FastQCFastQC Report
Wed 5 Aug 2015
000000000-AH0J7 l01n01 150804_gms2.3410000000ee4a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AH0J7 l01n01 150804_gms2.3410000000ee4a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1912363
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC246451.2887197671153436TruSeq Adapter, Index 9 (100% over 50bp)
AAACTTTCAACAACGGATCTCTTGGTTCTCGCATCGATGAAAGATCGGAAG20250.10588993825962958No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGACG9700.039.201031
GCTGTAA6000.037.8750043
CGTATGC33700.037.4554945
TCATAGG10150.037.01970312
GACGTAC10400.036.3461574
TCGACGT10600.036.0849082
GCACACA5100.035.73529445
ATAGGAT10600.035.44811214
GGATCAT10750.035.1627917
CATAGGA10700.035.11682513
CTGTAAT6500.034.961544
CGACGTA10900.034.6788983
ACGTACT11150.034.1031385
GTAATGG6700.033.9179086
TGCGAAC401.561107E-533.757
GGCTGTA6800.033.4191172
CGTACTT11500.033.0652166
CGGCTCG551.1283737E-732.72727226
TCGTATG38900.032.56426644
TTCATAG11750.032.1702111
Produced by FastQC (version 0.11.2)