FastQCFastQC Report
Wed 5 Aug 2015
000000000-AH0J7 l01n01 150804_gms3.3410000000ee57.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AH0J7 l01n01 150804_gms3.3410000000ee57.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2363218
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGAGACAACAAAGGCTTAATCTCAGCAGATCGGAAGAGCACACGTCTGAA80340.33996017295061226Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACA6250.038.5245
TCGACGT11700.035.192312
TCATAGG11750.034.85106712
CATAGGA12150.033.70370513
ATAGGAT12100.033.65702414
CGACGTA12250.033.6122473
GGGAGAC13650.033.461541
GCTGTAA6200.033.3870963
GGATCAT12600.031.96428717
GGCTGTA6550.031.9465662
TTCATAG13150.031.48289111
TAGGATC13050.031.03448115
CTGTAAT6850.030.5474434
CTTCATA14000.029.73214110
GAGCACC6000.029.62500245
CTATAGG7100.029.47183226
ACGTCTA9100.029.17582345
CGTCTGG5450.028.8990845
TGTAATG7400.028.8851365
AGGCTTA15800.028.76582113
Produced by FastQC (version 0.11.2)