FastQCFastQC Report
Wed 5 Aug 2015
000000000-AH0J7 l01n01 150804_gms8.3410000000eea7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AH0J7 l01n01 150804_gms8.3410000000eea7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1811091
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAAAAAATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGTCT20602611.375795031834404Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GAAAAAATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGTCTG1174146.483053584828151Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTGAAAAAATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGTC1084825.989870194264121No Hit
AAAAAATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGTCTGA883374.877557229316473Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACAC757004.179801014968326No Hit
TAGCCTGTGGGAATACTGCCAGCTGGGACTGAGGAAGATCGGAAGAGCACA537752.969204750065016No Hit
AAAATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGTCTGAAC535792.9583825440024825Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AAAAATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGTCTGAA523142.888535142629498Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGTCTGAACTCCAGT372182.055004414466197Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TAGCCTGTGGGAATACTGCCAGCTGGGACTGAGGAGATCGGAAGAGCACAC355571.963291739619931No Hit
TTTGAAAAAATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGT351771.9423099115395086No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATTAGATCGGAAGAGCACACGT272251.5032375512881462No Hit
TTCCAGGGGGCATGCCTGTTTGAGCGTCATTAGATCGGAAGAGCACACGTC230271.2714435663365342No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTAGATCGGAAGAGCACACG224691.2406334082605457No Hit
CTTTGAAAAAATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACG215391.189283144800565No Hit
AAATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGTCTGAACT151230.8350215422637515Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGTCTGAACTCCA126670.6994126744597593Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGTCTGAACTCC115190.6360254675220627Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGTCTGAACTC101580.5608773937919188Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGCCTGTGGGAATACTGCCAGCTGGGACTGAGGAAGATCGGAAGAGCACAC92220.5091958383096156No Hit
TTCCAGGGGGCATGCCTGTTTGAGCGTCATTTAGATCGGAAGAGCACACGT85970.4746862526510264No Hit
AGCCTGTGGGAATACTGCCAGCTGGGACTGAGGAGATCGGAAGAGCACACG78220.43189436643437573No Hit
TAGCCTGTGGGAATACTGCCAGCTGGGACTAGATCGGAAGAGCACACGTCT73760.40726832610840646Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATTCCAGGGGGCATGCCTGTTTGAGCGTCAGATCGGAAGAGCACACGTCTG73540.40605358869322417Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTTGATGATTCATAATAACTTTTCGAATCGCAAGATCGGAAGAGCACACGT65350.3608322276462088No Hit
AATACTGCCAGCTGGGACTGAGGACTGCGACGAGATCGGAAGAGCACACGT63750.35199777371761No Hit
GGAATACTGCCAGCTGGGACTGAGGACTGCGACGAGATCGGAAGAGCACAC61940.3420037977108826No Hit
TAGCCTGTGGGAATACTGCCAGCTGGGACTGAGAGATCGGAAGAGCACACG61930.3419485823738288No Hit
TAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGTCTGAACTCCAG54690.30197267834691904Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TAGCCTGTGGGAATACTGCCAGCTGGGACTGAGATCGGAAGAGCACACGTC53130.29335908576653524No Hit
TACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACA48840.2696717061704796No Hit
GAAAAAATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGTCTA47950.26475754117269645Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
AATACTGCCAGCTGGGACTGAGGACTGCGACGTAGATCGGAAGAGCACACG44420.24526652719272526No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTAGATCGGAAGAGCACACGTCTGA43150.23825417938689994Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTGATGATTCATAATAACTTTTCGAATCGCAAGATCGGAAGAGCACACGTC41690.2301927401770535No Hit
TTCCAGGGGGCATGCCTGTTTGAGCGTCAGATCGGAAGAGCACACGTCTGA40040.22108220956318594Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TAGCCTGTGGGAATACTGCCAGCTGGGACTGAAGATCGGAAGAGCACACGT39980.22075091754086348No Hit
AATACTGCCAGCTGGGACTGAGGAAGATCGGAAGAGCACACGTCTGAACTC37890.20921091209663126Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TTGCTCGAATATATTAGCATGGAATAATAGAAAGATCGGAAGAGCACACGT37170.2052354078287618No Hit
ACTGCCAGCTGGGACTGAGGACTGCGACGAGATCGGAAGAGCACACGTCTG34470.19032726682425125Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTGCTCGAATATATTAGCATGGAATAGATCGGAAGAGCACACGTCTGAACT32720.18066458283984627Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AAGCCTTTGTTGTCTGATTTGTAGATCGGAAGAGCACACGTCTGAACTCCA31850.17586084851617065Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GCCAAGGACGTTTTCATTAATCAAGAACGAAGATCGGAAGAGCACACGTCT31270.17265835896705356Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTGCTCGAATATATTAGCATGGAATAATAGAATAGATCGGAAGAGCACACG30360.16763376329516297No Hit
TTACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCAC29880.16498342711658331No Hit
GAATACTGCCAGCTGGGACTGAGGACTGCGACGAGATCGGAAGAGCACACG28650.15819194065897296No Hit
GCCAAGGACGTTTTCATTAATCAAGAACAGATCGGAAGAGCACACGTCTGA28390.15675634189557563Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATCGAATCTTTGAACGCACATTGCGCCCCTTAGATCGGAAGAGCACACGTC27190.1501305014491265No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATTAGATCGGAAGAGCACACGTCT26920.14863968734867547Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCCTGTGGGAATACTGCCAGCTGGGACTGAGAGATCGGAAGAGCACACGT26780.14786667262992306No Hit
AGGGGGCATGCCTGTTTGAGCGTCATTAGATCGGAAGAGCACACGTCTGAA26160.14444332173259103Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GAATACTGCCAGCTGGGACTGAGGACTGCGACGTAGATCGGAAGAGCACAC25390.14019174077945284No Hit
ATACTGCCAGCTGGGACTGAGGAAGATCGGAAGAGCACACGTCTGAACTCC24360.13450456106291733Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CTATGTTCCTTGGAACAGGACGTCAGATCGGAAGAGCACACGTCTGAACTC23300.1286517353352206Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ATAGATGGATACGAATAAGGCGTCAGATCGGAAGAGCACACGTCTGAACTC23300.1286517353352206Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGTCTGAACTCCAGTC22870.12627747584190965Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GGAATACTGCCAGCTGGGACTGAGGACTGCGACGTAGATCGGAAGAGCACA21540.11893383601376188No Hit
AGCCTGTGGGAATACTGCCAGCTGGGACTGAAGATCGGAAGAGCACACGTC21250.11733259123920332No Hit
ACTGCCAGCTGGGACTGAGGACTGCGACGTAGATCGGAAGAGCACACGTCT20660.11407488635303252Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTACGATCTGCTGAGATTAAGCCTTTGTTAGATCGGAAGAGCACACGTCTG20010.11048588944453923Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TAAGCCTTTGTTGTCTGATTTGTAGATCGGAAGAGCACACGTCTGAACTCC19950.11015459742221678Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGATTAAGCCTTTGTTGTCTGATTTGTAGATCGGAAGAGCACACGTCTGAA19750.10905029068114193Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCTGAGATTAAGCCTTTGTTGTCTGATTTGTAGATCGGAAGAGCACACGTC19750.10905029068114193No Hit
GAGATTAAGCCTTTGTTGTCTGATTTGTAGATCGGAAGAGCACACGTCTGA19340.10678646186193846Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCCAAGGACGTTTTCATTAATCAGATCGGAAGAGCACACGTCTGAACTCCA18850.10408091034630508Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AAAAAAATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGTCTG18680.10314224961639143Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGATGGATACGAATAAGGCGTCAGATCGGAAGAGCACACGTCTGAACTCCA18500.10214837354942408Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TAGCCTGTGGGAATACTGCCAGCTGGGACTGAGGAAGATCGGAAGAGCACC18350.10132014349361794No Hit
AAAAAATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGTCTGG18150.10021583675254309Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGGACC253.891698E-545.00000410
TGGACCC253.891698E-545.00000411
GTGGGAC207.0343626E-445.0000047
GGGTACA253.891698E-545.0000041
GTCCTAT453.8562575E-1045.02
GGTCCTA453.8562575E-1045.01
ATGTAGT453.8562575E-1045.05
GTGTAGT453.8562575E-1045.010
CTCGCTA351.2124474E-745.017
TCACTTT453.8562575E-1045.022
CATGTGG302.166198E-644.9999964
ATTCCAG73300.044.570261
TTGCTCG14450.044.377161
TAGCCTG130400.044.1200141
CGATATA3250.042.9230773
ATAGCCT2350.042.127661
GCACACA4450.041.96629345
GGCATGT700.041.78571310
ATATAGT3450.041.0869565
CCGATAT3400.041.029412
Produced by FastQC (version 0.11.2)