##FastQC	0.11.2
>>Basic Statistics	pass
#Measure	Value
Filename	000000000-AH0J7 l01n01 150804_gms5.3410000000ee70.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3130522
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.373950095223734	33.0	32.0	34.0	32.0	34.0
2	32.64346648897532	33.0	32.0	34.0	32.0	34.0
3	32.73505440945632	33.0	32.0	34.0	32.0	34.0
4	32.818126817188954	33.0	32.0	34.0	32.0	35.0
5	32.6481273730068	33.0	32.0	34.0	32.0	35.0
6	35.61898079617393	37.0	37.0	37.0	33.0	37.0
7	35.43378931692542	37.0	35.0	37.0	33.0	37.0
8	34.76009336462098	37.0	34.0	37.0	32.0	37.0
9	35.0069972356048	37.0	34.0	37.0	32.0	37.0
10	35.18024182548469	37.0	34.0	37.0	33.0	37.0
11	35.01920159002237	37.0	34.0	37.0	33.0	37.0
12	34.94148419975966	37.0	34.0	37.0	32.0	37.0
13	36.79681152216787	38.0	38.0	38.0	35.0	38.0
14	36.864557731905414	38.0	38.0	38.0	35.0	38.0
15	36.74416950272191	38.0	38.0	38.0	34.0	38.0
16	36.88974490516278	38.0	38.0	38.0	36.0	38.0
17	36.842910543353476	38.0	38.0	38.0	35.0	38.0
18	36.88115336675481	38.0	38.0	38.0	36.0	38.0
19	36.84388386345792	38.0	38.0	38.0	35.0	38.0
20	36.880352222408916	38.0	38.0	38.0	36.0	38.0
21	36.93584488465502	38.0	38.0	38.0	36.0	38.0
22	36.9281135861687	38.0	38.0	38.0	36.0	38.0
23	37.352997040110246	39.0	38.0	39.0	35.0	39.0
24	37.25083867802239	39.0	38.0	39.0	35.0	39.0
25	37.272039934554044	38.0	38.0	39.0	35.0	39.0
26	37.166123732719335	38.0	38.0	39.0	35.0	39.0
27	36.86680975249495	38.0	37.0	39.0	34.0	39.0
28	36.76757326733369	38.0	37.0	39.0	34.0	39.0
29	36.76041759169876	38.0	37.0	39.0	34.0	39.0
30	36.89670157245341	38.0	37.0	39.0	34.0	39.0
31	37.05643244161836	38.0	37.0	39.0	35.0	39.0
32	37.128935046615226	38.0	38.0	39.0	35.0	39.0
33	37.1203438276428	38.0	38.0	39.0	35.0	39.0
34	36.992529360918084	38.0	37.0	39.0	34.0	39.0
35	37.08655169968459	38.0	38.0	39.0	35.0	39.0
36	37.154515125592475	38.0	38.0	39.0	35.0	39.0
37	37.10089946660653	38.0	38.0	39.0	35.0	39.0
38	37.08325831921961	38.0	38.0	39.0	35.0	39.0
39	37.13923460688026	38.0	38.0	39.0	35.0	39.0
40	37.224624838924626	38.0	38.0	39.0	36.0	39.0
41	37.20058220322362	38.0	38.0	39.0	35.0	39.0
42	37.24028229157949	38.0	38.0	39.0	35.0	39.0
43	37.14074393982857	39.0	38.0	39.0	34.0	39.0
44	37.30649616900951	39.0	38.0	39.0	35.0	39.0
45	37.253069935301525	39.0	38.0	39.0	36.0	39.0
46	37.25301882561438	39.0	38.0	39.0	36.0	39.0
47	37.22349818975877	39.0	38.0	39.0	34.0	39.0
48	37.281301329299076	39.0	38.0	39.0	36.0	39.0
49	37.31709887360638	39.0	38.0	39.0	36.0	39.0
50	37.27728570506772	39.0	38.0	39.0	36.0	39.0
51	36.5757011131051	39.0	37.0	39.0	33.0	39.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	-0.11284001940611432
1101	2	0.18563542920068699
1101	3	0.074684246141139
1101	4	0.059616476646127126
1101	5	0.2009854425383324
1101	6	0.11325612931597107
1101	7	0.09859000514032346
1101	8	0.2513168248924984
1101	9	0.03566088746693197
1101	10	-0.08597261161029479
1101	11	0.01681304910131587
1101	12	0.09655795312954751
1101	13	0.22308860655754614
1101	14	0.08299446137043986
1101	15	-0.034097879972748046
1101	16	-0.03338616391271643
1101	17	0.04064393625125007
1101	18	0.11180490950327027
1101	19	0.14606865976121952
1101	20	0.08567194977856474
1101	21	0.028325507933267602
1101	22	0.00612964232547597
1101	23	-0.011071774382848787
1101	24	-3.3288110392959425E-4
1101	25	0.1239307317168965
1101	26	-0.17994354466058127
1101	27	0.01580706096546436
1101	28	-0.018566274334069988
1101	29	0.07940101728985383
1101	30	-0.05568978434239824
1101	31	0.07035003705894383
1101	32	0.028085043796465925
1101	33	0.0719652849687833
1101	34	0.16586012465901234
1101	35	-0.10347597070871473
1101	36	-0.02106652193324976
1101	37	0.07209798684847613
1101	38	0.033526539106986775
1101	39	0.007040392688821839
1101	40	-0.008102945586635713
1101	41	0.03130601982227432
1101	42	-0.10520545483188926
1101	43	0.057462150514787425
1101	44	-0.029873584007461318
1101	45	0.152933719241382
1101	46	0.24821053611643862
1101	47	0.1464498439265327
1101	48	0.0822621984765064
1101	49	0.09221315995360868
1101	50	-0.01591331766466908
1101	51	0.5324157136233154
1102	1	0.32303140192058777
1102	2	-0.021880911778758616
1102	3	0.09544372508246113
1102	4	0.11656168767332531
1102	5	0.15640046665293994
1102	6	-0.03844826896490616
1102	7	0.06203279469769285
1102	8	0.19632700819188642
1102	9	-0.11246624400344274
1102	10	-0.27019721653567785
1102	11	-0.002730793970137313
1102	12	0.052772350876331586
1102	13	0.019185434064105777
1102	14	-0.13116810582423
1102	15	0.07660278634014617
1102	16	-0.08254765471997416
1102	17	0.015190849732086065
1102	18	0.0034688238186220133
1102	19	0.20938389521431588
1102	20	-0.11180694392219692
1102	21	-0.06669116385033647
1102	22	-0.18077092584236
1102	23	0.11740443041249904
1102	24	0.013963442824667993
1102	25	-0.06740876004496954
1102	26	-0.3505961396822386
1102	27	0.0554724716010071
1102	28	-0.33128196506316954
1102	29	-0.222617125841019
1102	30	-0.34122606729818017
1102	31	-0.08803728453405313
1102	32	-0.11903965787701054
1102	33	-0.19317965828974337
1102	34	-0.06963488172698362
1102	35	0.07454296373386171
1102	36	-0.10857014047911662
1102	37	-0.05118163062940084
1102	38	-0.1976438855086684
1102	39	0.032278524865084535
1102	40	-0.1370496669864636
1102	41	-0.0783759188410258
1102	42	-0.19666451334164492
1102	43	0.124988910188641
1102	44	-0.08901124150441575
1102	45	0.19435100404298566
1102	46	0.2729989486431137
1102	47	0.10808544438922496
1102	48	0.03143321445481462
1102	49	0.005691530532246247
1102	50	-0.16963470571540995
1102	51	0.4648333241665483
1103	1	0.11120262665646408
1103	2	0.17548767399406984
1103	3	0.029742542099562286
1103	4	0.09893575257034826
1103	5	0.16395508783302404
1103	6	0.18768431407320918
1103	7	0.09965021911019534
1103	8	0.22419755545241316
1103	9	-0.023831241971670636
1103	10	0.08509428289642784
1103	11	-0.02611308432516779
1103	12	-0.05195475085834289
1103	13	0.1971367613241526
1103	14	-0.002526812658359745
1103	15	0.07892389305918357
1103	16	-0.23797438723394038
1103	17	-0.024183954043571987
1103	18	-0.058538683289434346
1103	19	0.08664419512324883
1103	20	-0.12126121058297201
1103	21	-0.24947628560169477
1103	22	0.11984818282676457
1103	23	0.03496653533689198
1103	24	-0.09947149811978306
1103	25	-0.09697068577894896
1103	26	0.06466561776270652
1103	27	0.19898971118881548
1103	28	-0.357894541685134
1103	29	-0.2730619158192553
1103	30	0.1407946379383631
1103	31	-0.1538387454892316
1103	32	-0.22381904625260063
1103	33	-0.2854956381341367
1103	34	0.13765948980697829
1103	35	0.0037743316673299887
1103	36	-0.1388722855327984
1103	37	-0.2086887275823628
1103	38	-0.058782052705375065
1103	39	-0.04942300215247286
1103	40	-0.19134883548456116
1103	41	-0.15128109594603245
1103	42	0.06353567267685634
1103	43	0.03577742296307207
1103	44	-0.2176714921001235
1103	45	-0.04255696503030748
1103	46	0.02762729839320599
1103	47	-0.12145441216383546
1103	48	-0.25767326056465123
1103	49	-0.1647009570926059
1103	50	-0.00940400677427533
1103	51	0.1291934722264685
2112	1	0.3547323832203517
2112	2	0.2705275311021893
2112	3	0.11027875563236478
2112	4	0.1153626504579961
2112	5	0.03786463995418643
2112	6	0.10007774473626085
2112	7	0.019812465129092516
2112	8	0.0603168248924959
2112	9	0.09769372637241247
2112	10	0.017426556839623686
2112	11	-0.06995575231118067
2112	12	-0.27883512921994935
2112	13	0.08204027576008599
2112	14	-0.06200295122323496
2112	15	-0.1357275228618562
2112	16	-0.3407557643293089
2112	17	0.11820547665302428
2112	18	-0.1356902208027435
2112	19	0.09436297316284481
2112	20	-0.27323760491090354
2112	21	0.08735882689212104
2112	22	0.016213521482207227
2112	23	-0.20040541961527225
2112	24	-0.5406266330872
2112	25	-0.03613418126326451
2112	26	-0.08006271994515402
2112	27	0.1691678983738285
2112	28	-0.8959005542133269
2112	29	-0.02743216009393734
2112	30	0.04570894473663145
2112	31	-0.32391378888509337
2112	32	-0.36481784710122866
2112	33	0.059752946783390826
2112	34	0.1734236259567794
2112	35	-0.1316834758143699
2112	36	-0.3502650117992445
2112	37	0.03165562173126801
2112	38	-0.0661580495523637
2112	39	-0.251007718423196
2112	40	-0.2377306519892457
2112	41	0.01451854514712636
2112	42	-0.040477140632887654
2112	43	-0.0875100714814181
2112	44	-0.5093411804046255
2112	45	0.021448393415319345
2112	46	-0.1863569113828092
2112	47	-0.39092955283351216
2112	48	-0.3833808940436114
2112	49	0.07723106024896254
2112	50	-0.02581633874633127
2112	51	-0.08066547235261368
1113	1	0.16277044618385617
1113	2	0.018401178434508836
1113	3	0.13825721117645884
1113	4	0.0659349537174947
1113	5	0.09612464683007005
1113	6	0.18275120543727752
1113	7	0.11465482716108255
1113	8	0.08960243627373643
1113	9	0.11807023047339271
1113	10	0.14299917115129546
1113	11	0.05707477991546028
1113	12	0.1348985773759921
1113	13	0.14595401608063696
1113	14	0.10740812878265871
1113	15	0.02526437341042964
1113	16	0.020415199768720527
1113	17	0.1533002493393738
1113	18	-0.12058726253285101
1113	19	0.18258431134204045
1113	20	0.03217832096044759
1113	21	0.15636833854020438
1113	22	0.10943620209710758
1113	23	-0.010818251336040419
1113	24	0.0989638094254488
1113	25	0.17207330690857248
1113	26	0.04605529979847489
1113	27	0.29283688974621924
1113	28	0.05664622639666561
1113	29	0.25408967085739675
1113	30	0.13356992078561092
1113	31	-0.309284071942578
1113	32	0.05939320998503206
1113	33	0.11538893388291172
1113	34	0.07889328199856038
1113	35	-0.17427295242815433
1113	36	-0.0023875008714924206
1113	37	0.18076859915543508
1113	38	0.050177673971369074
1113	39	-0.3949167111052816
1113	40	-0.03185546576721521
1113	41	-0.00769563883205393
1113	42	0.0448159196293787
1113	43	-0.00710666955289696
1113	44	0.15798632479322094
1113	45	0.26107154551983314
1113	46	0.12843972597946873
1113	47	-0.24289425659724628
1113	48	0.0059404064819048585
1113	49	0.19754350397307974
1113	50	0.07368386589325837
1113	51	-0.03489926898997453
2113	1	0.23519251802225227
2113	2	0.08210339827299151
2113	3	-0.022138143923776
2113	4	0.011887062754560418
2113	5	0.05027562137782127
2113	6	0.11156763459324281
2113	7	0.05697439181000874
2113	8	-0.13982241561382835
2113	9	0.07620893268508411
2113	10	0.09893272156097765
2113	11	-0.05049068573219273
2113	12	0.09565870605869264
2113	13	0.07320302440385262
2113	14	0.08783051910391038
2113	15	0.10258712451608432
2113	16	-0.0018923745545222914
2113	17	0.15231594845970164
2113	18	0.04836928930740214
2113	19	0.013477554783754897
2113	20	0.1456874320774233
2113	21	0.20626528752009676
2113	22	0.10975634574654691
2113	23	0.1941536795134482
2113	24	0.22585421517845816
2113	25	0.2175380199532455
2113	26	0.06332327380793146
2113	27	0.1262323402610548
2113	28	0.19745223224072816
2113	29	0.34518792867805104
2113	30	0.22164384527912517
2113	31	0.135280194346862
2113	32	0.23887835543041547
2113	33	0.35557129299414214
2113	34	0.25646702559511425
2113	35	0.12517284507992343
2113	36	0.15761794069130275
2113	37	0.2855135865512608
2113	38	0.185187503628633
2113	39	0.06442167496822293
2113	40	0.22582746078406046
2113	41	0.10915786784973847
2113	42	0.1812072256337629
2113	43	0.1624128284035038
2113	44	0.26400519473921946
2113	45	0.3910441520870265
2113	46	0.1605398500835733
2113	47	0.24955869309776801
2113	48	0.11925152417108364
2113	49	0.2944010421657808
2113	50	0.23180670909190582
2113	51	0.47477378459296915
1112	1	0.2905818093946806
1112	2	0.08262817634821573
1112	3	0.06762736909222866
1112	4	0.04001715650820614
1112	5	0.10620135002034914
1112	6	-0.09298139460732813
1112	7	0.020418700865540984
1112	8	-0.42820277055630385
1112	9	-0.1955306649126598
1112	10	-0.008059709106817081
1112	11	-0.15907894012210733
1112	12	-0.11938245547144533
1112	13	-0.10181795071634525
1112	14	0.11146599323306816
1112	15	0.08571947009461667
1112	16	-0.19218049383494673
1112	17	0.06962528061644946
1112	18	0.0682434157250782
1112	19	-0.15311439397065385
1112	20	-0.09642855176430487
1112	21	0.18993212716959107
1112	22	0.07278023795157651
1112	23	-0.21386796086668625
1112	24	0.15474478563314165
1112	25	0.21043356956997883
1112	26	-0.03230395658462015
1112	27	0.01853852900741515
1112	28	0.031448272686702694
1112	29	0.2378697263887375
1112	30	0.08866867021974656
1112	31	0.14796436961885462
1112	32	0.12437774618032904
1112	33	0.2097763759458786
1112	34	0.18220401244766293
1112	35	-5.211627797407914E-4
1112	36	0.1099145734700997
1112	37	0.19834864552164078
1112	38	0.16913370673181305
1112	39	-0.24710663972945213
1112	40	-0.1709608882510878
1112	41	0.08593298026952567
1112	42	0.16624333484612208
1112	43	-0.47332534722968944
1112	44	-0.18350648160152616
1112	45	0.08239588713525592
1112	46	0.0787598568047514
1112	47	-0.1145777377712065
1112	48	-0.21392620082280445
1112	49	0.156832830160468
1112	50	0.10917404002786668
1112	51	-0.153250347654307
2114	1	0.2801718615010884
2114	2	-0.05165177316330727
2114	3	-0.005650593524499925
2114	4	0.031369833076063
2114	5	0.08673432440870954
2114	6	-0.11358001983710864
2114	7	-0.13040076068262607
2114	8	-0.664915891932786
2114	9	-0.13558246161629484
2114	10	-7.585914651144776E-4
2114	11	-0.09917532821023656
2114	12	-0.08920735207699693
2114	13	-0.2120252656297481
2114	14	0.1019370575917975
2114	15	0.14768493898048263
2114	16	-0.0072123176520833
2114	17	0.11259425217988905
2114	18	-0.005944681371801153
2114	19	-0.0396703607146236
2114	20	0.04862584372521184
2114	21	0.016604880533215294
2114	22	0.14911371573937515
2114	23	0.0059327347783693085
2114	24	0.19424855259980234
2114	25	0.1883417977765518
2114	26	0.11480645846128112
2114	27	0.15516226654829524
2114	28	0.226159796102543
2114	29	0.059855487435406474
2114	30	0.16366836294456988
2114	31	0.18438002916612817
2114	32	0.16545904282514812
2114	33	0.17327665758506328
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2108	2	-0.21165916342933144
2108	3	-0.05992465781243084
2108	4	-0.03382021265590396
2108	5	-0.19163657913324528
2108	6	0.028827657658588635
2108	7	-0.2357354275912158
2108	8	0.036496204899464146
2108	9	0.06047533556781559
2108	10	0.04301833670726296
2108	11	-0.1880776610537822
2108	12	0.11864031487618121
2108	13	-0.2582972373015622
2108	14	0.12905208186628414
2108	15	-0.06174075866819351
2108	16	0.06855179401273404
2108	17	-0.4210393857642458
2108	18	0.00725927414674743
2108	19	-0.011910799041963571
2108	20	-0.07730343563191155
2108	21	-0.3076443079041198
2108	22	0.010806694592623955
2108	23	-0.007872852565462551
2108	24	0.03917517812840288
2108	25	-0.6054459193336186
2108	26	-0.010703959931220197
2108	27	0.0039819004636925115
2108	28	0.04056931691171428
2108	29	-1.003790223486483
2108	30	-0.8103537511881314
2108	31	-0.2994801420721416
2108	32	-0.1306631971534742
2108	33	-0.32336060250257503
2108	34	-1.4282792650219704
2108	35	-0.44578692409022835
2108	36	-0.12148618908938857
2108	37	-0.14582051898624826
2108	38	-0.21628169984842316
2108	39	-0.11824596293730139
2108	40	-0.10130153321529889
2108	41	-1.0456636213453834
2108	42	-0.7013193558888915
2108	43	-0.11903323414251332
2108	44	-0.26704163320852103
2108	45	-0.41822872257213817
2108	46	-0.15446871911530735
2108	47	-0.05984978968478316
2108	48	-0.2347110925807101
2108	49	-0.5359124437566081
2108	50	-0.22866308204135066
2108	51	0.3980245311304955
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	1.0
17	4.0
18	4.0
19	10.0
20	27.0
21	59.0
22	252.0
23	775.0
24	2106.0
25	4777.0
26	8903.0
27	14880.0
28	23046.0
29	34218.0
30	47226.0
31	62476.0
32	82790.0
33	108872.0
34	150365.0
35	227399.0
36	457033.0
37	1888702.0
38	16597.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	17.78064488925489	36.41188274671125	40.7164044846195	5.09106787941436
2	22.61939063197767	37.09589007839587	31.48864630243774	8.796072987188717
3	17.333179578357857	45.82497743187877	20.61598672681425	16.22585626294912
4	10.286974504571441	49.318771757553534	10.953189276420993	29.44106446145403
5	8.221152893990205	53.634857062176856	16.237739265208806	21.90625077862414
6	20.59876915095949	49.63552404359401	22.726465426532698	7.039241378913805
7	35.74490132955462	38.80768127488003	18.470817326950588	6.976600068614755
8	30.087474229537435	31.73697549482163	29.609279219248418	8.566271056392512
9	36.39022501678634	23.830722160713133	30.168706688533092	9.610346133967434
10	41.96574884316418	24.78519556802348	24.673393127408143	8.5756624614042
11	34.70120957463324	33.63870306613402	13.52825503222785	18.13183232700489
12	30.116031767226044	40.08047220239947	12.76898868623188	17.034507344142607
13	25.202921429716834	38.72389333152746	30.27297045029551	5.800214788460199
14	37.51153960904923	14.002872364417179	38.77228781653667	9.713300209996927
15	29.31843315587624	16.69382933581045	34.59486948183083	19.392868026482486
16	28.869977594790903	11.73689244158003	27.432230152032155	31.960899811596917
17	17.138962767231792	18.248490187898376	44.10925717819584	20.503289866673992
18	29.92615927950674	21.25345229964843	33.0665301186192	15.753858302225634
19	22.888770626751707	28.579994007389182	23.910133837104482	24.62110152875463
20	15.991135024765837	37.477998876864625	28.58746879913318	17.94339729923636
21	17.24012800421144	40.53806362006081	30.987228328055195	11.234580047672562
22	40.01591427883273	27.192302114471644	19.045577702376793	13.746205904318831
23	38.11993654732342	29.388932580572824	9.432739971161359	23.0583909009424
24	33.79822917711487	30.313634595125034	12.932731346401654	22.955404881358444
25	44.80160177759492	26.814186260310578	6.5804361061829315	21.80377585591157
26	48.76384832944793	19.03612241025618	10.543896513105482	21.656132747190405
27	48.15225703572759	14.958815175232756	20.15440236484522	16.734525424194434
28	23.752364621619016	19.56440491394087	24.440332954056863	32.24289751038325
29	29.725777362369598	32.388592062282264	15.450618139722385	22.43501243562575
30	28.608615432186706	30.633900672156273	33.712045467177674	7.045438428479339
31	29.846875377333237	35.77396996411461	26.184099648557012	8.195055009995137
32	27.76444311843201	36.61197717185824	24.591234305333103	11.03234540437665
33	34.57752413175822	30.26983998195828	16.306417907301082	18.84621797898242
34	41.88119425450452	24.80637414463147	18.71403555062063	14.598396050243378
35	36.2670506707827	33.516167591219606	13.275134306674733	16.941647431322952
36	40.11522678965361	36.03050864999511	10.243339609177001	13.610924951174278
37	41.832991430822084	40.50490621052975	5.468257370496039	12.193844988152136
38	40.486474779605444	43.54695478900963	7.416846136203483	8.549724295181441
39	32.75600682569872	45.61708877944317	7.762635113249483	13.864269281608626
40	42.35702544176338	38.86361443874217	1.8999706758170043	16.879389443677447
41	39.035406874636244	37.23989162190842	1.7655841421973715	21.959117361257963
42	34.30609974949865	42.08116090543366	2.1450735692002803	21.467665775867413
43	20.04803032848835	41.81577385496732	3.0552412664724926	35.080954550071844
44	21.36052709420346	43.328396989383876	3.510500804658137	31.800575111754526
45	19.82512820545583	33.37970472655998	6.150667524457583	40.64449954352661
46	24.553093701306043	33.88898081534006	8.611535073064493	32.9463904102894
47	21.46974210690741	20.955003670314408	19.17504492860935	38.40020929416883
48	20.379348875363277	20.124630972087083	19.554055202295338	39.9419649502543
49	18.316082749138964	18.42440334231799	31.004158411919803	32.255355496623245
50	23.946421714972775	19.07279361077801	25.655497709327708	31.325286964921506
51	17.192564051618227	28.995962973587154	26.175474888852403	27.635998085942216
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	30.0
1	18.5
2	7.0
3	9.0
4	11.0
5	12.0
6	13.0
7	12.5
8	12.0
9	11.5
10	11.0
11	11.5
12	12.0
13	19.0
14	26.0
15	23.5
16	21.0
17	20.5
18	20.0
19	26.0
20	32.0
21	32.5
22	33.0
23	43.0
24	53.0
25	86.5
26	175.0
27	230.0
28	245.5
29	261.0
30	414.0
31	567.0
32	845.0
33	1123.0
34	2496.0
35	3869.0
36	13170.0
37	22471.0
38	42254.5
39	62038.0
40	116574.5
41	171111.0
42	503727.5
43	836344.0
44	638450.0
45	440556.0
46	283752.5
47	126949.0
48	130570.0
49	134191.0
50	131390.0
51	128589.0
52	237892.5
53	347196.0
54	403102.0
55	459008.0
56	334317.5
57	209627.0
58	180491.5
59	151356.0
60	89328.0
61	27300.0
62	15797.0
63	4294.0
64	3094.0
65	1894.0
66	1321.5
67	749.0
68	485.0
69	221.0
70	154.5
71	88.0
72	63.5
73	39.0
74	34.5
75	20.5
76	11.0
77	7.5
78	4.0
79	2.5
80	1.0
81	2.0
82	3.0
83	1.5
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	3130522.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	9.691674267168066
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.33188253276458	7.591670902416261
2	9.143654238463196	1.7723463698159192
3	3.595702363615797	1.0454512820955184
4	2.070146034521891	0.802527242082233
5	1.3900963247965181	0.6736180389957659
6	0.9533338306786007	0.5543640572885131
7	0.6932278514248298	0.47029769702567614
8	0.4566664218837369	0.3540689767720263
9	0.36908929192363865	0.32193838735412494
>10	2.259247763998249	4.371431302109314
>50	0.35300489756105835	2.4252078991476056
>100	0.304040925450942	6.1065885579429855
>500	0.037502840276346726	2.4846546067339252
>1k	0.028598514220860023	5.826918442563649
>5k	0.003944619548538456	2.8542493938827747
>10k+	0.009861548871346139	62.34466684377379
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TGAAAAAATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGTCT	353327	11.286520267226999	Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GAAAAAATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGTCTG	214770	6.860517191701576	Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTCCAGGGGGCATGCCTGTTTGAGCGTCATTAGATCGGAAGAGCACACGTC	192917	6.162454696053885	No Hit
AAAAAATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGTCTGA	174578	5.57664185078399	Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATTAGATCGGAAGAGCACACGT	140019	4.472704552148172	No Hit
TAGCCTGTGGGAATACTGCCAGCTGGGACTGAGGAAGATCGGAAGAGCACA	127134	4.061111852911432	No Hit
AAAAATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGTCTGAA	90299	2.8844710243211837	Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTGAAAAAATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGTC	82271	2.6280281691040663	No Hit
ACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACAC	73220	2.33890705767281	No Hit
AAAATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGTCTGAAC	69069	2.2063093631030224	Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GGAATACTGCCAGCTGGGACTGAGGACTGCGACGAGATCGGAAGAGCACAC	60786	1.9417209014982166	No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTAGATCGGAAGAGCACACG	55150	1.7616870285530655	No Hit
AATACTGCCAGCTGGGACTGAGGACTGCGACGAGATCGGAAGAGCACACGT	33855	1.0814490362949054	No Hit
TTCCAGGGGGCATGCCTGTTTGAGCGTCATTTAGATCGGAAGAGCACACGT	25552	0.8162217036008692	No Hit
GGGAATACTGCCAGCTGGGACTGAGGACTGCGACGAGATCGGAAGAGCACA	22134	0.707038634451379	No Hit
TAGCCTGTGGGAATACTGCCAGCTGGGACTGAGGAGATCGGAAGAGCACAC	21615	0.690459929685848	No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCAGATCGGAAGAGCACACGTCTG	21321	0.6810685246741598	Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GAATACTGCCAGCTGGGACTGAGGACTGCGACGAGATCGGAAGAGCACACG	20193	0.6450361952415604	No Hit
TACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACA	19180	0.6126773745720362	No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATTAGATCGGAAGAGCACACGTCT	18913	0.6041484455308093	Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTCCAGGGGGCATGCCTGTTTGAGCGTCAGATCGGAAGAGCACACGTCTGA	18743	0.5987180412723501	Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTTGAAAAAATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGT	17287	0.5522082259763708	No Hit
CTTTGAAAAAATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACG	16889	0.5394946913006841	No Hit
AAATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGTCTGAACT	15834	0.5057942413437759	Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ACTGCCAGCTGGGACTGAGGACTGCGACGAGATCGGAAGAGCACACGTCTG	13584	0.4339212438053462	Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGCCTGTGGGAATACTGCCAGCTGGGACTGAGGAAGATCGGAAGAGCACAC	13027	0.4161286839702772	No Hit
CCAGGGGGCATGCCTGTTTGAGCGTCATTAGATCGGAAGAGCACACGTCTG	11864	0.3789783301315244	Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATTCCAGGGGGCATGCCTGTTTGAGCGTAGATCGGAAGAGCACACGTCTGA	11750	0.3753367649229106	Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCAC	10851	0.34661950946200026	No Hit
AGGGGGCATGCCTGTTTGAGCGTCATTAGATCGGAAGAGCACACGTCTGAA	10804	0.34511816240230864	Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGTCTGAACTCC	9699	0.30982053472232424	Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGTCTGAACTC	9277	0.296340354739561	Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GAAAAAATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGTCTA	8840	0.28238102143987487	Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
CTATGTTCCTTGGAACAGGACGTCAGATCGGAAGAGCACACGTCTGAACTC	8791	0.28081578727126016	Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TTCCAGGGGGCATGCCTGTTTGAGCGTAGATCGGAAGAGCACACGTCTGAA	8369	0.2673356072884969	Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGTCTGAACTCCA	7799	0.24912778124542806	Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGAATACTGCCAGCTGGGACTGAGGACTGCGACGTAGATCGGAAGAGCACA	7410	0.23670173855989513	No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAATAGATCGGAAGAGCACACG	7152	0.22846030150882185	No Hit
AATACTGCCAGCTGGGACTGAGGACTGCGACGTAGATCGGAAGAGCACACG	6920	0.22104939687374822	No Hit
AGAGGTGCACAATCGACCGATCCTGAAGATCGGAAGAGCACACGTCTGAAC	5161	0.16486068457592695	Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CTATGTTCCTTGGAACAGGACGTAGATCGGAAGAGCACACGTCTGAACTCC	5103	0.16300795841715854	Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GAATACTGCCAGCTGGGACTGAGGACTGCGACGTAGATCGGAAGAGCACAC	5071	0.16198576467438977	No Hit
CTATGTTCCTTGGAACAGGACGTCATAGATCGGAAGAGCACACGTCTGAAC	4727	0.1509971819396254	Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ATGCCTGTTTGAGCGTCATTTCCTTCTCAAACAGATCGGAAGAGCACACGT	4622	0.14764310872116534	No Hit
TTGATGATTCATAATAACTTTTCGAATCGCAAGATCGGAAGAGCACACGTC	4510	0.14406543062147462	No Hit
TTCCAGGGGGCATGCCTGTTTGAGCGTCATAGATCGGAAGAGCACACGTCT	4231	0.13515317892670936	Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGAGGTGCACAATCGACCGATCCTGAGATCGGAAGAGCACACGTCTGAACT	4230	0.13512123537224782	Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TAGCCTGTGGGAATACTGCCAGCTGGGACTGAGGAAGATCGGAAGAGCACC	4038	0.12898807291563516	No Hit
ATACTGCCAGCTGGGACTGAGGACTGCGACGAGATCGGAAGAGCACACGTC	3895	0.12442014462763719	No Hit
CAGGGGGCATGCCTGTTTGAGCGTCATTAGATCGGAAGAGCACACGTCTGA	3867	0.1235257251027145	Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTACAACGGGGTATTGTAAGCAGTAGAGTAGATCGGAAGAGCACACGTCTG	3784	0.12087441008240798	Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCAGGGGGCATGCCTGTTTGAGCGTCATTTAGATCGGAAGAGCACACGTCT	3775	0.12058691809225426	Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGGGAATACTGCCAGCTGGGACTGAGGACTGCGACGAGATCGGAAGAGCAC	3664	0.11704118354702506	No Hit
ATCGAATCTTTGAACGCACATTGCGCCCCTAGATCGGAAGAGCACACGTCT	3536	0.11295240857594996	Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAAAAATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGTCTGG	3476	0.11103579530825851	Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
AGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGTCTGAACTCCAGT	3450	0.11020526289225885	Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TAGCCTGTGGGAATACTGCCAGCTGGGACTAGATCGGAAGAGCACACGTCT	3401	0.10864002872364416	Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAAAAATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGTCTGC	3270	0.1044554230891845	Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATAGATCGGAAGAGCACACGTC	3251	0.10384849555441553	No Hit
GGGAATACTGCCAGCTGGGACTGAGGACTGCGACGTAGATCGGAAGAGCAC	3225	0.1030179631384159	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA Adapter	Nextera Transposase Sequence
1	0.011851058705225518	0.0	0.0
2	0.013735728418455452	0.0	0.0
3	0.015173188369224046	0.0	0.0
4	0.01638704343876197	0.0	0.0
5	0.01712174519137703	0.0	0.0
6	0.018207826043068854	0.0	0.0
7	0.019485568221529827	0.0	0.0
8	0.020060552201837265	0.0	0.0
9	0.020571649073221655	0.0	0.0
10	0.021146633053529092	0.0	0.0
11	0.0219452219150672	0.0	0.0
12	0.0253631822424503	0.0	0.0
13	0.027152021292295662	0.0	0.0
14	0.030889417164294004	0.0	0.0
15	0.0333810144122929	0.0	0.0
16	0.03715035383875277	0.0	0.0
17	0.04159050790890465	0.0	0.0
18	0.04644592818705635	0.0	0.0
19	0.05717896248612851	0.0	0.0
20	0.07506735298458213	0.0	0.0
21	0.24794586973035168	0.0	0.0
22	0.5619510100871357	0.0	0.0
23	1.4313267883119811	0.0	0.0
24	2.6600675542289753	0.0	0.0
25	4.340490180231923	0.0	0.0
26	6.0407497535554775	0.0	0.0
27	9.849858905319943	0.0	0.0
28	15.775516032150549	0.0	0.0
29	29.815730411733252	0.0	0.0
30	41.36466697886167	0.0	0.0
31	55.814972710621426	0.0	0.0
32	66.91465512780297	0.0	0.0
33	75.56998481403421	0.0	0.0
34	80.16014581593741	0.0	0.0
35	86.81149661302491	0.0	0.0
36	93.68076633864895	0.0	0.0
37	94.74094096767249	0.0	0.0
38	94.97499139121207	0.0	0.0
39	95.07730659615234	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCCTAT	55	1.8189894E-12	45.0	2
TTGAGGG	20	7.0351525E-4	45.0	20
CTACGTC	20	7.0351525E-4	45.0	6
GCGTGTC	25	3.892352E-5	45.0	18
TTGCACT	20	7.0351525E-4	45.0	7
TAGTCTG	20	7.0351525E-4	45.0	1
GCACTCT	20	7.0351525E-4	45.0	9
ATTCCAG	27300	0.0	44.538464	1
TAGCCTG	18515	0.0	44.355923	1
TTGCTCG	1860	0.0	43.54839	1
CGTCTGC	895	0.0	42.988827	45
CGTCTGG	885	0.0	42.711864	45
ATAGCCT	180	0.0	42.5	1
GTAGCCA	80	0.0	42.1875	28
CAGTCTC	205	0.0	41.707317	2
TCAGTCT	200	0.0	41.625	1
TGCTCGA	1970	0.0	41.11675	2
GCGACTG	55	6.184564E-11	40.909092	2
TAATCGC	55	6.184564E-11	40.909092	10
TGGGCCA	855	0.0	40.789474	1
>>END_MODULE