FastQCFastQC Report
Tue 29 Mar 2016
000000000-AHBBJ_l01n02.3320000005057e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AHBBJ_l01n02.3320000005057e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10428462
Sequences flagged as poor quality0
Sequence length12
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTCGAATCGT6268266.010723345398391No Hit
TATGCACCAGTG6131855.879917863247716No Hit
AGTTCCCGAGTA4925224.72286325634595No Hit
GACACATTTCTG4912644.710800116066971No Hit
TAACACCACATC4886844.686060130439177No Hit
GTACGTGGGATC4844914.645852859223153No Hit
CGGACTACAACT4811434.613748412757317No Hit
TCAACAGCATCG4112373.9434098719446835No Hit
CGCCAAATAACC2972022.8499120963378877No Hit
ATGATGACCCGT2591552.48507402146165No Hit
GAATGATGAGTG2452692.351919199590505No Hit
ATTAGTTCGCGT2387472.289378817317453No Hit
CCATAGGGTTCA1890051.8123957300702636No Hit
ACGGCATGGCAT1740171.6686736740278671No Hit
AGCCTAAGCACG1706831.6367034755460586No Hit
TTATGCAGTCGT1650841.5830138710770583No Hit
TACTTCGCTCGC1513061.4508946765112631No Hit
CGTCCGAAATAC1391561.3343866046594406No Hit
CTTACACCAAGT1380871.3241358121648235No Hit
AAGATGGATCAG1278341.2258183421486313No Hit
TCTGTTGCTCTC1185561.1368502853057336No Hit
CTAGATTTGCCA1109261.0636851340111322No Hit
GCGGCAATTACG941160.9024916617618207No Hit
TGGCACCGATTA590300.5660470355072493No Hit
CTATTTGCGACA561760.5386796250492163No Hit
GGTGTCTATTGT535400.5134026474853147No Hit
GTCCGAAACACT430130.41245775263888385No Hit
GAACTAGTCACC378630.36307367280045705No Hit
GTGGGATGTTTC368660.35351329850940627No Hit
ATACCTTCGGTA324450.31111970298208885No Hit
CCATACATAGCT305370.29282362058758044No Hit
ACGATGCGACCA302810.29036880030823337No Hit
ATCGATCTGTGG244360.234320266977048No Hit
CTCGAGAGTACG240230.23035995144825766No Hit
GCAGGATAGATA208990.2004034727268508No Hit
CCATGCGATAAC201560.19327874043171467No Hit
ATGTCGAGAGAA199130.1909485789946782No Hit
AAGGAGCGCCTT196360.188292386739291No Hit
TGAGTCACTGGT185960.17831967935444365No Hit
CGATCCGTATTA178780.17143467560221248No Hit
GGTCAGCTTAAC175810.16858670051250127No Hit
CGAGAAGAGAAC157210.15075089692037044No Hit
CTACCGGATCAA156040.1496289673395751No Hit
TAAACCGCGTGT154000.14767278242947043No Hit
TTGATGCTATGC149900.14374123432582867No Hit
AGGCTACACGAC146300.1402891433079969No Hit
ACCACATACATC144780.13883159376713458No Hit
TAGCGGATCACG136720.13110274554387788No Hit
TGGCATACGGCA134990.1294438240269754No Hit
AATGTCCGTGAC132720.12726708885739813No Hit
CCAAGTCTTACA118860.1139765384387458No Hit
CAGCTAGAACGC114300.10960388981615889No Hit
GTACATACCGGT104650.10035036806002649No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTTCC1303.6452548E-96.00002436
TGAGATC704.6965867E-46.00002346
GAGCGCC21450.05.99999574
ACGTGGT3950.05.99999573
AGCTGTT2100.05.99999571
ACGTCGG600.00339629245.99999573
ATCGGCG4450.05.99999571
GCTAACG11650.05.99999573
GGGCTAC550.0091600885.9999952
CGAAACG704.6968757E-45.9999955
CATGATG1303.6452548E-95.9999952
CACGCGG550.0091600885.9999952
GCTGATG5600.05.9999951
AATGCTG3450.05.9999952
CCACTCG806.538448E-55.9999955
TACTGGG909.150766E-65.9999952
CGGGCTA550.0091600885.9999951
CGCAGTC650.0012618515.9999954
AACTGGC550.0091600885.9999955
CGGTGAC1900.05.9999954