##FastQC	0.11.2
>>Basic Statistics	pass
#Measure	Value
Filename	000000000-AH0J7 l01n01 150804_gms8.3410000000eea7.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1811091
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.36371060316682	33.0	32.0	34.0	32.0	34.0
2	32.66179391317167	33.0	32.0	34.0	32.0	34.0
3	32.75257179236162	33.0	32.0	34.0	32.0	34.0
4	32.820650094335406	33.0	32.0	34.0	32.0	34.0
5	32.606419003793846	33.0	32.0	34.0	32.0	34.0
6	35.61363288757992	37.0	37.0	37.0	33.0	37.0
7	35.44190987642256	37.0	35.0	37.0	33.0	37.0
8	35.03907368541945	37.0	34.0	37.0	33.0	37.0
9	35.31108652188101	37.0	35.0	37.0	33.0	37.0
10	35.475559759283215	37.0	35.0	37.0	33.0	37.0
11	35.213425498773944	37.0	34.0	37.0	32.0	37.0
12	35.14577125058873	37.0	34.0	37.0	33.0	37.0
13	36.8327698608187	38.0	38.0	38.0	35.0	38.0
14	36.89231794537105	38.0	38.0	38.0	36.0	38.0
15	36.75852290138927	38.0	38.0	38.0	34.0	38.0
16	36.82789489871023	38.0	38.0	38.0	35.0	38.0
17	36.768823322516646	38.0	38.0	38.0	34.0	38.0
18	36.823498653574006	38.0	38.0	38.0	35.0	38.0
19	36.80728466984817	38.0	38.0	38.0	35.0	38.0
20	36.83539976732257	38.0	38.0	38.0	35.0	38.0
21	36.8746517982807	38.0	38.0	38.0	36.0	38.0
22	36.90905702695226	38.0	38.0	38.0	36.0	38.0
23	37.366270938346005	39.0	38.0	39.0	35.0	39.0
24	37.33494782978878	39.0	38.0	39.0	35.0	39.0
25	37.334195796898115	39.0	38.0	39.0	35.0	39.0
26	37.225704837581326	38.0	38.0	39.0	35.0	39.0
27	36.94423140526898	38.0	37.0	39.0	34.0	39.0
28	36.78497380860487	38.0	37.0	39.0	34.0	39.0
29	36.79252064087338	38.0	37.0	39.0	34.0	39.0
30	36.820313280779374	38.0	37.0	39.0	34.0	39.0
31	36.85350156342227	38.0	37.0	39.0	34.0	39.0
32	36.92934369393918	38.0	37.0	39.0	34.0	39.0
33	36.966206557263	38.0	37.0	39.0	34.0	39.0
34	36.8795057785611	38.0	37.0	39.0	34.0	39.0
35	37.12433334382425	38.0	38.0	39.0	35.0	39.0
36	37.21527686902535	38.0	38.0	39.0	35.0	39.0
37	37.162548430752516	38.0	38.0	39.0	35.0	39.0
38	37.1343157246102	38.0	38.0	39.0	35.0	39.0
39	37.16928635833318	38.0	38.0	39.0	35.0	39.0
40	37.22624042635075	38.0	38.0	39.0	35.0	39.0
41	37.15375097109974	38.0	38.0	39.0	35.0	39.0
42	37.210361599720834	38.0	38.0	39.0	35.0	39.0
43	37.12729619881055	39.0	38.0	39.0	34.0	39.0
44	37.28023053507527	39.0	38.0	39.0	35.0	39.0
45	37.27287750863982	39.0	38.0	39.0	36.0	39.0
46	37.3429142986189	39.0	38.0	39.0	36.0	39.0
47	37.268534822380545	39.0	38.0	39.0	35.0	39.0
48	37.28981867835465	39.0	38.0	39.0	36.0	39.0
49	37.3456540836435	39.0	38.0	39.0	36.0	39.0
50	37.368824095531366	39.0	38.0	39.0	36.0	39.0
51	36.62300016951109	39.0	37.0	39.0	33.0	39.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	-0.13829654258451285
1101	2	0.15538246638539732
1101	3	0.09588382816772878
1101	4	0.059528514751136186
1101	5	0.1602917981604719
1101	6	0.12769356267970977
1101	7	0.10806421278352474
1101	8	0.29060050393955095
1101	9	0.02066449561010586
1101	10	-0.02298660556834875
1101	11	0.0015421813854885613
1101	12	0.06114167726743602
1101	13	0.16500416632933224
1101	14	0.020592557668564382
1101	15	-0.005178379879268391
1101	16	-0.0234247474324647
1101	17	0.08356939392923834
1101	18	0.0449730444382368
1101	19	0.1526856983308562
1101	20	0.14400725822262928
1101	21	0.043538114323894206
1101	22	0.025276830618231827
1101	23	-0.012540929148073587
1101	24	0.012285605435749858
1101	25	0.08363240363517832
1101	26	-0.18140625690422496
1101	27	0.00595974427032786
1101	28	-0.13052405679506052
1101	29	0.07291528167613848
1101	30	-0.05328787901232346
1101	31	0.023849478788434908
1101	32	-0.050364518816891746
1101	33	-0.0336850502757855
1101	34	0.1816072863723548
1101	35	-0.10731544127568071
1101	36	-0.048739394471745356
1101	37	0.05247901263616228
1101	38	-7.132445424460343E-4
1101	39	0.060148178287647625
1101	40	0.01209638972225946
1101	41	0.058884147973202516
1101	42	-0.09603886957761176
1101	43	0.007793432306137049
1101	44	-0.04561089561963172
1101	45	0.24735502306128154
1101	46	0.2059261854271881
1101	47	0.08728463137076403
1101	48	-0.023095038390913203
1101	49	0.042240290258050095
1101	50	0.036642494706001116
1101	51	0.5147548193265976
1102	1	0.34654692536024356
1102	2	0.0801245358095386
1102	3	0.051352670128359534
1102	4	0.12457030414157799
1102	5	0.16636363318119862
1102	6	-0.03088152226018792
1102	7	0.03808256610039962
1102	8	0.12720689467005997
1102	9	-0.15137775407129084
1102	10	-0.31672936613622227
1102	11	-0.03977361590572315
1102	12	0.033602196839062515
1102	13	0.12663939814589753
1102	14	-0.15336421647683807
1102	15	0.10405838912592458
1102	16	-0.16238186319133519
1102	17	-0.008634351439901877
1102	18	0.035076950275119145
1102	19	0.24563419331941816
1102	20	-0.0115880092607199
1102	21	-0.07745790099637162
1102	22	-0.07434879924167603
1102	23	0.17218037940944697
1102	24	0.016190744947685687
1102	25	-0.09989562786543615
1102	26	-0.2698513358946144
1102	27	0.12516720897004063
1102	28	-0.25787673806617306
1102	29	-0.24757406366911994
1102	30	-0.2918903049419441
1102	31	0.026013345097425145
1102	32	-0.08927286562387593
1102	33	-0.11970691964275204
1102	34	-0.01915106822361423
1102	35	0.11834188172703364
1102	36	-0.04812223183601816
1102	37	-0.009291502083875969
1102	38	-0.06614280802744332
1102	39	0.06726776029869086
1102	40	-0.05601572670060051
1102	41	0.1132077869376289
1102	42	-0.11809337540215381
1102	43	0.16039811151967598
1102	44	-0.080957056528284
1102	45	0.08959578999877493
1102	46	0.24010932186269685
1102	47	0.08655462337132747
1102	48	0.06177894520354954
1102	49	-0.011941417183386704
1102	50	-0.17252081685647624
1102	51	0.47230007057802936
1103	1	0.14377639081578764
1103	2	0.18913313867594184
1103	3	0.027297451563967456
1103	4	0.10867304333486061
1103	5	0.16789893515564813
1103	6	0.2408318716753115
1103	7	0.19558505437747442
1103	8	0.26845686414734615
1103	9	-0.10625661919313245
1103	10	0.03394998084591805
1103	11	-0.04366639050837051
1103	12	-0.042778246524022734
1103	13	0.16352798793663226
1103	14	0.0864809486761331
1103	15	-0.06326601322549408
1103	16	-0.2855525278285711
1103	17	-0.03458797597548369
1103	18	-0.113422550560621
1103	19	0.11797320042827408
1103	20	-0.04912676116321535
1103	21	-0.29606675073024036
1103	22	0.13406949998262263
1103	23	0.020312227075159228
1103	24	-0.1239812037433694
1103	25	-0.07886615020331789
1103	26	-0.05379563107917562
1103	27	0.15429232901452394
1103	28	-0.36590099438888046
1103	29	-0.38856526590999607
1103	30	0.10840613820054301
1103	31	-0.14108478051995377
1103	32	-0.2497335487588188
1103	33	-0.28682664691767457
1103	34	0.17271739392722907
1103	35	0.009724321671725988
1103	36	-0.10386572404748762
1103	37	-0.1401501941243879
1103	38	-0.01853086875360077
1103	39	-0.11002043802675132
1103	40	-0.19345236236239316
1103	41	-0.08781897329129151
1103	42	0.09292906776443033
1103	43	0.06630191996734425
1103	44	-0.25485920132140194
1103	45	0.03774270852986916
1103	46	0.10340078025296151
1103	47	0.0037042446746582414
1103	48	-0.17426480602411942
1103	49	-0.13850623283109087
1103	50	0.03160395803929106
1103	51	0.11589698847667051
2112	1	0.3147770371336591
2112	2	0.2227457125881429
2112	3	0.11726802561402394
2112	4	0.10670215951956408
2112	5	0.12930532724918464
2112	6	0.1461787946352615
2112	7	0.06765455802736398
2112	8	0.025004979953109796
2112	9	0.09316179208118314
2112	10	-0.003616593527780765
2112	11	-0.09295798352528095
2112	12	-0.24045103282662694
2112	13	0.018253908738643077
2112	14	-0.036998170293415455
2112	15	-0.1720883783155287
2112	16	-0.4447032058731679
2112	17	0.06308259094208779
2112	18	-0.155580056447171
2112	19	-0.01733940994026284
2112	20	-0.3027255289526565
2112	21	0.0951129437890117
2112	22	0.042557414482423894
2112	23	-0.07520226594602519
2112	24	-0.45840692887750123
2112	25	-0.007896632695377548
2112	26	-0.06221256769821082
2112	27	0.17967662651837202
2112	28	-0.9073594439185015
2112	29	0.004731706956157211
2112	30	0.028360209662395164
2112	31	-0.46641497146371336
2112	32	-0.3762008487416111
2112	33	0.09783916499866052
2112	34	0.07866327623276703
2112	35	-0.20193613258921062
2112	36	-0.27631391370210423
2112	37	0.0820646967209413
2112	38	-0.08756360458304613
2112	39	-0.20487580614621237
2112	40	-0.20728477322793282
2112	41	0.07094649540425735
2112	42	-0.1732298465453681
2112	43	-0.1384558473872275
2112	44	-0.5249777831388585
2112	45	0.040835717414580586
2112	46	-0.07528632792353562
2112	47	-0.35229432742943345
2112	48	-0.42708596520667896
2112	49	0.052815816889072664
2112	50	-0.10596236418830784
2112	51	-0.06918978760592864
1113	1	0.21265858477350008
1113	2	0.11498350399073587
1113	3	0.13649487679785466
1113	4	0.1435155881181842
1113	5	0.12810274476741768
1113	6	0.163818456059019
1113	7	0.12291302163514217
1113	8	0.05821960260539072
1113	9	0.17309304472770748
1113	10	0.15631174477962873
1113	11	0.09370266089444357
1113	12	0.20519027874585305
1113	13	0.22356445657789692
1113	14	0.1548972059149918
1113	15	0.06438913978375638
1113	16	0.08241552529472074
1113	17	0.19920814314720303
1113	18	0.01950837227550295
1113	19	0.22408575396306674
1113	20	0.11185932830441914
1113	21	0.1979761689709818
1113	22	0.12244698107470242
1113	23	0.01525900191234797
1113	24	0.21373102626751717
1113	25	0.201671100362951
1113	26	0.09424578120291471
1113	27	0.421069340641111
1113	28	0.20918911389878048
1113	29	0.39489222111003386
1113	30	0.23830066075315415
1113	31	-0.1640496846029862
1113	32	0.11472728818556277
1113	33	0.2254658470014661
1113	34	0.17875498063919082
1113	35	-0.10129801416357509
1113	36	0.057755619422444227
1113	37	0.21644338954158826
1113	38	0.17979016223711142
1113	39	-0.3781042311321201
1113	40	0.09952166282371877
1113	41	0.06818128772000875
1113	42	0.08921776400853076
1113	43	0.12759258340672375
1113	44	0.26905068590603776
1113	45	0.34996225136679726
1113	46	0.17085756467157154
1113	47	-0.1299341834044796
1113	48	-0.0077374998110926185
1113	49	0.31078073958514807
1113	50	0.173402537844062
1113	51	0.08761683759281169
2113	1	0.27603920022841066
2113	2	0.05319339118143063
2113	3	-0.04022255889012172
2113	4	5.722248552544329E-4
2113	5	0.06325478484340152
2113	6	0.1450859715456474
2113	7	0.10141078525403913
2113	8	-0.22440440046813848
2113	9	-0.019851231086967402
2113	10	0.04564487665768269
2113	11	-0.039114645811480386
2113	12	0.04805458162434206
2113	13	0.10330933228519967
2113	14	0.11143282644025021
2113	15	0.20398063030897617
2113	16	0.030181959298616334
2113	17	0.09634039726182664
2113	18	0.02272735617891186
2113	19	-0.07258421160638306
2113	20	0.12673104119986078
2113	21	0.2308292661013951
2113	22	0.07278516500378629
2113	23	0.21546871453213612
2113	24	0.2205904380623238
2113	25	0.23339747445891135
2113	26	0.12992529554917098
2113	27	0.10176521144857276
2113	28	0.1443836106260079
2113	29	0.3404918167406876
2113	30	0.2253121690230273
2113	31	0.2897673789333197
2113	32	0.1883273212601111
2113	33	0.2193851540934304
2113	34	0.20413483574727564
2113	35	0.09685644797480109
2113	36	0.1513418427087032
2113	37	0.2841984924123153
2113	38	0.17720805230807457
2113	39	0.08579137333610021
2113	40	0.2138386652892592
2113	41	0.09000809721207759
2113	42	0.08745203690546077
2113	43	0.20052093483045041
2113	44	0.3023294145960804
2113	45	0.3582086691897217
2113	46	0.13688808030494215
2113	47	0.21552653492236118
2113	48	0.12606008594977425
2113	49	0.34649387553265143
2113	50	0.21133540143674878
2113	51	0.4372072761419403
1112	1	0.23769918235110765
1112	2	0.10493865951466574
1112	3	0.08442984376788587
1112	4	0.10064196962893845
1112	5	0.09311338616863907
1112	6	-0.12622680027300248
1112	7	-0.10162537519703818
1112	8	-0.5035050139171915
1112	9	-0.17884249766949267
1112	10	-0.04626370171564531
1112	11	-0.15850455027376142
1112	12	0.0771762276263317
1112	13	-0.03306571659386748
1112	14	0.21885911763318688
1112	15	0.00402501423249646
1112	16	-0.1614843464736353
1112	17	0.0743388854511764
1112	18	0.02198253343863854
1112	19	-0.09309729580289172
1112	20	-0.0767077946016883
1112	21	0.14914908493353352
1112	22	0.002848942719204217
1112	23	-0.09666036293941715
1112	24	0.157065064570709
1112	25	0.21522013625404668
1112	26	-0.03382824277900909
1112	27	-0.02332913057303898
1112	28	0.10038526361173439
1112	29	0.23837690890127305
1112	30	0.09164100279463838
1112	31	0.1242012656667768
1112	32	0.14323416105607123
1112	33	0.19408047192806066
1112	34	0.22179158477565153
1112	35	0.10537549659899526
1112	36	0.08137877864839282
1112	37	0.2750493684211861
1112	38	0.23167513028558062
1112	39	-0.21046822278140098
1112	40	-0.1384779821213158
1112	41	0.08250460303155904
1112	42	0.17997524809961618
1112	43	-0.4525749814539992
1112	44	-0.1457848240013888
1112	45	0.04320506162584081
1112	46	0.053510875363890875
1112	47	-0.06271319311735368
1112	48	-0.293866025607862
1112	49	0.12798389416747114
1112	50	0.12980143731032
1112	51	-0.18981986859022726
2114	1	0.34108470048364836
2114	2	-0.10822056000707647
2114	3	0.013320765170760751
2114	4	0.03603670673270898
2114	5	0.08461791802433538
2114	6	-0.1287303590991371
2114	7	-0.07464479489969023
2114	8	-0.6806709189731635
2114	9	-0.1439590655045322
2114	10	0.009966842604193005
2114	11	-0.05365893001507516
2114	12	-0.049981361497351884
2114	13	-0.22685058427450855
2114	14	0.10488175976566083
2114	15	0.10556894988071264
2114	16	-0.004338884690568534
2114	17	0.19454829367352744
2114	18	0.09055846604898221
2114	19	0.08947898535389953
2114	20	0.06672304815074881
2114	21	0.00832659290981752
2114	22	0.14462896741817843
2114	23	-0.00801330085473495
2114	24	0.20710296766363712
2114	25	0.1544751247584628
2114	26	0.06509924204895867
2114	27	0.2361942852636929
2114	28	0.13015746527141658
2114	29	0.10284125445679848
2114	30	0.23072170837178874
2114	31	0.17012039347996222
2114	32	0.18501322053455738
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2108	2	-0.28653951829855373
2108	3	-0.05689044021735867
2108	4	-0.07781731122070568
2108	5	-0.22906957137596606
2108	6	0.041999507325222396
2108	7	-0.2869752247956754
2108	8	-0.0035696529562869728
2108	9	0.03620497994062788
2108	10	0.013372983477417222
2108	11	-0.21708846840166984
2108	12	0.1011434868678478
2108	13	-0.31422902302131916
2108	14	0.1432851819432699
2108	15	-0.09070854600745548
2108	16	0.09937267659570637
2108	17	-0.4944028453520417
2108	18	0.02103719928736325
2108	19	-0.08012348574862926
2108	20	-0.1450821680630341
2108	21	-0.36648039881003314
2108	22	-0.0607040661525744
2108	23	-0.0145540625769911
2108	24	-0.021359018670786156
2108	25	-0.6610336133999937
2108	26	-0.00579542388749843
2108	27	-0.009022388041842078
2108	28	-0.003378795025405168
2108	29	-1.0247337990887075
2108	30	-0.8384983976627041
2108	31	-0.30902056768321273
2108	32	-0.18015674442928997
2108	33	-0.3950312848185291
2108	34	-1.6235075670563575
2108	35	-0.5293990230881249
2108	36	-0.14853714058810397
2108	37	-0.23893578309508
2108	38	-0.24842637848395555
2108	39	-0.22049982419424907
2108	40	-0.1621426646053976
2108	41	-1.1022135768916357
2108	42	-0.686177967560468
2108	43	-0.11526172340202834
2108	44	-0.19215960718931058
2108	45	-0.508968173641108
2108	46	-0.18950284764565595
2108	47	-0.08793237371892104
2108	48	-0.1433295829197121
2108	49	-0.5930355701098335
2108	50	-0.23905722881734448
2108	51	0.3467715837130996
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	1.0
15	0.0
16	1.0
17	3.0
18	5.0
19	5.0
20	9.0
21	28.0
22	149.0
23	415.0
24	1229.0
25	2646.0
26	5117.0
27	8920.0
28	13545.0
29	19640.0
30	27100.0
31	36025.0
32	47138.0
33	62278.0
34	86234.0
35	130698.0
36	257073.0
37	1102314.0
38	10518.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	14.799863728548152	38.945917129509226	41.03581763699339	5.2184015049492265
2	24.596665766656674	37.96993083174727	27.40469694786181	10.028706453734241
3	23.192705391391154	45.49467696543134	21.21710063160824	10.095517011569271
4	14.491099563743623	50.144526144738165	14.373766972504418	20.990607319013787
5	9.201580704669174	51.46671260582709	21.459109453914795	17.872597235588934
6	20.12703944749325	49.24059586183135	23.808245968866277	6.8241187218091195
7	25.739181520972714	47.62438773093125	21.408476989836515	5.227953758259525
8	20.720990828180362	41.20494221438901	31.19655500469054	6.877511952740088
9	26.126848402427044	32.72679285579797	31.347348090184312	9.799010651590672
10	33.43553692221981	29.682274385991647	30.53899555571752	6.3431931360710205
11	31.68819236581707	37.74702651606132	20.871066114292436	9.693715003829183
12	29.90965114397896	41.68934636636149	16.857242402507662	11.543760087151888
13	28.515850390731334	37.36951925662487	28.294160812460557	5.820469540183238
14	31.47787714697936	21.383574872825275	37.9903052911201	9.148242689075259
15	28.94697174244696	25.59120441766869	31.539663109142502	13.922160730741856
16	31.825623339743835	15.900029319343975	29.596690613558348	22.677656727353842
17	22.602508653623698	21.910494834329143	39.55383799047094	15.933158521576221
18	26.529478640222937	21.535251403711904	33.33405113271503	18.601218823350123
19	21.03809250888001	26.342022570925483	24.32136209610671	28.2985228240878
20	18.35672531087615	37.18587304558413	21.47087032070724	22.986531322832484
21	18.574052877519684	47.902010445637465	21.43216436943257	12.091772307410285
22	35.04208236913551	32.92954357345931	16.64228909535744	15.386084962047738
23	33.593894508889946	30.583664763393998	9.771016475704423	26.051424252011635
24	29.660740404540686	30.775538059655755	16.654491684846317	22.909229850957242
25	41.49703134740331	29.961111838113048	8.245361497572459	20.296495316911187
26	47.23075759307511	24.620021854230405	10.95367378005854	17.195546772635943
27	53.30345079291985	18.654335977595828	12.77092095317132	15.271292276313005
28	30.830919042720655	23.87417308130845	17.356168188125277	27.938739687845615
29	32.296002796104666	29.529824840386265	15.047614945908293	23.12655741760077
30	36.15599657885772	27.195982973798667	29.128574986016716	7.519445461326901
31	35.08929148231646	37.082289073271305	19.05006429826	8.778355146152236
32	33.52310844678705	37.598331613375585	16.999808402780424	11.878751537056944
33	37.64647938728645	31.72734003978817	15.83062364066742	14.79555693225796
34	34.264043054711216	30.004455877700238	21.113240582610153	14.618260484978391
35	32.190596717669074	34.17221994919085	11.8869234069409	21.75025992619918
36	43.47655639611704	32.949034587439286	8.184017258105749	15.390391758337929
37	39.07495537220383	39.018746159083115	6.287977799017278	15.618320669695779
38	36.47353998225379	41.925999300973835	9.60128452960122	11.999176187171159
39	31.245972731353643	41.57863961556873	8.173912851424916	19.001474801652705
40	41.992312920775376	37.605896114551946	2.838565262595861	17.563225702076814
41	38.08737385366058	36.561387583506296	5.97540377595604	19.375834786877082
42	33.295676473462684	40.347779321966705	4.277587376890504	22.07895682768011
43	20.784598896466274	40.831465674557485	5.405912789583737	32.9780226393925
44	23.149361351803968	40.32536189512289	5.401937285315868	31.123339467757283
45	17.481396572563167	35.421356519357666	8.364626625608542	38.73262028247062
46	21.66881730404491	32.003858447753316	11.259953254695649	35.067370993506124
47	18.829755103415565	21.940918485045753	16.657859820406596	42.571466591132086
48	21.16321046264379	21.884709271925043	19.632420458165825	37.319659807265346
49	16.97496150110624	20.600952685425526	28.10101756344656	34.32306825002167
50	20.632591073557318	20.796911916629256	25.880974506526726	32.689522503286696
51	18.51922404782532	22.279112424499928	28.604139714680265	30.59752381299449
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	6.0
1	5.0
2	4.0
3	3.5
4	3.0
5	2.0
6	1.0
7	2.5
8	4.0
9	5.5
10	7.0
11	8.0
12	9.0
13	10.0
14	11.0
15	17.5
16	24.0
17	30.0
18	36.0
19	55.5
20	75.0
21	93.5
22	112.0
23	117.5
24	123.0
25	153.5
26	253.0
27	322.0
28	355.5
29	389.0
30	559.0
31	729.0
32	1004.5
33	1280.0
34	2375.0
35	3470.0
36	12547.0
37	21624.0
38	27420.5
39	33217.0
40	97688.5
41	162160.0
42	356893.5
43	551627.0
44	414158.0
45	276689.0
46	179372.0
47	82055.0
48	85019.5
49	87984.0
50	102154.5
51	116325.0
52	124836.5
53	133348.0
54	141109.5
55	148871.0
56	139611.0
57	130351.0
58	86606.5
59	42862.0
60	27024.0
61	11186.0
62	7026.5
63	2867.0
64	2212.5
65	1558.0
66	1282.0
67	1006.0
68	714.5
69	423.0
70	247.5
71	72.0
72	54.0
73	36.0
74	29.5
75	16.5
76	10.0
77	7.5
78	5.0
79	4.0
80	3.0
81	1.5
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1811091.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	9.276733956530062
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.24844810115763	7.166133015892592
2	9.237444016312146	1.7138662115533665
3	4.187992553470681	1.1655267819142954
4	2.177954339054442	0.8081721189151332
5	1.454646773014954	0.6747185556992352
6	0.78984700392738	0.4396320313077996
7	0.5806910756824937	0.37708416340264306
8	0.39621962735934024	0.2940499257094464
9	0.3560022280234706	0.2972284161575119
>10	2.6716627677901927	5.280242018460408
>50	0.4141927978214971	2.732285870242725
>100	0.37803440025483576	7.136804522687361
>500	0.04059656649437671	2.6214913594158697
>1k	0.0483575470320826	9.271401000885549
>5k	0.006567074288265284	4.2340095726279205
>10k+	0.01134312831609458	55.78735443512806
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TGAAAAAATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGTCT	206026	11.375795031834404	Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GAAAAAATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGTCTG	117414	6.483053584828151	Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTGAAAAAATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGTC	108482	5.989870194264121	No Hit
AAAAAATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGTCTGA	88337	4.877557229316473	Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACAC	75700	4.179801014968326	No Hit
TAGCCTGTGGGAATACTGCCAGCTGGGACTGAGGAAGATCGGAAGAGCACA	53775	2.969204750065016	No Hit
AAAATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGTCTGAAC	53579	2.9583825440024825	Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AAAAATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGTCTGAA	52314	2.888535142629498	Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGTCTGAACTCCAGT	37218	2.055004414466197	Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TAGCCTGTGGGAATACTGCCAGCTGGGACTGAGGAGATCGGAAGAGCACAC	35557	1.963291739619931	No Hit
TTTGAAAAAATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGT	35177	1.9423099115395086	No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATTAGATCGGAAGAGCACACGT	27225	1.5032375512881462	No Hit
TTCCAGGGGGCATGCCTGTTTGAGCGTCATTAGATCGGAAGAGCACACGTC	23027	1.2714435663365342	No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTAGATCGGAAGAGCACACG	22469	1.2406334082605457	No Hit
CTTTGAAAAAATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACG	21539	1.189283144800565	No Hit
AAATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGTCTGAACT	15123	0.8350215422637515	Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGTCTGAACTCCA	12667	0.6994126744597593	Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGTCTGAACTCC	11519	0.6360254675220627	Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGTCTGAACTC	10158	0.5608773937919188	Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGCCTGTGGGAATACTGCCAGCTGGGACTGAGGAAGATCGGAAGAGCACAC	9222	0.5091958383096156	No Hit
TTCCAGGGGGCATGCCTGTTTGAGCGTCATTTAGATCGGAAGAGCACACGT	8597	0.4746862526510264	No Hit
AGCCTGTGGGAATACTGCCAGCTGGGACTGAGGAGATCGGAAGAGCACACG	7822	0.43189436643437573	No Hit
TAGCCTGTGGGAATACTGCCAGCTGGGACTAGATCGGAAGAGCACACGTCT	7376	0.40726832610840646	Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATTCCAGGGGGCATGCCTGTTTGAGCGTCAGATCGGAAGAGCACACGTCTG	7354	0.40605358869322417	Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTTGATGATTCATAATAACTTTTCGAATCGCAAGATCGGAAGAGCACACGT	6535	0.3608322276462088	No Hit
AATACTGCCAGCTGGGACTGAGGACTGCGACGAGATCGGAAGAGCACACGT	6375	0.35199777371761	No Hit
GGAATACTGCCAGCTGGGACTGAGGACTGCGACGAGATCGGAAGAGCACAC	6194	0.3420037977108826	No Hit
TAGCCTGTGGGAATACTGCCAGCTGGGACTGAGAGATCGGAAGAGCACACG	6193	0.3419485823738288	No Hit
TAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGTCTGAACTCCAG	5469	0.30197267834691904	Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TAGCCTGTGGGAATACTGCCAGCTGGGACTGAGATCGGAAGAGCACACGTC	5313	0.29335908576653524	No Hit
TACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACA	4884	0.2696717061704796	No Hit
GAAAAAATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGTCTA	4795	0.26475754117269645	Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
AATACTGCCAGCTGGGACTGAGGACTGCGACGTAGATCGGAAGAGCACACG	4442	0.24526652719272526	No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTAGATCGGAAGAGCACACGTCTGA	4315	0.23825417938689994	Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTGATGATTCATAATAACTTTTCGAATCGCAAGATCGGAAGAGCACACGTC	4169	0.2301927401770535	No Hit
TTCCAGGGGGCATGCCTGTTTGAGCGTCAGATCGGAAGAGCACACGTCTGA	4004	0.22108220956318594	Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TAGCCTGTGGGAATACTGCCAGCTGGGACTGAAGATCGGAAGAGCACACGT	3998	0.22075091754086348	No Hit
AATACTGCCAGCTGGGACTGAGGAAGATCGGAAGAGCACACGTCTGAACTC	3789	0.20921091209663126	Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TTGCTCGAATATATTAGCATGGAATAATAGAAAGATCGGAAGAGCACACGT	3717	0.2052354078287618	No Hit
ACTGCCAGCTGGGACTGAGGACTGCGACGAGATCGGAAGAGCACACGTCTG	3447	0.19032726682425125	Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTGCTCGAATATATTAGCATGGAATAGATCGGAAGAGCACACGTCTGAACT	3272	0.18066458283984627	Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AAGCCTTTGTTGTCTGATTTGTAGATCGGAAGAGCACACGTCTGAACTCCA	3185	0.17586084851617065	Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GCCAAGGACGTTTTCATTAATCAAGAACGAAGATCGGAAGAGCACACGTCT	3127	0.17265835896705356	Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTGCTCGAATATATTAGCATGGAATAATAGAATAGATCGGAAGAGCACACG	3036	0.16763376329516297	No Hit
TTACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCAC	2988	0.16498342711658331	No Hit
GAATACTGCCAGCTGGGACTGAGGACTGCGACGAGATCGGAAGAGCACACG	2865	0.15819194065897296	No Hit
GCCAAGGACGTTTTCATTAATCAAGAACAGATCGGAAGAGCACACGTCTGA	2839	0.15675634189557563	Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATCGAATCTTTGAACGCACATTGCGCCCCTTAGATCGGAAGAGCACACGTC	2719	0.1501305014491265	No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATTAGATCGGAAGAGCACACGTCT	2692	0.14863968734867547	Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCCTGTGGGAATACTGCCAGCTGGGACTGAGAGATCGGAAGAGCACACGT	2678	0.14786667262992306	No Hit
AGGGGGCATGCCTGTTTGAGCGTCATTAGATCGGAAGAGCACACGTCTGAA	2616	0.14444332173259103	Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GAATACTGCCAGCTGGGACTGAGGACTGCGACGTAGATCGGAAGAGCACAC	2539	0.14019174077945284	No Hit
ATACTGCCAGCTGGGACTGAGGAAGATCGGAAGAGCACACGTCTGAACTCC	2436	0.13450456106291733	Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CTATGTTCCTTGGAACAGGACGTCAGATCGGAAGAGCACACGTCTGAACTC	2330	0.1286517353352206	Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ATAGATGGATACGAATAAGGCGTCAGATCGGAAGAGCACACGTCTGAACTC	2330	0.1286517353352206	Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGTCTGAACTCCAGTC	2287	0.12627747584190965	Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GGAATACTGCCAGCTGGGACTGAGGACTGCGACGTAGATCGGAAGAGCACA	2154	0.11893383601376188	No Hit
AGCCTGTGGGAATACTGCCAGCTGGGACTGAAGATCGGAAGAGCACACGTC	2125	0.11733259123920332	No Hit
ACTGCCAGCTGGGACTGAGGACTGCGACGTAGATCGGAAGAGCACACGTCT	2066	0.11407488635303252	Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTACGATCTGCTGAGATTAAGCCTTTGTTAGATCGGAAGAGCACACGTCTG	2001	0.11048588944453923	Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TAAGCCTTTGTTGTCTGATTTGTAGATCGGAAGAGCACACGTCTGAACTCC	1995	0.11015459742221678	Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGATTAAGCCTTTGTTGTCTGATTTGTAGATCGGAAGAGCACACGTCTGAA	1975	0.10905029068114193	Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCTGAGATTAAGCCTTTGTTGTCTGATTTGTAGATCGGAAGAGCACACGTC	1975	0.10905029068114193	No Hit
GAGATTAAGCCTTTGTTGTCTGATTTGTAGATCGGAAGAGCACACGTCTGA	1934	0.10678646186193846	Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCCAAGGACGTTTTCATTAATCAGATCGGAAGAGCACACGTCTGAACTCCA	1885	0.10408091034630508	Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AAAAAAATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGTCTG	1868	0.10314224961639143	Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGATGGATACGAATAAGGCGTCAGATCGGAAGAGCACACGTCTGAACTCCA	1850	0.10214837354942408	Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TAGCCTGTGGGAATACTGCCAGCTGGGACTGAGGAAGATCGGAAGAGCACC	1835	0.10132014349361794	No Hit
AAAAAATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGTCTGG	1815	0.10021583675254309	Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA Adapter	Nextera Transposase Sequence
1	0.07150386148459685	0.0	0.0
2	0.07282902957388668	0.0	0.0
3	0.0737676903038003	0.0	0.0
4	0.07398855165201527	0.0	0.0
5	0.07409898232612276	0.0	0.0
6	0.07431984367433773	0.0	0.0
7	0.07503764305603638	0.0	0.0
8	0.07564501176362756	0.0	0.0
9	0.0764732418194337	0.0	0.0
10	0.07774319457166978	0.0	0.0
11	0.07962051603149704	0.0	0.0
12	0.08602549512973119	0.0	0.0
13	0.09082922945340681	0.0	0.0
14	0.10286617293112273	0.0	0.0
15	0.1228541249445776	0.0	0.0
16	0.17326572767464474	0.0	0.0
17	0.2417879609583395	0.0	0.0
18	0.3104206249161417	0.0	0.0
19	0.5087541156131856	0.0	0.0
20	0.9969129105053253	0.0	0.0
21	4.045296453905409	0.0	0.0
22	5.427281125023535	0.0	0.0
23	7.648759780706768	0.0	0.0
24	9.731482294373944	0.0	0.0
25	12.178515601921715	0.0	0.0
26	14.501314401098565	0.0	0.0
27	19.107709110144107	0.0	0.0
28	24.078911551103726	0.0	0.0
29	33.58572263900599	0.0	0.0
30	44.807687741808664	0.0	0.0
31	59.89583074511441	0.0	0.0
32	70.33793442737002	0.0	0.0
33	77.32355801006133	0.0	0.0
34	82.48663374728271	0.0	0.0
35	90.6577306165179	0.0	0.0
36	94.77668433005299	0.0	0.0
37	95.09522160951603	0.0	0.0
38	95.1501608698845	0.0	0.0
39	95.19262146407884	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTGGACC	25	3.891698E-5	45.000004	10
TGGACCC	25	3.891698E-5	45.000004	11
GTGGGAC	20	7.0343626E-4	45.000004	7
GGGTACA	25	3.891698E-5	45.000004	1
GTCCTAT	45	3.8562575E-10	45.0	2
GGTCCTA	45	3.8562575E-10	45.0	1
ATGTAGT	45	3.8562575E-10	45.0	5
GTGTAGT	45	3.8562575E-10	45.0	10
CTCGCTA	35	1.2124474E-7	45.0	17
TCACTTT	45	3.8562575E-10	45.0	22
CATGTGG	30	2.166198E-6	44.999996	4
ATTCCAG	7330	0.0	44.57026	1
TTGCTCG	1445	0.0	44.37716	1
TAGCCTG	13040	0.0	44.120014	1
CGATATA	325	0.0	42.923077	3
ATAGCCT	235	0.0	42.12766	1
GCACACA	445	0.0	41.966293	45
GGCATGT	70	0.0	41.785713	10
ATATAGT	345	0.0	41.086956	5
CCGATAT	340	0.0	41.02941	2
>>END_MODULE