FastQCFastQC Report
Wed 16 Sep 2015
000000000-AHBE6 l01n01 150915_gms5.341000000347c4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AHBE6 l01n01 150915_gms5.341000000347c4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2159381
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAAAAAATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGTCT24047511.136293224771359Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GAAAAAATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGTCTG1902028.808172341981336Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTCCAGGGGGCATGCCTGTTTGAGCGTCATTAGATCGGAAGAGCACACGTC1462866.774441379265632No Hit
AAAAAATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGTCTGA1140615.282115569230257Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATTAGATCGGAAGAGCACACGT862463.9940149515069363No Hit
AAAAATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGTCTGAA564022.611952221493104Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTGAAAAAATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGTC514882.3843870072025273No Hit
AAAATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGTCTGAAC431471.9981189053714932Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTAGATCGGAAGAGCACACG280821.3004652722238457No Hit
GGAATACTGCCAGCTGGGACTGAGGACTGCGACGAGATCGGAAGAGCACAC264471.2247491294959065No Hit
AGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGTCTGAACTCCAGT261921.212940189804393Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TAGCCTGTGGGAATACTGCCAGCTGGGACTGAGGAAGATCGGAAGAGCACA204680.9478642259054794No Hit
ACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACAC198700.9201711045896949No Hit
AATACTGCCAGCTGGGACTGAGGACTGCGACGAGATCGGAAGAGCACACGT197910.9165126487636966No Hit
TTCCAGGGGGCATGCCTGTTTGAGCGTCATTTAGATCGGAAGAGCACACGT188930.8749266572226022No Hit
TTCCAGGGGGCATGCCTGTTTGAGCGTCAGATCGGAAGAGCACACGTCTGA167440.7754073968419654Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATTCCAGGGGGCATGCCTGTTTGAGCGTCAGATCGGAAGAGCACACGTCTG167230.7744348959261936Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCCAGGGGGCATGCCTGTTTGAGCGTCATTAGATCGGAAGAGCACACGTCT121900.5645136268217604Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GAATACTGCCAGCTGGGACTGAGGACTGCGACGAGATCGGAAGAGCACACG121890.5644673172543427No Hit
AAATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGTCTGAACT111700.5172778680557066Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TTTGAAAAAATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGT109180.5056078570664464No Hit
ACTGCCAGCTGGGACTGAGGACTGCGACGAGATCGGAAGAGCACACGTCTG109110.5052836900945225Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGTCTGAACTCCA97270.45045316227196586Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ATTCCAGGGGGCATGCCTGTTTGAGCGTAGATCGGAAGAGCACACGTCTGA95770.4435067271593109Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTTTGAAAAAATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACG89570.4147947953603371No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAATAGATCGGAAGAGCACACG84390.39080643943796856No Hit
CCAGGGGGCATGCCTGTTTGAGCGTCATTAGATCGGAAGAGCACACGTCTG79130.3664476069762585Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGTCTGAACTCC78010.3612609354254761Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTCCAGGGGGCATGCCTGTTTGAGCGTAGATCGGAAGAGCACACGTCTGAA77410.3584823613804141Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGTCTGAACTC75180.34815532784626707Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGGGGGCATGCCTGTTTGAGCGTCATTAGATCGGAAGAGCACACGTCTGAA73550.34060686835718196Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CTATGTTCCTTGGAACAGGACGTCAGATCGGAAGAGCACACGTCTGAACTC70260.3253710206767587Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TAGCCTGTGGGAATACTGCCAGCTGGGACTGAGGAGATCGGAAGAGCACAC69200.3204622065304826No Hit
CTATGTTCCTTGGAACAGGACGTAGATCGGAAGAGCACACGTCTGAACTCC47310.21909056345313774Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGGAATACTGCCAGCTGGGACTGAGGACTGCGACGAGATCGGAAGAGCACA45950.21279246228433055No Hit
AGAGGTGCACAATCGACCGATCCTGAAGATCGGAAGAGCACACGTCTGAAC43480.20135399913215873Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGTCTGAACTCCAG40880.18931351160355678Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TTGCTCGAATATATTAGCATGGAATAGATCGGAAGAGCACACGTCTGAACT40110.18574767491239388Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGCCTGTGGGAATACTGCCAGCTGGGACTGAGGAAGATCGGAAGAGCACAC39270.1818576712493071No Hit
AGAGGTGCACAATCGACCGATCCTGAGATCGGAAGAGCACACGTCTGAACT38050.17620790402434772Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CAGGGGGCATGCCTGTTTGAGCGTCATTAGATCGGAAGAGCACACGTCTGA37730.17472599786698134Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AAGCCTTTGTTGTCTGATTTGTAGATCGGAAGAGCACACGTCTGAACTCCA37590.17407766392313354Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TTGATGATTCATAATAACTTTTCGAATCGCAAGATCGGAAGAGCACACGTC36590.16944670718136354No Hit
TTCCAGGGGGCATGCCTGTTTGAGCGTCATAGATCGGAAGAGCACACGTCT35050.16231503379903778Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTATGTTCCTTGGAACAGGACGTCATAGATCGGAAGAGCACACGTCTGAAC34950.1618519381248608Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TTGCTCGAATATATTAGCATGGAATAATAGAAAGATCGGAAGAGCACACGT33810.156572647439243No Hit
ATGCCTGTTTGAGCGTCATTTCCTTCTCAAACAGATCGGAAGAGCACACGT33760.1563410996021545No Hit
TAGCCTGTGGGAATACTGCCAGCTGGGACTAGATCGGAAGAGCACACGTCT30930.14323549202294547Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTACAACGGGGTATTGTAAGCAGTAGAGTAGATCGGAAGAGCACACGTCTG30500.14124418062398436Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGAAAAAATTAGAGTGTTCAAAGAAGGCGTAGATCGGAAGAGCACACGTCT30270.14017906057337728Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AATACTGCCAGCTGGGACTGAGGACTGCGACGTAGATCGGAAGAGCACACG28000.12966678876955942No Hit
TACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACA27720.12837012088186384No Hit
TGAAAAAATTAGAGTGTTCAAAGCAGGAGTAGATCGGAAGAGCACACGTCT26430.12239618668498056Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCAGGGGGCATGCCTGTTTGAGCGTCATTTAGATCGGAAGAGCACACGTCT25870.11980285090958938Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GAAAAAATTAGAGTGTTCAAAGAAGGCGTAGATCGGAAGAGCACACGTCTG25160.11651487162293268Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATCGAATCTTTGAACGCACATTGCGCCCCTAGATCGGAAGAGCACACGTCT24820.1149403463307309Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTGCCAGCTGGGACTGAGGACTGCGACGAGATCGGAAGAGCACACGTCTGA24480.1133658210385291Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATACTGCCAGCTGGGACTGAGGACTGCGACGAGATCGGAAGAGCACACGTC24350.112763796662099No Hit
GAGATTAAGCCTTTGTTGTCTGATTTGTAGATCGGAAGAGCACACGTCTGA24240.1122543914205043Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTGCTCGAATATATTAGCATGGAATAATAGATCGGAAGAGCACACGTCTGA23710.10979998434736621Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TGAAAAAATTAGAGTGTTAAAAGCAGGCGTAGATCGGAAGAGCACACGTCT23550.10905903126868302Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGAAAAAATTAGAGTGTTCAAAGCAGGCGTAGATAGGAAGAGCACACGTCT23230.10757712511131662Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
GCTGAGATTAAGCCTTTGTTGTCTGATTTGTAGATCGGAAGAGCACACGTC23220.10753081554389893No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCCCGT207.0346636E-445.0000043
GAGCGGC351.2125929E-745.00000423
TTATAGT1350.045.02
CCGAACG302.1663927E-644.9999962
TAGCCTG40150.044.4956441
ATTCCAG164750.044.4810331
TTGCTCG22400.044.296881
TTTTCCT2450.043.1632654
TGCATGC1900.042.631585
TGCTCGA23400.042.5961532
CTATGTT20450.042.1393621
CTGTGAG750.042.024
TCAGTCT1400.041.7857171
GCTCGAA23900.041.7050173
CTCATAA1300.041.538466
CTCGAAT24000.041.531254
TGTAACG2500.041.411
ATCGGGC556.184564E-1140.90909220
AGTTGCA2100.040.7142873
AGGATGC3150.040.7142875
Produced by FastQC (version 0.11.2)