FastQCFastQC Report
Wed 5 Aug 2015
000000000-AH0J7 l01n01 150804_gms7.3410000000ee9a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AH0J7 l01n01 150804_gms7.3410000000ee9a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2339830
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAAAAAATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGTCT1820867.782018351760597Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAAAAATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGTCTGA1644397.027818260300962Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GAAAAAATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGTCTG1467586.272165071821457Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAAAATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGTCTGAA1019374.356598556305372Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AAAATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGTCTGAAC847583.622399917942756Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TTGAAAAAATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGTC324791.3880922973036502No Hit
AGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGTCTGAACTCCAGT232760.994773124543236Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AAATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGTCTGAACT195520.8356162627199413Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATTAGATCGGAAGAGCACACGT165730.7082993208908339No Hit
TTCCAGGGGGCATGCCTGTTTGAGCGTCATTAGATCGGAAGAGCACACGTC157280.6721855861323259No Hit
ATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGTCTGAACTCC149700.6397900702187767Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATTCCAGGGGGCATGCCTGTTTGAGCGTCAGATCGGAAGAGCACACGTCTG143360.6126940846129847Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGTCTGAACTCCA139710.5970946607232149Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGTCTGAACTC132780.567477124406474Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TTGATGATTCATAATAACTTTTCGAATCGCAAGATCGGAAGAGCACACGTC120840.5164477761204874No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTAGATCGGAAGAGCACACG101860.4353307718936846No Hit
TTGCTCGAATATATTAGCATGGAATAGATCGGAAGAGCACACGTCTGAACT101710.4346896996790365Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATTCCAGGGGGCATGCCTGTTTGAGCGTAGATCGGAAGAGCACACGTCTGA71130.3039964441861161Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTGCTCGAATATATTAGCATGGAATAATAGATCGGAAGAGCACACGTCTGA63800.27266938196364693Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTCCAGGGGGCATGCCTGTTTGAGCGTCAGATCGGAAGAGCACACGTCTGA62010.26501925353551326Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCAGTCTCATAATCTGAAGGTCGAGAGTAGATCGGAAGAGCACACGTCTGA59380.25377912070535036Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTTGATGATTCATAATAACTTTTCGAATCGCAAGATCGGAAGAGCACACGT56150.2399746990165952No Hit
TTCCAGGGGGCATGCCTGTTTGAGCGTCATTTAGATCGGAAGAGCACACGT54900.23463243056119462No Hit
TCCGATATAGTGTAACGGCTATCACATCAGATCGGAAGAGCACACGTCTGA54140.23138433134031106Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTTGAAAAAATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGT51140.2185628870473496No Hit
TAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGTCTGAACTCCAG50820.21719526632276703Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GCCAAGGACGTTTTCATTAATCAAGAACAGATCGGAAGAGCACACGTCTGA49560.21181025971972323Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCCGATATAGTGTAACGGCTATCACATCACGAGATCGGAAGAGCACACGTC47840.204459298325092No Hit
ATGCCTGTTTGAGCGTCATTTCCTTCTCAAACAGATCGGAAGAGCACACGT47450.20279251056700703No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAATAGATCGGAAGAGCACACG44000.18804784963010135No Hit
AATTCTAGAGCTAATACATGCTAGATCGGAAGAGCACACGTCTGAACTCCA37330.1595415051520837Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACAC35000.14958351675121698No Hit
AAAAAATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGTCTGG34190.14612172679211738Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
AGTTTTGGTGGCAGGATAAATCCAAGATCGGAAGAGCACACGTCTGAACTC34080.14565160716804212Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AAGCCTTTGTTGTCTGATTTGTAGATCGGAAGAGCACACGTCTGAACTCCA33020.1411213635178624Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ATAGATGGATACGAATAAGGCGTCAGATCGGAAGAGCACACGTCTGAACTC31920.13642016727710987Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGATGATTCATAATAACTTTTCGAATCGCAAGATCGGAAGAGCACACGTCT31510.13466790322373848Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTGCTCGAATATATTAGCATGGAATAATAGAAAGATCGGAAGAGCACACGT29580.12641944072859992No Hit
AAAAAATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGTCTGC28890.1234705085412188Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
AGAGCTACCAACTGTAGAAAGAGAAAGTGAGATCGGAAGAGCACACGTCTG28170.12039336191090805Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAAAATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGTCTGAC27340.11684609565652206Illumina Multiplexing PCR Primer 2.01 (95% over 23bp)
AGGGGGCATGCCTGTTTGAGCGTCATTAGATCGGAAGAGCACACGTCTGAA27280.11658966677066283Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TAGCCTGTGGGAATACTGCCAGCTGGGACTGAGGAAGATCGGAAGAGCACA24480.10462298543056547No Hit
ACTGCCAGCTGGGACTGAGGACTGCGACGAGATCGGAAGAGCACACGTCTG23990.10252881619604844Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCCTG10250.043.4634171
TTGCTCG28600.043.269231
ATTCCAG61550.042.3679921
CAGTCTC7500.042.02
TCAGTCT7650.041.7647061
TGCTCGA30050.041.1813622
TAATCGC1100.040.90909210
GTCCTAT1000.040.5000042
TCTCGCT1000.040.50000416
CGTCTGC13800.040.2717445
GCTCGAA30750.040.1707343
GTCTCAT8050.039.409944
CTCGAAT31400.039.3391724
GCGACTG1150.039.1304362
TGGGCCA2500.038.6999971
CGTCTGG11700.038.65384745
TCTCATA8150.038.6503075
ATGTAGT1700.038.382355
ATATAGT17350.038.256485
CGATATA17450.038.1661873
Produced by FastQC (version 0.11.2)