FastQCFastQC Report
Wed 16 Sep 2015
000000000-AHBE6 l01n01 150915_gms8.341000000347fb.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AHBE6 l01n01 150915_gms8.341000000347fb.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2917158
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAAAAAATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGTCTG30394910.419353356931643Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAAAAATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGTCTGA2730039.358526346533166Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TGAAAAAATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGTCT2637159.040134267667367Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAAATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGTCTGAAC1839456.305623486969167Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AAAAATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGTCTGAA1789806.135423586929471Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTGAAAAAATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGTC571581.9593727868014006No Hit
AAATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGTCTGAACT530681.8191678338986095Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGTCTGAACTCCAGT371011.271820038544364Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGTCTGAACTC314881.0794067376535657Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGTCTGAACTCCA284050.9737216839129043Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGTCTGAACTCC255370.8754068171830254Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATTCCAGGGGGCATGCCTGTTTGAGCGTCAGATCGGAAGAGCACACGTCTG220430.755632708272915Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTCCAGGGGGCATGCCTGTTTGAGCGTCATTAGATCGGAAGAGCACACGTC183400.6286940919895323No Hit
TTCCAGGGGGCATGCCTGTTTGAGCGTCAGATCGGAAGAGCACACGTCTGA172210.5903348395938787Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATTCCAGGGGGCATGCCTGTTTGAGCGTAGATCGGAAGAGCACACGTCTGA156740.5373037730558303Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TAGCCTGTGGGAATACTGCCAGCTGGGACTAGATCGGAAGAGCACACGTCT145150.49757332307677543Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTGCTCGAATATATTAGCATGGAATAGATCGGAAGAGCACACGTCTGAACT140630.4820787903843399Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GCCAAGGACGTTTTCATTAATCAAGAACAGATCGGAAGAGCACACGTCTGA127560.43727490934669977Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AATACTGCCAGCTGGGACTGAGGAAGATCGGAAGAGCACACGTCTGAACTC104180.35712841059688916Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ACTGCCAGCTGGGACTGAGGACTGCGACGAGATCGGAAGAGCACACGTCTG92930.31856347856372536Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGGGGGCATGCCTGTTTGAGCGTCATTAGATCGGAAGAGCACACGTCTGAA86500.29652147741054824Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGTCTGAACTCCAG76310.26159021897339807Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AGATTAAGCCTTTGTTGTCTGATTTGTAGATCGGAAGAGCACACGTCTGAA74590.2556940693647721Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTTGAAAAAATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGT73690.2526088748021191No Hit
TTCCAGGGGGCATGCCTGTTTGAGCGTAGATCGGAAGAGCACACGTCTGAA73410.2516490364937381Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTGCTCGAATATATTAGCATGGAATAATAGATCGGAAGAGCACACGTCTGA72670.24911232096444555Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TAGCCTGTGGGAATACTGCCAGCTGGGACTGAGGAAGATCGGAAGAGCACA71430.24486160845590124No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATTAGATCGGAAGAGCACACGT70010.23999385703482637No Hit
CTATGTTCCTTGGAACAGGACGTCAGATCGGAAGAGCACACGTCTGAACTC66430.22772163866338402Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AAGCCTTTGTTGTCTGATTTGTAGATCGGAAGAGCACACGTCTGAACTCCA65780.22549344259035678Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ATAGATGGATACGAATAAGGCGTCAGATCGGAAGAGCACACGTCTGAACTC64650.22161980941724788Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GAGATTAAGCCTTTGTTGTCTGATTTGTAGATCGGAAGAGCACACGTCTGA64500.22110561032347237Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTACGATCTGCTGAGATTAAGCCTTTGTTAGATCGGAAGAGCACACGTCTG62610.21462670174190085Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACAC60080.20595387702688714No Hit
AGAGGTGCACAATCGACCGATCCTGAAGATCGGAAGAGCACACGTCTGAAC56650.1941958577492203Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCCAAGGACGTTTTCATTAATCAGATCGGAAGAGCACACGTCTGAACTCCA56590.1939901781117101Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TAAGCCTTTGTTGTCTGATTTGTAGATCGGAAGAGCACACGTCTGAACTCC54710.18754554946972363Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGGGCCAGCATCAGTTTTGGTGGCAGGATAGATCGGAAGAGCACACGTCTG54330.1862429117654923Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTACGATCTGCTGAGATTAAGCCTTTAGATCGGAAGAGCACACGTCTGAAC54330.1862429117654923Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGTTTTGGTGGCAGGATAAATCCATAGGAGATCGGAAGAGCACACGTCTGA54240.185934392309227Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCCAAGGACGTTTTCATTAATCAAGAACGAAGATCGGAAGAGCACACGTCT51500.17654168886292756Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGTTTTGGTGGCAGGATAAATCCAAGATCGGAAGAGCACACGTCTGAACTC51160.17537617091703636Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ATACTGCCAGCTGGGACTGAGGAAGATCGGAAGAGCACACGTCTGAACTCC50590.17342221436068941Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CTGCCAGCTGGGACTGAGGACTGCGACGAGATCGGAAGAGCACACGTCTGA47510.1628639929684988Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AATATAGATGGATACGAATAAGGCGTCAGATCGGAAGAGCACACGTCTGAA44520.15261429103257348Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGCCTGTGGGAATACTGCCAGCTGGGACTAGATCGGAAGAGCACACGTCTG44200.15151733296585237Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCCAGGGGGCATGCCTGTTTGAGCGTCATTAGATCGGAAGAGCACACGTCT43940.1506260545366415Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGGGAATACTGCCAGCTGGGACTGAGGAAGATCGGAAGAGCACACGTCTGA43700.14980333598660064Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AATTCTAGAGCTAATACATGCTAGATCGGAAGAGCACACGTCTGAACTCCA43420.1488434976782197Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ATTAAGCCTTTGTTGTCTGATTTGTAGATCGGAAGAGCACACGTCTGAACT41820.14335870734461417Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GTGAATCATCGAATCTTTGAACGCACAGATCGGAAGAGCACACGTCTGAAC41300.14157615048619238Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AAAAAAATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGTCTG40750.1396907538090155Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAGAGCATAGCTGTTGGGACCCGAAAAGATCGGAAGAGCACACGTCTGAAC39190.1343430832337501Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGGGGGCATGCCTGTTTGAGCGTCATTTAGATCGGAAGAGCACACGTCTGA39080.13396600389831473Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTGGGAATACTGCCAGCTGGGACTGAGGAAGATCGGAAGAGCACACGTCTG38880.13328040510661404Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGAGATTAAGCCTTTGTTGTCTGATTTGTAGATCGGAAGAGCACACGTCTG38660.13252624643574326Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACGATCTGCTGAGATTAAGCCTTTGTTAGATCGGAAGAGCACACGTCTGAA37470.1284469336251242Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGATCAACTTAGAACTGGTACGGACAAGAGATCGGAAGAGCACACGTCTGA36730.12591021809583164Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATGATTAATAGGGACGGTCGGGGGCATCAGATCGGAAGAGCACACGTCTGA35140.12045970770181115Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATTATAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGTCTGAACTCC35090.12028830800388597Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTAAGCCTTTGTTGTCTGATTTGTAGATCGGAAGAGCACACGTCTGAACTC34790.11925990981633493Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TAGCCTGTGGGAATACTGCCAGCTGGGACTGAGATCGGAAGAGCACACGTC34620.11867715084338937No Hit
AGATGGATACGAATAAGGCGTCAGATCGGAAGAGCACACGTCTGAACTCCA33930.11631183501202198Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AGTTTTGGTGGCAGGATAAATCCAGATCGGAAGAGCACACGTCTGAACTCC33620.11524915688488592Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGGAATACTGCCAGCTGGGACTGAGGAAGATCGGAAGAGCACACGTCTGAA33580.11511203712654576Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CTATGTTCCTTGGAACAGGACGTCATAGATCGGAAGAGCACACGTCTGAAC33060.11332948026812398Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GATTAAGCCTTTGTTGTCTGATTTGTAGATCGGAAGAGCACACGTCTGAAC32300.11072420485966135Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TTACGATCTGCTGAGATTAAGCCTTTGTAGATCGGAAGAGCACACGTCTGA31720.10873596836372934Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TAGCCTGTGGGAATACTGCCAGCTGGGACTGAGGAGATCGGAAGAGCACAC31140.10674773186779736No Hit
ACTGCCAGCTGGGACTGAGGACTGCGACGTAGATCGGAAGAGCACACGTCT30840.10571933368024632Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGGGCCAGCATCAGTTTTGGTGGCAGATCGGAAGAGCACACGTCTGAACTC30730.10534225434481095Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ACGATCTGCTGAGATTAAGCCTTTAGATCGGAAGAGCACACGTCTGAACTC30200.10352541754680411Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ACGATCAACTTAGAACTGGTACGGACAAGAGATCGGAAGAGCACACGTCTG29300.100440222984151Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTTTA302.1666547E-645.0000048
CGCCTTA253.892287E-545.023
CCGTACA453.8562575E-1045.01
CGTAATA207.0350745E-445.019
ACGTACA453.8562575E-1045.01
TTGCTCG27100.043.8376351
TAGCCTG37000.043.7229731
ATTCCAG53050.043.6852041
GGTACAC5550.042.9729732
GTTGCAC1050.042.857144
TGTACAC4600.042.5543442
TCGTACA900.042.51
TGCATGC2550.042.352945
ATGGGGC1550.042.0967754
TTTTCCT2250.042.04
TGGGCCA11400.041.8421061
TATCTGC2700.041.6666645
TACACGG15100.041.423844
GTAATAC1250.041.44
GGGTACA1200.041.2500041
Produced by FastQC (version 0.11.2)