##FastQC	0.11.2
>>Basic Statistics	pass
#Measure	Value
Filename	000000000-AHF14 l01n02.33200000034390.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6667600
Sequences flagged as poor quality	0
Sequence length	12
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.400515627812105	32.0	16.0	33.0	16.0	34.0
2	27.821046853440517	32.0	16.0	33.0	16.0	34.0
3	27.745359499670045	32.0	16.0	33.0	16.0	34.0
4	27.987027266182736	32.0	16.0	33.0	16.0	34.0
5	27.934266152738616	32.0	16.0	34.0	16.0	34.0
6	29.949167016617672	34.0	18.0	37.0	16.0	37.0
7	29.884206761053452	34.0	18.0	37.0	16.0	37.0
8	29.641758953746475	33.0	18.0	37.0	16.0	37.0
9	29.85915036894835	34.0	18.0	37.0	16.0	37.0
10	29.87484852120703	34.0	18.0	37.0	16.0	37.0
11	30.03971728957946	34.0	18.0	37.0	16.0	37.0
12	29.397479302897594	33.0	18.0	37.0	16.0	37.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	-0.03624435144095628
1101	2	-0.07369605571362925
1101	3	-0.07003204607821445
1101	4	-0.1261908821915938
1101	5	-0.1289743512026149
1101	6	-0.1378168103870756
1101	7	-0.11915938136882431
1101	8	-0.12799732096307537
1101	9	-0.08793354458885361
1101	10	-0.10244299946617375
1101	11	-0.12839621309840865
1101	12	-0.11216916669521737
1102	1	0.012523327491138048
1102	2	-0.02506507160506999
1102	3	0.030550002263805
1102	4	0.001333349147408569
1102	5	-0.04708711077298133
1102	6	-0.002710247517480724
1102	7	0.027547360163264756
1102	8	0.01080792710715528
1102	9	-0.029662505628984093
1102	10	0.04423299099621403
1102	11	0.012693214272029962
1102	12	0.030034340317239838
1103	1	0.08735934349123653
1103	2	0.022343651728096603
1103	3	0.1459645291905005
1103	4	0.11455217030020748
1103	5	0.0342355114860915
1103	6	0.08716152276416267
1103	7	0.014907707327054709
1103	8	0.05931157600830872
1103	9	0.10217327525751685
1103	10	0.11212112178124656
1103	11	0.13731223743690535
1103	12	0.08489971458096335
2112	1	0.10553951704082465
2112	2	0.15212583943374725
2112	3	0.1409356421465624
2112	4	0.17126090282395978
2112	5	0.1111547099505259
2112	6	0.12609982213671955
2112	7	0.07442494833117053
2112	8	0.13579015063305988
2112	9	0.13716095393993655
2112	10	0.20209262430195452
2112	11	0.16474240676054563
2112	12	0.18242520774939663
1113	1	-0.09915665589442568
1113	2	-0.11907744468183168
1113	3	-0.0961289854433538
1113	4	-0.05547745171497098
1113	5	-0.06233750419624329
1113	6	4.3598715767956264E-4
1113	7	-0.11051382854706304
1113	8	-0.037812191705214104
1113	9	-0.02792811323642752
1113	10	-0.04410333341241568
1113	11	-0.04165791853486667
1113	12	-0.0580771534839819
2113	1	-0.042986719302000154
2113	2	-0.0031002431829527666
2113	3	0.04159970501518728
2113	4	-0.05311623664396592
2113	5	-0.038182984031749356
2113	6	-0.012923651198331498
2113	7	-0.06648474705631457
2113	8	-0.04612239674251839
2113	9	-0.15502194300613326
2113	10	-0.12763482554631267
2113	11	-0.0055261879668790925
2113	12	-0.023896457126841852
1112	1	0.24447643182607592
1112	2	0.07395690297910917
1112	3	0.19360617324949203
1112	4	0.1821543198554103
1112	5	0.1820234032487349
1112	6	0.11593889904799326
1112	7	0.105772669007969
1112	8	0.1459041603732949
1112	9	0.07224849814438272
1112	10	0.19826254200288673
1112	11	0.18854234761186106
1112	12	0.2658713418775349
2114	1	0.0667902347051772
2114	2	0.016257945839207366
2114	3	0.09379815768156874
2114	4	0.0955261223125845
2114	5	0.10222831958584777
2114	6	0.08457139560015747
2114	7	0.11943543718082594
2114	8	0.0261961492833116
2114	9	0.02599421018932091
2114	10	0.07324503321260778
2114	11	0.1499299261919269
2114	12	0.09384402950792392
1114	1	0.18783901868427222
1114	2	0.1801632147793093
1114	3	0.2510579926980583
1114	4	0.17269520586452458
1114	5	0.2089469064987597
1114	6	0.1967821231712108
1114	7	0.09251634721787028
1114	8	0.17553547131064207
1114	9	0.17534647181095764
1114	10	0.14963123604061224
1114	11	0.07587479402122099
1114	12	0.17748506550560705
1109	1	0.31509447646393696
1109	2	0.29457862454265893
1109	3	0.35324058163079286
1109	4	0.2795851620878338
1109	5	0.3309878241084654
1109	6	0.3682826317255774
1109	7	0.24970841750949901
1109	8	0.30265577075429206
1109	9	0.25775914123742183
1109	10	0.27487957587131717
1109	11	0.29774956488205007
1109	12	0.34798048224271483
1108	1	0.18354539605106623
1108	2	0.21304164295730388
1108	3	0.2154754386399773
1108	4	0.24223701097002603
1108	5	0.16172602192849794
1108	6	0.17088579203210585
1108	7	0.11090941600096826
1108	8	0.15239242052780355
1108	9	0.10800219320307747
1108	10	0.21035303645488312
1108	11	0.14188231155011977
1108	12	0.20758415651016193
1111	1	0.11063842751296349
1111	2	0.047140431958144546
1111	3	0.11715922525251443
1111	4	0.12552641080187854
1111	5	0.07233162838921103
1111	6	0.01694132538208848
1111	7	0.06881838146878039
1111	8	0.12214097084799391
1111	9	0.09965482268247783
1111	10	0.1216259836606497
1111	11	0.09841069993746743
1111	12	0.12298428039682108
1110	1	0.2724914982501936
1110	2	0.21558457545128107
1110	3	0.28377578615685195
1110	4	0.2090805272716949
1110	5	0.2758898731333552
1110	6	0.2636575538685939
1110	7	0.2306898659556751
1110	8	0.30877747047913573
1110	9	0.29620446091478314
1110	10	0.32093285153620243
1110	11	0.27307566842624453
1110	12	0.18754096304937917
1105	1	0.002699391640906157
1105	2	0.08083062159350973
1105	3	-0.356283778580579
1105	4	-0.04176129507801818
1105	5	0.09120737470144391
1105	6	0.10935516645284693
1105	7	0.24015180471937825
1105	8	0.26563561887148524
1105	9	0.27360284746159635
1105	10	0.27711186554486034
1105	11	0.28812490837929516
1105	12	0.19827883870373597
1104	1	-0.2066307316190077
1104	2	-0.2515308477626981
1104	3	-0.21871009681408893
1104	4	-0.19007192340199808
1104	5	-0.24849572211855175
1104	6	-0.18485810737335484
1104	7	-0.19246348545345882
1104	8	-0.3013582735149569
1104	9	-0.2528333323059293
1104	10	-0.14465730117456488
1104	11	-0.16933951065715291
1104	12	-0.21934543810317209
1107	1	-0.24987161476879294
1107	2	-0.13755071031566501
1107	3	-0.2569976538983205
1107	4	-0.22850808212804097
1107	5	-0.22188185621280354
1107	6	-0.16423677470936937
1107	7	-0.2851219468793005
1107	8	-0.2606059006126955
1107	9	-0.18502962067955764
1107	10	-0.22277415272721512
1107	11	-0.2243618072268987
1107	12	-0.28003338149959944
1106	1	-0.288946246592122
1106	2	-0.26645143555899864
1106	3	-0.3636921054002009
1106	4	-0.3221763518577916
1106	5	-0.25654376294844283
1106	6	-0.27805332112850323
1106	7	-0.2437726967827416
1106	8	-0.3126830653876276
1106	9	-0.24431514837257495
1106	10	-0.21562931742012026
1106	11	-0.24468646593697585
1106	12	-0.33725584171493495
2103	1	-0.2612499030942139
2103	2	-0.23444194356515524
2103	3	-0.30853443127001867
2103	4	-0.23552426883170696
2103	5	-0.2946525919650682
2103	6	-0.3645000051721965
2103	7	-0.34523210335479604
2103	8	-0.28321800561927546
2103	9	-0.26804429731556567
2103	10	-0.2685044864835824
2103	11	-0.26945922353298357
2103	12	-0.3072855015924034
2102	1	-0.07291244269338648
2102	2	-0.0365922531797942
2102	3	-0.022086036688875765
2102	4	-0.044189936825642206
2102	5	0.025058340895927955
2102	6	-0.020466271190592522
2102	7	-0.007655501874136661
2102	8	-0.054526843859353136
2102	9	-0.01927279836689877
2102	10	-0.05923026050304614
2102	11	-0.0514987019408224
2102	12	-0.045896153817150065
2101	1	-0.32269169282712085
2101	2	-0.19339714350734027
2101	3	-0.23753918873062574
2101	4	-0.23342477990643928
2101	5	-0.2481600331575109
2101	6	-0.2815562446384767
2101	7	-0.22374922935189545
2101	8	-0.1782231765621738
2101	9	-0.2652557592249387
2101	10	-1.229426394260937
2101	11	-0.5313677138985149
2101	12	-0.42148975138238143
2107	1	-0.18166103010653245
2107	2	-0.14201557466456194
2107	3	-0.12562630689158993
2107	4	-0.07571019219726693
2107	5	-0.1633804213999781
2107	6	-0.2133706558795545
2107	7	-0.1362338730456223
2107	8	-0.14146999505036106
2107	9	-0.07267187243809659
2107	10	-0.08819998427245324
2107	11	-0.1803614345882245
2107	12	-0.0809312816628669
2106	1	-0.04986098173176501
2106	2	-0.08621439063911751
2106	3	-0.09409925997537627
2106	4	-0.09149427795748011
2106	5	-0.03540818242026589
2106	6	-0.04286560673041606
2106	7	-0.03424032937556731
2106	8	-0.039893441082288206
2106	9	-0.05860342182333511
2106	10	-0.04793010951969734
2106	11	-0.17812823310809023
2106	12	-0.07304675941207606
2105	1	-0.11416478694747312
2105	2	-0.0645936879012794
2105	3	-0.07524264568867167
2105	4	-0.13664782914409557
2105	5	-0.09254219729266921
2105	6	-0.06203435618840558
2105	7	-0.04053541918982617
2105	8	-0.1277885202661615
2105	9	-0.16033943283356678
2105	10	-2.4397934862463444E-4
2105	11	-0.08819181428735234
2105	12	-0.06828191352729363
2104	1	0.005284378957522762
2104	2	-0.013941051688899364
2104	3	-0.03406079761290215
2104	4	-0.06689713280080767
2104	5	-0.045053858723068885
2104	6	1.29964340221278E-4
2104	7	0.04030293340865043
2104	8	-0.026973919638109578
2104	9	0.04716013862142887
2104	10	-0.002191666519276936
2104	11	-0.0476527554873627
2104	12	-0.03941597271837338
2111	1	-0.03504788772374212
2111	2	0.04230284205218737
2111	3	-0.09421581737072415
2111	4	-0.028291874372833092
2111	5	-0.08182331636103868
2111	6	-0.06753961724488633
2111	7	0.00633340492905532
2111	8	-0.04774025026720352
2111	9	-0.003152783086193267
2111	10	0.042620026055850246
2111	11	0.011891668548148715
2111	12	-0.09697278980011959
2110	1	0.21208195707011157
2110	2	0.18144417800855805
2110	3	0.2228806122784519
2110	4	0.14982046309849295
2110	5	0.17470724476261879
2110	6	0.11186943447070519
2110	7	0.1438315382762596
2110	8	0.061192620725321945
2110	9	0.10769412301091563
2110	10	0.18778189745144758
2110	11	0.14186818068062124
2110	12	0.1390603462703197
2109	1	0.07899382384513487
2109	2	0.04094404285519815
2109	3	0.08699697423016417
2109	4	0.07698155801222129
2109	5	0.12678692441873451
2109	6	0.04229321228393346
2109	7	0.15613218749476943
2109	8	0.10888648783841148
2109	9	0.031016158680202466
2109	10	0.1208081346008818
2109	11	0.06801860705328266
2109	12	0.07453267004890662
2108	1	0.07606782171122006
2108	2	0.0869533397887956
2108	3	0.17620833000950498
2108	4	0.10872931250625584
2108	5	0.06723980969473331
2108	6	0.13852683892479334
2108	7	0.12368012328822076
2108	8	0.11118650651076223
2108	9	0.09604727775325017
2108	10	0.21726989114274886
2108	11	0.11051144451282013
2108	12	0.0515761257757994
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	1.0
14	12.0
15	3377.0
16	160251.0
17	309507.0
18	317570.0
19	327018.0
20	258027.0
21	255750.0
22	216599.0
23	178593.0
24	163263.0
25	145715.0
26	129473.0
27	126416.0
28	123917.0
29	135783.0
30	149071.0
31	201193.0
32	292943.0
33	572117.0
34	1020673.0
35	1428139.0
36	152192.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.771681796053095	23.981169830574668	28.256787526782507	14.99036084658973
2	17.446520104163433	33.43620394687803	20.610972555488118	28.50630339347042
3	29.26540723279849	28.262677464556578	21.218117646247173	21.25379765639775
4	16.563786069950208	23.467139600455937	13.377557142000121	46.59151718759374
5	25.448092267082608	36.58395824584558	16.93766872637831	21.030280760693504
6	12.455471234027236	26.514113024176616	42.185059091727155	18.84535665006899
7	19.870838082668428	20.093106965024894	32.33209850620913	27.703956446097543
8	16.429197376746863	28.05194193592026	38.86512971160983	16.653730975723043
9	27.626327314176013	20.858224848521207	34.369908212850206	17.145539624452578
10	22.454706341112242	29.536819845221668	20.472763813066173	27.535710000599916
11	17.347668928500347	18.518990119231827	22.66850480960238	41.46483614266545
12	32.15084888115664	16.405663207151	27.640785290059394	23.80270262163297
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	5054.0
1	5054.0
2	5054.0
3	5054.0
4	17169.0
5	29284.0
6	29284.0
7	29284.0
8	29284.0
9	29284.0
10	29284.0
11	29284.0
12	29284.0
13	59596.5
14	89909.0
15	89909.0
16	89909.0
17	89909.0
18	89909.0
19	89909.0
20	89909.0
21	142753.0
22	195597.0
23	195597.0
24	195597.0
25	195597.0
26	195597.0
27	195597.0
28	195597.0
29	277905.5
30	360214.0
31	360214.0
32	360214.0
33	360214.0
34	360214.0
35	360214.0
36	360214.0
37	360214.0
38	901148.0
39	1442082.0
40	1442082.0
41	1442082.0
42	1442082.0
43	1442082.0
44	1442082.0
45	1442082.0
46	2324316.0
47	3206550.0
48	3206550.0
49	3206550.0
50	3206550.0
51	3206550.0
52	3206550.0
53	3206550.0
54	2161138.5
55	1115727.0
56	1115727.0
57	1115727.0
58	1115727.0
59	1115727.0
60	1115727.0
61	1115727.0
62	1115727.0
63	640562.0
64	165397.0
65	165397.0
66	165397.0
67	165397.0
68	165397.0
69	165397.0
70	165397.0
71	104207.5
72	43018.0
73	43018.0
74	43018.0
75	43018.0
76	43018.0
77	43018.0
78	43018.0
79	26360.5
80	9703.0
81	9703.0
82	9703.0
83	9703.0
84	9703.0
85	9703.0
86	9703.0
87	9703.0
88	6028.0
89	2353.0
90	2353.0
91	2353.0
92	2353.0
93	2353.0
94	2353.0
95	2353.0
96	2532.5
97	2712.0
98	2712.0
99	2712.0
100	2712.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	2.999580058791769E-5
2	1.4997900293958845E-5
3	1.4997900293958845E-5
4	0.0
5	0.0
6	0.0
7	0.0
8	1.4997900293958845E-5
9	0.0
10	0.0
11	1.4997900293958845E-5
12	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
12	6667600.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	16.388042090062918
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.6703851246747	12.728655405745656
2	12.058771400885977	3.952393065443328
3	3.8690226661352014	1.9021711890009336
4	1.9198819885301266	1.2585242734394162
5	0.9183984050485549	0.7525375858691185
6	0.6607327384893137	0.6496869557187245
7	0.4728420139148441	0.5424268378190605
8	0.3746001298101638	0.49111701554175974
9	0.2625089019349079	0.38718062405329223
>10	1.526570230122009	4.589292200743348
>50	0.13021770212427422	1.4757478127348966
>100	0.1116334370670814	3.6644428344490256
>500	0.011482728891404772	1.296466725788984
>1k	0.007459212564328254	2.3643980375633937
>5k	9.155533016183184E-4	1.153952329599287
>10k+	0.004577766505519867	62.79100710648976
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AGGCATCTTACG	726032	10.88895554622353	No Hit
GTCAATTGACCG	291446	4.37107804907313	No Hit
TCTAGCGTAGTG	250582	3.758203851460795	No Hit
GAACTAGTCACC	245359	3.679869818225449	No Hit
TAACGCTTGGGT	243215	3.647714319995201	No Hit
GAACCAAAGGAT	204775	3.0711950326954227	No Hit
GTACCTAATTGC	161943	2.4288049673045773	No Hit
GAGGCTCATCAT	161450	2.4214110024596556	No Hit
CCTCGTTCGACT	135428	2.0311356410102586	No Hit
GAGGAATAGCAG	128954	1.934039234507169	No Hit
ACACGTAAGCCT	124061	1.8606545083688284	No Hit
TACAGATGGCTC	118586	1.7785410042594036	No Hit
GCGATATATCGC	115556	1.7330973663687084	No Hit
TAGGAACTGGCC	104390	1.5656308116863638	No Hit
ATCCCGAATTTG	94114	1.4115123882656428	No Hit
TCACGGGAGTTG	91313	1.369503269542264	No Hit
TCGAGGACTGCA	78817	1.1820895074689544	No Hit
GCTGATGAGCTG	74376	1.115483832263483	No Hit
GAAGTTGGAAGT	74173	1.1124392585038094	No Hit
TAGGATTGCTCG	68373	1.025451436798848	No Hit
TCCAAAGTGTTC	53599	0.8038724578559001	No Hit
GTCTAATTCCGA	49057	0.7357519947207392	No Hit
CCTGAACTAGTT	45887	0.6882086507888896	No Hit
TGTAATTGTCGC	44564	0.6683664286999821	No Hit
ACGGGACATGCT	43304	0.6494690743295939	No Hit
ATTCCTGTGAGT	43135	0.6469344291799148	No Hit
GACTTTCCCTCG	35528	0.5328454016437699	No Hit
GCACACACGTTA	29685	0.44521267022616834	No Hit
GGCCACGTAGTA	27212	0.4081228627992081	No Hit
CTAACCTCCGCT	21816	0.3271941928130062	No Hit
GAGTGGTAGAGA	21178	0.31762553242546043	No Hit
GTTGGTCAATCT	20792	0.3118363429119923	No Hit
CCATGCGATAAC	20342	0.30508728777971084	No Hit
CACGGTTGTGAG	19429	0.29139420481132644	No Hit
TGTTATCGCACA	18990	0.2848101265822785	No Hit
GACTTGGTATTC	18101	0.2714769932209491	No Hit
TATCGTTGACCA	17122	0.25679404883316337	No Hit
GACGGAACCCAT	15261	0.22888295638610595	No Hit
TTACTGTGCGAT	14610	0.2191193232947387	No Hit
AAGAGATGTCGA	14247	0.21367508548803169	No Hit
CCATAGGGTTCA	13837	0.20752594636750857	No Hit
GGATCGCAGATC	13085	0.1962475253464515	No Hit
CTTCGGCAGAAT	12915	0.1936978822964785	No Hit
GTATGCGCTGTA	12421	0.1862889195512628	No Hit
AGGCTACACGAC	12365	0.18544903713480113	No Hit
ACGCGCAGATAC	11250	0.16872637830703702	No Hit
GCTCAGTGCAGA	10788	0.16179734837122803	No Hit
ACACCTGGTGAT	10766	0.16146739456476095	No Hit
CGACTGTCTTAA	10384	0.15573819665246866	No Hit
AGCTGGAAGTCC	10303	0.154523366728658	No Hit
AATCAGTCTCGT	9740	0.14607954886315916	No Hit
CTTGTGTCGATA	9688	0.14529965804787331	No Hit
CGGCATCTTACG	9235	0.13850560921470995	No Hit
CCAGTGTATGCA	8891	0.1333463315135881	No Hit
CAGTGCATATGC	8509	0.1276171336012958	No Hit
TTGCGCATACTA	8170	0.12253284540164376	No Hit
>>END_MODULE
>>Adapter Content	warn
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGCTTGG	65	0.001261617	6.0000005	4
GGCGTTG	275	0.0	6.0000005	6
CGGCATC	1145	0.0	6.0000005	1
CGCATGG	65	0.001261617	6.0000005	4
ACGGGCG	290	0.0	6.0000005	3
AACGCTG	130	3.6434358E-9	6.0000005	2
CGGGCGT	290	0.0	6.0000005	4
TTTGGGT	65	0.001261617	6.0000005	6
GCGTAGG	80	6.53666E-5	6.0000005	5
GTACGCC	235	0.0	6.0000005	5
GTGCCGA	80	6.53666E-5	6.0000005	6
CGTGGTG	55	0.009158845	6.0	6
GGAGCGC	75	1.7507214E-4	6.0	3
ATAGGGC	105	4.827416E-7	6.0	3
GCTGGGT	90	9.147643E-6	6.0	5
CATTGCC	55	0.009158845	6.0	4
CCATGCG	2520	0.0	6.0	1
GGCCACG	3010	0.0	6.0	1
AATGCCG	55	0.009158845	6.0	5
ACTAGGC	75	1.7507214E-4	6.0	3
>>END_MODULE