FastQCFastQC Report
Wed 16 Sep 2015
000000000-AHBE6 l01n01 150915_gms6.341000000347d1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AHBE6 l01n01 150915_gms6.341000000347d1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3173971
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAAAAAATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGTCT41224112.988177900806278Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GAAAAAATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGTCTG3071079.675797289893323Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAAAAATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGTCTGA2504797.891660005715238Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TAGCCTGTGGGAATACTGCCAGCTGGGACTGAGGAAGATCGGAAGAGCACA1663755.241856337061681No Hit
AAAAATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGTCTGAA1344024.234506238399784Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AAAATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGTCTGAAC1098773.4618148685038395Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTAGATCGGAAGAGCACACG1023733.225391788393782No Hit
TTGAAAAAATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGTC786312.477369831041304No Hit
ACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACAC672692.119395545832019No Hit
TAGCCTGTGGGAATACTGCCAGCTGGGACTGAGGAGATCGGAAGAGCACAC660672.0815250044817675No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATTAGATCGGAAGAGCACACGT509891.606473405081521No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAATAGATCGGAAGAGCACACG393161.2387006686576532No Hit
TTCCAGGGGGCATGCCTGTTTGAGCGTCATTTAGATCGGAAGAGCACACGT253950.800101828277574No Hit
AAATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGTCTGAACT236720.7458165181723463Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TTCCAGGGGGCATGCCTGTTTGAGCGTCATTAGATCGGAAGAGCACACGTC234170.7377824183018685No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCAGATCGGAAGAGCACACGTCTG186330.5870564034769064Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTGATGATTCATAATAACTTTTCGAATCGCAAGATCGGAAGAGCACACGTC184730.5820153996366066No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCAAGATCGGAAGAGCACACGT181960.5732881617380877No Hit
TTTGAAAAAATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGT179150.5644348987435612No Hit
CTTTGAAAAAATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACG172680.5440503394643492No Hit
TACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACA163170.5140878728885676No Hit
TAGCCTGTGGGAATACTGCCAGCTGGGACTAGATCGGAAGAGCACACGTCT155190.48894586623507275Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TAGCCTGTGGGAATACTGCCAGCTGGGACTGAGATCGGAAGAGCACACGTC141250.44502612027646127No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAAAGATCGGAAGAGCACACGT137950.43462904985584305No Hit
AATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGTCTGAACTC118950.3747671292522836Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGCCTGTGGGAATACTGCCAGCTGGGACTGAGGAAGATCGGAAGAGCACAC118680.3739164598542331No Hit
TCCGATATAGTGTAACGGCTATCACATCACGAGATCGGAAGAGCACACGTC96830.3050752511601398No Hit
GGAATACTGCCAGCTGGGACTGAGGACTGCGACGAGATCGGAAGAGCACAC95360.3004438288818644No Hit
ATAGCCTGTGGGAATACTGCCAGCTGGGACTGAGGAAGATCGGAAGAGCAC89020.2804688511646767No Hit
ATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGTCTGAACTCC82250.2591391036654084Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AATACTGCCAGCTGGGACTGAGGACTGCGACGAGATCGGAAGAGCACACGT78030.24584345603661786No Hit
GGGAATACTGCCAGCTGGGACTGAGGACTGCGACGAGATCGGAAGAGCACA75820.23888056948220385No Hit
TTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGTCTGAACTCCA75280.23717923068610267Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ATGCCTGTTTGAGCGTCATTTCCTTCTCAAACAGATCGGAAGAGCACACGT70930.22347400149528776No Hit
AGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGTCTGAACTCCAGT70330.22158362505517534Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TAGCCTGTGGGAATACTGCCAGCTGGGACTGAGAGATCGGAAGAGCACACG67280.211974211484604No Hit
TTACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCAC62490.19688270623770665No Hit
TTGCTCGAATATATTAGCATGGAATAATAGATCGGAAGAGCACACGTCTGA58780.18519387858301162Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATAGCCTGTGGGAATACTGCCAGCTGGGACTGAGGAGATCGGAAGAGCACA58550.18446923428096854No Hit
TTCCAGGGGGCATGCCTGTTTGAGCGTCAGATCGGAAGAGCACACGTCTGA52340.1649038381258052Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATTCCAGGGGGCATGCCTGTTTGAGCGTAGATCGGAAGAGCACACGTCTGA51290.16159567935560848Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTGCTCGAATATATTAGCATGGAATAGATCGGAAGAGCACACGTCTGAACT50500.1591066837094605Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGCCTGTGGGAATACTGCCAGCTGGGACTGAGGAGATCGGAAGAGCACACG48820.15381362967714576No Hit
TCAGTCTCATAATCTGAAGGTCGAGAGTAGATCGGAAGAGCACACGTCTGA45160.1422823333924601Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCTGAGATTAAGCCTTTGTTGTCTGATTTGTAGATCGGAAGAGCACACGTC44660.14070701969236643No Hit
TTGCTCCACACAATATAGATGGATACGAATAAGGCGTCAGATCGGAAGAGC44220.13932074363628402No Hit
GAGATTAAGCCTTTGTTGTCTGATTTGTAGATCGGAAGAGCACACGTCTGA41350.13027844299774635Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACTGCCAGCTGGGACTGAGGACTGCGACGAGATCGGAAGAGCACACGTCTG40070.12624563992550658Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATCGAATCTTTGAACGCACATTGCGCCCCTTAGATCGGAAGAGCACACGTC38330.12076354824918059No Hit
GTCAAGGATGCTGGCATAATGGTTATATGCCAGATCGGAAGAGCACACGTC37770.1189991969050757No Hit
GAATACTGCCAGCTGGGACTGAGGACTGCGACGAGATCGGAAGAGCACACG36410.11471434364082092No Hit
ACGATCAACTTAGAACTGGTACGGACAAGAGATCGGAAGAGCACACGTCTG36340.1144937997228078Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TATGGAATCTAAACCATAGTTATGACGATTGCTCTAGATCGGAAGAGCACA36300.11436777462680031No Hit
AGATTAAGCCTTTGTTGTCTGATTTGTAGATCGGAAGAGCACACGTCTGAA34690.1092952645124987Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TGAGATTAAGCCTTTGTTGTCTGATTTGTAGATCGGAAGAGCACACGTCTG34020.10718434415437318Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCTGCTGAGATTAAGCCTTTGTTGTCTGATTTGTAGATCGGAAGAGCACAC32260.10163923993004347No Hit
AATACTGCCAGCTGGGACTGAGGACTGCGACGTAGATCGGAAGAGCACACG32070.10104062072400788No Hit
ATACTGCCAGCTGGGACTGAGGACTGCGACGAGATCGGAAGAGCACACGTC32010.10085158307999663No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGAGGT302.1667147E-645.00000432
TCTAGTC600.045.0000042
GTCCTGT253.8923652E-545.09
GTCGGTT650.044.9999966
TTGCTCC7600.044.7039451
ATAGCCT20000.044.66251
ATTCCAG197250.044.463881
TTGCTCG75950.044.170511
TCTCATA6300.042.8571435
CCGATGT1900.042.631582
TGCTCGA79250.042.3596232
CTCATAA6400.042.1875046
CAGTCTC6350.042.1653562
TAGCCTG331050.042.0706861
TTTGATG22100.042.047511
AGTCGGT750.042.05
CGAGAGT6250.041.76000222
ATGTAGT2000.041.6255
GGTCGAG6500.041.5384619
GTCTAGT650.041.538461
Produced by FastQC (version 0.11.2)