FastQCFastQC Report
Wed 5 Aug 2015
000000000-AH0J7 l01n01 150804_gms1.3410000000ee3d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AH0J7 l01n01 150804_gms1.3410000000ee3d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3914794
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC63430.16202640547625238TruSeq Adapter, Index 2 (100% over 50bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGTAC7300.030.8219184
AACGGCT13000.027.69230714
TAACGGC13050.027.58620813
TAGTGTA13850.027.292428
CGGCTCG1004.5474735E-1127.026
TATAGTG10900.026.628446
CGATATA10750.026.5813963
TCATAGG8300.026.56626312
CCGCGCG856.491973E-926.47058718
CATAGGA8450.026.09467313
GCTGTAA8500.025.9411753
GTCGACG9050.025.3591141
ACGTACT9050.025.3591145
ACGGCTA14400.025.312515
GGTCCCG1251.8189894E-1225.28
TGACACG1750.024.42857225
CTATCAC15100.024.13907219
TCGACGT9800.024.1071432
GAGCACC3000.024.00000245
TCGGAAA15250.023.9016445
Produced by FastQC (version 0.11.2)