##FastQC	0.11.2
>>Basic Statistics	pass
#Measure	Value
Filename	000000000-AH0J7 l01n01 150804_gms6.3410000000ee8d.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1334775
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.434517428030944	33.0	32.0	34.0	32.0	34.0
2	32.64454009102658	33.0	32.0	34.0	32.0	34.0
3	32.758539079620164	33.0	32.0	34.0	32.0	34.0
4	32.85670543724598	33.0	32.0	34.0	32.0	35.0
5	32.683519694330506	33.0	32.0	34.0	32.0	35.0
6	35.52410106572269	37.0	35.0	37.0	33.0	37.0
7	35.39276132681538	37.0	35.0	37.0	33.0	37.0
8	35.088076267535726	37.0	34.0	37.0	33.0	37.0
9	35.58367590043266	37.0	35.0	37.0	33.0	37.0
10	35.70436215841621	37.0	37.0	37.0	33.0	37.0
11	35.50385121087824	37.0	35.0	37.0	33.0	37.0
12	35.45999138431571	37.0	35.0	37.0	33.0	37.0
13	36.76816916708809	38.0	38.0	38.0	35.0	38.0
14	36.84439549736847	38.0	38.0	38.0	35.0	38.0
15	36.71511153565208	38.0	37.0	38.0	34.0	38.0
16	36.86572043977449	38.0	38.0	38.0	35.0	38.0
17	36.82937573748384	38.0	38.0	38.0	35.0	38.0
18	36.9108096870259	38.0	38.0	38.0	36.0	38.0
19	36.93118540578	38.0	38.0	38.0	36.0	38.0
20	36.926403326403324	38.0	38.0	38.0	36.0	38.0
21	36.96592983836227	38.0	38.0	38.0	36.0	38.0
22	36.96404562566725	38.0	38.0	38.0	36.0	38.0
23	37.442216853027666	39.0	38.0	39.0	36.0	39.0
24	37.328117472982335	39.0	38.0	39.0	35.0	39.0
25	37.27822441984604	38.0	38.0	39.0	35.0	39.0
26	37.12972448540016	38.0	38.0	39.0	35.0	39.0
27	36.84520237493211	38.0	37.0	39.0	34.0	39.0
28	36.67568691352475	38.0	37.0	38.0	34.0	39.0
29	36.64018280234497	38.0	37.0	38.0	34.0	39.0
30	36.69033282763012	38.0	37.0	38.0	34.0	39.0
31	36.84853477177801	38.0	37.0	39.0	34.0	39.0
32	37.02155794047686	38.0	38.0	39.0	35.0	39.0
33	37.058956753010804	38.0	38.0	39.0	35.0	39.0
34	37.05193984004795	38.0	38.0	39.0	34.0	39.0
35	37.1624498510985	38.0	38.0	39.0	35.0	39.0
36	37.131748796613664	38.0	38.0	39.0	35.0	39.0
37	37.00740499335094	38.0	38.0	39.0	35.0	39.0
38	37.057105504673075	38.0	38.0	39.0	35.0	39.0
39	37.1341544455058	38.0	38.0	39.0	35.0	39.0
40	37.22712067576933	38.0	38.0	39.0	36.0	39.0
41	37.32099192747842	39.0	38.0	39.0	36.0	39.0
42	37.44857372965481	39.0	38.0	39.0	36.0	39.0
43	37.413005188140325	39.0	38.0	39.0	36.0	39.0
44	37.52896705437246	39.0	38.0	39.0	36.0	39.0
45	37.434885280290686	39.0	38.0	39.0	36.0	39.0
46	37.424971999026056	39.0	38.0	39.0	36.0	39.0
47	37.24287164503381	39.0	38.0	39.0	35.0	39.0
48	37.18096608042554	39.0	38.0	39.0	35.0	39.0
49	37.35237624318705	39.0	38.0	39.0	36.0	39.0
50	37.37866756569459	39.0	38.0	39.0	36.0	39.0
51	36.66395160232998	39.0	37.0	39.0	33.0	39.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	-0.10169438033633327
1101	2	0.20287983753623706
1101	3	0.07353267208618064
1101	4	0.13978619141546034
1101	5	0.1990353089657475
1101	6	0.2537373811380732
1101	7	0.18901739416656937
1101	8	0.27876118595475674
1101	9	-0.045218379578571444
1101	10	0.004942091946425364
1101	11	-0.0068538586035984395
1101	12	0.09281824258717819
1101	13	0.21987563625611983
1101	14	0.1252298923569839
1101	15	0.06710751275417692
1101	16	0.09601797974476511
1101	17	0.08493181556259088
1101	18	0.09899762658075417
1101	19	0.1062684441235362
1101	20	0.10549224859258999
1101	21	0.16732954970845526
1101	22	0.04207950888318379
1101	23	-0.10354833276763742
1101	24	0.007103545732611849
1101	25	0.19437585650902633
1101	26	-0.10383356692775436
1101	27	0.07818504772723855
1101	28	-0.05098240535808429
1101	29	0.17519834613644747
1101	30	0.01408315763057999
1101	31	0.16249637030679764
1101	32	0.15253728745312856
1101	33	0.12849849959681592
1101	34	0.2262192906856484
1101	35	0.03506769390954645
1101	36	0.19188483857697491
1101	37	0.10544215638929444
1101	38	0.19288423059810356
1101	39	0.07431823160246154
1101	40	0.019259411847507124
1101	41	0.19587387119573663
1101	42	-0.149978882362916
1101	43	0.014431216011523418
1101	44	-0.08523221889516464
1101	45	0.2654687480255902
1101	46	0.204261333375932
1101	47	0.1582031938224091
1101	48	0.10600768399545757
1101	49	0.02120224707578444
1101	50	0.03226046029597285
1101	51	0.507233901642806
1102	1	0.2215937833202304
1102	2	0.015393544572397388
1102	3	0.021668696718904812
1102	4	0.12413493472533332
1102	5	0.1623654470164837
1102	6	0.0029312984819185317
1102	7	0.02138941946739692
1102	8	0.1686777091968068
1102	9	-0.15926128350949398
1102	10	-0.3266258477498809
1102	11	-0.03558907134406297
1102	12	0.003050408916159597
1102	13	0.04097829923368579
1102	14	-0.07204812627732338
1102	15	0.10085859297755206
1102	16	-0.13846474757417582
1102	17	0.020909608348510744
1102	18	-0.036158470236308915
1102	19	0.1818386931377205
1102	20	-0.02138606923636388
1102	21	-0.14837199637260312
1102	22	-0.12089497242804725
1102	23	0.18775323655184195
1102	24	0.03972883348959755
1102	25	0.01791782727219271
1102	26	-0.32140707658259515
1102	27	0.09588237458286386
1102	28	-0.3022459943866451
1102	29	-0.2695855365676323
1102	30	-0.3049783370354717
1102	31	-0.12906826517681225
1102	32	-0.12444378775946774
1102	33	-0.2243758023428697
1102	34	-0.0818043180955712
1102	35	0.11591978137315095
1102	36	-0.04430609408244379
1102	37	-0.04020808362000139
1102	38	-0.10809614019991898
1102	39	-0.029226171932677403
1102	40	-0.1872478900994352
1102	41	-0.051722586397779935
1102	42	-0.27383293783011453
1102	43	0.16896742800400233
1102	44	-0.09455191418139464
1102	45	0.11904292578497433
1102	46	0.10650983195620967
1102	47	0.11254659955794466
1102	48	0.07006093628480414
1102	49	0.036926850025615465
1102	50	-0.2063919292670704
1102	51	0.4125095673871968
1103	1	0.06439279931357333
1103	2	0.1964158901089732
1103	3	0.04659017324697601
1103	4	0.0614883768520329
1103	5	0.16577047455405136
1103	6	0.10406860515689687
1103	7	0.12416750820378297
1103	8	0.18450272143212487
1103	9	-0.055597625791342864
1103	10	-0.013880065813374642
1103	11	-0.09883185250435389
1103	12	-0.1062938611139117
1103	13	0.22421581619892095
1103	14	-0.060592627228857054
1103	15	0.013092711807736634
1103	16	-0.22571517245670236
1103	17	-0.06977498680343075
1103	18	-0.06025525371136098
1103	19	0.1174397319142102
1103	20	-0.16361846725761353
1103	21	-0.2119422863289131
1103	22	0.14447532067382696
1103	23	0.04355301025621827
1103	24	-0.1625799184891008
1103	25	-0.15250798967259271
1103	26	0.029050556824046225
1103	27	0.13942032073264699
1103	28	-0.29626872756782063
1103	29	-0.4340501845835689
1103	30	0.10987090252425702
1103	31	-0.09300848279438156
1103	32	-0.23352503307003758
1103	33	-0.24985728190548429
1103	34	0.1337370382164238
1103	35	0.02522339035355259
1103	36	-0.16072293506582014
1103	37	-0.1942293642904147
1103	38	0.03397384491415778
1103	39	-0.16475143960636274
1103	40	-0.1997242636704044
1103	41	-0.024996989735036834
1103	42	0.05702176761087685
1103	43	-0.004939881455726436
1103	44	-0.1853179702075991
1103	45	-0.025610863186493305
1103	46	0.09667457628193432
1103	47	-0.13094259033887568
1103	48	-0.19136102549288125
1103	49	-0.11753638248620035
1103	50	0.023594507798044617
1103	51	0.20619294964640034
2112	1	0.3444109255864163
2112	2	0.17672409054537042
2112	3	0.09298794058585003
2112	4	0.11351686465227573
2112	5	-0.038329669439534086
2112	6	0.12392662251256326
2112	7	-0.06703109274700125
2112	8	0.06306082104332944
2112	9	0.10904406541533262
2112	10	0.014267646521503252
2112	11	-0.06990592182385313
2112	12	-0.2451052425062059
2112	13	0.027582533460602576
2112	14	-0.12062298980254837
2112	15	-0.16169930261462184
2112	16	-0.5348262820012764
2112	17	0.06511469355752553
2112	18	-0.17742168372792122
2112	19	0.05883687593635756
2112	20	-0.3144234548302407
2112	21	-0.02019429476298029
2112	22	-0.029604981070832537
2112	23	-0.2387828895589763
2112	24	-0.4255724082646637
2112	25	-0.014858834248201447
2112	26	-0.02459456596378118
2112	27	0.1258765388549108
2112	28	-0.969540248041362
2112	29	0.022927187535401572
2112	30	0.05320774881621304
2112	31	-0.3266301866825785
2112	32	-0.45795471682213673
2112	33	0.05341389722794787
2112	34	0.096324075306363
2112	35	-0.1322349466745436
2112	36	-0.44897264501948797
2112	37	0.09247513238318561
2112	38	-0.15339210025103966
2112	39	-0.2400021390753153
2112	40	-0.3280307640846445
2112	41	-0.01807001165983735
2112	42	-0.05434252984625232
2112	43	-0.13073210587646145
2112	44	-0.46737755149459304
2112	45	0.025417535197483687
2112	46	-0.04506082836748959
2112	47	-0.38589160451524407
2112	48	-0.4813573672173703
2112	49	-0.008936800776247367
2112	50	-0.12673775155068512
2112	51	-0.09801450314385107
1113	1	0.15745645883158232
1113	2	0.014886607879390112
1113	3	0.136706179807355
1113	4	0.0722867280320969
1113	5	0.11659520004872803
1113	6	0.19793804489157196
1113	7	0.09975247281411015
1113	8	-0.05001494404502438
1113	9	-0.017741274623531922
1113	10	0.20453063799890003
1113	11	0.06671709001257398
1113	12	0.16425609520174334
1113	13	0.19523918954125463
1113	14	0.10943083967379152
1113	15	0.014448101718748774
1113	16	0.029078284686590905
1113	17	0.044860910378261565
1113	18	-0.1578891928942312
1113	19	0.14348612813638084
1113	20	0.026061948812333924
1113	21	0.09898379343075447
1113	22	0.09772584657333994
1113	23	-0.12738685387372328
1113	24	0.15125636266485998
1113	25	0.20344108827357843
1113	26	0.13953257719276024
1113	27	0.1880847632306839
1113	28	0.006223799724949686
1113	29	0.2835124402378284
1113	30	0.2765192930719067
1113	31	-0.18449780480035827
1113	32	0.08646299889192477
1113	33	0.14097223409685
1113	34	0.1628428018885515
1113	35	-0.08668855722559954
1113	36	0.14088813150074486
1113	37	0.0746196325610029
1113	38	-0.001942067134756087
1113	39	-0.43862580520477223
1113	40	-0.01072803598940908
1113	41	-0.0876532722736556
1113	42	-0.0410924570050355
1113	43	-0.01844974411229572
1113	44	0.13498160937459147
1113	45	0.31532393332165043
1113	46	0.1321254304565187
1113	47	-0.26699657473250227
1113	48	-0.05712081253753354
1113	49	0.19905755010445603
1113	50	0.051765308510638874
1113	51	-0.001762503205192445
2113	1	0.21517245193496848
2113	2	0.05960320579054468
2113	3	-0.08165885327652944
2113	4	0.0014064167817693374
2113	5	0.024089490482836595
2113	6	0.07372118068445843
2113	7	0.02910469661104287
2113	8	-0.030749740351183164
2113	9	0.09096335350995588
2113	10	0.1508401370916843
2113	11	0.0041305889609333235
2113	12	0.04548123715103003
2113	13	0.13428575973957635
2113	14	0.04736745220866112
2113	15	0.017857953766522883
2113	16	-0.0080474340868264
2113	17	0.18582788065241118
2113	18	-0.006944699804385834
2113	19	0.04922340686249527
2113	20	0.06403078767168324
2113	21	0.14746491349863788
2113	22	0.022001566700801334
2113	23	0.03990920404339704
2113	24	0.12094881340011199
2113	25	0.21809224056778476
2113	26	0.15413579621441187
2113	27	0.04906473235840991
2113	28	0.17398357368932693
2113	29	0.3588547885168367
2113	30	0.27498696293712754
2113	31	0.1887812462833267
2113	32	0.13105890605844905
2113	33	0.38139262063220514
2113	34	0.12228024039223584
2113	35	0.11657451610057024
2113	36	0.1770344227653311
2113	37	0.2931879532716053
2113	38	0.18504969123100068
2113	39	0.1130338956410597
2113	40	0.1716590590376299
2113	41	0.10117303957245127
2113	42	0.16035754705711014
2113	43	0.2723031964269751
2113	44	0.17310309449361228
2113	45	0.27611442610457715
2113	46	0.17211297322185004
2113	47	0.3184620410701342
2113	48	0.19558737222672562
2113	49	0.21031284217244917
2113	50	0.21096082390615578
2113	51	0.5915279218753753
1112	1	0.2920713438451443
1112	2	0.173768869986219
1112	3	0.11952304867953956
1112	4	0.10851594108847706
1112	5	0.1434022568942268
1112	6	-0.09038406288194523
1112	7	-0.03179988782122933
1112	8	-0.3608632730287127
1112	9	-0.26752613075843357
1112	10	-0.04202251151991021
1112	11	-0.172214711984644
1112	12	0.019150644157278407
1112	13	-0.1335271587447764
1112	14	0.24078437270854636
1112	15	-0.010442778453423784
1112	16	-0.26468644011981013
1112	17	0.05279636296427981
1112	18	-0.04307532820616444
1112	19	-0.17657756368074473
1112	20	-0.024514940893972437
1112	21	0.17785322158483297
1112	22	0.027129194498833442
1112	23	-0.05137311146032886
1112	24	0.05900990587408472
1112	25	0.15399171807233358
1112	26	0.026456111799845416
1112	27	0.0840029271842937
1112	28	0.06538005739936636
1112	29	0.2505023514909297
1112	30	0.02168902250433291
1112	31	0.12596748799307989
1112	32	0.12787268250665562
1112	33	0.14945080783174802
1112	34	0.15590279984822786
1112	35	-0.09403282244107203
1112	36	0.06781312710372589
1112	37	0.1822474160682006
1112	38	0.13709377877054862
1112	39	-0.20608875687450023
1112	40	-0.11949510662656593
1112	41	0.06910339469685312
1112	42	0.09678138120225555
1112	43	-0.5546814464739356
1112	44	-0.19301350688003538
1112	45	0.06455910559379419
1112	46	-0.00840888669853257
1112	47	-0.10584112431715198
1112	48	-0.3007023199503678
1112	49	0.0798417337648587
1112	50	0.11513070982874041
1112	51	-0.18583463367664876
2114	1	0.3639885180812996
2114	2	-0.08951062577864377
2114	3	0.05480217527045994
2114	4	-0.00664180304075046
2114	5	0.07959717072802874
2114	6	-0.18796776047523878
2114	7	-0.13240889973950942
2114	8	-0.5218365574152273
2114	9	-0.0964761489669641
2114	10	0.015660871971761026
2114	11	-0.025354781152046257
2114	12	-0.021000640321439334
2114	13	-0.28065472281504356
2114	14	0.08453360210708638
2114	15	0.10481614899799752
2114	16	-0.03863476791910614
2114	17	0.04997913336990223
2114	18	0.06882136455998733
2114	19	0.013612907557458698
2114	20	0.18067250690615566
2114	21	0.04814330545843859
2114	22	0.08121554439066614
2114	23	-0.10624051618897568
2114	24	0.2180429721370558
2114	25	0.11144321529960166
2114	26	0.04194077857301437
2114	27	0.21264860918937245
2114	28	0.20263445219533338
2114	29	0.036641773085065665
2114	30	0.1912794181208497
2114	31	0.27536825936291365
2114	32	0.16182122830799273
2114	33	0.1706668285512336
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2108	2	-0.1592530415232858
2108	3	-0.07986729532265713
2108	4	-0.028469596363613903
2108	5	-0.1810806658465296
2108	6	0.04797159419103991
2108	7	-0.2528092419251706
2108	8	-0.07884561309968774
2108	9	-0.03236653574408166
2108	10	0.0621046206751501
2108	11	-0.09932372127536837
2108	12	0.1716047811954482
2108	13	-0.2466244322750768
2108	14	0.18866410465040673
2108	15	-0.0606228599623293
2108	16	0.1323049473203568
2108	17	-0.35782469567808306
2108	18	0.03626775792243109
2108	19	0.05454663659850212
2108	20	-0.08630305648517833
2108	21	-0.2502314749935053
2108	22	0.08678157251811314
2108	23	-0.004824480354493232
2108	24	0.017702059589552732
2108	25	-0.4449236441626425
2108	26	0.04188823058516533
2108	27	0.02034501818804557
2108	28	0.08977722413843026
2108	29	-0.7426249355516319
2108	30	-0.6831425112908818
2108	31	-0.15587329664538174
2108	32	-0.07745898217105207
2108	33	-0.25930289526338157
2108	34	-1.30457732666963
2108	35	-0.4345463580588529
2108	36	-0.024981448941268525
2108	37	-0.1365573440407104
2108	38	-0.124876984610232
2108	39	-0.05154315550352351
2108	40	-0.04561913429677844
2108	41	-0.9751317093498244
2108	42	-0.6172344771498501
2108	43	-0.05292899674245888
2108	44	-0.2360647262290385
2108	45	-0.3841508692975779
2108	46	-0.12865300159006665
2108	47	-0.006110712210784186
2108	48	-0.03510232635844801
2108	49	-0.44826334939939727
2108	50	-0.17718124002347935
2108	51	0.5270223715534712
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	1.0
16	2.0
17	1.0
18	1.0
19	2.0
20	3.0
21	25.0
22	74.0
23	325.0
24	847.0
25	1894.0
26	3577.0
27	6289.0
28	9732.0
29	13545.0
30	18892.0
31	25135.0
32	33291.0
33	44414.0
34	62040.0
35	94375.0
36	189102.0
37	823740.0
38	7468.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	19.545766140360733	39.992433181622374	36.59021183345508	3.871588844561818
2	25.40458129647319	48.00299675975352	20.90636998745107	5.686051956322227
3	14.479294263078046	63.828435504111184	13.917102133318348	7.775168099492424
4	9.984828903747822	66.1015526961473	8.272255623606975	15.64136277649791
5	6.528291285048042	64.71240471240472	15.440504954018467	13.31879904852878
6	11.449195557303666	59.710513007810306	22.436215841621244	6.404075593264783
7	19.640164072596505	50.45389672416699	25.35393605663876	4.552003146597741
8	19.607799067258526	38.30593171133712	36.12309190687569	5.963177314528665
9	22.830814182165536	29.35775692532449	41.419040662283905	6.392388230226068
10	34.3869191436759	28.323275458410595	31.57108876027795	5.7187166376355565
11	35.14390065741417	40.08765522279036	16.690528366204042	8.077915753591428
12	41.220430409619595	31.49242381674814	16.67157386076305	10.61557191286921
13	29.879942312374748	36.70416362308254	28.339308123091904	5.076585941450807
14	38.55563671779888	14.935101421587907	39.54112116274278	6.968140697870427
15	29.08924725140941	14.199247064111928	43.74692363881553	12.964582045663125
16	27.32520462250192	16.018804667453317	35.60862317619075	21.04736753385402
17	13.391545391545392	21.14828341855369	44.000599351950704	21.459571837950218
18	14.523271712460902	30.591672753834914	33.6948923975951	21.19016313610908
19	18.760465246951732	38.94573991871289	14.099754640295181	28.19404019404019
20	15.044258395609747	51.70459440729711	17.195182708696223	16.05596448839692
21	21.26530688692851	50.19834803618587	15.985390796201607	12.55095428068401
22	33.865033432601	34.374332752711126	13.353861137644921	18.406772677042948
23	42.0733082354704	25.917476728287543	7.275533329587383	24.73368170665468
24	31.19372178831638	29.614354479219347	10.089191062164035	29.102732670300234
25	46.14710344440074	29.36397520181304	7.986214905133824	16.502706448652393
26	50.97308535146373	16.22003708490195	11.680095896312112	21.12678166732221
27	49.611882152422695	16.082710569197054	15.019834803618586	19.285572474761665
28	30.813208218613624	18.523721226423927	23.292390103200912	27.37068045176153
29	35.20720720720721	29.166114139087114	19.910771478339047	15.715907175366633
30	27.5441179224963	34.52020003371355	31.570114813358057	6.365567230432095
31	29.559888370699184	43.047554831338616	18.32061583412935	9.071940963832855
32	34.9556292258995	34.33005562735293	20.884343803262723	9.829971343484857
33	28.013485418890827	33.98902436740275	21.706205165664624	16.291285048041804
34	34.09675788054166	23.92612987207582	22.484688430634375	19.492423816748143
35	41.41866606731471	26.176097095016015	8.354067164877977	24.051169672791296
36	49.931936094098255	33.12917907512502	6.209660804255399	10.729224026521324
37	40.68843063437658	44.12713753254294	4.782229214661647	10.402202618418835
38	35.25006087168249	52.426513831919245	2.7009046468505926	9.622520649547676
39	36.34657526549419	48.39489801651964	2.5148807851510555	12.74364593283512
40	43.680470491281305	40.08106235133262	1.7280815118652957	14.510385645520781
41	30.75649454027832	41.62162162162162	3.0554213256915963	24.566462512408457
42	31.075649454027833	35.18720383585248	3.2424191343110262	30.494727575808657
43	15.758910677829597	35.60832350021539	4.396546234384072	44.23621958757094
44	16.154183289318425	42.99054147702797	5.029237137345246	35.826038096308366
45	15.435185705455975	30.712442171901632	9.213238186211159	44.639133936431236
46	19.498791931224364	31.76894982300388	11.695229533067371	37.03702871270439
47	22.34031952950872	16.421569178325935	22.34136839542245	38.8967428967429
48	28.207375774943344	13.066996310239553	27.82798599014815	30.89764192466895
49	16.166619842295518	15.771197392819014	37.66065441741118	30.401528347474294
50	21.316626397707477	18.08147440579873	28.018130396508774	32.583768799985016
51	22.730872244385758	26.111142327358543	29.953812440298925	21.20417298795677
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	4.0
1	5.5
2	7.0
3	6.0
4	5.0
5	3.5
6	2.0
7	3.5
8	5.0
9	7.5
10	10.0
11	11.0
12	12.0
13	13.5
14	15.0
15	20.5
16	26.0
17	33.0
18	40.0
19	46.0
20	52.0
21	57.5
22	63.0
23	82.5
24	102.0
25	114.0
26	175.0
27	224.0
28	270.0
29	316.0
30	434.5
31	553.0
32	776.5
33	1000.0
34	1938.5
35	2877.0
36	11266.5
37	19656.0
38	22961.0
39	26266.0
40	40378.0
41	54490.0
42	287317.0
43	520144.0
44	410448.0
45	300752.0
46	182794.0
47	64836.0
48	63424.0
49	62012.0
50	55303.0
51	48594.0
52	68008.5
53	87423.0
54	73310.5
55	59198.0
56	56980.5
57	54763.0
58	36679.5
59	18596.0
60	13105.0
61	7614.0
62	5119.0
63	2624.0
64	1952.0
65	1280.0
66	962.5
67	645.0
68	479.0
69	313.0
70	185.5
71	58.0
72	43.0
73	28.0
74	26.0
75	18.0
76	12.0
77	8.5
78	5.0
79	4.0
80	3.0
81	1.5
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1334775.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	11.056709494909304
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.18235040445298	8.754962455461508
2	9.641010733558824	2.131957098365247
3	3.674073955152217	1.2186950515479147
4	1.9688874269530408	0.8707766523199694
5	1.1325257567454405	0.6261004143918328
6	0.6259539345408237	0.41525944868480175
7	0.5010853966857307	0.38782489643068596
8	0.2976007490860624	0.26323880224895896
9	0.2625752567453921	0.26128965009465227
>10	1.9883663569836902	4.466832369738687
>50	0.3328390442888176	2.5990410609470223
>100	0.30972526999497446	6.692837201090877
>500	0.03605986063055272	2.65754622664231
>1k	0.029936486724401915	5.851085022635141
>5k	0.010885995172509764	8.703920586391318
>10k+	0.006123372284536742	54.098633063009096
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TGAAAAAATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGTCT	215505	16.145417767039387	Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GAAAAAATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGTCTG	154735	11.59259051150943	Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAAAAATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGTCTGA	127444	9.547976250678953	Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AAAAATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGTCTGAA	69571	5.212189320297429	Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AAAATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGTCTGAAC	59270	4.440448764773089	Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TTGAAAAAATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGTC	37298	2.79432863216647	No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTAGATCGGAAGAGCACACG	24096	1.8052480755183458	No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATTAGATCGGAAGAGCACACGT	18631	1.3958157741941526	No Hit
AAATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGTCTGAACT	12588	0.9430802944316459	Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TAGCCTGTGGGAATACTGCCAGCTGGGACTGAGGAAGATCGGAAGAGCACA	9812	0.7351051675376	No Hit
TAGCCTGTGGGAATACTGCCAGCTGGGACTGAGGAGATCGGAAGAGCACAC	9447	0.7077597347867618	No Hit
TTCCAGGGGGCATGCCTGTTTGAGCGTCATTTAGATCGGAAGAGCACACGT	9309	0.6974209136371299	No Hit
TTCCAGGGGGCATGCCTGTTTGAGCGTCATTAGATCGGAAGAGCACACGTC	9036	0.6769680283193796	No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCAGATCGGAAGAGCACACGTCTG	7816	0.5855668558371261	Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGTCTGAACTC	7311	0.5477327639489802	Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGTCTGAACTCC	6897	0.5167163005000842	Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTGATGATTCATAATAACTTTTCGAATCGCAAGATCGGAAGAGCACACGTC	6779	0.5078758592272106	No Hit
TTTGAAAAAATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGT	6595	0.4940907643610346	No Hit
ACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACAC	6534	0.4895207057369219	No Hit
TTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGTCTGAACTCCA	6481	0.4855499990635126	Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TAGCCTGTGGGAATACTGCCAGCTGGGACTAGATCGGAAGAGCACACGTCT	6386	0.47843269464891086	Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GAAAAAATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGTCTA	6189	0.463673652862842	Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
TAGCCTGTGGGAATACTGCCAGCTGGGACTGAGATCGGAAGAGCACACGTC	6139	0.4599277031709464	No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCAAGATCGGAAGAGCACACGT	5616	0.42074506939371803	No Hit
AGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGTCTGAACTCCAGT	5355	0.40119121200202285	Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CTTTGAAAAAATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACG	4491	0.3364612013260662	No Hit
TCCGATATAGTGTAACGGCTATCACATCACGAGATCGGAAGAGCACACGTC	4079	0.3055945758648461	No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAATAGATCGGAAGAGCACACG	3712	0.27809930512633213	No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAAAGATCGGAAGAGCACACGT	2940	0.22026184188346348	No Hit
AAAAAATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGTCTGG	2899	0.2171901631361091	Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
AATACTGCCAGCTGGGACTGAGGACTGCGACGAGATCGGAAGAGCACACGT	2676	0.20048322751025455	No Hit
ATGCCTGTTTGAGCGTCATTTCCTTCTCAAACAGATCGGAAGAGCACACGT	2327	0.17433649866082299	No Hit
TAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGTCTGAACTCCAG	2324	0.17411174167930923	Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TTCCAGGGGGCATGCCTGTTTGAGCGTCAGATCGGAAGAGCACACGTCTGA	2242	0.16796838418460042	Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATTCCAGGGGGCATGCCTGTTTGAGCGTAGATCGGAAGAGCACACGTCTGA	2162	0.1619748646775674	Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AAGCCTTTGTTGTCTGATTTGTAGATCGGAAGAGCACACGTCTGAACTCCA	1968	0.14744057987301232	Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCAGTCTCATAATCTGAAGGTCGAGAGTAGATCGGAAGAGCACACGTCTGA	1956	0.14654155194695737	Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TAGCCTGTGGGAATACTGCCAGCTGGGACTGAGAGATCGGAAGAGCACACG	1879	0.14077278942143806	No Hit
GCTGAGATTAAGCCTTTGTTGTCTGATTTGTAGATCGGAAGAGCACACGTC	1868	0.13994868048922104	No Hit
AAAAATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGTCTGAC	1742	0.13050888726564402	Illumina Multiplexing PCR Primer 2.01 (95% over 23bp)
ATAGATGGATACGAATAAGGCGTCAGATCGGAAGAGCACACGTCTGAACTC	1724	0.1291603453765616	Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGCCTGTGGGAATACTGCCAGCTGGGACTGAGGAAGATCGGAAGAGCACAC	1713	0.12833623644434455	No Hit
ACTGCCAGCTGGGACTGAGGACTGCGACGAGATCGGAAGAGCACACGTCTG	1707	0.12788672248131708	Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACGATCAACTTAGAACTGGTACGGACAAGAGATCGGAAGAGCACACGTCTG	1694	0.12691277556142422	Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTGCTCGAATATATTAGCATGGAATAGATCGGAAGAGCACACGTCTGAACT	1635	0.12249255492498737	Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATCGAATCTTTGAACGCACATTGCGCCCCTTAGATCGGAAGAGCACACGTC	1595	0.11949579517147085	No Hit
TAAGCCTTTGTTGTCTGATTTGTAGATCGGAAGAGCACACGTCTGAACTCC	1526	0.11432638459665485	Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTGCTCGAATATATTAGCATGGAATAATAGATCGGAAGAGCACACGTCTGA	1523	0.11410162761514113	Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATACTGCCAGCTGGGACTGAGGACTGCGACGAGATCGGAAGAGCACACGTC	1510	0.11312768069524826	No Hit
GTCAAGGATGCTGGCATAATGGTTATATGCCAGATCGGAAGAGCACACGTC	1487	0.11140454383697627	No Hit
GAGATTAAGCCTTTGTTGTCTGATTTGTAGATCGGAAGAGCACACGTCTGA	1471	0.11020583993556966	Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGCCTGTGGGAATACTGCCAGCTGGGACTGAGGAGATCGGAAGAGCACACG	1462	0.10953156899102844	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA Adapter	Nextera Transposase Sequence
1	0.04165496057387949	0.0	0.0
2	0.04622501919799217	0.0	0.0
3	0.04922177895150868	0.0	0.0
4	0.0498960498960499	0.0	0.0
5	0.05094491580978067	0.0	0.0
6	0.0516941057481598	0.0	0.0
7	0.052518214680376846	0.0	0.0
8	0.05394167556329719	0.0	0.0
9	0.05506546047086588	0.0	0.0
10	0.05701335431065161	0.0	0.0
11	0.05828697720589612	0.0	0.0
12	0.060984060984060985	0.0	0.0
13	0.06240752186698133	0.0	0.0
14	0.06428049671292914	0.0	0.0
15	0.06705249948493192	0.0	0.0
16	0.070423854207638	0.0	0.0
17	0.07446947987488528	0.0	0.0
18	0.08061283736959413	0.0	0.0
19	0.09761944897080033	0.0	0.0
20	0.15373377535539698	0.0	0.0
21	0.8120469742091364	0.0	0.0
22	1.549100035586522	0.0	0.0
23	3.164353542731921	0.0	0.0
24	4.86718735367384	0.0	0.0
25	6.923488977543031	0.0	0.0
26	9.397838587027776	0.0	0.0
27	15.653874248468844	0.0	0.0
28	23.215972729486243	0.0	0.0
29	37.341799179637015	0.0	0.0
30	54.65460470865876	0.0	0.0
31	75.16892360135603	0.0	0.0
32	82.45719315989587	0.0	0.0
33	87.67994605832443	0.0	0.0
34	91.5245640651046	0.0	0.0
35	93.39199490550843	0.0	0.0
36	94.53975389110525	0.0	0.0
37	94.7795695903804	0.0	0.0
38	94.90872993575697	0.0	0.0
39	95.02477945721189	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATGGCAT	60	0.0	45.000004	13
TATGGCA	60	0.0	45.000004	12
CCACTAC	30	2.1657652E-6	45.000004	20
GTCGGTT	60	0.0	45.000004	6
TTGTAAG	90	0.0	45.0	14
TGGCATC	55	1.8189894E-12	45.0	14
TGTAAGC	90	0.0	45.0	15
GGTCCTA	65	0.0	44.999996	1
TTGCTCG	1205	0.0	44.626556	1
ATTCCAG	6360	0.0	44.257072	1
ATAGCCT	225	0.0	44.0	1
TGGGCCA	180	0.0	43.75	1
CGTCTGG	395	0.0	43.29114	45
CCGATGT	125	0.0	43.199997	2
CGATGTA	125	0.0	43.199997	3
TAGCCTG	3985	0.0	42.233376	1
TCGAGAG	240	0.0	42.187504	21
TGCTCGA	1275	0.0	42.176468	2
GGTACAC	150	0.0	42.0	2
CGAGAGT	225	0.0	42.0	22
>>END_MODULE