FastQCFastQC Report
Thu 3 Sep 2015
000000000-AHF14 l01n02.33200000034390.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AHF14 l01n02.33200000034390.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6667600
Sequences flagged as poor quality0
Sequence length12
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGCATCTTACG72603210.88895554622353No Hit
GTCAATTGACCG2914464.37107804907313No Hit
TCTAGCGTAGTG2505823.758203851460795No Hit
GAACTAGTCACC2453593.679869818225449No Hit
TAACGCTTGGGT2432153.647714319995201No Hit
GAACCAAAGGAT2047753.0711950326954227No Hit
GTACCTAATTGC1619432.4288049673045773No Hit
GAGGCTCATCAT1614502.4214110024596556No Hit
CCTCGTTCGACT1354282.0311356410102586No Hit
GAGGAATAGCAG1289541.934039234507169No Hit
ACACGTAAGCCT1240611.8606545083688284No Hit
TACAGATGGCTC1185861.7785410042594036No Hit
GCGATATATCGC1155561.7330973663687084No Hit
TAGGAACTGGCC1043901.5656308116863638No Hit
ATCCCGAATTTG941141.4115123882656428No Hit
TCACGGGAGTTG913131.369503269542264No Hit
TCGAGGACTGCA788171.1820895074689544No Hit
GCTGATGAGCTG743761.115483832263483No Hit
GAAGTTGGAAGT741731.1124392585038094No Hit
TAGGATTGCTCG683731.025451436798848No Hit
TCCAAAGTGTTC535990.8038724578559001No Hit
GTCTAATTCCGA490570.7357519947207392No Hit
CCTGAACTAGTT458870.6882086507888896No Hit
TGTAATTGTCGC445640.6683664286999821No Hit
ACGGGACATGCT433040.6494690743295939No Hit
ATTCCTGTGAGT431350.6469344291799148No Hit
GACTTTCCCTCG355280.5328454016437699No Hit
GCACACACGTTA296850.44521267022616834No Hit
GGCCACGTAGTA272120.4081228627992081No Hit
CTAACCTCCGCT218160.3271941928130062No Hit
GAGTGGTAGAGA211780.31762553242546043No Hit
GTTGGTCAATCT207920.3118363429119923No Hit
CCATGCGATAAC203420.30508728777971084No Hit
CACGGTTGTGAG194290.29139420481132644No Hit
TGTTATCGCACA189900.2848101265822785No Hit
GACTTGGTATTC181010.2714769932209491No Hit
TATCGTTGACCA171220.25679404883316337No Hit
GACGGAACCCAT152610.22888295638610595No Hit
TTACTGTGCGAT146100.2191193232947387No Hit
AAGAGATGTCGA142470.21367508548803169No Hit
CCATAGGGTTCA138370.20752594636750857No Hit
GGATCGCAGATC130850.1962475253464515No Hit
CTTCGGCAGAAT129150.1936978822964785No Hit
GTATGCGCTGTA124210.1862889195512628No Hit
AGGCTACACGAC123650.18544903713480113No Hit
ACGCGCAGATAC112500.16872637830703702No Hit
GCTCAGTGCAGA107880.16179734837122803No Hit
ACACCTGGTGAT107660.16146739456476095No Hit
CGACTGTCTTAA103840.15573819665246866No Hit
AGCTGGAAGTCC103030.154523366728658No Hit
AATCAGTCTCGT97400.14607954886315916No Hit
CTTGTGTCGATA96880.14529965804787331No Hit
CGGCATCTTACG92350.13850560921470995No Hit
CCAGTGTATGCA88910.1333463315135881No Hit
CAGTGCATATGC85090.1276171336012958No Hit
TTGCGCATACTA81700.12253284540164376No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCTTGG650.0012616176.00000054
GGCGTTG2750.06.00000056
CGGCATC11450.06.00000051
CGCATGG650.0012616176.00000054
ACGGGCG2900.06.00000053
AACGCTG1303.6434358E-96.00000052
CGGGCGT2900.06.00000054
TTTGGGT650.0012616176.00000056
GCGTAGG806.53666E-56.00000055
GTACGCC2350.06.00000055
GTGCCGA806.53666E-56.00000056
CGTGGTG550.0091588456.06
GGAGCGC751.7507214E-46.03
ATAGGGC1054.827416E-76.03
GCTGGGT909.147643E-66.05
CATTGCC550.0091588456.04
CCATGCG25200.06.01
GGCCACG30100.06.01
AATGCCG550.0091588456.05
ACTAGGC751.7507214E-46.03
Produced by FastQC (version 0.11.2)