##FastQC	0.11.2
>>Basic Statistics	pass
#Measure	Value
Filename	000000000-AHBE6 l01n01 150915_gms5.341000000347c4.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2159381
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.975873178471055	33.0	32.0	34.0	32.0	34.0
2	33.27534835214351	34.0	33.0	34.0	32.0	35.0
3	33.405881129823776	34.0	33.0	34.0	32.0	35.0
4	33.485235352168054	34.0	33.0	34.0	32.0	35.0
5	32.829910052927204	34.0	33.0	34.0	32.0	35.0
6	36.00441608034895	37.0	37.0	37.0	33.0	37.0
7	35.78511527145974	37.0	35.0	37.0	33.0	37.0
8	35.38020479016903	37.0	34.0	37.0	33.0	37.0
9	35.556931824444135	37.0	34.0	37.0	33.0	37.0
10	35.721640599782994	37.0	35.0	37.0	33.0	37.0
11	35.663839313210595	37.0	34.0	37.0	33.0	37.0
12	35.70435045969192	37.0	35.0	37.0	33.0	37.0
13	37.499560753753045	38.0	38.0	38.0	37.0	38.0
14	37.512072209582286	38.0	38.0	38.0	37.0	38.0
15	37.43451109368842	38.0	38.0	38.0	37.0	38.0
16	37.45808127421701	38.0	38.0	38.0	37.0	38.0
17	37.49929956779281	38.0	38.0	38.0	37.0	38.0
18	37.44364241419184	38.0	38.0	38.0	37.0	38.0
19	37.46968506252486	38.0	38.0	38.0	37.0	38.0
20	37.48021354267728	38.0	38.0	38.0	37.0	38.0
21	37.501890124994155	38.0	38.0	38.0	37.0	38.0
22	37.522256609648785	38.0	38.0	38.0	37.0	38.0
23	38.150333359421055	39.0	38.0	39.0	37.0	39.0
24	38.01710027086466	39.0	38.0	39.0	37.0	39.0
25	38.116067058106005	39.0	38.0	39.0	37.0	39.0
26	38.00644582868887	39.0	38.0	39.0	37.0	39.0
27	37.77782290387847	38.0	38.0	39.0	37.0	39.0
28	37.32511863353433	38.0	38.0	39.0	36.0	39.0
29	37.552542603644284	38.0	38.0	39.0	36.0	39.0
30	37.65146771227495	38.0	38.0	39.0	36.0	39.0
31	37.81649556053332	38.0	38.0	39.0	37.0	39.0
32	37.85073407610792	38.0	38.0	39.0	37.0	39.0
33	37.929592786080825	39.0	38.0	39.0	37.0	39.0
34	38.05300083681389	39.0	38.0	39.0	37.0	39.0
35	37.973880477785066	39.0	38.0	39.0	37.0	39.0
36	37.18210681672201	38.0	38.0	39.0	35.0	39.0
37	37.41596040717224	38.0	38.0	39.0	36.0	39.0
38	37.55501692383141	38.0	38.0	39.0	36.0	39.0
39	37.78145172158133	38.0	38.0	39.0	37.0	39.0
40	37.8533778893118	38.0	38.0	39.0	37.0	39.0
41	37.87728520349118	39.0	38.0	39.0	37.0	39.0
42	37.73796286991504	39.0	38.0	39.0	37.0	39.0
43	37.92501230676754	39.0	38.0	39.0	37.0	39.0
44	37.80912956073986	39.0	38.0	39.0	37.0	39.0
45	38.06516913874856	39.0	38.0	39.0	37.0	39.0
46	38.00913363598179	39.0	38.0	39.0	37.0	39.0
47	38.00200011021677	39.0	38.0	39.0	37.0	39.0
48	37.98205504262564	39.0	38.0	39.0	37.0	39.0
49	37.988846340687445	39.0	38.0	39.0	37.0	39.0
50	37.57760024747833	39.0	38.0	39.0	36.0	39.0
51	37.75189047231591	39.0	38.0	39.0	37.0	39.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	-0.0642979152354286
1101	2	0.12716066684570393
1101	3	0.18104325728266701
1101	4	0.14273052093607674
1101	5	0.004268958706582282
1101	6	0.07796980611031756
1101	7	0.10342380344475544
1101	8	0.16215515757139798
1101	9	0.09865558627831206
1101	10	0.08433114168271061
1101	11	0.10617225127132457
1101	12	0.10861531572822969
1101	13	0.05188589607261207
1101	14	0.04221989390372727
1101	15	0.10771147811230009
1101	16	0.0737310687823225
1101	17	0.06510277110646001
1101	18	0.10802023490477808
1101	19	0.07687497487551553
1101	20	0.08794843167677158
1101	21	0.07904158174777365
1101	22	0.05875803660088508
1101	23	0.08652456585239321
1101	24	0.04091328211718093
1101	25	0.05568530418218387
1101	26	0.10358315194199719
1101	27	0.11820645289976994
1101	28	0.4646679846894983
1101	29	0.2184781814505996
1101	30	0.23145317693156642
1101	31	0.12533612869103194
1101	32	0.1688663772983574
1101	33	0.10457551441408697
1101	34	0.07155129845050823
1101	35	0.124443732060044
1101	36	0.8419912319995788
1101	37	0.5211470771827109
1101	38	0.2577563856087721
1101	39	0.13345591371981413
1101	40	0.1016826084287814
1101	41	0.12535366236827628
1101	42	0.23074095864706834
1101	43	0.2201650556958299
1101	44	0.42133485373604884
1101	45	0.20864819781090915
1101	46	0.11952821072559061
1101	47	0.13869351428351706
1101	48	0.15820770403666273
1101	49	-0.008656779522624447
1101	50	0.5316030975829875
1101	51	0.1573718488911382
1102	1	-0.026618019303342066
1102	2	0.10579956473301166
1102	3	0.07835139189666762
1102	4	0.16624418587566936
1102	5	0.14502815152982862
1102	6	0.08771886925119077
1102	7	0.15756612402306303
1102	8	0.10301942639092232
1102	9	-4.050769571364299E-4
1102	10	-0.022867007842634735
1102	11	0.0507657305071092
1102	12	0.11382716112676405
1102	13	0.04019644940287748
1102	14	0.0854980023222538
1102	15	0.05766049907604298
1102	16	-0.021197051775473597
1102	17	-0.04254300681371603
1102	18	-0.006207440801937025
1102	19	0.040298814134231975
1102	20	0.09271302532041403
1102	21	0.15393378329966367
1102	22	0.07228054078888135
1102	23	0.06788865029847813
1102	24	-0.041304484618173376
1102	25	0.04877052218089517
1102	26	0.12954387939946344
1102	27	0.08623181707866934
1102	28	0.4291768417709818
1102	29	0.15420932262151155
1102	30	0.21458624097715528
1102	31	0.12043014768462257
1102	32	0.16701861444632726
1102	33	0.1609357366630988
1102	34	0.09690853407570899
1102	35	0.20266492314033258
1102	36	0.7861475796322281
1102	37	0.5196100586665295
1102	38	0.3148943202312182
1102	39	0.15256074601367686
1102	40	0.15589714562392487
1102	41	0.2057092556795297
1102	42	0.24809670775626103
1102	43	0.215457269182302
1102	44	0.42295396338606395
1102	45	0.1951259883846177
1102	46	0.18234499153545158
1102	47	0.06030064088783149
1102	48	0.06751488336073663
1102	49	0.1142873272067888
1102	50	0.4947821536243566
1102	51	0.13907928159237315
1103	1	0.15823296944373055
1103	2	0.09516409688176708
1103	3	0.0065340541212677294
1103	4	0.045650099109415976
1103	5	-0.08144617416260758
1103	6	0.07930878149529264
1103	7	-0.701839993531749
1103	8	-1.114303490209366
1103	9	-0.9737759224245579
1103	10	-0.27590383084829284
1103	11	-0.08614273598134758
1103	12	-0.03926545569102302
1103	13	0.07234586353244765
1103	14	0.14017806233600538
1103	15	0.1011702656736162
1103	16	0.0752512411877575
1103	17	0.08536036267332037
1103	18	0.08598738317900256
1103	19	0.19351220290938187
1103	20	0.1587644630478593
1103	21	0.1584084652390274
1103	22	0.05699995486079246
1103	23	0.0963092049573504
1103	24	-0.18124297183915417
1103	25	0.06494400128618594
1103	26	0.0361067934467485
1103	27	0.20090453771786088
1103	28	0.46610367692564836
1103	29	0.19329179560637044
1103	30	0.2292898727638928
1103	31	0.14000602772428294
1103	32	0.20933826140380063
1103	33	0.1242525695530432
1103	34	0.18453187648312763
1103	35	0.21178704958452244
1103	36	0.8667469807692072
1103	37	0.610640050268124
1103	38	0.3813496966619354
1103	39	0.1954464735903727
1103	40	0.17582254618172755
1103	41	0.23955296685409877
1103	42	0.3259025746232851
1103	43	0.13489797364087508
1103	44	0.37133082975027065
1103	45	0.13750507051976513
1103	46	0.08779567070575922
1103	47	0.1583124451834479
1103	48	0.17430505907786653
1103	49	0.17767611752594092
1103	50	0.5357021506991302
1103	51	0.33312613443599304
2112	1	-0.14790278913260124
2112	2	0.059325387126492046
2112	3	0.05885563052921583
2112	4	0.14264799198647182
2112	5	0.1512996749500175
2112	6	0.11370460683338734
2112	7	-0.5700412635982914
2112	8	-0.017128434876262588
2112	9	0.05842999151889927
2112	10	0.07042970281860761
2112	11	-0.02115163030901357
2112	12	-0.007887426249226337
2112	13	-0.018629229936436786
2112	14	0.07317903503994927
2112	15	0.045341162274944224
2112	16	-0.20784012298729238
2112	17	0.03549902729319854
2112	18	0.04983779434554947
2112	19	-0.029502262139651236
2112	20	0.029534347361256152
2112	21	0.13147325504566965
2112	22	0.15550517680866704
2112	23	0.13168642341024395
2112	24	-0.3566755595937039
2112	25	-0.03167229234972524
2112	26	0.0940044012708583
2112	27	0.06705468169252526
2112	28	0.4153789762795128
2112	29	0.23619986663548076
2112	30	0.19445910635524655
2112	31	0.19166384833643946
2112	32	0.1585071184645983
2112	33	0.09758165484928583
2112	34	0.09886369162592956
2112	35	0.10807556854018685
2112	36	0.707347595286393
2112	37	0.5505749780635654
2112	38	0.2850973825678622
2112	39	0.12934001681535534
2112	40	0.06325342506680443
2112	41	0.053024071754066426
2112	42	0.24255174227351262
2112	43	-0.07880942832239413
2112	44	0.23011738760926193
2112	45	0.13777974473634913
2112	46	0.020697683454699245
2112	47	0.09323155145437312
2112	48	0.08852560461271963
2112	49	0.1679244587398614
2112	50	0.43853506043558355
2112	51	0.16853810955601034
1113	1	0.007775958432695518
1113	2	0.006287820470291194
1113	3	0.10614896949532238
1113	4	0.15654319034150177
1113	5	0.09002097049197744
1113	6	0.08905830339039511
1113	7	0.1451350243027747
1113	8	0.14818417634408831
1113	9	0.17853469553065793
1113	10	0.12260759051913794
1113	11	0.09566224347084784
1113	12	0.09616278086661367
1113	13	0.12357681092390749
1113	14	0.07830611276493471
1113	15	0.040598941808696054
1113	16	0.12679978362266553
1113	17	0.09397067574204243
1113	18	0.13030939651152096
1113	19	0.09475013016948708
1113	20	0.027681444791930687
1113	21	0.11280723881660037
1113	22	-0.003444181793540224
1113	23	0.039130093864727655
1113	24	0.12020672161796853
1113	25	0.018925440769400836
1113	26	0.06433725111149613
1113	27	0.033237123694938475
1113	28	0.3929599261154735
1113	29	0.13251448339052274
1113	30	0.12556183625061834
1113	31	0.11119349614576635
1113	32	0.11662963529998649
1113	33	0.19263695527662605
1113	34	0.08461208139592458
1113	35	0.05972851729102757
1113	36	0.7878150590206374
1113	37	0.515747710291663
1113	38	0.3513126448675763
1113	39	0.13286207558547858
1113	40	0.12838603984452135
1113	41	0.0033073685199909164
1113	42	0.1546139532061659
1113	43	0.002548288087602657
1113	44	0.2550005106961848
1113	45	-0.0036350110868852425
1113	46	0.03874502947843439
1113	47	0.11531604741956869
1113	48	0.08277049875923836
1113	49	0.06932448174616468
1113	50	0.4096576075068654
1113	51	0.2763227867845188
2113	1	-0.30965585497732206
2113	2	0.01025529391884561
2113	3	0.0562336866273796
2113	4	0.12994823831176205
2113	5	0.12119317129785401
2113	6	0.16765784638727155
2113	7	0.12342772606119468
2113	8	0.10581114186189211
2113	9	0.12522380985924997
2113	10	0.09218718258474468
2113	11	0.05860209864095367
2113	12	0.09209894551415232
2113	13	0.12434592260149913
2113	14	0.07583352398446408
2113	15	0.07626963166124767
2113	16	0.09354107506353415
2113	17	0.10471656988482891
2113	18	0.12791528409216824
2113	19	0.03813728659621063
2113	20	0.06865955695472081
2113	21	0.09529859258183393
2113	22	0.09784749777194435
2113	23	0.08921744798920628
2113	24	0.15652962537401294
2113	25	0.06584146979619021
2113	26	0.09084515905505697
2113	27	-0.014774607354240743
2113	28	0.3786386402926283
2113	29	0.1472887084998007
2113	30	0.23136548986195749
2113	31	0.07499509110279234
2113	32	0.008025485734044935
2113	33	0.07912064889557513
2113	34	0.06194174752068449
2113	35	-0.03196534329055112
2113	36	0.7741066989145509
2113	37	0.5145433781831699
2113	38	0.2706932856894113
2113	39	0.10463188821633196
2113	40	0.09237845837462544
2113	41	0.058755118165329634
2113	42	0.2798431065142424
2113	43	0.11933014787334884
2113	44	0.47268321843335315
2113	45	0.16758622377632548
2113	46	0.12753546472353605
2113	47	0.084436999494514
2113	48	0.08456372028187076
2113	49	0.05617424058637255
2113	50	0.41972755148060514
2113	51	0.1102820085470455
1112	1	0.1881021114315331
1112	2	0.07173991217065634
1112	3	0.1615497536511441
1112	4	-0.009872332058584732
1112	5	0.0589302142020145
1112	6	-0.01483191946441309
1112	7	0.0069215737903505214
1112	8	-0.04080381049121229
1112	9	0.05026523379291348
1112	10	0.062070014707224175
1112	11	0.07152672743217181
1112	12	0.07012437879189548
1112	13	0.05363082286809373
1112	14	-0.035017538364044754
1112	15	0.15585553156473964
1112	16	0.09266299209998863
1112	17	0.08445599540154802
1112	18	0.14671510929227338
1112	19	0.004239214107805367
1112	20	-0.08374537418033157
1112	21	0.06261130659656544
1112	22	-0.04584983867179915
1112	23	0.01183415262558185
1112	24	0.07859425635022887
1112	25	0.034234834362152355
1112	26	-0.27504559208108503
1112	27	0.02495541677714641
1112	28	0.32374697445604994
1112	29	0.06534777629795485
1112	30	0.15690188562091834
1112	31	0.1035375068544937
1112	32	0.19066754545948328
1112	33	0.060491459776443435
1112	34	0.10276206190343373
1112	35	0.12445090806570391
1112	36	0.7932508756252545
1112	37	0.5077888269058519
1112	38	0.2748767147235398
1112	39	0.12773617424122818
1112	40	0.02389956824685413
1112	41	0.2066247042687408
1112	42	0.1552438234937128
1112	43	0.23179967571012838
1112	44	0.4389338918735106
1112	45	0.07894282145487352
1112	46	-0.009294053966236504
1112	47	0.08383619179475232
1112	48	0.08175361380602908
1112	49	0.0231958892107329
1112	50	0.34920732096324514
1112	51	0.11655912689793269
2114	1	0.1376746925930874
2114	2	0.1473594293634548
2114	3	-0.07291295792384744
2114	4	0.06316132784195361
2114	5	0.04905102272264372
2114	6	-0.001805249809542886
2114	7	0.06614896488125765
2114	8	0.024404242698686573
2114	9	0.07057936796294229
2114	10	0.0898957281391688
2114	11	0.0472398925727191
2114	12	0.05778659518001916
2114	13	3.00050846149702E-4
2114	14	0.019854022541807126
2114	15	0.03576978099378181
2114	16	0.08690457712485511
2114	17	0.024692863344597527
2114	18	0.07984887377968874
2114	19	-0.07568357302587003
2114	20	0.059187540805474725
2114	21	0.03463511612037706
2114	22	0.021616971001421348
2114	23	0.06462037917357577
2114	24	0.13565227005324232
2114	25	0.010402365471414043
2114	26	0.005178415944357084
2114	27	-0.0019183425876505567
2114	28	0.40317724275341504
2114	29	0.018509928653294594
2114	30	0.17546125468454932
2114	31	0.037748889749202874
2114	32	0.04098877659649247
2114	33	-0.022971539946084363
2114	34	0.0656510610710086
2114	35	0.08382157251256928
2114	36	0.767067603123067
2114	37	0.5346972706957374
2114	38	0.21884962885557968
2114	39	0.15284716440152835
2114	40	0.0823828450178965
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2108	8	0.024109479335976403
2108	9	-0.02408240637032577
2108	10	0.03160234684134622
2108	11	-0.057418955618359746
2108	12	-0.007848923226575266
2108	13	-0.37463944248489156
2108	14	-0.17764974672609668
2108	15	-0.17442021986587264
2108	16	-0.050765949183976034
2108	17	-0.02051933982555454
2108	18	-0.22936367986625328
2108	19	-0.09349352918333409
2108	20	-0.13235152732569588
2108	21	-0.19407719946654112
2108	22	-0.1367240221014825
2108	23	-0.057987788896099346
2108	24	-0.051458652227097446
2108	25	-0.18902918594603335
2108	26	0.021539022465852042
2108	27	-0.1837007930085548
2108	28	0.24200541373269857
2108	29	0.04100551464219393
2108	30	0.03972495795719766
2108	31	-0.0796320642062156
2108	32	-0.08645314692043371
2108	33	-0.2938786605560395
2108	34	-0.1814495783819794
2108	35	-0.2993945222613732
2108	36	-0.06080704226818057
2108	37	0.2410522894428908
2108	38	-0.1647705049864996
2108	39	-0.22338751881465413
2108	40	-0.14210177372396515
2108	41	-0.15944174420316415
2108	42	0.11300235374018541
2108	43	0.18788806318045914
2108	44	0.2539959870744255
2108	45	0.01130916886395994
2108	46	-0.10019491412902681
2108	47	-0.3808127875504539
2108	48	-0.32139716235908367
2108	49	-0.242511709889925
2108	50	0.2547102811344004
2108	51	-0.20315939455700516
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	2.0
17	1.0
18	2.0
19	2.0
20	4.0
21	13.0
22	52.0
23	192.0
24	438.0
25	1069.0
26	2014.0
27	3589.0
28	5817.0
29	9295.0
30	13665.0
31	18374.0
32	25538.0
33	35650.0
34	53563.0
35	91962.0
36	246356.0
37	1633712.0
38	18071.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	20.061952939291398	35.639055822015656	38.257167215975315	6.041824022717622
2	23.68581551842866	34.76065594723673	33.544937183387276	8.008591350947333
3	14.498321509728946	48.16148701873361	19.72148499963647	17.618706471900975
4	13.58722708035312	49.57235429968125	10.372370600648981	26.468048019316647
5	8.965485942499264	56.3047002821642	16.782170446067646	17.947643329268896
6	23.309040877918257	49.8762376810762	18.54012793481095	8.2745935061946
7	34.24578617668674	39.06318523688038	19.58376034613623	7.107268240296641
8	31.484161433299633	30.27710255855729	28.969598232085954	9.26913777605712
9	32.76096251657304	22.144725733902447	33.38336310266693	11.710948646857595
10	39.00886411429943	25.7748401046411	26.692001087348643	8.524294693710836
11	33.01793430617385	33.389707513403145	14.597377674435405	18.994980505987595
12	30.94451604418118	38.12189696954822	14.346333509464055	16.58725347680655
13	28.80598653039922	34.192437555021556	30.095661673414742	6.905914241164482
14	39.00891042386684	14.818413239720085	34.645437743501496	11.52723859291158
15	31.66810303508274	14.037865480894757	33.65348680941436	20.64054467460814
16	29.209713339146724	13.192067541577886	28.61259777686291	28.985621342412475
17	17.810196533173166	17.489039683131416	44.47584747666114	20.224916307034285
18	25.555054897676694	21.17704101314219	33.51270572446456	19.755198364716556
19	23.886845350588896	30.334202255183314	24.938350388375188	20.840602005852602
20	15.330272888387922	39.991321587065926	31.27988993142016	13.398515593125992
21	18.156638407024975	40.381433382992626	30.175962463317035	11.285965746665363
22	41.126785870580505	28.41888485635467	16.589568955177434	13.864760317887395
23	37.249100552426825	32.987786777784926	9.619979058813612	20.143133610974626
24	34.25143594391171	31.546957206718034	11.403777286175993	22.797829563194266
25	41.36444657056814	26.52329533324596	8.370685858586327	23.74157223759957
26	49.37326946935256	18.144366371659284	11.558266003081439	20.924098155906716
27	43.89044823493399	15.443731328561286	22.00417619678973	18.66164423971499
28	27.231322309495177	18.344886798577924	23.285098831563307	31.138692060363592
29	30.235099780909437	36.376443064007695	16.639027573179536	16.749429581903332
30	30.191198310997457	33.67506706783101	30.161421259147875	5.972313362023654
31	27.085030386022662	40.0236919746909	25.526342965877724	7.364934673408722
32	28.789870800937862	37.26692973588264	24.424545737875807	9.518653725303688
33	30.32619070002005	31.76734443805887	17.70882489009582	20.19763997182526
34	38.4206399889598	27.787314975912082	16.84311383678934	16.948931198338784
35	39.05883213754312	28.31167820778269	13.029520959941761	19.599968694732425
36	40.087228701187975	34.38828071563101	9.040368513013682	16.48412207016733
37	38.52650366007666	41.63846028097867	4.598678973279843	15.236357085664828
38	43.03279504635819	42.388304796606064	4.451136691487051	10.127763465548693
39	34.75820154016359	48.00634996788432	4.406447958928971	12.829000533023121
40	40.45548238129353	42.2672515873762	2.3374754154083974	14.939790615921877
41	34.58111375435831	36.8803837766471	4.432566554952554	24.105935914042036
42	29.992298718938436	40.58510286049567	3.8287361053931654	25.59386231517273
43	20.152071357486243	37.38927961300021	4.821659540396067	37.63698948911748
44	18.283526621749473	40.22560168863207	5.016067104415571	36.47480458520289
45	14.443398362771553	33.80292778347128	7.570919629282651	44.182754224474515
46	21.94513149833216	29.666279364317834	11.28545634142377	37.10313279592624
47	18.82215320038474	21.33912449910414	21.59544795476111	38.24327434575001
48	19.880187887176927	17.60129407455192	23.266806552433312	39.25171148583784
49	19.544026737291844	14.652115583123127	33.38239060175115	32.421467077833874
50	26.43822465789965	16.04482951364303	29.929132468980697	27.587813359476627
51	19.930341148690296	26.464019086951307	28.24489981156637	25.360739952792027
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2.0
1	1.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	1.0
10	2.0
11	2.0
12	2.0
13	3.5
14	5.0
15	6.5
16	8.0
17	14.5
18	21.0
19	23.0
20	25.0
21	30.5
22	36.0
23	40.0
24	44.0
25	70.5
26	136.5
27	176.0
28	197.0
29	218.0
30	346.0
31	474.0
32	673.0
33	872.0
34	1995.5
35	3119.0
36	12030.0
37	20941.0
38	24785.5
39	28630.0
40	62407.0
41	96184.0
42	325518.5
43	554853.0
44	448658.5
45	342464.0
46	224325.5
47	106187.0
48	112821.0
49	119455.0
50	128951.5
51	138448.0
52	192344.5
53	246241.0
54	261384.5
55	276528.0
56	200230.0
57	123932.0
58	98775.0
59	73618.0
60	47094.0
61	20570.0
62	11834.5
63	3099.0
64	2388.5
65	1678.0
66	1252.5
67	827.0
68	554.0
69	281.0
70	210.5
71	140.0
72	121.5
73	103.0
74	79.5
75	39.0
76	22.0
77	16.5
78	11.0
79	6.5
80	2.0
81	3.0
82	4.0
83	2.0
84	0.0
85	0.5
86	1.0
87	1.0
88	1.0
89	1.0
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	1.0
96	2.0
97	1.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2159381.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	11.421477086862437
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.54337931180034	8.74238452960748
2	10.844006609643303	2.477091460436516
3	4.067437922278799	1.39368447094628
4	2.198888040794894	1.0045819749805884
5	1.2932496165604819	0.7385410431569587
6	0.8914994182300734	0.6109344106959586
7	0.586347844602007	0.4687870930437098
8	0.4446404026204152	0.40627601363378885
9	0.34579830435290815	0.3554574668858361
>10	2.153670025514156	4.844250705385555
>50	0.28099189625591187	2.255478106178061
>100	0.263718104811107	6.163093090479676
>500	0.03422483990220359	2.7211130137396187
>1k	0.03870334346178757	8.822467427213242
>5k	0.004481439723901972	4.1400945806647655
>10k+	0.008962879447803943	54.85576461295197
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TGAAAAAATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGTCT	240475	11.136293224771359	Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GAAAAAATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGTCTG	190202	8.808172341981336	Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTCCAGGGGGCATGCCTGTTTGAGCGTCATTAGATCGGAAGAGCACACGTC	146286	6.774441379265632	No Hit
AAAAAATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGTCTGA	114061	5.282115569230257	Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATTAGATCGGAAGAGCACACGT	86246	3.9940149515069363	No Hit
AAAAATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGTCTGAA	56402	2.611952221493104	Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTGAAAAAATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGTC	51488	2.3843870072025273	No Hit
AAAATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGTCTGAAC	43147	1.9981189053714932	Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTAGATCGGAAGAGCACACG	28082	1.3004652722238457	No Hit
GGAATACTGCCAGCTGGGACTGAGGACTGCGACGAGATCGGAAGAGCACAC	26447	1.2247491294959065	No Hit
AGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGTCTGAACTCCAGT	26192	1.212940189804393	Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TAGCCTGTGGGAATACTGCCAGCTGGGACTGAGGAAGATCGGAAGAGCACA	20468	0.9478642259054794	No Hit
ACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACAC	19870	0.9201711045896949	No Hit
AATACTGCCAGCTGGGACTGAGGACTGCGACGAGATCGGAAGAGCACACGT	19791	0.9165126487636966	No Hit
TTCCAGGGGGCATGCCTGTTTGAGCGTCATTTAGATCGGAAGAGCACACGT	18893	0.8749266572226022	No Hit
TTCCAGGGGGCATGCCTGTTTGAGCGTCAGATCGGAAGAGCACACGTCTGA	16744	0.7754073968419654	Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATTCCAGGGGGCATGCCTGTTTGAGCGTCAGATCGGAAGAGCACACGTCTG	16723	0.7744348959261936	Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCCAGGGGGCATGCCTGTTTGAGCGTCATTAGATCGGAAGAGCACACGTCT	12190	0.5645136268217604	Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GAATACTGCCAGCTGGGACTGAGGACTGCGACGAGATCGGAAGAGCACACG	12189	0.5644673172543427	No Hit
AAATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGTCTGAACT	11170	0.5172778680557066	Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TTTGAAAAAATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGT	10918	0.5056078570664464	No Hit
ACTGCCAGCTGGGACTGAGGACTGCGACGAGATCGGAAGAGCACACGTCTG	10911	0.5052836900945225	Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGTCTGAACTCCA	9727	0.45045316227196586	Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ATTCCAGGGGGCATGCCTGTTTGAGCGTAGATCGGAAGAGCACACGTCTGA	9577	0.4435067271593109	Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTTTGAAAAAATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACG	8957	0.4147947953603371	No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAATAGATCGGAAGAGCACACG	8439	0.39080643943796856	No Hit
CCAGGGGGCATGCCTGTTTGAGCGTCATTAGATCGGAAGAGCACACGTCTG	7913	0.3664476069762585	Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGTCTGAACTCC	7801	0.3612609354254761	Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTCCAGGGGGCATGCCTGTTTGAGCGTAGATCGGAAGAGCACACGTCTGAA	7741	0.3584823613804141	Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGTCTGAACTC	7518	0.34815532784626707	Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGGGGGCATGCCTGTTTGAGCGTCATTAGATCGGAAGAGCACACGTCTGAA	7355	0.34060686835718196	Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CTATGTTCCTTGGAACAGGACGTCAGATCGGAAGAGCACACGTCTGAACTC	7026	0.3253710206767587	Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TAGCCTGTGGGAATACTGCCAGCTGGGACTGAGGAGATCGGAAGAGCACAC	6920	0.3204622065304826	No Hit
CTATGTTCCTTGGAACAGGACGTAGATCGGAAGAGCACACGTCTGAACTCC	4731	0.21909056345313774	Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGGAATACTGCCAGCTGGGACTGAGGACTGCGACGAGATCGGAAGAGCACA	4595	0.21279246228433055	No Hit
AGAGGTGCACAATCGACCGATCCTGAAGATCGGAAGAGCACACGTCTGAAC	4348	0.20135399913215873	Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGTCTGAACTCCAG	4088	0.18931351160355678	Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TTGCTCGAATATATTAGCATGGAATAGATCGGAAGAGCACACGTCTGAACT	4011	0.18574767491239388	Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGCCTGTGGGAATACTGCCAGCTGGGACTGAGGAAGATCGGAAGAGCACAC	3927	0.1818576712493071	No Hit
AGAGGTGCACAATCGACCGATCCTGAGATCGGAAGAGCACACGTCTGAACT	3805	0.17620790402434772	Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CAGGGGGCATGCCTGTTTGAGCGTCATTAGATCGGAAGAGCACACGTCTGA	3773	0.17472599786698134	Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AAGCCTTTGTTGTCTGATTTGTAGATCGGAAGAGCACACGTCTGAACTCCA	3759	0.17407766392313354	Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TTGATGATTCATAATAACTTTTCGAATCGCAAGATCGGAAGAGCACACGTC	3659	0.16944670718136354	No Hit
TTCCAGGGGGCATGCCTGTTTGAGCGTCATAGATCGGAAGAGCACACGTCT	3505	0.16231503379903778	Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTATGTTCCTTGGAACAGGACGTCATAGATCGGAAGAGCACACGTCTGAAC	3495	0.1618519381248608	Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TTGCTCGAATATATTAGCATGGAATAATAGAAAGATCGGAAGAGCACACGT	3381	0.156572647439243	No Hit
ATGCCTGTTTGAGCGTCATTTCCTTCTCAAACAGATCGGAAGAGCACACGT	3376	0.1563410996021545	No Hit
TAGCCTGTGGGAATACTGCCAGCTGGGACTAGATCGGAAGAGCACACGTCT	3093	0.14323549202294547	Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTACAACGGGGTATTGTAAGCAGTAGAGTAGATCGGAAGAGCACACGTCTG	3050	0.14124418062398436	Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGAAAAAATTAGAGTGTTCAAAGAAGGCGTAGATCGGAAGAGCACACGTCT	3027	0.14017906057337728	Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AATACTGCCAGCTGGGACTGAGGACTGCGACGTAGATCGGAAGAGCACACG	2800	0.12966678876955942	No Hit
TACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACA	2772	0.12837012088186384	No Hit
TGAAAAAATTAGAGTGTTCAAAGCAGGAGTAGATCGGAAGAGCACACGTCT	2643	0.12239618668498056	Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCAGGGGGCATGCCTGTTTGAGCGTCATTTAGATCGGAAGAGCACACGTCT	2587	0.11980285090958938	Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GAAAAAATTAGAGTGTTCAAAGAAGGCGTAGATCGGAAGAGCACACGTCTG	2516	0.11651487162293268	Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATCGAATCTTTGAACGCACATTGCGCCCCTAGATCGGAAGAGCACACGTCT	2482	0.1149403463307309	Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTGCCAGCTGGGACTGAGGACTGCGACGAGATCGGAAGAGCACACGTCTGA	2448	0.1133658210385291	Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATACTGCCAGCTGGGACTGAGGACTGCGACGAGATCGGAAGAGCACACGTC	2435	0.112763796662099	No Hit
GAGATTAAGCCTTTGTTGTCTGATTTGTAGATCGGAAGAGCACACGTCTGA	2424	0.1122543914205043	Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTGCTCGAATATATTAGCATGGAATAATAGATCGGAAGAGCACACGTCTGA	2371	0.10979998434736621	Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TGAAAAAATTAGAGTGTTAAAAGCAGGCGTAGATCGGAAGAGCACACGTCT	2355	0.10905903126868302	Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGAAAAAATTAGAGTGTTCAAAGCAGGCGTAGATAGGAAGAGCACACGTCT	2323	0.10757712511131662	Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
GCTGAGATTAAGCCTTTGTTGTCTGATTTGTAGATCGGAAGAGCACACGTC	2322	0.10753081554389893	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA Adapter	Nextera Transposase Sequence
1	0.0011577391854424948	0.0	0.0
2	0.0012966678876955942	0.0	0.0
3	0.0015282157247840933	0.0	0.0
4	0.0016208348596194928	0.0	0.0
5	0.0018523826967079917	0.0	0.0
6	0.0022228592360495902	0.0	0.0
7	0.003195360151821286	0.0	0.0
8	0.004214170635010681	0.0	0.0
9	0.0062054820339717725	0.0	0.0
10	0.007872626461008964	0.0	0.0
11	0.011716320556678048	0.0	0.0
12	0.032416697192389855	0.0	0.0
13	0.050106951945951175	0.0	0.0
14	0.08817341636330041	0.0	0.0
15	0.14096632321947816	0.0	0.0
16	0.20816150554256058	0.0	0.0
17	0.2906388451134839	0.0	0.0
18	0.3846935765388322	0.0	0.0
19	0.6169360571385967	0.0	0.0
20	1.1108739032157826	0.0	0.0
21	3.1291837799813926	0.0	0.0
22	4.380375672472806	0.0	0.0
23	6.243455879254286	0.0	0.0
24	8.237638471395275	0.0	0.0
25	10.555293391948897	0.0	0.0
26	12.75615558347508	0.0	0.0
27	16.749383272335916	0.0	0.0
28	22.79208717683447	0.0	0.0
29	37.90563128970756	0.0	0.0
30	51.81508034015303	0.0	0.0
31	66.3746694075756	0.0	0.0
32	78.15197966454275	0.0	0.0
33	86.26583266223052	0.0	0.0
34	89.96758793376435	0.0	0.0
35	93.25612293523005	0.0	0.0
36	94.87149326589426	0.0	0.0
37	94.95262762801006	0.0	0.0
38	94.96119489798234	0.0	0.0
39	94.96777085655565	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTCCCGT	20	7.0346636E-4	45.000004	3
GAGCGGC	35	1.2125929E-7	45.000004	23
TTATAGT	135	0.0	45.0	2
CCGAACG	30	2.1663927E-6	44.999996	2
TAGCCTG	4015	0.0	44.495644	1
ATTCCAG	16475	0.0	44.481033	1
TTGCTCG	2240	0.0	44.29688	1
TTTTCCT	245	0.0	43.163265	4
TGCATGC	190	0.0	42.63158	5
TGCTCGA	2340	0.0	42.596153	2
CTATGTT	2045	0.0	42.139362	1
CTGTGAG	75	0.0	42.0	24
TCAGTCT	140	0.0	41.785717	1
GCTCGAA	2390	0.0	41.705017	3
CTCATAA	130	0.0	41.53846	6
CTCGAAT	2400	0.0	41.53125	4
TGTAACG	250	0.0	41.4	11
ATCGGGC	55	6.184564E-11	40.909092	20
AGTTGCA	210	0.0	40.714287	3
AGGATGC	315	0.0	40.714287	5
>>END_MODULE