##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename 000000000-AH092 l01n02.3320000000e718.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 16310244 Sequences flagged as poor quality 0 Sequence length 12 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.743459631873073 33.0 32.0 34.0 16.0 35.0 2 31.09502144787043 33.0 32.0 34.0 16.0 35.0 3 31.234937012591597 33.0 32.0 34.0 16.0 35.0 4 31.27677317396355 33.0 32.0 34.0 16.0 35.0 5 31.244596524736234 33.0 32.0 34.0 16.0 35.0 6 33.20213468296367 37.0 33.0 37.0 18.0 37.0 7 32.82184061746716 35.0 33.0 37.0 18.0 37.0 8 32.913661561408894 35.0 33.0 37.0 18.0 37.0 9 33.2702247740745 37.0 33.0 37.0 18.0 37.0 10 33.04394698203166 35.0 33.0 37.0 18.0 37.0 11 33.21200884548386 37.0 33.0 37.0 18.0 37.0 12 33.06685068598606 37.0 33.0 37.0 18.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.2557772942129297 1101 2 -0.08318678385897371 1101 3 0.0767612615536919 1101 4 0.10574867857268089 1101 5 0.20640997228039026 1101 6 0.15779207907528559 1101 7 0.2732970820644596 1101 8 0.2810065469343783 1101 9 0.209355550289537 1101 10 0.22448339160176545 1101 11 0.19290737793969726 1101 12 0.25561336627431785 1102 1 0.2984951867927066 1102 2 0.3086394710865683 1102 3 0.3164275185613441 1102 4 0.290057658045928 1102 5 -0.0101019461299785 1102 6 -0.4253035967967733 1102 7 0.17990520703392576 1102 8 -0.22750834329436742 1102 9 -0.02195810003244958 1102 10 -0.3198805137037226 1102 11 0.14077603449135268 1102 12 -0.10896104938159112 1103 1 0.3810233038444366 1103 2 0.1998101717827261 1103 3 0.29957883059055135 1103 4 0.222242224824857 1103 5 0.19170607218046953 1103 6 0.2514025941856133 1103 7 0.38951585930520594 1103 8 0.09919326775469983 1103 9 0.1570476680106978 1103 10 0.2963799907757121 1103 11 0.3019282104035099 1103 12 0.21271237608547722 2112 1 0.38543480908001015 2112 2 0.3465000065729491 2112 3 0.19037869443936373 2112 4 0.23865701339911993 2112 5 0.18322199177107024 2112 6 0.279407956369063 2112 7 0.4224069555404455 2112 8 0.3990949523828533 2112 9 0.21208770882529393 2112 10 0.3164975911591128 2112 11 0.3723633552932313 2112 12 0.30689897455799553 1113 1 0.11177075576491546 1113 2 0.16088156318232905 1113 3 0.23881116679023506 1113 4 0.23869653245504097 1113 5 0.25884203476743295 1113 6 0.31763694272180487 1113 7 0.3970955431611287 1113 8 0.3197461887253965 1113 9 0.2254364344542168 1113 10 0.35606000698916773 1113 11 0.3360403512359298 1113 12 0.2483195611615301 2113 1 -0.15630470239968375 2113 2 0.14231787323686618 2113 3 0.13006750990432892 2113 4 0.15256742664759315 2113 5 0.1395797017932452 2113 6 0.31917442609726265 2113 7 0.2517018956515571 2113 8 0.08746202370227962 2113 9 0.03557097502332596 2113 10 0.264098219568055 2113 11 0.13770292176418053 2113 12 0.0753501912618546 1112 1 0.23338542397148743 1112 2 0.18753129557221015 1112 3 0.2040625688736455 1112 4 0.19615245998311082 1112 5 0.30641317219591 1112 6 0.14131887985209346 1112 7 0.22477611223337846 1112 8 0.07685743632008979 1112 9 0.08112108099315662 1112 10 0.1703948565121749 1112 11 0.239561381834136 1112 12 0.12155099552522586 2114 1 0.23827810119748705 2114 2 0.30023904812748015 2114 3 0.2583416522871076 2114 4 0.23965447452037125 2114 5 0.1897415181943245 2114 6 0.22912380308893177 2114 7 0.18912670408816723 2114 8 0.02299888548621709 2114 9 0.2186052171595776 2114 10 0.24408092059390896 2114 11 0.2566054669137898 2114 12 0.026752037266994932 1114 1 0.20767705638536427 1114 2 0.1616745236277275 1114 3 -0.01481489881891207 1114 4 0.12365137303951812 1114 5 -0.0699439645911859 1114 6 -0.08682964193047837 1114 7 -0.10496301034393696 1114 8 0.07255153983699358 1114 9 0.12081802767445993 1114 10 0.1464632323835815 1114 11 0.12993317543992333 1114 12 0.19480994843354438 1109 1 0.06861602428705282 1109 2 0.16472631813832805 1109 3 0.26828556275147264 1109 4 0.20687890168780854 1109 5 0.26074210959716027 1109 6 0.20755393890866713 1109 7 0.18963701082034135 1109 8 0.3266174592344129 1109 9 0.2794764579827742 1109 10 0.3013108056510134 1109 11 0.14165413502662716 1109 12 0.34177795121856036 1108 1 0.34749227855529696 1108 2 0.26798302943890917 1108 3 0.19762647537888256 1108 4 0.24342393456435119 1108 5 0.13235322308862862 1108 6 0.09583005183942817 1108 7 0.22626463179592093 1108 8 0.30591802841888693 1108 9 0.2619177695519923 1108 10 0.07102480238862796 1108 11 0.006280450438659102 1108 12 0.26807920907180716 1111 1 0.1748628484022774 1111 2 0.14892778414442986 1111 3 0.19837124672161366 1111 4 0.01817338702962701 1111 5 0.04691319470926558 1111 6 0.08539990110257634 1111 7 0.047253238757427596 1111 8 0.18697532273895945 1111 9 0.03635068036062705 1111 10 -0.06347811205803566 1111 11 -0.18439165902584875 1111 12 0.15851658253890122 1110 1 0.3170262529706811 1110 2 0.23481755642993463 1110 3 0.2698680054201503 1110 4 0.12658970215669285 1110 5 0.2024092083679676 1110 6 0.16427500242433268 1110 7 0.1559349819204101 1110 8 0.08846862369083652 1110 9 0.15919578939416112 1110 10 0.14632494673934815 1110 11 0.10228588171513309 1110 12 0.02297892951249736 1105 1 0.32878509872677597 1105 2 0.17908169864590562 1105 3 0.20834108175467847 1105 4 0.1669860393390188 1105 5 0.10156484848108249 1105 6 0.06290540341133521 1105 7 0.18628394136502635 1105 8 0.191691010192784 1105 9 0.2131491357440538 1105 10 0.09120702819517135 1105 11 0.056075111175616144 1105 12 0.13891523168349096 1104 1 -0.7531426224959503 1104 2 -0.49486636529260863 1104 3 -0.4409511178490071 1104 4 -0.21851195539541024 1104 5 -0.11709603029570914 1104 6 -0.04598712106695757 1104 7 0.0903252249358033 1104 8 0.04751661854614753 1104 9 -0.0025043350902222983 1104 10 0.0687943855819384 1104 11 -0.023164705793362828 1104 12 0.05054099148054547 1107 1 -0.20681420224815383 1107 2 -0.09378657859971185 1107 3 -0.14669860189684059 1107 4 -0.1519280606313096 1107 5 -0.09948159369755771 1107 6 -0.06238878016107918 1107 7 -0.22424757668005668 1107 8 -0.029932124247139313 1107 9 -0.0799096733324447 1107 10 -0.037823464178529775 1107 11 -0.07070838349130071 1107 12 -0.030029739729641847 1106 1 -0.2631615109851779 1106 2 -0.06544703285726428 1106 3 -0.10154241889841131 1106 4 -0.020994550007362278 1106 5 -0.03467703645005216 1106 6 0.026448881778776467 1106 7 -0.27808490500181193 1106 8 0.019544635292731982 1106 9 -0.028825325575738248 1106 10 -0.10725166846523138 1106 11 0.04080764116461211 1106 12 0.10443866974198102 2103 1 -0.41562170997965 2103 2 -0.13225619718011217 2103 3 -0.13341387068525634 2103 4 -0.10233596088172803 2103 5 -0.0593396129435213 2103 6 -0.00156116392020067 2103 7 -0.31241915312031665 2103 8 -0.005143071500263829 2103 9 -0.16140594686122256 2103 10 -0.1391079044829624 2103 11 0.034714588939735336 2103 12 0.06622777278817438 2102 1 0.07129802026937782 2102 2 0.07133482413149395 2102 3 -0.0230064903451499 2102 4 -0.18844498972013213 2102 5 -0.07405021642354015 2102 6 -0.004814756248649132 2102 7 -0.2815686856883062 2102 8 0.01867200928609236 2102 9 -0.1417111150063306 2102 10 -0.06632297819300703 2102 11 0.06531808032087127 2102 12 -0.060496484319337185 2101 1 -0.07617518654910427 2101 2 -0.25545929090583286 2101 3 -0.019428143749181004 2101 4 -0.02947904129883483 2101 5 0.01193473437792747 2101 6 0.01094436885652783 2101 7 -0.07075775974187337 2101 8 0.1158814412165654 2101 9 -0.034907715921690396 2101 10 0.00762316846641653 2101 11 -0.07864296207790744 2101 12 0.018342369736856767 2107 1 -0.07219549523272306 2107 2 -0.08240554622544138 2107 3 -0.2581247269227056 2107 4 -0.15228464040308864 2107 5 -0.12994903914520606 2107 6 -0.12420444126077967 2107 7 -0.06933204929265457 2107 8 0.0857695638632876 2107 9 -0.025103901367387493 2107 10 -0.09877754069952971 2107 11 -0.26741558921128217 2107 12 -0.1543074783258902 2106 1 -0.12884875612759572 2106 2 -0.1761160480533306 2106 3 -0.48405658520930217 2106 4 -0.21836505686991714 2106 5 -0.20974901257775613 2106 6 -0.12565340970134287 2106 7 -0.40168132080358276 2106 8 -0.10785539313494041 2106 9 -0.2597954175841224 2106 10 -0.18767615134687787 2106 11 -0.40177497340619794 2106 12 -0.3003991780364714 2105 1 -0.1832155677306737 2105 2 -0.24416988996797784 2105 3 -0.10831354802779103 2105 4 -0.20353469798634194 2105 5 8.253825383377489E-4 2105 6 0.023873470254216045 2105 7 -0.007400152385741876 2105 8 -0.1762458523486572 2105 9 -0.04951461365598675 2105 10 -0.027354239883429443 2105 11 -0.21287530339539273 2105 12 -0.28632433396499835 2104 1 -0.14226789819002406 2104 2 -0.30046072234712895 2104 3 -0.3742281231677076 2104 4 -0.34397589023769726 2104 5 -0.2798206998741364 2104 6 -0.2033705349366315 2104 7 -0.34090528905857553 2104 8 -0.6245319310869277 2104 9 -0.37414626762883785 2104 10 -0.39594692802424447 2104 11 -0.2923061376552738 2104 12 -0.4754497553985857 2111 1 -0.3512487014920396 2111 2 -0.1498413946827739 2111 3 -0.15060226397556065 2111 4 -0.24576507587750385 2111 5 -0.12391434522078626 2111 6 -0.24658614270101253 2111 7 -0.1763488635032715 2111 8 -0.45133351421932133 2111 9 -0.26089806952650463 2111 10 -0.28016534203236176 2111 11 -0.22448932765905738 2111 12 -0.3649956417553568 2110 1 -0.11760425769337246 2110 2 -0.2592984852579434 2110 3 -0.29266550818999804 2110 4 -0.20772107384647498 2110 5 -0.4657710910037487 2110 6 -0.3970459585470465 2110 7 -0.38023526706953703 2110 8 -0.6453626822743672 2110 9 -0.2826039723333764 2110 10 -0.4307947984155689 2110 11 -0.35173022920965735 2110 12 -0.36637762329555557 2109 1 0.018464334239371993 2109 2 -0.18165315269390803 2109 3 -0.05673530657657011 2109 4 -0.2873886022317471 2109 5 -0.2913945448646089 2109 6 -0.25534381741214673 2109 7 -0.3329454626540951 2109 8 -0.3285675038891185 2109 9 -0.20137486387044845 2109 10 -0.43065045782981315 2109 11 -0.19183535061485202 2109 12 -0.3246586470822166 2108 1 -0.06023158915042259 2108 2 -0.35551767619468677 2108 3 -0.25233997071466163 2108 4 -0.19875021087818467 2108 5 -0.26736803112567387 2108 6 -0.393998335282852 2108 7 -0.2426348933295941 2108 8 -0.14948513762868032 2108 9 -0.28547317767706915 2108 10 -0.11951324729237456 2108 11 -0.25561954255696406 2108 12 -0.1398252270501601 >>END_MODULE >>Per sequence quality scores pass #Quality Count 14 41.0 15 2860.0 16 87409.0 17 186076.0 18 251184.0 19 301363.0 20 271583.0 21 300083.0 22 292967.0 23 256654.0 24 239382.0 25 234699.0 26 222674.0 27 221309.0 28 236154.0 29 254301.0 30 306076.0 31 393472.0 32 613792.0 33 1582737.0 34 3892750.0 35 5077954.0 36 1084724.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 17.42067132778639 28.89700485167481 32.52729388965609 21.1550299308827 2 22.656877481415975 31.286613492722733 23.99231427806966 22.064194747791632 3 12.844081302523739 25.469140743694574 21.04084402416052 40.645933929621165 4 35.21492995445071 20.12923902303362 19.142325522536634 25.513505499979033 5 33.69991276647977 20.863452441300083 16.603859513076564 28.832775279143586 6 21.853719662317744 32.669118867872236 21.526250618936174 23.950910850873843 7 20.40703989468214 22.230985630871004 30.952571892854575 26.40940258159228 8 14.488507958556598 30.416767523526932 27.72403037011586 27.370694147800613 9 14.703188989692611 36.21349870670236 25.24982458876765 23.833487714837375 10 31.70676661857419 31.932403954226558 11.974756478198609 24.38607294900064 11 19.898518992113175 19.30420538159944 20.29964113351094 40.49763449277644 12 18.741019447655106 29.87546354303467 30.19675242136169 21.18676458794853 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1469.0 1 1469.0 2 1469.0 3 1469.0 4 4894.0 5 8319.0 6 8319.0 7 8319.0 8 8319.0 9 8319.0 10 8319.0 11 8319.0 12 8319.0 13 19215.5 14 30112.0 15 30112.0 16 30112.0 17 30112.0 18 30112.0 19 30112.0 20 30112.0 21 59284.5 22 88457.0 23 88457.0 24 88457.0 25 88457.0 26 88457.0 27 88457.0 28 88457.0 29 191631.5 30 294806.0 31 294806.0 32 294806.0 33 294806.0 34 294806.0 35 294806.0 36 294806.0 37 294806.0 38 3075661.5 39 5856517.0 40 5856517.0 41 5856517.0 42 5856517.0 43 5856517.0 44 5856517.0 45 5856517.0 46 5541521.0 47 5226525.0 48 5226525.0 49 5226525.0 50 5226525.0 51 5226525.0 52 5226525.0 53 5226525.0 54 4877604.5 55 4528684.0 56 4528684.0 57 4528684.0 58 4528684.0 59 4528684.0 60 4528684.0 61 4528684.0 62 4528684.0 63 2368231.0 64 207778.0 65 207778.0 66 207778.0 67 207778.0 68 207778.0 69 207778.0 70 207778.0 71 129927.5 72 52077.0 73 52077.0 74 52077.0 75 52077.0 76 52077.0 77 52077.0 78 52077.0 79 32136.0 80 12195.0 81 12195.0 82 12195.0 83 12195.0 84 12195.0 85 12195.0 86 12195.0 87 12195.0 88 7244.5 89 2294.0 90 2294.0 91 2294.0 92 2294.0 93 2294.0 94 2294.0 95 2294.0 96 1652.5 97 1011.0 98 1011.0 99 1011.0 100 1011.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 12 1.6310244E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 3.844226871685699 #Duplication Level Percentage of deduplicated Percentage of total 1 72.5428853770639 2.788713093161246 2 11.935269794286752 0.9176376972803155 3 4.294098440955764 0.49522465849157454 4 2.6159502764262927 0.40225225390526353 5 1.6068700394669226 0.3088586492512702 6 0.9679497156448807 0.22326109843935496 7 0.785619967697556 0.21140709735290572 8 0.7471176080468065 0.22976716681304618 9 0.5101546731001085 0.17650352727427238 >10 3.346026829239926 2.5225664975381945 >50 0.30886646361117287 0.82780354831059 >100 0.24991456548463512 2.1141739962762935 >500 0.0594323641985278 1.587271753800008 >1k 0.023826509957266054 1.5005570870480018 >5k 3.1670393787819166E-4 0.07742575433069607 >10k+ 0.0057006708817719165 85.61657612072699 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source TTCAGACTACCA 1286594 7.888257220431527 No Hit CACGCCATAATG 813176 4.985676486507498 No Hit TCCGACACAATT 611477 3.74903649510087 No Hit AGCAGTCGCGAT 607933 3.727307819551933 No Hit AACGGATAAACG 554947 3.40244450052372 No Hit AGTTCAGACGCT 515011 3.15759224693389 No Hit GCGGATGTGACT 497315 3.0490960159762173 No Hit GTATGCGCTGTA 443819 2.7211058277239752 No Hit AATCCGTACAGC 435565 2.6704995952237134 No Hit CAGGGTGATACC 405797 2.4879885303984417 No Hit CTAACCTCCGCT 405208 2.4843773030004948 No Hit CCTCGTTCGACT 402360 2.466915884274938 No Hit CCAGTGTATGCA 401212 2.4598773629628106 No Hit TATCGTTGACCA 392299 2.405230724935813 No Hit GAGTGAGTACAA 388249 2.3803997046273495 No Hit TGCCCATTTAAG 386936 2.372349549154507 No Hit ATTTCGACATGC 378212 2.318861692075238 No Hit TGAGAACCTCCA 369667 2.266471304782442 No Hit AATCAGTCTCGT 361604 2.21703611546216 No Hit GGAGACAAGGGA 357869 2.194136396733243 No Hit TAATGACCACGC 347413 2.1300294465245275 No Hit TACACGATCTAC 345871 2.120575265458935 No Hit TTACTGTGCGAT 342846 2.1020286391791565 No Hit ACACCTGGTGAT 326828 2.0038204210801505 No Hit TGCCTACAAGCA 304936 1.8695980268596841 No Hit ACCGTGCATAGG 288439 1.7684530041365414 No Hit ATCGAATGCGGC 284271 1.7428985121252631 No Hit AGGCTACACGAC 269086 1.6497975137588377 No Hit GCACACACGTTA 262493 1.609375065143109 No Hit CAGGCGTATTGG 239208 1.4666120261597557 No Hit CTTATCAAGGCG 210232 1.2889568053059168 No Hit GGATCGCAGATC 195885 1.2009936822526996 No Hit GACTTGGTATTC 189052 1.1590997657668396 No Hit TGAGTCACTGGT 173306 1.0625592112539823 No Hit GCTGATGAGCTG 162827 0.9983112453743794 No Hit AGCTGTTGTTTG 106592 0.6535279300542653 No Hit >>END_MODULE >>Adapter Content warn >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTGGCGA 55 0.009160763 6.0000005 5 GCCCACG 90 9.15256E-6 6.0000005 5 GGTGTAT 90 9.15256E-6 6.0000005 3 GACACAG 90 9.15256E-6 6.0000005 4 CCAGGGA 95 3.4298992E-6 6.0000005 6 CATGACC 95 3.4298992E-6 6.0000005 2 CTCCAAG 90 9.15256E-6 6.0000005 4 TAGTTCG 95 3.4298992E-6 6.0000005 3 AATGCCG 90 9.15256E-6 6.0000005 5 GAGCGTG 55 0.009160763 6.0000005 1 TACTGTA 90 9.15256E-6 6.0000005 2 TGCTGCA 55 0.009160763 6.0000005 6 CAGGCCA 55 0.009160763 6.0000005 1 TCCAATG 55 0.009160763 6.0000005 2 AGTTCAT 180 0.0 6.0000005 1 TCTCAAG 110 1.8153878E-7 6.0000005 3 TGGGCGA 55 0.009160763 6.0000005 5 GCTACAT 55 0.009160763 6.0000005 3 GGCGACC 55 0.009160763 6.0000005 1 CAGAGCT 55 0.009160763 6.0000005 5 >>END_MODULE