FastQCFastQC Report
Wed 5 Aug 2015
000000000-AH0J7 l01n01 150804_gms6.3410000000ee8d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AH0J7 l01n01 150804_gms6.3410000000ee8d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1334775
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAAAAAATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGTCT21550516.145417767039387Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GAAAAAATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGTCTG15473511.59259051150943Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAAAAATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGTCTGA1274449.547976250678953Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AAAAATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGTCTGAA695715.212189320297429Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AAAATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGTCTGAAC592704.440448764773089Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TTGAAAAAATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGTC372982.79432863216647No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTAGATCGGAAGAGCACACG240961.8052480755183458No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATTAGATCGGAAGAGCACACGT186311.3958157741941526No Hit
AAATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGTCTGAACT125880.9430802944316459Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TAGCCTGTGGGAATACTGCCAGCTGGGACTGAGGAAGATCGGAAGAGCACA98120.7351051675376No Hit
TAGCCTGTGGGAATACTGCCAGCTGGGACTGAGGAGATCGGAAGAGCACAC94470.7077597347867618No Hit
TTCCAGGGGGCATGCCTGTTTGAGCGTCATTTAGATCGGAAGAGCACACGT93090.6974209136371299No Hit
TTCCAGGGGGCATGCCTGTTTGAGCGTCATTAGATCGGAAGAGCACACGTC90360.6769680283193796No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCAGATCGGAAGAGCACACGTCTG78160.5855668558371261Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGTCTGAACTC73110.5477327639489802Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGTCTGAACTCC68970.5167163005000842Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTGATGATTCATAATAACTTTTCGAATCGCAAGATCGGAAGAGCACACGTC67790.5078758592272106No Hit
TTTGAAAAAATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGT65950.4940907643610346No Hit
ACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACAC65340.4895207057369219No Hit
TTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGTCTGAACTCCA64810.4855499990635126Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TAGCCTGTGGGAATACTGCCAGCTGGGACTAGATCGGAAGAGCACACGTCT63860.47843269464891086Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GAAAAAATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGTCTA61890.463673652862842Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
TAGCCTGTGGGAATACTGCCAGCTGGGACTGAGATCGGAAGAGCACACGTC61390.4599277031709464No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCAAGATCGGAAGAGCACACGT56160.42074506939371803No Hit
AGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGTCTGAACTCCAGT53550.40119121200202285Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CTTTGAAAAAATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACG44910.3364612013260662No Hit
TCCGATATAGTGTAACGGCTATCACATCACGAGATCGGAAGAGCACACGTC40790.3055945758648461No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAATAGATCGGAAGAGCACACG37120.27809930512633213No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAAAGATCGGAAGAGCACACGT29400.22026184188346348No Hit
AAAAAATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGTCTGG28990.2171901631361091Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
AATACTGCCAGCTGGGACTGAGGACTGCGACGAGATCGGAAGAGCACACGT26760.20048322751025455No Hit
ATGCCTGTTTGAGCGTCATTTCCTTCTCAAACAGATCGGAAGAGCACACGT23270.17433649866082299No Hit
TAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGTCTGAACTCCAG23240.17411174167930923Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TTCCAGGGGGCATGCCTGTTTGAGCGTCAGATCGGAAGAGCACACGTCTGA22420.16796838418460042Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATTCCAGGGGGCATGCCTGTTTGAGCGTAGATCGGAAGAGCACACGTCTGA21620.1619748646775674Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AAGCCTTTGTTGTCTGATTTGTAGATCGGAAGAGCACACGTCTGAACTCCA19680.14744057987301232Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCAGTCTCATAATCTGAAGGTCGAGAGTAGATCGGAAGAGCACACGTCTGA19560.14654155194695737Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TAGCCTGTGGGAATACTGCCAGCTGGGACTGAGAGATCGGAAGAGCACACG18790.14077278942143806No Hit
GCTGAGATTAAGCCTTTGTTGTCTGATTTGTAGATCGGAAGAGCACACGTC18680.13994868048922104No Hit
AAAAATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGTCTGAC17420.13050888726564402Illumina Multiplexing PCR Primer 2.01 (95% over 23bp)
ATAGATGGATACGAATAAGGCGTCAGATCGGAAGAGCACACGTCTGAACTC17240.1291603453765616Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGCCTGTGGGAATACTGCCAGCTGGGACTGAGGAAGATCGGAAGAGCACAC17130.12833623644434455No Hit
ACTGCCAGCTGGGACTGAGGACTGCGACGAGATCGGAAGAGCACACGTCTG17070.12788672248131708Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACGATCAACTTAGAACTGGTACGGACAAGAGATCGGAAGAGCACACGTCTG16940.12691277556142422Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTGCTCGAATATATTAGCATGGAATAGATCGGAAGAGCACACGTCTGAACT16350.12249255492498737Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATCGAATCTTTGAACGCACATTGCGCCCCTTAGATCGGAAGAGCACACGTC15950.11949579517147085No Hit
TAAGCCTTTGTTGTCTGATTTGTAGATCGGAAGAGCACACGTCTGAACTCC15260.11432638459665485Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTGCTCGAATATATTAGCATGGAATAATAGATCGGAAGAGCACACGTCTGA15230.11410162761514113Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATACTGCCAGCTGGGACTGAGGACTGCGACGAGATCGGAAGAGCACACGTC15100.11312768069524826No Hit
GTCAAGGATGCTGGCATAATGGTTATATGCCAGATCGGAAGAGCACACGTC14870.11140454383697627No Hit
GAGATTAAGCCTTTGTTGTCTGATTTGTAGATCGGAAGAGCACACGTCTGA14710.11020583993556966Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGCCTGTGGGAATACTGCCAGCTGGGACTGAGGAGATCGGAAGAGCACACG14620.10953156899102844No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGGCAT600.045.00000413
TATGGCA600.045.00000412
CCACTAC302.1657652E-645.00000420
GTCGGTT600.045.0000046
TTGTAAG900.045.014
TGGCATC551.8189894E-1245.014
TGTAAGC900.045.015
GGTCCTA650.044.9999961
TTGCTCG12050.044.6265561
ATTCCAG63600.044.2570721
ATAGCCT2250.044.01
TGGGCCA1800.043.751
CGTCTGG3950.043.2911445
CCGATGT1250.043.1999972
CGATGTA1250.043.1999973
TAGCCTG39850.042.2333761
TCGAGAG2400.042.18750421
TGCTCGA12750.042.1764682
GGTACAC1500.042.02
CGAGAGT2250.042.022
Produced by FastQC (version 0.11.2)