FastQCFastQC Report
Wed 5 Aug 2015
000000000-AH0J7 l01n01 150804_gms4.3410000000ee63.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AH0J7 l01n01 150804_gms4.3410000000ee63.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2092141
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGAGACAACAAAGGCTTAATCTCAGCAGATCGGAAGAGCACACGTCTGAA143180.6843706996803753Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGC32860.15706398373723376TruSeq Adapter, Index 11 (100% over 50bp)
GTCGACGTACTTCATAGGATCATTTCTATAAGATCGGAAGAGCACACGTCT26410.1262343216829076Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGACGT11050.038.280542
GCACACA5400.037.91666845
GCTGTAA5450.036.3302733
TCATAGG12100.034.95867512
CTGTAAT5750.034.4347844
AACGGCT5100.033.9705914
TGTAACG5100.033.9705911
ATAGGAT12400.033.9314514
CATAGGA12500.033.8413
GGGAGAC22550.033.8248331
CGACGTA12500.033.663
GGCTGTA5950.033.655462
TATAGTG4750.033.6315776
CGTCTGG3700.033.4459545
TAGGATC12650.033.26086815
GGATCAT12900.032.61627617
CGATATA4350.032.5862053
ACGTCTA9600.032.57812545
TGTAATG6200.031.9354845
TAACGGC5450.031.37614413
Produced by FastQC (version 0.11.2)