FastQCFastQC Report
Thu 3 Sep 2015
000000000-AHF14 l01n01.33100000034393.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AHF14 l01n01.33100000034393.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6667600
Sequences flagged as poor quality0
Sequence length251
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACGTAGGTGGCAAGCGTTATCCGGAATTATTGGGCGTAAAGCGCGCGTA157838423.672445857579937No Hit
TACGTAGGGTGCGAGCGTTGTCCGGAATTACTGGGCGTAAAGGGCTCGTA3460305.189723438718579No Hit
TACGTAGGGTGCGAGCGTTAATCGGAATTATTGGGCGTAAAGCGAGTGTA2716864.074719539264502No Hit
TACGTAGGTCCCGAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGCGCA2382513.5732647429359887No Hit
TACGTATGGAGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGGGTACGCA1760402.6402303677485155No Hit
CACGATTAACCCAAGTCAATAGAAGCCGGCGTAAAGAGTGTTTTAGATCA1699132.5483382326474295No Hit
TACGTAGGTGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGCGCGCGCA1282151.9229557861899333No Hit
TACGAAGGGTGCAAGCGTTACTCGGAATTACTGGGCGTAAAGCGTGCGTA715081.0724698542204092No Hit
TACGTAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGAGCGCA692931.0392495050692903No Hit
TACGTAGGGCGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGAGCTTGTA616510.9246355510228568No Hit
TACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCA599440.899034135221069No Hit
TACGGAAGGTCCAGGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA543480.8151058851760753No Hit
TACGTAGGTCCCGAGCGTTATCCGGATTTATTGGGCGTAAAGCGAGCGCA527120.7905693202951586No Hit
TACGTAGGGTGCGAGCGTTGTCCGGAATTACTGGGCGTAAAGAGCTCGTA509950.7648179254904314No Hit
TACGTAGGGTGCGAGCGTTGTCCGGATTTATTGGGCGTAAAGGGCTCGTA379190.5687053812466255No Hit
TACGTAGGGGGCTAGCGTTGTCCGGAATCACTGGGCGTAAAGGGTTCGCA338420.5075589417481553No Hit
TACGTAGGGCGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGGGCTTGTA285230.42778511008458814No Hit
TACAGAGGATGCAAGCGTTATCCGGAATGATTGGGCGTAAAGCGTCTGTA278580.4178115063891055No Hit
TACGTAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGTGCGCA276180.41421201031855537No Hit
GACAGAGGATGCAAGCGTTATCCGGAATGATTGGGCGTAAAGCGTCTGTA268400.4025436438898554No Hit
TACGTAGGTGGCAAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGCGCA234850.3522256884036235No Hit
CACGATTAACCCAAGTCAATAGAAGCCGGCGTAAAGAGTGTTTTTGATCA203580.3053272541844142No Hit
TACGTAAGGACCGAGCGTTGTCCGGAATCATTGGGCGTAAAGGGTACGTA201800.3026576279320895No Hit
TACGTAGGGCGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGAGCTCGTA197520.29623852660627514No Hit
TACGTAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGTGCGCA191190.2867448557201992No Hit
TACGGAGGATGCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTA187700.2815105885176075No Hit
TACGTAGGGGGCAAGCGTTGTCCGGATTCACTGGGCGTAAAGCGCGCGCA178940.26837242786009957No Hit
TACGTAGGTGGCAAGCGTTATCCGGCATTATTGGGCGTAAAGCGCGCGTA166330.2494600755894175No Hit
TACGTAGGTGGCAAGCGTTATCCGGATTTATTGGGCGTAAAGCGAGCGCA156390.2345521626972224No Hit
TACGAAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCGCGTA151760.22760813486111942No Hit
CACGATTAACCCAAGTCAATAGAAGCCGGCGGAAAGAGTGTTTTAGATCA148880.22328873957645928No Hit
TACAGAGGGTGCGAGCGTTAATCGGATTTACTGGGCGTAAAGCGTGCGTA145360.21800947867298578No Hit
TACGTAGGGCGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGAGCTCGTA143140.21467994480772692No Hit
CACGATTAACCCAAGTCAATAGAAGCCGGCGTAAAAAGTGTTTTAGATCA119350.17899994000839883No Hit
TACAGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCGCGTA104020.1560081588577599No Hit
TACGTAGGTGGCGAGCGTTATCCGGAATTATTGGGCGTAAAGGGTGCGTA102410.15359349691043253No Hit
TACGTATGGACCGAGCGTTGTCCGGAATCATTGGGCGTAAAGGGTACGTA101980.1529485871977923No Hit
TACGGAGGGTGCGAGCGTTAATCGGAATAACTGGGCGTAAAGGGCACGCA100430.1506239126522287No Hit
TACGTAGGGTGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGAGCTCGTA88130.1321764952906593No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGCA84120.1261623372727818No Hit
TACGTAGGGTGCGAGCGTTATCCGGAATTATTGGGCGTAAAGAGCTCGTA82860.124272601835743No Hit
TACAGAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGAGTGTA74360.11152438658587797No Hit
TACGTATGTCGCGAGCGTTATCCGGAATTATTGGGCATAAAGGGCATCTA73700.11053452516647669No Hit
CACGATTAACCCAAGTCAATAGAAGCCGGCGTAAAGAGTGGTTTAGATCA71500.10723498710180573No Hit
TACGTAGGGCGCAAGCGTTATCCGGAATTATTGGGCGTAAAGAGCTCGTA67260.10087587737716719No Hit
TACGGAAGGTCCGGGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA67190.10077089207510949No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGTCC324800.0244.622855
GTGCGAG976100.0244.410169
GTAGGTC328900.0244.404074
GGTCCCG326050.0244.361287
TAGGGTG978300.0244.211145
GTCCCGA328000.0244.103658
TACGTAT248300.0244.062621
TAGGGCG173100.0244.009255
ACCCAAG302100.0242.648139
CGTAGGG1255100.0242.403813
GTAGGGT998150.0242.017724
AGGTCCC328900.0241.945886
GTAGGGC175550.0241.859864
AGGATGC100100.0241.818186
GGGTGCA268250.0240.890037
ACGTATG251750.0240.766622
ATGGAGC201100.0240.675036
ACGTAGG3578300.0240.094242
TCAAACA1570900.0240.07161245
TCCCGAG333750.0240.008249
Produced by FastQC (version 0.11.2)