##FastQC	0.11.2
>>Basic Statistics	pass
#Measure	Value
Filename	000000000-AH0J7 l01n01 150804_gms7.3410000000ee9a.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2339830
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.31268510960198	33.0	32.0	34.0	32.0	34.0
2	32.59125833928106	33.0	32.0	34.0	32.0	34.0
3	32.74294115384451	33.0	32.0	34.0	32.0	34.0
4	32.813632614335226	33.0	32.0	34.0	32.0	34.0
5	32.602600616284086	33.0	32.0	34.0	32.0	34.0
6	35.36632020274977	37.0	35.0	37.0	33.0	37.0
7	35.28772560399687	37.0	34.0	37.0	33.0	37.0
8	35.07698849916447	37.0	34.0	37.0	32.0	37.0
9	35.46914733121637	37.0	35.0	37.0	33.0	37.0
10	35.50203348106486	37.0	35.0	37.0	33.0	37.0
11	35.38703623767539	37.0	35.0	37.0	33.0	37.0
12	35.41796882679511	37.0	35.0	37.0	33.0	37.0
13	36.76059414572853	38.0	38.0	38.0	35.0	38.0
14	36.80391011312788	38.0	38.0	38.0	35.0	38.0
15	36.75809481885436	38.0	38.0	38.0	34.0	38.0
16	36.834979036938584	38.0	38.0	38.0	35.0	38.0
17	36.750150224588964	38.0	38.0	38.0	35.0	38.0
18	36.82260206938111	38.0	38.0	38.0	35.0	38.0
19	36.828715334019996	38.0	38.0	38.0	35.0	38.0
20	36.80485719047965	38.0	38.0	38.0	35.0	38.0
21	36.87389767632691	38.0	38.0	38.0	36.0	38.0
22	36.89389699251655	38.0	38.0	38.0	36.0	38.0
23	37.364728206750065	39.0	38.0	39.0	35.0	39.0
24	37.25229354269327	39.0	38.0	39.0	35.0	39.0
25	37.252724770602995	38.0	38.0	39.0	35.0	39.0
26	37.150110050730184	38.0	38.0	39.0	35.0	39.0
27	37.01009987905104	38.0	37.0	39.0	34.0	39.0
28	36.83324728719608	38.0	37.0	39.0	34.0	39.0
29	36.760182577366734	38.0	37.0	39.0	34.0	39.0
30	36.82381925182599	38.0	37.0	39.0	34.0	39.0
31	36.968641311548275	38.0	37.0	39.0	34.0	39.0
32	37.137086454998865	38.0	38.0	39.0	35.0	39.0
33	37.148364624780434	38.0	38.0	39.0	35.0	39.0
34	36.982937649316405	38.0	38.0	39.0	34.0	39.0
35	36.934446519618945	38.0	38.0	39.0	34.0	39.0
36	36.95408170679067	38.0	38.0	39.0	35.0	39.0
37	36.97322583264596	38.0	38.0	38.0	35.0	39.0
38	36.9907262493429	38.0	38.0	39.0	35.0	39.0
39	37.216312723573935	38.0	38.0	39.0	35.0	39.0
40	37.389430856087834	39.0	38.0	39.0	36.0	39.0
41	37.46324690255275	39.0	38.0	39.0	36.0	39.0
42	37.60125479201481	39.0	38.0	39.0	36.0	39.0
43	37.482731651444766	39.0	38.0	39.0	36.0	39.0
44	37.52638225854015	39.0	38.0	39.0	36.0	39.0
45	37.38500788518824	39.0	38.0	39.0	36.0	39.0
46	37.13010987977759	38.0	38.0	39.0	34.0	39.0
47	37.16596376659843	39.0	38.0	39.0	34.0	39.0
48	37.27825953167538	39.0	38.0	39.0	36.0	39.0
49	37.33842202211272	39.0	38.0	39.0	36.0	39.0
50	37.18558613232585	38.0	38.0	39.0	36.0	39.0
51	36.484450579742976	39.0	37.0	39.0	33.0	39.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	-0.14881126771229702
1101	2	0.18988463346878603
1101	3	0.09897385342026865
1101	4	0.068879502259513
1101	5	0.17338391677955656
1101	6	0.07600410802882607
1101	7	0.06375682865268573
1101	8	0.13696223855224332
1101	9	-0.019696594802759648
1101	10	-0.07017809195685487
1101	11	-0.045161336019738485
1101	12	0.030341670624572714
1101	13	0.17273781420266232
1101	14	0.011553274809365632
1101	15	-0.025979224382368216
1101	16	-0.0301978416173867
1101	17	0.049484788113304035
1101	18	0.0685500769531373
1101	19	0.10223939281679861
1101	20	0.05766160618321692
1101	21	-0.01999588905250249
1101	22	-0.014838677485442986
1101	23	-0.05068711209779053
1101	24	0.014214416627147841
1101	25	0.06072715657225558
1101	26	-0.14607177467738808
1101	27	-0.006094069456253237
1101	28	-0.04940439978017963
1101	29	0.06923740655964394
1101	30	-0.010137503936896053
1101	31	0.053192151508895336
1101	32	-0.012921378510654336
1101	33	0.03890643305661712
1101	34	0.15822231831508304
1101	35	-0.03835832929964056
1101	36	0.0013259372934868452
1101	37	0.027734514408024324
1101	38	0.05873824084376622
1101	39	-0.0015013893637814135
1101	40	-0.053405426777743514
1101	41	0.015788253356106452
1101	42	-0.13689834061966621
1101	43	-0.01018588248513197
1101	44	-0.0368289051032491
1101	45	0.1452254625319256
1101	46	0.13997393720610063
1101	47	0.04685119937390425
1101	48	0.05865635757063359
1101	49	0.025110898023960715
1101	50	-0.05357703945431069
1101	51	0.5079837147696011
1102	1	0.31510709826482497
1102	2	0.0016211474340934728
1102	3	0.050353141601355844
1102	4	0.11274432089418696
1102	5	0.17813020431481874
1102	6	0.011541457519577136
1102	7	3.7004047574384913E-4
1102	8	0.16735377073389657
1102	9	-0.14297899210711051
1102	10	-0.24662511775146356
1102	11	0.00930109272947277
1102	12	-0.01265069418896303
1102	13	0.18204660711886334
1102	14	-0.10112006861345435
1102	15	0.050017675957718666
1102	16	-0.14433248886119543
1102	17	0.02131826814031257
1102	18	0.06174717253693274
1102	19	0.1367340787583302
1102	20	-0.032992773316763646
1102	21	-0.06794340497238238
1102	22	-0.16031104298204468
1102	23	0.09354007667977271
1102	24	-0.055569852355688454
1102	25	-0.048460166390299264
1102	26	-0.3425866021231698
1102	27	0.16178225674589441
1102	28	-0.2801265441689722
1102	29	-0.21791235191165015
1102	30	-0.3479209209816787
1102	31	-0.14551976061056138
1102	32	-0.0965468243728651
1102	33	-0.15697706014555024
1102	34	-0.01852491365707465
1102	35	0.12778663796881062
1102	36	-0.08870964867126219
1102	37	-0.08832333792540226
1102	38	-0.11661756543899315
1102	39	0.018976460839795095
1102	40	-0.14651888206249453
1102	41	-0.03410998012947175
1102	42	-0.1213582979895591
1102	43	0.20652031557765582
1102	44	-0.06203836754504266
1102	45	0.16425964891804057
1102	46	0.26014813624320965
1102	47	0.11689846746804022
1102	48	0.07302403667270596
1102	49	0.05920081259060339
1102	50	-0.08189327710422845
1102	51	0.5771275775070066
1103	1	0.1266645228410539
1103	2	0.17364447274685801
1103	3	0.08995641678450994
1103	4	0.06826585595126744
1103	5	0.14197233030918
1103	6	0.18328976114987228
1103	7	0.09400405154144664
1103	8	0.15273343093394942
1103	9	-0.16120693868935376
1103	10	-0.006307238803813675
1103	11	-0.06272629995610401
1103	12	-0.1000176482833055
1103	13	0.2095478884942139
1103	14	0.029154702298647805
1103	15	-0.033062328348194114
1103	16	-0.27956890414179725
1103	17	-0.015337293273347541
1103	18	-0.02645665116050111
1103	19	0.11075986651363934
1103	20	-0.13039999134385027
1103	21	-0.24355452009310596
1103	22	0.1010103822253896
1103	23	0.024434158621737367
1103	24	-0.10983435513924178
1103	25	-0.14718178805490112
1103	26	0.018142423994866874
1103	27	0.24016829874564394
1103	28	-0.4971931793568203
1103	29	-0.4564136442964255
1103	30	0.06150220322548705
1103	31	-0.20475960838684415
1103	32	-0.21720913946030862
1103	33	-0.3266885580274561
1103	34	0.1659749401621582
1103	35	-0.020245823947732333
1103	36	-0.15582209532139757
1103	37	-0.1833954558094888
1103	38	0.010494960723690383
1103	39	-0.12714173640270587
1103	40	-0.15720845772235492
1103	41	-0.10737186633844686
1103	42	0.05874038320303043
1103	43	0.04458496172845372
1103	44	-0.19463857476698365
1103	45	-0.03857072267469164
1103	46	0.14695541372121568
1103	47	-0.12142468621993885
1103	48	-0.2958468912143033
1103	49	-0.16542186977143558
1103	50	-0.015998289942452004
1103	51	0.24688867898827027
2112	1	0.3852651629899455
2112	2	0.2801680229178629
2112	3	0.13978494192429025
2112	4	0.15795565525287003
2112	5	0.024545392145384426
2112	6	0.13820589710017828
2112	7	0.09766273508577683
2112	8	0.06897424867870683
2112	9	0.13418134594007824
2112	10	0.08613201911626334
2112	11	-0.04808745420962168
2112	12	-0.26726457447421126
2112	13	0.11915375497078173
2112	14	-0.10100032329474828
2112	15	-0.16081696006821744
2112	16	-0.3493679784855459
2112	17	0.08512551990114048
2112	18	-0.07658164286260671
2112	19	0.09224774321560147
2112	20	-0.2316680590737974
2112	21	0.07229577333837511
2112	22	0.049439463787564364
2112	23	-0.11425039940102266
2112	24	-0.360994894122598
2112	25	0.09348916620591297
2112	26	-0.0030968208877979464
2112	27	0.18607494011096293
2112	28	-0.7821818254455124
2112	29	0.00585921019708735
2112	30	0.060416524702951335
2112	31	-0.36183682993492994
2112	32	-0.38338701281919185
2112	33	0.11251923080952508
2112	34	0.1690349194338694
2112	35	-0.039404854059966965
2112	36	-0.36125005435043533
2112	37	0.0770550465794102
2112	38	-0.04333748520842562
2112	39	-0.22491344533829505
2112	40	-0.2847739871638879
2112	41	0.073414397252904
2112	42	0.035298907128769486
2112	43	-0.11647801495192311
2112	44	-0.4699733761049316
2112	45	0.08843802554836344
2112	46	-0.13849997769717248
2112	47	-0.331252472772384
2112	48	-0.3692991171729858
2112	49	0.10413052537458611
2112	50	-0.10122343348854201
2112	51	0.04373097893127209
1113	1	0.1821501436506594
1113	2	0.04456719778232099
1113	3	0.1489765513735648
1113	4	0.10239663151347145
1113	5	0.09941329766285634
1113	6	0.16463724411943303
1113	7	0.07000705849424804
1113	8	0.022495769987521896
1113	9	0.10874462744913416
1113	10	0.14149297048341225
1113	11	0.029224931157379785
1113	12	0.20190078346879403
1113	13	0.25095418696426464
1113	14	0.1769216158911533
1113	15	0.09129491997978789
1113	16	0.057567997001008564
1113	17	0.12397489212369806
1113	18	-0.046519478688800575
1113	19	0.11241441437464772
1113	20	-0.029893938145185928
1113	21	0.16810124792069558
1113	22	0.122510301571225
1113	23	-0.08892806544794496
1113	24	0.11662227980345108
1113	25	0.14664610008811252
1113	26	0.14550931359792685
1113	27	0.32007258913373704
1113	28	0.10696051026112485
1113	29	0.33722987669226967
1113	30	0.2092683929985384
1113	31	-0.20173466350052394
1113	32	0.09202079936660823
1113	33	0.18304381490086996
1113	34	0.2488579543945164
1113	35	-0.13420840455020056
1113	36	0.07127501643218892
1113	37	0.20331211699641472
1113	38	0.09772358013115223
1113	39	-0.36573930519657694
1113	40	0.014960581004046958
1113	41	-0.0044319185361700875
1113	42	0.04962749632153418
1113	43	0.052437265335555594
1113	44	0.17693289930166856
1113	45	0.30956647626811673
1113	46	0.10029056694345684
1113	47	-0.3390174281720988
1113	48	-0.06894738217069829
1113	49	0.1619702501096114
1113	50	0.009779195550137842
1113	51	0.18568385049211855
2113	1	0.22670632209356967
2113	2	0.04652972597056504
2113	3	-0.05933394120614821
2113	4	-0.012129201634216713
2113	5	0.08697588693815561
2113	6	0.08218067780305205
2113	7	0.023548915284742122
2113	8	-0.2127055291597273
2113	9	0.06393949162494295
2113	10	0.00853063917663377
2113	11	-0.08943803104187253
2113	12	0.02924368950546352
2113	13	0.07573187964635508
2113	14	0.07438172984302582
2113	15	0.13340891081206507
2113	16	-0.02685280939130763
2113	17	0.1251898145019652
2113	18	0.012514608677832939
2113	19	-0.07359288609268333
2113	20	0.08395112474050848
2113	21	0.1492671746346943
2113	22	0.026922492133500953
2113	23	0.1694943140972356
2113	24	0.17042670851732566
2113	25	0.19780927310848284
2113	26	0.08155041305793986
2113	27	0.08424473909175845
2113	28	0.13755041841561422
2113	29	0.3186288934587864
2113	30	0.26467507666266954
2113	31	0.2125418555027423
2113	32	0.18098330134426988
2113	33	0.3254831420766422
2113	34	0.18010825703180444
2113	35	0.07593803722794235
2113	36	0.14059344891963832
2113	37	0.23135598781558286
2113	38	0.0995116827879059
2113	39	0.04504867440566329
2113	40	0.10522193101364508
2113	41	0.01950041523313928
2113	42	0.07497533104110943
2113	43	0.145681874550867
2113	44	0.18668291967888706
2113	45	0.26786515106851994
2113	46	0.1339652104025646
2113	47	0.23476290291733903
2113	48	0.086700251843709
2113	49	0.2709297437683347
2113	50	0.19217344915459478
2113	51	0.5669890385504601
1112	1	0.3277213311125422
1112	2	0.1615039800409619
1112	3	0.10353274057217021
1112	4	0.1513881721964978
1112	5	0.17570862786228503
1112	6	-0.07082727016288004
1112	7	0.025935653964339167
1112	8	-0.2618892453235375
1112	9	-0.18318313273002218
1112	10	-0.018203564777692804
1112	11	-0.1999682614644982
1112	12	0.007818321575470577
1112	13	0.004832856615898606
1112	14	0.25350032357525265
1112	15	0.15489953886788044
1112	16	-0.10662269686503123
1112	17	0.18187997828520963
1112	18	0.07080083958967975
1112	19	-0.04788476455102142
1112	20	-0.05531676078629033
1112	21	0.19592718106582652
1112	22	0.05732723103687931
1112	23	-0.05077816544157798
1112	24	0.16197205094032796
1112	25	0.21720396924217766
1112	26	-0.002462337196995179
1112	27	0.06789791646728105
1112	28	0.16766487125401142
1112	29	0.4035920232233394
1112	30	0.11819967886228255
1112	31	0.1481576790930461
1112	32	0.0425007358067333
1112	33	0.17512534533717172
1112	34	0.13013797700222796
1112	35	0.024493184984848426
1112	36	0.18958555024593693
1112	37	0.26513788037542696
1112	38	0.2041015350466182
1112	39	-0.08326919262497512
1112	40	-0.14482730335432592
1112	41	0.08135547249369068
1112	42	0.14094912377795765
1112	43	-0.4512180438392619
1112	44	-0.05875271885268063
1112	45	0.07002887715326267
1112	46	0.060967970188485765
1112	47	-0.023305661175918146
1112	48	-0.17333097582075396
1112	49	0.15308317634102764
1112	50	0.1523083929782345
1112	51	-0.1141858566196774
2114	1	0.3443739061929705
2114	2	-0.02993013185250959
2114	3	0.08122800932208918
2114	4	0.029094567718715325
2114	5	0.1604427137002773
2114	6	-0.047414919306099534
2114	7	-0.025997770964302447
2114	8	-0.5150760715041685
2114	9	-0.04637423521875661
2114	10	0.07062731839852887
2114	11	-0.051871794092626544
2114	12	-0.018636800384769003
2114	13	-0.21456276431903376
2114	14	0.21043875555143643
2114	15	0.1378468883067825
2114	16	0.07144202258159993
2114	17	0.20748044902790497
2114	18	0.07931534921468852
2114	19	0.06532185961067682
2114	20	0.12985958809068876
2114	21	0.05355576940629447
2114	22	0.1941462437956929
2114	23	0.06709673266474425
2114	24	0.29350445164784134
2114	25	0.22698558802361646
2114	26	0.17759935883702127
2114	27	0.2464370061438359
2114	28	0.2757617257957037
2114	29	0.18004801718767993
2114	30	0.2550316844821765
2114	31	0.18832715832173363
2114	32	0.1764984665032614
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2108	4	-0.05567363759850963
2108	5	-0.16103881604004755
2108	6	0.05151033186920273
2108	7	-0.2832662356258595
2108	8	-0.06490111019879663
2108	9	0.024982917683814776
2108	10	0.08004829876488628
2108	11	-0.18096454410387253
2108	12	0.10931379240046368
2108	13	-0.2785294698866494
2108	14	0.10515985695266039
2108	15	-0.05849820575331677
2108	16	0.058835916386556164
2108	17	-0.5064323751669448
2108	18	-0.02901702434041198
2108	19	-0.020266628661282482
2108	20	-0.1166264898577225
2108	21	-0.33621927012347186
2108	22	-0.03402284324437943
2108	23	-0.00599290797484997
2108	24	-0.013015226847997496
2108	25	-0.5369254715312834
2108	26	0.002626434723275395
2108	27	-0.04012082012168605
2108	28	-0.015172956809784921
2108	29	-1.0231650752972286
2108	30	-0.8196197399766021
2108	31	-0.3022049920240093
2108	32	-0.13256081478043313
2108	33	-0.39928595041959625
2108	34	-1.5558276660640686
2108	35	-0.6197492552985508
2108	36	-0.1681827587600253
2108	37	-0.2717895686580647
2108	38	-0.24678688037548824
2108	39	-0.21848696639042942
2108	40	-0.1619927900841347
2108	41	-1.1229879810510823
2108	42	-0.7216645340398458
2108	43	-0.1549740516912621
2108	44	-0.27812759162351597
2108	45	-0.5211268617156435
2108	46	-0.2191649693834421
2108	47	-0.1239083785963544
2108	48	-0.2357355169526869
2108	49	-0.7378852146778883
2108	50	-0.30134350611380967
2108	51	0.41999316061279757
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	2.0
17	1.0
18	2.0
19	9.0
20	14.0
21	56.0
22	170.0
23	659.0
24	1615.0
25	3599.0
26	6771.0
27	11555.0
28	17760.0
29	25051.0
30	34475.0
31	45684.0
32	59244.0
33	79825.0
34	111185.0
35	167512.0
36	333104.0
37	1418896.0
38	22641.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	19.998803331865993	43.676335460268476	29.688438903681035	6.636422304184491
2	21.50493839296017	43.909600270105095	23.645179350636585	10.94028198629815
3	16.309176307680474	50.789288110674704	17.584696324091922	15.3168392575529
4	20.13590730950539	51.843467260442	13.745571259450474	14.27505417060214
5	10.289251783249211	52.49368543868571	21.797139108396763	15.419923669668309
6	15.84281764059782	43.29951321249834	27.178470230743258	13.679198916160576
7	23.849852339699893	38.838462623352974	28.47360705692294	8.83807798002419
8	21.176538466469786	33.85027972117632	32.97354936042362	11.99963245193027
9	21.077428702085193	29.955723279041642	34.42493685438686	14.54191116448631
10	33.48230426996833	28.68281028963643	27.870101674053245	9.964783766341998
11	28.053191898556733	36.71950526320288	21.425231747605594	13.80207109063479
12	33.1978818974028	27.96797203215618	23.28989712927862	15.544248941162392
13	29.200711162776784	31.30966779637837	29.522187509348967	9.967433531495878
14	30.894509430172278	21.77799241825261	33.34353350457084	13.983964647004269
15	24.167012133360117	18.58588017078164	39.51505023869256	17.73205745716569
16	26.41110678980952	23.349046725616816	30.819461242910812	19.420385241662856
17	17.51866588598317	28.629686772115924	32.13024023112791	21.721407110773004
18	16.132026685699387	34.50874636191518	27.814242915083575	21.544984037301855
19	23.585132253197884	39.17258946162755	17.492467401477885	19.74981088369668
20	19.03676762841745	42.788578657423834	21.769829432052756	16.404824282105963
21	23.87408486941359	38.33522093485424	20.39195155203583	17.398742643696337
22	33.085095925772386	31.58532884867704	16.216605479885292	19.112969745665286
23	34.536526157883266	32.41201283853955	13.175102464708974	19.876358538868207
24	31.260177021407536	30.05961971596227	14.933349858750422	23.74685340387977
25	39.92623395716783	28.994585076693607	13.468884491608366	17.6102964745302
26	38.30893697405367	25.55783967211293	16.351444335699604	19.781779018133797
27	38.78333041289325	21.92595188539338	20.717958142258198	18.572759559455175
28	32.20498925135587	23.978964283730015	23.117876085014725	20.698170379899395
29	30.870020471572722	43.54029993632016	15.094686366103522	10.494993226003599
30	40.34241803891735	34.14735258544424	18.72896748909109	6.781261886547313
31	27.672907860827493	49.161563019535606	14.667091198933255	8.498437920703642
32	27.987588841924417	30.683382980814844	30.126504917023887	11.202523260236855
33	26.66462948162901	25.658744438698537	17.496014667732272	30.180611411940184
34	46.960206510729414	22.25623229038007	12.04779834432416	18.735762854566357
35	52.1758418346632	25.799053777411178	5.193197796421107	16.831906591504513
36	38.30256899005483	48.08302312561169	3.329771821029733	10.284636063303743
37	30.356094246163185	55.952440989302644	2.0887414897663508	11.602723274767826
38	43.585987016150746	44.13200959044033	1.5313078300560299	10.750695563352892
39	31.761580969557617	50.863524273130956	2.7797318608616868	14.595162896449743
40	43.862887474731075	34.038455785249354	2.7400708598487924	19.358585880170782
41	24.823769248193244	29.741690635644467	5.526939991366894	39.90760012479539
42	19.73596372386028	41.44758379882299	5.434668330605215	33.38178414671151
43	12.082971839834517	31.8104306723138	7.09688310689238	49.0097143809593
44	13.3765273545514	44.21462242983464	7.804669570011496	34.604180645602455
45	14.69611040118299	26.390293311907275	11.098797775906796	47.814798511002934
46	31.719355679686128	20.749669847809457	16.271353047016234	31.259621425488177
47	20.621498143027484	13.058940179414744	36.35862434450366	29.96093733305411
48	18.437963441788504	11.778847181205473	29.329780368659264	40.45340900834676
49	15.101054350102357	14.86945632802383	42.05579037793344	27.973698943940374
50	32.11746152498258	19.615100242325298	24.784834795690287	23.482603437001835
51	19.21814832701521	40.36750533158392	20.27937927114363	20.13496707025724
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	11.0
1	8.5
2	6.0
3	5.5
4	5.0
5	5.5
6	6.0
7	10.0
8	14.0
9	12.0
10	10.0
11	13.5
12	17.0
13	21.0
14	25.0
15	27.0
16	29.0
17	40.0
18	51.0
19	63.0
20	75.0
21	91.5
22	108.0
23	117.5
24	127.0
25	167.0
26	263.0
27	319.0
28	394.5
29	470.0
30	685.0
31	900.0
32	1243.0
33	1586.0
34	3519.5
35	5453.0
36	16818.5
37	28184.0
38	44717.0
39	61250.0
40	83910.0
41	106570.0
42	369822.0
43	633074.0
44	546822.5
45	460571.0
46	359019.5
47	257468.0
48	250988.0
49	244508.0
50	215868.5
51	187229.0
52	164509.0
53	141789.0
54	118398.5
55	95008.0
56	82756.0
57	70504.0
58	47497.0
59	24490.0
60	17887.5
61	11285.0
62	8091.0
63	4897.0
64	3553.5
65	2210.0
66	1524.0
67	838.0
68	581.5
69	325.0
70	209.5
71	94.0
72	74.5
73	55.0
74	52.0
75	28.5
76	8.0
77	5.0
78	2.0
79	2.0
80	2.0
81	1.0
82	0.0
83	0.5
84	1.0
85	0.5
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2339830.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	22.96108987563471
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.72530924476985	18.076189010562967
2	10.02477033221196	4.6035930516103045
3	3.711956862620655	2.5569172541133574
4	2.072797203493072	1.903747315334749
5	1.22600565791214	1.4075213049678652
6	0.7504787485370393	1.0339085996947694
7	0.5254796176706629	0.8445909310405177
8	0.36916174001401736	0.6781084712886037
9	0.28815958879450126	0.5954812395152836
>10	1.8701083647847412	8.53476497320266
>50	0.23267912418249875	3.694884129288075
>100	0.16998635951726238	7.922500096053366
>500	0.015430139459595083	2.3961701710053696
>1k	0.012839391696695538	6.065944740767581
>5k	0.001674562270011384	2.2363670673579388
>10k+	0.003163062065577059	37.449311644196555
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TGAAAAAATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGTCT	182086	7.782018351760597	Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAAAAATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGTCTGA	164439	7.027818260300962	Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GAAAAAATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGTCTG	146758	6.272165071821457	Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAAAATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGTCTGAA	101937	4.356598556305372	Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AAAATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGTCTGAAC	84758	3.622399917942756	Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TTGAAAAAATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGTC	32479	1.3880922973036502	No Hit
AGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGTCTGAACTCCAGT	23276	0.994773124543236	Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AAATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGTCTGAACT	19552	0.8356162627199413	Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATTAGATCGGAAGAGCACACGT	16573	0.7082993208908339	No Hit
TTCCAGGGGGCATGCCTGTTTGAGCGTCATTAGATCGGAAGAGCACACGTC	15728	0.6721855861323259	No Hit
ATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGTCTGAACTCC	14970	0.6397900702187767	Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATTCCAGGGGGCATGCCTGTTTGAGCGTCAGATCGGAAGAGCACACGTCTG	14336	0.6126940846129847	Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGTCTGAACTCCA	13971	0.5970946607232149	Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGTCTGAACTC	13278	0.567477124406474	Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TTGATGATTCATAATAACTTTTCGAATCGCAAGATCGGAAGAGCACACGTC	12084	0.5164477761204874	No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTAGATCGGAAGAGCACACG	10186	0.4353307718936846	No Hit
TTGCTCGAATATATTAGCATGGAATAGATCGGAAGAGCACACGTCTGAACT	10171	0.4346896996790365	Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATTCCAGGGGGCATGCCTGTTTGAGCGTAGATCGGAAGAGCACACGTCTGA	7113	0.3039964441861161	Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTGCTCGAATATATTAGCATGGAATAATAGATCGGAAGAGCACACGTCTGA	6380	0.27266938196364693	Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTCCAGGGGGCATGCCTGTTTGAGCGTCAGATCGGAAGAGCACACGTCTGA	6201	0.26501925353551326	Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCAGTCTCATAATCTGAAGGTCGAGAGTAGATCGGAAGAGCACACGTCTGA	5938	0.25377912070535036	Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTTGATGATTCATAATAACTTTTCGAATCGCAAGATCGGAAGAGCACACGT	5615	0.2399746990165952	No Hit
TTCCAGGGGGCATGCCTGTTTGAGCGTCATTTAGATCGGAAGAGCACACGT	5490	0.23463243056119462	No Hit
TCCGATATAGTGTAACGGCTATCACATCAGATCGGAAGAGCACACGTCTGA	5414	0.23138433134031106	Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTTGAAAAAATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGT	5114	0.2185628870473496	No Hit
TAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGTCTGAACTCCAG	5082	0.21719526632276703	Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GCCAAGGACGTTTTCATTAATCAAGAACAGATCGGAAGAGCACACGTCTGA	4956	0.21181025971972323	Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCCGATATAGTGTAACGGCTATCACATCACGAGATCGGAAGAGCACACGTC	4784	0.204459298325092	No Hit
ATGCCTGTTTGAGCGTCATTTCCTTCTCAAACAGATCGGAAGAGCACACGT	4745	0.20279251056700703	No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAATAGATCGGAAGAGCACACG	4400	0.18804784963010135	No Hit
AATTCTAGAGCTAATACATGCTAGATCGGAAGAGCACACGTCTGAACTCCA	3733	0.1595415051520837	Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACAC	3500	0.14958351675121698	No Hit
AAAAAATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGTCTGG	3419	0.14612172679211738	Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
AGTTTTGGTGGCAGGATAAATCCAAGATCGGAAGAGCACACGTCTGAACTC	3408	0.14565160716804212	Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AAGCCTTTGTTGTCTGATTTGTAGATCGGAAGAGCACACGTCTGAACTCCA	3302	0.1411213635178624	Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ATAGATGGATACGAATAAGGCGTCAGATCGGAAGAGCACACGTCTGAACTC	3192	0.13642016727710987	Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGATGATTCATAATAACTTTTCGAATCGCAAGATCGGAAGAGCACACGTCT	3151	0.13466790322373848	Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTGCTCGAATATATTAGCATGGAATAATAGAAAGATCGGAAGAGCACACGT	2958	0.12641944072859992	No Hit
AAAAAATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGTCTGC	2889	0.1234705085412188	Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
AGAGCTACCAACTGTAGAAAGAGAAAGTGAGATCGGAAGAGCACACGTCTG	2817	0.12039336191090805	Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAAAATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGTCTGAC	2734	0.11684609565652206	Illumina Multiplexing PCR Primer 2.01 (95% over 23bp)
AGGGGGCATGCCTGTTTGAGCGTCATTAGATCGGAAGAGCACACGTCTGAA	2728	0.11658966677066283	Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TAGCCTGTGGGAATACTGCCAGCTGGGACTGAGGAAGATCGGAAGAGCACA	2448	0.10462298543056547	No Hit
ACTGCCAGCTGGGACTGAGGACTGCGACGAGATCGGAAGAGCACACGTCTG	2399	0.10252881619604844	Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA Adapter	Nextera Transposase Sequence
1	0.058636738566477054	0.0	0.0
2	0.06372257813601843	0.0	0.0
3	0.06581674737053547	0.0	0.0
4	0.06654329588046995	0.0	0.0
5	0.06782544030976609	0.0	0.0
6	0.06915032288670545	0.0	0.0
7	0.07081711064479043	0.0	0.0
8	0.0749199728185381	0.0	0.0
9	0.0783817627776377	0.0	0.0
10	0.08543355713876649	0.0	0.0
11	0.09449404443912592	0.0	0.0
12	0.1181709782334614	0.0	0.0
13	0.14411303385288673	0.0	0.0
14	0.1895864229452567	0.0	0.0
15	0.253693644410064	0.0	0.0
16	0.33904172525354404	0.0	0.0
17	0.43498886671253895	0.0	0.0
18	0.5402529243577525	0.0	0.0
19	0.7284717265784266	0.0	0.0
20	1.1040118299192676	0.0	0.0
21	2.9587192231914283	0.0	0.0
22	4.693845279357902	0.0	0.0
23	7.697866939051128	0.0	0.0
24	10.617993614920742	0.0	0.0
25	13.952252941453011	0.0	0.0
26	17.857408444203212	0.0	0.0
27	24.34830735566259	0.0	0.0
28	33.59150023719672	0.0	0.0
29	60.232794690212536	0.0	0.0
30	75.6643431360398	0.0	0.0
31	86.51718287225995	0.0	0.0
32	90.89164597428018	0.0	0.0
33	93.46324305611947	0.0	0.0
34	94.67204882405987	0.0	0.0
35	95.1087044785305	0.0	0.0
36	95.28991422453768	0.0	0.0
37	95.32363462302817	0.0	0.0
38	95.34876465384237	0.0	0.0
39	95.37671540240103	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGCCTG	1025	0.0	43.463417	1
TTGCTCG	2860	0.0	43.26923	1
ATTCCAG	6155	0.0	42.367992	1
CAGTCTC	750	0.0	42.0	2
TCAGTCT	765	0.0	41.764706	1
TGCTCGA	3005	0.0	41.181362	2
TAATCGC	110	0.0	40.909092	10
GTCCTAT	100	0.0	40.500004	2
TCTCGCT	100	0.0	40.500004	16
CGTCTGC	1380	0.0	40.27174	45
GCTCGAA	3075	0.0	40.170734	3
GTCTCAT	805	0.0	39.40994	4
CTCGAAT	3140	0.0	39.339172	4
GCGACTG	115	0.0	39.130436	2
TGGGCCA	250	0.0	38.699997	1
CGTCTGG	1170	0.0	38.653847	45
TCTCATA	815	0.0	38.650307	5
ATGTAGT	170	0.0	38.38235	5
ATATAGT	1735	0.0	38.25648	5
CGATATA	1745	0.0	38.166187	3
>>END_MODULE