FastQCFastQC Report
Wed 5 Aug 2015
000000000-AH0J7 l01n01 150804_gms5.3410000000ee70.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AH0J7 l01n01 150804_gms5.3410000000ee70.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3130522
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAAAAAATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGTCT35332711.286520267226999Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GAAAAAATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGTCTG2147706.860517191701576Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTCCAGGGGGCATGCCTGTTTGAGCGTCATTAGATCGGAAGAGCACACGTC1929176.162454696053885No Hit
AAAAAATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGTCTGA1745785.57664185078399Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATTAGATCGGAAGAGCACACGT1400194.472704552148172No Hit
TAGCCTGTGGGAATACTGCCAGCTGGGACTGAGGAAGATCGGAAGAGCACA1271344.061111852911432No Hit
AAAAATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGTCTGAA902992.8844710243211837Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTGAAAAAATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGTC822712.6280281691040663No Hit
ACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACAC732202.33890705767281No Hit
AAAATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGTCTGAAC690692.2063093631030224Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GGAATACTGCCAGCTGGGACTGAGGACTGCGACGAGATCGGAAGAGCACAC607861.9417209014982166No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTAGATCGGAAGAGCACACG551501.7616870285530655No Hit
AATACTGCCAGCTGGGACTGAGGACTGCGACGAGATCGGAAGAGCACACGT338551.0814490362949054No Hit
TTCCAGGGGGCATGCCTGTTTGAGCGTCATTTAGATCGGAAGAGCACACGT255520.8162217036008692No Hit
GGGAATACTGCCAGCTGGGACTGAGGACTGCGACGAGATCGGAAGAGCACA221340.707038634451379No Hit
TAGCCTGTGGGAATACTGCCAGCTGGGACTGAGGAGATCGGAAGAGCACAC216150.690459929685848No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCAGATCGGAAGAGCACACGTCTG213210.6810685246741598Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GAATACTGCCAGCTGGGACTGAGGACTGCGACGAGATCGGAAGAGCACACG201930.6450361952415604No Hit
TACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACA191800.6126773745720362No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATTAGATCGGAAGAGCACACGTCT189130.6041484455308093Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTCCAGGGGGCATGCCTGTTTGAGCGTCAGATCGGAAGAGCACACGTCTGA187430.5987180412723501Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTTGAAAAAATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGT172870.5522082259763708No Hit
CTTTGAAAAAATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACG168890.5394946913006841No Hit
AAATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGTCTGAACT158340.5057942413437759Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ACTGCCAGCTGGGACTGAGGACTGCGACGAGATCGGAAGAGCACACGTCTG135840.4339212438053462Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGCCTGTGGGAATACTGCCAGCTGGGACTGAGGAAGATCGGAAGAGCACAC130270.4161286839702772No Hit
CCAGGGGGCATGCCTGTTTGAGCGTCATTAGATCGGAAGAGCACACGTCTG118640.3789783301315244Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATTCCAGGGGGCATGCCTGTTTGAGCGTAGATCGGAAGAGCACACGTCTGA117500.3753367649229106Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCAC108510.34661950946200026No Hit
AGGGGGCATGCCTGTTTGAGCGTCATTAGATCGGAAGAGCACACGTCTGAA108040.34511816240230864Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGTCTGAACTCC96990.30982053472232424Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGTCTGAACTC92770.296340354739561Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GAAAAAATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGTCTA88400.28238102143987487Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
CTATGTTCCTTGGAACAGGACGTCAGATCGGAAGAGCACACGTCTGAACTC87910.28081578727126016Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TTCCAGGGGGCATGCCTGTTTGAGCGTAGATCGGAAGAGCACACGTCTGAA83690.2673356072884969Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGTCTGAACTCCA77990.24912778124542806Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGAATACTGCCAGCTGGGACTGAGGACTGCGACGTAGATCGGAAGAGCACA74100.23670173855989513No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAATAGATCGGAAGAGCACACG71520.22846030150882185No Hit
AATACTGCCAGCTGGGACTGAGGACTGCGACGTAGATCGGAAGAGCACACG69200.22104939687374822No Hit
AGAGGTGCACAATCGACCGATCCTGAAGATCGGAAGAGCACACGTCTGAAC51610.16486068457592695Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CTATGTTCCTTGGAACAGGACGTAGATCGGAAGAGCACACGTCTGAACTCC51030.16300795841715854Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GAATACTGCCAGCTGGGACTGAGGACTGCGACGTAGATCGGAAGAGCACAC50710.16198576467438977No Hit
CTATGTTCCTTGGAACAGGACGTCATAGATCGGAAGAGCACACGTCTGAAC47270.1509971819396254Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ATGCCTGTTTGAGCGTCATTTCCTTCTCAAACAGATCGGAAGAGCACACGT46220.14764310872116534No Hit
TTGATGATTCATAATAACTTTTCGAATCGCAAGATCGGAAGAGCACACGTC45100.14406543062147462No Hit
TTCCAGGGGGCATGCCTGTTTGAGCGTCATAGATCGGAAGAGCACACGTCT42310.13515317892670936Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGAGGTGCACAATCGACCGATCCTGAGATCGGAAGAGCACACGTCTGAACT42300.13512123537224782Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TAGCCTGTGGGAATACTGCCAGCTGGGACTGAGGAAGATCGGAAGAGCACC40380.12898807291563516No Hit
ATACTGCCAGCTGGGACTGAGGACTGCGACGAGATCGGAAGAGCACACGTC38950.12442014462763719No Hit
CAGGGGGCATGCCTGTTTGAGCGTCATTAGATCGGAAGAGCACACGTCTGA38670.1235257251027145Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTACAACGGGGTATTGTAAGCAGTAGAGTAGATCGGAAGAGCACACGTCTG37840.12087441008240798Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCAGGGGGCATGCCTGTTTGAGCGTCATTTAGATCGGAAGAGCACACGTCT37750.12058691809225426Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGGGAATACTGCCAGCTGGGACTGAGGACTGCGACGAGATCGGAAGAGCAC36640.11704118354702506No Hit
ATCGAATCTTTGAACGCACATTGCGCCCCTAGATCGGAAGAGCACACGTCT35360.11295240857594996Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAAAAATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGTCTGG34760.11103579530825851Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
AGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGTCTGAACTCCAGT34500.11020526289225885Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TAGCCTGTGGGAATACTGCCAGCTGGGACTAGATCGGAAGAGCACACGTCT34010.10864002872364416Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAAAAATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGTCTGC32700.1044554230891845Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATAGATCGGAAGAGCACACGTC32510.10384849555441553No Hit
GGGAATACTGCCAGCTGGGACTGAGGACTGCGACGTAGATCGGAAGAGCAC32250.1030179631384159No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTAT551.8189894E-1245.02
TTGAGGG207.0351525E-445.020
CTACGTC207.0351525E-445.06
GCGTGTC253.892352E-545.018
TTGCACT207.0351525E-445.07
TAGTCTG207.0351525E-445.01
GCACTCT207.0351525E-445.09
ATTCCAG273000.044.5384641
TAGCCTG185150.044.3559231
TTGCTCG18600.043.548391
CGTCTGC8950.042.98882745
CGTCTGG8850.042.71186445
ATAGCCT1800.042.51
GTAGCCA800.042.187528
CAGTCTC2050.041.7073172
TCAGTCT2000.041.6251
TGCTCGA19700.041.116752
GCGACTG556.184564E-1140.9090922
TAATCGC556.184564E-1140.90909210
TGGGCCA8550.040.7894741
Produced by FastQC (version 0.11.2)