##FastQC	0.11.2
>>Basic Statistics	pass
#Measure	Value
Filename	000000000-AHBE6 l01n01 150915_gms6.341000000347d1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3173971
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.078846341066125	33.0	33.0	34.0	32.0	35.0
2	33.352911226977184	34.0	33.0	34.0	32.0	35.0
3	33.45730947132157	34.0	33.0	34.0	32.0	35.0
4	33.544189912258176	34.0	33.0	34.0	32.0	35.0
5	33.107377477614	34.0	33.0	34.0	32.0	35.0
6	36.08910005794004	37.0	37.0	37.0	34.0	37.0
7	35.940030643002096	37.0	36.0	37.0	33.0	37.0
8	35.7309159409459	37.0	35.0	37.0	33.0	37.0
9	36.03261371953304	37.0	37.0	37.0	34.0	37.0
10	36.119520940802545	37.0	37.0	37.0	34.0	37.0
11	35.67992996785415	37.0	34.0	37.0	33.0	37.0
12	35.71557049513055	37.0	35.0	37.0	33.0	37.0
13	37.50265771174343	38.0	38.0	38.0	37.0	38.0
14	37.58746787541537	38.0	38.0	38.0	38.0	38.0
15	37.48424796571865	38.0	38.0	38.0	37.0	38.0
16	37.49091689873663	38.0	38.0	38.0	37.0	38.0
17	37.52412671697378	38.0	38.0	38.0	37.0	38.0
18	37.46001680544655	38.0	38.0	38.0	37.0	38.0
19	37.4941138403596	38.0	38.0	38.0	37.0	38.0
20	37.51902585121288	38.0	38.0	38.0	37.0	38.0
21	37.52624173314753	38.0	38.0	38.0	37.0	38.0
22	37.54986261689221	38.0	38.0	38.0	37.0	38.0
23	38.17480310941719	39.0	38.0	39.0	37.0	39.0
24	38.155118304483565	39.0	38.0	39.0	37.0	39.0
25	38.146571912597814	39.0	38.0	39.0	37.0	39.0
26	38.03152958864464	39.0	38.0	39.0	37.0	39.0
27	37.73393298174432	38.0	38.0	39.0	37.0	39.0
28	37.337621547266814	38.0	38.0	39.0	36.0	39.0
29	37.63694186241777	38.0	38.0	39.0	36.0	39.0
30	37.68055410714213	38.0	38.0	39.0	36.0	39.0
31	37.78659666392667	38.0	38.0	39.0	37.0	39.0
32	37.785592243911495	38.0	38.0	39.0	37.0	39.0
33	37.88292363099726	39.0	38.0	39.0	37.0	39.0
34	37.94259588383132	39.0	38.0	39.0	37.0	39.0
35	37.94574115516493	39.0	38.0	39.0	37.0	39.0
36	37.161580556344084	38.0	38.0	39.0	35.0	39.0
37	37.39677867252095	38.0	38.0	39.0	36.0	39.0
38	37.638839485300906	38.0	38.0	39.0	36.0	39.0
39	37.85764331180089	38.0	38.0	39.0	37.0	39.0
40	37.88590569983153	38.0	38.0	39.0	37.0	39.0
41	37.9071393531951	39.0	38.0	39.0	37.0	39.0
42	37.67023107646541	39.0	38.0	39.0	37.0	39.0
43	37.802007012666465	39.0	38.0	39.0	37.0	39.0
44	37.677784390594624	39.0	38.0	39.0	37.0	39.0
45	37.956346797119444	39.0	38.0	39.0	37.0	39.0
46	38.0104055771146	39.0	38.0	39.0	37.0	39.0
47	37.98256852378298	39.0	38.0	39.0	37.0	39.0
48	37.941349810694554	39.0	38.0	39.0	37.0	39.0
49	38.049345126341734	39.0	38.0	39.0	37.0	39.0
50	37.68877692959388	39.0	38.0	39.0	37.0	39.0
51	37.76933532158927	39.0	38.0	39.0	37.0	39.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	-0.06741007290661827
1101	2	0.1086443013686278
1101	3	0.14259837118346042
1101	4	0.17954819144456025
1101	5	0.04288773128938317
1101	6	0.04672231955944994
1101	7	0.04209759591596196
1101	8	0.09988901522634563
1101	9	0.08678031824631915
1101	10	0.022135999621333724
1101	11	0.1196758356266372
1101	12	0.093241367194004
1101	13	0.011782149958250443
1101	14	0.005569253376151551
1101	15	0.03227566664772752
1101	16	0.03983762803932933
1101	17	-0.021911227142226153
1101	18	0.052293666429882535
1101	19	0.004907692219703108
1101	20	0.040028677709557314
1101	21	0.02883126066824815
1101	22	0.05376638464352368
1101	23	0.13317257656649417
1101	24	0.04126908138109542
1101	25	0.04470444450194577
1101	26	0.07352808615745232
1101	27	0.035144137440688894
1101	28	0.4159672308100326
1101	29	0.12458794035163834
1101	30	0.16583250064934418
1101	31	0.08246144289479673
1101	32	0.09790183066881752
1101	33	0.13213359344931774
1101	34	0.0969942189736841
1101	35	0.16916817414733742
1101	36	0.7776136613684841
1101	37	0.5064187787736856
1101	38	0.21089532227562557
1101	39	0.111536164286413
1101	40	0.057994866916757815
1101	41	0.02692368938065215
1101	42	0.24815137384791086
1101	43	0.17249977415892914
1101	44	0.3649838637808145
1101	45	0.13739925142140663
1101	46	0.10726536768055439
1101	47	0.08845863949532884
1101	48	0.14113145632898494
1101	49	-0.0029312469150326592
1101	50	0.4957690346060062
1101	51	0.10495641752384444
1102	1	-0.08469105725036741
1102	2	0.08893853106004457
1102	3	0.0341358438087056
1102	4	0.125256703916925
1102	5	0.07994244327814926
1102	6	0.02319495382207748
1102	7	0.09275016902672917
1102	8	0.031311568098495
1102	9	-0.02536046085653254
1102	10	-0.060067989547391676
1102	11	-0.027028891208011885
1102	12	0.09619029542886182
1102	13	0.05536712963316148
1102	14	0.01857489310435767
1102	15	0.025366823011225392
1102	16	-8.911340142674362E-4
1102	17	-0.08944599194773417
1102	18	0.014405198302725353
1102	19	0.041559196768339746
1102	20	0.017179241207408324
1102	21	0.13284139508006376
1102	22	0.07474081368619778
1102	23	0.06767044248690723
1102	24	-0.0065570815601319055
1102	25	0.03828442480649841
1102	26	0.148500312554269
1102	27	0.1502502251758031
1102	28	0.45461236368854685
1102	29	0.15881392821138718
1102	30	0.22057317033844726
1102	31	0.0979480734458349
1102	32	0.17570996664779415
1102	33	0.09780892739630076
1102	34	0.13188388694972986
1102	35	0.17899345924541166
1102	36	0.7906011547828342
1102	37	0.5280086601455096
1102	38	0.33408568417158335
1102	39	0.17489962131265457
1102	40	0.14420133616289377
1102	41	0.2107301076709689
1102	42	0.23449115760718087
1102	43	0.21755198682788546
1102	44	0.40825158743169254
1102	45	0.15347423661815185
1102	46	0.08079589379943286
1102	47	0.13112385072207644
1102	48	0.08675149029790674
1102	49	0.06399665156888545
1102	50	0.43423531059512044
1102	51	0.203858516820965
1103	1	0.15220369507027698
1103	2	0.115086625103622
1103	3	-0.018270333494029956
1103	4	0.06059589225371553
1103	5	-0.032722451167330746
1103	6	0.13306073464758583
1103	7	-0.5703157446065532
1103	8	-0.9827774115603418
1103	9	-0.9095167832623474
1103	10	-0.25111466377257585
1103	11	-0.12056470873907443
1103	12	0.027351805483178282
1103	13	0.1060949688113908
1103	14	0.14790047237415394
1103	15	0.12179334707532519
1103	16	0.05725084253980839
1103	17	0.07877941226574592
1103	18	0.051311274414146624
1103	19	0.17998543544493373
1103	20	0.1650799176504094
1103	21	0.17370227209017486
1103	22	0.04073481712887883
1103	23	0.050969949525068614
1103	24	-0.17388483636563734
1103	25	0.06521488631664596
1103	26	0.04998529131653129
1103	27	0.221485900230725
1103	28	0.4841080640677262
1103	29	0.19538513254977374
1103	30	0.2210649819242505
1103	31	0.16638596389122995
1103	32	0.21974859313181128
1103	33	0.11599602346650073
1103	34	0.17858833740935154
1103	35	0.2056830123528286
1103	36	0.8242612355680166
1103	37	0.6069537693715148
1103	38	0.38829943226394903
1103	39	0.19190627631304125
1103	40	0.13744611594974998
1103	41	0.25129829150279903
1103	42	0.3865435383389837
1103	43	0.18282813810066756
1103	44	0.4204627297309429
1103	45	0.17993931908364402
1103	46	0.07032923703946636
1103	47	0.19968097128026585
1103	48	0.16477397093710522
1103	49	0.1989677273274566
1103	50	0.509332654781474
1103	51	0.2918922466398257
2112	1	-0.09769980605989304
2112	2	0.0441429749825204
2112	3	0.052348057856946184
2112	4	0.1107916798930404
2112	5	0.1796389017021056
2112	6	0.1108255192756431
2112	7	-0.4360038094474632
2112	8	-0.02724736742769096
2112	9	-0.003629208541511275
2112	10	-0.00961632698808046
2112	11	0.011821799923502851
2112	12	0.004069550661732535
2112	13	0.02911703877764893
2112	14	0.03542703281252102
2112	15	0.05280956782896595
2112	16	-0.22498002929473415
2112	17	-0.009641016194777308
2112	18	0.05348510091309322
2112	19	-0.03446519215585653
2112	20	0.0881118096463851
2112	21	0.11412383423928674
2112	22	0.13728885178679207
2112	23	0.14293529176898545
2112	24	-0.2520784345075384
2112	25	-0.012889110537138038
2112	26	0.0673785863605687
2112	27	0.13967451850066936
2112	28	0.42430271437774536
2112	29	0.21364046305870232
2112	30	0.20353004703812871
2112	31	0.16047005487028798
2112	32	0.13413571391024703
2112	33	0.13602294421744432
2112	34	0.06984654100047294
2112	35	0.1300652395285411
2112	36	0.7436565151211099
2112	37	0.5509596204836313
2112	38	0.27846027532176265
2112	39	0.1857191573866288
2112	40	0.08619174144644148
2112	41	0.06612308224251962
2112	42	0.3327859787991656
2112	43	-0.047765956983703006
2112	44	0.19080686405198577
2112	45	0.20042944232439197
2112	46	0.0795954633531224
2112	47	0.0940366916390829
2112	48	0.11941298839222014
2112	49	0.15005267379660125
2112	50	0.48131651404452924
2112	51	0.2220426170870553
1113	1	-0.02525102573547855
1113	2	8.659309669951654E-4
1113	3	0.10895019863966127
1113	4	0.13409665051581499
1113	5	0.10369200356102226
1113	6	0.08919599454498695
1113	7	0.10141879456352854
1113	8	0.10615748308554629
1113	9	0.13196100413342293
1113	10	0.08982364955360822
1113	11	0.10176540334403938
1113	12	0.08913564195429302
1113	13	0.044020387321907606
1113	14	0.037776133656400646
1113	15	0.025649740292536194
1113	16	0.10660395110159726
1113	17	0.06702237099378294
1113	18	0.06921050655621741
1113	19	0.06947067083073932
1113	20	-0.0036501846413656835
1113	21	0.09644377308508467
1113	22	-0.021304564012872618
1113	23	0.048205772029760396
1113	24	0.0817202168276765
1113	25	0.00415700129602925
1113	26	0.05506845301786001
1113	27	-0.001806899522897254
1113	28	0.3816211372117593
1113	29	0.12448607961587044
1113	30	0.0802681269161667
1113	31	0.11913317663329792
1113	32	0.137830595708607
1113	33	0.15011712587945425
1113	34	0.08441241520579013
1113	35	0.06863164326771454
1113	36	0.8021258320069009
1113	37	0.5266353583344525
1113	38	0.2818425693672637
1113	39	0.06325198498124962
1113	40	0.09279684839030722
1113	41	0.03006458305232229
1113	42	0.15086470518554762
1113	43	0.03498669326130255
1113	44	0.22888287032039756
1113	45	0.010262217167920085
1113	46	0.0591160376207398
1113	47	0.06928644076207036
1113	48	0.06054435705895145
1113	49	0.03532490035241409
1113	50	0.3275643389968934
1113	51	0.18645891538285042
2113	1	-0.3799881888375509
2113	2	-0.038205564868384556
2113	3	0.05199552725143519
2113	4	0.12133503687871894
2113	5	0.12724916267255537
2113	6	0.12886711642947546
2113	7	0.13148284411291655
2113	8	0.058560063075816515
2113	9	0.11700791664816279
2113	10	0.06332320744206754
2113	11	0.0641407272035508
2113	12	0.06601198748023052
2113	13	0.11885809487987586
2113	14	0.06568487926923439
2113	15	0.06769583386194
2113	16	0.10435714216708192
2113	17	0.07621319896394851
2113	18	0.06568385886748729
2113	19	0.01638829396159025
2113	20	0.032408894181841674
2113	21	0.049931055504892186
2113	22	0.09885664460948362
2113	23	0.010173911425113147
2113	24	0.12338751362205613
2113	25	0.05747649203017602
2113	26	0.10358631248667649
2113	27	1.8416467372617262E-4
2113	28	0.36540990827315056
2113	29	0.17159380037272598
2113	30	0.21006788522124964
2113	31	0.0659595735323606
2113	32	-0.005346129341219807
2113	33	0.053067073714167634
2113	34	0.026427865403064743
2113	35	-0.018905001998298587
2113	36	0.797824400187146
2113	37	0.5454087504465619
2113	38	0.2564570758155824
2113	39	0.14194052206257624
2113	40	0.044863352522355626
2113	41	0.038725941128198826
2113	42	0.24975624026237853
2113	43	0.08258248713499228
2113	44	0.45141765911981935
2113	45	0.13493767235650012
2113	46	0.08231026639889194
2113	47	0.07797300394654627
2113	48	0.04101859826264587
2113	49	0.0318550745029853
2113	50	0.39811545903409495
2113	51	0.10572047121080885
1112	1	0.13306111797711395
1112	2	0.08000800925733387
1112	3	0.1362198824182954
1112	4	-0.025399411621911838
1112	5	0.026915748100925896
1112	6	-0.002888207324794223
1112	7	0.004253669485876799
1112	8	-0.0823209079863716
1112	9	0.015853997608004988
1112	10	0.06118595042832453
1112	11	0.1267095292002125
1112	12	0.04120679158008045
1112	13	0.0270260554791264
1112	14	-0.012056988131242008
1112	15	0.1537830954981274
1112	16	0.09417943066513601
1112	17	0.0940243992591121
1112	18	0.11673060382284461
1112	19	0.01929649799282629
1112	20	-0.04783207472369355
1112	21	0.013874320747554236
1112	22	-0.06601480900656043
1112	23	0.04931146126989461
1112	24	0.0949688355217333
1112	25	0.05253280144196992
1112	26	-0.21503326575998472
1112	27	0.021650298597407414
1112	28	0.3153160816411855
1112	29	0.12092110152449465
1112	30	0.15397031391648852
1112	31	0.11131762479990925
1112	32	0.19152856767489368
1112	33	0.10769382384763304
1112	34	0.08379314278337802
1112	35	0.09503226966512557
1112	36	0.7788076245060864
1112	37	0.4868687810848513
1112	38	0.2923281540074143
1112	39	0.12275659353542778
1112	40	0.10019336786782418
1112	41	0.1465084111859838
1112	42	0.2315094902839192
1112	43	0.24851661167962646
1112	44	0.48094520551013886
1112	45	0.07926353428278787
1112	46	-0.03884375003741525
1112	47	0.041117241422632844
1112	48	0.04496897203041783
1112	49	-0.025323446927657756
1112	50	0.32149066659415126
1112	51	0.15073613398991625
2114	1	0.09551549653632918
2114	2	0.13814161391238855
2114	3	-0.07470346697421348
2114	4	0.05104032870764286
2114	5	0.005749957768799163
2114	6	-0.018354456898656224
2114	7	0.045228028801957976
2114	8	0.018397929135353763
2114	9	0.04354564059414656
2114	10	0.08203807097562077
2114	11	0.05819591558842063
2114	12	0.06596754110633185
2114	13	0.013439427265019788
2114	14	0.006978248076976001
2114	15	0.03158236261391778
2114	16	0.07247873274545924
2114	17	0.001935429570110614
2114	18	0.11086592936104012
2114	19	-0.04034905277594447
2114	20	0.057620163760930154
2114	21	0.02701241487943662
2114	22	0.02828271673756433
2114	23	0.07204167898367331
2114	24	0.1460884933487705
2114	25	0.007314268507869315
2114	26	0.008010483871892404
2114	27	-0.005105665881075083
2114	28	0.42906497133532184
2114	29	-0.00809186384559979
2114	30	0.16824269889676202
2114	31	0.07242965629908582
2114	32	0.04914204711938197
2114	33	0.010725413634062875
2114	34	0.05409273560931638
2114	35	0.1369846019374208
2114	36	0.8293034583650396
2114	37	0.5214603091590746
2114	38	0.2777978816524609
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2108	4	-0.2454760143727981
2108	5	-0.24733635990807556
2108	6	-0.015836900697287604
2108	7	-0.020840049467352628
2108	8	0.06919701503529296
2108	9	-0.020400478250252263
2108	10	-0.013981543955289055
2108	11	-0.17569728467060486
2108	12	-0.05745703595839302
2108	13	-0.34692755206614123
2108	14	-0.09734921804230368
2108	15	-0.19742056632374982
2108	16	-0.07756870105733782
2108	17	-0.04306866921155006
2108	18	-0.26957391922433516
2108	19	-0.12747475565607402
2108	20	-0.12045901891536204
2108	21	-0.186734368106535
2108	22	-0.1658712749108986
2108	23	-0.06033812672109207
2108	24	-0.06451141841183272
2108	25	-0.24042076456140649
2108	26	-0.07277394025668826
2108	27	-0.17229008417865543
2108	28	0.16591480650712498
2108	29	0.014048048675761038
2108	30	0.056659603415766924
2108	31	-0.083363018919556
2108	32	-0.11649437785013106
2108	33	-0.2835263383416944
2108	34	-0.18339500164226763
2108	35	-0.3356686092456158
2108	36	0.02473733131624556
2108	37	0.19328034815843154
2108	38	-0.1617993558210884
2108	39	-0.22544257865319395
2108	40	-0.1270950479742936
2108	41	-0.20628609537180864
2108	42	0.11236704721751067
2108	43	0.13848173054282142
2108	44	0.27271084317325034
2108	45	0.016906227041964428
2108	46	-0.08996975363274373
2108	47	-0.4170973345842057
2108	48	-0.3266263752135856
2108	49	-0.16876701950426565
2108	50	0.28544865242874096
2108	51	-0.2962957049924029
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	1.0
17	0.0
18	0.0
19	4.0
20	12.0
21	19.0
22	84.0
23	254.0
24	686.0
25	1638.0
26	3029.0
27	5451.0
28	8807.0
29	13249.0
30	19194.0
31	26518.0
32	35951.0
33	50094.0
34	74178.0
35	128177.0
36	334613.0
37	2444979.0
38	27033.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	17.146407449847526	37.53981999205412	41.53645386173976	3.7773186963585994
2	21.856941982141613	46.40669369694934	24.556651588814137	7.179712732094906
3	22.15256535110119	51.85173399504911	17.423757179886014	8.571943473963687
4	9.880083970521468	54.12094187376003	10.095964959982307	25.903009195736193
5	5.39337000873669	53.55130843980616	18.988390253093048	22.0669312983641
6	12.630455665789006	51.87927047852674	27.343318511731834	8.146955343952419
7	29.268855953630325	44.17907409992089	21.856154325291566	4.695915621157219
8	20.4792986451357	36.65411561731345	37.23361051502991	5.632975222520937
9	29.534894931302148	28.96964086943454	35.30290604419511	6.19255815506821
10	39.394310786078385	26.614861950534518	27.954666252464182	6.0361610109229105
11	39.15133440097594	37.72699876589925	14.622156283091433	8.49951055003338
12	33.800119786853756	38.02747410105511	16.22160378907054	11.950802323020595
13	24.74622483948341	43.71498668387329	27.094734009857053	4.444054466786244
14	32.35332017841373	15.117939010784912	46.26513600785893	6.263604802942433
15	27.317294329406288	21.434537366598498	39.082965786391874	12.165202517603344
16	24.896320728828336	16.401000513237204	31.050850811176282	27.65182794675818
17	14.932461575735884	20.121702435214438	44.30229513754222	20.643540851507463
18	22.866686557627652	25.705496364018448	31.93277443303672	19.495042645317177
19	19.784427772024383	33.93055576122151	15.525315133629135	30.759701333124973
20	14.553126036753328	42.83734791527711	21.69610245336205	20.91342359460751
21	20.121387372474416	48.31105892271857	20.511056969329587	11.05649673547742
22	37.283201390308854	28.839299413888785	17.91339618414913	15.964103011653224
23	37.682259856816586	24.122337601698316	8.313245458134306	29.88215708335079
24	26.97129242831771	30.998896965347196	16.725420616634494	25.3043899897006
25	48.68390416925675	27.0063904175558	9.373746641037362	14.935958772150093
26	50.549296134085665	19.19573304229938	9.860896649654329	20.394074173960632
27	52.975184713407906	17.148801926671666	13.674951661499113	16.201061698421316
28	26.365237741617676	23.778793189981887	24.335193988854968	25.520775079545466
29	29.511233719526736	28.80505209404875	17.795940794670145	23.887773391754365
30	27.456867123234584	31.274419331493576	34.010959772474294	7.257753772797545
31	34.683398178496276	37.12261391172131	19.258619565207116	8.935368344575297
32	30.772587399191735	37.935853856257665	20.627598676862515	10.663960067688079
33	33.32182304123131	29.974344441080277	21.863400768311998	14.840431749376412
34	37.71181274183034	24.65895246049822	20.695841266350577	16.93339353132086
35	39.03255574798888	32.80779818089075	8.733759697237309	19.425886373883063
36	45.39430259444715	37.679802367444445	7.64093307720833	9.284961960900084
37	40.74110948083647	41.27967772862449	8.404393108821726	9.574819681717319
38	32.528652593234156	48.802052696763774	7.175207334912638	11.494087375089439
39	35.85231245023978	41.08077862085066	8.778152037305949	14.28875689160361
40	44.381722454300935	35.524993769634314	2.1728931990872002	17.920390576977546
41	37.64785500560654	39.968103048200504	1.9810830029637954	20.40295894322916
42	34.6148090199942	38.83403471550307	2.0217576027002138	24.529398661802517
43	19.061642340147404	42.695790226186695	2.7161873879755047	35.526380045690395
44	19.71810706525044	46.69163643902228	3.578293563488765	30.01196293223851
45	23.252669920424605	31.607377635145372	7.002111865546345	38.137840578883676
46	21.63570492610046	34.041993452366135	9.114481512275947	35.20782010925746
47	25.270678276518595	19.218921659964757	17.716355946541416	37.794044116975236
48	23.791679256048653	17.483209518927552	22.148406522932945	36.57670470209085
49	14.25951906932987	23.53496613548139	30.238209485845964	31.967305309342777
50	19.34258378542211	20.065526748669097	23.728603695496904	36.86328577041189
51	22.83940212434203	27.804727894489268	26.068039058958004	23.287830922210695
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.5
2	1.0
3	1.0
4	1.0
5	1.5
6	2.0
7	4.5
8	7.0
9	13.5
10	20.0
11	21.5
12	23.0
13	25.5
14	28.0
15	46.5
16	65.0
17	83.5
18	102.0
19	117.5
20	133.0
21	159.0
22	185.0
23	194.5
24	204.0
25	236.0
26	341.5
27	415.0
28	533.5
29	652.0
30	850.5
31	1049.0
32	1665.5
33	2282.0
34	5888.5
35	9495.0
36	52649.0
37	95803.0
38	96279.0
39	96755.0
40	141689.0
41	186623.0
42	603098.0
43	1019573.0
44	795275.0
45	570977.0
46	348048.0
47	125119.0
48	124139.0
49	123159.0
50	111944.0
51	100729.0
52	182582.5
53	264436.0
54	281429.0
55	298422.0
56	243667.5
57	188913.0
58	123583.5
59	58254.0
60	36745.0
61	15236.0
62	11877.5
63	8519.0
64	5995.0
65	3471.0
66	2610.5
67	1750.0
68	1315.5
69	881.0
70	532.0
71	183.0
72	144.0
73	105.0
74	90.5
75	47.5
76	19.0
77	14.5
78	10.0
79	11.0
80	12.0
81	7.0
82	2.0
83	2.5
84	3.0
85	2.5
86	2.0
87	1.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.5
94	1.0
95	1.0
96	1.0
97	2.5
98	4.0
99	2.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	3173971.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	7.0038794086943135
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.21608389183851	5.127966223552831
2	11.719778905477488	1.6416783630104754
3	4.571428565651906	0.960532031978591
4	2.3389838107423113	0.6552784219730973
5	1.6791761690278522	0.5880373696912187
6	1.1843953398553508	0.49772172795398373
7	0.7602703457585901	0.37273892737896464
8	0.5664535071193703	0.3173897643596828
9	0.36435478234025026	0.2296707261742959
>10	2.648187749475989	3.6909778135074975
>50	0.43449178441560454	2.163978795323512
>100	0.3914025970309931	5.8195471801337755
>500	0.05706511572379842	2.8357693727733175
>1k	0.04890775379562977	7.061798446079827
>5k	0.00724559304624229	3.6001374883186394
>10k+	0.011774088700143721	64.43677734779033
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TGAAAAAATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGTCT	412241	12.988177900806278	Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GAAAAAATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGTCTG	307107	9.675797289893323	Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAAAAATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGTCTGA	250479	7.891660005715238	Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TAGCCTGTGGGAATACTGCCAGCTGGGACTGAGGAAGATCGGAAGAGCACA	166375	5.241856337061681	No Hit
AAAAATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGTCTGAA	134402	4.234506238399784	Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AAAATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGTCTGAAC	109877	3.4618148685038395	Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTAGATCGGAAGAGCACACG	102373	3.225391788393782	No Hit
TTGAAAAAATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGTC	78631	2.477369831041304	No Hit
ACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACAC	67269	2.119395545832019	No Hit
TAGCCTGTGGGAATACTGCCAGCTGGGACTGAGGAGATCGGAAGAGCACAC	66067	2.0815250044817675	No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATTAGATCGGAAGAGCACACGT	50989	1.606473405081521	No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAATAGATCGGAAGAGCACACG	39316	1.2387006686576532	No Hit
TTCCAGGGGGCATGCCTGTTTGAGCGTCATTTAGATCGGAAGAGCACACGT	25395	0.800101828277574	No Hit
AAATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGTCTGAACT	23672	0.7458165181723463	Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TTCCAGGGGGCATGCCTGTTTGAGCGTCATTAGATCGGAAGAGCACACGTC	23417	0.7377824183018685	No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCAGATCGGAAGAGCACACGTCTG	18633	0.5870564034769064	Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTGATGATTCATAATAACTTTTCGAATCGCAAGATCGGAAGAGCACACGTC	18473	0.5820153996366066	No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCAAGATCGGAAGAGCACACGT	18196	0.5732881617380877	No Hit
TTTGAAAAAATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGT	17915	0.5644348987435612	No Hit
CTTTGAAAAAATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACG	17268	0.5440503394643492	No Hit
TACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACA	16317	0.5140878728885676	No Hit
TAGCCTGTGGGAATACTGCCAGCTGGGACTAGATCGGAAGAGCACACGTCT	15519	0.48894586623507275	Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TAGCCTGTGGGAATACTGCCAGCTGGGACTGAGATCGGAAGAGCACACGTC	14125	0.44502612027646127	No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAAAGATCGGAAGAGCACACGT	13795	0.43462904985584305	No Hit
AATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGTCTGAACTC	11895	0.3747671292522836	Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGCCTGTGGGAATACTGCCAGCTGGGACTGAGGAAGATCGGAAGAGCACAC	11868	0.3739164598542331	No Hit
TCCGATATAGTGTAACGGCTATCACATCACGAGATCGGAAGAGCACACGTC	9683	0.3050752511601398	No Hit
GGAATACTGCCAGCTGGGACTGAGGACTGCGACGAGATCGGAAGAGCACAC	9536	0.3004438288818644	No Hit
ATAGCCTGTGGGAATACTGCCAGCTGGGACTGAGGAAGATCGGAAGAGCAC	8902	0.2804688511646767	No Hit
ATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGTCTGAACTCC	8225	0.2591391036654084	Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AATACTGCCAGCTGGGACTGAGGACTGCGACGAGATCGGAAGAGCACACGT	7803	0.24584345603661786	No Hit
GGGAATACTGCCAGCTGGGACTGAGGACTGCGACGAGATCGGAAGAGCACA	7582	0.23888056948220385	No Hit
TTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGTCTGAACTCCA	7528	0.23717923068610267	Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ATGCCTGTTTGAGCGTCATTTCCTTCTCAAACAGATCGGAAGAGCACACGT	7093	0.22347400149528776	No Hit
AGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGTCTGAACTCCAGT	7033	0.22158362505517534	Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TAGCCTGTGGGAATACTGCCAGCTGGGACTGAGAGATCGGAAGAGCACACG	6728	0.211974211484604	No Hit
TTACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCAC	6249	0.19688270623770665	No Hit
TTGCTCGAATATATTAGCATGGAATAATAGATCGGAAGAGCACACGTCTGA	5878	0.18519387858301162	Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATAGCCTGTGGGAATACTGCCAGCTGGGACTGAGGAGATCGGAAGAGCACA	5855	0.18446923428096854	No Hit
TTCCAGGGGGCATGCCTGTTTGAGCGTCAGATCGGAAGAGCACACGTCTGA	5234	0.1649038381258052	Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATTCCAGGGGGCATGCCTGTTTGAGCGTAGATCGGAAGAGCACACGTCTGA	5129	0.16159567935560848	Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTGCTCGAATATATTAGCATGGAATAGATCGGAAGAGCACACGTCTGAACT	5050	0.1591066837094605	Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGCCTGTGGGAATACTGCCAGCTGGGACTGAGGAGATCGGAAGAGCACACG	4882	0.15381362967714576	No Hit
TCAGTCTCATAATCTGAAGGTCGAGAGTAGATCGGAAGAGCACACGTCTGA	4516	0.1422823333924601	Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCTGAGATTAAGCCTTTGTTGTCTGATTTGTAGATCGGAAGAGCACACGTC	4466	0.14070701969236643	No Hit
TTGCTCCACACAATATAGATGGATACGAATAAGGCGTCAGATCGGAAGAGC	4422	0.13932074363628402	No Hit
GAGATTAAGCCTTTGTTGTCTGATTTGTAGATCGGAAGAGCACACGTCTGA	4135	0.13027844299774635	Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACTGCCAGCTGGGACTGAGGACTGCGACGAGATCGGAAGAGCACACGTCTG	4007	0.12624563992550658	Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATCGAATCTTTGAACGCACATTGCGCCCCTTAGATCGGAAGAGCACACGTC	3833	0.12076354824918059	No Hit
GTCAAGGATGCTGGCATAATGGTTATATGCCAGATCGGAAGAGCACACGTC	3777	0.1189991969050757	No Hit
GAATACTGCCAGCTGGGACTGAGGACTGCGACGAGATCGGAAGAGCACACG	3641	0.11471434364082092	No Hit
ACGATCAACTTAGAACTGGTACGGACAAGAGATCGGAAGAGCACACGTCTG	3634	0.1144937997228078	Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TATGGAATCTAAACCATAGTTATGACGATTGCTCTAGATCGGAAGAGCACA	3630	0.11436777462680031	No Hit
AGATTAAGCCTTTGTTGTCTGATTTGTAGATCGGAAGAGCACACGTCTGAA	3469	0.1092952645124987	Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TGAGATTAAGCCTTTGTTGTCTGATTTGTAGATCGGAAGAGCACACGTCTG	3402	0.10718434415437318	Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCTGCTGAGATTAAGCCTTTGTTGTCTGATTTGTAGATCGGAAGAGCACAC	3226	0.10163923993004347	No Hit
AATACTGCCAGCTGGGACTGAGGACTGCGACGTAGATCGGAAGAGCACACG	3207	0.10104062072400788	No Hit
ATACTGCCAGCTGGGACTGAGGACTGCGACGAGATCGGAAGAGCACACGTC	3201	0.10085158307999663	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA Adapter	Nextera Transposase Sequence
1	0.0018903764401124019	0.0	0.0
2	0.0019848952621180217	0.0	0.0
3	0.002016401536119895	0.0	0.0
4	0.0020479078101217686	0.0	0.0
5	0.002079414084123642	0.0	0.0
6	0.002079414084123642	0.0	0.0
7	0.002173932906129262	0.0	0.0
8	0.0022999580021367556	0.0	0.0
9	0.0023629705501405024	0.0	0.0
10	0.0024574893721461223	0.0	0.0
11	0.002520501920149869	0.0	0.0
12	0.003056108578181716	0.0	0.0
13	0.003812259154226677	0.0	0.0
14	0.005041003840299738	0.0	0.0
15	0.0074984932124458606	0.0	0.0
16	0.013264141354788686	0.0	0.0
17	0.02952137873975534	0.0	0.0
18	0.060681083727608096	0.0	0.0
19	0.15063149600295656	0.0	0.0
20	0.2644951702457269	0.0	0.0
21	0.6582605827211402	0.0	0.0
22	1.001615956793556	0.0	0.0
23	1.8202749804582337	0.0	0.0
24	2.8367934048546757	0.0	0.0
25	4.248715567974629	0.0	0.0
26	6.197158071072483	0.0	0.0
27	11.054102258653277	0.0	0.0
28	17.048517456523705	0.0	0.0
29	28.585327339159683	0.0	0.0
30	42.792388462276435	0.0	0.0
31	59.28125997370486	0.0	0.0
32	66.17139854144855	0.0	0.0
33	72.36446079690079	0.0	0.0
34	79.62561724729053	0.0	0.0
35	85.96830279797767	0.0	0.0
36	93.56777361859955	0.0	0.0
37	94.83230943193873	0.0	0.0
38	95.18265919883956	0.0	0.0
39	95.408779727351	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGGAGGT	30	2.1667147E-6	45.000004	32
TCTAGTC	60	0.0	45.000004	2
GTCCTGT	25	3.8923652E-5	45.0	9
GTCGGTT	65	0.0	44.999996	6
TTGCTCC	760	0.0	44.703945	1
ATAGCCT	2000	0.0	44.6625	1
ATTCCAG	19725	0.0	44.46388	1
TTGCTCG	7595	0.0	44.17051	1
TCTCATA	630	0.0	42.857143	5
CCGATGT	190	0.0	42.63158	2
TGCTCGA	7925	0.0	42.359623	2
CTCATAA	640	0.0	42.187504	6
CAGTCTC	635	0.0	42.165356	2
TAGCCTG	33105	0.0	42.070686	1
TTTGATG	2210	0.0	42.04751	1
AGTCGGT	75	0.0	42.0	5
CGAGAGT	625	0.0	41.760002	22
ATGTAGT	200	0.0	41.625	5
GGTCGAG	650	0.0	41.53846	19
GTCTAGT	65	0.0	41.53846	1
>>END_MODULE