FastQCFastQC Report
Wed 16 Sep 2015
000000000-AHBE6 l01n01 150915_gms7.341000000347ee.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AHBE6 l01n01 150915_gms7.341000000347ee.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3085630
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAAAAAATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGTCT2276717.378428392257011Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GAAAAAATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGTCTG2038726.607143435862368Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAAAAATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGTCTGA1718285.568652106701063Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AAAAATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGTCTGAA1026493.326678830579169Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AAAATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGTCTGAAC839552.720838208080684Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TAGCCTGTGGGAATACTGCCAGCTGGGACTGAGGAAGATCGGAAGAGCACA507211.6437810106850141No Hit
TTGAAAAAATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGTC466461.5117172182017935No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTAGATCGGAAGAGCACACG364111.1800183430936309No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATTAGATCGGAAGAGCACACGT362391.1744441167605968No Hit
TTGATGATTCATAATAACTTTTCGAATCGCAAGATCGGAAGAGCACACGTC291740.9454795293019578No Hit
TTCCAGGGGGCATGCCTGTTTGAGCGTCATTAGATCGGAAGAGCACACGTC283390.9184186049526353No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCAGATCGGAAGAGCACACGTCTG224170.726496695974566Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGTCTGAACTCCAGT205210.6650505731406553Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AAATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGTCTGAACT196570.6370498083049491Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACAC194950.6317996648982541No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAATAGATCGGAAGAGCACACG188110.6096323927366535No Hit
ATGCCTGTTTGAGCGTCATTTCCTTCTCAAACAGATCGGAAGAGCACACGT139840.45319756419272567No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCAAGATCGGAAGAGCACACGT139670.45264662321794896No Hit
TTCCAGGGGGCATGCCTGTTTGAGCGTCATTTAGATCGGAAGAGCACACGT133990.43423871300188294No Hit
AATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGTCTGAACTC120450.3903578847755564Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TAGCCTGTGGGAATACTGCCAGCTGGGACTGAGGAGATCGGAAGAGCACAC118500.3840382677119421No Hit
TTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGTCTGAACTCCA117840.3818993203981035Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TTGCTCGAATATATTAGCATGGAATAGATCGGAAGAGCACACGTCTGAACT112510.36462570042422454Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TTTGAAAAAATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGT108410.3513383004443177No Hit
ATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGTCTGAACTCC106440.3449538667954356Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTCCAGGGGGCATGCCTGTTTGAGCGTCAGATCGGAAGAGCACACGTCTGA106330.34459737557646253Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATTCCAGGGGGCATGCCTGTTTGAGCGTAGATCGGAAGAGCACACGTCTGA102460.3320553663271358Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCCGATATAGTGTAACGGCTATCACATCACGAGATCGGAAGAGCACACGTC101950.3304025434028059No Hit
CTTTGAAAAAATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACG81100.2628312532610845No Hit
TTGCTCGAATATATTAGCATGGAATAATAGATCGGAAGAGCACACGTCTGA79930.2590394830229159Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCAGTCTCATAATCTGAAGGTCGAGAGTAGATCGGAAGAGCACACGTCTGA79920.25900707473028195Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCCGATATAGTGTAACGGCTATCACATCAGATCGGAAGAGCACACGTCTGA77630.2515855757171145Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTGCTCGAATATATTAGCATGGAATAATAGAAAGATCGGAAGAGCACACGT71860.23288599086734313No Hit
GCCAAGGACGTTTTCATTAATCAAGAACAGATCGGAAGAGCACACGTCTGA71450.23155725086935244Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTGCTCCACACAATATAGATGGATACGAATAAGGCGTCAGATCGGAAGAGC69810.2262422908773897No Hit
TGCCTGTTTGAGCGTCATTTCCTTCTCAAACAGATCGGAAGAGCACACGTC54310.176009437294815No Hit
AATACTGCCAGCTGGGACTGAGGACTGCGACGAGATCGGAAGAGCACACGT52330.169592595353299No Hit
GTCAAGGATGCTGGCATAATGGTTATATGCCAGATCGGAAGAGCACACGTC50450.1634998363381222No Hit
GGAATACTGCCAGCTGGGACTGAGGACTGCGACGAGATCGGAAGAGCACAC50250.1628516704854438No Hit
ATAGCCTGTGGGAATACTGCCAGCTGGGACTGAGGAAGATCGGAAGAGCAC49400.16009696561156067No Hit
TGATGATTCATAATAACTTTTCGAATCGCAAGATCGGAAGAGCACACGTCT45640.14791144758120708Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CATGCCTGTTTGAGCGTCATTTCCTTCTCAAACAGATCGGAAGAGCACACG43210.14003623247116473No Hit
ATCGAATCTTTGAACGCACATTGCGCCCCTTAGATCGGAAGAGCACACGTC43050.13951769978902204No Hit
GCCTGTTTGAGCGTCATTTCCTTCTCAAACAGATCGGAAGAGCACACGTCT40900.1325499168727294Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACA40260.13047578614415858No Hit
TAGCCTGTGGGAATACTGCCAGCTGGGACTAGATCGGAAGAGCACACGTCT39530.12810998078188246Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGTCTGAACTCCAG38790.12571176712697246Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AGCCTGTGGGAATACTGCCAGCTGGGACTGAGGAAGATCGGAAGAGCACAC38190.12376726956893731No Hit
TTCCAGGGGGCATGCCTGTTTGAGCGTAGATCGGAAGAGCACACGTCTGAA37400.12120701445085767Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACTGCCAGCTGGGACTGAGGACTGCGACGAGATCGGAAGAGCACACGTCTG35790.1159892793367967Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGAGCTACCAACTGTAGAAAGAGAAAGTGAGATCGGAAGAGCACACGTCTG35210.1141095983640294Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATCGAATCTTTGAACGCACATTGCGCCCCTAGATCGGAAGAGCACACGTCT35070.11365588226715452Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AATTCTAGAGCTAATACATGCTAGATCGGAAGAGCACACGTCTGAACTCCA34690.11242436714706561Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AGTTTTGGTGGCAGGATAAATCCAAGATCGGAAGAGCACACGTCTGAACTC31930.1034796783801039Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGAGGTGCACAATCGACCGATCCTGAAGATCGGAAGAGCACACGTCTGAAC31630.10250742960108633Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GATGATTCATAATAACTTTTCGAATCGCAAGATCGGAAGAGCACACGTCTG31630.10250742960108633Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGCTCG54250.044.004611
ATTCCAG122150.042.7896041
TCAGTCT10050.042.7611921
GAGCTCC1000.042.752
CAGTCTC10350.041.521742
TAGCCTG86200.041.397911
TGCTCGA58050.041.124032
TAATCGC1150.041.08695610
ATAGCCT7100.040.880281
GCTCGAA59200.040.2871633
CTCGAAT59250.040.2531624
GGGTGCA1350.040.0000042
CGATATA29150.039.9828453
ATATAGT29200.039.837335
ATGTAGT2950.039.661025
TTATAGT2100.039.642862
GCGACTG1200.039.3750042
GTCCTAT2800.039.3752
CCGATAT29850.039.1959762
AATATAT61250.039.195928
Produced by FastQC (version 0.11.2)