA1084981.6272421860939468No Hit
CCTGTTCGCTCCCCATGCTTTCGCTTCTCAGCGTCAGTTACAGCCCAGAG653360.9799028136060952No Hit
CCTGTTTGCTACCCACGCTTTCGAGCATGAACGTCAGTGTTATCCCAGGA622450.9335443037974683No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG614330.9213660087587737No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTGTTGGTCCAGGT506900.7602435659007739No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTAACGGCCCAGAG503810.7556092147099406No Hit
CCTGTTTGATACCCGCACCTTCGAGCTTAAGCGTCAGTTGCGCTCCCGTC450470.6756104145419641No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTGAGCGTCAGTCTTCGTCCAGGG327350.4909562661227428No Hit
CCTGTTTGCTACCCACGCTTTCGTTCCTCAGCGTCAGAATGAGCCCAGTA311320.4669146319515267No Hit
CCTGTTCGCTACCCATGCTTTCGCTCCTCAGCGTCAGTTACTGCCCAGAG264580.39681444597756316No Hit
CCCATTTGCTCCCCTAGCTTTCGTCTCTCAGTGTCAGTGTCGGCCCAGCA257580.386315915771792No Hit
CCCATTCGCTCCCCTAGCTTTCGTCTCTCAGTGTCAGTGTCGGCCCAGCA254550.38177155198272245No Hit
CCTGTTCGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG246390.369533265342852No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTGAGCGTCAGTCTTTGTCCAGGG214190.3212400263963045No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTACAGGTCCAGGG210490.3156908032875398No Hit
CCTGTTTGATACCCACGCTTTCGTGCTTCAGCGTCAGTTGTACCTTAGTA169740.2545743595896574No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGAAATAGGCCAGCA153400.23006779050932868No Hit
CCTGTTCGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAC133420.20010198572199892No Hit
CCTGTTTGCTACCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG129280.19389285500029996No Hit
CCTGTTTGATCCCCACGCTTTCGCACATCAGCGTCAGTTCCAGACCAGAA128000.19197312376267323No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGTGTCAGTATCAGTCCAGGT109620.16440698302237686No Hit
CCTGTTCGCTCCCCATGCTTTCGCTCCTCAGCGTCAGTTACTGCCCAGAG108470.16268222448857161No Hit
CCTGTTTGCTACCCACGCTTTCGTACCTCAGCGTCAGTTAATGGCCAGAA108270.1623822664826924No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTACAGGCCCAGGG104960.15741796148539203No Hit
CCTGTTCGCTCCCCATGCTTTCGCTCCTCAGCGTCAGTTACAGCCCAGAG101060.15156878037074809No Hit
CCTGTTTGCTCCCCATGCTTTCGTACCTCAGCGTCAGTATTAGGCCAGAT100760.15111884336192932No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGTGTCAGTATTAGCCCAGGT97440.146139540464335No Hit
CCTGTTTGCTCCCCACGCTTTCGCACATCAGCGTCAGTTACAGACCAGAA95130.1426750254964305No Hit
CCTATTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTCTTTGGCTAGCA94770.14213510108584798No Hit
CCTGTTTGCTACCCACGCTTTCGTACCTCAGCGTCAGATGATGACCAGAA92160.13822064910912474No Hit
CCTGTTTGCTCCCCACGCTTTCGCACATGAGCGTCAGTACATTCCCAAGG84480.12670226168336432No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGTGTCAGTTACAGGCCAAAA80200.12028316035754993No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAATCCAGAG79860.11977323174755534No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAACGTCAGTGTTATCCCAGGA78900.1183334333193353No Hit
CCCAGTTTGGGTCTTAGCTATTGTGTGTTCAGATGTGTTAAAGCCACTTT74070.11108944747735318No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTGTTATCCCAGGG73450.11015957765912773No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGGAAAGGCCCAGAG67550.101310816485692No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTTACTGCCCAGAG66760.10012598236246925No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTTTGA1738000.0244.61943
TGTTTGC1385400.0244.4963
CTGTTCG802500.0244.313062
TTTGATC1657150.0244.216425
GATCCCC1673100.0243.828498
TGATCCC1665350.0243.756857
TTGGGTC315650.0243.602897
TTTGCTC1226300.0243.501595
CCTGTTC813300.0243.237731
TTTGGGT316650.0242.678826
TTGCTCC1239250.0242.499086
GTTTGGG317800.0242.455955
TTGATCC1683300.0242.0096
CTGTTTG3180500.0239.846562
TGTTCGA32850.0237.168953
GTTTGAT1793300.0237.076074
ATCCCCA1729800.0236.508999
CCGTTCG143250.0236.448522
GTTTGCT1441050.0235.81074
CTCCCCA1576350.0235.433769
Produced by FastQC (version 0.11.2)