FastQCFastQC Report
Tue 29 Mar 2016
000000000-AHBBJ_l01n03.3330000005057b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AHBBJ_l01n03.3330000005057b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10428462
Sequences flagged as poor quality0
Sequence length251
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCNNNTTGNNNCCNANGCTNTCGCANATGNGNNNCAGNATATTCCCAAGG187300.17960462434441435No Hit
CCNNNTTGNNNCCNANGCTNTCGCANCTGNGNNNCAGNCTTCGTCCAGGG181210.17376483703924894No Hit
CCNNNTTGNNNCCNACGCTNTCGCANATGNGNNTCAGTATATTCCCAAGG139370.13364386809867074No Hit
CCNNNTTGNNNCCNACGCTNTCGCANCTGNGNNTCAGTCTTCGTCCAGGG127420.12218484374781247No Hit
CCNNNTTGNNNCCNANGCTNTCGAGNCTCNGNNNCAGNTACAGTCCAGAG120380.11543408797960811No Hit
CCNNNTCGNNNCCNANGCTNTCGAGNCTCNGNNNCAGNTACAGACCAGAG116110.11133952446679098No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAGTTA73900.03783.60630-34
TGAGTTA5750.03537.256830-34
TGAGATA1700.02498.030530-34
GCCTTCG529600.01699.615855-59
GCTTTCG43200.01556.132155-59
GCACATG31550.01233.235420-24
TCAGTAT142850.01182.863430-34
TCAGTCA25950.01162.758330-34
TCGCACA41500.01156.72520-24
CGCACAT36800.01139.685220-24
TCGTGCA15000.01132.728120-24
TCAGATA33850.01129.09530-34
GTGCCTC32100.01115.687720-24
CGTGCAT13850.01085.40520-24
CGAGCCT57400.01084.999820-24
GTGCATG10650.01079.409920-24
CGTGCCT34750.01059.688820-24
TCAGTCT78650.0991.7930-34
TCAGCTA8950.0973.945430-34
TCAATAA16900.0965.4486730-34
Produced by FastQC (version 0.11.2)