FastQCFastQC Report
Mon 20 Jul 2015
000000000-AH092 l01n02.3320000000e718.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AH092 l01n02.3320000000e718.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16310244
Sequences flagged as poor quality0
Sequence length12
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCAGACTACCA12865947.888257220431527No Hit
CACGCCATAATG8131764.985676486507498No Hit
TCCGACACAATT6114773.74903649510087No Hit
AGCAGTCGCGAT6079333.727307819551933No Hit
AACGGATAAACG5549473.40244450052372No Hit
AGTTCAGACGCT5150113.15759224693389No Hit
GCGGATGTGACT4973153.0490960159762173No Hit
GTATGCGCTGTA4438192.7211058277239752No Hit
AATCCGTACAGC4355652.6704995952237134No Hit
CAGGGTGATACC4057972.4879885303984417No Hit
CTAACCTCCGCT4052082.4843773030004948No Hit
CCTCGTTCGACT4023602.466915884274938No Hit
CCAGTGTATGCA4012122.4598773629628106No Hit
TATCGTTGACCA3922992.405230724935813No Hit
GAGTGAGTACAA3882492.3803997046273495No Hit
TGCCCATTTAAG3869362.372349549154507No Hit
ATTTCGACATGC3782122.318861692075238No Hit
TGAGAACCTCCA3696672.266471304782442No Hit
AATCAGTCTCGT3616042.21703611546216No Hit
GGAGACAAGGGA3578692.194136396733243No Hit
TAATGACCACGC3474132.1300294465245275No Hit
TACACGATCTAC3458712.120575265458935No Hit
TTACTGTGCGAT3428462.1020286391791565No Hit
ACACCTGGTGAT3268282.0038204210801505No Hit
TGCCTACAAGCA3049361.8695980268596841No Hit
ACCGTGCATAGG2884391.7684530041365414No Hit
ATCGAATGCGGC2842711.7428985121252631No Hit
AGGCTACACGAC2690861.6497975137588377No Hit
GCACACACGTTA2624931.609375065143109No Hit
CAGGCGTATTGG2392081.4666120261597557No Hit
CTTATCAAGGCG2102321.2889568053059168No Hit
GGATCGCAGATC1958851.2009936822526996No Hit
GACTTGGTATTC1890521.1590997657668396No Hit
TGAGTCACTGGT1733061.0625592112539823No Hit
GCTGATGAGCTG1628270.9983112453743794No Hit
AGCTGTTGTTTG1065920.6535279300542653No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGGCGA550.0091607636.00000055
GCCCACG909.15256E-66.00000055
GGTGTAT909.15256E-66.00000053
GACACAG909.15256E-66.00000054
CCAGGGA953.4298992E-66.00000056
CATGACC953.4298992E-66.00000052
CTCCAAG909.15256E-66.00000054
TAGTTCG953.4298992E-66.00000053
AATGCCG909.15256E-66.00000055
GAGCGTG550.0091607636.00000051
TACTGTA909.15256E-66.00000052
TGCTGCA550.0091607636.00000056
CAGGCCA550.0091607636.00000051
TCCAATG550.0091607636.00000052
AGTTCAT1800.06.00000051
TCTCAAG1101.8153878E-76.00000053
TGGGCGA550.0091607636.00000055
GCTACAT550.0091607636.00000053
GGCGACC550.0091607636.00000051
CAGAGCT550.0091607636.00000055
Produced by FastQC (version 0.11.2)