##FastQC	0.11.2
>>Basic Statistics	pass
#Measure	Value
Filename	000000000-AHBE6 l01n01 150915_gms8.341000000347fb.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2917158
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.94455939650852	33.0	32.0	34.0	32.0	34.0
2	33.2558404447068	34.0	33.0	34.0	32.0	35.0
3	33.425059595674966	34.0	33.0	34.0	32.0	35.0
4	33.496476365010054	34.0	33.0	34.0	32.0	35.0
5	32.80823870356011	34.0	33.0	34.0	32.0	35.0
6	35.80870079714572	37.0	35.0	37.0	33.0	37.0
7	35.75621683844344	37.0	35.0	37.0	33.0	37.0
8	35.78443334231468	37.0	35.0	37.0	33.0	37.0
9	36.03591680670022	37.0	37.0	37.0	33.0	37.0
10	36.12210514480189	37.0	37.0	37.0	34.0	37.0
11	36.00873007221412	37.0	37.0	37.0	33.0	37.0
12	36.08807236358127	37.0	37.0	37.0	34.0	37.0
13	37.503952477034154	38.0	38.0	38.0	37.0	38.0
14	37.53590857951472	38.0	38.0	38.0	37.0	38.0
15	37.4893512795673	38.0	38.0	38.0	37.0	38.0
16	37.46635766729125	38.0	38.0	38.0	37.0	38.0
17	37.48502515119167	38.0	38.0	38.0	37.0	38.0
18	37.442988346877335	38.0	38.0	38.0	37.0	38.0
19	37.473614387702	38.0	38.0	38.0	37.0	38.0
20	37.455539946756396	38.0	38.0	38.0	37.0	38.0
21	37.47115377363859	38.0	38.0	38.0	37.0	38.0
22	37.49915499948923	38.0	38.0	38.0	37.0	38.0
23	38.11692167513724	39.0	38.0	39.0	37.0	39.0
24	38.01884711078385	39.0	38.0	39.0	37.0	39.0
25	38.05396142409839	39.0	38.0	39.0	37.0	39.0
26	37.91240789837232	38.0	38.0	39.0	37.0	39.0
27	37.74047857538056	38.0	38.0	39.0	37.0	39.0
28	37.31605144459093	38.0	38.0	39.0	36.0	39.0
29	37.572555206128705	38.0	38.0	39.0	36.0	39.0
30	37.61628852465311	38.0	38.0	39.0	36.0	39.0
31	37.74228684219367	38.0	38.0	39.0	37.0	39.0
32	37.79705315927351	38.0	38.0	39.0	37.0	39.0
33	37.858907539461356	39.0	38.0	39.0	37.0	39.0
34	37.93081759712707	39.0	38.0	39.0	37.0	39.0
35	37.842697927229175	38.0	38.0	39.0	37.0	39.0
36	37.03442254413371	38.0	38.0	39.0	35.0	39.0
37	37.335724701918785	38.0	38.0	39.0	36.0	39.0
38	37.59008425323551	38.0	38.0	39.0	36.0	39.0
39	37.92898019236531	39.0	38.0	39.0	37.0	39.0
40	38.06551239254096	39.0	38.0	39.0	37.0	39.0
41	38.13869492156407	39.0	38.0	39.0	37.0	39.0
42	38.00035753976987	39.0	38.0	39.0	37.0	39.0
43	38.08696375033509	39.0	39.0	39.0	37.0	39.0
44	37.817154915846174	39.0	38.0	39.0	37.0	39.0
45	38.02278450464459	39.0	38.0	39.0	37.0	39.0
46	37.94750507171706	39.0	38.0	39.0	37.0	39.0
47	37.94290676062113	39.0	38.0	39.0	37.0	39.0
48	37.938128822641765	39.0	38.0	39.0	37.0	39.0
49	38.015133907728	39.0	38.0	39.0	37.0	39.0
50	37.628880232061476	39.0	38.0	39.0	37.0	39.0
51	37.82118006635225	39.0	38.0	39.0	37.0	39.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	-0.1176357172258804
1101	2	0.08084667564218506
1101	3	0.17754603877901332
1101	4	0.18681490203898932
1101	5	0.042312835405901694
1101	6	0.08681979266641804
1101	7	0.07680333694988661
1101	8	0.12521235990239887
1101	9	0.0844585623411831
1101	10	0.018904385080112718
1101	11	0.09526560831864117
1101	12	0.05127374858276568
1101	13	0.033820674397496475
1101	14	0.04326010223019239
1101	15	0.024978634035051073
1101	16	0.05182232976472534
1101	17	-0.05930265425289605
1101	18	0.036823024296644746
1101	19	0.04542985666319055
1101	20	0.03537537054198481
1101	21	0.033766160623542874
1101	22	0.05819377843405249
1101	23	0.12396596284819594
1101	24	0.08177046519798381
1101	25	0.06131182728183404
1101	26	0.1010757739294661
1101	27	0.09603757229386645
1101	28	0.44043232171519975
1101	29	0.19455144359655918
1101	30	0.20762799763380002
1101	31	0.10068881870630264
1101	32	0.14212534666860677
1101	33	0.12689420682681174
1101	34	0.07344647046375741
1101	35	0.13166463645196558
1101	36	0.7839652623961726
1101	37	0.5246755385541775
1101	38	0.23705295777318014
1101	39	0.17335360892784735
1101	40	0.09177869714397957
1101	41	0.04772275437576923
1101	42	0.18427422984929365
1101	43	0.1775550286821712
1101	44	0.40521896692693105
1101	45	0.1688189542324352
1101	46	0.14799121742262855
1101	47	0.1313845350927636
1101	48	0.14093294686034596
1101	49	0.030007241798180928
1101	50	0.46578167059106335
1101	51	0.09781283829769905
1102	1	-0.0848359804338017
1102	2	0.08233010513720984
1102	3	0.040330949325031895
1102	4	0.10805717577775908
1102	5	0.03468297100175022
1102	6	0.0029029053786260306
1102	7	0.11237130589163513
1102	8	0.08135452618389394
1102	9	0.008663082781673381
1102	10	-0.06799275492128487
1102	11	-0.0357743324704245
1102	12	0.08524426502702198
1102	13	0.0587125252798657
1102	14	0.02986625205649318
1102	15	0.08854043480987883
1102	16	0.06447878355891135
1102	17	-0.022829817646737638
1102	18	0.036236648706967856
1102	19	0.060192778712441
1102	20	0.08434537420829713
1102	21	0.17866857816803616
1102	22	0.12041788898137895
1102	23	0.07308237501602832
1102	24	0.03153126830770958
1102	25	0.08032586835362565
1102	26	0.13726041486462748
1102	27	0.13970932278751036
1102	28	0.4584139336973081
1102	29	0.18835817135162358
1102	30	0.24806280800272873
1102	31	0.16127590151351257
1102	32	0.20249847355952966
1102	33	0.2261491685185888
1102	34	0.16174996048285806
1102	35	0.18228805504751477
1102	36	0.8412753957383359
1102	37	0.5906694622712649
1102	38	0.38729535613417454
1102	39	0.2198523485070183
1102	40	0.22425680369651957
1102	41	0.21311697208554392
1102	42	0.2956501948349697
1102	43	0.22998427572700564
1102	44	0.41565375378223024
1102	45	0.23295077053892044
1102	46	0.1617931827639012
1102	47	0.18091302356990724
1102	48	0.19558063224222622
1102	49	0.17003058178587338
1102	50	0.5638736754334204
1102	51	0.17806774048192153
1103	1	0.11265569641567197
1103	2	0.09338439335157034
1103	3	-0.025603639302644865
1103	4	0.03157674080340911
1103	5	-0.07074598085195305
1103	6	0.08441600192076493
1103	7	-0.5595003504137352
1103	8	-0.9194898711164896
1103	9	-0.8150282905080743
1103	10	-0.22803792110352816
1103	11	-0.11025119162099628
1103	12	0.03677186920981512
1103	13	0.09488608680205601
1103	14	0.1247588500930803
1103	15	0.12836056227396142
1103	16	0.012224960808268293
1103	17	0.03799479047761167
1103	18	0.019607730448811367
1103	19	0.15565805854196668
1103	20	0.20265249344151215
1103	21	0.19888185322935215
1103	22	0.1081593909503269
1103	23	0.08989314182125696
1103	24	-0.20689962978470078
1103	25	0.088230304235978
1103	26	0.05581109247101068
1103	27	0.15150625471998325
1103	28	0.4206693445602596
1103	29	0.129975257949539
1103	30	0.19637960831191492
1103	31	0.13270654551541128
1103	32	0.20530829025477715
1103	33	0.1126756006208467
1103	34	0.14695702226903506
1103	35	0.18587491772942144
1103	36	0.838498462879528
1103	37	0.6267919001406597
1103	38	0.3798342651651083
1103	39	0.1830794627635015
1103	40	0.1471279236879397
1103	41	0.1815369192083338
1103	42	0.2863140731013516
1103	43	0.11986432816079429
1103	44	0.371687654576931
1103	45	0.14635141084404069
1103	46	0.10764662973333827
1103	47	0.10150733379799703
1103	48	0.09392401536038619
1103	49	0.19162963574490988
1103	50	0.47726567092261973
1103	51	0.31715843179257064
2112	1	-0.12318859976414132
2112	2	0.08597517022224821
2112	3	0.047796512660234214
2112	4	0.11187434512535788
2112	5	0.17821942398283142
2112	6	0.1471872795432816
2112	7	-0.4910860756668427
2112	8	0.018180277775194043
2112	9	0.058626165252235296
2112	10	0.0486749603496861
2112	11	0.02931153823566035
2112	12	-0.004917562913320239
2112	13	0.0420154157992485
2112	14	0.07950601486552245
2112	15	0.049587115011860305
2112	16	-0.20903225657999513
2112	17	0.021892157404472812
2112	18	0.07975389392025534
2112	19	-0.02834754078897106
2112	20	0.07175629693681174
2112	21	0.12331974746170715
2112	22	0.14119323699317476
2112	23	0.16025692901798294
2112	24	-0.2874443418218746
2112	25	-0.011632901190779421
2112	26	0.0849121737069467
2112	27	0.09994824478233966
2112	28	0.4346557225430274
2112	29	0.2518496641746779
2112	30	0.2457597067638133
2112	31	0.1871858320626245
2112	32	0.1895685998880552
2112	33	0.12635765768617802
2112	34	0.08441206388989286
2112	35	0.1514358693067379
2112	36	0.7244807660571055
2112	37	0.5399489712448258
2112	38	0.3014377992091042
2112	39	0.14611815538472683
2112	40	0.11239019814850337
2112	41	0.0822167003472245
2112	42	0.2762425264415995
2112	43	-0.04133468207349722
2112	44	0.18830385649552284
2112	45	0.20422841665860147
2112	46	0.09687117728652339
2112	47	0.13188927418034524
2112	48	0.14868812949671906
2112	49	0.1614038972363403
2112	50	0.45021969887621793
2112	51	0.2083084900442529
1113	1	-0.026140480543816125
1113	2	-0.05039926372658243
1113	3	0.0676580846598398
1113	4	0.1001431841068694
1113	5	0.02613827723635609
1113	6	0.11496734601468717
1113	7	0.11767171938753052
1113	8	0.0643450555395404
1113	9	0.12704934378627541
1113	10	0.09520936672405611
1113	11	0.08665587098217742
1113	12	0.11009200362579463
1113	13	0.059410071436801104
1113	14	0.050466556135312146
1113	15	0.03185420330545696
1113	16	0.08086445850635471
1113	17	0.06983874531938739
1113	18	0.0878131326204894
1113	19	0.08347671157041958
1113	20	-0.011677724731768535
1113	21	0.1165174129107669
1113	22	-0.04747663651457401
1113	23	0.012320628960317492
1113	24	0.09137175025586686
1113	25	0.0602909022492355
1113	26	0.09824642151647822
1113	27	0.01611539134041351
1113	28	0.3750354621225611
1113	29	0.13339804593144322
1113	30	0.07766471337916414
1113	31	0.0888704624294192
1113	32	0.04207543697437188
1113	33	0.10357935717177469
1113	34	-0.007413862227942047
1113	35	0.020530603784486345
1113	36	0.7957432430043383
1113	37	0.51377067158149
1113	38	0.31940300122031573
1113	39	0.047302911033099804
1113	40	0.06841640857164322
1113	41	-0.03355932407262685
1113	42	0.07266777556336024
1113	43	0.0018933421497777658
1113	44	0.20152237634069792
1113	45	-0.09223375360535613
1113	46	0.030676951052292623
1113	47	0.087252405920907
1113	48	0.07659252801877159
1113	49	0.03490839281301561
1113	50	0.35641363141007076
1113	51	0.18352576783717467
2113	1	-0.3013745251719513
2113	2	0.005823710655235459
2113	3	0.06253270107482223
2113	4	0.14079730095252074
2113	5	0.11975716557576987
2113	6	0.1205085279350584
2113	7	0.1062774567604734
2113	8	0.0844991754782427
2113	9	0.1522316160360262
2113	10	0.07115963685742344
2113	11	0.025840156268635894
2113	12	0.04137052573953781
2113	13	0.07953810959570973
2113	14	0.05098259693023266
2113	15	0.027745599756876516
2113	16	0.09402598687135821
2113	17	0.1022619728227383
2113	18	0.07758083284250006
2113	19	0.043529886742817325
2113	20	0.07009807057602302
2113	21	0.09210461229710631
2113	22	0.08466065571816728
2113	23	0.014687702855724183
2113	24	0.12477510502593248
2113	25	0.07937950825043316
2113	26	0.09750868104465127
2113	27	-0.017363443208857632
2113	28	0.3890355101559777
2113	29	0.14018624987113526
2113	30	0.18367943380104634
2113	31	0.06281383649290717
2113	32	0.014515983798396803
2113	33	0.07760391498235464
2113	34	0.05234664571813141
2113	35	-0.027911357841915674
2113	36	0.7763996050481907
2113	37	0.5437559304179089
2113	38	0.22519113674877644
2113	39	0.11552070217290122
2113	40	0.08590853127373066
2113	41	0.09786527238301801
2113	42	0.25153142689608643
2113	43	0.13130160870029073
2113	44	0.4301347619747773
2113	45	0.14980943862254748
2113	46	0.04507889684221311
2113	47	0.07982525750821168
2113	48	0.09272714275257954
2113	49	0.016577677664628254
2113	50	0.3664824629281398
2113	51	0.122454584501277
1112	1	0.12185645641230991
1112	2	0.07803396920527206
1112	3	0.12960876017385203
1112	4	-0.007146270322870407
1112	5	0.03628831475460004
1112	6	-0.05389670794802015
1112	7	-0.05716039288793695
1112	8	-0.05649487369472439
1112	9	0.08050491679710348
1112	10	0.06076216501225673
1112	11	0.09666957099111073
1112	12	0.03340703463137373
1112	13	0.07955769809878888
1112	14	0.031842890852445294
1112	15	0.10052657371327456
1112	16	0.11382979053630038
1112	17	0.08624092574078901
1112	18	0.09204382932275479
1112	19	0.011209748705766742
1112	20	-0.08390979905047402
1112	21	0.04570771354308789
1112	22	-0.09078822320412883
1112	23	0.07444538979576265
1112	24	0.10244538573500961
1112	25	0.04570619787715202
1112	26	-0.23230377360231813
1112	27	0.03675079781493196
1112	28	0.3134743341321595
1112	29	0.11503886276990727
1112	30	0.17210138144439924
1112	31	0.08982762927494292
1112	32	0.1814012643490912
1112	33	0.0915874573628841
1112	34	0.06838044535250276
1112	35	0.13210835263418375
1112	36	0.770569658741806
1112	37	0.4705074174874895
1112	38	0.3244304984257269
1112	39	0.11795889735085296
1112	40	0.05469995886416967
1112	41	0.09500779949652838
1112	42	0.13447496540260317
1112	43	0.17517303784907767
1112	44	0.482588049038462
1112	45	0.1040352394482511
1112	46	-0.05570890323416933
1112	47	0.03316644444770134
1112	48	0.07976276688489037
1112	49	-0.014567386851823017
1112	50	0.3781432020700848
1112	51	0.14905670923489112
2114	1	0.1625252205841221
2114	2	0.16039514402537236
2114	3	-0.05149853708481089
2114	4	0.07083844808784079
2114	5	0.11277654612212018
2114	6	0.0018121357860962917
2114	7	0.09079157598530685
2114	8	0.016858397699145655
2114	9	0.04723789357475283
2114	10	0.06932522662641105
2114	11	0.009573140906006472
2114	12	0.04575028288041949
2114	13	0.023196368767038678
2114	14	0.02845935324145188
2114	15	0.06425732535402773
2114	16	0.11749972866368807
2114	17	0.08591705710537667
2114	18	0.10411833319015074
2114	19	-0.04297046126687576
2114	20	0.07953099355770377
2114	21	0.03615776405136728
2114	22	0.008183826807105277
2114	23	0.11918911891386585
2114	24	0.17616839896753334
2114	25	0.01402937292937878
2114	26	0.04715531665592465
2114	27	0.04115175995693221
2114	28	0.46788975820400225
2114	29	0.0652327896217102
2114	30	0.21629607845298437
2114	31	0.10806453350558343
2114	32	0.11429591777949355
2114	33	0.030054927244506757
2114	34	0.10090195397670954
2114	35	0.13645312624228723
2114	36	0.7976944756344508
2114	37	0.4755238883143349
2114	38	0.28534505564139323
2114	39	0.12736503257659848
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2108	5	-0.246618967237211
2108	6	-0.040638795379564385
2108	7	-0.02689152188814603
2108	8	0.0536735190593447
2108	9	-0.021966225483957658
2108	10	0.02006098187173677
2108	11	-0.08592584672577885
2108	12	0.03686879620688899
2108	13	-0.3572185858443859
2108	14	-0.10714193451075715
2108	15	-0.13258625818366454
2108	16	-0.039893376986391615
2108	17	0.029206506343385286
2108	18	-0.27743564807301624
2108	19	-0.13864741925829804
2108	20	-0.0997462533065061
2108	21	-0.2270761484064039
2108	22	-0.14176289108537787
2108	23	-0.039137901935340835
2108	24	-0.04966506012498684
2108	25	-0.2587029683152551
2108	26	0.013421744851569883
2108	27	-0.12643034634557182
2108	28	0.1854030552958008
2108	29	0.02349055163367808
2108	30	0.008980147792257753
2108	31	-0.06512586000810927
2108	32	-0.08485795592210366
2108	33	-0.2714113566782501
2108	34	-0.16827444083703114
2108	35	-0.32990061125244097
2108	36	-0.04042699885065559
2108	37	0.19183504753820557
2108	38	-0.11885031967986492
2108	39	-0.24814465998185398
2108	40	-0.14942052712899567
2108	41	-0.16742236740299177
2108	42	0.024696966112522034
2108	43	0.1388373282578641
2108	44	0.2619303673548359
2108	45	-0.014824654512622715
2108	46	-0.13185974674171774
2108	47	-0.3776807033258933
2108	48	-0.2537918864695001
2108	49	-0.2026596555174578
2108	50	0.2499035090536168
2108	51	-0.2136877941751223
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	2.0
19	1.0
20	13.0
21	20.0
22	103.0
23	294.0
24	701.0
25	1547.0
26	3018.0
27	5383.0
28	8434.0
29	12431.0
30	17080.0
31	23718.0
32	32476.0
33	46060.0
34	68298.0
35	118574.0
36	314459.0
37	2238444.0
38	26101.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	20.371402577440097	47.110578172317034	27.326973718941517	5.191045531301356
2	22.241750361139164	50.879794649449906	19.733178662245926	7.145276327165001
3	15.573410833420748	61.005266084319054	13.923483061253453	9.497840021006747
4	14.323118596935785	60.94435063167645	12.386781929535527	12.345748841852242
5	9.5361992734024	57.13355258782692	22.548213021029373	10.782035117741309
6	16.009657344579896	48.78491326146887	26.28520635495232	8.920223038998916
7	20.534472250046107	43.776579808155745	29.39329991724823	6.295648024549921
8	23.48676348692803	32.94932945010178	34.411060353947235	9.15284670902296
9	22.899993761050997	30.577157630817393	35.45978654567219	11.063062062459421
10	36.61502050968785	29.28192439353645	27.104119831699208	6.998935265076489
11	34.44825408839699	33.43593319251134	21.41135310463129	10.704459614460376
12	37.49529507829195	29.265675702173144	21.56976070545373	11.669268514081171
13	33.043393604323114	26.96792563172787	32.71626014086313	7.272420623085894
14	32.680231924359255	17.501588875199765	38.87783932169598	10.940339878744998
15	27.271303097055423	16.256267229954634	39.03940067696025	17.433028996029698
16	23.614559101701037	22.534295365557846	34.733566025563235	19.117579507177876
17	16.91547046817485	27.409759773039376	35.90984787248411	19.764921886301668
18	16.87639133704791	35.111536639427825	25.870453365912986	22.14161865761128
19	23.927877749508255	41.74820150296967	14.061836897418653	20.262083850103423
20	20.862599831754057	46.61355332827362	15.006934831778052	17.51691200819428
21	26.007504564373956	43.091735175125926	14.227786084949804	16.672974175550312
22	38.96384769011483	28.97614733243794	11.137963730452721	20.922041246994503
23	37.84961253384287	28.610140417488527	10.640081887919681	22.90016516074892
24	34.185326951779786	28.012915310038057	12.550502920993653	25.251254817188514
25	45.18000053476705	25.97860657530377	10.983943961897163	17.85744892803201
26	45.05655847232135	20.245766598860946	16.526735953280554	18.170938975537148
27	40.91067401902811	23.150100200263406	17.50817062360009	18.431055157108393
28	34.626886853574604	23.98913600154671	21.803104254209064	19.580872890669617
29	32.25118420051297	36.44437497043355	20.299620383949033	11.004820445104448
30	34.34222623526048	35.34049235591627	23.71547924383938	6.601802164983864
31	31.79375268669026	41.945928194496155	15.032576226587658	11.227742892225926
32	34.28892092920575	32.97606780297811	20.684926904884822	12.050084362931319
33	30.934011801897597	28.15288030336375	18.78050486123823	22.132603033500413
34	40.091349183006194	26.02231349827469	13.595663999001767	20.290673319717342
35	47.022855806918926	30.030735393831943	4.339634671827854	18.60677412742128
36	47.040304296167704	41.67028320029289	1.7275718353273974	9.561840668212005
37	34.00107227651022	53.323851502044114	1.2652382901440373	11.40983793130163
38	36.956997186988154	50.41249051302672	1.4728376042710063	11.157674695714116
39	32.20692879850869	45.31245822132363	3.134489115776382	19.346123864391302
40	36.710798660888436	38.50199406408566	2.3599681607921132	22.427239114233785
41	26.826315201302087	31.414890794396467	5.535181844795517	36.22361215950593
42	21.4713429989051	35.480971548335745	5.290320236339616	37.757365216419544
43	11.358829381199097	33.80468250262756	8.36252270188999	46.473965414283356
44	15.169798824746552	36.88271255790739	9.488241637922936	38.45924697942312
45	15.610707407689265	27.156259619808047	15.980896475268052	41.25213649723464
46	25.145706883206188	21.253219743325523	18.75788010111211	34.843193272356174
47	24.15649066660085	13.365371364869508	31.395145549195487	31.08299241933416
48	23.72535186643987	12.716520668403975	32.45580115989603	31.10232630526012
49	15.681461202992775	18.910288712507175	36.229474029174966	29.178776055325077
50	22.884053589143953	23.653466833130054	27.459397125558503	26.003082452167487
51	20.095277664082644	34.569433674830094	21.550495379406943	23.78479328168032
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2.0
1	1.5
2	1.0
3	0.5
4	0.0
5	1.0
6	2.0
7	3.0
8	4.0
9	8.0
10	12.0
11	11.0
12	10.0
13	15.5
14	21.0
15	42.5
16	64.0
17	99.5
18	135.0
19	157.0
20	179.0
21	219.0
22	259.0
23	301.0
24	343.0
25	370.0
26	479.0
27	561.0
28	742.0
29	923.0
30	1266.5
31	1610.0
32	2266.0
33	2922.0
34	4590.0
35	6258.0
36	10935.0
37	15612.0
38	28310.0
39	41008.0
40	76739.5
41	112471.0
42	515128.5
43	917786.0
44	820299.5
45	722813.0
46	460454.5
47	198096.0
48	192534.5
49	186973.0
50	181604.5
51	176236.0
52	168332.0
53	160428.0
54	162257.0
55	164086.0
56	141995.5
57	119905.0
58	82794.5
59	45684.0
60	37970.0
61	30256.0
62	18428.5
63	6601.0
64	4727.0
65	2853.0
66	2318.5
67	1784.0
68	1126.5
69	469.0
70	308.5
71	148.0
72	133.5
73	119.0
74	100.0
75	51.0
76	21.0
77	16.0
78	11.0
79	7.5
80	4.0
81	2.0
82	0.0
83	1.0
84	2.0
85	1.0
86	0.0
87	0.0
88	0.0
89	0.5
90	1.0
91	0.5
92	0.0
93	1.0
94	2.0
95	1.5
96	1.0
97	1.5
98	2.0
99	2.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2917158.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	8.700166301621046
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.39883858548625	6.124816031347069
2	13.231003863409402	2.302238678981047
3	4.627724974090879	1.2078593061826677
4	2.8773932475315127	1.0013519907474244
5	1.827802199974727	0.7951091553124465
6	1.2669673901641243	0.6613696195895207
7	0.8206686138309081	0.49979673931948054
8	0.5964670071822967	0.4151489724732941
9	0.5371884765774806	0.42062661733846884
>10	2.9247754354600133	4.966567462321788
>50	0.3998169880032244	2.465146836470016
>100	0.35725181200245454	6.51936038888061
>500	0.06706569203941057	4.201040497976568
>1k	0.04997929484371077	9.153614517486924
>5k	0.00951986199264824	5.382515047853195
>10k+	0.007536557410846523	53.883438137719494
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAAAAAATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGTCTG	303949	10.419353356931643	Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAAAAATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGTCTGA	273003	9.358526346533166	Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TGAAAAAATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGTCT	263715	9.040134267667367	Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAAATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGTCTGAAC	183945	6.305623486969167	Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AAAAATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGTCTGAA	178980	6.135423586929471	Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTGAAAAAATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGTC	57158	1.9593727868014006	No Hit
AAATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGTCTGAACT	53068	1.8191678338986095	Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGTCTGAACTCCAGT	37101	1.271820038544364	Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGTCTGAACTC	31488	1.0794067376535657	Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGTCTGAACTCCA	28405	0.9737216839129043	Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGTCTGAACTCC	25537	0.8754068171830254	Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATTCCAGGGGGCATGCCTGTTTGAGCGTCAGATCGGAAGAGCACACGTCTG	22043	0.755632708272915	Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTCCAGGGGGCATGCCTGTTTGAGCGTCATTAGATCGGAAGAGCACACGTC	18340	0.6286940919895323	No Hit
TTCCAGGGGGCATGCCTGTTTGAGCGTCAGATCGGAAGAGCACACGTCTGA	17221	0.5903348395938787	Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATTCCAGGGGGCATGCCTGTTTGAGCGTAGATCGGAAGAGCACACGTCTGA	15674	0.5373037730558303	Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TAGCCTGTGGGAATACTGCCAGCTGGGACTAGATCGGAAGAGCACACGTCT	14515	0.49757332307677543	Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTGCTCGAATATATTAGCATGGAATAGATCGGAAGAGCACACGTCTGAACT	14063	0.4820787903843399	Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GCCAAGGACGTTTTCATTAATCAAGAACAGATCGGAAGAGCACACGTCTGA	12756	0.43727490934669977	Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AATACTGCCAGCTGGGACTGAGGAAGATCGGAAGAGCACACGTCTGAACTC	10418	0.35712841059688916	Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ACTGCCAGCTGGGACTGAGGACTGCGACGAGATCGGAAGAGCACACGTCTG	9293	0.31856347856372536	Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGGGGGCATGCCTGTTTGAGCGTCATTAGATCGGAAGAGCACACGTCTGAA	8650	0.29652147741054824	Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGTCTGAACTCCAG	7631	0.26159021897339807	Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AGATTAAGCCTTTGTTGTCTGATTTGTAGATCGGAAGAGCACACGTCTGAA	7459	0.2556940693647721	Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTTGAAAAAATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGT	7369	0.2526088748021191	No Hit
TTCCAGGGGGCATGCCTGTTTGAGCGTAGATCGGAAGAGCACACGTCTGAA	7341	0.2516490364937381	Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTGCTCGAATATATTAGCATGGAATAATAGATCGGAAGAGCACACGTCTGA	7267	0.24911232096444555	Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TAGCCTGTGGGAATACTGCCAGCTGGGACTGAGGAAGATCGGAAGAGCACA	7143	0.24486160845590124	No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATTAGATCGGAAGAGCACACGT	7001	0.23999385703482637	No Hit
CTATGTTCCTTGGAACAGGACGTCAGATCGGAAGAGCACACGTCTGAACTC	6643	0.22772163866338402	Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AAGCCTTTGTTGTCTGATTTGTAGATCGGAAGAGCACACGTCTGAACTCCA	6578	0.22549344259035678	Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ATAGATGGATACGAATAAGGCGTCAGATCGGAAGAGCACACGTCTGAACTC	6465	0.22161980941724788	Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GAGATTAAGCCTTTGTTGTCTGATTTGTAGATCGGAAGAGCACACGTCTGA	6450	0.22110561032347237	Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTACGATCTGCTGAGATTAAGCCTTTGTTAGATCGGAAGAGCACACGTCTG	6261	0.21462670174190085	Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACAC	6008	0.20595387702688714	No Hit
AGAGGTGCACAATCGACCGATCCTGAAGATCGGAAGAGCACACGTCTGAAC	5665	0.1941958577492203	Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCCAAGGACGTTTTCATTAATCAGATCGGAAGAGCACACGTCTGAACTCCA	5659	0.1939901781117101	Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TAAGCCTTTGTTGTCTGATTTGTAGATCGGAAGAGCACACGTCTGAACTCC	5471	0.18754554946972363	Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGGGCCAGCATCAGTTTTGGTGGCAGGATAGATCGGAAGAGCACACGTCTG	5433	0.1862429117654923	Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTACGATCTGCTGAGATTAAGCCTTTAGATCGGAAGAGCACACGTCTGAAC	5433	0.1862429117654923	Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGTTTTGGTGGCAGGATAAATCCATAGGAGATCGGAAGAGCACACGTCTGA	5424	0.185934392309227	Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCCAAGGACGTTTTCATTAATCAAGAACGAAGATCGGAAGAGCACACGTCT	5150	0.17654168886292756	Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGTTTTGGTGGCAGGATAAATCCAAGATCGGAAGAGCACACGTCTGAACTC	5116	0.17537617091703636	Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ATACTGCCAGCTGGGACTGAGGAAGATCGGAAGAGCACACGTCTGAACTCC	5059	0.17342221436068941	Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CTGCCAGCTGGGACTGAGGACTGCGACGAGATCGGAAGAGCACACGTCTGA	4751	0.1628639929684988	Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AATATAGATGGATACGAATAAGGCGTCAGATCGGAAGAGCACACGTCTGAA	4452	0.15261429103257348	Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGCCTGTGGGAATACTGCCAGCTGGGACTAGATCGGAAGAGCACACGTCTG	4420	0.15151733296585237	Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCCAGGGGGCATGCCTGTTTGAGCGTCATTAGATCGGAAGAGCACACGTCT	4394	0.1506260545366415	Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGGGAATACTGCCAGCTGGGACTGAGGAAGATCGGAAGAGCACACGTCTGA	4370	0.14980333598660064	Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AATTCTAGAGCTAATACATGCTAGATCGGAAGAGCACACGTCTGAACTCCA	4342	0.1488434976782197	Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ATTAAGCCTTTGTTGTCTGATTTGTAGATCGGAAGAGCACACGTCTGAACT	4182	0.14335870734461417	Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GTGAATCATCGAATCTTTGAACGCACAGATCGGAAGAGCACACGTCTGAAC	4130	0.14157615048619238	Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AAAAAAATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGTCTG	4075	0.1396907538090155	Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAGAGCATAGCTGTTGGGACCCGAAAAGATCGGAAGAGCACACGTCTGAAC	3919	0.1343430832337501	Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGGGGGCATGCCTGTTTGAGCGTCATTTAGATCGGAAGAGCACACGTCTGA	3908	0.13396600389831473	Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTGGGAATACTGCCAGCTGGGACTGAGGAAGATCGGAAGAGCACACGTCTG	3888	0.13328040510661404	Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGAGATTAAGCCTTTGTTGTCTGATTTGTAGATCGGAAGAGCACACGTCTG	3866	0.13252624643574326	Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACGATCTGCTGAGATTAAGCCTTTGTTAGATCGGAAGAGCACACGTCTGAA	3747	0.1284469336251242	Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGATCAACTTAGAACTGGTACGGACAAGAGATCGGAAGAGCACACGTCTGA	3673	0.12591021809583164	Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATGATTAATAGGGACGGTCGGGGGCATCAGATCGGAAGAGCACACGTCTGA	3514	0.12045970770181115	Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATTATAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGTCTGAACTCC	3509	0.12028830800388597	Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTAAGCCTTTGTTGTCTGATTTGTAGATCGGAAGAGCACACGTCTGAACTC	3479	0.11925990981633493	Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TAGCCTGTGGGAATACTGCCAGCTGGGACTGAGATCGGAAGAGCACACGTC	3462	0.11867715084338937	No Hit
AGATGGATACGAATAAGGCGTCAGATCGGAAGAGCACACGTCTGAACTCCA	3393	0.11631183501202198	Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AGTTTTGGTGGCAGGATAAATCCAGATCGGAAGAGCACACGTCTGAACTCC	3362	0.11524915688488592	Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGGAATACTGCCAGCTGGGACTGAGGAAGATCGGAAGAGCACACGTCTGAA	3358	0.11511203712654576	Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CTATGTTCCTTGGAACAGGACGTCATAGATCGGAAGAGCACACGTCTGAAC	3306	0.11332948026812398	Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GATTAAGCCTTTGTTGTCTGATTTGTAGATCGGAAGAGCACACGTCTGAAC	3230	0.11072420485966135	Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TTACGATCTGCTGAGATTAAGCCTTTGTAGATCGGAAGAGCACACGTCTGA	3172	0.10873596836372934	Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TAGCCTGTGGGAATACTGCCAGCTGGGACTGAGGAGATCGGAAGAGCACAC	3114	0.10674773186779736	No Hit
ACTGCCAGCTGGGACTGAGGACTGCGACGTAGATCGGAAGAGCACACGTCT	3084	0.10571933368024632	Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGGGCCAGCATCAGTTTTGGTGGCAGATCGGAAGAGCACACGTCTGAACTC	3073	0.10534225434481095	Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ACGATCTGCTGAGATTAAGCCTTTAGATCGGAAGAGCACACGTCTGAACTC	3020	0.10352541754680411	Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ACGATCAACTTAGAACTGGTACGGACAAGAGATCGGAAGAGCACACGTCTG	2930	0.100440222984151	Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA Adapter	Nextera Transposase Sequence
1	0.0052105508169252406	0.0	0.0
2	0.005416230454435447	0.0	0.0
3	0.005450510394020482	0.0	0.0
4	0.005519070273190551	0.0	0.0
5	0.005724749910700757	0.0	0.0
6	0.0058275897294558605	0.0	0.0
7	0.005861869669040895	0.0	0.0
8	0.005998989427381033	0.0	0.0
9	0.006101829246136136	0.0	0.0
10	0.006273228944061309	0.0	0.0
11	0.006410348702401447	0.0	0.0
12	0.006718868158666757	0.0	0.0
13	0.006958827735761998	0.0	0.0
14	0.007712986406632757	0.0	0.0
15	0.009632663023394687	0.0	0.0
16	0.013540576136088618	0.0	0.0
17	0.026361273540891513	0.0	0.0
18	0.08360877264789908	0.0	0.0
19	0.35657993156352863	0.0	0.0
20	0.7891584891870786	0.0	0.0
21	2.7854850508611464	0.0	0.0
22	4.059533285478538	0.0	0.0
23	6.989096922415584	0.0	0.0
24	10.319084533645418	0.0	0.0
25	14.929530728195045	0.0	0.0
26	19.965151013417852	0.0	0.0
27	29.838322092941144	0.0	0.0
28	40.28314544498447	0.0	0.0
29	59.0052372891698	0.0	0.0
30	76.49342270799183	0.0	0.0
31	88.93426410225294	0.0	0.0
32	92.58617462612584	0.0	0.0
33	93.60644846799522	0.0	0.0
34	93.98750427642247	0.0	0.0
35	94.40547957978279	0.0	0.0
36	94.73765219436177	0.0	0.0
37	94.82294068404934	0.0	0.0
38	94.86328817294093	0.0	0.0
39	94.91090300902454	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGTTTA	30	2.1666547E-6	45.000004	8
CGCCTTA	25	3.892287E-5	45.0	23
CCGTACA	45	3.8562575E-10	45.0	1
CGTAATA	20	7.0350745E-4	45.0	19
ACGTACA	45	3.8562575E-10	45.0	1
TTGCTCG	2710	0.0	43.837635	1
TAGCCTG	3700	0.0	43.722973	1
ATTCCAG	5305	0.0	43.685204	1
GGTACAC	555	0.0	42.972973	2
GTTGCAC	105	0.0	42.85714	4
TGTACAC	460	0.0	42.554344	2
TCGTACA	90	0.0	42.5	1
TGCATGC	255	0.0	42.35294	5
ATGGGGC	155	0.0	42.096775	4
TTTTCCT	225	0.0	42.0	4
TGGGCCA	1140	0.0	41.842106	1
TATCTGC	270	0.0	41.666664	5
TACACGG	1510	0.0	41.42384	4
GTAATAC	125	0.0	41.4	4
GGGTACA	120	0.0	41.250004	1
>>END_MODULE