FastQCFastQC Report
Tue 22 Mar 2016
000000000-AHF0P_l01n02.3320000004df1f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AHF0P_l01n02.3320000004df1f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13661022
Sequences flagged as poor quality0
Sequence length12
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACGTGGGATC6867415.027010424256692No Hit
TATGCACCAGTG3106582.2740465537644257No Hit
ACACTAGATCCG2901722.124087055858632No Hit
TCCGAATTCACA2608581.9095057456169822No Hit
TGGCAAGACTCT2586981.8936943370708281No Hit
CCTCTCGTGATC2506071.8344674358916926No Hit
TCTGTTGCTCTC2465741.804945486508989No Hit
GACAGGAGATAG2431181.7796472328351423No Hit
TGCAATGTTGCT2287551.6745086860997662No Hit
AATGGAGCATGA2192221.6047262056967628No Hit
TAATCCACAGCG2183521.598357721699006No Hit
AGCTGTTGTTTG2142531.5683526459440589No Hit
ATGAGACTCCAC2088601.5288753652545177No Hit
CCATGCGATAAC2083641.5252445973661413No Hit
TACTAATCTGCG2079741.5223897597119747No Hit
GTACATACCGGT2062911.5100700372197629No Hit
AATCTTGCTGCA1967101.4399361921824003No Hit
GCTCGAAGATCG1779671.3027356225617674No Hit
TCGGAGTGTTGT1758791.2874512609671516No Hit
AGCTTGACAGCT1751721.2822759527069059No Hit
CAGCTAGAACGC1719411.258624720756617No Hit
GAGGAATAGCAG1691331.2380698896466165No Hit
TAAACCGCGTGT1683471.2323162937589882No Hit
GGTCACTGACAG1674761.2259404896646824No Hit
TATCGTTGACCA1631501.1942737519930793No Hit
GGATCGCAGATC1610571.178952789915718No Hit
ATGTCGAGAGAA1600101.1712886488287626No Hit
GAACTAGTCACC1459821.0686023344373503No Hit
TCGAGGACTGCA1375011.006520595604048No Hit
CCATAGGGTTCA1292330.9459980373357133No Hit
GAGTGGTAGAGA1255760.9192284442554883No Hit
GAATCTTCGAGC1244000.9106200107136933No Hit
CGATCCGTATTA1225320.8969460703598896No Hit
GCCTGAATTTAC1191070.8718747396790665No Hit
GCTGATGAGCTG1165100.8528644489409357No Hit
GAACCAAAGGAT1150720.8423381501032646No Hit
ATGGTTGTTGGC1124000.822778852123948No Hit
ACACGTAAGCCT1116900.8175815835740547No Hit
GACTTTCCCTCG1080850.7911926355143853No Hit
GCACACACGTTA1076430.787957152839663No Hit
TCCTCTGTCGAC1041410.7623221747245558No Hit
AACACAAGGAGT1023000.748845876977579No Hit
GACACATTTCTG1010650.7398055577393844No Hit
CACGGTTGTGAG938970.6873351056751098No Hit
AATCAGTCTCGT858260.628254606426957No Hit
GTATGCGCTGTA820090.6003137978988687No Hit
CCTGAACTAGTT801860.5869692618897766No Hit
CTATTTGCGACA785300.5748471820043918No Hit
AGGCATCTTACG744810.5452081110769018No Hit
GACGGAACCCAT690620.5055405078770827No Hit
ACTCACGGTATG662150.48470019300166556No Hit
TCAACAGCATCG595380.4358239083430215No Hit
CGGAGCTATGGT570950.41794091247345916No Hit
TTACTGTGCGAT567150.4151592757847839No Hit
CTAGCGAACATC548580.4015658564930208No Hit
GGAAACCACCAC545170.39906970356976224No Hit
CTGCTAACGCAA533870.3907979944692278No Hit
CAAGCATGCCTA529400.38752591131175984No Hit
TCACGGGAGTTG525290.38451735163006107No Hit
AGCAAACACCCG519950.3806084200728174No Hit
ATTCCTGTGAGT518790.37975928887311655No Hit
ACCACATACATC498000.36454080814744316No Hit
TAACACCACATC485120.3551125237921438No Hit
AATGTCCGTGAC480330.35160619754510314No Hit
TCCCAGAACAAC473230.34640892899520986No Hit
AAGGAGCGCCTT451910.33080248315243177No Hit
GATTGGTTGCAC433640.31742866675714304No Hit
GACTCTTGGCAA430030.31478611190290157No Hit
ATGGACCGAACC396800.2904614310700912No Hit
ATGTGGGACCCA394580.2888363696361809No Hit
CGCAGCGGTATA391980.28693314453340313No Hit
TAGCTCGTAACT384950.2817871166593539No Hit
TTGCGCATACTA383350.2806159012114906No Hit
AGGCTACACGAC373680.27353736784846694No Hit
CTAACCTCCGCT368550.2697821583187554No Hit
GGTGTCTATTGT364120.2665393555474839No Hit
ATCGATCTGTGG361250.2644384878378792No Hit
TAACGCTTGGGT358300.26227905935588125No Hit
CCACCTACTCCA320990.23496777913101963No Hit
CCTCGTTCGACT316790.23189333858037853No Hit
TTATGCAGTCGT309110.22627150443063482No Hit
GACTCGAATCGT306280.22419991710722667No Hit
AGCTGGAAGTCC296800.21726046557863676No Hit
TAGGATTGCTCG286530.20974272642266442No Hit
AGATGTTCTGCT267320.19568082095175604No Hit
ACGCGCAGATAC258400.18915129482991827No Hit
TCAAGCTCAAGC257290.18833876411296313No Hit
ACGTGTACCCAA243990.1786030357025997No Hit
GTCAATTGACCG241030.17643628712405265No Hit
ACATTCAGCGCA223600.16367735883889215No Hit
TACACGATCTAC222500.16287214821848614No Hit
GTTGGTCAATCT213590.15634994219319756No Hit
GCGGCAATTACG196160.14359101390803702No Hit
GCACGACAACAC192240.140721536060772No Hit
ATCGGCGTTACA191180.13994560582656262No Hit
GTCTAATTCCGA184380.1349679401731437No Hit
ACAAGGAGGTGA180390.13204722165003469No Hit
ATTAGTTCGCGT170950.12513705050764137No Hit
AGAGCCTACGTT155900.11412030520117748No Hit
TGTAATTGTCGC152440.11158755179517316No Hit
ATACTTCGCAGG149700.1095818453407073No Hit
GTACCTAATTGC147190.1077445011068718No Hit
GCTCAGTGCAGA137680.10078308928863448No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGTCA550.0091605056.00000055
GTCACCG650.00126193226.00000052
CGGCTGT806.539086E-56.00000053
GTACGGG2600.06.00000051
CTGCTAA56900.06.01
AGCCGGC2400.06.01
CATGCGG852.4448516E-56.02
CGCGGAC1202.5691406E-86.02
GGGTCAA751.751294E-46.06
ACGGGCG751.751294E-46.03
GCTTCGG2650.06.01
ACGTGGC1800.06.03
GGCCTGC704.6972226E-46.05
GTACGTG714100.05.9970591
TGGCAAG270050.05.99666741
TGCAATG233600.05.99486351
CAGCTAG175250.05.99315261
TAAACCG174900.05.9931391
GACAGGA254650.05.9929321
GCCTGAA117750.05.9923571
Produced by FastQC (version 0.11.2)