FastQCFastQC Report
Tue 22 Mar 2016
000000000-AHF0P_l01n03.3330000004df1c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AHF0P_l01n03.3330000004df1c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13661022
Sequences flagged as poor quality0
Sequence length251
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTTTGCTCCCCACGCTTTCGCACCTGAGCGTCAGTCTTCGTCCAGGG143157310.479252577149792No Hit
CCTGTTTGCTCCCCACGCTTTCGCACATGAGCGTCAGTATATTCCCAAGG8886146.50474027492233No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGTCCAGAG8826436.461031978427383No Hit
CCTGTTTGCTCCCCACGCTTTCGCACATGAGCGTCAGTACATTCCCAAGG4342223.1785469637630333No Hit
CCTGTTCGCTCCCCACGCTTTCGTCCATCAGCGTCAGTTGTAACTTGGTA4050482.964990467038264No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTTACAGTCCAGAG3631232.658095419215341No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTAATGTCCAGCA2947982.1579498224949787No Hit
CCTGTTTGATACCCACGCTTTCGTGCTTCAGTGTCAGTTGCGGTTTGGTA2910672.130638542270117No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG2528971.8512304569892355No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTCATCGTCCAGAA2505471.8340282300987436No Hit
CCTGTTCGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGTCCAGAG1848471.353097886819888No Hit
CCTGTTCGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTTACAGTCCAGAA1843481.3494451586418643No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTCATCGTCCAGCA1759191.2877440648291174No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTGAGCGTCAGTCTTTGTCCAGGG1388021.016044041214486No Hit
CCTGTTTGCTACCCACGCTTTCGCGCTTCAGCGTCAGTATCTGTCCAGTA1275180.9334440717539287No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGTGTCAGTTGCAGTCCAGTG1256190.9195432084071016No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTTACAGTCCAGAA1252040.9165053683392063No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTTTCAGTCCAGAA1203270.8808052574690238No Hit
CCTGTTCGCTCCCCACGCTTTCGTCCATCAGCGTCAATAATTTGTTAGTA1116970.8176328242498988No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGTCCAGAA1041130.7621172120211797No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTTACAGTCCAGAG1039250.7607410338699403No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTACAGTCCAGAG838050.613460691301134No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTTATGGTCCAGAA759730.5561296951282269No Hit
CCTGTTCGCTCCCCATGCTTTCGCTCCTCAGCGTCAGTTACTGCCCAGAG746900.5467380112556732No Hit
CCTGTTCGCTCCCCACGCTTTCGTGACTCAGCGTCAGTTTCAGTCCAGTA741870.543056002691453No Hit
CCTGTTTGCTACCCACGCTTTCGCGCTTTAGCGTCAGTATCTGTCCAGTA686270.5023562658782044No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTATTGGCCCAGGG615050.45022253825519054No Hit
CCTGTTCGCTCCCCTAGCTTTCGCACTTCAGCGTCAGTTATCGTCCAGTG606070.44364909155405796No Hit
CCTGTTTGCTACCCACGCTTTCGAACCTCAGTGTCAGTATGATGCCAGGA583960.4274643580838974No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTGATCGTCCAGTA555500.40663136330502947No Hit
CCTGTTTGCTACCCACGCTTTCGCACATGAGCGTCAGTATTGCCCCAGAA549160.4019904220928712No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTGTTATCCCAGGA495710.3628645060376888No Hit
CCTGTTTGATCCCCACGCTTTCGCACATCAGCGTCAGTTACAGACCAGAA493770.3614444073071546No Hit
CCTGTTCGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG440310.32231117115542307No Hit
CCTGTTTGCTACCCACGCTTTCGAGCATGAACGTCAGTATTATCCCAGGG405240.29663959255757No Hit
CCTGTTTGCTACCCACGCTTTCGTGCCTCAGTGTCAGTTGCAGTCCAGAA395780.28971478122207844No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTACAGGTCCAGGG368070.2694307936843964No Hit
CCCGTTCGCTCCCCTGGCTTTCGCGCCTCAGCGTCAGTTGTCGTCCAGAA352410.2579675224884346No Hit
CCTGTTTGATCCCCACGCTTTCGCACATCAGCGTCAGTTGCAGACCAGAA346580.2536999062002828No Hit
CCTGTTTGCTACCCACGCTTTCGTGCCTCAGCGTCAGTTTTAGTCCAGAA346240.2534510229176119No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTGCTGGCCCAGAT337240.24686293602338097No Hit
CCTGTTTGCTACCCACGCTTTCGAATCTCAGTGTCAATATTATGCCAGGA335140.24532571574806045No Hit
CCTGTTCGCTACCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG324140.23727360954400042No Hit
CCTGTTTGATACCCACGCTTTCGTGCTTCAGTGTCAGTTGTACCTTAGTA322460.236043833323744No Hit
CCTGTTCGCTCCCCACGCTTTCGTCCCTCAGCGTCAGTTATATCTTGGTA289890.2122022788631773No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTATCTGTCCAGAA280930.205643472355143No Hit
CCTGTTTGCTACCCACGCTTTCGGGCATGAACGTCAGTATTATCCCAGGG279350.204486897100378No Hit
CCGGTTTGCTCCCCACACTTTCGCGCCTCAGCGTCACCTTCTGTCCAGTA262090.19185241045655296No Hit
CCTGTTTGCTCCCCACGCTTTCGCACATGAGCGTCAGTATCTTCCCAAGG254750.1864794595894802No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTAACGGCCCAGAG251510.18410774830755708No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTTAGCGTCAGTCTTCGTCCAGGG241550.17681693214460822No Hit
CCTGTTCGCTCCCCATGCTTTCGCTCCTCAGCGTCAGTAACAGCCCAGAG232150.1699360413884115No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTGATGGTCCAGTT231720.16962127723679823No Hit
CCTGTTTGATACCCGCACTTTCGAGCATCAGTGTCAGTGCCGGCCCGGTG231340.16934311356793072No Hit
CCTGTTCGCTCCCCATGCTTTCGCTCCTCAGCGTCAGTAACGGCCCAGAG221600.16221333952906306No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGCACAGGCCCAGAG219330.16055167761240705No Hit
CCTGTTTGCTCCCCACGCTTTCACGCATTAGCGTCAGTTAAGTTCCAGCA208970.15296805758749235No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGTGTCAGTATCAGTCCAGGT198060.14498183225237468No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTACAGGCCCAGGG190930.13976260341283397No Hit
CCTGTTTGATACCCACGCTTTCGTGCTTCAGTGTCAGTTATACCTTAGTA189070.1384010654546929No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAACGTCAGTCATCGTCCAGAA184940.13537786557989584No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAAGCCAGAG180780.13233270541545136No Hit
CCTGTTCGCTCCCCACGCTTTCGCTTCTCAGCGTCAGTAACAGTCCAGAG176980.12955106872677608No Hit
CCTGTTTGCTCCCCACGCTTTCGTTCCTCAGTGTCAATATACAACCAGTT176840.12944858737508805No Hit
CCTGTTTGATACCCACGCTTTCGTGCATCAGCGTCAGTTGTAGCCTAGTA171150.12528345243862427No Hit
CCTGTTTGCTCCCCATGCTTTCGTACCTCAGCGTCAGTATTAGGCCAGAT167780.12281657990156228No Hit
CCTGTTCGCTACCCATGCTTTCGCTCCTCAGCGTCAGTAACTGCCCAGTA164540.12044486861963914No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTGAGCGTCAGTCTTCGTCCCGGG155700.11397390327019458No Hit
CCTGTTCGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTACAGTCCAGAG152610.11171199343650863No Hit
CCTGTTTGCTCCCCACGCTCTCGCACCTGAGCGTCAGTCTTCGTCCAGGG149380.10934760225113464No Hit
CCTGTTCGCTCCCCATGCTTTCGCTCCTCAGCGTCAGTTACAGCCCAGAG143020.10469202084587816No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAGTATCGAGCCAGTG140650.10295715796373069No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTACAGACCAGAG138200.10116373430919005No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTGCTC8067250.0244.274155
TGTTTGC8693250.0244.267263
GTTTGCT8776050.0243.174244
CTGTTCG1874000.0242.823262
CTGTTTG9535100.0242.445972
CTCCCCA9802350.0242.223169
TGTTTGA788550.0241.799823
TTGATAC657100.0241.36476
CCTGTTC1866400.0240.69441
CCTGTTT9540450.0239.711171
GCTCCCC10165450.0239.683278
TGTTCGC1889150.0238.943573
TTGCTCC8436000.0236.14946
TGCTCCC8518300.0233.900887
GTTTGAT831200.0229.76124
TTTGATC147550.0229.059655
GATACCC709950.0227.296658
GTTCGCT2051050.0227.09434
TTTGCTA768600.0225.810565
TGATACC705100.0224.690467
Produced by FastQC (version 0.11.2)