FastQCFastQC Report
Mon 20 Jul 2015
000000000-AH092 l01n03.3330000000e715.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AH092 l01n03.3330000000e715.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16310244
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAAACCTTGTTACGACTTTTACTTCCTCTAAATGACCAAGTTTGATCAA9174285.624857604827984No Hit
AGATACCATGTTACGACTTTCTCATTCTTCAGATGATTTGGTTTACAAGA8856165.429814538642095No Hit
GGAAACCTTGTTACGACTTTTACTTCCTCTAAATGACCAAGTTTGGATAA7646154.687943356334828No Hit
GGAAACCTTGTTACGACTTTTACTTCCTCTAAATGTTCAAGTTTGGGCAT5402413.3122803067814317No Hit
GGAAACCTTGTTACGACTTTTACTTCCTCTAAATGACCAAGTTTGGATGA3607912.2120515180520903No Hit
CAAGCAGAAGACGGGCGGTGTGTACCGAACGGCGATAGTGTAGATCTCGG3582592.1965275320222064No Hit
GGAAACCTTGTTACGACTTTTACTTCCTCTAAATGACCAAGTTTGACCAA2920181.7903962687498727No Hit
GGAAACCTTGTTACGACTTTTACTTCCTCTAGATGATCAAGTTTGATCGT2851151.7480731741352247No Hit
GAAGACCTTGTTACGACTTCTCCTTCCTACAAACAAAAAGGTTCAGTAGG2782721.706117946488109No Hit
GGAAACCTTGTTACGACTTTTACTTCCTCTAGAAAATTCGATTTGAGCAT2328821.4278265855495478No Hit
GGAAACCTTGTTACGACTTTTACTTCCTCTAAATGATCAAGTTTGATCTC1945511.1928147733412204No Hit
GGAAACCTTGTTACGACTTTTACTTCCTCTAAATGATCAAGTTTGATTCA1885301.1558993231493042No Hit
GGAAACCTTGTTACGACTTTTGCCCGGTTCAAGCCACTGCGATTAAAGCG1852041.1355072309157361No Hit
GGAAACCTTGTTACGACTTCACCTTCCTCTAAATGATGAGGTTTAAATAA1685131.0331727716642374No Hit
GGAAACCTTGTTACGACTTTTACTTCCTCTAAATGTTCAAGTTTGGGTAT1492760.9152284907570971No Hit
GGAAACCTTGTTACGACTTTTACTTCCTCTAAATGACCAAGTTTGATCAG1472090.9025554737255923No Hit
GGAAACCTTGTTACGACTTTTACTTCCTCTAAATGATCAAGTTTGATCTT1416950.8687484994093282No Hit
GGAAACCTTGTTACGACTTCTCCTTCCTCTAAGTGATAAGGTTTACTTAA1387570.8507352802324724No Hit
GGAAACCTTGTTACGACTTTTACTTCCTCTAAACGACCAAGTTTGACTAA1300250.7971983742241993No Hit
GGAAACCTTGTTACGACTTTTACTTCCTCTAAATGACCGAGTTTGACGAA1150250.705231632341No Hit
GGAAACCTTGTTACGACTTCTCCTTCCTCTAAGTGATATGGTTTACTTGA1097660.6729880926367502No Hit
GGAAACCTTGTTACGACTTTTACTTCCTCTAAATGGTCAAGTTCGGTCAT1088330.6672677612916152No Hit
GGAAACCTTGTTACGACTTTTACTTCCTCTAGATAACGTAGTTCGATCAA992730.6086542911313896No Hit
GGATACCGTGTTACGACTTCTTCATCCTCTAGATGATAAGGCTCAGGGGA937740.5749392835570087No Hit
AGATACCATGTTACGACTTTCTCATTCTTCAGATGATTTGGTTCACAAGA887620.5442101295357691No Hit
GGAAACCTTGTTACGACTTTTACTTCCTCTAAGTAATCAAGTTTGGTCAT867190.5316842592912773No Hit
GGAAACCTTGTTACGACTTTTACTTCCTCTAAATGACCAAGTTTGTCCAA859610.527036873268113No Hit
GGAAACCTTGTTACGACTTCTCCTTCCTCTAAATGATAAGGTTCAAGCAA846320.5188886199372615No Hit
GGAAACCTTGTTACGACTTCTCCTTCCTCTAAATGGTAAGGTTTGAACAA778840.4775158483220729No Hit
GGATACCTTGTTACGACTTTTACTTCCTCTAAATGACCAAGTTTGACCAA760820.46646757706383796No Hit
GGAAACCTTGTTACGACTTTTACTTCCTCTAAATGACCGAGTTTGACCAA731740.44863829137074834No Hit
GGAAACCTTGTTACGACTTCTCCTTCCTCTAGTTAAAAAGATTTGAACAA656100.40226252899711373No Hit
GGAAACCTTGTTACGACTTTTACTTCCTCTAAATGATCAAGTTTGATCCA611820.37511394679319326No Hit
GGAAACCTTGTTACGACTTCTCCTTCCTCTAAATGTTAAGGTTTACACAA590770.36220794734891765No Hit
GGAAACCTTGTTACGACTTCTCCTTCCTCTAAATGATAAGGTTCAGTGGA564070.3458378672937082No Hit
GGAAACCTTGTTACGACTTCTCCTTCCTCTAAATGATAAGGTTTAAGCAA549490.3368986999826612No Hit
GGAAACCTTGTTACGACTTTTGCCCGGTTCAAGCCGCTGCGATTAAAGCG516810.31686221248437485No Hit
GGAAACCTTGTTACGACTTCACCTTCCTCTAAATGGCGAGGTTAAAAAAA491140.3011236373900967No Hit
GGAAACCTTGTTACGACTTTTACTTCCTCTAAATGACCAAGTTTGGACAA478420.2933248576784014No Hit
GACTACCTTGTTACGACTTCTCCCCCGTTGCTGACCCCAGGTTCGATAGC454340.2785611300480851No Hit
AAGCAGAAGACGGGCGGTGTGTACCGAACGGCGATAGTGTAGATCTCGGT433300.26566126171993504No Hit
GGAAACCTTGTTACGACTTCTCCTTCCTCTAAATGATAAGGTTTAATCAA416890.255600100157913No Hit
GGAAACCTTGTTACGACTTTTACTTCCTCTAAATGACCAAGTTTGACTAG416800.25554492011278307No Hit
GGCTACCTTGTTACGACTTCACCCCAGTCGCTGACCCTACCGTGGCCGGC383340.23503020555670412No Hit
GGAAACCTTGTTACGACTTCTTCTTCCTCTAGGCGTCCAAATTTACAAAG381290.23377332675096704No Hit
AGATACCTTGTGACGACTTAACCTACCTTGTCATAAAAGGATTTTTCGAA371520.227783226296308No Hit
GGAAACCTTGTTACGACTTCTCCTTCCTCTAAATGATAAGGTTCAGTGAA364700.2236018050986852No Hit
GGAAACCTTGTTACGACTTCTCCTTCCTCTAAATGATAAGGTTAAAGCGA361480.2216275857062592No Hit
GGCTACCTTGTTACGACTTCACCCCAGTCGCTGACCTTACCGTGGCCGGC359530.2204320180617776No Hit
AGCTACCTTGTTACGACTTTTGCTTCCTCTATTGAAGAAATATCGGTGAA343080.21034633203525344No Hit
GGAGACCTTGTTACGACTTCTCCTTCCTACAAACAAGAAGGTTCAGTTAA339450.20812073688167998No Hit
GGAAACCTTGTTACGACTTGTACTTCCTCTAAATGGCCGAGTTCGAAGGT334130.2048589831028892No Hit
GGAAACCTTGTTACGACTTGTACTTCCTCTAAATGACCAAGTTTGACCAA328250.20125388682106776No Hit
GGAAACCTTGTTACGACTTTTACTTCCTCTAGATGATCAAGTTTGTTCTC327500.2007940531116518No Hit
GGTTACCTTGTTACGACTTTTACTTCCTCTAAATGACCAAGTTTGCCCAA323910.19859298242258056No Hit
GGAAACCTTGTTACGACTTTTGCTTCCTCTAAATGAGCAAGTTTGCGTTA321090.1968640076751764No Hit
GGAAACCTTGTTACGACTTTTACTTCCTCTAAATGACCAAGTTTGACTAA315180.19324051804497838No Hit
GGAAACCTTGTTACGACTTTTACTTCCTCTAGAACTAGGCGATAGATCAA301170.18465082435308755No Hit
GGAAACCTTGTTACGACTTTTACTTCCTCTAAATGAGCAAGTTTGATTCA299630.18370663246975336No Hit
GGAAACCTTGTTACGACTTCTCCTTCCTCTAAATGATAAGGTTTACTGAT296120.18155461070968648No Hit
GGAAACCTTGTTACGACTTCACCTTCCTCTAAATGATGAGGTTTAGATAA293990.18024868297494506No Hit
GGAGACCTTGTTACGACTTTTACTTCCTCTAAATGAGCAAGTTTGATTCA293790.18012606065243414No Hit
GGAAACCTTGTTACGACTTTTACTTCCTCTAAATGACCAAGTTTGAACAA289660.1775939096925834No Hit
GGAAACCTTGTTACGACTTTTACTTCCTCTAAGTAATCAAGTTTGGGCAT288600.17694401138327542No Hit
GGAAACCTTGTTACGACTTCTCCTTCCTCTAAATAGTGAGGTTAAATCAG278960.17103361543824852No Hit
GGAAACCTTGTTACGACTTCTTCTTCCTCTAGTCGTCCAAATTTACAAAA276720.16966024542612604No Hit
GGAAACCTTGTTACGACTTTTACTTCCTCTAGATGATCAAGTTTGGGTCA275720.1690471338135714No Hit
GGAAACCTTGTTACGACTTCTCCTTCCTCTAAATGATAAGGTTTACCCAA274010.1679987129561029No Hit
GGTTACCTTGTTACGACTTTTACTTCCTCTAAATGACCAAGTTTGACCAA264750.16232129942384677No Hit
GGAAACCTTGTTACGACTTTTACTTCCTCTGAGCGATCCAGTTTGGCCAT254560.15607369209191474No Hit
GGAAACCTTGTTACGACTTTTGCAATCTCTAGGTACCCAAATTTATTGAA252560.15484746886680542No Hit
AGATACCTTGTTACGACTTAACCCCGCTTGTAGACCCCAAGTTCGAATTC251880.15443055297026825No Hit
GGAAACCTTGTTACGACTTCTCCTTCCTCTAAACAGTAAGGTTTACTCAA250180.15338826322892535No Hit
GGAAACCTTGTTACGACTTTTACTTCCTCTAAATGACCAAGTTTGACCAG244510.1499119203857404No Hit
GAAGACCTTGTTACGACTTCTCGTTCCTACAAATAGAGAAATTCAGTTGA240320.14734298272913637No Hit
GAATACCTTGTTACGACTTTTACTTCCTCTAAATGATCCAGTTTGAGCAT232940.14281821902848296No Hit
GGTTACCTTGTTACGACTTCACCCCAGTCACCAGTCACTCTTTAGGCGGC231810.1421254029062962No Hit
GGAAACCTTGTTACGACTTCTCCTTCCTCTAGGTGATAAGGTTTACTTAT230250.1411689487907109No Hit
GGAAACCTTGTTACGACTTCACCTTCCTCTAAATAACGAGGTTAAAAAAA230240.14116281767458536No Hit
GGAAACCTTGTTACGACTTCTCCTTCCTCTAGGTGGGAGGGTTTAATGAA230080.14106471981657662No Hit
GGAAACCTTGTTACGACTTTTACTTCCTCTAAATGATCAAGTTTGATCAA229300.140586492758784No Hit
GGAAACCTTGTTACGACTTTTACTTCCTCTAAATGAGCAAGTTTGATCCA221680.13591458227111747No Hit
GGAAACCTTGTTACGACTTCTCCTTCCTCTAGATGGTAAGGTTCAATCGG220370.13511140605867086No Hit
GGATACCTTGTTACGACTTAACCCCGCTTGCAGAATCGAAGTTCAAACAC210350.12896802770087312No Hit
GGAAACCTTGTTACGACTTTTGCTTCCTCTAAGCGAGTAGATTCACATCA209790.12862468519784254No Hit
CGTTACCTTGTTACGACTTCTCCTTCCTCTAGATAACGTGATTTAATCAC209560.12848366952695495No Hit
GGAAACCTTGTTACGACTTCTCCTTCCTCTAAATGATAAGGTTCAATGGA206850.12682213705693182No Hit
GGAAACCTTGTTACGACTTTTACTTCCTCTAGATGATCAAGTTTGTCCCA206330.1265033190184034No Hit
GGAAACCTTGTTACGACTTCTACTTCCTCTAAATGATCAAGTTTAAACAA203940.12503798226439777No Hit
GGAAACCTTGTTACGACTTCTCCTTCCTCTAAATGGTAAGGTTTGAACAG203430.12472529534199488No Hit
GGAAACCTTGTTACGACTTTTACTTCCTCTAAATGACCGGGTTTGACCAG201840.12375044787803298No Hit
GGAAACCTTGTTACGACTTTTACTTCCTCTAAATGATCAAGTTTGTCCCA199710.12244452014329155No Hit
GGAAACCTTGTTACGACTTCTCCTTCCTCTAAATGATAAGGTTTGAATAA189720.11631953513387047No Hit
GGAAACCTTGTTACGACTTCTCCTTCCTCTAAACGGTAAGGTTTAGCCTA181620.1113533310721777No Hit
GGAGACCTTGTTACGACTTCTCCTTCCTCTAAATGGTAAGGTTCAATCGA180660.11076474392412523No Hit
GGAAACCTTGTTACGACTTTTACTTCCTCTAGATGACCAAGTTTGATCAA179820.11024973016957931No Hit
GGAAACCTTGTTACGACTTTTACTTCCTCTAGATGACTTAGTTCGTTTCA178320.10933006275074733No Hit
GGTTACCTTGTTACGACTTCACCCCAGTTACCAGTCACTCTTTAGGCGGC177390.1087598689510715No Hit
TGATACCTTGTTACGACTTCTCCTTCCTCTAGAAATTGAGGTTTGAACAA174460.10696345192628633No Hit
GGAAACCTTGTTACGACTTTTACTTCCTCTAAATGAGCAAGTTTGATCTA171920.10540614843039747No Hit
GGAAACCTTGTTACGACTTCTTCTTCCTCTAGACGTCCAAATTTACAAAG171720.10528352610788656No Hit
GGCTACCTTGTTACGACTTCACCCCAATCACCGACCATACCTTCGGCGCC169690.10403890953440059No Hit
GGCTACCTTGTTACGACTTCACCCCAGTCGCTGATCCCACCGTGGTCAGC168370.10322960220582843No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTTACG14469600.094.9596259
CTTGTTA13247900.094.954477
CCTTGTT13274600.094.915916
TTGTTAC13244500.094.878788
ATGTTAC1110350.094.8074958
CATGTTA1111550.094.76077
AAACCTT10188150.094.664323
AGATACC1266850.094.576311
ACCATGT1113950.094.569335
AACCTTG10324050.094.545884
CCATGTT1115050.094.535676
GAAACCT10168650.094.388082
GGAAACC10159600.093.820861
ACCTTGT13469800.093.7689365
GATACCT654600.093.744652
CGTGTTA130400.093.615797
GACCTTG921500.092.963914
TTGTGAC91050.092.80898
GTTACCT477000.092.351162
GATACCA1126200.092.3344042
Produced by FastQC (version 0.11.2)