##FastQC	0.11.2
>>Basic Statistics	pass
#Measure	Value
Filename	000000000-AHBE6 l01n01 150915_gms7.341000000347ee.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3085630
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.93451418348927	33.0	32.0	34.0	32.0	34.0
2	33.24879522172133	33.0	33.0	34.0	32.0	35.0
3	33.4001698194534	34.0	33.0	34.0	32.0	35.0
4	33.45608222632007	34.0	33.0	34.0	32.0	35.0
5	32.831392616742775	34.0	33.0	34.0	32.0	35.0
6	35.84801029287374	37.0	35.0	37.0	33.0	37.0
7	35.73997854571028	37.0	35.0	37.0	33.0	37.0
8	35.6491737505793	37.0	35.0	37.0	33.0	37.0
9	35.91852684865003	37.0	37.0	37.0	33.0	37.0
10	35.961534921555725	37.0	37.0	37.0	33.0	37.0
11	35.78130235964779	37.0	35.0	37.0	33.0	37.0
12	35.887559428706616	37.0	35.0	37.0	33.0	37.0
13	37.48848371321254	38.0	38.0	38.0	37.0	38.0
14	37.52084955098311	38.0	38.0	38.0	37.0	38.0
15	37.46080541088854	38.0	38.0	38.0	37.0	38.0
16	37.445077666473296	38.0	38.0	38.0	37.0	38.0
17	37.46308274161192	38.0	38.0	38.0	37.0	38.0
18	37.415663251912896	38.0	38.0	38.0	37.0	38.0
19	37.461300933682914	38.0	38.0	38.0	37.0	38.0
20	37.46926818834403	38.0	38.0	38.0	37.0	38.0
21	37.49128087294977	38.0	38.0	38.0	37.0	38.0
22	37.52505420286943	38.0	38.0	38.0	37.0	38.0
23	38.16228938660824	39.0	38.0	39.0	37.0	39.0
24	38.08761646730165	39.0	38.0	39.0	37.0	39.0
25	38.12477257480644	39.0	38.0	39.0	37.0	39.0
26	38.04517715993168	39.0	38.0	39.0	37.0	39.0
27	37.88760577256508	38.0	38.0	39.0	37.0	39.0
28	37.446998181894784	38.0	38.0	39.0	36.0	39.0
29	37.701506013358696	38.0	38.0	39.0	36.0	39.0
30	37.730048644847244	38.0	38.0	39.0	37.0	39.0
31	37.85732087126454	38.0	38.0	39.0	37.0	39.0
32	37.896219248581325	39.0	38.0	39.0	37.0	39.0
33	37.97027381766446	39.0	38.0	39.0	37.0	39.0
34	38.01023324248209	39.0	38.0	39.0	37.0	39.0
35	37.82921737214118	38.0	38.0	39.0	37.0	39.0
36	37.01572061459086	38.0	38.0	39.0	35.0	39.0
37	37.32315378058938	38.0	38.0	39.0	36.0	39.0
38	37.53037337593943	38.0	38.0	39.0	36.0	39.0
39	37.89100702287701	38.0	38.0	39.0	37.0	39.0
40	38.004063999896296	39.0	38.0	39.0	37.0	39.0
41	38.062548004783466	39.0	38.0	39.0	37.0	39.0
42	37.92960983656498	39.0	38.0	39.0	37.0	39.0
43	38.00630244066852	39.0	38.0	39.0	37.0	39.0
44	37.799320722186394	39.0	38.0	39.0	37.0	39.0
45	38.02661984748657	39.0	38.0	39.0	37.0	39.0
46	37.85081231385487	39.0	38.0	39.0	37.0	39.0
47	37.960642073093666	39.0	38.0	39.0	37.0	39.0
48	38.006388322644	39.0	38.0	39.0	37.0	39.0
49	38.031825267449435	39.0	38.0	39.0	37.0	39.0
50	37.50258585766926	39.0	38.0	39.0	36.0	39.0
51	37.705689599854814	39.0	38.0	39.0	37.0	39.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	-0.06683386739149455
1101	2	0.11242824916104865
1101	3	0.15344791368652722
1101	4	0.1805171943861339
1101	5	0.03847346758854542
1101	6	0.10020208192833024
1101	7	0.13718000635297045
1101	8	0.11661925914982163
1101	9	0.09119294006666934
1101	10	0.0663055117882223
1101	11	0.11768459085873673
1101	12	0.09844800727837111
1101	13	0.045487012180977615
1101	14	0.02020150053166958
1101	15	0.010530037063091413
1101	16	0.048037681268304766
1101	17	-0.02252786968423237
1101	18	0.08117101073144539
1101	19	0.06798189021571233
1101	20	0.05952310512601855
1101	21	0.02614828035922301
1101	22	0.07644237910611906
1101	23	0.11450899479213916
1101	24	0.10042632505786742
1101	25	0.06376471347874002
1101	26	0.09997902754929555
1101	27	0.06306035416901068
1101	28	0.45027932688844174
1101	29	0.16091962362939682
1101	30	0.2316886261427058
1101	31	0.09574400417549356
1101	32	0.10211535492944535
1101	33	0.11318346472909013
1101	34	0.09623473920720471
1101	35	0.17903582175965482
1101	36	0.7973840988500882
1101	37	0.5328694233051507
1101	38	0.2422608485365103
1101	39	0.14990630895040624
1101	40	0.03664470499535355
1101	41	0.001150183528856985
1101	42	0.1804522453373849
1101	43	0.18716954498331972
1101	44	0.38745472604768594
1101	45	0.14289661357270944
1101	46	0.12145363176544066
1101	47	0.08309311027333166
1101	48	0.14079294574207069
1101	49	0.003406398536341726
1101	50	0.5115409266409401
1101	51	0.14572207194591158
1102	1	-0.04699859111955362
1102	2	0.12162344133631109
1102	3	0.07202229416476769
1102	4	0.13272278619295008
1102	5	0.1026133496463899
1102	6	0.026222868799919752
1102	7	0.1542510688959169
1102	8	0.09810210629509442
1102	9	-0.02195690932175154
1102	10	-0.054826783186875616
1102	11	0.0032778745508821316
1102	12	0.1258729587341847
1102	13	0.060650155745747725
1102	14	0.06570682008865703
1102	15	0.10260530334746676
1102	16	0.021091841387502086
1102	17	-0.006231709175317235
1102	18	0.01125363167605542
1102	19	0.03663281359830961
1102	20	0.08994655970259657
1102	21	0.16311631752738975
1102	22	0.08929393364424243
1102	23	0.12257575977681512
1102	24	0.09641481522770334
1102	25	0.11574742261346671
1102	26	0.15862914042869392
1102	27	0.18892141598624335
1102	28	0.4916919721633093
1102	29	0.2134162791827734
1102	30	0.22729655807818006
1102	31	0.1766865500969601
1102	32	0.22431811413275682
1102	33	0.1591018294972315
1102	34	0.15784267515361705
1102	35	0.19928329540195477
1102	36	0.8190583825782838
1102	37	0.577318740841406
1102	38	0.36761690702507366
1102	39	0.18334325875573398
1102	40	0.20454761594097448
1102	41	0.24839259355611176
1102	42	0.27654588570148064
1102	43	0.2594009343030237
1102	44	0.4880616023398403
1102	45	0.2319524497427352
1102	46	0.2425176918607832
1102	47	0.20807786340548518
1102	48	0.2149077644845505
1102	49	0.1904281113642199
1102	50	0.5472511414383092
1102	51	0.19747592928323598
1103	1	0.15952871356958553
1103	2	0.10668315052696897
1103	3	0.006335713974870316
1103	4	0.041228124100882724
1103	5	-0.09203871800297492
1103	6	0.1255202849240007
1103	7	-0.5985528959777042
1103	8	-0.9294774126059124
1103	9	-0.8980177992615523
1103	10	-0.24910246109714507
1103	11	-0.14734094625438843
1103	12	-0.004604244857389972
1103	13	0.07445678268306466
1103	14	0.1353098820078671
1103	15	0.124398747947005
1103	16	0.07397675098274448
1103	17	0.0631453501420225
1103	18	0.0799464842401747
1103	19	0.16679855766747664
1103	20	0.17399849963192082
1103	21	0.18073604078080763
1103	22	0.03969916143898189
1103	23	0.0647734639823625
1103	24	-0.20856940515066924
1103	25	0.08026612525193144
1103	26	0.040380238195901086
1103	27	0.19095098652022813
1103	28	0.4967478989934051
1103	29	0.13322808815623688
1103	30	0.24745911136020027
1103	31	0.14289357203923458
1103	32	0.22202201391492338
1103	33	0.06117034562966239
1103	34	0.16887853124502783
1103	35	0.19690137902537685
1103	36	0.8446385487262802
1103	37	0.6049294549854878
1103	38	0.40377952052543975
1103	39	0.17064065436147047
1103	40	0.18794871399180835
1103	41	0.21416796991097442
1103	42	0.347078497358126
1103	43	0.14931187723087902
1103	44	0.39140644168296035
1103	45	0.19005858336161907
1103	46	0.13315048636382443
1103	47	0.17153789941268371
1103	48	0.16353272470414026
1103	49	0.22109676970318048
1103	50	0.5307379386866202
1103	51	0.2714852232099858
2112	1	-0.1503335417121363
2112	2	0.09202361870965348
2112	3	0.057093271233370047
2112	4	0.13193844979426217
2112	5	0.194759936138297
2112	6	0.15647382183424696
2112	7	-0.48030138198580374
2112	8	0.014514077080605148
2112	9	0.10139335300794983
2112	10	0.04537680198086491
2112	11	0.02639335807902654
2112	12	0.024385830599520375
2112	13	0.0404166136800157
2112	14	0.06027301418483688
2112	15	0.06603533830794106
2112	16	-0.1995338913970386
2112	17	0.028242819341876668
2112	18	0.11252323008779541
2112	19	0.015633884849414414
2112	20	0.0924553619163504
2112	21	0.11257308915193676
2112	22	0.1762894503267063
2112	23	0.17663646237137698
2112	24	-0.3203973306792065
2112	25	-0.026867419105656154
2112	26	0.0923152281655959
2112	27	0.11513787950408982
2112	28	0.43522176152896463
2112	29	0.22403950471644407
2112	30	0.23509369043073747
2112	31	0.19129520440034042
2112	32	0.13618679887629526
2112	33	0.12027231105496128
2112	34	0.08432869670663479
2112	35	0.13275075246414758
2112	36	0.8082331907764271
2112	37	0.5423937395534182
2112	38	0.29819989544007797
2112	39	0.14590415066813733
2112	40	0.08866937990693913
2112	41	0.06202336747375625
2112	42	0.2557438188079644
2112	43	0.001253813966265227
2112	44	0.20766287863987998
2112	45	0.17975743505090946
2112	46	0.0753203316829314
2112	47	0.09827374516356713
2112	48	0.12508131710557535
2112	49	0.16087900758442686
2112	50	0.49378612048215587
2112	51	0.25460415878036713
1113	1	-0.0341504950568563
1113	2	-0.0335215023223725
1113	3	0.10096752784160401
1113	4	0.15082274923351235
1113	5	0.07542361778058648
1113	6	0.1424214126966561
1113	7	0.1681187018631931
1113	8	0.14425228707592908
1113	9	0.15296280357895142
1113	10	0.12910632327849214
1113	11	0.0930192393944651
1113	12	0.09766517099254202
1113	13	0.08841684240029934
1113	14	0.07118871292094298
1113	15	0.05440483057854806
1113	16	0.08523628394297589
1113	17	0.07538354559734017
1113	18	0.10889659572709576
1113	19	0.09719521224756988
1113	20	-0.00370228260874228
1113	21	0.11043615833987985
1113	22	-0.02241391021694028
1113	23	0.042945738100364395
1113	24	0.1070491497334558
1113	25	0.039275453320087195
1113	26	0.09516592199403817
1113	27	0.020411538150575836
1113	28	0.3503313801989094
1113	29	0.12958866205710962
1113	30	0.06880102830155721
1113	31	0.08443367146192315
1113	32	0.08162085691859744
1113	33	0.14578676753078668
1113	34	0.059521146287494275
1113	35	0.033844629297931306
1113	36	0.7907932111102127
1113	37	0.5171100953141021
1113	38	0.3265081562228076
1113	39	0.08495042776613104
1113	40	0.07899060696801286
1113	41	0.013836141327210782
1113	42	0.08238598947037445
1113	43	-0.06169464499886601
1113	44	0.2014386768611729
1113	45	-0.10165346924668484
1113	46	0.011244483946256878
1113	47	0.04193841705542667
1113	48	0.03989167016170825
1113	49	0.037935289219056756
1113	50	0.36009382724578387
1113	51	0.15589595768805253
2113	1	-0.37627954220389626
2113	2	-0.014282590035037401
2113	3	0.043832751351722266
2113	4	0.13914967254903843
2113	5	0.12335149926610711
2113	6	0.1842553022879727
2113	7	0.1611845863570167
2113	8	0.09952623177556319
2113	9	0.13500375219409477
2113	10	0.11017146738024763
2113	11	0.035770831271271675
2113	12	0.07404497205854454
2113	13	0.08799217985912833
2113	14	0.05424679925020115
2113	15	0.09980017864631208
2113	16	0.12370115467459897
2113	17	0.1443201540983452
2113	18	0.10983479494044701
2113	19	0.041790556908829046
2113	20	0.05760629879102197
2113	21	0.07163421033965278
2113	22	0.0810850443353246
2113	23	0.02012592841466443
2113	24	0.1620398857371299
2113	25	0.08256488011359409
2113	26	0.10659147259946167
2113	27	-0.00832437649267348
2113	28	0.4129920638324336
2113	29	0.1525230904132755
2113	30	0.24517349918912146
2113	31	0.0683235364526098
2113	32	-0.009169398721006417
2113	33	0.06633504823727066
2113	34	0.04823784323567537
2113	35	0.006496391012831282
2113	36	0.8231346582941441
2113	37	0.5350297386285661
2113	38	0.26570776028549403
2113	39	0.15187333362995759
2113	40	0.05135835485829432
2113	41	0.04008200593722222
2113	42	0.18734258581645946
2113	43	0.1004441137780887
2113	44	0.39189848469580824
2113	45	0.18287450384730874
2113	46	0.04889044828617273
2113	47	0.11227393456060497
2113	48	0.10712295580053421
2113	49	0.0061473822629167785
2113	50	0.39856726317589164
2113	51	0.09690955658844302
1112	1	0.18338875659483733
1112	2	0.08671586669464659
1112	3	0.1290014017377672
1112	4	-0.006816315314182475
1112	5	0.07988294986870415
1112	6	-0.03710319179779731
1112	7	-0.010671439253947312
1112	8	-0.10329967856022648
1112	9	0.05216628200484763
1112	10	0.027927997784999548
1112	11	0.12624994941837286
1112	12	0.044154448096875853
1112	13	0.08542557650201132
1112	14	0.026425459175897004
1112	15	0.10570005806398086
1112	16	0.13110748619160262
1112	17	0.07697386936075645
1112	18	0.1261952105103461
1112	19	0.07985942106464705
1112	20	-0.1092231328605422
1112	21	0.0628240908575961
1112	22	0.02227183532448862
1112	23	0.04356184513121519
1112	24	0.15164786348739767
1112	25	0.09361386744716782
1112	26	-0.24773329674507494
1112	27	0.02664076519183567
1112	28	0.3846862173447647
1112	29	0.06567606285478433
1112	30	0.19533411733392825
1112	31	0.10180090194578639
1112	32	0.21951890901868154
1112	33	0.079296661989396
1112	34	0.11333650912484927
1112	35	0.13393267655188623
1112	36	0.819721556066817
1112	37	0.49252433248679495
1112	38	0.33284306062238045
1112	39	0.12795675240111848
1112	40	0.07990538735789698
1112	41	0.1369204308388845
1112	42	0.2175711970727363
1112	43	0.2547256830794211
1112	44	0.4839035846060469
1112	45	0.07099866469874172
1112	46	-0.04540890688274857
1112	47	0.07585666098918153
1112	48	0.08957219121419513
1112	49	0.004984033273480293
1112	50	0.41165950156457143
1112	51	0.19068107723742855
2114	1	0.17382639008204137
2114	2	0.1414305811860075
2114	3	-0.03680629989968054
2114	4	0.07645263132172886
2114	5	0.02822714583730601
2114	6	-0.02832340705798231
2114	7	0.051131585960128234
2114	8	-0.020562331985694016
2114	9	0.04690763815392529
2114	10	0.06205749811748262
2114	11	0.04759425460378708
2114	12	0.05042630776802781
2114	13	0.02470651499925225
2114	14	0.022939598445717024
2114	15	0.06651530081495594
2114	16	0.11386950767325033
2114	17	0.048760613507411676
2114	18	0.07597104204558036
2114	19	-0.04583044223296895
2114	20	0.10699911635053638
2114	21	0.023349369197333658
2114	22	-0.0012687323236448833
2114	23	0.10177922858370891
2114	24	0.134413163430402
2114	25	0.029407053166877972
2114	26	0.07529020535366726
2114	27	0.030093841308513447
2114	28	0.45302218845307607
2114	29	0.09259254860258181
2114	30	0.17598356193321507
2114	31	0.060973488763359285
2114	32	0.0769747287344913
2114	33	0.028312860981110077
2114	34	0.0794938823159228
2114	35	0.1362873865623584
2114	36	0.8327111216866712
2114	37	0.5114005059476128
2114	38	0.337917164613998
2114	39	0.17941260291163275
2114	40	0.12353212536649494
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2108	7	-0.0417779589968319
2108	8	0.0033323257990502952
2108	9	-0.013479562050086713
2108	10	0.016859520807436468
2108	11	-0.07275369844890633
2108	12	0.04130880888759947
2108	13	-0.3516089798507238
2108	14	-0.14896922190060025
2108	15	-0.17914325518842844
2108	16	-0.06913860609994771
2108	17	-8.10201941305877E-5
2108	18	-0.30367983868288206
2108	19	-0.13719435848913264
2108	20	-0.1515387717670933
2108	21	-0.20947494848689274
2108	22	-0.2151000100019047
2108	23	-0.09345202483719817
2108	24	-0.12205507498826762
2108	25	-0.2467213024334569
2108	26	-0.04822636428359317
2108	27	-0.17414645264083362
2108	28	0.1847895231298935
2108	29	1.3821056178642266E-5
2108	30	0.02627527213871872
2108	31	-0.11366544988420202
2108	32	-0.10360998229202778
2108	33	-0.29665440714348534
2108	34	-0.14939444678792313
2108	35	-0.397703616448041
2108	36	-0.06993487336234239
2108	37	0.23735462845345978
2108	38	-0.1862060720631149
2108	39	-0.24448267685649228
2108	40	-0.1372347956913771
2108	41	-0.15350946754556105
2108	42	0.04522397358915242
2108	43	0.16859842649761703
2108	44	0.24463653852081535
2108	45	-0.012864441891643708
2108	46	-0.20223125120509167
2108	47	-0.37797927580130164
2108	48	-0.3752691543089668
2108	49	-0.2033631750737257
2108	50	0.2395058975927853
2108	51	-0.3159656850911503
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	1.0
16	1.0
17	1.0
18	1.0
19	7.0
20	14.0
21	30.0
22	89.0
23	283.0
24	688.0
25	1538.0
26	3084.0
27	5490.0
28	8635.0
29	12987.0
30	18362.0
31	25612.0
32	35207.0
33	50028.0
34	74730.0
35	128229.0
36	339883.0
37	2338971.0
38	41759.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	21.453317474875472	37.62337026798417	33.60179282674851	7.321519430391849
2	21.353305483807198	40.07295106671896	27.022812197185015	11.550931252288835
3	19.470416090069126	44.03437871682606	19.710593946779102	16.78461124632571
4	19.760049001338462	46.07736507617569	14.253005058934482	19.909580863551366
5	8.739803540930053	48.14216221646795	23.32907704423408	19.788957198367918
6	16.406989820555285	40.996814264834086	27.60632350605873	14.9898724085519
7	28.01820049714321	37.741822577561145	25.057476106986254	9.18250081830939
8	21.728237021289008	32.73221351879519	33.276964509678734	12.262584950237066
9	22.973460849162084	27.69223788983125	34.61636683594598	14.717934425060687
10	34.23177762725926	27.589211927548018	27.26969857047021	10.909311874722503
11	29.891205361627932	35.05183706406795	20.151930075867813	14.905027498436299
12	29.389784257995938	31.570732719088163	22.384731805174308	16.654751217741595
13	27.359696399114608	34.94404708276754	27.94849025968765	9.747766258430207
14	28.688598438568462	22.187981060593785	35.412314503035034	13.711105997802717
15	25.300927201252254	20.26403036008854	36.772296095124815	17.662746343534383
16	25.57169848620865	22.29162278043706	30.006902966331022	22.129775767023265
17	17.92739246118297	26.40695741226265	34.354702281219716	21.310947845334663
18	19.084887040896025	29.428576984278738	29.25402592015245	22.232510054672787
19	23.301367954032077	35.46465389563881	19.793915667140908	21.4400624831882
20	17.156010279910426	39.166426305162965	26.121958886839963	17.555604528086647
21	21.585543308821862	37.75676280046538	24.41893551722015	16.238758373492608
22	34.09222751917761	28.774383189170443	19.54916824116955	17.584221050482398
23	32.459724594329195	30.093271066200423	13.932098145273415	23.51490619419697
24	27.790985957486804	31.16323084750926	17.73582704342387	23.309956151580067
25	40.35982927311441	28.049442091242305	14.214406782407481	17.376321853235808
26	38.69621438733743	26.425559772234518	15.06039285332331	19.81783298710474
27	39.32564824687341	21.53706050304152	20.757154940806252	18.380136309278818
28	30.366570197982263	24.16799810735571	23.522522142965943	21.94290955169609
29	28.32105599180718	42.15644779186098	15.446310802008018	14.076185414323817
30	38.57993343336693	33.605163289182435	20.154166248059553	7.660737029391081
31	29.687810917057455	46.546183437417966	15.56398531256178	8.2020203329628
32	25.453181360046408	33.62856207646413	30.315332687328034	10.602923876161432
33	26.887831658364743	24.630788526168075	19.93061384547078	28.5507659699964
34	46.27343524661091	22.06463509882909	12.404889763192607	19.257039891367402
35	52.00056390429183	25.968700070974158	6.808074850192668	15.22266117454134
36	38.03871494638048	46.32583945579995	5.586347034479182	10.049098563340388
37	31.165402203115733	53.748894067013865	4.380920589960559	10.704783139909841
38	43.92678318528145	43.048388821731706	2.7147130407728732	10.310114952213972
39	34.4753583546958	49.18276008465046	4.1957396058503456	12.146141954803396
40	44.42318100355519	35.83307136630121	2.5001377352436944	17.243609894899908
41	29.530436248027147	31.089113082255487	3.8927868863084685	35.4876637834089
42	22.640854541860172	43.11177944212365	3.7794550869676535	30.467910929048525
43	15.055661242598756	35.72016087476463	4.681669545603329	44.54250833703328
44	14.781163003989462	45.78808865612533	5.6135051837064065	33.817243156178804
45	16.585851187601882	28.94339243525633	8.444693628205584	46.0260627489362
46	30.935011650781203	22.94704809066544	12.96208553844758	33.15585472010578
47	22.925172493137545	15.372030995291075	32.336670307198204	29.366126204373177
48	17.652667364525236	13.339058798365325	26.972773793358247	42.03550004375119
49	15.177289564853854	16.423518049798584	38.786471482322895	29.612720903024663
50	32.09263586366479	17.695122227875668	25.65621931339791	24.556022595061624
51	21.33100857847506	38.909817444087594	20.30479999222201	19.454373985215337
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1.0
1	1.0
2	1.0
3	1.0
4	1.0
5	2.0
6	3.0
7	2.5
8	2.0
9	5.0
10	8.0
11	9.0
12	10.0
13	23.0
14	36.0
15	41.0
16	46.0
17	59.0
18	72.0
19	82.0
20	92.0
21	115.5
22	139.0
23	143.0
24	147.0
25	171.0
26	274.5
27	354.0
28	524.0
29	694.0
30	899.5
31	1105.0
32	1593.0
33	2081.0
34	4834.5
35	7588.0
36	35421.0
37	63254.0
38	81491.5
39	99729.0
40	120046.0
41	140363.0
42	431395.5
43	722428.0
44	636279.5
45	550131.0
46	436495.5
47	322860.0
48	318340.0
49	313820.0
50	273440.5
51	233061.0
52	233027.0
53	232993.0
54	212880.0
55	192767.0
56	157445.5
57	122124.0
58	83807.5
59	45491.0
60	31054.5
61	16618.0
62	13371.0
63	10124.0
64	7160.5
65	4197.0
66	2991.0
67	1785.0
68	1286.0
69	787.0
70	481.5
71	176.0
72	167.0
73	158.0
74	138.0
75	77.5
76	37.0
77	25.5
78	14.0
79	8.5
80	3.0
81	5.0
82	7.0
83	3.5
84	0.0
85	1.5
86	3.0
87	2.0
88	1.0
89	1.0
90	1.0
91	0.5
92	0.0
93	0.5
94	1.0
95	1.0
96	1.0
97	1.0
98	1.0
99	1.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	3085630.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	16.92883648804234
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.55825752925773	11.436826951298595
2	15.787126695622536	5.345153728924042
3	5.919679609327095	3.006398645036903
4	2.9524593172226754	1.9992680407543921
5	1.5424638416245635	1.3056059081789937
6	1.126026391491426	1.143738999766722
7	0.7763026162138352	0.9199330038586452
8	0.5622659869243395	0.7614807164343915
9	0.4280983153357061	0.6522485743012101
>10	2.7756632068091562	9.099545828061363
>50	0.2845477701652279	3.362710490936178
>100	0.23235391944478692	7.8320437939963234
>500	0.02710898611369786	3.1130029932509036
>1k	0.020160404075141067	6.705198122750429
>5k	0.002111269592107313	2.4012980199047282
>10k+	0.005374140779909431	40.915546182546144
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TGAAAAAATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGTCT	227671	7.378428392257011	Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GAAAAAATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGTCTG	203872	6.607143435862368	Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAAAAATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGTCTGA	171828	5.568652106701063	Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AAAAATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGTCTGAA	102649	3.326678830579169	Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AAAATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGTCTGAAC	83955	2.720838208080684	Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TAGCCTGTGGGAATACTGCCAGCTGGGACTGAGGAAGATCGGAAGAGCACA	50721	1.6437810106850141	No Hit
TTGAAAAAATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGTC	46646	1.5117172182017935	No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTAGATCGGAAGAGCACACG	36411	1.1800183430936309	No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATTAGATCGGAAGAGCACACGT	36239	1.1744441167605968	No Hit
TTGATGATTCATAATAACTTTTCGAATCGCAAGATCGGAAGAGCACACGTC	29174	0.9454795293019578	No Hit
TTCCAGGGGGCATGCCTGTTTGAGCGTCATTAGATCGGAAGAGCACACGTC	28339	0.9184186049526353	No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCAGATCGGAAGAGCACACGTCTG	22417	0.726496695974566	Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGTCTGAACTCCAGT	20521	0.6650505731406553	Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AAATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGTCTGAACT	19657	0.6370498083049491	Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACAC	19495	0.6317996648982541	No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAATAGATCGGAAGAGCACACG	18811	0.6096323927366535	No Hit
ATGCCTGTTTGAGCGTCATTTCCTTCTCAAACAGATCGGAAGAGCACACGT	13984	0.45319756419272567	No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCAAGATCGGAAGAGCACACGT	13967	0.45264662321794896	No Hit
TTCCAGGGGGCATGCCTGTTTGAGCGTCATTTAGATCGGAAGAGCACACGT	13399	0.43423871300188294	No Hit
AATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGTCTGAACTC	12045	0.3903578847755564	Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TAGCCTGTGGGAATACTGCCAGCTGGGACTGAGGAGATCGGAAGAGCACAC	11850	0.3840382677119421	No Hit
TTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGTCTGAACTCCA	11784	0.3818993203981035	Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TTGCTCGAATATATTAGCATGGAATAGATCGGAAGAGCACACGTCTGAACT	11251	0.36462570042422454	Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TTTGAAAAAATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGT	10841	0.3513383004443177	No Hit
ATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGTCTGAACTCC	10644	0.3449538667954356	Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTCCAGGGGGCATGCCTGTTTGAGCGTCAGATCGGAAGAGCACACGTCTGA	10633	0.34459737557646253	Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATTCCAGGGGGCATGCCTGTTTGAGCGTAGATCGGAAGAGCACACGTCTGA	10246	0.3320553663271358	Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCCGATATAGTGTAACGGCTATCACATCACGAGATCGGAAGAGCACACGTC	10195	0.3304025434028059	No Hit
CTTTGAAAAAATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACG	8110	0.2628312532610845	No Hit
TTGCTCGAATATATTAGCATGGAATAATAGATCGGAAGAGCACACGTCTGA	7993	0.2590394830229159	Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCAGTCTCATAATCTGAAGGTCGAGAGTAGATCGGAAGAGCACACGTCTGA	7992	0.25900707473028195	Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCCGATATAGTGTAACGGCTATCACATCAGATCGGAAGAGCACACGTCTGA	7763	0.2515855757171145	Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTGCTCGAATATATTAGCATGGAATAATAGAAAGATCGGAAGAGCACACGT	7186	0.23288599086734313	No Hit
GCCAAGGACGTTTTCATTAATCAAGAACAGATCGGAAGAGCACACGTCTGA	7145	0.23155725086935244	Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTGCTCCACACAATATAGATGGATACGAATAAGGCGTCAGATCGGAAGAGC	6981	0.2262422908773897	No Hit
TGCCTGTTTGAGCGTCATTTCCTTCTCAAACAGATCGGAAGAGCACACGTC	5431	0.176009437294815	No Hit
AATACTGCCAGCTGGGACTGAGGACTGCGACGAGATCGGAAGAGCACACGT	5233	0.169592595353299	No Hit
GTCAAGGATGCTGGCATAATGGTTATATGCCAGATCGGAAGAGCACACGTC	5045	0.1634998363381222	No Hit
GGAATACTGCCAGCTGGGACTGAGGACTGCGACGAGATCGGAAGAGCACAC	5025	0.1628516704854438	No Hit
ATAGCCTGTGGGAATACTGCCAGCTGGGACTGAGGAAGATCGGAAGAGCAC	4940	0.16009696561156067	No Hit
TGATGATTCATAATAACTTTTCGAATCGCAAGATCGGAAGAGCACACGTCT	4564	0.14791144758120708	Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CATGCCTGTTTGAGCGTCATTTCCTTCTCAAACAGATCGGAAGAGCACACG	4321	0.14003623247116473	No Hit
ATCGAATCTTTGAACGCACATTGCGCCCCTTAGATCGGAAGAGCACACGTC	4305	0.13951769978902204	No Hit
GCCTGTTTGAGCGTCATTTCCTTCTCAAACAGATCGGAAGAGCACACGTCT	4090	0.1325499168727294	Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACA	4026	0.13047578614415858	No Hit
TAGCCTGTGGGAATACTGCCAGCTGGGACTAGATCGGAAGAGCACACGTCT	3953	0.12810998078188246	Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGTCTGAACTCCAG	3879	0.12571176712697246	Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AGCCTGTGGGAATACTGCCAGCTGGGACTGAGGAAGATCGGAAGAGCACAC	3819	0.12376726956893731	No Hit
TTCCAGGGGGCATGCCTGTTTGAGCGTAGATCGGAAGAGCACACGTCTGAA	3740	0.12120701445085767	Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACTGCCAGCTGGGACTGAGGACTGCGACGAGATCGGAAGAGCACACGTCTG	3579	0.1159892793367967	Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGAGCTACCAACTGTAGAAAGAGAAAGTGAGATCGGAAGAGCACACGTCTG	3521	0.1141095983640294	Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATCGAATCTTTGAACGCACATTGCGCCCCTAGATCGGAAGAGCACACGTCT	3507	0.11365588226715452	Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AATTCTAGAGCTAATACATGCTAGATCGGAAGAGCACACGTCTGAACTCCA	3469	0.11242436714706561	Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AGTTTTGGTGGCAGGATAAATCCAAGATCGGAAGAGCACACGTCTGAACTC	3193	0.1034796783801039	Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGAGGTGCACAATCGACCGATCCTGAAGATCGGAAGAGCACACGTCTGAAC	3163	0.10250742960108633	Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GATGATTCATAATAACTTTTCGAATCGCAAGATCGGAAGAGCACACGTCTG	3163	0.10250742960108633	Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA Adapter	Nextera Transposase Sequence
1	0.0016204146316959583	0.0	0.0
2	0.0017176395095977158	0.0	0.0
3	0.0017824560948655542	0.0	0.0
4	0.0018796809727673117	0.0	0.0
5	0.0018796809727673117	0.0	0.0
6	0.0018796809727673117	0.0	0.0
7	0.0019120892654012308	0.0	0.0
8	0.00194449755803515	0.0	0.0
9	0.001976905850669069	0.0	0.0
10	0.0020417224359369074	0.0	0.0
11	0.0022361721917404224	0.0	0.0
12	0.0026250717033474524	0.0	0.0
13	0.0031760126781240784	0.0	0.0
14	0.005088101943525309	0.0	0.0
15	0.009074321937497366	0.0	0.0
16	0.01750047802231635	0.0	0.0
17	0.03393148238771337	0.0	0.0
18	0.07285384184105029	0.0	0.0
19	0.18696344020507968	0.0	0.0
20	0.5197317889701617	0.0	0.0
21	1.8011556797153256	0.0	0.0
22	2.860355907869706	0.0	0.0
23	4.7034155099606885	0.0	0.0
24	6.643732398246063	0.0	0.0
25	8.993268797619935	0.0	0.0
26	12.060972961761456	0.0	0.0
27	17.198627184724028	0.0	0.0
28	24.81490003662137	0.0	0.0
29	49.79320268470296	0.0	0.0
30	66.02149317967482	0.0	0.0
31	76.6215003094992	0.0	0.0
32	82.41992721097475	0.0	0.0
33	86.92432339587053	0.0	0.0
34	90.19924618311333	0.0	0.0
35	92.0338796291195	0.0	0.0
36	94.39599044603533	0.0	0.0
37	95.10670430349718	0.0	0.0
38	95.48555724438769	0.0	0.0
39	95.84353924482197	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTGCTCG	5425	0.0	44.00461	1
ATTCCAG	12215	0.0	42.789604	1
TCAGTCT	1005	0.0	42.761192	1
GAGCTCC	100	0.0	42.75	2
CAGTCTC	1035	0.0	41.52174	2
TAGCCTG	8620	0.0	41.39791	1
TGCTCGA	5805	0.0	41.12403	2
TAATCGC	115	0.0	41.086956	10
ATAGCCT	710	0.0	40.88028	1
GCTCGAA	5920	0.0	40.287163	3
CTCGAAT	5925	0.0	40.253162	4
GGGTGCA	135	0.0	40.000004	2
CGATATA	2915	0.0	39.982845	3
ATATAGT	2920	0.0	39.83733	5
ATGTAGT	295	0.0	39.66102	5
TTATAGT	210	0.0	39.64286	2
GCGACTG	120	0.0	39.375004	2
GTCCTAT	280	0.0	39.375	2
CCGATAT	2985	0.0	39.195976	2
AATATAT	6125	0.0	39.19592	8
>>END_MODULE